gmcl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gmcl1
Entrez Gene ID	322146
Full Name	germ cell-less, spermatogenesis associated 1
Synonyms	gcl,wu:fb51d08,zgc:77018
General protein information	Preferred Names germ cell-less, spermatogenesis associated 1 Names germ cell-less protein-like 1 germ cell-less homolog 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207050.1	NP_996933.1	germ cell-less protein-like 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gcl	NP_724708.1
Caenorhabditis elegans (roundworm)	gcl-1	NP_502585.1
Bos taurus (cattle)	GMCL1	NP_001095453.1
Mus musculus (house mouse)	Gmcl1	NP_035948.3
Gallus gallus (chicken)	GMCL1	XP_423987.4
	GMCL1	XP_002799349.1
Rattus norvegicus (Norway rat)	Gmcl1	NP_001029103.1
Homo sapiens (human)	GMCL1	NP_848526.1
Pan troglodytes (chimpanzee)	GMCL1	XP_001148182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011686	XP_320823.3
Canis lupus familiaris (dog)	GMCL1	XP_538534.2
Danio rerio (zebrafish)	gmcl1	NP_996933.1
Xenopus tropicalis (tropical clawed frog)	gmcl1	NP_001072725.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Aanes H, Winata CL, Lin CH, Chen JP, Srinivasan KG, Lee SG, Lim AY, Hajan HS, Collas P, Bourque G, Gong Z, Korzh V, Alestr?m P, Mathavan S
Genome research21(8)1328-38(2011 Aug)

Germ cell-less expression in zebrafish embryos.
Li W, Deng F, Wang H, Zhen Y, Xiang F, Sui Y, Li J
Development, growth & differentiation48(5)333-8(2006 Jun)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that zebrafish germ cell-less (gcl) have potentially important roles in the formation of primordial germ cells.
Title: Germ cell-less expression in zebrafish embryos.

The following gmcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gmcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03745	NM_207050.1 Latest version!	Danio rerio germ cell-less, spermatogenesis associated (gmcl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gmcl1 ( NM_207050.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996933.1 Danio rerio germ cell-less, spermatogenesis associated (gmcl1), mRNA.

Price & Availability↑

CloneID	ODa03745
Clone ID Related Accession (Same CDS sequence)	NM_207050.1
Accession Version	NM_207050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	germ cell-less protein-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066504.1. ##Evidence-Data-START## Transcript exon combination :: BC066504.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGTTCAC TCGGGAGCCG CTTTCAGTCG TCCCCCCGGG GCCCAGAGGA GACTGTGGAG 
CGACAGTGTG CTGGAAACAG ACACAGCTGT GAGTGCAGGA AGAGGAAACG CAGCTTGCGC 
TGTGAGTGTG ACAGCGAGCC GGAAGAAGAG GACAGCCAGC TGGACACGCC ACGCAGGAAG 
AAGTTAAAAA GTACCTCTAA GTACATCTAC CAGACACTGT TCCTGAATGG AGAGAACAGT 
GACATCCAGA TCCGTGCTTT GGGGCAGGAG TGGAACCTTC ATAAAATTTA CCTCTGTCAG 
TCAGGCTATT TCTCCAGCAT GTTCAGTGGA TCCTGGAAGG AGTCTAACAT GATGGTTATT 
GAGCTTGAGA TCCCAGACCA GAACATTGAC ACTGAGGCTC TTCAGGTGGT TTTTGGGTCG 
CTCTACAGGG ATGATGTTCT GATCAAACCG AGTCGAGTTG TTAATATTCT GGCCGCTGCT 
TGCATGCTTC AGTTGGATGG CCTGATCCAG CAGTGTGGAG AGACCATGAA GGAGAATGTA 
AATGTGAAGA CCGTGTGCAG CTATTATAAC TCGGCCACCA TGTACGGCTT GGACTCCGTC 
ATGAAGAAAT GTTTAGAGTG GCTCCTCAAT AACCTGATGA CGCATCAGAA TGTGGAGCTA 
ATGAAGGAGC TCGGGGGAGA CATTATGGAG CAACTCATTC AGTCCTCTGA TCTGTTTGTA 
ATGCAAGTAG AGATGGATGT TTACACGGCA CTGAAGAAGT GGATGTTCTT GCAGCTCAAT 
CCATCTTGGG ACGGTCCAAT AAAGCAACTT CTGCTGGATG CAGATGCCTG GCTGTGTAAA 
AGAAGAAGTG ATGCTGGAGA AGTGGAGCCA TTTCTAAACA CAGATGATGG AATGCCCTTC 
GTCCCTGTGT TCAGACACAT CCGCCTGCAG TACATCATTA ATGACCTGGC CTCAGCCCGC 
ATGCTGGAAC GGGACAACAT CCTACCTCCC GAGTGGCTGA GCTCTGTATA CAAGCAGCAG 
TGGTTCGCCA TGCTGAGGAC TGAACATGAT AATGATAACG GACCTCAGGA GGCCAATAAA 
GAGGAGTTTG AGCTCAACAG TATGCGCTGT GGACGTAAAC TGACCAAAGA TGGAGATTAT 
TGTTGGCGAT GGACGGGGTT TAATTATGGT TTTGACCTGC TGGTGACTTA CACAAACCGC 
TTTATAATCT TCAAGAGGAA CACGCTGAGC CAGCCATGTG GAGGAGCTGT TAGCTTACAG 
CCACGACGAC ATCTAGCGTA CCGATTGCGC CTTGCTTCAT TCGACAGCAG TGGAAAAGTG 
GTTTGCAGTC GTTCAACAGG CTACCAGCTT GTCACCCTCG AAAAAGATCA GGAATATGTG 
GTGATGAATC TGGACAGTCG ACTCTTGTCT TTTCCTCTAT ACGTGTGCTG TAACTTCCTG 
TACACTTCAC CATCCAGCGA GAGGAGAGGA GAAACAGCCG AACCAGAAAG CAGCGCCCGC 
AGTGTGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996933.1
CDS	196..1707
Translation	MGSLGSRFQSSPRGPEETVERQCAGNRHSCECRKRKRSLRCECDSEPEEEDSQLDTPRRKKLKSTSKYIYQTLFLNGENSDIQIRALGQEWNLHKIYLCQSGYFSSMFSGSWKESNMMVIELEIPDQNIDTEALQVVFGSLYRDDVLIKPSRVVNILAAACMLQLDGLIQQCGETMKENVNVKTVCSYYNSATMYGLDSVMKKCLEWLLNNLMTHQNVELMKELGGDIMEQLIQSSDLFVMQVEMDVYTALKKWMFLQLNPSWDGPIKQLLLDADAWLCKRRSDAGEVEPFLNTDDGMPFVPVFRHIRLQYIINDLASARMLERDNILPPEWLSSVYKQQWFAMLRTEHDNDNGPQEANKEEFELNSMRCGRKLTKDGDYCWRWTGFNYGFDLLVTYTNRFIIFKRNTLSQPCGGAVSLQPRRHLAYRLRLASFDSSGKVVCSRSTGYQLVTLEKDQEYVVMNLDSRLLSFPLYVCCNFLYTSPSSERRGETAEPESSARSVS

Target ORF information:

RefSeq Version	NM_207050.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio germ cell-less, spermatogenesis associated (gmcl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207050.1

ORF Insert Sequence:

ATGGGTTCAC TCGGGAGCCG CTTTCAGTCG TCCCCCCGGG GCCCAGAGGA GACTGTGGAG 
CGACAGTGTG CTGGAAACAG ACACAGCTGT GAGTGCAGGA AGAGGAAACG CAGCTTGCGC 
TGTGAGTGTG ACAGCGAGCC GGAAGAAGAG GACAGCCAGC TGGACACGCC ACGCAGGAAG 
AAGTTAAAAA GTACCTCTAA GTACATCTAC CAGACACTGT TCCTGAATGG AGAGAACAGT 
GACATCCAGA TCCGTGCTTT GGGGCAGGAG TGGAACCTTC ATAAAATTTA CCTCTGTCAG 
TCAGGCTATT TCTCCAGCAT GTTCAGTGGA TCCTGGAAGG AGTCTAACAT GATGGTTATT 
GAGCTTGAGA TCCCAGACCA GAACATTGAC ACTGAGGCTC TTCAGGTGGT TTTTGGGTCG 
CTCTACAGGG ATGATGTTCT GATCAAACCG AGTCGAGTTG TTAATATTCT GGCCGCTGCT 
TGCATGCTTC AGTTGGATGG CCTGATCCAG CAGTGTGGAG AGACCATGAA GGAGAATGTA 
AATGTGAAGA CCGTGTGCAG CTATTATAAC TCGGCCACCA TGTACGGCTT GGACTCCGTC 
ATGAAGAAAT GTTTAGAGTG GCTCCTCAAT AACCTGATGA CGCATCAGAA TGTGGAGCTA 
ATGAAGGAGC TCGGGGGAGA CATTATGGAG CAACTCATTC AGTCCTCTGA TCTGTTTGTA 
ATGCAAGTAG AGATGGATGT TTACACGGCA CTGAAGAAGT GGATGTTCTT GCAGCTCAAT 
CCATCTTGGG ACGGTCCAAT AAAGCAACTT CTGCTGGATG CAGATGCCTG GCTGTGTAAA 
AGAAGAAGTG ATGCTGGAGA AGTGGAGCCA TTTCTAAACA CAGATGATGG AATGCCCTTC 
GTCCCTGTGT TCAGACACAT CCGCCTGCAG TACATCATTA ATGACCTGGC CTCAGCCCGC 
ATGCTGGAAC GGGACAACAT CCTACCTCCC GAGTGGCTGA GCTCTGTATA CAAGCAGCAG 
TGGTTCGCCA TGCTGAGGAC TGAACATGAT AATGATAACG GACCTCAGGA GGCCAATAAA 
GAGGAGTTTG AGCTCAACAG TATGCGCTGT GGACGTAAAC TGACCAAAGA TGGAGATTAT 
TGTTGGCGAT GGACGGGGTT TAATTATGGT TTTGACCTGC TGGTGACTTA CACAAACCGC 
TTTATAATCT TCAAGAGGAA CACGCTGAGC CAGCCATGTG GAGGAGCTGT TAGCTTACAG 
CCACGACGAC ATCTAGCGTA CCGATTGCGC CTTGCTTCAT TCGACAGCAG TGGAAAAGTG 
GTTTGCAGTC GTTCAACAGG CTACCAGCTT GTCACCCTCG AAAAAGATCA GGAATATGTG 
GTGATGAATC TGGACAGTCG ACTCTTGTCT TTTCCTCTAT ACGTGTGCTG TAACTTCCTG 
TACACTTCAC CATCCAGCGA GAGGAGAGGA GAAACAGCCG AACCAGAAAG CAGCGCCCGC 
AGTGTGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Genome research21(8)1328-38(2011 Aug)
Aanes H,Winata CL,Lin CH,Chen JP,Srinivasan KG,Lee SG,Lim AY,Hajan HS,Collas P,Bourque G,Gong Z,Korzh V,Alestr?m P,Mathavan S

Germ cell-less expression in zebrafish embryos.
Development, growth & differentiation48(5)333-8(2006 Jun)
Li W,Deng F,Wang H,Zhen Y,Xiang F,Sui Y,Li J

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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gmcl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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