DAAM cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAAM
Entrez Gene ID	31075
Full Name	Dishevelled Associated Activator of Morphogenesis
Synonyms	CG14622,Daam,Dmel\CG14622,EG:114D9.2,anon-EST:Posey148,dDAAM,dDaam,daam
General protein information	Preferred Names Dishevelled Associated Activator of Morphogenesis Names CG14622 gene product from transcript CG14622-RF CG14622-PA CG14622-PB CG14622-PD CG14622-PE CG14622-PF CG14622-PG DAAM-PA DAAM-PB DAAM-PD DAAM-PE DAAM-PF DAAM-PG DIA-like dishevelled associated activator of morphogenesis formin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1F2-1F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_130544.4	NP_569900.3	dishevelled associated activator of morphogenesis, isoform A
NM_166875.3	NP_726723.2	dishevelled associated activator of morphogenesis, isoform F
NM_001297842.1	NP_001284771.1	dishevelled associated activator of morphogenesis, isoform G
NM_166876.3	NP_726724.1	dishevelled associated activator of morphogenesis, isoform B
NM_001272197.1	NP_001259126.1	dishevelled associated activator of morphogenesis, isoform E
NM_001201593.2	NP_001188522.1	dishevelled associated activator of morphogenesis, isoform D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DAAM1	NP_001075057.1
Xenopus tropicalis (tropical clawed frog)	daam1	XP_004917272.1
	DAAM1	XP_001093845.1
Drosophila melanogaster (fruit fly)	DAAM	NP_726724.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003292	XP_307797.5
Homo sapiens (human)	DAAM1	NP_055807.1
Mus musculus (house mouse)	Daam1	NP_001273381.1
Gallus gallus (chicken)	DAAM1	NP_001180553.1
Oryza sativa (rice)	Os10g0119300	NP_001064063.1
Pan troglodytes (chimpanzee)	DAAM1	XP_003314344.1
Canis lupus familiaris (dog)	DAAM1	XP_852088.1
Rattus norvegicus (Norway rat)	Daam1	NP_001101500.1
Danio rerio (zebrafish)	daam1b	NP_001025307.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that C-terminus, formin-homology domain contributes to DAAM-mediated filopodia formation and actin cytoskeletal dynamics in primary neurons.
Title: The activities of the C-terminal regions of the formin protein disheveled-associated activator of morphogenesis (DAAM) in actin dynamics.

Frl cooperates with DAAM during axon growth in the mushroom body
Title: Unique and Overlapping Functions of Formins Frl and DAAM During Ommatidial Rotation and Neuronal Development in Drosophila.

This study have shown that the formin dDAAM plays an important role in the regulation of axonal growth and guidance of the Drosophila MB neurons.
Title: The Formin DAAM Functions as Molecular Effector of the Planar Cell Polarity Pathway during Axonal Development in Drosophila.

Cdc42 and the formins diaphanous and DAAM play pivotal roles in heart lumen formation through the spatiotemporal regulation of the actomyosin network.
Title: Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis.

our data suggest that dDAAM is required for the initial assembly of thin filaments, and subsequently it promotes filament elongation by assembling short actin polymers
Title: DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.

The following DAAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00451	NM_166876.3 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant B (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00451	NM_166875.3 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant F (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00451	NM_001297842.1 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant G (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00451	NM_001272197.1 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant E (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00452	NM_130544.4 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant A (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa02960	NM_001201593.2 Latest version!	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant D (DAAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAAM ( NM_166875.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726723.2 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant F (DAAM), mRNA.

Price & Availability↑

CloneID	OFa00451
Clone ID Related Accession (Same CDS sequence)	NM_166875.3 , NM_001297842.1 , NM_166876.3 , NM_001272197.1
Accession Version	NM_166875.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_166875.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726723.2
CDS	963..4424
Translation	MPGFRGRRVWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_166875.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant F (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166875.3

ORF Insert Sequence:

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM ( NM_001297842.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284771.1 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant G (DAAM), mRNA.

Price & Availability↑

CloneID	OFa00451
Clone ID Related Accession (Same CDS sequence)	NM_166875.3 , NM_001297842.1 , NM_166876.3 , NM_001272197.1
Accession Version	NM_001297842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284771.1
CDS	1133..4594
Translation	MPGFRGRRVWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_001297842.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant G (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297842.1

ORF Insert Sequence:

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM ( NM_166876.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726724.1 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant B (DAAM), mRNA.

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CloneID	OFa00451
Clone ID Related Accession (Same CDS sequence)	NM_166875.3 , NM_001297842.1 , NM_166876.3 , NM_001272197.1
Accession Version	NM_166876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166876.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726724.1
CDS	1175..4636
Translation	MPGFRGRRVWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_166876.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant B (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166876.3

ORF Insert Sequence:

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM ( NM_001272197.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259126.1 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant E (DAAM), mRNA.

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CloneID	OFa00451
Clone ID Related Accession (Same CDS sequence)	NM_166875.3 , NM_001297842.1 , NM_166876.3 , NM_001272197.1
Accession Version	NM_001272197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259126.1
CDS	1220..4681
Translation	MPGFRGRRVWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_001272197.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant E (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272197.1

ORF Insert Sequence:

ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG TGCGGTTGCT TCAAGGACGA TGAGCCACCC 
GAGATTTGTG TGGTGGAAGG CGCGTTTACC CTGCAGACGC TCACGCCCAC CCAACCCATG 
CCGTCAGTGG ATGAGCTGGA CACCAAGTTC GCGGAGCTGG TGGAGGAGCT CGATCTGACG 
GCCCCCAACA AGGAGGCGAT GCTCAGCCTG CCCGCGCAGA AGAAATGGCA AATCTACTGC 
TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT GGCCCAGATG CAGCGGCCGT CACCCAGCCA 
CCCACCGCAG AGCACTACAT TGAGCGACTG AAGGAGCTGG TGGTGCACAT ATCGCTCTCG 
CCGGAAGACT CGCCCAGCCA TGAGCTGGGC AATCGATTGG ATGGCCATGC CGCCTTTGTG 
GACGCTTTGA AGACGGCGCT ACGCACATCC ACCCACAGTT TTGTTCTCCG ATTCGTGGAG 
CTGGACGGGC TTCCTGCTCT GCTCGACCTC CTGCTGCAGC TGGACATTCG AGTGGCTAAC 
AGCCCGCTGC ACACCAGTCT CATTGGTTGC ATCAAGGCCC TGATGAACAA CTCGATGGGT 
CGGGCGCATG TGCTAGCCCA TCCAACGGCC ATTGACACCA TAGCCAGATC CCTAGCGGCG 
GACAACATTC GCACGAAAAT CGCCGCCCTG GAGATTCTGG GTGCCGTGTG TCTGGTGCCC 
GGCGGTCATC GCAAGGTGCT GCAGGCCATG CTCCACTTCC AGGTCTTCGC TACGGAACGC 
ACCCGTTTCC AGAGCATTGT CAACGACCTG GACCGCTCGA CCTATGCGTA CAGGGACAAT 
GTCAATCTAA AGACCGCCCT TATGTCATTC GTAAATGCGG TGCTTAATTA CGGACCTGGA 
CAGGAGAACC TCGAGTTCCG ACTGCACTTG AGGTACGAGT TTCTCATGCT CGGTATACAG 
CCTGTGATCG ACAAGCTGCG CACGCACGAG AACGAAACGC TGGACAGGCA TTTGGACTTC 
TTTGAGATGG TTCGTGCTGA GGATGAGAAG GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC 
GTGGACACCA AGAGCGCCGG CTCAATGTTT GAGCTGCTGC GCCGCAAGCT CAGTCATTCG 
CCCGCGTATC CCCACATGCT CTCCCTTCTG CAGCACATGC TTCTGCTGCC CTATACGGGC 
CACTGCACGG AGCACTGGTT GCTGATTGAT CGCGTGGTAC AGCAGATAGT GCTGCAGGTG 
GAACAGCGGC CAAACAGTGA TCTTATCGTC GACCCCGATG ACCCTGAGAA GCAGCTGAAG 
CTAGCCGCCG AGTCGCCAGT CCATGATCCC GACGTGGCGC CCTTGCAGAT CGACGTGGCT 
AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA CAGCTGACGC AAGCGCGAAA GCGGGCTGAC 
GAGCTGGAGC GCGAGAACTT TGACGTGCAG TCGCGGCTGG CCAAGAAGGA GCAGGAACTC 
GATCTCCGCA TGCAAGAGAA AGAGGACTTG GAGACGGGAC TAGCGCGCAT GCGTGAGCGT 
TTGGAGAAGG AGTCCGCGCA GCACTCACAG GCGGTGCAGC GGGCGCAGAC TGCAGAGATG 
CGAGCAGAAG ACCTGCAGCA CCGATTGATC AGCGAGCAGC AGGAACGGGC TCGCTTGGAG 
AGACTGGTTA CCGAGGGCAG CATCCCAGAC GACCAGAAGG TGGCCGGACT CACGGGATGC 
AATGGTGCCG TCTCGCCTCC ACCGGCACCT CCCATGCTTA AGGCCATCCC GCCGCCTCCG 
CCACCCATGG CGCCGTCCAT GATGCCCCCA CCACCACCTC CTTGCCCGGG AGCTCCTCCA 
CCTCCGCCAA GCATGGCACA AACGATGGCT CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG 
AAGAATGTGC CACAGCCGAC AAATCCCCTG AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC 
GCCAAGCTGC AGGGTACCGT TTGGAGCGAG CTCGACGAAA GCAAGCTGTA CAACAACATG 
GAGCTGGAGT CAATTGATAA ACTTTTCTCG GCTTACCAAA AGAACGGGGT ATCGGCCACT 
GATGGATCCT ATGAGGACTT ACGGGTAACT GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG 
GTGATCGACG GACGTCGGGC GCAGAACTGC ACCATTCTGC TGAGCAAACT GAAGATGAGC 
GACATGGAGA TATCAAAGGC CATTCTTTCC ATGGACAGCA ACGAGCAGCT GCAGCTGGAC 
ATGGTCGAGC AGCTGCTCAA GTTTACGCCC TCGGCGGAGG AGCGAGCTTT GCTGGACGAG 
CACAGCGAGG ACATTGAGTC TCTCGCTCGG GCCGATCGAT TCCTCTATGA GATATCCAAG 
ATTCCGCACT ACGAGCAGCG ACTGAAGAGT CTGCACTACA AGAAGCGTTT CATGCTGACC 
ATCAACGACC TGGTCCCCCG CATAACCAGT GTGATGGAGG CCTCTCGTGA GGTGGCTCGT 
TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG GTCCTCGCCC TAGGCAACTA CATGAACCGC 
GGGGCACGTG GCAACGCATC GGGATTCCGA CTGGCGTCAC TCAACCGGCT GGCGGACACC 
AAGTCCAGTG CCGCCAAGGG CACCACCCTG CTACACTACC TCGTGCAGGT GATTGAACGC 
AAATTCAAGG ACCTTCTTAA GTTGGAGGAC GATATCCCGC ATGTGCGCGA AGCCTCCAAG 
GTGTCGCTGG GCGAGATGGA CAAGGACATT CAGATGCTGC GCACTGGTCT GGCAGACGTA 
GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG GGCCCGGCCC AACAGGGTGA CCGCTTTCTG 
CCCGTAATGC GCGAGTTCCA CGCCCAGGCG TCAGTGCGCT TCGCTGAGCT GGAGGACAAG 
TTCCAGGACA TGAAGACGCG CTTTGACCGT GCTGTACGCC TCTTCGGCGA GGATGGTTCG 
GTTTTGCAGC CGGACGAGTT CTTTGGCATC TTTGACTCCT TCCTCGCCGC CTTCGCGGAG 
GCGCGGCATG ACAACGAGAG CTTCCGTCGG CGACAGGAGG AAGAGGAGAA GCGTGCCAAG 
CAGGAGGCGG AGCTCAAAAA GCGCACAATA GAGCGCAAGA ACAAGACCGG CCTAATGACC 
AGCGTGGCTC GCAATCTGGG CCTCAAGTCA GGCTCTTCCA ACGGGGATCC TGACTCCCCG 
GCAAAGGGCG GCGACAACAA GGGCGAGTTT GACGATCTCA TCTCGGCCCT GAGGACCGGC 
GACGTGTTTG GCGAGGACAT GGCCAAGTTC AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC 
GGCGGCGGAT CCTCCACTGG GCACACCTCG CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG 
GAGAGTGGGC GTGAGCGCGA GCGGACCGTG AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM ( NM_130544.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569900.3 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant A (DAAM), mRNA.

Price & Availability↑

CloneID	OFa00452
Clone ID Related Accession (Same CDS sequence)	NM_130544.4
Accession Version	NM_130544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_130544.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGTCAG TGGATGAGCT GGACACCAAG TTCGCGGAGC TGGTGGAGGA GCTCGATCTG 
ACGGCCCCCA ACAAGGAGGC GATGCTCAGC CTGCCCGCGC AGAAGAAATG GCAAATCTAC 
TGCTCGAGAA AGCTGCCCTT GGATGCGGCC GATGGCCCAG ATGCAGCGGC CGTCACCCAG 
CCACCCACCG CAGAGCACTA CATTGAGCGA CTGAAGGAGC TGGTGGTGCA CATATCGCTC 
TCGCCGGAAG ACTCGCCCAG CCATGAGCTG GGCAATCGAT TGGATGGCCA TGCCGCCTTT 
GTGGACGCTT TGAAGACGGC GCTACGCACA TCCACCCACA GTTTTGTTCT CCGATTCGTG 
GAGCTGGACG GGCTTCCTGC TCTGCTCGAC CTCCTGCTGC AGCTGGACAT TCGAGTGGCT 
AACAGCCCGC TGCACACCAG TCTCATTGGT TGCATCAAGG CCCTGATGAA CAACTCGATG 
GGTCGGGCGC ATGTGCTAGC CCATCCAACG GCCATTGACA CCATAGCCAG ATCCCTAGCG 
GCGGACAACA TTCGCACGAA AATCGCCGCC CTGGAGATTC TGGGTGCCGT GTGTCTGGTG 
CCCGGCGGTC ATCGCAAGGT GCTGCAGGCC ATGCTCCACT TCCAGGTCTT CGCTACGGAA 
CGCACCCGTT TCCAGAGCAT TGTCAACGAC CTGGACCGCT CGACCTATGC GTACAGGGAC 
AATGTCAATC TAAAGACCGC CCTTATGTCA TTCGTAAATG CGGTGCTTAA TTACGGACCT 
GGACAGGAGA ACCTCGAGTT CCGACTGCAC TTGAGGTACG AGTTTCTCAT GCTCGGTATA 
CAGCCTGTGA TCGACAAGCT GCGCACGCAC GAGAACGAAA CGCTGGACAG GCATTTGGAC 
TTCTTTGAGA TGGTTCGTGC TGAGGATGAG AAGGAGTTTG CCCGCCGTTT TAAGGAGGAG 
CACGTGGACA CCAAGAGCGC CGGCTCAATG TTTGAGCTGC TGCGCCGCAA GCTCAGTCAT 
TCGCCCGCGT ATCCCCACAT GCTCTCCCTT CTGCAGCACA TGCTTCTGCT GCCCTATACG 
GGCCACTGCA CGGAGCACTG GTTGCTGATT GATCGCGTGG TACAGCAGAT AGTGCTGCAG 
GTGGAACAGC GGCCAAACAG TGATCTTATC GTCGACCCCG ATGACCCTGA GAAGCAGCTG 
AAGCTAGCCG CCGAGTCGCC AGTCCATGAT CCCGACGTGG CGCCCTTGCA GATCGACGTG 
GCTAAGCTTG TGCGTCTGCT GGTCAAGGAG GAACAGCTGA CGCAAGCGCG AAAGCGGGCT 
GACGAGCTGG AGCGCGAGAA CTTTGACGTG CAGTCGCGGC TGGCCAAGAA GGAGCAGGAA 
CTCGATCTCC GCATGCAAGA GAAAGAGGAC TTGGAGACGG GACTAGCGCG CATGCGTGAG 
CGTTTGGAGA AGGAGTCCGC GCAGCACTCA CAGGCGGTGC AGCGGGCGCA GACTGCAGAG 
ATGCGAGCAG AAGACCTGCA GCACCGATTG ATCAGCGAGC AGCAGGAACG GGCTCGCTTG 
GAGAGACTGG TTACCGAGGG CAGCATCCCA GACGACCAGA AGGTGGCCGG ACTCACGGGA 
TGCAATGGTG CCGTCTCGCC TCCACCGGCA CCTCCCATGC TTAAGGCCAT CCCGCCGCCT 
CCGCCACCCA TGGCGCCGTC CATGATGCCC CCACCACCAC CTCCTTGCCC GGGAGCTCCT 
CCACCTCCGC CAAGCATGGC ACAAACGATG GCTCCAGCGC CACCAAAAGT GGATCTGCCA 
AAGAAGAATG TGCCACAGCC GACAAATCCC CTGAAGAGCT TCAACTGGTC GAAACTGCCG 
GACGCCAAGC TGCAGGGTAC CGTTTGGAGC GAGCTCGACG AAAGCAAGCT GTACAACAAC 
ATGGAGCTGG AGTCAATTGA TAAACTTTTC TCGGCTTACC AAAAGAACGG GGTATCGGCC 
ACTGATGGAT CCTATGAGGA CTTACGGGTA ACTGGCAAGG CTGCCAAGCA GAAAGTGCTG 
TCGGTGATCG ACGGACGTCG GGCGCAGAAC TGCACCATTC TGCTGAGCAA ACTGAAGATG 
AGCGACATGG AGATATCAAA GGCCATTCTT TCCATGGACA GCAACGAGCA GCTGCAGCTG 
GACATGGTCG AGCAGCTGCT CAAGTTTACG CCCTCGGCGG AGGAGCGAGC TTTGCTGGAC 
GAGCACAGCG AGGACATTGA GTCTCTCGCT CGGGCCGATC GATTCCTCTA TGAGATATCC 
AAGATTCCGC ACTACGAGCA GCGACTGAAG AGTCTGCACT ACAAGAAGCG TTTCATGCTG 
ACCATCAACG ACCTGGTCCC CCGCATAACC AGTGTGATGG AGGCCTCTCG TGAGGTGGCT 
CGTTCCCGTC GCCTGCGTAA GCTTCTGGAG TTGGTCCTCG CCCTAGGCAA CTACATGAAC 
CGCGGGGCAC GTGGCAACGC ATCGGGATTC CGACTGGCGT CACTCAACCG GCTGGCGGAC 
ACCAAGTCCA GTGCCGCCAA GGGCACCACC CTGCTACACT ACCTCGTGCA GGTGATTGAA 
CGCAAATTCA AGGACCTTCT TAAGTTGGAG GACGATATCC CGCATGTGCG CGAAGCCTCC 
AAGGTGTCGC TGGGCGAGAT GGACAAGGAC ATTCAGATGC TGCGCACTGG TCTGGCAGAC 
GTAGCGCGCG AAATCGAGTT CCACCGCAGT TCGGGCCCGG CCCAACAGGG TGACCGCTTT 
CTGCCCGTAA TGCGCGAGTT CCACGCCCAG GCGTCAGTGC GCTTCGCTGA GCTGGAGGAC 
AAGTTCCAGG ACATGAAGAC GCGCTTTGAC CGTGCTGTAC GCCTCTTCGG CGAGGATGGT 
TCGGTTTTGC AGCCGGACGA GTTCTTTGGC ATCTTTGACT CCTTCCTCGC CGCCTTCGCG 
GAGGCGCGGC ATGACAACGA GAGCTTCCGT CGGCGACAGG AGGAAGAGGA GAAGCGTGCC 
AAGCAGGAGG CGGAGCTCAA AAAGCGCACA ATAGAGCGCA AGAACAAGAC CGGCCTAATG 
ACCAGCGTGG CTCGCAATCT GGGCCTCAAG TCAGGCTCTT CCAACGGGGA TCCTGACTCC 
CCGGCAAAGG GCGGCGACAA CAAGGGCGAG TTTGACGATC TCATCTCGGC CCTGAGGACC 
GGCGACGTGT TTGGCGAGGA CATGGCCAAG TTCAAGCGGT CGCGCAAGGC GCGCGTGCTT 
AACGGCGGCG GATCCTCCAC TGGGCACACC TCGCCGCCCC GCCACGGCAG CCTCCAAAGG 
GAGGAGAGTG GGCGTGAGCG CGAGCGGACC GTGAGGCGCC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569900.3
CDS	197..3541
Translation	MPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_130544.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant A (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130544.4

ORF Insert Sequence:

ATGCCGTCAG TGGATGAGCT GGACACCAAG TTCGCGGAGC TGGTGGAGGA GCTCGATCTG 
ACGGCCCCCA ACAAGGAGGC GATGCTCAGC CTGCCCGCGC AGAAGAAATG GCAAATCTAC 
TGCTCGAGAA AGCTGCCCTT GGATGCGGCC GATGGCCCAG ATGCAGCGGC CGTCACCCAG 
CCACCCACCG CAGAGCACTA CATTGAGCGA CTGAAGGAGC TGGTGGTGCA CATATCGCTC 
TCGCCGGAAG ACTCGCCCAG CCATGAGCTG GGCAATCGAT TGGATGGCCA TGCCGCCTTT 
GTGGACGCTT TGAAGACGGC GCTACGCACA TCCACCCACA GTTTTGTTCT CCGATTCGTG 
GAGCTGGACG GGCTTCCTGC TCTGCTCGAC CTCCTGCTGC AGCTGGACAT TCGAGTGGCT 
AACAGCCCGC TGCACACCAG TCTCATTGGT TGCATCAAGG CCCTGATGAA CAACTCGATG 
GGTCGGGCGC ATGTGCTAGC CCATCCAACG GCCATTGACA CCATAGCCAG ATCCCTAGCG 
GCGGACAACA TTCGCACGAA AATCGCCGCC CTGGAGATTC TGGGTGCCGT GTGTCTGGTG 
CCCGGCGGTC ATCGCAAGGT GCTGCAGGCC ATGCTCCACT TCCAGGTCTT CGCTACGGAA 
CGCACCCGTT TCCAGAGCAT TGTCAACGAC CTGGACCGCT CGACCTATGC GTACAGGGAC 
AATGTCAATC TAAAGACCGC CCTTATGTCA TTCGTAAATG CGGTGCTTAA TTACGGACCT 
GGACAGGAGA ACCTCGAGTT CCGACTGCAC TTGAGGTACG AGTTTCTCAT GCTCGGTATA 
CAGCCTGTGA TCGACAAGCT GCGCACGCAC GAGAACGAAA CGCTGGACAG GCATTTGGAC 
TTCTTTGAGA TGGTTCGTGC TGAGGATGAG AAGGAGTTTG CCCGCCGTTT TAAGGAGGAG 
CACGTGGACA CCAAGAGCGC CGGCTCAATG TTTGAGCTGC TGCGCCGCAA GCTCAGTCAT 
TCGCCCGCGT ATCCCCACAT GCTCTCCCTT CTGCAGCACA TGCTTCTGCT GCCCTATACG 
GGCCACTGCA CGGAGCACTG GTTGCTGATT GATCGCGTGG TACAGCAGAT AGTGCTGCAG 
GTGGAACAGC GGCCAAACAG TGATCTTATC GTCGACCCCG ATGACCCTGA GAAGCAGCTG 
AAGCTAGCCG CCGAGTCGCC AGTCCATGAT CCCGACGTGG CGCCCTTGCA GATCGACGTG 
GCTAAGCTTG TGCGTCTGCT GGTCAAGGAG GAACAGCTGA CGCAAGCGCG AAAGCGGGCT 
GACGAGCTGG AGCGCGAGAA CTTTGACGTG CAGTCGCGGC TGGCCAAGAA GGAGCAGGAA 
CTCGATCTCC GCATGCAAGA GAAAGAGGAC TTGGAGACGG GACTAGCGCG CATGCGTGAG 
CGTTTGGAGA AGGAGTCCGC GCAGCACTCA CAGGCGGTGC AGCGGGCGCA GACTGCAGAG 
ATGCGAGCAG AAGACCTGCA GCACCGATTG ATCAGCGAGC AGCAGGAACG GGCTCGCTTG 
GAGAGACTGG TTACCGAGGG CAGCATCCCA GACGACCAGA AGGTGGCCGG ACTCACGGGA 
TGCAATGGTG CCGTCTCGCC TCCACCGGCA CCTCCCATGC TTAAGGCCAT CCCGCCGCCT 
CCGCCACCCA TGGCGCCGTC CATGATGCCC CCACCACCAC CTCCTTGCCC GGGAGCTCCT 
CCACCTCCGC CAAGCATGGC ACAAACGATG GCTCCAGCGC CACCAAAAGT GGATCTGCCA 
AAGAAGAATG TGCCACAGCC GACAAATCCC CTGAAGAGCT TCAACTGGTC GAAACTGCCG 
GACGCCAAGC TGCAGGGTAC CGTTTGGAGC GAGCTCGACG AAAGCAAGCT GTACAACAAC 
ATGGAGCTGG AGTCAATTGA TAAACTTTTC TCGGCTTACC AAAAGAACGG GGTATCGGCC 
ACTGATGGAT CCTATGAGGA CTTACGGGTA ACTGGCAAGG CTGCCAAGCA GAAAGTGCTG 
TCGGTGATCG ACGGACGTCG GGCGCAGAAC TGCACCATTC TGCTGAGCAA ACTGAAGATG 
AGCGACATGG AGATATCAAA GGCCATTCTT TCCATGGACA GCAACGAGCA GCTGCAGCTG 
GACATGGTCG AGCAGCTGCT CAAGTTTACG CCCTCGGCGG AGGAGCGAGC TTTGCTGGAC 
GAGCACAGCG AGGACATTGA GTCTCTCGCT CGGGCCGATC GATTCCTCTA TGAGATATCC 
AAGATTCCGC ACTACGAGCA GCGACTGAAG AGTCTGCACT ACAAGAAGCG TTTCATGCTG 
ACCATCAACG ACCTGGTCCC CCGCATAACC AGTGTGATGG AGGCCTCTCG TGAGGTGGCT 
CGTTCCCGTC GCCTGCGTAA GCTTCTGGAG TTGGTCCTCG CCCTAGGCAA CTACATGAAC 
CGCGGGGCAC GTGGCAACGC ATCGGGATTC CGACTGGCGT CACTCAACCG GCTGGCGGAC 
ACCAAGTCCA GTGCCGCCAA GGGCACCACC CTGCTACACT ACCTCGTGCA GGTGATTGAA 
CGCAAATTCA AGGACCTTCT TAAGTTGGAG GACGATATCC CGCATGTGCG CGAAGCCTCC 
AAGGTGTCGC TGGGCGAGAT GGACAAGGAC ATTCAGATGC TGCGCACTGG TCTGGCAGAC 
GTAGCGCGCG AAATCGAGTT CCACCGCAGT TCGGGCCCGG CCCAACAGGG TGACCGCTTT 
CTGCCCGTAA TGCGCGAGTT CCACGCCCAG GCGTCAGTGC GCTTCGCTGA GCTGGAGGAC 
AAGTTCCAGG ACATGAAGAC GCGCTTTGAC CGTGCTGTAC GCCTCTTCGG CGAGGATGGT 
TCGGTTTTGC AGCCGGACGA GTTCTTTGGC ATCTTTGACT CCTTCCTCGC CGCCTTCGCG 
GAGGCGCGGC ATGACAACGA GAGCTTCCGT CGGCGACAGG AGGAAGAGGA GAAGCGTGCC 
AAGCAGGAGG CGGAGCTCAA AAAGCGCACA ATAGAGCGCA AGAACAAGAC CGGCCTAATG 
ACCAGCGTGG CTCGCAATCT GGGCCTCAAG TCAGGCTCTT CCAACGGGGA TCCTGACTCC 
CCGGCAAAGG GCGGCGACAA CAAGGGCGAG TTTGACGATC TCATCTCGGC CCTGAGGACC 
GGCGACGTGT TTGGCGAGGA CATGGCCAAG TTCAAGCGGT CGCGCAAGGC GCGCGTGCTT 
AACGGCGGCG GATCCTCCAC TGGGCACACC TCGCCGCCCC GCCACGGCAG CCTCCAAAGG 
GAGGAGAGTG GGCGTGAGCG CGAGCGGACC GTGAGGCGCC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM ( NM_001201593.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188522.1 Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant D (DAAM), mRNA.

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CloneID	OFa02960
Clone ID Related Accession (Same CDS sequence)	NM_001201593.2
Accession Version	NM_001201593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled associated activator of morphogenesis, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001201593.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCA TGAATCAAAT GAGTGTCCTC ATGGATAAGA TCAACACGAC TCTACAGTCG 
TGTCCCATCG TGTGCATCAA TGAGCCGGAC ACACACAGTG CAAACACAGA GATATTGCAG 
ACAAGTCCGG AGAGCACGCT AGAGAAGCCC AAGGCGGCGC CATCCGGTGG AGCCAGCACG 
AGTGTGACTA GCTTGAACGG CAGCTGCATT GCCATCAATG GAGGAATAGG AGAAGGAGCC 
ACTGCAACTG CATCGGCAGC TACCAGCACT GCGGCTGGAG TGACTAAGAT AACGATAACC 
AGTGACAACG GCAACGGCAA CGCCAGCACC AGCACCAAAG CACCAACCAC AGGCAGTTCC 
ATCGACGGCT ACAAGAACAG CAGTTTGGTG TCCATCACCA GCCTGAACAG CTGCAGCGAC 
CTAAGCCAGG GCAGCACCGC CACCCTGCCC ATCAGCAGCA ACAGCTGCAG CGGCAACAAC 
GTCAGCAGGC ATCCCCTCAG CAACAACAAC AGCGGCAGCG ACACCTCGTC CAACAACTTC 
CAGGAGCGAT TCGACTTTGA ACACTGGAAG GGCTTACTCA GCGACTACAA CTGCTTCCAC 
GGGCTGTATC GCTACTGGAC TCAGTGCAGT GGACGCAGCA GCAGCGCCAA CGACAACGGC 
GGTGGCAGTG ATCAGCAGCA GTCGCAGTCC AACCATGAGA ACGGCCTGGG CAGCGAAGCC 
GGTTCCTTCT ACACCCACAA TATACTAGGC TACACCTTCG GCTATCCGTT TGGCCTAAAG 
GAGGATCTCG ACGGCAGCGG CAATTGCAAC AGCAACTGCA ACGGCAGCAG CAACACCAAC 
GGTCTTGGCC TCAGGAAGTG GCTTTCAGGC AATACCAGCA GCAACGAGAC GCCGCTACAG 
AAGCACTACA GACACCAGCA GAAGAAGAAG ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG 
TGCGGTTGCT TCAAGGACGA TGAGCCACCC GAGATTTGTG TGGTGGAAGG CGCGTTTACC 
CTGCAGACGC TCACGCCCAC CCAACCCATG CCGTCAGTGG ATGAGCTGGA CACCAAGTTC 
GCGGAGCTGG TGGAGGAGCT CGATCTGACG GCCCCCAACA AGGAGGCGAT GCTCAGCCTG 
CCCGCGCAGA AGAAATGGCA AATCTACTGC TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT 
GGCCCAGATG CAGCGGCCGT CACCCAGCCA CCCACCGCAG AGCACTACAT TGAGCGACTG 
AAGGAGCTGG TGGTGCACAT ATCGCTCTCG CCGGAAGACT CGCCCAGCCA TGAGCTGGGC 
AATCGATTGG ATGGCCATGC CGCCTTTGTG GACGCTTTGA AGACGGCGCT ACGCACATCC 
ACCCACAGTT TTGTTCTCCG ATTCGTGGAG CTGGACGGGC TTCCTGCTCT GCTCGACCTC 
CTGCTGCAGC TGGACATTCG AGTGGCTAAC AGCCCGCTGC ACACCAGTCT CATTGGTTGC 
ATCAAGGCCC TGATGAACAA CTCGATGGGT CGGGCGCATG TGCTAGCCCA TCCAACGGCC 
ATTGACACCA TAGCCAGATC CCTAGCGGCG GACAACATTC GCACGAAAAT CGCCGCCCTG 
GAGATTCTGG GTGCCGTGTG TCTGGTGCCC GGCGGTCATC GCAAGGTGCT GCAGGCCATG 
CTCCACTTCC AGGTCTTCGC TACGGAACGC ACCCGTTTCC AGAGCATTGT CAACGACCTG 
GACCGCTCGA CCTATGCGTA CAGGGACAAT GTCAATCTAA AGACCGCCCT TATGTCATTC 
GTAAATGCGG TGCTTAATTA CGGACCTGGA CAGGAGAACC TCGAGTTCCG ACTGCACTTG 
AGGTACGAGT TTCTCATGCT CGGTATACAG CCTGTGATCG ACAAGCTGCG CACGCACGAG 
AACGAAACGC TGGACAGGCA TTTGGACTTC TTTGAGATGG TTCGTGCTGA GGATGAGAAG 
GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC GTGGACACCA AGAGCGCCGG CTCAATGTTT 
GAGCTGCTGC GCCGCAAGCT CAGTCATTCG CCCGCGTATC CCCACATGCT CTCCCTTCTG 
CAGCACATGC TTCTGCTGCC CTATACGGGC CACTGCACGG AGCACTGGTT GCTGATTGAT 
CGCGTGGTAC AGCAGATAGT GCTGCAGGTG GAACAGCGGC CAAACAGTGA TCTTATCGTC 
GACCCCGATG ACCCTGAGAA GCAGCTGAAG CTAGCCGCCG AGTCGCCAGT CCATGATCCC 
GACGTGGCGC CCTTGCAGAT CGACGTGGCT AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA 
CAGCTGACGC AAGCGCGAAA GCGGGCTGAC GAGCTGGAGC GCGAGAACTT TGACGTGCAG 
TCGCGGCTGG CCAAGAAGGA GCAGGAACTC GATCTCCGCA TGCAAGAGAA AGAGGACTTG 
GAGACGGGAC TAGCGCGCAT GCGTGAGCGT TTGGAGAAGG AGTCCGCGCA GCACTCACAG 
GCGGTGCAGC GGGCGCAGAC TGCAGAGATG CGAGCAGAAG ACCTGCAGCA CCGATTGATC 
AGCGAGCAGC AGGAACGGGC TCGCTTGGAG AGACTGGTTA CCGAGGGCAG CATCCCAGAC 
GACCAGAAGG TGGCCGGACT CACGGGATGC AATGGTGCCG TCTCGCCTCC ACCGGCACCT 
CCCATGCTTA AGGCCATCCC GCCGCCTCCG CCACCCATGG CGCCGTCCAT GATGCCCCCA 
CCACCACCTC CTTGCCCGGG AGCTCCTCCA CCTCCGCCAA GCATGGCACA AACGATGGCT 
CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG AAGAATGTGC CACAGCCGAC AAATCCCCTG 
AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC GCCAAGCTGC AGGGTACCGT TTGGAGCGAG 
CTCGACGAAA GCAAGCTGTA CAACAACATG GAGCTGGAGT CAATTGATAA ACTTTTCTCG 
GCTTACCAAA AGAACGGGGT ATCGGCCACT GATGGATCCT ATGAGGACTT ACGGGTAACT 
GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG GTGATCGACG GACGTCGGGC GCAGAACTGC 
ACCATTCTGC TGAGCAAACT GAAGATGAGC GACATGGAGA TATCAAAGGC CATTCTTTCC 
ATGGACAGCA ACGAGCAGCT GCAGCTGGAC ATGGTCGAGC AGCTGCTCAA GTTTACGCCC 
TCGGCGGAGG AGCGAGCTTT GCTGGACGAG CACAGCGAGG ACATTGAGTC TCTCGCTCGG 
GCCGATCGAT TCCTCTATGA GATATCCAAG ATTCCGCACT ACGAGCAGCG ACTGAAGAGT 
CTGCACTACA AGAAGCGTTT CATGCTGACC ATCAACGACC TGGTCCCCCG CATAACCAGT 
GTGATGGAGG CCTCTCGTGA GGTGGCTCGT TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG 
GTCCTCGCCC TAGGCAACTA CATGAACCGC GGGGCACGTG GCAACGCATC GGGATTCCGA 
CTGGCGTCAC TCAACCGGCT GGCGGACACC AAGTCCAGTG CCGCCAAGGG CACCACCCTG 
CTACACTACC TCGTGCAGGT GATTGAACGC AAATTCAAGG ACCTTCTTAA GTTGGAGGAC 
GATATCCCGC ATGTGCGCGA AGCCTCCAAG GTGTCGCTGG GCGAGATGGA CAAGGACATT 
CAGATGCTGC GCACTGGTCT GGCAGACGTA GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG 
GGCCCGGCCC AACAGGGTGA CCGCTTTCTG CCCGTAATGC GCGAGTTCCA CGCCCAGGCG 
TCAGTGCGCT TCGCTGAGCT GGAGGACAAG TTCCAGGACA TGAAGACGCG CTTTGACCGT 
GCTGTACGCC TCTTCGGCGA GGATGGTTCG GTTTTGCAGC CGGACGAGTT CTTTGGCATC 
TTTGACTCCT TCCTCGCCGC CTTCGCGGAG GCGCGGCATG ACAACGAGAG CTTCCGTCGG 
CGACAGGAGG AAGAGGAGAA GCGTGCCAAG CAGGAGGCGG AGCTCAAAAA GCGCACAATA 
GAGCGCAAGA ACAAGACCGG CCTAATGACC AGCGTGGCTC GCAATCTGGG CCTCAAGTCA 
GGCTCTTCCA ACGGGGATCC TGACTCCCCG GCAAAGGGCG GCGACAACAA GGGCGAGTTT 
GACGATCTCA TCTCGGCCCT GAGGACCGGC GACGTGTTTG GCGAGGACAT GGCCAAGTTC 
AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC GGCGGCGGAT CCTCCACTGG GCACACCTCG 
CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG GAGAGTGGGC GTGAGCGCGA GCGGACCGTG 
AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188522.1
CDS	350..4741
Translation	MSSMNQMSVLMDKINTTLQSCPIVCINEPDTHSANTEILQTSPESTLEKPKAAPSGGASTSVTSLNGSCIAINGGIGEGATATASAATSTAAGVTKITITSDNGNGNASTSTKAPTTGSSIDGYKNSSLVSITSLNSCSDLSQGSTATLPISSNSCSGNNVSRHPLSNNNSGSDTSSNNFQERFDFEHWKGLLSDYNCFHGLYRYWTQCSGRSSSANDNGGGSDQQQSQSNHENGLGSEAGSFYTHNILGYTFGYPFGLKEDLDGSGNCNSNCNGSSNTNGLGLRKWLSGNTSSNETPLQKHYRHQQKKKMPGFRGRRVWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKLPLDAADGPDAAAVTQPPTAEHYIERLKELVVHISLSPEDSPSHELGNRLDGHAAFVDALKTALRTSTHSFVLRFVELDGLPALLDLLLQLDIRVANSPLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQVFATERTRFQSIVNDLDRSTYAYRDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFKEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPNSDLIVDPDDPEKQLKLAAESPVHDPDVAPLQIDVAKLVRLLVKEEQLTQARKRADELERENFDVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQTAEMRAEDLQHRLISEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPAPPMLKAIPPPPPPMAPSMMPPPPPPCPGAPPPPPSMAQTMAPAPPKVDLPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVSATDGSYEDLRVTGKAAKQKVLSVIDGRRAQNCTILLSKLKMSDMEISKAILSMDSNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTINDLVPRITSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIERKFKDLLKLEDDIPHVREASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLAAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIERKNKTGLMTSVARNLGLKSGSSNGDPDSPAKGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGGSSTGHTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	NM_001201593.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled associated activator of morphogenesis, transcript variant D (DAAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201593.2

ORF Insert Sequence:

ATGTCGAGCA TGAATCAAAT GAGTGTCCTC ATGGATAAGA TCAACACGAC TCTACAGTCG 
TGTCCCATCG TGTGCATCAA TGAGCCGGAC ACACACAGTG CAAACACAGA GATATTGCAG 
ACAAGTCCGG AGAGCACGCT AGAGAAGCCC AAGGCGGCGC CATCCGGTGG AGCCAGCACG 
AGTGTGACTA GCTTGAACGG CAGCTGCATT GCCATCAATG GAGGAATAGG AGAAGGAGCC 
ACTGCAACTG CATCGGCAGC TACCAGCACT GCGGCTGGAG TGACTAAGAT AACGATAACC 
AGTGACAACG GCAACGGCAA CGCCAGCACC AGCACCAAAG CACCAACCAC AGGCAGTTCC 
ATCGACGGCT ACAAGAACAG CAGTTTGGTG TCCATCACCA GCCTGAACAG CTGCAGCGAC 
CTAAGCCAGG GCAGCACCGC CACCCTGCCC ATCAGCAGCA ACAGCTGCAG CGGCAACAAC 
GTCAGCAGGC ATCCCCTCAG CAACAACAAC AGCGGCAGCG ACACCTCGTC CAACAACTTC 
CAGGAGCGAT TCGACTTTGA ACACTGGAAG GGCTTACTCA GCGACTACAA CTGCTTCCAC 
GGGCTGTATC GCTACTGGAC TCAGTGCAGT GGACGCAGCA GCAGCGCCAA CGACAACGGC 
GGTGGCAGTG ATCAGCAGCA GTCGCAGTCC AACCATGAGA ACGGCCTGGG CAGCGAAGCC 
GGTTCCTTCT ACACCCACAA TATACTAGGC TACACCTTCG GCTATCCGTT TGGCCTAAAG 
GAGGATCTCG ACGGCAGCGG CAATTGCAAC AGCAACTGCA ACGGCAGCAG CAACACCAAC 
GGTCTTGGCC TCAGGAAGTG GCTTTCAGGC AATACCAGCA GCAACGAGAC GCCGCTACAG 
AAGCACTACA GACACCAGCA GAAGAAGAAG ATGCCCGGCT TCAGGGGCAG AAGGGTGTGG 
TGCGGTTGCT TCAAGGACGA TGAGCCACCC GAGATTTGTG TGGTGGAAGG CGCGTTTACC 
CTGCAGACGC TCACGCCCAC CCAACCCATG CCGTCAGTGG ATGAGCTGGA CACCAAGTTC 
GCGGAGCTGG TGGAGGAGCT CGATCTGACG GCCCCCAACA AGGAGGCGAT GCTCAGCCTG 
CCCGCGCAGA AGAAATGGCA AATCTACTGC TCGAGAAAGC TGCCCTTGGA TGCGGCCGAT 
GGCCCAGATG CAGCGGCCGT CACCCAGCCA CCCACCGCAG AGCACTACAT TGAGCGACTG 
AAGGAGCTGG TGGTGCACAT ATCGCTCTCG CCGGAAGACT CGCCCAGCCA TGAGCTGGGC 
AATCGATTGG ATGGCCATGC CGCCTTTGTG GACGCTTTGA AGACGGCGCT ACGCACATCC 
ACCCACAGTT TTGTTCTCCG ATTCGTGGAG CTGGACGGGC TTCCTGCTCT GCTCGACCTC 
CTGCTGCAGC TGGACATTCG AGTGGCTAAC AGCCCGCTGC ACACCAGTCT CATTGGTTGC 
ATCAAGGCCC TGATGAACAA CTCGATGGGT CGGGCGCATG TGCTAGCCCA TCCAACGGCC 
ATTGACACCA TAGCCAGATC CCTAGCGGCG GACAACATTC GCACGAAAAT CGCCGCCCTG 
GAGATTCTGG GTGCCGTGTG TCTGGTGCCC GGCGGTCATC GCAAGGTGCT GCAGGCCATG 
CTCCACTTCC AGGTCTTCGC TACGGAACGC ACCCGTTTCC AGAGCATTGT CAACGACCTG 
GACCGCTCGA CCTATGCGTA CAGGGACAAT GTCAATCTAA AGACCGCCCT TATGTCATTC 
GTAAATGCGG TGCTTAATTA CGGACCTGGA CAGGAGAACC TCGAGTTCCG ACTGCACTTG 
AGGTACGAGT TTCTCATGCT CGGTATACAG CCTGTGATCG ACAAGCTGCG CACGCACGAG 
AACGAAACGC TGGACAGGCA TTTGGACTTC TTTGAGATGG TTCGTGCTGA GGATGAGAAG 
GAGTTTGCCC GCCGTTTTAA GGAGGAGCAC GTGGACACCA AGAGCGCCGG CTCAATGTTT 
GAGCTGCTGC GCCGCAAGCT CAGTCATTCG CCCGCGTATC CCCACATGCT CTCCCTTCTG 
CAGCACATGC TTCTGCTGCC CTATACGGGC CACTGCACGG AGCACTGGTT GCTGATTGAT 
CGCGTGGTAC AGCAGATAGT GCTGCAGGTG GAACAGCGGC CAAACAGTGA TCTTATCGTC 
GACCCCGATG ACCCTGAGAA GCAGCTGAAG CTAGCCGCCG AGTCGCCAGT CCATGATCCC 
GACGTGGCGC CCTTGCAGAT CGACGTGGCT AAGCTTGTGC GTCTGCTGGT CAAGGAGGAA 
CAGCTGACGC AAGCGCGAAA GCGGGCTGAC GAGCTGGAGC GCGAGAACTT TGACGTGCAG 
TCGCGGCTGG CCAAGAAGGA GCAGGAACTC GATCTCCGCA TGCAAGAGAA AGAGGACTTG 
GAGACGGGAC TAGCGCGCAT GCGTGAGCGT TTGGAGAAGG AGTCCGCGCA GCACTCACAG 
GCGGTGCAGC GGGCGCAGAC TGCAGAGATG CGAGCAGAAG ACCTGCAGCA CCGATTGATC 
AGCGAGCAGC AGGAACGGGC TCGCTTGGAG AGACTGGTTA CCGAGGGCAG CATCCCAGAC 
GACCAGAAGG TGGCCGGACT CACGGGATGC AATGGTGCCG TCTCGCCTCC ACCGGCACCT 
CCCATGCTTA AGGCCATCCC GCCGCCTCCG CCACCCATGG CGCCGTCCAT GATGCCCCCA 
CCACCACCTC CTTGCCCGGG AGCTCCTCCA CCTCCGCCAA GCATGGCACA AACGATGGCT 
CCAGCGCCAC CAAAAGTGGA TCTGCCAAAG AAGAATGTGC CACAGCCGAC AAATCCCCTG 
AAGAGCTTCA ACTGGTCGAA ACTGCCGGAC GCCAAGCTGC AGGGTACCGT TTGGAGCGAG 
CTCGACGAAA GCAAGCTGTA CAACAACATG GAGCTGGAGT CAATTGATAA ACTTTTCTCG 
GCTTACCAAA AGAACGGGGT ATCGGCCACT GATGGATCCT ATGAGGACTT ACGGGTAACT 
GGCAAGGCTG CCAAGCAGAA AGTGCTGTCG GTGATCGACG GACGTCGGGC GCAGAACTGC 
ACCATTCTGC TGAGCAAACT GAAGATGAGC GACATGGAGA TATCAAAGGC CATTCTTTCC 
ATGGACAGCA ACGAGCAGCT GCAGCTGGAC ATGGTCGAGC AGCTGCTCAA GTTTACGCCC 
TCGGCGGAGG AGCGAGCTTT GCTGGACGAG CACAGCGAGG ACATTGAGTC TCTCGCTCGG 
GCCGATCGAT TCCTCTATGA GATATCCAAG ATTCCGCACT ACGAGCAGCG ACTGAAGAGT 
CTGCACTACA AGAAGCGTTT CATGCTGACC ATCAACGACC TGGTCCCCCG CATAACCAGT 
GTGATGGAGG CCTCTCGTGA GGTGGCTCGT TCCCGTCGCC TGCGTAAGCT TCTGGAGTTG 
GTCCTCGCCC TAGGCAACTA CATGAACCGC GGGGCACGTG GCAACGCATC GGGATTCCGA 
CTGGCGTCAC TCAACCGGCT GGCGGACACC AAGTCCAGTG CCGCCAAGGG CACCACCCTG 
CTACACTACC TCGTGCAGGT GATTGAACGC AAATTCAAGG ACCTTCTTAA GTTGGAGGAC 
GATATCCCGC ATGTGCGCGA AGCCTCCAAG GTGTCGCTGG GCGAGATGGA CAAGGACATT 
CAGATGCTGC GCACTGGTCT GGCAGACGTA GCGCGCGAAA TCGAGTTCCA CCGCAGTTCG 
GGCCCGGCCC AACAGGGTGA CCGCTTTCTG CCCGTAATGC GCGAGTTCCA CGCCCAGGCG 
TCAGTGCGCT TCGCTGAGCT GGAGGACAAG TTCCAGGACA TGAAGACGCG CTTTGACCGT 
GCTGTACGCC TCTTCGGCGA GGATGGTTCG GTTTTGCAGC CGGACGAGTT CTTTGGCATC 
TTTGACTCCT TCCTCGCCGC CTTCGCGGAG GCGCGGCATG ACAACGAGAG CTTCCGTCGG 
CGACAGGAGG AAGAGGAGAA GCGTGCCAAG CAGGAGGCGG AGCTCAAAAA GCGCACAATA 
GAGCGCAAGA ACAAGACCGG CCTAATGACC AGCGTGGCTC GCAATCTGGG CCTCAAGTCA 
GGCTCTTCCA ACGGGGATCC TGACTCCCCG GCAAAGGGCG GCGACAACAA GGGCGAGTTT 
GACGATCTCA TCTCGGCCCT GAGGACCGGC GACGTGTTTG GCGAGGACAT GGCCAAGTTC 
AAGCGGTCGC GCAAGGCGCG CGTGCTTAAC GGCGGCGGAT CCTCCACTGG GCACACCTCG 
CCGCCCCGCC ACGGCAGCCT CCAAAGGGAG GAGAGTGGGC GTGAGCGCGA GCGGACCGTG 
AGGCGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DAAM cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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