Gmcl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gmcl1
Entrez Gene ID	23885
Full Name	germ cell-less, spermatogenesis associated 1
Synonyms	2810049L19Rik,Btbd13,DIP,Gcl,Gcl-1,glc-1,mglc-1
General protein information	Preferred Names germ cell-less, spermatogenesis associated 1 Names germ cell-less protein-like 1 DP-interacting protein germ cell-less homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 37.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506056.3	XP_006506119.1	germ cell-less protein-like 1 isoform X1
XM_006506056.2	XP_006506119.1	germ cell-less protein-like 1 isoform X1
NM_011818.3	NP_035948.3	germ cell-less protein-like 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gcl	NP_724708.1
Caenorhabditis elegans (roundworm)	gcl-1	NP_502585.1
Bos taurus (cattle)	GMCL1	NP_001095453.1
Mus musculus (house mouse)	Gmcl1	NP_035948.3
Gallus gallus (chicken)	GMCL1	XP_423987.4
	GMCL1	XP_002799349.1
Rattus norvegicus (Norway rat)	Gmcl1	NP_001029103.1
Homo sapiens (human)	GMCL1	NP_848526.1
Pan troglodytes (chimpanzee)	GMCL1	XP_001148182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011686	XP_320823.3
Canis lupus familiaris (dog)	GMCL1	XP_538534.2
Danio rerio (zebrafish)	gmcl1	NP_996933.1
Xenopus tropicalis (tropical clawed frog)	gmcl1	NP_001072725.1

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages.
Liebe B, Petukhova G, Barchi M, Bellani M, Braselmann H, Nakano T, Pandita TK, Jasin M, Fornace A, Meistrich ML, Baarends WM, Schimenti J, de Lange T, Keeney S, Camerini-Otero RD, Scherthan H
Experimental cell research312(19)3768-81(2006 Nov)

Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and multiple deformations during mgcl-1 deficient spermatogenesis leading to reduced fertility.
Maekawa M, Ito C, Toyama Y, Suzuki-Toyota F, Kimura T, Nakano T, Toshimori K
Archives of histology and cytology67(4)335-47(2004 Nov)

Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less.
Masuhara M, Nagao K, Nishikawa M, Kimura T, Nakano T
Biochemical and biophysical research communications308(4)927-32(2003 Sep)

Mouse germ cell-less as an essential component for nuclear integrity.
Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, Toshimori K, Nakano T
Molecular and cellular biology23(4)1304-15(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mGCL-1 is a factor modulating MDM2-p53 axis by enhanced degradation of MDM2
Title: Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less.

the mgcl-1 gene product appears to be essential for appropriate nuclear-lamina organization, which in turn is essential for normal sperm morphogenesis and chromatin remodeling.
Title: Mouse germ cell-less as an essential component for nuclear integrity.

Gmcl1 interacts with the DP component of the E2F transcription factor.
Title: Integration of a growth-suppressing BTB/POZ domain protein with the DP component of the E2F transcription factor.

The following Gmcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gmcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu37230	XM_006506056.3 Latest version!	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu08915	NM_011818.3 Latest version!	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu37230	XM_006506056.2	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gmcl1 ( XM_006506056.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506119.1 Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37230
Clone ID Related Accession (Same CDS sequence)	XM_006506056.3 , XM_006506056.2
Accession Version	XM_006506056.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	germ cell-less protein-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACC AGAGTGTGGA GCTTTTCAAA GAACTCAGTA TAAACGTCAT GAAACAGCTC 
ATTGGTTCGT CTAACTTATT TGTGATGCAA GTGGAGATGG ATGTATATAC AGCTCTTAAA 
AAGTGGATGT TCCTTCAGCT GGTGCCTTCC TGGAATGGGT CTTTAAAGCA GCTTTTGACA 
GAAACAGATG TCTGGTTTTC AAAGTGGAAA AAAGACTTTG AAGGGACGAC TTTCCTTGAA 
ACTGAGCAGG GAAAACCATT TGCGCCCGTG TTCAGACATT TAAGGCTACA GTACATTATC 
AGTGATCTGG CTTCTGCAAG GATCATTGAG CAGGATTCTC TGGTACCTTC AGAATGGCTG 
GCGGCAGTGT ATAAACAGCA GTGGCTGGCT ATGCTACGGG CTGAACAAGA CAGTGAAGTG 
GGGCCTCAAG AAATCAATAA AGAAGAACTT GAGGGAAACA GCATGAGGTG TGGTCGAAAG 
CTTGCCAAAG ATGGTGAGTA CTGCTGGCGC TGGACAGGCT TCAATTTCGG CTTTGACCTC 
CTTGTGACTT ACACCAATCG ATACATCATT TTCAAACGCA ATACGCTGAA CCAGCCATGT 
AGTGGATCTG TCAGCTTACA GCCTCGAAGG AGCATAGCAT TTAGATTGCG CTTGGCTTCT 
TTTGACAGTA GTGGGAAACT CATATGCAGT AGAGCAACTG GCTACCAAAT ACTGACGCTT 
GAAAAAGACC AGGAGCAAGT GGTGATGAAC TTGGACAGCA GACTTCTGAT CTTCCCTCTG 
TACATCTGCT GTAACTTCTT GTATATATCA CCAGAAAAAA GAACTGAGAG TAATCGTCAC 
CCAGAAAACC CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506119.1
CDS	523..1383
Translation	MTHQSVELFKELSINVMKQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLAAVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKRTESNRHPENPGH

Target ORF information:

RefSeq Version	XM_006506056.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506056.2

ORF Insert Sequence:

ATGACTCACC AGAGTGTGGA GCTTTTCAAA GAACTCAGTA TAAACGTCAT GAAACAGCTC 
ATTGGTTCGT CTAACTTATT TGTGATGCAA GTGGAGATGG ATGTATATAC AGCTCTTAAA 
AAGTGGATGT TCCTTCAGCT GGTGCCTTCC TGGAATGGGT CTTTAAAGCA GCTTTTGACA 
GAAACAGATG TCTGGTTTTC AAAGTGGAAA AAAGACTTTG AAGGGACGAC TTTCCTTGAA 
ACTGAGCAGG GAAAACCATT TGCGCCCGTG TTCAGACATT TAAGGCTACA GTACATTATC 
AGTGATCTGG CTTCTGCAAG GATCATTGAG CAGGATTCTC TGGTACCTTC AGAATGGCTG 
GCGGCAGTGT ATAAACAGCA GTGGCTGGCT ATGCTACGGG CTGAACAAGA CAGTGAAGTG 
GGGCCTCAAG AAATCAATAA AGAAGAACTT GAGGGAAACA GCATGAGGTG TGGTCGAAAG 
CTTGCCAAAG ATGGTGAGTA CTGCTGGCGC TGGACAGGCT TCAATTTCGG CTTTGACCTC 
CTTGTGACTT ACACCAATCG ATACATCATT TTCAAACGCA ATACGCTGAA CCAGCCATGT 
AGTGGATCTG TCAGCTTACA GCCTCGAAGG AGCATAGCAT TTAGATTGCG CTTGGCTTCT 
TTTGACAGTA GTGGGAAACT CATATGCAGT AGAGCAACTG GCTACCAAAT ACTGACGCTT 
GAAAAAGACC AGGAGCAAGT GGTGATGAAC TTGGACAGCA GACTTCTGAT CTTCCCTCTG 
TACATCTGCT GTAACTTCTT GTATATATCA CCAGAAAAAA GAACTGAGAG TAATCGTCAC 
CCAGAAAACC CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gmcl1 ( XM_006506056.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506119.1 Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu37230
Clone ID Related Accession (Same CDS sequence)	XM_006506056.3 , XM_006506056.2
Accession Version	XM_006506056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	germ cell-less protein-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACC AGAGTGTGGA GCTTTTCAAA GAACTCAGTA TAAACGTCAT GAAACAGCTC 
ATTGGTTCGT CTAACTTATT TGTGATGCAA GTGGAGATGG ATGTATATAC AGCTCTTAAA 
AAGTGGATGT TCCTTCAGCT GGTGCCTTCC TGGAATGGGT CTTTAAAGCA GCTTTTGACA 
GAAACAGATG TCTGGTTTTC AAAGTGGAAA AAAGACTTTG AAGGGACGAC TTTCCTTGAA 
ACTGAGCAGG GAAAACCATT TGCGCCCGTG TTCAGACATT TAAGGCTACA GTACATTATC 
AGTGATCTGG CTTCTGCAAG GATCATTGAG CAGGATTCTC TGGTACCTTC AGAATGGCTG 
GCGGCAGTGT ATAAACAGCA GTGGCTGGCT ATGCTACGGG CTGAACAAGA CAGTGAAGTG 
GGGCCTCAAG AAATCAATAA AGAAGAACTT GAGGGAAACA GCATGAGGTG TGGTCGAAAG 
CTTGCCAAAG ATGGTGAGTA CTGCTGGCGC TGGACAGGCT TCAATTTCGG CTTTGACCTC 
CTTGTGACTT ACACCAATCG ATACATCATT TTCAAACGCA ATACGCTGAA CCAGCCATGT 
AGTGGATCTG TCAGCTTACA GCCTCGAAGG AGCATAGCAT TTAGATTGCG CTTGGCTTCT 
TTTGACAGTA GTGGGAAACT CATATGCAGT AGAGCAACTG GCTACCAAAT ACTGACGCTT 
GAAAAAGACC AGGAGCAAGT GGTGATGAAC TTGGACAGCA GACTTCTGAT CTTCCCTCTG 
TACATCTGCT GTAACTTCTT GTATATATCA CCAGAAAAAA GAACTGAGAG TAATCGTCAC 
CCAGAAAACC CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506119.1
CDS	1004..1864
Translation	MTHQSVELFKELSINVMKQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLAAVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKRTESNRHPENPGH

Target ORF information:

RefSeq Version	XM_006506056.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506056.3

ORF Insert Sequence:

ATGACTCACC AGAGTGTGGA GCTTTTCAAA GAACTCAGTA TAAACGTCAT GAAACAGCTC 
ATTGGTTCGT CTAACTTATT TGTGATGCAA GTGGAGATGG ATGTATATAC AGCTCTTAAA 
AAGTGGATGT TCCTTCAGCT GGTGCCTTCC TGGAATGGGT CTTTAAAGCA GCTTTTGACA 
GAAACAGATG TCTGGTTTTC AAAGTGGAAA AAAGACTTTG AAGGGACGAC TTTCCTTGAA 
ACTGAGCAGG GAAAACCATT TGCGCCCGTG TTCAGACATT TAAGGCTACA GTACATTATC 
AGTGATCTGG CTTCTGCAAG GATCATTGAG CAGGATTCTC TGGTACCTTC AGAATGGCTG 
GCGGCAGTGT ATAAACAGCA GTGGCTGGCT ATGCTACGGG CTGAACAAGA CAGTGAAGTG 
GGGCCTCAAG AAATCAATAA AGAAGAACTT GAGGGAAACA GCATGAGGTG TGGTCGAAAG 
CTTGCCAAAG ATGGTGAGTA CTGCTGGCGC TGGACAGGCT TCAATTTCGG CTTTGACCTC 
CTTGTGACTT ACACCAATCG ATACATCATT TTCAAACGCA ATACGCTGAA CCAGCCATGT 
AGTGGATCTG TCAGCTTACA GCCTCGAAGG AGCATAGCAT TTAGATTGCG CTTGGCTTCT 
TTTGACAGTA GTGGGAAACT CATATGCAGT AGAGCAACTG GCTACCAAAT ACTGACGCTT 
GAAAAAGACC AGGAGCAAGT GGTGATGAAC TTGGACAGCA GACTTCTGAT CTTCCCTCTG 
TACATCTGCT GTAACTTCTT GTATATATCA CCAGAAAAAA GAACTGAGAG TAATCGTCAC 
CCAGAAAACC CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gmcl1 ( NM_011818.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035948.3 Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), mRNA.

Price & Availability↑

CloneID	OMu08915
Clone ID Related Accession (Same CDS sequence)	NM_011818.3
Accession Version	NM_011818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	germ cell-less protein-like 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ122611.1, AF163665.1, AK004716.1 and CX567687.1. On Nov 15, 2007 this sequence version replaced NM_011818.2. ##Evidence-Data-START## Transcript exon combination :: AF186095.1, AK155238.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCTC TCAGCAGCCG GGTGCTGCGG CCCGCAGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGGG CTGGGGGCGC GGCCCGCAGG TCGGACGCCG GCGAAGATGC GGGCCACAGC 
TTCTGTTACT GTCCGGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACATT CTGCTACTGT 
CACCCCGACT CCGAGACAGA CGACGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCAAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTCGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTCGTT 
GCCATTTTGG CAGCAGCTTG CATGCTGCAA TTGGATGGTT TGATACAGCA GTGCGGTGAG 
ACAATGAAGG AGACCATCTC TGTGAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAAACA GCTCATTGGT 
TCGTCTAACT TATTTGTGAT GCAAGTGGAG ATGGATGTAT ATACAGCTCT TAAAAAGTGG 
ATGTTCCTTC AGCTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAT TCTCTGGTAC CTTCAGAATG GCTGGCGGCA 
GTGTATAAAC AGCAGTGGCT GGCTATGCTA CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AACAGCATGA GGTGTGGTCG AAAGCTTGCC 
AAAGATGGTG AGTACTGCTG GCGCTGGACA GGCTTCAATT TCGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TGAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TACAGCCTCG AAGGAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTTGAC 
AGTAGTGGGA AACTCATATG CAGTAGAGCA ACTGGCTACC AAATACTGAC GCTTGAAAAA 
GACCAGGAGC AAGTGGTGAT GAACTTGGAC AGCAGACTTC TGATCTTCCC TCTGTACATC 
TGCTGTAACT TCTTGTATAT ATCACCAGAA AAAAGAACTG AGAGTAATCG TCACCCAGAA 
AACCCAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035948.3
CDS	215..1789
Translation	MGALSSRVLRPAGRTEQPEPTPGAGGAARRSDAGEDAGHSFCYCPGGRKRKRSSGTFCYCHPDSETDDDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDIEALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRTVCGYYTSAGTYGLDSVKKKCLEWLLNNLMTHQSVELFKELSINVMKQLIGSSNLFVMQVEMDVYTALKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQDSLVPSEWLAAVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKRTESNRHPENPGH

Target ORF information:

RefSeq Version	NM_011818.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus germ cell-less, spermatogenesis associated 1 (Gmcl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011818.3

ORF Insert Sequence:

ATGGGCGCTC TCAGCAGCCG GGTGCTGCGG CCCGCAGGGC GCACAGAGCA GCCCGAACCC 
ACGCCCGGGG CTGGGGGCGC GGCCCGCAGG TCGGACGCCG GCGAAGATGC GGGCCACAGC 
TTCTGTTACT GTCCGGGCGG CCGCAAGCGC AAGCGCAGCA GCGGCACATT CTGCTACTGT 
CACCCCGACT CCGAGACAGA CGACGACGAG GACGAGGGCG ACGAGCAGCA GAGGCTGCTG 
AACACGCCGC GCAGGAAAAA ATTAAAGAGC ACATCAAAAT ACATCTACCA AACGCTGTTT 
TTGAATGGTG AAAACAGTGA CATTAAGATC TGTGCTCTAG GTGAAGAGTG GAGCTTACAC 
AAAATCTACT TATGTCAATC TGGCTACTTT TCTAGTATGT TCAGTGGTTC TTGGAAAGAA 
TCCAGCATGA ATATTATTGA ACTGGAGATT CCTGACCAGA ACATTGATAT AGAAGCACTG 
CAGGTCGCAT TTGGATCACT GTATCGAGAT GACGTCTTAA TAAAGCCCAG CAGGGTCGTT 
GCCATTTTGG CAGCAGCTTG CATGCTGCAA TTGGATGGTT TGATACAGCA GTGCGGTGAG 
ACAATGAAGG AGACCATCTC TGTGAGAACT GTGTGTGGCT ATTACACATC GGCAGGGACC 
TATGGACTAG ACTCTGTAAA GAAAAAGTGC CTCGAGTGGC TGCTGAACAA CCTCATGACT 
CACCAGAGTG TGGAGCTTTT CAAAGAACTC AGTATAAACG TCATGAAACA GCTCATTGGT 
TCGTCTAACT TATTTGTGAT GCAAGTGGAG ATGGATGTAT ATACAGCTCT TAAAAAGTGG 
ATGTTCCTTC AGCTGGTGCC TTCCTGGAAT GGGTCTTTAA AGCAGCTTTT GACAGAAACA 
GATGTCTGGT TTTCAAAGTG GAAAAAAGAC TTTGAAGGGA CGACTTTCCT TGAAACTGAG 
CAGGGAAAAC CATTTGCGCC CGTGTTCAGA CATTTAAGGC TACAGTACAT TATCAGTGAT 
CTGGCTTCTG CAAGGATCAT TGAGCAGGAT TCTCTGGTAC CTTCAGAATG GCTGGCGGCA 
GTGTATAAAC AGCAGTGGCT GGCTATGCTA CGGGCTGAAC AAGACAGTGA AGTGGGGCCT 
CAAGAAATCA ATAAAGAAGA ACTTGAGGGA AACAGCATGA GGTGTGGTCG AAAGCTTGCC 
AAAGATGGTG AGTACTGCTG GCGCTGGACA GGCTTCAATT TCGGCTTTGA CCTCCTTGTG 
ACTTACACCA ATCGATACAT CATTTTCAAA CGCAATACGC TGAACCAGCC ATGTAGTGGA 
TCTGTCAGCT TACAGCCTCG AAGGAGCATA GCATTTAGAT TGCGCTTGGC TTCTTTTGAC 
AGTAGTGGGA AACTCATATG CAGTAGAGCA ACTGGCTACC AAATACTGAC GCTTGAAAAA 
GACCAGGAGC AAGTGGTGAT GAACTTGGAC AGCAGACTTC TGATCTTCCC TCTGTACATC 
TGCTGTAACT TCTTGTATAT ATCACCAGAA AAAAGAACTG AGAGTAATCG TCACCCAGAA 
AACCCAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages.
Experimental cell research312(19)3768-81(2006 Nov)
Liebe B,Petukhova G,Barchi M,Bellani M,Braselmann H,Nakano T,Pandita TK,Jasin M,Fornace A,Meistrich ML,Baarends WM,Schimenti J,de Lange T,Keeney S,Camerini-Otero RD,Scherthan H

Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and multiple deformations during mgcl-1 deficient spermatogenesis leading to reduced fertility.
Archives of histology and cytology67(4)335-47(2004 Nov)
Maekawa M,Ito C,Toyama Y,Suzuki-Toyota F,Kimura T,Nakano T,Toshimori K

Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less.
Biochemical and biophysical research communications308(4)927-32(2003 Sep)
Masuhara M,Nagao K,Nishikawa M,Kimura T,Nakano T

Mouse germ cell-less as an essential component for nuclear integrity.
Molecular and cellular biology23(4)1304-15(2003 Feb)
Kimura T,Ito C,Watanabe S,Takahashi T,Ikawa M,Yomogida K,Fujita Y,Ikeuchi M,Asada N,Matsumiya K,Okuyama A,Okabe M,Toshimori K,Nakano T

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Gmcl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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