Gmcl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gmcl1
Entrez Gene ID	312516
Full Name	germ cell-less, spermatogenesis associated 1
Synonyms	Gcl
General protein information	Preferred Names germ cell-less, spermatogenesis associated 1 Names germ cell-less protein-like 1 germ cell-less homolog 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	This gene encodes a nuclear envelope protein that contains a broad-complex, tramtrack and bric a brac/ Pox virus and Zinc finger (BTB/POZ) protein-protein interaction domain. In mouse, this gene is expressed at high levels in the testis, and null mutant mice exhibit morphologically abnormal sperm that have chromatin condensation defects. These defects result in significantly impaired fertility, demonstrating a role for this gene in male germ cell development in mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006236832.3	XP_006236894.1	germ cell-less protein-like 1 isoform X1
NM_001010956.3	NP_001010956.1	germ cell-less protein-like 1 isoform 2
NM_001033931.2	NP_001029103.1	germ cell-less protein-like 1 isoform 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gcl	NP_724708.1
Caenorhabditis elegans (roundworm)	gcl-1	NP_502585.1
Bos taurus (cattle)	GMCL1	NP_001095453.1
Mus musculus (house mouse)	Gmcl1	NP_035948.3
Gallus gallus (chicken)	GMCL1	XP_423987.4
	GMCL1	XP_002799349.1
Rattus norvegicus (Norway rat)	Gmcl1	NP_001029103.1
Homo sapiens (human)	GMCL1	NP_848526.1
Pan troglodytes (chimpanzee)	GMCL1	XP_001148182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011686	XP_320823.3
Canis lupus familiaris (dog)	GMCL1	XP_538534.2
Danio rerio (zebrafish)	gmcl1	NP_996933.1
Xenopus tropicalis (tropical clawed frog)	gmcl1	NP_001072725.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and multiple deformations during mgcl-1 deficient spermatogenesis leading to reduced fertility.
Maekawa M, Ito C, Toyama Y, Suzuki-Toyota F, Kimura T, Nakano T, Toshimori K
Archives of histology and cytology67(4)335-47(2004 Nov)

Reduced human germ cell-less (HGCL) expression in azoospermic men with severe germinal cell impairment.
Kleiman SE, Yogev L, Gal-Yam EN, Hauser R, Gamzu R, Botchan A, Paz G, Yavetz H, Maymon BB, Schreiber L, Barzilai S, Amariglio N, Rechavi G, Simon AJ
Journal of andrology24(5)670-5(2003 Sep-Oct)

Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less.
Masuhara M, Nagao K, Nishikawa M, Kimura T, Nakano T
Biochemical and biophysical research communications308(4)927-32(2003 Sep)

Mouse germ cell-less as an essential component for nuclear integrity.
Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, Toshimori K, Nakano T
Molecular and cellular biology23(4)1304-15(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gmcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gmcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01353	NM_001010956.3 Latest version!	Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa08750	NM_001033931.2 Latest version!	Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa27437	XM_006236832.3 Latest version!	Rattus norvegicus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gmcl1 ( NM_001010956.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001010956.1 Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa01353
Clone ID Related Accession (Same CDS sequence)	NM_001010956.3
Accession Version	NM_001010956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-15
Organism	Rattus norvegicus(Norway rat)
Product	germ cell-less protein-like 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139642.2, FM064983.1 and BP492225.1. On Apr 28, 2015 this sequence version replaced NM_001010956.2. Transcript Variant: This variant (2) lacks the 3'-terminal exon and has a 3' end that extends into an intron compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY862308.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAGGCA GCTCATTGGT 
TCATCTAACT TATTTGTGAT GCAAGTGGAA ATGGATGTAT ATACAGCTCT GAAAAAGTGG 
ATGTTCCTTC AGTTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAC TCTCTGGTAC CTTCAGAATG GCTGGCTTCA 
GTGTATAAGC AGCAGTGGTT GGCTATGCTT CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AATAGCATGA GGTGTGGTAG AAAGCTTGCC 
AAAGATGGTG AATACTGCTG GCGGTGGACA GGTTTTAATT TTGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TAAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TGCAGCCTCG AAGAAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTCGAC 
AGTAGTGGAA AACTCATATG CAGTAGAGCG ACTGGCTACC AAATCCTGAC GCTTGAGAAA 
GACCAGGTAC GTGTGCTTCT GCGCTTGCGT CTTGTGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010956.1
CDS	211..1692
Translation	MGALSSRVLRPAGRTEQPEPTPGAGGSARRSDAGEDAAHGFCYCPGGRKRKRSSGTFCYCHPESETDEDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDIEALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRTVCGYYTSAGTYGLDSVKKKCLEWLLNNLMTHQSVELFKELSINVMRQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLASVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQVRVLLRLRLVC

Target ORF information:

RefSeq Version	NM_001010956.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010956.3

ORF Insert Sequence:

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAGGCA GCTCATTGGT 
TCATCTAACT TATTTGTGAT GCAAGTGGAA ATGGATGTAT ATACAGCTCT GAAAAAGTGG 
ATGTTCCTTC AGTTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAC TCTCTGGTAC CTTCAGAATG GCTGGCTTCA 
GTGTATAAGC AGCAGTGGTT GGCTATGCTT CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AATAGCATGA GGTGTGGTAG AAAGCTTGCC 
AAAGATGGTG AATACTGCTG GCGGTGGACA GGTTTTAATT TTGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TAAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TGCAGCCTCG AAGAAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTCGAC 
AGTAGTGGAA AACTCATATG CAGTAGAGCG ACTGGCTACC AAATCCTGAC GCTTGAGAAA 
GACCAGGTAC GTGTGCTTCT GCGCTTGCGT CTTGTGTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gmcl1 ( NM_001033931.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029103.1 Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa08750
Clone ID Related Accession (Same CDS sequence)	NM_001033931.2
Accession Version	NM_001033931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-15
Organism	Rattus norvegicus(Norway rat)
Product	germ cell-less protein-like 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139642.2, FM064983.1 and BP492225.1. On Apr 28, 2015 this sequence version replaced NM_001033931.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY862307.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAGGCA GCTCATTGGT 
TCATCTAACT TATTTGTGAT GCAAGTGGAA ATGGATGTAT ATACAGCTCT GAAAAAGTGG 
ATGTTCCTTC AGTTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAC TCTCTGGTAC CTTCAGAATG GCTGGCTTCA 
GTGTATAAGC AGCAGTGGTT GGCTATGCTT CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AATAGCATGA GGTGTGGTAG AAAGCTTGCC 
AAAGATGGTG AATACTGCTG GCGGTGGACA GGTTTTAATT TTGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TAAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TGCAGCCTCG AAGAAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTCGAC 
AGTAGTGGAA AACTCATATG CAGTAGAGCG ACTGGCTACC AAATCCTGAC GCTTGAGAAA 
GACCAGGAGC AAGTGGTGAT GAACTTGGAC AGCAGACTGC TGATCTTCCC TCTATACATC 
TGCTGTAACT TCTTGTATAT ATCACCAGAA AAAAGAACTG AGAGTAATCG TCACCCAGAA 
AACCCAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029103.1
CDS	211..1785
Translation	MGALSSRVLRPAGRTEQPEPTPGAGGSARRSDAGEDAAHGFCYCPGGRKRKRSSGTFCYCHPESETDEDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDIEALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRTVCGYYTSAGTYGLDSVKKKCLEWLLNNLMTHQSVELFKELSINVMRQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLASVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKRTESNRHPENPGH

Target ORF information:

RefSeq Version	NM_001033931.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus germ cell-less 1, spermatogenesis associated (Gmcl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033931.2

ORF Insert Sequence:

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAGGCA GCTCATTGGT 
TCATCTAACT TATTTGTGAT GCAAGTGGAA ATGGATGTAT ATACAGCTCT GAAAAAGTGG 
ATGTTCCTTC AGTTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAC TCTCTGGTAC CTTCAGAATG GCTGGCTTCA 
GTGTATAAGC AGCAGTGGTT GGCTATGCTT CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AATAGCATGA GGTGTGGTAG AAAGCTTGCC 
AAAGATGGTG AATACTGCTG GCGGTGGACA GGTTTTAATT TTGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TAAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TGCAGCCTCG AAGAAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTCGAC 
AGTAGTGGAA AACTCATATG CAGTAGAGCG ACTGGCTACC AAATCCTGAC GCTTGAGAAA 
GACCAGGAGC AAGTGGTGAT GAACTTGGAC AGCAGACTGC TGATCTTCCC TCTATACATC 
TGCTGTAACT TCTTGTATAT ATCACCAGAA AAAAGAACTG AGAGTAATCG TCACCCAGAA 
AACCCAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gmcl1 ( XM_006236832.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236894.1 Rattus norvegicus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27437
Clone ID Related Accession (Same CDS sequence)	XM_006236832.3
Accession Version	XM_006236832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	germ cell-less protein-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAATATA AACGTCATGA GGCAGCTCAT TGGTTCATCT 
AACTTATTTG TGATGCAAGT GGAAATGGAT GTATATACAG CTCTGAAAAA GTGGATGTTC 
CTTCAGTTGG TGCCTTCCTG GAATGGGTCT TTAAAGCAGC TTTTGACAGA AACAGATGTC 
TGGTTTTCAA AGTGGAAAAA AGACTTTGAA GGGACGACTT TCCTTGAAAC TGAGCAGGGA 
AAACCATTTG CGCCCGTGTT CAGACATTTA AGGCTACAGT ACATTATCAG TGATCTGGCT 
TCTGCAAGGA TCATTGAGCA GGACTCTCTG GTACCTTCAG AATGGCTGGC TTCAGTGTAT 
AAGCAGCAGT GGTTGGCTAT GCTTCGGGCT GAACAAGACA GTGAAGTGGG GCCTCAAGAA 
ATCAATAAAG AAGAACTTGA GGGAAATAGC ATGAGGTGTG GTAGAAAGCT TGCCAAAGAT 
GGTGAATACT GCTGGCGGTG GACAGGTTTT AATTTTGGCT TTGACCTCCT TGTGACTTAC 
ACCAATCGAT ACATCATTTT CAAACGCAAT ACGCTAAACC AGCCATGTAG TGGATCTGTC 
AGCTTGCAGC CTCGAAGAAG CATAGCATTT AGATTGCGCT TGGCTTCTTT CGACAGTAGT 
GGAAAACTCA TATGCAGTAG AGCGACTGGC TACCAAATCC TGACGCTTGA GAAAGACCAG 
GAGCAAGTGG TGATGAACTT GGACAGCAGA CTGCTGATCT TCCCTCTATA CATCTGCTGT 
AACTTCTTGT ATATATCACC AGAAAAAAGA ACTGAGAGTA ATCGTCACCC AGAAAACCCA 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236894.1
CDS	234..1742
Translation	MGALSSRVLRPAGRTEQPEPTPGAGGSARRSDAGEDAAHGFCYCPGGRKRKRSSGTFCYCHPESETDEDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDIEALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRTVCGYYTSAGTYGLDSVKKNINVMRQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLASVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKRTESNRHPENPGH

Target ORF information:

RefSeq Version	XM_006236832.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236832.3

ORF Insert Sequence:

ATGGGCGCTC TGAGCAGCCG GGTCCTGCGG CCCGCGGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGTG CTGGGGGCTC GGCCCGCAGA TCGGACGCGG GCGAAGATGC GGCCCACGGC 
TTCTGTTACT GTCCCGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACTTT CTGCTACTGT 
CACCCCGAGT CAGAGACAGA CGAAGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCGAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTTGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTTGTC 
GCCATTTTGG CAGCAGCTTG CATGCTGCAG TTGGATGGTT TGATACAGCA GTGTGGTGAG 
ACCATGAAGG AGACCATCTC TGTAAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAATATA AACGTCATGA GGCAGCTCAT TGGTTCATCT 
AACTTATTTG TGATGCAAGT GGAAATGGAT GTATATACAG CTCTGAAAAA GTGGATGTTC 
CTTCAGTTGG TGCCTTCCTG GAATGGGTCT TTAAAGCAGC TTTTGACAGA AACAGATGTC 
TGGTTTTCAA AGTGGAAAAA AGACTTTGAA GGGACGACTT TCCTTGAAAC TGAGCAGGGA 
AAACCATTTG CGCCCGTGTT CAGACATTTA AGGCTACAGT ACATTATCAG TGATCTGGCT 
TCTGCAAGGA TCATTGAGCA GGACTCTCTG GTACCTTCAG AATGGCTGGC TTCAGTGTAT 
AAGCAGCAGT GGTTGGCTAT GCTTCGGGCT GAACAAGACA GTGAAGTGGG GCCTCAAGAA 
ATCAATAAAG AAGAACTTGA GGGAAATAGC ATGAGGTGTG GTAGAAAGCT TGCCAAAGAT 
GGTGAATACT GCTGGCGGTG GACAGGTTTT AATTTTGGCT TTGACCTCCT TGTGACTTAC 
ACCAATCGAT ACATCATTTT CAAACGCAAT ACGCTAAACC AGCCATGTAG TGGATCTGTC 
AGCTTGCAGC CTCGAAGAAG CATAGCATTT AGATTGCGCT TGGCTTCTTT CGACAGTAGT 
GGAAAACTCA TATGCAGTAG AGCGACTGGC TACCAAATCC TGACGCTTGA GAAAGACCAG 
GAGCAAGTGG TGATGAACTT GGACAGCAGA CTGCTGATCT TCCCTCTATA CATCTGCTGT 
AACTTCTTGT ATATATCACC AGAAAAAAGA ACTGAGAGTA ATCGTCACCC AGAAAACCCA 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and multiple deformations during mgcl-1 deficient spermatogenesis leading to reduced fertility.
Archives of histology and cytology67(4)335-47(2004 Nov)
Maekawa M,Ito C,Toyama Y,Suzuki-Toyota F,Kimura T,Nakano T,Toshimori K

Reduced human germ cell-less (HGCL) expression in azoospermic men with severe germinal cell impairment.
Journal of andrology24(5)670-5(2003 Sep-Oct)
Kleiman SE,Yogev L,Gal-Yam EN,Hauser R,Gamzu R,Botchan A,Paz G,Yavetz H,Maymon BB,Schreiber L,Barzilai S,Amariglio N,Rechavi G,Simon AJ

Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less.
Biochemical and biophysical research communications308(4)927-32(2003 Sep)
Masuhara M,Nagao K,Nishikawa M,Kimura T,Nakano T

Mouse germ cell-less as an essential component for nuclear integrity.
Molecular and cellular biology23(4)1304-15(2003 Feb)
Kimura T,Ito C,Watanabe S,Takahashi T,Ikawa M,Yomogida K,Fujita Y,Ikeuchi M,Asada N,Matsumiya K,Okuyama A,Okabe M,Toshimori K,Nakano T

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Gmcl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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