Daam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Daam1
Entrez Gene ID	314212
Full Name	dishevelled associated activator of morphogenesis 1
General protein information	Preferred Names dishevelled associated activator of morphogenesis 1 Names disheveled-associated activator of morphogenesis 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108030.1	NP_001101500.1	disheveled-associated activator of morphogenesis 1
XM_006240212.3	XP_006240274.1	disheveled-associated activator of morphogenesis 1 isoform X2
XM_006240211.3	XP_006240273.1	disheveled-associated activator of morphogenesis 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DAAM1	NP_001075057.1
Xenopus tropicalis (tropical clawed frog)	daam1	XP_004917272.1
	DAAM1	XP_001093845.1
Drosophila melanogaster (fruit fly)	DAAM	NP_726724.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003292	XP_307797.5
Homo sapiens (human)	DAAM1	NP_055807.1
Mus musculus (house mouse)	Daam1	NP_001273381.1
Gallus gallus (chicken)	DAAM1	NP_001180553.1
Oryza sativa (rice)	Os10g0119300	NP_001064063.1
Pan troglodytes (chimpanzee)	DAAM1	XP_003314344.1
Canis lupus familiaris (dog)	DAAM1	XP_852088.1
Rattus norvegicus (Norway rat)	Daam1	NP_001101500.1
Danio rerio (zebrafish)	daam1b	NP_001025307.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of testicular DAAM1 expression in zinc protection against cadmium-induced male rat reproductive toxicity.
Chemek M, Venditti M, Boughamoura S, Mimouna SB, Messaoudi I, Minucci S
Journal of cellular physiology233(1)630-640(2018 Jan)

First Evidence of DAAM1 Localization During the Post-Natal Development of Rat Testis and in Mammalian Sperm.
Pariante P, Dotolo R, Venditti M, Ferrara D, Donizetti A, Aniello F, Minucci S
Journal of cellular physiology231(10)2172-84(2016 Oct)

The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells.
Yasunaga T, Hoff S, Schell C, Helmst?dter M, Kretz O, Kuechlin S, Yakulov TA, Engel C, M?ller B, Bensch R, Ronneberger O, Huber TB, Lienkamp SS, Walz G
The Journal of cell biology211(5)963-73(2015 Dec)

Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1.
Wagh D, Terry-Lorenzo R, Waites CL, Leal-Ortiz SA, Maas C, Reimer RJ, Garner CC
PloS one10(4)e0120093(2015)

Planar cell polarity pathway regulates actin rearrangement, cell shape, motility, and nephrin distribution in podocytes.
Babayeva S, Zilber Y, Torban E
American journal of physiology. Renal physiology300(2)F549-60(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zinc chloride could prevent cadmium chloride-induced testicular toxicity and sperm quality alteration in adult male rat after gestational and lactational exposure, probably via the restoration of the testicular DAAM1 expression inhibited by Cd.
Title: Involvement of testicular DAAM1 expression in zinc protection against cadmium-induced male rat reproductive toxicity.

a role for DAAM1 in cytoskeletal organization during Mammalian testis morphogenesis and gamete progression
Title: First Evidence of DAAM1 Localization During the Post-Natal Development of Rat Testis and in Mammalian Sperm.

Similar to Piccolo and Profilin, Daam1 loss of function impairs presynaptic-F-actin assembly in neurons.
Title: Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1.

The following Daam1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Daam1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06541	NM_001108030.1 Latest version!	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa29404	XM_006240211.3 Latest version!	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa29405	XM_006240212.3 Latest version!	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Daam1 ( NM_001108030.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101500.1 Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), mRNA.

Price & Availability↑

CloneID	ORa06541
Clone ID Related Accession (Same CDS sequence)	NM_001108030.1
Accession Version	NM_001108030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Rattus norvegicus(Norway rat)
Product	disheveled-associated activator of morphogenesis 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473947.1. On or before Oct 4, 2007 this sequence version replaced XM_234275.4, XM_001080564.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02642799, SAMN02642861 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGAA AGAAGCAGAT 
GCCATTGATG ACGCACTGAG TTCCAAACTT AAAGTCAAAG AGCTGTCAGT GATTGATGGT 
CGGAGAGCTC AGAATTGCAA CATCCTTCTG TCGAGGTTGA AGTTATCCAA TGACGAAATC 
AAGCGAGCAA TTCTAACAAT GGATGAACAG GAGGACCTGC CCAAGGACAT GTTAGAACAG 
CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC ATTGACCTGC TAGAAGAACA TAAACATGAG 
TTGGACCGGA TGGCCAAGGC TGATCGCTTC CTGTTTGAGA TGAGCAGAAT TAATCATTAC 
CAGCAACGAC TGCAATCACT GTACTTCAAA AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG 
AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC TCAGAGGAGG TACTCAGGAG CAGTGCCCTC 
AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT GGAAATTATA TGAATAAAGG ACAAAGAGGC 
AATGCATATG GATTCAAGAT CTCCAGCCTC AACAAGATTG CAGATACAAA ATCCAGTATT 
GACAAGAACA TTACCCTCTT GCACTATCTA ATAACCATTG TGGAGAATAA GTACCCCAAG 
GTCCTGAATC TCAGCGAAGA GTTGCGGGAT ATTCCTCAAG CAGCGAAAGT GAACATGACT 
GAGCTGGACA AAGAGATCAG CACCTTGAGG AGTGGCCTGA AAGCTGTGGA GACGGAACTT 
GAGTATCAAA AGTCTCAGCC CCCGCAGCCT GGAGACAAGT TTGTGTCTGT TGTCAGCCAG 
TTTATCACCC TGGCGAGCTT CAGCTTCTCA GACGTAGAAG ATCTTCTAGC AGAAGCTAAA 
GAGCTGTTCA CTAAAGCAGT GAAGCACTTT GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT 
GAGTTCTTTG GGATCTTTGA TCAGTTTCTT CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT 
GAGAACATGA GGAAAAGGAA GGAGGAGGAG GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC 
AAAGAGCAGC GGGAGAGGGA GCGGAAGATG AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT 
GGCGAGTTTG ACGACCTGGT CTCGGCTCTG CGCTCAGGAG AGGTGTTCGA CAAAGACCTT 
TCAAAACTCA AACGGAACCG GAAACGCATT TCCAACCAGG TGACGGACAG CAGCCGCGAG 
CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101500.1
CDS	332..3238
Translation	MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELNRNKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQKEADAIDDALSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSSSEEVLRSSALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPKVLNLSEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITLASFSFSDVEDLLAEAKELFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVTEAKQENENMRKRKEEEERRARLEAQLKEQRERERKMRKAKESSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRISNQVTDSSRERPITKLNF

Target ORF information:

RefSeq Version	NM_001108030.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108030.1

ORF Insert Sequence:

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGAA AGAAGCAGAT 
GCCATTGATG ACGCACTGAG TTCCAAACTT AAAGTCAAAG AGCTGTCAGT GATTGATGGT 
CGGAGAGCTC AGAATTGCAA CATCCTTCTG TCGAGGTTGA AGTTATCCAA TGACGAAATC 
AAGCGAGCAA TTCTAACAAT GGATGAACAG GAGGACCTGC CCAAGGACAT GTTAGAACAG 
CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC ATTGACCTGC TAGAAGAACA TAAACATGAG 
TTGGACCGGA TGGCCAAGGC TGATCGCTTC CTGTTTGAGA TGAGCAGAAT TAATCATTAC 
CAGCAACGAC TGCAATCACT GTACTTCAAA AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG 
AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC TCAGAGGAGG TACTCAGGAG CAGTGCCCTC 
AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT GGAAATTATA TGAATAAAGG ACAAAGAGGC 
AATGCATATG GATTCAAGAT CTCCAGCCTC AACAAGATTG CAGATACAAA ATCCAGTATT 
GACAAGAACA TTACCCTCTT GCACTATCTA ATAACCATTG TGGAGAATAA GTACCCCAAG 
GTCCTGAATC TCAGCGAAGA GTTGCGGGAT ATTCCTCAAG CAGCGAAAGT GAACATGACT 
GAGCTGGACA AAGAGATCAG CACCTTGAGG AGTGGCCTGA AAGCTGTGGA GACGGAACTT 
GAGTATCAAA AGTCTCAGCC CCCGCAGCCT GGAGACAAGT TTGTGTCTGT TGTCAGCCAG 
TTTATCACCC TGGCGAGCTT CAGCTTCTCA GACGTAGAAG ATCTTCTAGC AGAAGCTAAA 
GAGCTGTTCA CTAAAGCAGT GAAGCACTTT GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT 
GAGTTCTTTG GGATCTTTGA TCAGTTTCTT CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT 
GAGAACATGA GGAAAAGGAA GGAGGAGGAG GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC 
AAAGAGCAGC GGGAGAGGGA GCGGAAGATG AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT 
GGCGAGTTTG ACGACCTGGT CTCGGCTCTG CGCTCAGGAG AGGTGTTCGA CAAAGACCTT 
TCAAAACTCA AACGGAACCG GAAACGCATT TCCAACCAGG TGACGGACAG CAGCCGCGAG 
CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Daam1 ( XM_006240211.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240273.1 Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29404
Clone ID Related Accession (Same CDS sequence)	XM_006240211.3
Accession Version	XM_006240211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	disheveled-associated activator of morphogenesis 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AGGGCCGTCT GTGCCTCAGT CCCAGGAGGA CCCTCACCAG GAGCCCCAGG AGGACCCTTT 
CCTCCCTCTG GGTTGGGCTC TCTTCTCCCT CCCCCACCAC CCCCTCTCCT TTCAGGTGGA 
GCACTTCCTC CTCCACCACC TCCCCTCCCT CCTGGGGGTC CTCCTCCTCC CCCAGGGCCT 
CCTCCCTTAG GAGGAATCCT GCCACCTCCT GGTGCTCCAG TGAGTCTGAC ACTCAAGAAG 
AAAAATATTC CCCAGCCCAC CAATGCCCTG AAATCCTTCA ACTGGTCCAA GTTGCCTGAG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGGA GTTCTTTGTG 
AACAATAACT CCAAGCAGAA AGAAGCAGAT GCCATTGATG ACGCACTGAG TTCCAAACTT 
AAAGTCAAAG AGCTGTCAGT GATTGATGGT CGGAGAGCTC AGAATTGCAA CATCCTTCTG 
TCGAGGTTGA AGTTATCCAA TGACGAAATC AAGCGAGCAA TTCTAACAAT GGATGAACAG 
GAGGACCTGC CCAAGGACAT GTTAGAACAG CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC 
ATTGACCTGC TAGAAGAACA TAAACATGAG TTGGACCGGA TGGCCAAGGC TGATCGCTTC 
CTGTTTGAGA TGAGCAGAAT TAATCATTAC CAGCAACGAC TGCAATCACT GTACTTCAAA 
AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC 
TCAGAGGAGG TACTCAGGAG CAGTGCCCTC AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT 
GGAAATTATA TGAATAAAGG ACAAAGAGGC AATGCATATG GATTCAAGAT CTCCAGCCTC 
AACAAGATTG CAGATACAAA ATCCAGTATT GACAAGAACA TTACCCTCTT GCACTATCTA 
ATAACCATTG TGGAGAATAA GTACCCCAAG GTCCTGAATC TCAGCGAAGA GTTGCGGGAT 
ATTCCTCAAG CAGCGAAAGT GAACATGACT GAGCTGGACA AAGAGATCAG CACCTTGAGG 
AGTGGCCTGA AAGCTGTGGA GACGGAACTT GAGTATCAAA AGTCTCAGCC CCCGCAGCCT 
GGAGACAAGT TTGTGTCTGT TGTCAGCCAG TTTATCACCC TGGCGAGCTT CAGCTTCTCA 
GACGTAGAAG ATCTTCTAGC AGAAGCTAAA GAGCTGTTCA CTAAAGCAGT GAAGCACTTT 
GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT GAGTTCTTTG GGATCTTTGA TCAGTTTCTT 
CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT GAGAACATGA GGAAAAGGAA GGAGGAGGAG 
GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC AAAGAGCAGC GGGAGAGGGA GCGGAAGATG 
AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT GGCGAGTTTG ACGACCTGGT CTCGGCTCTG 
CGCTCAGGAG AGGTGTTCGA CAAAGACCTT TCAAAACTCA AACGGAACCG GAAACGCATT 
TCCAACCAGG TGACGGACAG CAGCCGCGAG CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240273.1
CDS	4586..7822
Translation	MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELNRRAVCASVPGGPSPGAPGGPFPPSGLGSLLPPPPPPLLSGGALPPPPPPLPPGGPPPPPGPPPLGGILPPPGAPVSLTLKKKNIPQPTNALKSFNWSKLPENKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQEFFVNNNSKQKEADAIDDALSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSSSEEVLRSSALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPKVLNLSEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITLASFSFSDVEDLLAEAKELFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVTEAKQENENMRKRKEEEERRARLEAQLKEQRERERKMRKAKESSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRISNQVTDSSRERPITKLNF

Target ORF information:

RefSeq Version	XM_006240211.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240211.3

ORF Insert Sequence:

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AGGGCCGTCT GTGCCTCAGT CCCAGGAGGA CCCTCACCAG GAGCCCCAGG AGGACCCTTT 
CCTCCCTCTG GGTTGGGCTC TCTTCTCCCT CCCCCACCAC CCCCTCTCCT TTCAGGTGGA 
GCACTTCCTC CTCCACCACC TCCCCTCCCT CCTGGGGGTC CTCCTCCTCC CCCAGGGCCT 
CCTCCCTTAG GAGGAATCCT GCCACCTCCT GGTGCTCCAG TGAGTCTGAC ACTCAAGAAG 
AAAAATATTC CCCAGCCCAC CAATGCCCTG AAATCCTTCA ACTGGTCCAA GTTGCCTGAG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGGA GTTCTTTGTG 
AACAATAACT CCAAGCAGAA AGAAGCAGAT GCCATTGATG ACGCACTGAG TTCCAAACTT 
AAAGTCAAAG AGCTGTCAGT GATTGATGGT CGGAGAGCTC AGAATTGCAA CATCCTTCTG 
TCGAGGTTGA AGTTATCCAA TGACGAAATC AAGCGAGCAA TTCTAACAAT GGATGAACAG 
GAGGACCTGC CCAAGGACAT GTTAGAACAG CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC 
ATTGACCTGC TAGAAGAACA TAAACATGAG TTGGACCGGA TGGCCAAGGC TGATCGCTTC 
CTGTTTGAGA TGAGCAGAAT TAATCATTAC CAGCAACGAC TGCAATCACT GTACTTCAAA 
AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC 
TCAGAGGAGG TACTCAGGAG CAGTGCCCTC AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT 
GGAAATTATA TGAATAAAGG ACAAAGAGGC AATGCATATG GATTCAAGAT CTCCAGCCTC 
AACAAGATTG CAGATACAAA ATCCAGTATT GACAAGAACA TTACCCTCTT GCACTATCTA 
ATAACCATTG TGGAGAATAA GTACCCCAAG GTCCTGAATC TCAGCGAAGA GTTGCGGGAT 
ATTCCTCAAG CAGCGAAAGT GAACATGACT GAGCTGGACA AAGAGATCAG CACCTTGAGG 
AGTGGCCTGA AAGCTGTGGA GACGGAACTT GAGTATCAAA AGTCTCAGCC CCCGCAGCCT 
GGAGACAAGT TTGTGTCTGT TGTCAGCCAG TTTATCACCC TGGCGAGCTT CAGCTTCTCA 
GACGTAGAAG ATCTTCTAGC AGAAGCTAAA GAGCTGTTCA CTAAAGCAGT GAAGCACTTT 
GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT GAGTTCTTTG GGATCTTTGA TCAGTTTCTT 
CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT GAGAACATGA GGAAAAGGAA GGAGGAGGAG 
GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC AAAGAGCAGC GGGAGAGGGA GCGGAAGATG 
AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT GGCGAGTTTG ACGACCTGGT CTCGGCTCTG 
CGCTCAGGAG AGGTGTTCGA CAAAGACCTT TCAAAACTCA AACGGAACCG GAAACGCATT 
TCCAACCAGG TGACGGACAG CAGCCGCGAG CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Daam1 ( XM_006240212.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240274.1 Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X2, mRNA.

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CloneID	ORa29405
Clone ID Related Accession (Same CDS sequence)	XM_006240212.3
Accession Version	XM_006240212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	disheveled-associated activator of morphogenesis 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AGGGCCGTCT GTGCCTCAGT CCCAGGAGGA CCCTCACCAG GAGCCCCAGG AGGACCCTTT 
CCTCCCTCTG GGTTGGGCTC TCTTCTCCCT CCCCCACCAC CCCCTCTCCT TTCAGGTGGA 
GCACTTCCTC CTCCACCACC TCCCCTCCCT CCTGGGGGTC CTCCTCCTCC CCCAGGGCCT 
CCTCCCTTAG GAGGAATCCT GCCACCTCCT GGTGCTCCAG TGAGTCTGAC ACTCAAGAAG 
AAAAATATTC CCCAGCCCAC CAATGCCCTG AAATCCTTCA ACTGGTCCAA GTTGCCTGAG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGAA AGAAGCAGAT 
GCCATTGATG ACGCACTGAG TTCCAAACTT AAAGTCAAAG AGCTGTCAGT GATTGATGGT 
CGGAGAGCTC AGAATTGCAA CATCCTTCTG TCGAGGTTGA AGTTATCCAA TGACGAAATC 
AAGCGAGCAA TTCTAACAAT GGATGAACAG GAGGACCTGC CCAAGGACAT GTTAGAACAG 
CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC ATTGACCTGC TAGAAGAACA TAAACATGAG 
TTGGACCGGA TGGCCAAGGC TGATCGCTTC CTGTTTGAGA TGAGCAGAAT TAATCATTAC 
CAGCAACGAC TGCAATCACT GTACTTCAAA AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG 
AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC TCAGAGGAGG TACTCAGGAG CAGTGCCCTC 
AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT GGAAATTATA TGAATAAAGG ACAAAGAGGC 
AATGCATATG GATTCAAGAT CTCCAGCCTC AACAAGATTG CAGATACAAA ATCCAGTATT 
GACAAGAACA TTACCCTCTT GCACTATCTA ATAACCATTG TGGAGAATAA GTACCCCAAG 
GTCCTGAATC TCAGCGAAGA GTTGCGGGAT ATTCCTCAAG CAGCGAAAGT GAACATGACT 
GAGCTGGACA AAGAGATCAG CACCTTGAGG AGTGGCCTGA AAGCTGTGGA GACGGAACTT 
GAGTATCAAA AGTCTCAGCC CCCGCAGCCT GGAGACAAGT TTGTGTCTGT TGTCAGCCAG 
TTTATCACCC TGGCGAGCTT CAGCTTCTCA GACGTAGAAG ATCTTCTAGC AGAAGCTAAA 
GAGCTGTTCA CTAAAGCAGT GAAGCACTTT GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT 
GAGTTCTTTG GGATCTTTGA TCAGTTTCTT CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT 
GAGAACATGA GGAAAAGGAA GGAGGAGGAG GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC 
AAAGAGCAGC GGGAGAGGGA GCGGAAGATG AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT 
GGCGAGTTTG ACGACCTGGT CTCGGCTCTG CGCTCAGGAG AGGTGTTCGA CAAAGACCTT 
TCAAAACTCA AACGGAACCG GAAACGCATT TCCAACCAGG TGACGGACAG CAGCCGCGAG 
CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240274.1
CDS	4585..7791
Translation	MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELNRRAVCASVPGGPSPGAPGGPFPPSGLGSLLPPPPPPLLSGGALPPPPPPLPPGGPPPPPGPPPLGGILPPPGAPVSLTLKKKNIPQPTNALKSFNWSKLPENKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQKEADAIDDALSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSSSEEVLRSSALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPKVLNLSEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITLASFSFSDVEDLLAEAKELFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVTEAKQENENMRKRKEEEERRARLEAQLKEQRERERKMRKAKESSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRISNQVTDSSRERPITKLNF

Target ORF information:

RefSeq Version	XM_006240212.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240212.3

ORF Insert Sequence:

ATGGCCCCAA GGAAGAGAGG CGGAAGGGGG ATTTCATTTA TCTTTTGCTG TTTCCGAAAC 
AATGACCACC CAGAAATCAC ATATCGGCTT AGGAATGATA GCAACTTTGC ACTTCAGACA 
ATGGAGCCAG CACTGCCCAT GCCCCCTGTG GAGGAGCTGG ATGTCATGTT CAGTGAACTT 
GTGGATGAAC TCGACCTCAC AGACAAACAT AGGGAGGCCA TGTTTGCACT TCCAGCAGAG 
AAAAAGTGGC AGATATACTG TAGCAAGAAA AAGGACCAGG AAGAAAACAA GGGAGCAACA 
AGTTGGCCCG AGTTCTACAT TGATCAGCTC AATTCCATGG CTGCTAGAAA ATCTCTGCTG 
GCTTTAGAGA AGGAAGAAGA AGAAGAGAGG AGTAAAACCA TCGAGAGTCT AAAGACAGCA 
CTGAGGACAA AGCCCATGAG GTTTGTAACC AGATTCATCG ACTTGGATGG CCTGTCATGT 
ATTCTCAACT TTCTGAAGAC CATGGACTAT GAGACCTCCG AGTCTCGGAT ACATACCTCC 
CTCATTGGCT GTATAAAGGC ACTAATGAAC AACTCTCAAG GCCGGGCCCA CGTCCTGGCT 
CATTCTGAGA GTATTAATGT AATTGCTCAG AGTCTGAGCA CAGAGAACAT TAAAACAAAG 
GTGGCCGTGC TGGAAATCCT GGGCGCTGTG TGCCTGGTTC CTGGGGGCCA CAAGAAAGTT 
CTGCAGGCTA TGCTGCACTA TCAGAAGTAT GCCAGCGAGA GAACCCGCTT CCAGACGTTA 
ATCAACGACT TGGATAAAAG CACTGGGCGC TATCGGGATG AAGTGAGTCT CAAGACTGCC 
ATCATGTCGT TCATCAATGC AGTGCTGAGC CAGGGAGCCG GAGTGGAGAG TTTGGACTTC 
AGACTCCATC TTCGCTACGA ATTTCTGATG CTAGGAATTC AGCCTGTTAT AGATAAATTA 
AGGGAACATG AGAATTCAAC ACTAGACAGG CACTTAGACT TTTTTGAAAT GCTCCGAAAT 
GAAGATGAAC TAGAATTTGC CAAAAGATTT GAACTGGTTC ACATAGACAC AAAAAGCGCC 
ACTCAGATGT TTGAGCTGAC CAGGAGGAGG CTCACCCACA GCGAAGCCTA CCCCCACTTC 
ATGTCCATTC TGCACCATTG CCTGCAGATG CCTTACAAGA GGAGTGGTAA CACAGTCCAG 
TACTGGCTGC TGCTAGACAG AATCATACAG CAGATAGTTA TCCAGAATGA CAAAGGACAA 
GACCCCGACT CCACACCTCT GGAAAACTTT AATATTAAGA ACGTCGTAAG AATGCTGGTT 
AATGAAAACG AAGTTAAGCA ATGGAAAGAG CAAGCAGAGA AGATGAGGAA AGAGCACAAT 
GAGCTACAAC AGAAGCTGGA GAAGAAAGAG AGAGAATGTG ATGCCAAGAC CCAAGAGAAG 
GAAGAAATGA TGCAGACCTT AAACAAGATG AAGGAGAAGC TGGAGAAGGA GACCACTGAG 
CACAAGCAGG TTAAGCAGCA GGTGGCAGAC CTCACGGCGC AGCTCCACGA GCTCAACAGG 
AGGGCCGTCT GTGCCTCAGT CCCAGGAGGA CCCTCACCAG GAGCCCCAGG AGGACCCTTT 
CCTCCCTCTG GGTTGGGCTC TCTTCTCCCT CCCCCACCAC CCCCTCTCCT TTCAGGTGGA 
GCACTTCCTC CTCCACCACC TCCCCTCCCT CCTGGGGGTC CTCCTCCTCC CCCAGGGCCT 
CCTCCCTTAG GAGGAATCCT GCCACCTCCT GGTGCTCCAG TGAGTCTGAC ACTCAAGAAG 
AAAAATATTC CCCAGCCCAC CAATGCCCTG AAATCCTTCA ACTGGTCCAA GTTGCCTGAG 
AACAAATTGG AAGGAACAGT ATGGACTGAA ATCGATGATA CAAAAGTCTT CAAAATTCTA 
GACCTTGAAG ACCTAGAAAG AACATTCTCT GCCTACCAAA GACAGCAGAA AGAAGCAGAT 
GCCATTGATG ACGCACTGAG TTCCAAACTT AAAGTCAAAG AGCTGTCAGT GATTGATGGT 
CGGAGAGCTC AGAATTGCAA CATCCTTCTG TCGAGGTTGA AGTTATCCAA TGACGAAATC 
AAGCGAGCAA TTCTAACAAT GGATGAACAG GAGGACCTGC CCAAGGACAT GTTAGAACAG 
CTTTTGAAAT TCGTGCCTGA GAAAAGCGAC ATTGACCTGC TAGAAGAACA TAAACATGAG 
TTGGACCGGA TGGCCAAGGC TGATCGCTTC CTGTTTGAGA TGAGCAGAAT TAATCATTAC 
CAGCAACGAC TGCAATCACT GTACTTCAAA AAGAAGTTTG CAGAGCGTGT GGCCGAAGTG 
AAGCCCAAAG TAGAAGCGAT CCGCTCCAGC TCAGAGGAGG TACTCAGGAG CAGTGCCCTC 
AAGCAGCTCC TGGAGGTGGT TCTGGCTTTT GGAAATTATA TGAATAAAGG ACAAAGAGGC 
AATGCATATG GATTCAAGAT CTCCAGCCTC AACAAGATTG CAGATACAAA ATCCAGTATT 
GACAAGAACA TTACCCTCTT GCACTATCTA ATAACCATTG TGGAGAATAA GTACCCCAAG 
GTCCTGAATC TCAGCGAAGA GTTGCGGGAT ATTCCTCAAG CAGCGAAAGT GAACATGACT 
GAGCTGGACA AAGAGATCAG CACCTTGAGG AGTGGCCTGA AAGCTGTGGA GACGGAACTT 
GAGTATCAAA AGTCTCAGCC CCCGCAGCCT GGAGACAAGT TTGTGTCTGT TGTCAGCCAG 
TTTATCACCC TGGCGAGCTT CAGCTTCTCA GACGTAGAAG ATCTTCTAGC AGAAGCTAAA 
GAGCTGTTCA CTAAAGCAGT GAAGCACTTT GGGGAAGAGG CTGGCAAAAT CCAGCCAGAT 
GAGTTCTTTG GGATCTTTGA TCAGTTTCTT CAGGCTGTGA CAGAAGCGAA ACAGGAAAAT 
GAGAACATGA GGAAAAGGAA GGAGGAGGAG GAGCGGAGGG CTCGCCTGGA AGCTCAGCTC 
AAAGAGCAGC GGGAGAGGGA GCGGAAGATG AGGAAGGCCA AGGAGAGCAG TGAAGAGAGT 
GGCGAGTTTG ACGACCTGGT CTCGGCTCTG CGCTCAGGAG AGGTGTTCGA CAAAGACCTT 
TCAAAACTCA AACGGAACCG GAAACGCATT TCCAACCAGG TGACGGACAG CAGCCGCGAG 
CGACCAATCA CGAAACTTAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of testicular DAAM1 expression in zinc protection against cadmium-induced male rat reproductive toxicity.
Journal of cellular physiology233(1)630-640(2018 Jan)
Chemek M,Venditti M,Boughamoura S,Mimouna SB,Messaoudi I,Minucci S

First Evidence of DAAM1 Localization During the Post-Natal Development of Rat Testis and in Mammalian Sperm.
Journal of cellular physiology231(10)2172-84(2016 Oct)
Pariante P,Dotolo R,Venditti M,Ferrara D,Donizetti A,Aniello F,Minucci S

The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells.
The Journal of cell biology211(5)963-73(2015 Dec)
Yasunaga T,Hoff S,Schell C,Helmst?dter M,Kretz O,Kuechlin S,Yakulov TA,Engel C,M?ller B,Bensch R,Ronneberger O,Huber TB,Lienkamp SS,Walz G

Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1.
PloS one10(4)e0120093(2015)
Wagh D,Terry-Lorenzo R,Waites CL,Leal-Ortiz SA,Maas C,Reimer RJ,Garner CC

Planar cell polarity pathway regulates actin rearrangement, cell shape, motility, and nephrin distribution in podocytes.
American journal of physiology. Renal physiology300(2)F549-60(2011 Feb)
Babayeva S,Zilber Y,Torban E

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Daam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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