gcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gcl
Entrez Gene ID	35864
Full Name	germ cell-less
Synonyms	CG8411,Dmel\CG8411,GCL,Gcl,gcl-1
General protein information	Preferred Names germ cell-less Names CG8411 gene product from transcript CG8411-RB CG8411-PA CG8411-PB gcl-PA gcl-PB germ cell less germ-cell-less
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44E3-44E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165622.2	NP_724708.1	germ cell-less, isoform A
NM_001299281.1	NP_001286210.1	germ cell-less, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gcl	NP_724708.1
Caenorhabditis elegans (roundworm)	gcl-1	NP_502585.1
Bos taurus (cattle)	GMCL1	NP_001095453.1
Mus musculus (house mouse)	Gmcl1	NP_035948.3
Gallus gallus (chicken)	GMCL1	XP_423987.4
	GMCL1	XP_002799349.1
Rattus norvegicus (Norway rat)	Gmcl1	NP_001029103.1
Homo sapiens (human)	GMCL1	NP_848526.1
Pan troglodytes (chimpanzee)	GMCL1	XP_001148182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011686	XP_320823.3
Canis lupus familiaris (dog)	GMCL1	XP_538534.2
Danio rerio (zebrafish)	gmcl1	NP_996933.1
Xenopus tropicalis (tropical clawed frog)	gmcl1	NP_001072725.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRL3(GCL) promotes primordial germ cell fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate.
Title: GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.

Repression by Bruno during oogenesis is required to restrict Gcl expression in the early embryo.
Title: Bruno negatively regulates germ cell-less expression in a BRE-independent manner.

Germ cell-less (GCL), a germ plasm component necessary for the proper formation of "pole cells," the germ cell precursors in Drosophila, is required for the establishment of this transcriptional quiescence
Title: germ cell-less acts to repress transcription during the establishment of the Drosophila germ cell lineage.

The following gcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12802	NM_165622.2 Latest version!	Drosophila melanogaster germ cell-less (gcl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21718	NM_001299281.1 Latest version!	Drosophila melanogaster germ cell-less (gcl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcl ( NM_165622.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724708.1 Drosophila melanogaster germ cell-less (gcl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12802
Clone ID Related Accession (Same CDS sequence)	NM_165622.2
Accession Version	NM_165622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	germ cell-less, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165622.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCAAA TAGTGGGATC CATGCATATG AACGTCGCTG AGGTGTTCAG CAATCGACGG 
AAGCGGAAGC GCAGCACTGA TTCATCTTTG GGGAAGGATG ATCCGGCCCA GTTAGACACC 
ACTCAGCCCA AAAAAAAAAA ACTTCTTACT ACCACCCAGT ACATATACAA GGCGCTCTTC 
AAGGAGGAAA AGAACTCGGA TGTGGCTGTC ATGGCTCTGG ATAAGGTCTG GCACCTGCAT 
AAGGTGTACC TGAGCCAGAG TCCATACTTC TATACTATGT TCAATGGGAC GTGGCGGGAA 
GCCCAGCAGA ATTTCATTCA GATCACAATC CTGGACGACC GGATCACCGT TGCTAGCCTG 
GATGCTGTCT TTGGTTCCAT GTATTCCGAT GAGATCGAAA TCGAGTCAGC AGATGTTATC 
TCGGTATTGG CCACAGCCAC GTTGTTCCAC TTAGACGGAA TCATCGACAA GTGTGCCGAG 
GTGATGGTGG ATAACATTAG TCCGGAGACA GCTATCCAGT ATTACGAGGC CGCCTGCCAG 
TACGGTGTGG TCGGAGTCAA GAAGTCCACC TTTCAGTGGT TCCAGATCAA TCTGCTAAGC 
ATTTATAGTA AGCAACCGAA CCTGCTTAGG CACATCTCCA TTGAGCTGAT GAGTGCCCTG 
ACCGCCAGTC CCGATTTGTA TGTGATGCAG ACGGAGTTCT CGCTGTACAC TCTACTGCGC 
ACATGGATGT TCCTACGACT GCATCCCGAC TACGATCCAG AGGACCCGGT CCAGCGGGCG 
GAGGCGCTAA AGACGCAGGA GCTTTTGGTT AATGCCGGCG TGGAAACGCA TGCGCCTAGT 
GGTGATGTCG TCCAGTGGAC ATACTTTACC AGCCGCTCGG AGGAACGCTC GTTCTTGGCC 
ACGCCCGAGG GGCAGCCTTA TGTGAAGGTC TTTCAGAAGC TACGAACCCA GTACCTGACC 
AACCACTACA TGGATCTAAA AATCATATAC AATGACAATA TTATACCCAA GGAGTGGCTG 
TATCGGCACA TTCACAACCA CTGGGATGCA TTACTACGTA TCGATCATGG GCAGGAGGAT 
TGTAGTCCTC AGCAGCTGGA CGATGAGCAG TTCTTTGAGA ACTGCATGCG ATGCGGACGT 
ATGTTGCTTG AGCCTGGCTA CCAGAAGTGG CGCTGGACGG GCTTCAACTT TGGCATGGAT 
CTCATCCTGA TCATGGACTC GCGTAGACTG AACATTCGTC GGCACCACCG GCACGAGCAC 
GAGCGAGTGC TTAGCCTGCA GACGAAACGC AAGTTCATGG TCCGCACCAC GGTGACCTCG 
ATAAACGCCC AGCGCCAGGC AGTCTTCACC CAAACGTCCG AGATCTGTTC TCTTAGCCTC 
GAAAAGAACG AGGAGGTGCC ACTCATGGTG TTAGACCCAA AGTTGGTGCA TCCACTTCTC 
ATTTCAATAA ACATGCTGGT GGTAATGCCA CCGAATCAGA GTTTCAAGGA AATTGTTCCG 
CTCAGCGAGG AGGCGACAAC CTCTCTCTCC ATACCCATTT CGGAAATCGG AGCAAACAGC 
GACAGACCGC TGTCCCCGTC CAGTGCTGAT GATTCGGCCG TTTTCATTGG TGACTCTGAG 
CCTTCGACGC CATCCTCGCC AGCCCCACGG CCCAGAATCG CTTGGTCGGC TAGCGAAACG 
GGCGCAATCT GCGGACAACT GGCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724708.1
CDS	231..1940
Translation	MGQIVGSMHMNVAEVFSNRRKRKRSTDSSLGKDDPAQLDTTQPKKKKLLTTTQYIYKALFKEEKNSDVAVMALDKVWHLHKVYLSQSPYFYTMFNGTWREAQQNFIQITILDDRITVASLDAVFGSMYSDEIEIESADVISVLATATLFHLDGIIDKCAEVMVDNISPETAIQYYEAACQYGVVGVKKSTFQWFQINLLSIYSKQPNLLRHISIELMSALTASPDLYVMQTEFSLYTLLRTWMFLRLHPDYDPEDPVQRAEALKTQELLVNAGVETHAPSGDVVQWTYFTSRSEERSFLATPEGQPYVKVFQKLRTQYLTNHYMDLKIIYNDNIIPKEWLYRHIHNHWDALLRIDHGQEDCSPQQLDDEQFFENCMRCGRMLLEPGYQKWRWTGFNFGMDLILIMDSRRLNIRRHHRHEHERVLSLQTKRKFMVRTTVTSINAQRQAVFTQTSEICSLSLEKNEEVPLMVLDPKLVHPLLISINMLVVMPPNQSFKEIVPLSEEATTSLSIPISEIGANSDRPLSPSSADDSAVFIGDSEPSTPSSPAPRPRIAWSASETGAICGQLAC

Target ORF information:

RefSeq Version	NM_165622.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster germ cell-less (gcl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165622.2

ORF Insert Sequence:

ATGGGTCAAA TAGTGGGATC CATGCATATG AACGTCGCTG AGGTGTTCAG CAATCGACGG 
AAGCGGAAGC GCAGCACTGA TTCATCTTTG GGGAAGGATG ATCCGGCCCA GTTAGACACC 
ACTCAGCCCA AAAAAAAAAA ACTTCTTACT ACCACCCAGT ACATATACAA GGCGCTCTTC 
AAGGAGGAAA AGAACTCGGA TGTGGCTGTC ATGGCTCTGG ATAAGGTCTG GCACCTGCAT 
AAGGTGTACC TGAGCCAGAG TCCATACTTC TATACTATGT TCAATGGGAC GTGGCGGGAA 
GCCCAGCAGA ATTTCATTCA GATCACAATC CTGGACGACC GGATCACCGT TGCTAGCCTG 
GATGCTGTCT TTGGTTCCAT GTATTCCGAT GAGATCGAAA TCGAGTCAGC AGATGTTATC 
TCGGTATTGG CCACAGCCAC GTTGTTCCAC TTAGACGGAA TCATCGACAA GTGTGCCGAG 
GTGATGGTGG ATAACATTAG TCCGGAGACA GCTATCCAGT ATTACGAGGC CGCCTGCCAG 
TACGGTGTGG TCGGAGTCAA GAAGTCCACC TTTCAGTGGT TCCAGATCAA TCTGCTAAGC 
ATTTATAGTA AGCAACCGAA CCTGCTTAGG CACATCTCCA TTGAGCTGAT GAGTGCCCTG 
ACCGCCAGTC CCGATTTGTA TGTGATGCAG ACGGAGTTCT CGCTGTACAC TCTACTGCGC 
ACATGGATGT TCCTACGACT GCATCCCGAC TACGATCCAG AGGACCCGGT CCAGCGGGCG 
GAGGCGCTAA AGACGCAGGA GCTTTTGGTT AATGCCGGCG TGGAAACGCA TGCGCCTAGT 
GGTGATGTCG TCCAGTGGAC ATACTTTACC AGCCGCTCGG AGGAACGCTC GTTCTTGGCC 
ACGCCCGAGG GGCAGCCTTA TGTGAAGGTC TTTCAGAAGC TACGAACCCA GTACCTGACC 
AACCACTACA TGGATCTAAA AATCATATAC AATGACAATA TTATACCCAA GGAGTGGCTG 
TATCGGCACA TTCACAACCA CTGGGATGCA TTACTACGTA TCGATCATGG GCAGGAGGAT 
TGTAGTCCTC AGCAGCTGGA CGATGAGCAG TTCTTTGAGA ACTGCATGCG ATGCGGACGT 
ATGTTGCTTG AGCCTGGCTA CCAGAAGTGG CGCTGGACGG GCTTCAACTT TGGCATGGAT 
CTCATCCTGA TCATGGACTC GCGTAGACTG AACATTCGTC GGCACCACCG GCACGAGCAC 
GAGCGAGTGC TTAGCCTGCA GACGAAACGC AAGTTCATGG TCCGCACCAC GGTGACCTCG 
ATAAACGCCC AGCGCCAGGC AGTCTTCACC CAAACGTCCG AGATCTGTTC TCTTAGCCTC 
GAAAAGAACG AGGAGGTGCC ACTCATGGTG TTAGACCCAA AGTTGGTGCA TCCACTTCTC 
ATTTCAATAA ACATGCTGGT GGTAATGCCA CCGAATCAGA GTTTCAAGGA AATTGTTCCG 
CTCAGCGAGG AGGCGACAAC CTCTCTCTCC ATACCCATTT CGGAAATCGG AGCAAACAGC 
GACAGACCGC TGTCCCCGTC CAGTGCTGAT GATTCGGCCG TTTTCATTGG TGACTCTGAG 
CCTTCGACGC CATCCTCGCC AGCCCCACGG CCCAGAATCG CTTGGTCGGC TAGCGAAACG 
GGCGCAATCT GCGGACAACT GGCGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcl ( NM_001299281.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286210.1 Drosophila melanogaster germ cell-less (gcl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21718
Clone ID Related Accession (Same CDS sequence)	NM_001299281.1
Accession Version	NM_001299281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	germ cell-less, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCAAA TAGTGGGATC CATGCATATG AACGTCGCTG AGGTGTTCAG CAATCGACGG 
AAGCGGAAGC GCAGCACTGA TTCATCTTTG GGGAAGGATG ATCCGGCCCA GTTAGACACC 
ACTCAGCCCA AAAAAAAAAA ACTTCTTACT ACCACCCAGT ACATATACAA GGCGCTCTTC 
AAGGAGGAAA AGAACTCGGA TGTGGCTGTC ATGGCTCTGG ATAAGGTCTG GCACCTGCAT 
AAGGTGTACC TGAGCCAGAG TCCATACTTC TATACTATGT TCAATGGGAC GTGGCGGGAA 
GCCCAGCAGA ATTTCATTCA GATCACAATC CTGGACGACC GGATCACCGT TGCTAGCCTG 
GATGCTGTCT TTGGTTCCAT GTATTCCGAT GAGATCGAAA TCGAGTCAGC AGATGTTATC 
TCGGTATTGG CCACAGCCAC GTTGTTCCAC TTAGACGGAA TCATCGACAA GTGTGCCGAG 
GTGATGGTGG ATAACATTAG TCCGGAGACA GCTATCCAGT ATTACGAGGC CGCCTGCCAG 
TACGGTGTGG TCGGAGTCAA GAAGTCCACC TTTCAGTGGT TCCAGATCAA TCTGCTAAGC 
ATTTATAGTA AGCAACCGAA CCTGCTTAGG CACATCTCCA TTGAGCTGAT GAGTGCCCTG 
ACCGCCAGTC CCGATTTGTA TGTGATGCAG ACGGAGTTCT CGCTGTACAC TCTACTGCGC 
ACATGGATGT TCCTACGACT GCATCCCGAC TACGATCCAG AGGACCCGGT CCAGCGGGCG 
GAGGCGCTAA AGACGCAGGA GCTTTTGGTT AATGCCGGCG TGGAAACGCA TGCGCCTAGT 
GGTGATGTCG TCCAGTGGAC ATACTTTACC AGCCGCTCGG AGGAACGCTC GTTCTTGGCC 
ACGCCCGAGG GGCAGCCTTA TGTGAAGGTC TTTCAGAAGC TACGAACCCA GTACCTGACC 
AACCACTACA TGGATCTAAA AATCATATAC AATGACAATA TTATACCCAA GGAGTGGCTG 
TATCGGCACA TTCACAACCA CTGGGATGCA TTACTACGTA TCGATCATGG GCAGGAGGAT 
TGTCCTCAGC AGCTGGACGA TGAGCAGTTC TTTGAGAACT GCATGCGATG CGGACGTATG 
TTGCTTGAGC CTGGCTACCA GAAGTGGCGC TGGACGGGCT TCAACTTTGG CATGGATCTC 
ATCCTGATCA TGGACTCGCG TAGACTGAAC ATTCGTCGGC ACCACCGGCA CGAGCACGAG 
CGAGTGCTTA GCCTGCAGAC GAAACGCAAG TTCATGGTCC GCACCACGGT GACCTCGATA 
AACGCCCAGC GCCAGGCAGT CTTCACCCAA ACGTCCGAGA TCTGTTCTCT TAGCCTCGAA 
AAGAACGAGG AGGTGCCACT CATGGTGTTA GACCCAAAGT TGGTGCATCC ACTTCTCATT 
TCAATAAACA TGCTGGTGGT AATGCCACCG AATCAGAGTT TCAAGGAAAT TGTTCCGCTC 
AGCGAGGAGG CGACAACCTC TCTCTCCATA CCCATTTCGG AAATCGGAGC AAACAGCGAC 
AGACCGCTGT CCCCGTCCAG TGCTGATGAT TCGGCCGTTT TCATTGGTGA CTCTGAGCCT 
TCGACGCCAT CCTCGCCAGC CCCACGGCCC AGAATCGCTT GGTCGGCTAG CGAAACGGGC 
GCAATCTGCG GACAACTGGC GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286210.1
CDS	276..1982
Translation	MGQIVGSMHMNVAEVFSNRRKRKRSTDSSLGKDDPAQLDTTQPKKKKLLTTTQYIYKALFKEEKNSDVAVMALDKVWHLHKVYLSQSPYFYTMFNGTWREAQQNFIQITILDDRITVASLDAVFGSMYSDEIEIESADVISVLATATLFHLDGIIDKCAEVMVDNISPETAIQYYEAACQYGVVGVKKSTFQWFQINLLSIYSKQPNLLRHISIELMSALTASPDLYVMQTEFSLYTLLRTWMFLRLHPDYDPEDPVQRAEALKTQELLVNAGVETHAPSGDVVQWTYFTSRSEERSFLATPEGQPYVKVFQKLRTQYLTNHYMDLKIIYNDNIIPKEWLYRHIHNHWDALLRIDHGQEDCPQQLDDEQFFENCMRCGRMLLEPGYQKWRWTGFNFGMDLILIMDSRRLNIRRHHRHEHERVLSLQTKRKFMVRTTVTSINAQRQAVFTQTSEICSLSLEKNEEVPLMVLDPKLVHPLLISINMLVVMPPNQSFKEIVPLSEEATTSLSIPISEIGANSDRPLSPSSADDSAVFIGDSEPSTPSSPAPRPRIAWSASETGAICGQLAC

Target ORF information:

RefSeq Version	NM_001299281.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster germ cell-less (gcl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299281.1

ORF Insert Sequence:

ATGGGTCAAA TAGTGGGATC CATGCATATG AACGTCGCTG AGGTGTTCAG CAATCGACGG 
AAGCGGAAGC GCAGCACTGA TTCATCTTTG GGGAAGGATG ATCCGGCCCA GTTAGACACC 
ACTCAGCCCA AAAAAAAAAA ACTTCTTACT ACCACCCAGT ACATATACAA GGCGCTCTTC 
AAGGAGGAAA AGAACTCGGA TGTGGCTGTC ATGGCTCTGG ATAAGGTCTG GCACCTGCAT 
AAGGTGTACC TGAGCCAGAG TCCATACTTC TATACTATGT TCAATGGGAC GTGGCGGGAA 
GCCCAGCAGA ATTTCATTCA GATCACAATC CTGGACGACC GGATCACCGT TGCTAGCCTG 
GATGCTGTCT TTGGTTCCAT GTATTCCGAT GAGATCGAAA TCGAGTCAGC AGATGTTATC 
TCGGTATTGG CCACAGCCAC GTTGTTCCAC TTAGACGGAA TCATCGACAA GTGTGCCGAG 
GTGATGGTGG ATAACATTAG TCCGGAGACA GCTATCCAGT ATTACGAGGC CGCCTGCCAG 
TACGGTGTGG TCGGAGTCAA GAAGTCCACC TTTCAGTGGT TCCAGATCAA TCTGCTAAGC 
ATTTATAGTA AGCAACCGAA CCTGCTTAGG CACATCTCCA TTGAGCTGAT GAGTGCCCTG 
ACCGCCAGTC CCGATTTGTA TGTGATGCAG ACGGAGTTCT CGCTGTACAC TCTACTGCGC 
ACATGGATGT TCCTACGACT GCATCCCGAC TACGATCCAG AGGACCCGGT CCAGCGGGCG 
GAGGCGCTAA AGACGCAGGA GCTTTTGGTT AATGCCGGCG TGGAAACGCA TGCGCCTAGT 
GGTGATGTCG TCCAGTGGAC ATACTTTACC AGCCGCTCGG AGGAACGCTC GTTCTTGGCC 
ACGCCCGAGG GGCAGCCTTA TGTGAAGGTC TTTCAGAAGC TACGAACCCA GTACCTGACC 
AACCACTACA TGGATCTAAA AATCATATAC AATGACAATA TTATACCCAA GGAGTGGCTG 
TATCGGCACA TTCACAACCA CTGGGATGCA TTACTACGTA TCGATCATGG GCAGGAGGAT 
TGTCCTCAGC AGCTGGACGA TGAGCAGTTC TTTGAGAACT GCATGCGATG CGGACGTATG 
TTGCTTGAGC CTGGCTACCA GAAGTGGCGC TGGACGGGCT TCAACTTTGG CATGGATCTC 
ATCCTGATCA TGGACTCGCG TAGACTGAAC ATTCGTCGGC ACCACCGGCA CGAGCACGAG 
CGAGTGCTTA GCCTGCAGAC GAAACGCAAG TTCATGGTCC GCACCACGGT GACCTCGATA 
AACGCCCAGC GCCAGGCAGT CTTCACCCAA ACGTCCGAGA TCTGTTCTCT TAGCCTCGAA 
AAGAACGAGG AGGTGCCACT CATGGTGTTA GACCCAAAGT TGGTGCATCC ACTTCTCATT 
TCAATAAACA TGCTGGTGGT AATGCCACCG AATCAGAGTT TCAAGGAAAT TGTTCCGCTC 
AGCGAGGAGG CGACAACCTC TCTCTCCATA CCCATTTCGG AAATCGGAGC AAACAGCGAC 
AGACCGCTGT CCCCGTCCAG TGCTGATGAT TCGGCCGTTT TCATTGGTGA CTCTGAGCCT 
TCGACGCCAT CCTCGCCAGC CCCACGGCCC AGAATCGCTT GGTCGGCTAG CGAAACGGGC 
GCAATCTGCG GACAACTGGC GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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