Myof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myof
Entrez Gene ID	309499
Full Name	myoferlin
Synonyms	Fer1l3,RGD1564216
General protein information	Preferred Names myoferlin Names myoferlin fer-1-like 3, myoferlin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231325.3	XP_006231387.1	myoferlin isoform X1
NM_001354115.1	NP_001341044.1	myoferlin
XM_006231326.3	XP_006231388.1	myoferlin isoform X2
NM_001191636.1	NP_001178565.1	myoferlin
XM_008760369.2	XP_008758591.1	myoferlin isoform X3
XM_008760368.2	XP_008758590.1	myoferlin isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	myof	XP_687465.5
	MYOF	XP_001089235.2
Gallus gallus (chicken)	MYOF	XP_001231546.2
Homo sapiens (human)	MYOF	NP_038479.1
Pan troglodytes (chimpanzee)	MYOF	XP_003312803.1
Mus musculus (house mouse)	Myof	NP_001093104.1
Xenopus tropicalis (tropical clawed frog)	myof	NP_001122123.1
Bos taurus (cattle)	MYOF	NP_001180141.1
Rattus norvegicus (Norway rat)	Myof	NP_001178565.1
Canis lupus familiaris (dog)	MYOF	XP_858858.2

Gene Ontology
Bibliography

Related articles in PubMed

Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
Demonbreun AR, Rossi AE, Alvarez MG, Swanson KE, Deveaux HK, Earley JU, Hadhazy M, Vohra R, Walter GA, Pytel P, McNally EM
The American journal of pathology184(1)248-59(2014 Jan)

Protein profile of exosomes from trabecular meshwork cells.
Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL
Journal of proteomics74(6)796-804(2011 May)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
Fujimoto M, Oshima K, Shinkawa T, Wang BB, Inouye S, Hayashida N, Takii R, Nakai A
The Journal of biological chemistry283(44)29961-70(2008 Oct)

Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function.
Bernatchez PN, Acevedo L, Fernandez-Hernando C, Murata T, Chalouni C, Kim J, Erdjument-Bromage H, Shah V, Gratton JP, McNally EM, Tempst P, Sessa WC
The Journal of biological chemistry282(42)30745-53(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Myof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa46754	XM_008760368.2 Latest version!	Rattus norvegicus myoferlin (Myof), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa46754	XM_008760369.2 Latest version!	Rattus norvegicus myoferlin (Myof), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa24057	NM_001354115.1 Latest version!	Rattus norvegicus myoferlin (Myof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa30204	NM_001191636.1 Latest version!	Rattus norvegicus myoferlin (Myof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24056	XM_006231325.3 Latest version!	Rattus norvegicus myoferlin (Myof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24057	XM_006231326.3 Latest version!	Rattus norvegicus myoferlin (Myof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myof ( XM_008760369.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758591.1 Rattus norvegicus myoferlin (Myof), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa46754
Clone ID Related Accession (Same CDS sequence)	XM_008760369.2 , XM_008760368.2
Accession Version	XM_008760369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GATGGAGTCT 
CAAGAGCTGA CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758591.1
CDS	87..5384
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDFSSSGTGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQMESQELTK

Target ORF information:

RefSeq Version	XM_008760369.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760369.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GATGGAGTCT 
CAAGAGCTGA CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( XM_008760368.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758590.1 Rattus norvegicus myoferlin (Myof), transcript variant X3, mRNA.

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CloneID	ORa46754
Clone ID Related Accession (Same CDS sequence)	XM_008760369.2 , XM_008760368.2
Accession Version	XM_008760368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GATGGAGTCT 
CAAGAGCTGA CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758590.1
CDS	87..5384
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDFSSSGTGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQMESQELTK

Target ORF information:

RefSeq Version	XM_008760368.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760368.2

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GATGGAGTCT 
CAAGAGCTGA CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( NM_001354115.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001341044.1 Rattus norvegicus myoferlin (Myof), mRNA.

Price & Availability↑

CloneID	ORa24057
Clone ID Related Accession (Same CDS sequence)	NM_001354115.1 , XM_006231326.3
Accession Version	NM_001354115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105467.7 and AC096364.9. On Aug 5, 2017 this sequence version replaced XM_006231326.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCGATGCGA ATGGAGATAA AGCAGCATCA CCCAATGAGA TGACTTGCCC TCCGGGTTGG 
GAGTGGGAAG ATGACGCATG GACATATGAC ATAAATCGAG CAGTGGACGA GAAAGGCTGG 
GAGTATGGAA TTACCATTCC TCCTGACAAT AAGCCCAAAT CCTGGGTCGC AGCAGAGAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGAAAGC GCAAGAAAGA TTTGACACAG 
ACGGCTTCCA GCACTGCGAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAC 
GCCTCGTTAA TTGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGATAC CTTTCGCCGC 
AGGCGCTGGA GGAGAAAAAT GGCTCCTTCA GAGACACATG GCGCAGCGGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG ATGAAAAGAG CCTAGAGAAG 
CAGAAGCACA GTGCCACCAC TGTGTTTGGA GCAAACACAC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTACGCTGC TACATTTATC AAGCCCGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGACCCGTAT GCTCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATTCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTTGAT 
GACGTTGAAA TCTTTGGGGA GCCCCAAACA GTTCTGCAGA ACCCGCCCAA AGTCATCATC 
GAACTTTTTG ACAGTGACCA AGTGGGCAAA GATGAGTTTT TAGGTCGAAG CATTTGCTCT 
CCTCTGGTGA AACTGAACTC AGAAACAGAC ACCACACCTA AGCTTCTCTG GCACCCAGTT 
ATGAATGGAG ATAAGGCCTG TGGGGATGTC CTTGTAACAG CAGAACTGAT TCTGAGGAAC 
AAGGACGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAAGGGA TCAGACCTGT GGTTCAGCTC ACCGCTATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCC ATCACCTCGC CCAGCCTCGT GGTGGAGTGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTCA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCACCACTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTC GGGCGGAAGC CTGTGGTCGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA CATTGTACCG 
CAACTAAAAG CTTCCCTTAT GTCTGCACCC CCATGCCGGG ATGTCATTAT TGAAATAGAA 
GACACCAAAC CATTGCTGGC TTCTAAGCTG TCAGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCGGGGGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ACGTAGCTGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGTTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCGTCCGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCGG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCCTGGGC AAAAAAGTAA TTGAAGACAG AGACCATTAC 
ATTCCCAACA CTATCAACCC AGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGACA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTGT CCCGGTTCGG GTCTCACTGC 
GGCATCCCTG AGCAGTACTG TGTTTCTGGA GTGAACACCT GGCGAGATCA GCTGAGACCG 
ACACAGTTGC TTCAAAACGT AGCCAGATTC AAAGGCTTCC CGCCACCAGT CCTTTCTGAA 
GATGGAAGCA GGATCCAATA TGGAGGAAGA GACTATCATC TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCT GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGCC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TCTAGGACCA 
CCAGGTCCCC CTTTCAACAT CACTCCCAGG AAAGCCAAGA CGTACTATCT GCGTGTCATC 
ATCTGGAATA CCAGGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGC 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG CGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCCG CTGAGCAGCT CTGTATCGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TCCGAGTGCC CCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTCGACTTGC ATCGTACGAT CATCCCAGCC 
AAGACCTCGG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAGGCTAAGA CTGCTTCTCT CTTTGAACAG AAGTCCATGA AAGGCTGGTG GCCATGCTAC 
GCAGACAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTCCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAAGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGCTGCTGTT CGTGGCTGTG CTCCTGTACT CGCTGCCGAA CTATTTATCC 
ATGAAGATTG TGAGGCCAAA TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341044.1
CDS	87..6233
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKTYYLRVIIWNTRDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQKSMKGWWPCYADKDGSRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	NM_001354115.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354115.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCGATGCGA ATGGAGATAA AGCAGCATCA CCCAATGAGA TGACTTGCCC TCCGGGTTGG 
GAGTGGGAAG ATGACGCATG GACATATGAC ATAAATCGAG CAGTGGACGA GAAAGGCTGG 
GAGTATGGAA TTACCATTCC TCCTGACAAT AAGCCCAAAT CCTGGGTCGC AGCAGAGAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGAAAGC GCAAGAAAGA TTTGACACAG 
ACGGCTTCCA GCACTGCGAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAC 
GCCTCGTTAA TTGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGATAC CTTTCGCCGC 
AGGCGCTGGA GGAGAAAAAT GGCTCCTTCA GAGACACATG GCGCAGCGGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG ATGAAAAGAG CCTAGAGAAG 
CAGAAGCACA GTGCCACCAC TGTGTTTGGA GCAAACACAC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTACGCTGC TACATTTATC AAGCCCGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGACCCGTAT GCTCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATTCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTTGAT 
GACGTTGAAA TCTTTGGGGA GCCCCAAACA GTTCTGCAGA ACCCGCCCAA AGTCATCATC 
GAACTTTTTG ACAGTGACCA AGTGGGCAAA GATGAGTTTT TAGGTCGAAG CATTTGCTCT 
CCTCTGGTGA AACTGAACTC AGAAACAGAC ACCACACCTA AGCTTCTCTG GCACCCAGTT 
ATGAATGGAG ATAAGGCCTG TGGGGATGTC CTTGTAACAG CAGAACTGAT TCTGAGGAAC 
AAGGACGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAAGGGA TCAGACCTGT GGTTCAGCTC ACCGCTATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCC ATCACCTCGC CCAGCCTCGT GGTGGAGTGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTCA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCACCACTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTC GGGCGGAAGC CTGTGGTCGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA CATTGTACCG 
CAACTAAAAG CTTCCCTTAT GTCTGCACCC CCATGCCGGG ATGTCATTAT TGAAATAGAA 
GACACCAAAC CATTGCTGGC TTCTAAGCTG TCAGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCGGGGGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ACGTAGCTGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGTTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCGTCCGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCGG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCCTGGGC AAAAAAGTAA TTGAAGACAG AGACCATTAC 
ATTCCCAACA CTATCAACCC AGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGACA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTGT CCCGGTTCGG GTCTCACTGC 
GGCATCCCTG AGCAGTACTG TGTTTCTGGA GTGAACACCT GGCGAGATCA GCTGAGACCG 
ACACAGTTGC TTCAAAACGT AGCCAGATTC AAAGGCTTCC CGCCACCAGT CCTTTCTGAA 
GATGGAAGCA GGATCCAATA TGGAGGAAGA GACTATCATC TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCT GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGCC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TCTAGGACCA 
CCAGGTCCCC CTTTCAACAT CACTCCCAGG AAAGCCAAGA CGTACTATCT GCGTGTCATC 
ATCTGGAATA CCAGGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGC 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG CGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCCG CTGAGCAGCT CTGTATCGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TCCGAGTGCC CCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTCGACTTGC ATCGTACGAT CATCCCAGCC 
AAGACCTCGG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAGGCTAAGA CTGCTTCTCT CTTTGAACAG AAGTCCATGA AAGGCTGGTG GCCATGCTAC 
GCAGACAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTCCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAAGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGCTGCTGTT CGTGGCTGTG CTCCTGTACT CGCTGCCGAA CTATTTATCC 
ATGAAGATTG TGAGGCCAAA TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( NM_001191636.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178565.1 Rattus norvegicus myoferlin (Myof), mRNA.

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CloneID	ORa30204
Clone ID Related Accession (Same CDS sequence)	NM_001191636.1
Accession Version	NM_001191636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-03-10
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC107608.6, AABR03004966.1 and AABR03000275.1. On Jul 17, 2010 this sequence version replaced XM_220031.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCCGATGCG ACGCGAATGG AGATAAAGCA GCATCACCCA ATGAGATGAC TTGCCCTCCG 
GGTTGGGAGT GGGAAGATGA CGCATGGACA TATGACATAA ATCGAGCAGT GGACGAGAAA 
GGCTGGGAGT ATGGAATTAC CATTCCTCCT GACAATAAGC CCAAATCCTG GGTCGCAGCA 
GAGAAGATGT ATCACACTCA CAGGCGGCGG AGACTGGTCC GAAAGCGCAA GAAAGATTTG 
ACACAGACGG CTTCCAGCAC TGCGAGGGCC ATGGAAGAAC TTGAAGACCG GGAGGGCTGG 
GAATACGCCT CGTTAATTGG CTGGAAATTC CACTGGAAAC AGCGCAGTTC AGATACCTTT 
CGCCGCAGGC GCTGGAGGAG AAAAATGGCT CCTTCAGAGA CACATGGCGC AGCGGCCATC 
TTTAAACTTG AAGGTGCCCT TGGGGCAGAC ACCACAGAGG ATGGAGATGA AAAGAGCCTA 
GAGAAGCAGA AGCACAGTGC CACCACTGTG TTTGGAGCAA ACACACCCAT TGTTTCCTGT 
AATTTCGACA GAGTCTACAT CTACCACCTA CGCTGCTACA TTTATCAAGC CCGGAACCTC 
ATGGCTTTGG ACAAAGACAG CTTTTCAGAC CCGTATGCTC ATGTCTCCTT CCTCCATCGA 
AGCAAAACCA CTGAGATCAT TCACTCCACC CTGAATCCCA CCTGGGACCA AACCATTATA 
TTTGATGACG TTGAAATCTT TGGGGAGCCC CAAACAGTTC TGCAGAACCC GCCCAAAGTC 
ATCATCGAAC TTTTTGACAG TGACCAAGTG GGCAAAGATG AGTTTTTAGG TCGAAGCATT 
TGCTCTCCTC TGGTGAAACT GAACTCAGAA ACAGACACCA CACCTAAGCT TCTCTGGCAC 
CCAGTTATGA ATGGAGATAA GGCCTGTGGG GATGTCCTTG TAACAGCAGA ACTGATTCTG 
AGGAACAAGG ACGGCTCCAA CCTTCCCATC CTCCCCTCTC AAAGAGCACC AAACTTATAC 
ATGGTCCCTC AAGGGATCAG ACCTGTGGTT CAGCTCACCG CTATTGAGAT CCTAGCTTGG 
GGCTTAAGAA ATATGAAAAA CTACCAGATG GCTTCCATCA CCTCGCCCAG CCTCGTGGTG 
GAGTGCGGAG GAGAGAGAGT AGAATCGGTG GTGATCAAAA GCCTCAAGAA GACACCCAAC 
TTCCCAAGTT CTGTTCTCTT CATGAAAGTG TTCTTGCCCA AGGAAGAATT GTACATGCCA 
CCACTGGTGA TCAAGGTCAT TGATCACAGG CAGTTCGGGC GGAAGCCTGT GGTCGGCCAA 
TGCACCATTG ACCACCTGGA CCGCTTTCGG TGTGATCCAT ATGCAGGAAA GGAGGACATT 
GTACCGCAAC TAAAAGCTTC CCTTATGTCT GCACCCCCAT GCCGGGATGT CATTATTGAA 
ATAGAAGACA CCAAACCATT GCTGGCTTCT AAGCTGTCAG AAAAGGAGGA AGAAATCGTT 
GACTGGTGGA GTAAATTTTA TGCTTCCTCG GGGGAGCATG AAAAATGTGG ACAGTATATT 
CAGAAAGGCT ATTCCAAACT CAAGATCTAT GACTGTGAAC TGGAGGACGT AGCTGACTTT 
GAAGGCCTGA CAGATTTCTC AGATACCTTC AAGTTGTACA GAGGCAAGTC CGATGAGAAT 
GAAGATCCGT CCGTGGTAGG AGAGTTTAAA GGCTCCTTCC GAATCTACCC CTTGCCGGAT 
GACCCCAGTG TGCCGGCCCC TCCCAGGCAG TTTCGGGAAC TGCCTGACAG TGTCCCGCAG 
GAATGCACGG TTAGGATTTA CATTGTTCAA GGCTTACAGC TCCAGCCCCA GGACAACAAT 
GGCCTGTGTG ATCCTTACAT AAAAATAACC CTGGGCAAAA AAGTAATTGA AGACAGAGAC 
CATTACATTC CCAACACTAT CAACCCAGTG TTCGGCAGAA TGTATGAACT GAGCTGCTAC 
TTACCTCAAG AGAAAGACCT GAAGATCTCT GTCTATGACT ACGACACCTT CACCCGAGAT 
GAGAAGGTGG GAGAGACAAC CATTGACCTG GAGAACCGCT TCCTGTCCCG GTTCGGGTCT 
CACTGCGGCA TCCCTGAGCA GTACTGTGTT TCTGGAGTGA ACACCTGGCG AGATCAGCTG 
AGACCGACAC AGTTGCTTCA AAACGTAGCC AGATTCAAAG GCTTCCCGCC ACCAGTCCTT 
TCTGAAGATG GAAGCAGGAT CCAATATGGA GGAAGAGACT ATCATCTGGA TGAGTTTGAG 
GCCAACAAAA TCCTGCACCA GCACCTTGGA GCCCCTGAAG AGCGTCTTGC CCTCCATATT 
CTCAGGACTC AGGGCCTGGT CCCTGAGCAC GTGGAGACAA GGACTTTGCA CAGCACCTTC 
CAGCCCAATA TTTCCCAGGG AAAACTTCAG ATGTGGGTGG ATGTCTTCCC CAAGAGTCTA 
GGACCACCAG GTCCCCCTTT CAACATCACT CCCAGGAAAG CCAAGACGTA CTATCTGCGT 
GTCATCATCT GGAATACCAG GGACGTTATC TTGGATGAGA AGAGCATCAC TGGGGAGGAC 
ATGAGCGACA TCTACGTCAA AGGCTGGATT TCCGGCAGCG AAGAAAACAA ACAGAAAACA 
GATGTCCACT ACAGGTCCTT GGATGGCGAA GGCAATTTTA ACTGGAGATT TGTTTTCCCA 
TTTGACTACC TCCCCGCTGA GCAGCTCTGT ATCGTCGCTA AAAAAGAGCA TTTCTGGAGT 
ATTGATCAAA CAGAATTCCG AGTGCCCCCT CGGCTGATCA TTCAGATATG GGACAATGAC 
AAGTTTTCCC TGGACGACTA CTTGGGTTTT CTGGAACTCG ACTTGCATCG TACGATCATC 
CCAGCCAAGA CCTCGGAGAA ATGCAGTTTG GACATGATTC CAGACCTCAA AGCCATGGAC 
CCTCTTAAGG CTAAGACTGC TTCTCTCTTT GAACAGAAGT CCATGAAAGG CTGGTGGCCA 
TGCTACGCAG ACAAAGATGG CTCCCGCGTG ATGGCTGGGA AAGTGGAGAT GACACTGGAG 
GTCCTCAACG AGAGGGAGGC CGACGAGAGG CCAGCCGGGA AAGGGCGGAG TGAACCCAAC 
ATGAACCCCA AGCTTGACCC ACCAAACCGA CCGGAAACCT CCTTCCTCTG GTTCACGAAC 
CCTTGCAAGA CCATGCGCTT CATCGTGTGG CGCCGGTTCA AGTGGGTGAT CATCGGCCTG 
CTTCTGCTGC TCATCCTGCT GCTGTTCGTG GCTGTGCTCC TGTACTCGCT GCCGAACTAT 
TTATCCATGA AGATTGTGAG GCCAAATGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178565.1
CDS	79..6231
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTRCDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKTYYLRVIIWNTRDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQKSMKGWWPCYADKDGSRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	NM_001191636.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191636.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCCGATGCG ACGCGAATGG AGATAAAGCA GCATCACCCA ATGAGATGAC TTGCCCTCCG 
GGTTGGGAGT GGGAAGATGA CGCATGGACA TATGACATAA ATCGAGCAGT GGACGAGAAA 
GGCTGGGAGT ATGGAATTAC CATTCCTCCT GACAATAAGC CCAAATCCTG GGTCGCAGCA 
GAGAAGATGT ATCACACTCA CAGGCGGCGG AGACTGGTCC GAAAGCGCAA GAAAGATTTG 
ACACAGACGG CTTCCAGCAC TGCGAGGGCC ATGGAAGAAC TTGAAGACCG GGAGGGCTGG 
GAATACGCCT CGTTAATTGG CTGGAAATTC CACTGGAAAC AGCGCAGTTC AGATACCTTT 
CGCCGCAGGC GCTGGAGGAG AAAAATGGCT CCTTCAGAGA CACATGGCGC AGCGGCCATC 
TTTAAACTTG AAGGTGCCCT TGGGGCAGAC ACCACAGAGG ATGGAGATGA AAAGAGCCTA 
GAGAAGCAGA AGCACAGTGC CACCACTGTG TTTGGAGCAA ACACACCCAT TGTTTCCTGT 
AATTTCGACA GAGTCTACAT CTACCACCTA CGCTGCTACA TTTATCAAGC CCGGAACCTC 
ATGGCTTTGG ACAAAGACAG CTTTTCAGAC CCGTATGCTC ATGTCTCCTT CCTCCATCGA 
AGCAAAACCA CTGAGATCAT TCACTCCACC CTGAATCCCA CCTGGGACCA AACCATTATA 
TTTGATGACG TTGAAATCTT TGGGGAGCCC CAAACAGTTC TGCAGAACCC GCCCAAAGTC 
ATCATCGAAC TTTTTGACAG TGACCAAGTG GGCAAAGATG AGTTTTTAGG TCGAAGCATT 
TGCTCTCCTC TGGTGAAACT GAACTCAGAA ACAGACACCA CACCTAAGCT TCTCTGGCAC 
CCAGTTATGA ATGGAGATAA GGCCTGTGGG GATGTCCTTG TAACAGCAGA ACTGATTCTG 
AGGAACAAGG ACGGCTCCAA CCTTCCCATC CTCCCCTCTC AAAGAGCACC AAACTTATAC 
ATGGTCCCTC AAGGGATCAG ACCTGTGGTT CAGCTCACCG CTATTGAGAT CCTAGCTTGG 
GGCTTAAGAA ATATGAAAAA CTACCAGATG GCTTCCATCA CCTCGCCCAG CCTCGTGGTG 
GAGTGCGGAG GAGAGAGAGT AGAATCGGTG GTGATCAAAA GCCTCAAGAA GACACCCAAC 
TTCCCAAGTT CTGTTCTCTT CATGAAAGTG TTCTTGCCCA AGGAAGAATT GTACATGCCA 
CCACTGGTGA TCAAGGTCAT TGATCACAGG CAGTTCGGGC GGAAGCCTGT GGTCGGCCAA 
TGCACCATTG ACCACCTGGA CCGCTTTCGG TGTGATCCAT ATGCAGGAAA GGAGGACATT 
GTACCGCAAC TAAAAGCTTC CCTTATGTCT GCACCCCCAT GCCGGGATGT CATTATTGAA 
ATAGAAGACA CCAAACCATT GCTGGCTTCT AAGCTGTCAG AAAAGGAGGA AGAAATCGTT 
GACTGGTGGA GTAAATTTTA TGCTTCCTCG GGGGAGCATG AAAAATGTGG ACAGTATATT 
CAGAAAGGCT ATTCCAAACT CAAGATCTAT GACTGTGAAC TGGAGGACGT AGCTGACTTT 
GAAGGCCTGA CAGATTTCTC AGATACCTTC AAGTTGTACA GAGGCAAGTC CGATGAGAAT 
GAAGATCCGT CCGTGGTAGG AGAGTTTAAA GGCTCCTTCC GAATCTACCC CTTGCCGGAT 
GACCCCAGTG TGCCGGCCCC TCCCAGGCAG TTTCGGGAAC TGCCTGACAG TGTCCCGCAG 
GAATGCACGG TTAGGATTTA CATTGTTCAA GGCTTACAGC TCCAGCCCCA GGACAACAAT 
GGCCTGTGTG ATCCTTACAT AAAAATAACC CTGGGCAAAA AAGTAATTGA AGACAGAGAC 
CATTACATTC CCAACACTAT CAACCCAGTG TTCGGCAGAA TGTATGAACT GAGCTGCTAC 
TTACCTCAAG AGAAAGACCT GAAGATCTCT GTCTATGACT ACGACACCTT CACCCGAGAT 
GAGAAGGTGG GAGAGACAAC CATTGACCTG GAGAACCGCT TCCTGTCCCG GTTCGGGTCT 
CACTGCGGCA TCCCTGAGCA GTACTGTGTT TCTGGAGTGA ACACCTGGCG AGATCAGCTG 
AGACCGACAC AGTTGCTTCA AAACGTAGCC AGATTCAAAG GCTTCCCGCC ACCAGTCCTT 
TCTGAAGATG GAAGCAGGAT CCAATATGGA GGAAGAGACT ATCATCTGGA TGAGTTTGAG 
GCCAACAAAA TCCTGCACCA GCACCTTGGA GCCCCTGAAG AGCGTCTTGC CCTCCATATT 
CTCAGGACTC AGGGCCTGGT CCCTGAGCAC GTGGAGACAA GGACTTTGCA CAGCACCTTC 
CAGCCCAATA TTTCCCAGGG AAAACTTCAG ATGTGGGTGG ATGTCTTCCC CAAGAGTCTA 
GGACCACCAG GTCCCCCTTT CAACATCACT CCCAGGAAAG CCAAGACGTA CTATCTGCGT 
GTCATCATCT GGAATACCAG GGACGTTATC TTGGATGAGA AGAGCATCAC TGGGGAGGAC 
ATGAGCGACA TCTACGTCAA AGGCTGGATT TCCGGCAGCG AAGAAAACAA ACAGAAAACA 
GATGTCCACT ACAGGTCCTT GGATGGCGAA GGCAATTTTA ACTGGAGATT TGTTTTCCCA 
TTTGACTACC TCCCCGCTGA GCAGCTCTGT ATCGTCGCTA AAAAAGAGCA TTTCTGGAGT 
ATTGATCAAA CAGAATTCCG AGTGCCCCCT CGGCTGATCA TTCAGATATG GGACAATGAC 
AAGTTTTCCC TGGACGACTA CTTGGGTTTT CTGGAACTCG ACTTGCATCG TACGATCATC 
CCAGCCAAGA CCTCGGAGAA ATGCAGTTTG GACATGATTC CAGACCTCAA AGCCATGGAC 
CCTCTTAAGG CTAAGACTGC TTCTCTCTTT GAACAGAAGT CCATGAAAGG CTGGTGGCCA 
TGCTACGCAG ACAAAGATGG CTCCCGCGTG ATGGCTGGGA AAGTGGAGAT GACACTGGAG 
GTCCTCAACG AGAGGGAGGC CGACGAGAGG CCAGCCGGGA AAGGGCGGAG TGAACCCAAC 
ATGAACCCCA AGCTTGACCC ACCAAACCGA CCGGAAACCT CCTTCCTCTG GTTCACGAAC 
CCTTGCAAGA CCATGCGCTT CATCGTGTGG CGCCGGTTCA AGTGGGTGAT CATCGGCCTG 
CTTCTGCTGC TCATCCTGCT GCTGTTCGTG GCTGTGCTCC TGTACTCGCT GCCGAACTAT 
TTATCCATGA AGATTGTGAG GCCAAATGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( XM_006231325.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231387.1 Rattus norvegicus myoferlin (Myof), transcript variant X1, mRNA.

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CloneID	ORa24056
Clone ID Related Accession (Same CDS sequence)	XM_006231325.3
Accession Version	XM_006231325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTCTT CCCCAAGAGT CTAGGACCAC CAGGTCCCCC TTTCAACATC 
ACTCCCAGGA AAGCCAAGAC GTACTATCTG CGTGTCATCA TCTGGAATAC CAGGGACGTT 
ATCTTGGATG AGAAGAGCAT CACTGGGGAG GACATGAGCG ACATCTACGT CAAAGGCTGG 
ATTTCCGGCA GCGAAGAAAA CAAACAGAAA ACAGATGTCC ACTACAGGTC CTTGGATGGC 
GAAGGCAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCCGC TGAGCAGCTC 
TGTATCGTCG CTAAAAAAGA GCATTTCTGG AGTATTGATC AAACAGAATT CCGAGTGCCC 
CCTCGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CCCTGGACGA CTACTTGGGT 
TTTCTGGAAC TCGACTTGCA TCGTACGATC ATCCCAGCCA AGACCTCGGA GAAATGCAGT 
TTGGACATGA TTCCAGACCT CAAAGCCATG GACCCTCTTA AGGCTAAGAC TGCTTCTCTC 
TTTGAACAGA AGTCCATGAA AGGCTGGTGG CCATGCTACG CAGACAAAGA TGGCTCCCGC 
GTGATGGCTG GGAAAGTGGA GATGACACTG GAGGTCCTCA ACGAGAGGGA GGCCGACGAG 
AGGCCAGCCG GGAAAGGGCG GAGTGAACCC AACATGAACC CCAAGCTTGA CCCACCAAAC 
CGACCGGAAA CCTCCTTCCT CTGGTTCACG AACCCTTGCA AGACCATGCG CTTCATCGTG 
TGGCGCCGGT TCAAGTGGGT GATCATCGGC CTGCTTCTGC TGCTCATCCT GCTGCTGTTC 
GTGGCTGTGC TCCTGTACTC GCTGCCGAAC TATTTATCCA TGAAGATTGT GAGGCCAAAT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231387.1
CDS	87..6272
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDFSSSGTGAASYTANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKTYYLRVIIWNTRDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQKSMKGWWPCYADKDGSRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	XM_006231325.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231325.3

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAACTGGG 
GCTGCGTCAT ATACAGCAAA TACAGGAGAG ACTGAGGTGG GTTTCGTCCC AACATTTGGA 
CCTTGTTATT TGAACCTTTA TGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAC 
GATGAGCTGA ATTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTGGTTGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TTGAGCCCAT TTCAAATGAT 
GACCTTCTGG TTGTTGAGAA ATACCAGCGA CGGCGGAAGT ACAGCCTGTC GGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTCTGG CATCAACAAC TCAATACAGC 
CGTGCAGTAT TTGATGGGAA CTACTATTAC TATTTACCTT GGGCCCGCAC AAAGCCTGTT 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATCGAGGCTG TAAAATCAGG AATCCAAGGT 
AAAATTCCCA CAAACCAGTT GGCAGAGCTG TGGCTGAAGC TAATAGACGA AGTTATAGAA 
GACACAAGAT ACACCTTGCC TGTCACTGAA GGAAAAGCCA ACGTCACAGT TCTGGATACT 
CAGATCCGAA AGTTGCGGCT GAGGTTTCTC TCGCAGATAC ACGAGGCGGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGCTGGAGA TCGAGGAGTG GCTTGATAAA 
TTAATGCAAC TTACTGAAGA GCCTCAGAAC AGTATGCCCG ACATCATCAT CTGGATGATC 
CGCGGGGAGA AAAGACTGGC TTATGCACGG ATTCCTGCCC ACCAAGTCTT ATACTCCACC 
AGTGGTGGGA ACGCCTCCGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AGAACAATGG GCCCAAAGTG CCTGTGGAAT TGCGAGTGAA CATCTGGCTG 
GGTTTAAGCG CCGTGGAAAA GAAGTTCAAT AGCTTCGCTG AAGGCACATT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTCGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAAAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAATG GGAAGGAGAC TGGGTAGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ATGCAGGTCA CACAGAGTTC ACTGATGAAG TGTACCAGAA CGAGACTCGC 
TATCCTGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CCGATGCGAA TGGAGATAAA 
GCAGCATCAC CCAATGAGAT GACTTGCCCT CCGGGTTGGG AGTGGGAAGA TGACGCATGG 
ACATATGACA TAAATCGAGC AGTGGACGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT 
CCTGACAATA AGCCCAAATC CTGGGTCGCA GCAGAGAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGAAAGCG CAAGAAAGAT TTGACACAGA CGGCTTCCAG CACTGCGAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATACG CCTCGTTAAT TGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGATACC TTTCGCCGCA GGCGCTGGAG GAGAAAAATG 
GCTCCTTCAG AGACACATGG CGCAGCGGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA TGAAAAGAGC CTAGAGAAGC AGAAGCACAG TGCCACCACT 
GTGTTTGGAG CAAACACACC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTACGCTGCT ACATTTATCA AGCCCGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GACCCGTATG CTCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATTCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTTGATG ACGTTGAAAT CTTTGGGGAG 
CCCCAAACAG TTCTGCAGAA CCCGCCCAAA GTCATCATCG AACTTTTTGA CAGTGACCAA 
GTGGGCAAAG ATGAGTTTTT AGGTCGAAGC ATTTGCTCTC CTCTGGTGAA ACTGAACTCA 
GAAACAGACA CCACACCTAA GCTTCTCTGG CACCCAGTTA TGAATGGAGA TAAGGCCTGT 
GGGGATGTCC TTGTAACAGC AGAACTGATT CTGAGGAACA AGGACGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAAGGGAT CAGACCTGTG 
GTTCAGCTCA CCGCTATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCCA TCACCTCGCC CAGCCTCGTG GTGGAGTGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTCAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCACCACTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTCG GGCGGAAGCC TGTGGTCGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAC ATTGTACCGC AACTAAAAGC TTCCCTTATG 
TCTGCACCCC CATGCCGGGA TGTCATTATT GAAATAGAAG ACACCAAACC ATTGCTGGCT 
TCTAAGCTGT CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCGGGGGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA CGTAGCTGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGTTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CGTCCGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCGGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCCTGGGCA AAAAAGTAAT TGAAGACAGA GACCATTACA TTCCCAACAC TATCAACCCA 
GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGACAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTGTC CCGGTTCGGG TCTCACTGCG GCATCCCTGA GCAGTACTGT 
GTTTCTGGAG TGAACACCTG GCGAGATCAG CTGAGACCGA CACAGTTGCT TCAAAACGTA 
GCCAGATTCA AAGGCTTCCC GCCACCAGTC CTTTCTGAAG ATGGAAGCAG GATCCAATAT 
GGAGGAAGAG ACTATCATCT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCTG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGCCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTCTT CCCCAAGAGT CTAGGACCAC CAGGTCCCCC TTTCAACATC 
ACTCCCAGGA AAGCCAAGAC GTACTATCTG CGTGTCATCA TCTGGAATAC CAGGGACGTT 
ATCTTGGATG AGAAGAGCAT CACTGGGGAG GACATGAGCG ACATCTACGT CAAAGGCTGG 
ATTTCCGGCA GCGAAGAAAA CAAACAGAAA ACAGATGTCC ACTACAGGTC CTTGGATGGC 
GAAGGCAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCCGC TGAGCAGCTC 
TGTATCGTCG CTAAAAAAGA GCATTTCTGG AGTATTGATC AAACAGAATT CCGAGTGCCC 
CCTCGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CCCTGGACGA CTACTTGGGT 
TTTCTGGAAC TCGACTTGCA TCGTACGATC ATCCCAGCCA AGACCTCGGA GAAATGCAGT 
TTGGACATGA TTCCAGACCT CAAAGCCATG GACCCTCTTA AGGCTAAGAC TGCTTCTCTC 
TTTGAACAGA AGTCCATGAA AGGCTGGTGG CCATGCTACG CAGACAAAGA TGGCTCCCGC 
GTGATGGCTG GGAAAGTGGA GATGACACTG GAGGTCCTCA ACGAGAGGGA GGCCGACGAG 
AGGCCAGCCG GGAAAGGGCG GAGTGAACCC AACATGAACC CCAAGCTTGA CCCACCAAAC 
CGACCGGAAA CCTCCTTCCT CTGGTTCACG AACCCTTGCA AGACCATGCG CTTCATCGTG 
TGGCGCCGGT TCAAGTGGGT GATCATCGGC CTGCTTCTGC TGCTCATCCT GCTGCTGTTC 
GTGGCTGTGC TCCTGTACTC GCTGCCGAAC TATTTATCCA TGAAGATTGT GAGGCCAAAT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( XM_006231326.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231388.1 Rattus norvegicus myoferlin (Myof), transcript variant X2, mRNA.

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CloneID	ORa24057
Clone ID Related Accession (Same CDS sequence)	NM_001354115.1 , XM_006231326.3
Accession Version	XM_006231326.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myoferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCGATGCGA ATGGAGATAA AGCAGCATCA CCCAATGAGA TGACTTGCCC TCCGGGTTGG 
GAGTGGGAAG ATGACGCATG GACATATGAC ATAAATCGAG CAGTGGACGA GAAAGGCTGG 
GAGTATGGAA TTACCATTCC TCCTGACAAT AAGCCCAAAT CCTGGGTCGC AGCAGAGAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGAAAGC GCAAGAAAGA TTTGACACAG 
ACGGCTTCCA GCACTGCGAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAC 
GCCTCGTTAA TTGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGATAC CTTTCGCCGC 
AGGCGCTGGA GGAGAAAAAT GGCTCCTTCA GAGACACATG GCGCAGCGGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG ATGAAAAGAG CCTAGAGAAG 
CAGAAGCACA GTGCCACCAC TGTGTTTGGA GCAAACACAC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTACGCTGC TACATTTATC AAGCCCGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGACCCGTAT GCTCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATTCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTTGAT 
GACGTTGAAA TCTTTGGGGA GCCCCAAACA GTTCTGCAGA ACCCGCCCAA AGTCATCATC 
GAACTTTTTG ACAGTGACCA AGTGGGCAAA GATGAGTTTT TAGGTCGAAG CATTTGCTCT 
CCTCTGGTGA AACTGAACTC AGAAACAGAC ACCACACCTA AGCTTCTCTG GCACCCAGTT 
ATGAATGGAG ATAAGGCCTG TGGGGATGTC CTTGTAACAG CAGAACTGAT TCTGAGGAAC 
AAGGACGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAAGGGA TCAGACCTGT GGTTCAGCTC ACCGCTATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCC ATCACCTCGC CCAGCCTCGT GGTGGAGTGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTCA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCACCACTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTC GGGCGGAAGC CTGTGGTCGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA CATTGTACCG 
CAACTAAAAG CTTCCCTTAT GTCTGCACCC CCATGCCGGG ATGTCATTAT TGAAATAGAA 
GACACCAAAC CATTGCTGGC TTCTAAGCTG TCAGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCGGGGGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ACGTAGCTGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGTTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCGTCCGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCGG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCCTGGGC AAAAAAGTAA TTGAAGACAG AGACCATTAC 
ATTCCCAACA CTATCAACCC AGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGACA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTGT CCCGGTTCGG GTCTCACTGC 
GGCATCCCTG AGCAGTACTG TGTTTCTGGA GTGAACACCT GGCGAGATCA GCTGAGACCG 
ACACAGTTGC TTCAAAACGT AGCCAGATTC AAAGGCTTCC CGCCACCAGT CCTTTCTGAA 
GATGGAAGCA GGATCCAATA TGGAGGAAGA GACTATCATC TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCT GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGCC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TCTAGGACCA 
CCAGGTCCCC CTTTCAACAT CACTCCCAGG AAAGCCAAGA CGTACTATCT GCGTGTCATC 
ATCTGGAATA CCAGGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGC 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG CGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCCG CTGAGCAGCT CTGTATCGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TCCGAGTGCC CCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTCGACTTGC ATCGTACGAT CATCCCAGCC 
AAGACCTCGG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAGGCTAAGA CTGCTTCTCT CTTTGAACAG AAGTCCATGA AAGGCTGGTG GCCATGCTAC 
GCAGACAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTCCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAAGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGCTGCTGTT CGTGGCTGTG CTCCTGTACT CGCTGCCGAA CTATTTATCC 
ATGAAGATTG TGAGGCCAAA TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231388.1
CDS	87..6233
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQISLLNEKGQDTGATIDLVIGYTPPSAPYPNDPSGTSVTGVGEEEEEDQGDEDRVDGMVRGPGPKGPSGTMSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADSLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPSEKRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEANTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRILVELNTFLEKKPPEKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWARTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPTNQLAELWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRLRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNETRYPGGEWKQAEDTYTDANGDKAASPNEMTCPPGWEWEDDAWTYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDDVEIFGEPQTVLQNPPKVIIELFDSDQVGKDEFLGRSICSPLVKLNSETDTTPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASITSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCRDVIIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTINPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIQYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKTYYLRVIIWNTRDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQKSMKGWWPCYADKDGSRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	XM_006231326.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myoferlin (Myof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231326.3

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCTACT AACATCCCTA AAACGAAATT TGGGAAACCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCTT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CGATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTGTC CCTGAAGGAC CTGATCGGGG ACCAGAACAG ATCTCTGCCC 
TACAAACAGA TCTCCCTGCT AAACGAGAAG GGACAAGACA CGGGGGCCAC CATTGACCTG 
GTGATTGGCT ATACCCCACC TTCAGCTCCA TATCCAAATG ATCCGAGTGG GACCAGTGTG 
ACAGGCGTGG GAGAAGAAGA GGAAGAAGAC CAGGGGGATG AAGACAGGGT AGATGGCATG 
GTCAGAGGCC CTGGACCCAA GGGGCCATCT GGGACAATGT CAGAAGCTCA GCTTGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGGAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATTGA AGGCCGTCAG TTATGCGGCA ACAACATAAG GCCTGTGGTG 
AAAGTTCACG TCTGTGGTCA GACACACCGC ACGAGAATCA AGAGAGGGAA CAATCCATTT 
TTTGATGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAACTCAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCCCACTCT CTGAGGGCAG ATAGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTACGATGAA CCTGGCCATG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGTGCAAAGG GGTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCA TCTGAGAAAC GAGACCGGGA CAATGACAGT 
GATGACGTCG AGAGCAACTT GTTACTTCCA GCTGGCATTG CCCTCCGGTG GGTAACCTTC 
TTGCTGAAGA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTGAAGGAAA TATTTGGTGG CAATGCTGAT AAAAAAAATC TCGTGGACCC TTTCGTAGAG 
GTCTCCTTTG CTGGGAAAAA GGTGTGTACA AATATTATTG AGAGAAATGC AAACCCCGAA 
TGGAATCAAG TTGTCAATCT TCAGATCAAG TTTCCTTCCA TGTGTGAAAA AATAAAACTA 
ACAGTGTATG ACTGGGACCG GCTTACCAAA AATGATGTAG TTGGGACGAC ATACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA ATACAGGAGA GACTGAGGTG 
GGTTTCGTCC CAACATTTGG ACCTTGTTAT TTGAACCTTT ATGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA CGATGAGCTG AATTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTGGTTGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTTGAGCCCA TTTCAAATGA TGACCTTCTG GTTGTTGAGA AATACCAGCG ACGGCGGAAG 
TACAGCCTGT CGGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTCTG 
GCATCAACAA CTCAATACAG CCGTGCAGTA TTTGATGGGA ACTACTATTA CTATTTACCT 
TGGGCCCGCA CAAAGCCTGT TGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCATCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATCGAGGCT 
GTAAAATCAG GAATCCAAGG TAAAATTCCC ACAAACCAGT TGGCAGAGCT GTGGCTGAAG 
CTAATAGACG AAGTTATAGA AGACACAAGA TACACCTTGC CTGTCACTGA AGGAAAAGCC 
AACGTCACAG TTCTGGATAC TCAGATCCGA AAGTTGCGGC TGAGGTTTCT CTCGCAGATA 
CACGAGGCGG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGCTGGAG 
ATCGAGGAGT GGCTTGATAA ATTAATGCAA CTTACTGAAG AGCCTCAGAA CAGTATGCCC 
GACATCATCA TCTGGATGAT CCGCGGGGAG AAAAGACTGG CTTATGCACG GATTCCTGCC 
CACCAAGTCT TATACTCCAC CAGTGGTGGG AACGCCTCCG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAGAACAATG GGCCCAAAGT GCCTGTGGAA 
TTGCGAGTGA ACATCTGGCT GGGTTTAAGC GCCGTGGAAA AGAAGTTCAA TAGCTTCGCT 
GAAGGCACAT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTCGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAAAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAAT GGGAAGGAGA CTGGGTAGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GATGCAGGTC ACACAGAGTT CACTGATGAA 
GTGTACCAGA ACGAGACTCG CTATCCTGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACCGATGCGA ATGGAGATAA AGCAGCATCA CCCAATGAGA TGACTTGCCC TCCGGGTTGG 
GAGTGGGAAG ATGACGCATG GACATATGAC ATAAATCGAG CAGTGGACGA GAAAGGCTGG 
GAGTATGGAA TTACCATTCC TCCTGACAAT AAGCCCAAAT CCTGGGTCGC AGCAGAGAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGAAAGC GCAAGAAAGA TTTGACACAG 
ACGGCTTCCA GCACTGCGAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAC 
GCCTCGTTAA TTGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGATAC CTTTCGCCGC 
AGGCGCTGGA GGAGAAAAAT GGCTCCTTCA GAGACACATG GCGCAGCGGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG ATGAAAAGAG CCTAGAGAAG 
CAGAAGCACA GTGCCACCAC TGTGTTTGGA GCAAACACAC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTACGCTGC TACATTTATC AAGCCCGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGACCCGTAT GCTCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATTCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTTGAT 
GACGTTGAAA TCTTTGGGGA GCCCCAAACA GTTCTGCAGA ACCCGCCCAA AGTCATCATC 
GAACTTTTTG ACAGTGACCA AGTGGGCAAA GATGAGTTTT TAGGTCGAAG CATTTGCTCT 
CCTCTGGTGA AACTGAACTC AGAAACAGAC ACCACACCTA AGCTTCTCTG GCACCCAGTT 
ATGAATGGAG ATAAGGCCTG TGGGGATGTC CTTGTAACAG CAGAACTGAT TCTGAGGAAC 
AAGGACGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAAGGGA TCAGACCTGT GGTTCAGCTC ACCGCTATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCC ATCACCTCGC CCAGCCTCGT GGTGGAGTGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTCA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCACCACTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTC GGGCGGAAGC CTGTGGTCGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA CATTGTACCG 
CAACTAAAAG CTTCCCTTAT GTCTGCACCC CCATGCCGGG ATGTCATTAT TGAAATAGAA 
GACACCAAAC CATTGCTGGC TTCTAAGCTG TCAGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCGGGGGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ACGTAGCTGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGTTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCGTCCGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCGG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC GCAGGAATGC 
ACGGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCCTGGGC AAAAAAGTAA TTGAAGACAG AGACCATTAC 
ATTCCCAACA CTATCAACCC AGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGACA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTGT CCCGGTTCGG GTCTCACTGC 
GGCATCCCTG AGCAGTACTG TGTTTCTGGA GTGAACACCT GGCGAGATCA GCTGAGACCG 
ACACAGTTGC TTCAAAACGT AGCCAGATTC AAAGGCTTCC CGCCACCAGT CCTTTCTGAA 
GATGGAAGCA GGATCCAATA TGGAGGAAGA GACTATCATC TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCT GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGCC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TCTAGGACCA 
CCAGGTCCCC CTTTCAACAT CACTCCCAGG AAAGCCAAGA CGTACTATCT GCGTGTCATC 
ATCTGGAATA CCAGGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGC 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG CGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCCG CTGAGCAGCT CTGTATCGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TCCGAGTGCC CCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTCGACTTGC ATCGTACGAT CATCCCAGCC 
AAGACCTCGG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAGGCTAAGA CTGCTTCTCT CTTTGAACAG AAGTCCATGA AAGGCTGGTG GCCATGCTAC 
GCAGACAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTCCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAAGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGCTGCTGTT CGTGGCTGTG CTCCTGTACT CGCTGCCGAA CTATTTATCC 
ATGAAGATTG TGAGGCCAAA TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
The American journal of pathology184(1)248-59(2014 Jan)
Demonbreun AR,Rossi AE,Alvarez MG,Swanson KE,Deveaux HK,Earley JU,Hadhazy M,Vohra R,Walter GA,Pytel P,McNally EM

Protein profile of exosomes from trabecular meshwork cells.
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Myof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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