myof.1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following myof.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myof.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00896 NM_001128651.1
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa61084 XM_031904680.1
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61085 XM_012965820.3
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61086 XM_031904681.1
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61087 XM_012965821.3
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61088 XM_031904683.1
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61089 XM_031904684.1
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61090 XM_012965825.3
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa61091 XM_012965826.3
Latest version!
Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa29938 XM_012965820.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa29939 XM_012965821.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa29940 XM_012965822.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa29941 XM_012965823.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa29942 XM_012965825.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa29943 XM_012965826.2
Latest version!
Xenopus tropicalis myoferlin (myof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa00896
    Clone ID Related Accession (Same CDS sequence) NM_001128651.1
    Accession Version NM_001128651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167451.1. ##Evidence-Data-START## Transcript exon combination :: BC167451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028299, SAMD00028306 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT 
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001122123.1
    CDS475..6264
    Translation

    Target ORF information:

    RefSeq Version NM_001128651.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT 
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61084
    Clone ID Related Accession (Same CDS sequence) XM_031904680.1
    Accession Version XM_031904680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGGGA
    TCTTCTGGCA TTGGGATATT AGGCGGGAAA AATCTGTCGT TGTTAAGCAG TATGTCAACA
    GCAATCGGAA AAATGGCTTC TCCAACTACA GTGCATCTAC AAGAAAAGGA GGAAGAAGTA
    GTGGACTGGT GGAGCAAATA CTATGCTTCA ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT
    ATTCAGAAAG GATACACCAC CTTAAAGGTC TATAAATGTG AGCTGGAGAA TGTTTCTGAG
    TTCCGTGGCC TTACAGACTT CTGTGACACA TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC
    AGTGATGACC CTTCTGTTGT AGGAGAGTTT AAAGGTTCCT TCCGTATCTA CCCACTTCCA
    GATGATCCAA ACATTCCATA TCCTCCTCGA CAATTCCTAG AATTGCCAGG AACTGAATCT
    CAGGAGTGTA TTGTTCGTAT ATACATTGTG AGAGGCATAG ACCTGCAACC AAAGGATAAC
    AATGGACTGT GTGACCCATA TATTAAAATT ACATTGAATA AAAAGGTGAT TGAAGATCGA
    GATCACTACA TACCAAATAC ACTGAACCCC TTATTTGGCA GAATGTATGA ACTCAGCTGC
    TTCCTACCTC AAGAAAAGGA CCTCAAGATC TCTGTGTATG ATTATGATAC ATTAACCCGG
    GATGAAAAAG TCGGCGAAAC AACAATAGAT CTGGAGAACA GATTCCTCTC TCGGTTTGGA
    AGCCATTGTG GCTTGCCACA GACTTACTGC ATCTCAGGAA TAAACCAATG GAGGGACCAG
    CTGACACCTA CCCAAATCCT ACAAAACTTC GCCAGATTAA AATCCTCTCC TCCCCCTGTG
    TTTTCAGACA ATGGAACAAG ACTTACTTTT TCCAGCAAAG ACTATACATT AGAGGAATTT
    GAAAATAATA GAAAGATTCA CCAGCATCTC GGACCACCAA ACGAGCGTCT TGCTCTGTAT
    GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG CATGTTGAAA CTAGAACTTT GTATAGCACT
    TTCCAGCCAA ACATTTCCCA AGGAAAACTA GAAATGTGGG TTGATGTTTT CCCAAAAAGT
    TTAGGACCTC CAGGGCCACC TTTCAACATT ACTCCACGCA AAGCAAAGAA GTATGTACTT
    CGTGTTATTG TTTGGAATAC CAAAGATGTC ATTCTAGATG AGAAAAGTAT TACTGGAGAA
    GAAATGAGTG ATATTTACGT GAAGGGGTGG ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA
    ACCGACGTAC ACTACAGGTC ACTGGATGGG GAAGGAAACT TTAATTGGAG ATTTGTTTTT
    CCATTTGAAT ATCTTCCAGC CGAACAGTTG TGCATTGTTT CTAAGAAGGA ACATTTCTGG
    AGCCTTGACA AAACGGAATT TAAGCTCCCA CCCAAACTAA TTCTTCAAAT ATGGGACAAT
    GATAAATTCT CCTTGGATGA CTACTTAGGT TTTGTAGAAC TTGATTTACA TCGAACAACA
    ATCCCTGCGA AAGTCCCAGA AAAATGCAGT TTTAACTTAC TTGATCAAGA CAAGCATTCA
    AAGGTGGCCT CTCTGTTTGA GCAGAAATCC ATGAAGGGAT GGTGGCCATG CCATGCAGAG
    AAGGATGGGA AAAGGATTTT AGCTGGGAAA ATTGAGATGA CTCTTGAGGT CCTGAATGAA
    AAAGATGCAG AAGAAAGGCC TGCTGGCAAG GGACGAGATG AGCCCAACAT GAACCCCAAG
    TTAGACCCAC CAAACCGACC AGACACCTCC TTCCTCTGGT TTACAAACCC ATGCAAGACA
    ATGAAATTTA TCATATGGCG CAGATTTAAA TGGGTGTTTA TTGGACTCAT CATCCTGCTG
    CTTGTACTTC TGTTCCTTGG AGTTTTCTTC TATTCTTTGC CGGGCTATGT TTCTATGAAG
    ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760540.1
    CDS152..6412
    Translation

    Target ORF information:

    RefSeq Version XM_031904680.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGGGA
    TCTTCTGGCA TTGGGATATT AGGCGGGAAA AATCTGTCGT TGTTAAGCAG TATGTCAACA
    GCAATCGGAA AAATGGCTTC TCCAACTACA GTGCATCTAC AAGAAAAGGA GGAAGAAGTA
    GTGGACTGGT GGAGCAAATA CTATGCTTCA ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT
    ATTCAGAAAG GATACACCAC CTTAAAGGTC TATAAATGTG AGCTGGAGAA TGTTTCTGAG
    TTCCGTGGCC TTACAGACTT CTGTGACACA TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC
    AGTGATGACC CTTCTGTTGT AGGAGAGTTT AAAGGTTCCT TCCGTATCTA CCCACTTCCA
    GATGATCCAA ACATTCCATA TCCTCCTCGA CAATTCCTAG AATTGCCAGG AACTGAATCT
    CAGGAGTGTA TTGTTCGTAT ATACATTGTG AGAGGCATAG ACCTGCAACC AAAGGATAAC
    AATGGACTGT GTGACCCATA TATTAAAATT ACATTGAATA AAAAGGTGAT TGAAGATCGA
    GATCACTACA TACCAAATAC ACTGAACCCC TTATTTGGCA GAATGTATGA ACTCAGCTGC
    TTCCTACCTC AAGAAAAGGA CCTCAAGATC TCTGTGTATG ATTATGATAC ATTAACCCGG
    GATGAAAAAG TCGGCGAAAC AACAATAGAT CTGGAGAACA GATTCCTCTC TCGGTTTGGA
    AGCCATTGTG GCTTGCCACA GACTTACTGC ATCTCAGGAA TAAACCAATG GAGGGACCAG
    CTGACACCTA CCCAAATCCT ACAAAACTTC GCCAGATTAA AATCCTCTCC TCCCCCTGTG
    TTTTCAGACA ATGGAACAAG ACTTACTTTT TCCAGCAAAG ACTATACATT AGAGGAATTT
    GAAAATAATA GAAAGATTCA CCAGCATCTC GGACCACCAA ACGAGCGTCT TGCTCTGTAT
    GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG CATGTTGAAA CTAGAACTTT GTATAGCACT
    TTCCAGCCAA ACATTTCCCA AGGAAAACTA GAAATGTGGG TTGATGTTTT CCCAAAAAGT
    TTAGGACCTC CAGGGCCACC TTTCAACATT ACTCCACGCA AAGCAAAGAA GTATGTACTT
    CGTGTTATTG TTTGGAATAC CAAAGATGTC ATTCTAGATG AGAAAAGTAT TACTGGAGAA
    GAAATGAGTG ATATTTACGT GAAGGGGTGG ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA
    ACCGACGTAC ACTACAGGTC ACTGGATGGG GAAGGAAACT TTAATTGGAG ATTTGTTTTT
    CCATTTGAAT ATCTTCCAGC CGAACAGTTG TGCATTGTTT CTAAGAAGGA ACATTTCTGG
    AGCCTTGACA AAACGGAATT TAAGCTCCCA CCCAAACTAA TTCTTCAAAT ATGGGACAAT
    GATAAATTCT CCTTGGATGA CTACTTAGGT TTTGTAGAAC TTGATTTACA TCGAACAACA
    ATCCCTGCGA AAGTCCCAGA AAAATGCAGT TTTAACTTAC TTGATCAAGA CAAGCATTCA
    AAGGTGGCCT CTCTGTTTGA GCAGAAATCC ATGAAGGGAT GGTGGCCATG CCATGCAGAG
    AAGGATGGGA AAAGGATTTT AGCTGGGAAA ATTGAGATGA CTCTTGAGGT CCTGAATGAA
    AAAGATGCAG AAGAAAGGCC TGCTGGCAAG GGACGAGATG AGCCCAACAT GAACCCCAAG
    TTAGACCCAC CAAACCGACC AGACACCTCC TTCCTCTGGT TTACAAACCC ATGCAAGACA
    ATGAAATTTA TCATATGGCG CAGATTTAAA TGGGTGTTTA TTGGACTCAT CATCCTGCTG
    CTTGTACTTC TGTTCCTTGG AGTTTTCTTC TATTCTTTGC CGGGCTATGT TTCTATGAAG
    ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61085
    Clone ID Related Accession (Same CDS sequence) XM_012965820.3
    Accession Version XM_012965820.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGTTG TTAAGCAGTA TGTCAACAGC AATCGGAAAA
    ATGGCTTCTC CAACTACAGT GCATCTACAA GAAAAGGAGG AAGAAGTAGT GGACTGGTGG
    AGCAAATACT ATGCTTCAAC TGGAGAAACT GAGAAGTGTG GGCAGTATAT TCAGAAAGGA
    TACACCACCT TAAAGGTCTA TAAATGTGAG CTGGAGAATG TTTCTGAGTT CCGTGGCCTT
    ACAGACTTCT GTGACACATT CAAGCTGTAC AGAGGAAAAG CAGAAGACAG TGATGACCCT
    TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC CGTATCTACC CACTTCCAGA TGATCCAAAC
    ATTCCATATC CTCCTCGACA ATTCCTAGAA TTGCCAGGAA CTGAATCTCA GGAGTGTATT
    GTTCGTATAT ACATTGTGAG AGGCATAGAC CTGCAACCAA AGGATAACAA TGGACTGTGT
    GACCCATATA TTAAAATTAC ATTGAATAAA AAGGTGATTG AAGATCGAGA TCACTACATA
    CCAAATACAC TGAACCCCTT ATTTGGCAGA ATGTATGAAC TCAGCTGCTT CCTACCTCAA
    GAAAAGGACC TCAAGATCTC TGTGTATGAT TATGATACAT TAACCCGGGA TGAAAAAGTC
    GGCGAAACAA CAATAGATCT GGAGAACAGA TTCCTCTCTC GGTTTGGAAG CCATTGTGGC
    TTGCCACAGA CTTACTGCAT CTCAGGAATA AACCAATGGA GGGACCAGCT GACACCTACC
    CAAATCCTAC AAAACTTCGC CAGATTAAAA TCCTCTCCTC CCCCTGTGTT TTCAGACAAT
    GGAACAAGAC TTACTTTTTC CAGCAAAGAC TATACATTAG AGGAATTTGA AAATAATAGA
    AAGATTCACC AGCATCTCGG ACCACCAAAC GAGCGTCTTG CTCTGTATGT CCTTAGAACT
    CAAGGGCTTG TTCCAGAGCA TGTTGAAACT AGAACTTTGT ATAGCACTTT CCAGCCAAAC
    ATTTCCCAAG GAAAACTAGA AATGTGGGTT GATGTTTTCC CAAAAAGTTT AGGACCTCCA
    GGGCCACCTT TCAACATTAC TCCACGCAAA GCAAAGAAGT ATGTACTTCG TGTTATTGTT
    TGGAATACCA AAGATGTCAT TCTAGATGAG AAAAGTATTA CTGGAGAAGA AATGAGTGAT
    ATTTACGTGA AGGGGTGGAT TCCTGGCAAT GAAGAAAACA AGCAGAAAAC CGACGTACAC
    TACAGGTCAC TGGATGGGGA AGGAAACTTT AATTGGAGAT TTGTTTTTCC ATTTGAATAT
    CTTCCAGCCG AACAGTTGTG CATTGTTTCT AAGAAGGAAC ATTTCTGGAG CCTTGACAAA
    ACGGAATTTA AGCTCCCACC CAAACTAATT CTTCAAATAT GGGACAATGA TAAATTCTCC
    TTGGATGACT ACTTAGGTTT TGTAGAACTT GATTTACATC GAACAACAAT CCCTGCGAAA
    GTCCCAGAAA AATGCAGTTT TAACTTACTT GATCAAGACA AGCATTCAAA GGTGGCCTCT
    CTGTTTGAGC AGAAATCCAT GAAGGGATGG TGGCCATGCC ATGCAGAGAA GGATGGGAAA
    AGGATTTTAG CTGGGAAAAT TGAGATGACT CTTGAGGTCC TGAATGAAAA AGATGCAGAA
    GAAAGGCCTG CTGGCAAGGG ACGAGATGAG CCCAACATGA ACCCCAAGTT AGACCCACCA
    AACCGACCAG ACACCTCCTT CCTCTGGTTT ACAAACCCAT GCAAGACAAT GAAATTTATC
    ATATGGCGCA GATTTAAATG GGTGTTTATT GGACTCATCA TCCTGCTGCT TGTACTTCTG
    TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG GGCTATGTTT CTATGAAGAT TGTGAAACCC
    AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821274.1
    CDS152..6400
    Translation

    Target ORF information:

    RefSeq Version XM_012965820.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965820.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGTTG TTAAGCAGTA TGTCAACAGC AATCGGAAAA
    ATGGCTTCTC CAACTACAGT GCATCTACAA GAAAAGGAGG AAGAAGTAGT GGACTGGTGG
    AGCAAATACT ATGCTTCAAC TGGAGAAACT GAGAAGTGTG GGCAGTATAT TCAGAAAGGA
    TACACCACCT TAAAGGTCTA TAAATGTGAG CTGGAGAATG TTTCTGAGTT CCGTGGCCTT
    ACAGACTTCT GTGACACATT CAAGCTGTAC AGAGGAAAAG CAGAAGACAG TGATGACCCT
    TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC CGTATCTACC CACTTCCAGA TGATCCAAAC
    ATTCCATATC CTCCTCGACA ATTCCTAGAA TTGCCAGGAA CTGAATCTCA GGAGTGTATT
    GTTCGTATAT ACATTGTGAG AGGCATAGAC CTGCAACCAA AGGATAACAA TGGACTGTGT
    GACCCATATA TTAAAATTAC ATTGAATAAA AAGGTGATTG AAGATCGAGA TCACTACATA
    CCAAATACAC TGAACCCCTT ATTTGGCAGA ATGTATGAAC TCAGCTGCTT CCTACCTCAA
    GAAAAGGACC TCAAGATCTC TGTGTATGAT TATGATACAT TAACCCGGGA TGAAAAAGTC
    GGCGAAACAA CAATAGATCT GGAGAACAGA TTCCTCTCTC GGTTTGGAAG CCATTGTGGC
    TTGCCACAGA CTTACTGCAT CTCAGGAATA AACCAATGGA GGGACCAGCT GACACCTACC
    CAAATCCTAC AAAACTTCGC CAGATTAAAA TCCTCTCCTC CCCCTGTGTT TTCAGACAAT
    GGAACAAGAC TTACTTTTTC CAGCAAAGAC TATACATTAG AGGAATTTGA AAATAATAGA
    AAGATTCACC AGCATCTCGG ACCACCAAAC GAGCGTCTTG CTCTGTATGT CCTTAGAACT
    CAAGGGCTTG TTCCAGAGCA TGTTGAAACT AGAACTTTGT ATAGCACTTT CCAGCCAAAC
    ATTTCCCAAG GAAAACTAGA AATGTGGGTT GATGTTTTCC CAAAAAGTTT AGGACCTCCA
    GGGCCACCTT TCAACATTAC TCCACGCAAA GCAAAGAAGT ATGTACTTCG TGTTATTGTT
    TGGAATACCA AAGATGTCAT TCTAGATGAG AAAAGTATTA CTGGAGAAGA AATGAGTGAT
    ATTTACGTGA AGGGGTGGAT TCCTGGCAAT GAAGAAAACA AGCAGAAAAC CGACGTACAC
    TACAGGTCAC TGGATGGGGA AGGAAACTTT AATTGGAGAT TTGTTTTTCC ATTTGAATAT
    CTTCCAGCCG AACAGTTGTG CATTGTTTCT AAGAAGGAAC ATTTCTGGAG CCTTGACAAA
    ACGGAATTTA AGCTCCCACC CAAACTAATT CTTCAAATAT GGGACAATGA TAAATTCTCC
    TTGGATGACT ACTTAGGTTT TGTAGAACTT GATTTACATC GAACAACAAT CCCTGCGAAA
    GTCCCAGAAA AATGCAGTTT TAACTTACTT GATCAAGACA AGCATTCAAA GGTGGCCTCT
    CTGTTTGAGC AGAAATCCAT GAAGGGATGG TGGCCATGCC ATGCAGAGAA GGATGGGAAA
    AGGATTTTAG CTGGGAAAAT TGAGATGACT CTTGAGGTCC TGAATGAAAA AGATGCAGAA
    GAAAGGCCTG CTGGCAAGGG ACGAGATGAG CCCAACATGA ACCCCAAGTT AGACCCACCA
    AACCGACCAG ACACCTCCTT CCTCTGGTTT ACAAACCCAT GCAAGACAAT GAAATTTATC
    ATATGGCGCA GATTTAAATG GGTGTTTATT GGACTCATCA TCCTGCTGCT TGTACTTCTG
    TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG GGCTATGTTT CTATGAAGAT TGTGAAACCC
    AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61086
    Clone ID Related Accession (Same CDS sequence) XM_031904681.1
    Accession Version XM_031904681.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGTTG
    TTAAGCAGTA TGTCAACAGC AATCGGAAAA ATGGCTTCTC CAACTACAGT GCATCTACAA
    GAAAAGGAGG AAGAAGTAGT GGACTGGTGG AGCAAATACT ATGCTTCAAC TGGAGAAACT
    GAGAAGTGTG GGCAGTATAT TCAGAAAGGA TACACCACCT TAAAGGTCTA TAAATGTGAG
    CTGGAGAATG TTTCTGAGTT CCGTGGCCTT ACAGACTTCT GTGACACATT CAAGCTGTAC
    AGAGGAAAAG CAGAAGACAG TGATGACCCT TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC
    CGTATCTACC CACTTCCAGA TGATCCAAAC ATTCCATATC CTCCTCGACA ATTCCTAGAA
    TTGCCAGGAA CTGAATCTCA GGAGTGTATT GTTCGTATAT ACATTGTGAG AGGCATAGAC
    CTGCAACCAA AGGATAACAA TGGACTGTGT GACCCATATA TTAAAATTAC ATTGAATAAA
    AAGGTGATTG AAGATCGAGA TCACTACATA CCAAATACAC TGAACCCCTT ATTTGGCAGA
    ATGTATGAAC TCAGCTGCTT CCTACCTCAA GAAAAGGACC TCAAGATCTC TGTGTATGAT
    TATGATACAT TAACCCGGGA TGAAAAAGTC GGCGAAACAA CAATAGATCT GGAGAACAGA
    TTCCTCTCTC GGTTTGGAAG CCATTGTGGC TTGCCACAGA CTTACTGCAT CTCAGGAATA
    AACCAATGGA GGGACCAGCT GACACCTACC CAAATCCTAC AAAACTTCGC CAGATTAAAA
    TCCTCTCCTC CCCCTGTGTT TTCAGACAAT GGAACAAGAC TTACTTTTTC CAGCAAAGAC
    TATACATTAG AGGAATTTGA AAATAATAGA AAGATTCACC AGCATCTCGG ACCACCAAAC
    GAGCGTCTTG CTCTGTATGT CCTTAGAACT CAAGGGCTTG TTCCAGAGCA TGTTGAAACT
    AGAACTTTGT ATAGCACTTT CCAGCCAAAC ATTTCCCAAG GAAAACTAGA AATGTGGGTT
    GATGTTTTCC CAAAAAGTTT AGGACCTCCA GGGCCACCTT TCAACATTAC TCCACGCAAA
    GCAAAGAAGT ATGTACTTCG TGTTATTGTT TGGAATACCA AAGATGTCAT TCTAGATGAG
    AAAAGTATTA CTGGAGAAGA AATGAGTGAT ATTTACGTGA AGGGGTGGAT TCCTGGCAAT
    GAAGAAAACA AGCAGAAAAC CGACGTACAC TACAGGTCAC TGGATGGGGA AGGAAACTTT
    AATTGGAGAT TTGTTTTTCC ATTTGAATAT CTTCCAGCCG AACAGTTGTG CATTGTTTCT
    AAGAAGGAAC ATTTCTGGAG CCTTGACAAA ACGGAATTTA AGCTCCCACC CAAACTAATT
    CTTCAAATAT GGGACAATGA TAAATTCTCC TTGGATGACT ACTTAGGTTT TGTAGAACTT
    GATTTACATC GAACAACAAT CCCTGCGAAA GTCCCAGAAA AATGCAGTTT TAACTTACTT
    GATCAAGACA AGCATTCAAA GGTGGCCTCT CTGTTTGAGC AGAAATCCAT GAAGGGATGG
    TGGCCATGCC ATGCAGAGAA GGATGGGAAA AGGATTTTAG CTGGGAAAAT TGAGATGACT
    CTTGAGGTCC TGAATGAAAA AGATGCAGAA GAAAGGCCTG CTGGCAAGGG ACGAGATGAG
    CCCAACATGA ACCCCAAGTT AGACCCACCA AACCGACCAG ACACCTCCTT CCTCTGGTTT
    ACAAACCCAT GCAAGACAAT GAAATTTATC ATATGGCGCA GATTTAAATG GGTGTTTATT
    GGACTCATCA TCCTGCTGCT TGTACTTCTG TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG
    GGCTATGTTT CTATGAAGAT TGTGAAACCC AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760541.1
    CDS152..6370
    Translation

    Target ORF information:

    RefSeq Version XM_031904681.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGTTG
    TTAAGCAGTA TGTCAACAGC AATCGGAAAA ATGGCTTCTC CAACTACAGT GCATCTACAA
    GAAAAGGAGG AAGAAGTAGT GGACTGGTGG AGCAAATACT ATGCTTCAAC TGGAGAAACT
    GAGAAGTGTG GGCAGTATAT TCAGAAAGGA TACACCACCT TAAAGGTCTA TAAATGTGAG
    CTGGAGAATG TTTCTGAGTT CCGTGGCCTT ACAGACTTCT GTGACACATT CAAGCTGTAC
    AGAGGAAAAG CAGAAGACAG TGATGACCCT TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC
    CGTATCTACC CACTTCCAGA TGATCCAAAC ATTCCATATC CTCCTCGACA ATTCCTAGAA
    TTGCCAGGAA CTGAATCTCA GGAGTGTATT GTTCGTATAT ACATTGTGAG AGGCATAGAC
    CTGCAACCAA AGGATAACAA TGGACTGTGT GACCCATATA TTAAAATTAC ATTGAATAAA
    AAGGTGATTG AAGATCGAGA TCACTACATA CCAAATACAC TGAACCCCTT ATTTGGCAGA
    ATGTATGAAC TCAGCTGCTT CCTACCTCAA GAAAAGGACC TCAAGATCTC TGTGTATGAT
    TATGATACAT TAACCCGGGA TGAAAAAGTC GGCGAAACAA CAATAGATCT GGAGAACAGA
    TTCCTCTCTC GGTTTGGAAG CCATTGTGGC TTGCCACAGA CTTACTGCAT CTCAGGAATA
    AACCAATGGA GGGACCAGCT GACACCTACC CAAATCCTAC AAAACTTCGC CAGATTAAAA
    TCCTCTCCTC CCCCTGTGTT TTCAGACAAT GGAACAAGAC TTACTTTTTC CAGCAAAGAC
    TATACATTAG AGGAATTTGA AAATAATAGA AAGATTCACC AGCATCTCGG ACCACCAAAC
    GAGCGTCTTG CTCTGTATGT CCTTAGAACT CAAGGGCTTG TTCCAGAGCA TGTTGAAACT
    AGAACTTTGT ATAGCACTTT CCAGCCAAAC ATTTCCCAAG GAAAACTAGA AATGTGGGTT
    GATGTTTTCC CAAAAAGTTT AGGACCTCCA GGGCCACCTT TCAACATTAC TCCACGCAAA
    GCAAAGAAGT ATGTACTTCG TGTTATTGTT TGGAATACCA AAGATGTCAT TCTAGATGAG
    AAAAGTATTA CTGGAGAAGA AATGAGTGAT ATTTACGTGA AGGGGTGGAT TCCTGGCAAT
    GAAGAAAACA AGCAGAAAAC CGACGTACAC TACAGGTCAC TGGATGGGGA AGGAAACTTT
    AATTGGAGAT TTGTTTTTCC ATTTGAATAT CTTCCAGCCG AACAGTTGTG CATTGTTTCT
    AAGAAGGAAC ATTTCTGGAG CCTTGACAAA ACGGAATTTA AGCTCCCACC CAAACTAATT
    CTTCAAATAT GGGACAATGA TAAATTCTCC TTGGATGACT ACTTAGGTTT TGTAGAACTT
    GATTTACATC GAACAACAAT CCCTGCGAAA GTCCCAGAAA AATGCAGTTT TAACTTACTT
    GATCAAGACA AGCATTCAAA GGTGGCCTCT CTGTTTGAGC AGAAATCCAT GAAGGGATGG
    TGGCCATGCC ATGCAGAGAA GGATGGGAAA AGGATTTTAG CTGGGAAAAT TGAGATGACT
    CTTGAGGTCC TGAATGAAAA AGATGCAGAA GAAAGGCCTG CTGGCAAGGG ACGAGATGAG
    CCCAACATGA ACCCCAAGTT AGACCCACCA AACCGACCAG ACACCTCCTT CCTCTGGTTT
    ACAAACCCAT GCAAGACAAT GAAATTTATC ATATGGCGCA GATTTAAATG GGTGTTTATT
    GGACTCATCA TCCTGCTGCT TGTACTTCTG TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG
    GGCTATGTTT CTATGAAGAT TGTGAAACCC AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61087
    Clone ID Related Accession (Same CDS sequence) XM_012965821.3
    Accession Version XM_012965821.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCCGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGGGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGGGAT CTTCTGGCAT TGGGATATTA GGCGGGAAAA ATCTGTCGTT GTTAAGCAGT
    ATGTCAACAG CAATCGGAAA AATGGCTTCT CCAACTACAG TGCATCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821275.1
    CDS152..6361
    Translation

    Target ORF information:

    RefSeq Version XM_012965821.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965821.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCCGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGGGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGGGAT CTTCTGGCAT TGGGATATTA GGCGGGAAAA ATCTGTCGTT GTTAAGCAGT
    ATGTCAACAG CAATCGGAAA AATGGCTTCT CCAACTACAG TGCATCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61088
    Clone ID Related Accession (Same CDS sequence) XM_031904683.1
    Accession Version XM_031904683.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGGGA
    TCTTCTGGCA TTGGGATATT AGGCGGGAAA AATCTGTCGC TACAAGAAAA GGAGGAAGAA
    GTAGTGGACT GGTGGAGCAA ATACTATGCT TCAACTGGAG AAACTGAGAA GTGTGGGCAG
    TATATTCAGA AAGGATACAC CACCTTAAAG GTCTATAAAT GTGAGCTGGA GAATGTTTCT
    GAGTTCCGTG GCCTTACAGA CTTCTGTGAC ACATTCAAGC TGTACAGAGG AAAAGCAGAA
    GACAGTGATG ACCCTTCTGT TGTAGGAGAG TTTAAAGGTT CCTTCCGTAT CTACCCACTT
    CCAGATGATC CAAACATTCC ATATCCTCCT CGACAATTCC TAGAATTGCC AGGAACTGAA
    TCTCAGGAGT GTATTGTTCG TATATACATT GTGAGAGGCA TAGACCTGCA ACCAAAGGAT
    AACAATGGAC TGTGTGACCC ATATATTAAA ATTACATTGA ATAAAAAGGT GATTGAAGAT
    CGAGATCACT ACATACCAAA TACACTGAAC CCCTTATTTG GCAGAATGTA TGAACTCAGC
    TGCTTCCTAC CTCAAGAAAA GGACCTCAAG ATCTCTGTGT ATGATTATGA TACATTAACC
    CGGGATGAAA AAGTCGGCGA AACAACAATA GATCTGGAGA ACAGATTCCT CTCTCGGTTT
    GGAAGCCATT GTGGCTTGCC ACAGACTTAC TGCATCTCAG GAATAAACCA ATGGAGGGAC
    CAGCTGACAC CTACCCAAAT CCTACAAAAC TTCGCCAGAT TAAAATCCTC TCCTCCCCCT
    GTGTTTTCAG ACAATGGAAC AAGACTTACT TTTTCCAGCA AAGACTATAC ATTAGAGGAA
    TTTGAAAATA ATAGAAAGAT TCACCAGCAT CTCGGACCAC CAAACGAGCG TCTTGCTCTG
    TATGTCCTTA GAACTCAAGG GCTTGTTCCA GAGCATGTTG AAACTAGAAC TTTGTATAGC
    ACTTTCCAGC CAAACATTTC CCAAGGAAAA CTAGAAATGT GGGTTGATGT TTTCCCAAAA
    AGTTTAGGAC CTCCAGGGCC ACCTTTCAAC ATTACTCCAC GCAAAGCAAA GAAGTATGTA
    CTTCGTGTTA TTGTTTGGAA TACCAAAGAT GTCATTCTAG ATGAGAAAAG TATTACTGGA
    GAAGAAATGA GTGATATTTA CGTGAAGGGG TGGATTCCTG GCAATGAAGA AAACAAGCAG
    AAAACCGACG TACACTACAG GTCACTGGAT GGGGAAGGAA ACTTTAATTG GAGATTTGTT
    TTTCCATTTG AATATCTTCC AGCCGAACAG TTGTGCATTG TTTCTAAGAA GGAACATTTC
    TGGAGCCTTG ACAAAACGGA ATTTAAGCTC CCACCCAAAC TAATTCTTCA AATATGGGAC
    AATGATAAAT TCTCCTTGGA TGACTACTTA GGTTTTGTAG AACTTGATTT ACATCGAACA
    ACAATCCCTG CGAAAGTCCC AGAAAAATGC AGTTTTAACT TACTTGATCA AGACAAGCAT
    TCAAAGGTGG CCTCTCTGTT TGAGCAGAAA TCCATGAAGG GATGGTGGCC ATGCCATGCA
    GAGAAGGATG GGAAAAGGAT TTTAGCTGGG AAAATTGAGA TGACTCTTGA GGTCCTGAAT
    GAAAAAGATG CAGAAGAAAG GCCTGCTGGC AAGGGACGAG ATGAGCCCAA CATGAACCCC
    AAGTTAGACC CACCAAACCG ACCAGACACC TCCTTCCTCT GGTTTACAAA CCCATGCAAG
    ACAATGAAAT TTATCATATG GCGCAGATTT AAATGGGTGT TTATTGGACT CATCATCCTG
    CTGCTTGTAC TTCTGTTCCT TGGAGTTTTC TTCTATTCTT TGCCGGGCTA TGTTTCTATG
    AAGATTGTGA AACCCAATCT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760543.1
    CDS152..6355
    Translation

    Target ORF information:

    RefSeq Version XM_031904683.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904683.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGGGA
    TCTTCTGGCA TTGGGATATT AGGCGGGAAA AATCTGTCGC TACAAGAAAA GGAGGAAGAA
    GTAGTGGACT GGTGGAGCAA ATACTATGCT TCAACTGGAG AAACTGAGAA GTGTGGGCAG
    TATATTCAGA AAGGATACAC CACCTTAAAG GTCTATAAAT GTGAGCTGGA GAATGTTTCT
    GAGTTCCGTG GCCTTACAGA CTTCTGTGAC ACATTCAAGC TGTACAGAGG AAAAGCAGAA
    GACAGTGATG ACCCTTCTGT TGTAGGAGAG TTTAAAGGTT CCTTCCGTAT CTACCCACTT
    CCAGATGATC CAAACATTCC ATATCCTCCT CGACAATTCC TAGAATTGCC AGGAACTGAA
    TCTCAGGAGT GTATTGTTCG TATATACATT GTGAGAGGCA TAGACCTGCA ACCAAAGGAT
    AACAATGGAC TGTGTGACCC ATATATTAAA ATTACATTGA ATAAAAAGGT GATTGAAGAT
    CGAGATCACT ACATACCAAA TACACTGAAC CCCTTATTTG GCAGAATGTA TGAACTCAGC
    TGCTTCCTAC CTCAAGAAAA GGACCTCAAG ATCTCTGTGT ATGATTATGA TACATTAACC
    CGGGATGAAA AAGTCGGCGA AACAACAATA GATCTGGAGA ACAGATTCCT CTCTCGGTTT
    GGAAGCCATT GTGGCTTGCC ACAGACTTAC TGCATCTCAG GAATAAACCA ATGGAGGGAC
    CAGCTGACAC CTACCCAAAT CCTACAAAAC TTCGCCAGAT TAAAATCCTC TCCTCCCCCT
    GTGTTTTCAG ACAATGGAAC AAGACTTACT TTTTCCAGCA AAGACTATAC ATTAGAGGAA
    TTTGAAAATA ATAGAAAGAT TCACCAGCAT CTCGGACCAC CAAACGAGCG TCTTGCTCTG
    TATGTCCTTA GAACTCAAGG GCTTGTTCCA GAGCATGTTG AAACTAGAAC TTTGTATAGC
    ACTTTCCAGC CAAACATTTC CCAAGGAAAA CTAGAAATGT GGGTTGATGT TTTCCCAAAA
    AGTTTAGGAC CTCCAGGGCC ACCTTTCAAC ATTACTCCAC GCAAAGCAAA GAAGTATGTA
    CTTCGTGTTA TTGTTTGGAA TACCAAAGAT GTCATTCTAG ATGAGAAAAG TATTACTGGA
    GAAGAAATGA GTGATATTTA CGTGAAGGGG TGGATTCCTG GCAATGAAGA AAACAAGCAG
    AAAACCGACG TACACTACAG GTCACTGGAT GGGGAAGGAA ACTTTAATTG GAGATTTGTT
    TTTCCATTTG AATATCTTCC AGCCGAACAG TTGTGCATTG TTTCTAAGAA GGAACATTTC
    TGGAGCCTTG ACAAAACGGA ATTTAAGCTC CCACCCAAAC TAATTCTTCA AATATGGGAC
    AATGATAAAT TCTCCTTGGA TGACTACTTA GGTTTTGTAG AACTTGATTT ACATCGAACA
    ACAATCCCTG CGAAAGTCCC AGAAAAATGC AGTTTTAACT TACTTGATCA AGACAAGCAT
    TCAAAGGTGG CCTCTCTGTT TGAGCAGAAA TCCATGAAGG GATGGTGGCC ATGCCATGCA
    GAGAAGGATG GGAAAAGGAT TTTAGCTGGG AAAATTGAGA TGACTCTTGA GGTCCTGAAT
    GAAAAAGATG CAGAAGAAAG GCCTGCTGGC AAGGGACGAG ATGAGCCCAA CATGAACCCC
    AAGTTAGACC CACCAAACCG ACCAGACACC TCCTTCCTCT GGTTTACAAA CCCATGCAAG
    ACAATGAAAT TTATCATATG GCGCAGATTT AAATGGGTGT TTATTGGACT CATCATCCTG
    CTGCTTGTAC TTCTGTTCCT TGGAGTTTTC TTCTATTCTT TGCCGGGCTA TGTTTCTATG
    AAGATTGTGA AACCCAATCT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61089
    Clone ID Related Accession (Same CDS sequence) XM_031904684.1
    Accession Version XM_031904684.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGCTA
    CAAGAAAAGG AGGAAGAAGT AGTGGACTGG TGGAGCAAAT ACTATGCTTC AACTGGAGAA
    ACTGAGAAGT GTGGGCAGTA TATTCAGAAA GGATACACCA CCTTAAAGGT CTATAAATGT
    GAGCTGGAGA ATGTTTCTGA GTTCCGTGGC CTTACAGACT TCTGTGACAC ATTCAAGCTG
    TACAGAGGAA AAGCAGAAGA CAGTGATGAC CCTTCTGTTG TAGGAGAGTT TAAAGGTTCC
    TTCCGTATCT ACCCACTTCC AGATGATCCA AACATTCCAT ATCCTCCTCG ACAATTCCTA
    GAATTGCCAG GAACTGAATC TCAGGAGTGT ATTGTTCGTA TATACATTGT GAGAGGCATA
    GACCTGCAAC CAAAGGATAA CAATGGACTG TGTGACCCAT ATATTAAAAT TACATTGAAT
    AAAAAGGTGA TTGAAGATCG AGATCACTAC ATACCAAATA CACTGAACCC CTTATTTGGC
    AGAATGTATG AACTCAGCTG CTTCCTACCT CAAGAAAAGG ACCTCAAGAT CTCTGTGTAT
    GATTATGATA CATTAACCCG GGATGAAAAA GTCGGCGAAA CAACAATAGA TCTGGAGAAC
    AGATTCCTCT CTCGGTTTGG AAGCCATTGT GGCTTGCCAC AGACTTACTG CATCTCAGGA
    ATAAACCAAT GGAGGGACCA GCTGACACCT ACCCAAATCC TACAAAACTT CGCCAGATTA
    AAATCCTCTC CTCCCCCTGT GTTTTCAGAC AATGGAACAA GACTTACTTT TTCCAGCAAA
    GACTATACAT TAGAGGAATT TGAAAATAAT AGAAAGATTC ACCAGCATCT CGGACCACCA
    AACGAGCGTC TTGCTCTGTA TGTCCTTAGA ACTCAAGGGC TTGTTCCAGA GCATGTTGAA
    ACTAGAACTT TGTATAGCAC TTTCCAGCCA AACATTTCCC AAGGAAAACT AGAAATGTGG
    GTTGATGTTT TCCCAAAAAG TTTAGGACCT CCAGGGCCAC CTTTCAACAT TACTCCACGC
    AAAGCAAAGA AGTATGTACT TCGTGTTATT GTTTGGAATA CCAAAGATGT CATTCTAGAT
    GAGAAAAGTA TTACTGGAGA AGAAATGAGT GATATTTACG TGAAGGGGTG GATTCCTGGC
    AATGAAGAAA ACAAGCAGAA AACCGACGTA CACTACAGGT CACTGGATGG GGAAGGAAAC
    TTTAATTGGA GATTTGTTTT TCCATTTGAA TATCTTCCAG CCGAACAGTT GTGCATTGTT
    TCTAAGAAGG AACATTTCTG GAGCCTTGAC AAAACGGAAT TTAAGCTCCC ACCCAAACTA
    ATTCTTCAAA TATGGGACAA TGATAAATTC TCCTTGGATG ACTACTTAGG TTTTGTAGAA
    CTTGATTTAC ATCGAACAAC AATCCCTGCG AAAGTCCCAG AAAAATGCAG TTTTAACTTA
    CTTGATCAAG ACAAGCATTC AAAGGTGGCC TCTCTGTTTG AGCAGAAATC CATGAAGGGA
    TGGTGGCCAT GCCATGCAGA GAAGGATGGG AAAAGGATTT TAGCTGGGAA AATTGAGATG
    ACTCTTGAGG TCCTGAATGA AAAAGATGCA GAAGAAAGGC CTGCTGGCAA GGGACGAGAT
    GAGCCCAACA TGAACCCCAA GTTAGACCCA CCAAACCGAC CAGACACCTC CTTCCTCTGG
    TTTACAAACC CATGCAAGAC AATGAAATTT ATCATATGGC GCAGATTTAA ATGGGTGTTT
    ATTGGACTCA TCATCCTGCT GCTTGTACTT CTGTTCCTTG GAGTTTTCTT CTATTCTTTG
    CCGGGCTATG TTTCTATGAA GATTGTGAAA CCCAATCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760544.1
    CDS152..6313
    Translation

    Target ORF information:

    RefSeq Version XM_031904684.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904684.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGGTCTATA TGTTGCAACC ACAGAAAAGG AAGTAGGGTT TCTGCCAACA
    TTTGGACCTT GCTACTTGAA TCTTTATGGA AGCCCAAGAG AATATACAGG GTTCCCAGAT
    CCCTATGATG ACCTGAATTT TGGGAAGGGG GAAGGTGTTG CTTATAGAGG ACGTGTTTTG
    GTGGAACTCA CTACTAAACT GGATAACTCC TCAATTAAAA AGATTGAAGA CATTTCTAGT
    GATGACATCC TTGTTGTTGA GAAATATCAG CGCAGACGAA AATACTGTTT ATGTGCAGTT
    TTCCATTCAG CCACCATGAT ACAAGACATT GGTGAAGCTA TACAGTTTGA AGTCAGCATT
    GGCAATTATG GCAACAAATT TGATAGCACT TGTAAACCAC TGGCATCTAC AACCCAATAC
    AGCCGTCCCA TATTTGATGG CAACTATTAT TACTACCTAC CCTGGTCATT CACAAAGCCT
    GTTGTAACAT TAACTTCTTA CTGGGAAGAC ATAAGCCATC GACTGGACAT AGTTAATATT
    CTTATAGCCA TGACAGATCG ACTGCAATCC AATATATCCA CACTGAAATC TGCAATACAG
    GCAAAGCTTC CTGATGTACG TCTTGCAGAA ATATGGATGA GGCTTATTGA TCAACTTATT
    GAAGATACGA TGAGGCCAAT GCCATCCCTG GAAGGAAAAG CCAATGTTAC AGTGCTGGAT
    AAGCAGCGCG ATAAACTTCG GCAGACCTCC CTCAAATACA TACAGGAGGC AGCTATTAAA
    ATGAGGGGGG AAGCAACAGA TGTGAAAGCA ACACTGACGG AGATTGAGGA CTGGTTGGAT
    CGATTACAGC AGCTGTCTGA AGAGCCACAG AACAGCATGC CCGACGTGAT CATCTGGATG
    ATTCGAGCAG AGAAGAGACT TGCCTACGCA CGTGTCCCAG CTCATCAGGT TCTCTTTTCC
    AAAACCAGCG AGGAAGCCTG TGGAAAATAC TGCGGAAAGA CCCAAACTGT TTTTTTACAG
    TACCCACTTG ACAAGACCAA AGGTTTAAAA ATTCCTACTG AGCTACGAGT GAACATCTGG
    CTGGGACTGT CGGAAGTTGA AAAGAAGTTT AACAGCTATT CAGAAGGAAC CTTTAGTGTT
    TATGCTGAAA TGTATGAAAA TCAAGCTCTC TTACTTGGGA AATGGGGCAC CACAGGTCTT
    CTCAAGCGCC ACAAATTTTC TGATGTCACT GGCTCAATAA AGCTAAAGAG AGAAAGCTTT
    TTGCCCCCAA AGGGATGGGA ATGGGAAGAT GACTGGAAAG TGGACCCTGA AAGGAGCTTA
    CTAACTGAAG CCGATGCTGG TCATACTGAG TTTACAGATG AAATCTTTGA AAATGAGGCT
    CGCTATCCAG GTGGTGAATG GAAGAAGGCA GACGAAACAT TTACTGATGC AAATGGAGAA
    AAATCTGCCT CGCCTAGTGA TCTTTCCTGC CCATTTGGAT GGATTTGGGA TGATGATGGA
    TGGATGCGTG ATATAAATCG AGCTGTTGAT GAAAATGGTT GGGAATACGG CCTTACCATT
    CCTCCCGACA GTAAACCAAA GTCTTGGGTT GCAGCTGAAA AAATGTACCA CACAAACAGA
    CGTAGAAGGC TGGTGCGGAA GAGAAAGAAA GATCCCAAAG TGTCAACTAC CTCAAAGGCA
    GCGTTGACTC CGCAGGAACA AGAAGGATGG GAATATGCTG CTCTGATAGG CTGGAAGTTT
    CACATTACAC CACGCAGCTC AGACACATTC CGCCGAAGAC GCTGGAGGAG GAAAATGGCT
    CCTTCTGATC AACATGGTGC AGCAGCAATC TTTAAACTGG AGGGTGCTCT AGGAACTGAT
    ATGACTGAAG ATGAGGAGAA AAAAGGTTCT GAAAAGCAGA CCGCAACAAA TGTCTTTGGT
    GCAAACACGC CGATTGTTTC CTGTACATTT GACAAATTCT ACACCTACCA CTTGCGTTGC
    TACATTTATC AAGCCCGGGG TCTCACGCCA TTGGATAAAG ACAGCTTTTC AGACCCATAT
    GCACATGTAT CATTCCTTCA CCGAAGTAAA ACTACAGAAA CCATCCGCTC CACTCTGAAT
    CCAACATGGG ATCAGACACT TATATTTAAT ACTATTGATA TCTACGGAGA CCCCCATGCA
    GTTGCACAGA ATCCTCCAAA TGTAGTTATT GAGATATTTG ACTACGACCA AGTGGGAAAA
    GACGAGTTTC TTGGCAGAAG TGTGTGCATG CCTATGGTGA AGTTGAATCC TGAGGTTGAT
    ATTGCACCTA AACTTCTGTG GTATCCTGTC ATGAATTCAA ATAAGCATTG TGGGGACCTC
    CTTCTTGCTG CAGAGCTTAT CATTCGAGAA AAGGATGGGT CCAACTTGCC AATTCTTCCA
    TCACAGAGAG CACCACAGAT ATACATGGTG CCACAAGGAA TTCGACCAGT GGTCCAACTA
    ACGGCTATTG AGATTCTTAC TTGGGGCCTG CGGAATATGA AAAGTTACCA GCTGGCGTCT
    GTGACATCTC CGAGCCTTAT TGTTGAATGT GGTGGAGAAA TTGTTGAGAC TGCAGTGATC
    AAGAACCTTA AAAAAACGCC AAACTTTTAT AGCTCTGTCC TATTTATGAA AGTGCTTCTG
    CCAAAAGATG AAATGTACGT ACCTCCTATC ATTATCAAAA TTGTTGACCA TCGTCCTTTT
    GGAAGGAAAC CTGTTGTGGG ACAATGCACT ATTGAGTGCT TGGAAGAGTT TAGATGTGAT
    CCCTACCTCA CTAAACACGA AGATGCTCCA GAACTCAGAG TTGCACGCTT AACTTCCTCC
    CCACTTCGGG ATGTTGTGAT AGAAGTAGAA GACACAAAAC CATTACTGGC TAATCAGCTA
    CAAGAAAAGG AGGAAGAAGT AGTGGACTGG TGGAGCAAAT ACTATGCTTC AACTGGAGAA
    ACTGAGAAGT GTGGGCAGTA TATTCAGAAA GGATACACCA CCTTAAAGGT CTATAAATGT
    GAGCTGGAGA ATGTTTCTGA GTTCCGTGGC CTTACAGACT TCTGTGACAC ATTCAAGCTG
    TACAGAGGAA AAGCAGAAGA CAGTGATGAC CCTTCTGTTG TAGGAGAGTT TAAAGGTTCC
    TTCCGTATCT ACCCACTTCC AGATGATCCA AACATTCCAT ATCCTCCTCG ACAATTCCTA
    GAATTGCCAG GAACTGAATC TCAGGAGTGT ATTGTTCGTA TATACATTGT GAGAGGCATA
    GACCTGCAAC CAAAGGATAA CAATGGACTG TGTGACCCAT ATATTAAAAT TACATTGAAT
    AAAAAGGTGA TTGAAGATCG AGATCACTAC ATACCAAATA CACTGAACCC CTTATTTGGC
    AGAATGTATG AACTCAGCTG CTTCCTACCT CAAGAAAAGG ACCTCAAGAT CTCTGTGTAT
    GATTATGATA CATTAACCCG GGATGAAAAA GTCGGCGAAA CAACAATAGA TCTGGAGAAC
    AGATTCCTCT CTCGGTTTGG AAGCCATTGT GGCTTGCCAC AGACTTACTG CATCTCAGGA
    ATAAACCAAT GGAGGGACCA GCTGACACCT ACCCAAATCC TACAAAACTT CGCCAGATTA
    AAATCCTCTC CTCCCCCTGT GTTTTCAGAC AATGGAACAA GACTTACTTT TTCCAGCAAA
    GACTATACAT TAGAGGAATT TGAAAATAAT AGAAAGATTC ACCAGCATCT CGGACCACCA
    AACGAGCGTC TTGCTCTGTA TGTCCTTAGA ACTCAAGGGC TTGTTCCAGA GCATGTTGAA
    ACTAGAACTT TGTATAGCAC TTTCCAGCCA AACATTTCCC AAGGAAAACT AGAAATGTGG
    GTTGATGTTT TCCCAAAAAG TTTAGGACCT CCAGGGCCAC CTTTCAACAT TACTCCACGC
    AAAGCAAAGA AGTATGTACT TCGTGTTATT GTTTGGAATA CCAAAGATGT CATTCTAGAT
    GAGAAAAGTA TTACTGGAGA AGAAATGAGT GATATTTACG TGAAGGGGTG GATTCCTGGC
    AATGAAGAAA ACAAGCAGAA AACCGACGTA CACTACAGGT CACTGGATGG GGAAGGAAAC
    TTTAATTGGA GATTTGTTTT TCCATTTGAA TATCTTCCAG CCGAACAGTT GTGCATTGTT
    TCTAAGAAGG AACATTTCTG GAGCCTTGAC AAAACGGAAT TTAAGCTCCC ACCCAAACTA
    ATTCTTCAAA TATGGGACAA TGATAAATTC TCCTTGGATG ACTACTTAGG TTTTGTAGAA
    CTTGATTTAC ATCGAACAAC AATCCCTGCG AAAGTCCCAG AAAAATGCAG TTTTAACTTA
    CTTGATCAAG ACAAGCATTC AAAGGTGGCC TCTCTGTTTG AGCAGAAATC CATGAAGGGA
    TGGTGGCCAT GCCATGCAGA GAAGGATGGG AAAAGGATTT TAGCTGGGAA AATTGAGATG
    ACTCTTGAGG TCCTGAATGA AAAAGATGCA GAAGAAAGGC CTGCTGGCAA GGGACGAGAT
    GAGCCCAACA TGAACCCCAA GTTAGACCCA CCAAACCGAC CAGACACCTC CTTCCTCTGG
    TTTACAAACC CATGCAAGAC AATGAAATTT ATCATATGGC GCAGATTTAA ATGGGTGTTT
    ATTGGACTCA TCATCCTGCT GCTTGTACTT CTGTTCCTTG GAGTTTTCTT CTATTCTTTG
    CCGGGCTATG TTTCTATGAA GATTGTGAAA CCCAATCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61090
    Clone ID Related Accession (Same CDS sequence) XM_012965825.3
    Accession Version XM_012965825.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821279.1
    CDS152..6301
    Translation

    Target ORF information:

    RefSeq Version XM_012965825.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965825.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCCGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGGGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAGGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61091
    Clone ID Related Accession (Same CDS sequence) XM_012965826.3
    Accession Version XM_012965826.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965826.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCCGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGGGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGCTAC AAGAAAAGGA GGAAGAAGTA GTGGACTGGT GGAGCAAATA CTATGCTTCA
    ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT ATTCAGAAAG GATACACCAC CTTAAAGGTC
    TATAAATGTG AGCTGGAGAA TGTTTCTGAG TTCCGTGGCC TTACAGACTT CTGTGACACA
    TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC AGTGATGACC CTTCTGTTGT AGGAGAGTTT
    AAAGGTTCCT TCCGTATCTA CCCACTTCCA GATGATCCAA ACATTCCATA TCCTCCTCGA
    CAATTCCTAG AATTGCCAGG AACTGAATCT CAGGAGTGTA TTGTTCGTAT ATACATTGTG
    AGAGGCATAG ACCTGCAACC AAAGGATAAC AATGGACTGT GTGACCCATA TATTAAAATT
    ACATTGAATA AAAAGGTGAT TGAAGATCGA GATCACTACA TACCAAATAC ACTGAACCCC
    TTATTTGGCA GAATGTATGA ACTCAGCTGC TTCCTACCTC AAGAAAAGGA CCTCAAGATC
    TCTGTGTATG ATTATGATAC ATTAACCCGG GATGAAAAAG TCGGCGAAAC AACAATAGAT
    CTGGAGAACA GATTCCTCTC TCGGTTTGGA AGCCATTGTG GCTTGCCACA GACTTACTGC
    ATCTCAGGAA TAAACCAATG GAGGGACCAG CTGACACCTA CCCAAATCCT ACAAAACTTC
    GCCAGATTAA AATCCTCTCC TCCCCCTGTG TTTTCAGACA ATGGAACAAG ACTTACTTTT
    TCCAGCAAAG ACTATACATT AGAGGAATTT GAAAATAATA GAAAGATTCA CCAGCATCTC
    GGACCACCAA ACGAGCGTCT TGCTCTGTAT GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG
    CATGTTGAAA CTAGAACTTT GTATAGCACT TTCCAGCCAA ACATTTCCCA AGGAAAACTA
    GAAATGTGGG TTGATGTTTT CCCAAAAAGT TTAGGACCTC CAGGGCCACC TTTCAACATT
    ACTCCACGCA AAGCAAAGAA GTATGTACTT CGTGTTATTG TTTGGAATAC CAAAGATGTC
    ATTCTAGATG AGAAAAGTAT TACTGGAGAA GAAATGAGTG ATATTTACGT GAAGGGGTGG
    ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA ACCGACGTAC ACTACAGGTC ACTGGATGGG
    GAAGGAAACT TTAATTGGAG ATTTGTTTTT CCATTTGAAT ATCTTCCAGC CGAACAGTTG
    TGCATTGTTT CTAAGAAGGA ACATTTCTGG AGCCTTGACA AAACGGAATT TAAGCTCCCA
    CCCAAACTAA TTCTTCAAAT ATGGGACAAT GATAAATTCT CCTTGGATGA CTACTTAGGT
    TTTGTAGAAC TTGATTTACA TCGAACAACA ATCCCTGCGA AAGTCCCAGA AAAATGCAGT
    TTTAACTTAC TTGATCAAGA CAAGCATTCA AAGGTGGCCT CTCTGTTTGA GCAGAAATCC
    ATGAAGGGAT GGTGGCCATG CCATGCAGAG AAGGATGGGA AAAGGATTTT AGCTGGGAAA
    ATTGAGATGA CTCTTGAGGT CCTGAATGAA AAAGATGCAG AAGAAAGGCC TGCTGGCAAG
    GGACGAGATG AGCCCAACAT GAACCCCAAG TTAGACCCAC CAAACCGACC AGACACCTCC
    TTCCTCTGGT TTACAAACCC ATGCAAGACA ATGAAATTTA TCATATGGCG CAGATTTAAA
    TGGGTGTTTA TTGGACTCAT CATCCTGCTG CTTGTACTTC TGTTCCTTGG AGTTTTCTTC
    TATTCTTTGC CGGGCTATGT TTCTATGAAG ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821280.1
    CDS152..6262
    Translation

    Target ORF information:

    RefSeq Version XM_012965826.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin, gene 1 (myof.1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965826.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCCGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGGGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGCTAC AAGAAAAGGA GGAAGAAGTA GTGGACTGGT GGAGCAAATA CTATGCTTCA
    ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT ATTCAGAAAG GATACACCAC CTTAAAGGTC
    TATAAATGTG AGCTGGAGAA TGTTTCTGAG TTCCGTGGCC TTACAGACTT CTGTGACACA
    TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC AGTGATGACC CTTCTGTTGT AGGAGAGTTT
    AAAGGTTCCT TCCGTATCTA CCCACTTCCA GATGATCCAA ACATTCCATA TCCTCCTCGA
    CAATTCCTAG AATTGCCAGG AACTGAATCT CAGGAGTGTA TTGTTCGTAT ATACATTGTG
    AGAGGCATAG ACCTGCAACC AAAGGATAAC AATGGACTGT GTGACCCATA TATTAAAATT
    ACATTGAATA AAAAGGTGAT TGAAGATCGA GATCACTACA TACCAAATAC ACTGAACCCC
    TTATTTGGCA GAATGTATGA ACTCAGCTGC TTCCTACCTC AAGAAAAGGA CCTCAAGATC
    TCTGTGTATG ATTATGATAC ATTAACCCGG GATGAAAAAG TCGGCGAAAC AACAATAGAT
    CTGGAGAACA GATTCCTCTC TCGGTTTGGA AGCCATTGTG GCTTGCCACA GACTTACTGC
    ATCTCAGGAA TAAACCAATG GAGGGACCAG CTGACACCTA CCCAAATCCT ACAAAACTTC
    GCCAGATTAA AATCCTCTCC TCCCCCTGTG TTTTCAGACA ATGGAACAAG ACTTACTTTT
    TCCAGCAAAG ACTATACATT AGAGGAATTT GAAAATAATA GAAAGATTCA CCAGCATCTC
    GGACCACCAA ACGAGCGTCT TGCTCTGTAT GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG
    CATGTTGAAA CTAGAACTTT GTATAGCACT TTCCAGCCAA ACATTTCCCA AGGAAAACTA
    GAAATGTGGG TTGATGTTTT CCCAAAAAGT TTAGGACCTC CAGGGCCACC TTTCAACATT
    ACTCCACGCA AAGCAAAGAA GTATGTACTT CGTGTTATTG TTTGGAATAC CAAAGATGTC
    ATTCTAGATG AGAAAAGTAT TACTGGAGAA GAAATGAGTG ATATTTACGT GAAGGGGTGG
    ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA ACCGACGTAC ACTACAGGTC ACTGGATGGG
    GAAGGAAACT TTAATTGGAG ATTTGTTTTT CCATTTGAAT ATCTTCCAGC CGAACAGTTG
    TGCATTGTTT CTAAGAAGGA ACATTTCTGG AGCCTTGACA AAACGGAATT TAAGCTCCCA
    CCCAAACTAA TTCTTCAAAT ATGGGACAAT GATAAATTCT CCTTGGATGA CTACTTAGGT
    TTTGTAGAAC TTGATTTACA TCGAACAACA ATCCCTGCGA AAGTCCCAGA AAAATGCAGT
    TTTAACTTAC TTGATCAAGA CAAGCATTCA AAGGTGGCCT CTCTGTTTGA GCAGAAATCC
    ATGAAGGGAT GGTGGCCATG CCATGCAGAG AAGGATGGGA AAAGGATTTT AGCTGGGAAA
    ATTGAGATGA CTCTTGAGGT CCTGAATGAA AAAGATGCAG AAGAAAGGCC TGCTGGCAAG
    GGACGAGATG AGCCCAACAT GAACCCCAAG TTAGACCCAC CAAACCGACC AGACACCTCC
    TTCCTCTGGT TTACAAACCC ATGCAAGACA ATGAAATTTA TCATATGGCG CAGATTTAAA
    TGGGTGTTTA TTGGACTCAT CATCCTGCTG CTTGTACTTC TGTTCCTTGG AGTTTTCTTC
    TATTCTTTGC CGGGCTATGT TTCTATGAAG ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29938
    Clone ID Related Accession (Same CDS sequence) XM_012965820.2
    Accession Version XM_012965820.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGTTG TTAAGCAGTA TGTCAACAGC AATCGGAAAA
    ATGGCTTCTC CAACTACAGT GCATCTACAA GAAAAGGAGG AAGAAGTAGT GGACTGGTGG
    AGCAAATACT ATGCTTCAAC TGGAGAAACT GAGAAGTGTG GGCAGTATAT TCAGAAAGGA
    TACACCACCT TAAAGGTCTA TAAATGTGAG CTGGAGAATG TTTCTGAGTT CCGTGGCCTT
    ACAGACTTCT GTGACACATT CAAGCTGTAC AGAGGAAAAG CAGAAGACAG TGATGACCCT
    TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC CGTATCTACC CACTTCCAGA TGATCCAAAC
    ATTCCATATC CTCCTCGACA ATTCCTAGAA TTGCCAGGAA CTGAATCTCA GGAGTGTATT
    GTTCGTATAT ACATTGTGAG AGGCATAGAC CTGCAACCAA AGGATAACAA TGGACTGTGT
    GACCCATATA TTAAAATTAC ATTGAATAAA AAGGTGATTG AAGATCGAGA TCACTACATA
    CCAAATACAC TGAACCCCTT ATTTGGCAGA ATGTATGAAC TCAGCTGCTT CCTACCTCAA
    GAAAAGGACC TCAAGATCTC TGTGTATGAT TATGATACAT TAACCCGGGA TGAAAAAGTC
    GGCGAAACAA CAATAGATCT GGAGAACAGA TTCCTCTCTC GGTTTGGAAG CCATTGTGGC
    TTGCCACAGA CTTACTGCAT CTCAGGAATA AACCAATGGA GGGACCAGCT GACACCTACC
    CAAATCCTAC AAAACTTCGC CAGATTAAAA TCCTCTCCTC CCCCTGTGTT TTCAGACAAT
    GGAACAAGAC TTACTTTTTC CAGCAAAGAC TATACATTAG AGGAATTTGA AAATAATAGA
    AAGATTCACC AGCATCTCGG ACCACCAAAC GAGCGTCTTG CTCTGTATGT CCTTAGAACT
    CAAGGGCTTG TTCCAGAGCA TGTTGAAACT AGAACTTTGT ATAGCACTTT CCAGCCAAAC
    ATTTCCCAAG GAAAACTAGA AATGTGGGTT GATGTTTTCC CAAAAAGTTT AGGACCTCCA
    GGGCCACCTT TCAACATTAC TCCACGCAAA GCAAAGAAGT ATGTACTTCG TGTTATTGTT
    TGGAATACCA AAGATGTCAT TCTAGATGAG AAAAGTATTA CTGGAGAAGA AATGAGTGAT
    ATTTACGTGA AGGGGTGGAT TCCTGGCAAT GAAGAAAACA AGCAGAAAAC CGACGTACAC
    TACAGGTCAC TGGATGGGGA AGGAAACTTT AATTGGAGAT TTGTTTTTCC ATTTGAATAT
    CTTCCAGCCG AACAGTTGTG CATTGTTTCT AAGAAGGAAC ATTTCTGGAG CCTTGACAAA
    ACGGAATTTA AGCTCCCACC CAAACTAATT CTTCAAATAT GGGACAATGA TAAATTCTCC
    TTGGATGACT ACTTAGGTTT TGTAGAACTT GATTTACATC GAACAACAAT CCCTGCGAAA
    GTCCCAGAAA AATGCAGTTT TAACTTACTT GATCAAGACA AGCATTCAAA GGTGGCCTCT
    CTGTTTGAGC AGAAATCCAT GAAGGGATGG TGGCCATGCC ATGCAGAGAA GGATGGGAAA
    AGGATTTTAG CTGGGAAAAT TGAGATGACT CTTGAAGTCC TGAATGAAAA AGATGCAGAA
    GAAAGGCCTG CTGGCAAGGG ACGAGATGAG CCCAACATGA ACCCCAAGTT AGACCCACCA
    AACCGACCAG ACACCTCCTT CCTCTGGTTT ACAAACCCAT GCAAGACAAT GAAATTTATC
    ATATGGCGCA GATTTAAATG GGTGTTTATT GGACTCATCA TCCTGCTGCT TGTACTTCTG
    TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG GGCTATGTTT CTATGAAGAT TGTGAAACCC
    AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821274.1
    CDS147..6395
    Translation

    Target ORF information:

    RefSeq Version XM_012965820.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965820.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGTTG TTAAGCAGTA TGTCAACAGC AATCGGAAAA
    ATGGCTTCTC CAACTACAGT GCATCTACAA GAAAAGGAGG AAGAAGTAGT GGACTGGTGG
    AGCAAATACT ATGCTTCAAC TGGAGAAACT GAGAAGTGTG GGCAGTATAT TCAGAAAGGA
    TACACCACCT TAAAGGTCTA TAAATGTGAG CTGGAGAATG TTTCTGAGTT CCGTGGCCTT
    ACAGACTTCT GTGACACATT CAAGCTGTAC AGAGGAAAAG CAGAAGACAG TGATGACCCT
    TCTGTTGTAG GAGAGTTTAA AGGTTCCTTC CGTATCTACC CACTTCCAGA TGATCCAAAC
    ATTCCATATC CTCCTCGACA ATTCCTAGAA TTGCCAGGAA CTGAATCTCA GGAGTGTATT
    GTTCGTATAT ACATTGTGAG AGGCATAGAC CTGCAACCAA AGGATAACAA TGGACTGTGT
    GACCCATATA TTAAAATTAC ATTGAATAAA AAGGTGATTG AAGATCGAGA TCACTACATA
    CCAAATACAC TGAACCCCTT ATTTGGCAGA ATGTATGAAC TCAGCTGCTT CCTACCTCAA
    GAAAAGGACC TCAAGATCTC TGTGTATGAT TATGATACAT TAACCCGGGA TGAAAAAGTC
    GGCGAAACAA CAATAGATCT GGAGAACAGA TTCCTCTCTC GGTTTGGAAG CCATTGTGGC
    TTGCCACAGA CTTACTGCAT CTCAGGAATA AACCAATGGA GGGACCAGCT GACACCTACC
    CAAATCCTAC AAAACTTCGC CAGATTAAAA TCCTCTCCTC CCCCTGTGTT TTCAGACAAT
    GGAACAAGAC TTACTTTTTC CAGCAAAGAC TATACATTAG AGGAATTTGA AAATAATAGA
    AAGATTCACC AGCATCTCGG ACCACCAAAC GAGCGTCTTG CTCTGTATGT CCTTAGAACT
    CAAGGGCTTG TTCCAGAGCA TGTTGAAACT AGAACTTTGT ATAGCACTTT CCAGCCAAAC
    ATTTCCCAAG GAAAACTAGA AATGTGGGTT GATGTTTTCC CAAAAAGTTT AGGACCTCCA
    GGGCCACCTT TCAACATTAC TCCACGCAAA GCAAAGAAGT ATGTACTTCG TGTTATTGTT
    TGGAATACCA AAGATGTCAT TCTAGATGAG AAAAGTATTA CTGGAGAAGA AATGAGTGAT
    ATTTACGTGA AGGGGTGGAT TCCTGGCAAT GAAGAAAACA AGCAGAAAAC CGACGTACAC
    TACAGGTCAC TGGATGGGGA AGGAAACTTT AATTGGAGAT TTGTTTTTCC ATTTGAATAT
    CTTCCAGCCG AACAGTTGTG CATTGTTTCT AAGAAGGAAC ATTTCTGGAG CCTTGACAAA
    ACGGAATTTA AGCTCCCACC CAAACTAATT CTTCAAATAT GGGACAATGA TAAATTCTCC
    TTGGATGACT ACTTAGGTTT TGTAGAACTT GATTTACATC GAACAACAAT CCCTGCGAAA
    GTCCCAGAAA AATGCAGTTT TAACTTACTT GATCAAGACA AGCATTCAAA GGTGGCCTCT
    CTGTTTGAGC AGAAATCCAT GAAGGGATGG TGGCCATGCC ATGCAGAGAA GGATGGGAAA
    AGGATTTTAG CTGGGAAAAT TGAGATGACT CTTGAAGTCC TGAATGAAAA AGATGCAGAA
    GAAAGGCCTG CTGGCAAGGG ACGAGATGAG CCCAACATGA ACCCCAAGTT AGACCCACCA
    AACCGACCAG ACACCTCCTT CCTCTGGTTT ACAAACCCAT GCAAGACAAT GAAATTTATC
    ATATGGCGCA GATTTAAATG GGTGTTTATT GGACTCATCA TCCTGCTGCT TGTACTTCTG
    TTCCTTGGAG TTTTCTTCTA TTCTTTGCCG GGCTATGTTT CTATGAAGAT TGTGAAACCC
    AATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29939
    Clone ID Related Accession (Same CDS sequence) XM_012965821.2
    Accession Version XM_012965821.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCGGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGTGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGGGAT CTTCTGGCAT TGGGATATTA GGCGGGAAAA ATCTGTCGTT GTTAAGCAGT
    ATGTCAACAG CAATCGGAAA AATGGCTTCT CCAACTACAG TGCATCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAAGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821275.1
    CDS147..6356
    Translation

    Target ORF information:

    RefSeq Version XM_012965821.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965821.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCGGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGTGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGGGAT CTTCTGGCAT TGGGATATTA GGCGGGAAAA ATCTGTCGTT GTTAAGCAGT
    ATGTCAACAG CAATCGGAAA AATGGCTTCT CCAACTACAG TGCATCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAAGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29940
    Clone ID Related Accession (Same CDS sequence) XM_012965822.2
    Accession Version XM_012965822.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGTTGTT AAGCAGTATG
    TCAACAGCAA TCGGAAAAAT GGCTTCTCCA ACTACAGTGC ATCTACAAGA AAAGGAGGAA
    GAAGTAGTGG ACTGGTGGAG CAAATACTAT GCTTCAACTG GAGAAACTGA GAAGTGTGGG
    CAGTATATTC AGAAAGGATA CACCACCTTA AAGGTCTATA AATGTGAGCT GGAGAATGTT
    TCTGAGTTCC GTGGCCTTAC AGACTTCTGT GACACATTCA AGCTGTACAG AGGAAAAGCA
    GAAGACAGTG ATGACCCTTC TGTTGTAGGA GAGTTTAAAG GTTCCTTCCG TATCTACCCA
    CTTCCAGATG ATCCAAACAT TCCATATCCT CCTCGACAAT TCCTAGAATT GCCAGGAACT
    GAATCTCAGG AGTGTATTGT TCGTATATAC ATTGTGAGAG GCATAGACCT GCAACCAAAG
    GATAACAATG GACTGTGTGA CCCATATATT AAAATTACAT TGAATAAAAA GGTGATTGAA
    GATCGAGATC ACTACATACC AAATACACTG AACCCCTTAT TTGGCAGAAT GTATGAACTC
    AGCTGCTTCC TACCTCAAGA AAAGGACCTC AAGATCTCTG TGTATGATTA TGATACATTA
    ACCCGGGATG AAAAAGTCGG CGAAACAACA ATAGATCTGG AGAACAGATT CCTCTCTCGG
    TTTGGAAGCC ATTGTGGCTT GCCACAGACT TACTGCATCT CAGGAATAAA CCAATGGAGG
    GACCAGCTGA CACCTACCCA AATCCTACAA AACTTCGCCA GATTAAAATC CTCTCCTCCC
    CCTGTGTTTT CAGACAATGG AACAAGACTT ACTTTTTCCA GCAAAGACTA TACATTAGAG
    GAATTTGAAA ATAATAGAAA GATTCACCAG CATCTCGGAC CACCAAACGA GCGTCTTGCT
    CTGTATGTCC TTAGAACTCA AGGGCTTGTT CCAGAGCATG TTGAAACTAG AACTTTGTAT
    AGCACTTTCC AGCCAAACAT TTCCCAAGGA AAACTAGAAA TGTGGGTTGA TGTTTTCCCA
    AAAAGTTTAG GACCTCCAGG GCCACCTTTC AACATTACTC CACGCAAAGC AAAGAAGTAT
    GTACTTCGTG TTATTGTTTG GAATACCAAA GATGTCATTC TAGATGAGAA AAGTATTACT
    GGAGAAGAAA TGAGTGATAT TTACGTGAAG GGGTGGATTC CTGGCAATGA AGAAAACAAG
    CAGAAAACCG ACGTACACTA CAGGTCACTG GATGGGGAAG GAAACTTTAA TTGGAGATTT
    GTTTTTCCAT TTGAATATCT TCCAGCCGAA CAGTTGTGCA TTGTTTCTAA GAAGGAACAT
    TTCTGGAGCC TTGACAAAAC GGAATTTAAG CTCCCACCCA AACTAATTCT TCAAATATGG
    GACAATGATA AATTCTCCTT GGATGACTAC TTAGGTTTTG TAGAACTTGA TTTACATCGA
    ACAACAATCC CTGCGAAAGT CCCAGAAAAA TGCAGTTTTA ACTTACTTGA TCAAGACAAG
    CATTCAAAGG TGGCCTCTCT GTTTGAGCAG AAATCCATGA AGGGATGGTG GCCATGCCAT
    GCAGAGAAGG ATGGGAAAAG GATTTTAGCT GGGAAAATTG AGATGACTCT TGAAGTCCTG
    AATGAAAAAG ATGCAGAAGA AAGGCCTGCT GGCAAGGGAC GAGATGAGCC CAACATGAAC
    CCCAAGTTAG ACCCACCAAA CCGACCAGAC ACCTCCTTCC TCTGGTTTAC AAACCCATGC
    AAGACAATGA AATTTATCAT ATGGCGCAGA TTTAAATGGG TGTTTATTGG ACTCATCATC
    CTGCTGCTTG TACTTCTGTT CCTTGGAGTT TTCTTCTATT CTTTGCCGGG CTATGTTTCT
    ATGAAGATTG TGAAACCCAA TCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821276.1
    CDS147..6353
    Translation

    Target ORF information:

    RefSeq Version XM_012965822.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965822.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGTTGTT AAGCAGTATG
    TCAACAGCAA TCGGAAAAAT GGCTTCTCCA ACTACAGTGC ATCTACAAGA AAAGGAGGAA
    GAAGTAGTGG ACTGGTGGAG CAAATACTAT GCTTCAACTG GAGAAACTGA GAAGTGTGGG
    CAGTATATTC AGAAAGGATA CACCACCTTA AAGGTCTATA AATGTGAGCT GGAGAATGTT
    TCTGAGTTCC GTGGCCTTAC AGACTTCTGT GACACATTCA AGCTGTACAG AGGAAAAGCA
    GAAGACAGTG ATGACCCTTC TGTTGTAGGA GAGTTTAAAG GTTCCTTCCG TATCTACCCA
    CTTCCAGATG ATCCAAACAT TCCATATCCT CCTCGACAAT TCCTAGAATT GCCAGGAACT
    GAATCTCAGG AGTGTATTGT TCGTATATAC ATTGTGAGAG GCATAGACCT GCAACCAAAG
    GATAACAATG GACTGTGTGA CCCATATATT AAAATTACAT TGAATAAAAA GGTGATTGAA
    GATCGAGATC ACTACATACC AAATACACTG AACCCCTTAT TTGGCAGAAT GTATGAACTC
    AGCTGCTTCC TACCTCAAGA AAAGGACCTC AAGATCTCTG TGTATGATTA TGATACATTA
    ACCCGGGATG AAAAAGTCGG CGAAACAACA ATAGATCTGG AGAACAGATT CCTCTCTCGG
    TTTGGAAGCC ATTGTGGCTT GCCACAGACT TACTGCATCT CAGGAATAAA CCAATGGAGG
    GACCAGCTGA CACCTACCCA AATCCTACAA AACTTCGCCA GATTAAAATC CTCTCCTCCC
    CCTGTGTTTT CAGACAATGG AACAAGACTT ACTTTTTCCA GCAAAGACTA TACATTAGAG
    GAATTTGAAA ATAATAGAAA GATTCACCAG CATCTCGGAC CACCAAACGA GCGTCTTGCT
    CTGTATGTCC TTAGAACTCA AGGGCTTGTT CCAGAGCATG TTGAAACTAG AACTTTGTAT
    AGCACTTTCC AGCCAAACAT TTCCCAAGGA AAACTAGAAA TGTGGGTTGA TGTTTTCCCA
    AAAAGTTTAG GACCTCCAGG GCCACCTTTC AACATTACTC CACGCAAAGC AAAGAAGTAT
    GTACTTCGTG TTATTGTTTG GAATACCAAA GATGTCATTC TAGATGAGAA AAGTATTACT
    GGAGAAGAAA TGAGTGATAT TTACGTGAAG GGGTGGATTC CTGGCAATGA AGAAAACAAG
    CAGAAAACCG ACGTACACTA CAGGTCACTG GATGGGGAAG GAAACTTTAA TTGGAGATTT
    GTTTTTCCAT TTGAATATCT TCCAGCCGAA CAGTTGTGCA TTGTTTCTAA GAAGGAACAT
    TTCTGGAGCC TTGACAAAAC GGAATTTAAG CTCCCACCCA AACTAATTCT TCAAATATGG
    GACAATGATA AATTCTCCTT GGATGACTAC TTAGGTTTTG TAGAACTTGA TTTACATCGA
    ACAACAATCC CTGCGAAAGT CCCAGAAAAA TGCAGTTTTA ACTTACTTGA TCAAGACAAG
    CATTCAAAGG TGGCCTCTCT GTTTGAGCAG AAATCCATGA AGGGATGGTG GCCATGCCAT
    GCAGAGAAGG ATGGGAAAAG GATTTTAGCT GGGAAAATTG AGATGACTCT TGAAGTCCTG
    AATGAAAAAG ATGCAGAAGA AAGGCCTGCT GGCAAGGGAC GAGATGAGCC CAACATGAAC
    CCCAAGTTAG ACCCACCAAA CCGACCAGAC ACCTCCTTCC TCTGGTTTAC AAACCCATGC
    AAGACAATGA AATTTATCAT ATGGCGCAGA TTTAAATGGG TGTTTATTGG ACTCATCATC
    CTGCTGCTTG TACTTCTGTT CCTTGGAGTT TTCTTCTATT CTTTGCCGGG CTATGTTTCT
    ATGAAGATTG TGAAACCCAA TCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29941
    Clone ID Related Accession (Same CDS sequence) XM_012965823.2
    Accession Version XM_012965823.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGCTA CAAGAAAAGG AGGAAGAAGT AGTGGACTGG
    TGGAGCAAAT ACTATGCTTC AACTGGAGAA ACTGAGAAGT GTGGGCAGTA TATTCAGAAA
    GGATACACCA CCTTAAAGGT CTATAAATGT GAGCTGGAGA ATGTTTCTGA GTTCCGTGGC
    CTTACAGACT TCTGTGACAC ATTCAAGCTG TACAGAGGAA AAGCAGAAGA CAGTGATGAC
    CCTTCTGTTG TAGGAGAGTT TAAAGGTTCC TTCCGTATCT ACCCACTTCC AGATGATCCA
    AACATTCCAT ATCCTCCTCG ACAATTCCTA GAATTGCCAG GAACTGAATC TCAGGAGTGT
    ATTGTTCGTA TATACATTGT GAGAGGCATA GACCTGCAAC CAAAGGATAA CAATGGACTG
    TGTGACCCAT ATATTAAAAT TACATTGAAT AAAAAGGTGA TTGAAGATCG AGATCACTAC
    ATACCAAATA CACTGAACCC CTTATTTGGC AGAATGTATG AACTCAGCTG CTTCCTACCT
    CAAGAAAAGG ACCTCAAGAT CTCTGTGTAT GATTATGATA CATTAACCCG GGATGAAAAA
    GTCGGCGAAA CAACAATAGA TCTGGAGAAC AGATTCCTCT CTCGGTTTGG AAGCCATTGT
    GGCTTGCCAC AGACTTACTG CATCTCAGGA ATAAACCAAT GGAGGGACCA GCTGACACCT
    ACCCAAATCC TACAAAACTT CGCCAGATTA AAATCCTCTC CTCCCCCTGT GTTTTCAGAC
    AATGGAACAA GACTTACTTT TTCCAGCAAA GACTATACAT TAGAGGAATT TGAAAATAAT
    AGAAAGATTC ACCAGCATCT CGGACCACCA AACGAGCGTC TTGCTCTGTA TGTCCTTAGA
    ACTCAAGGGC TTGTTCCAGA GCATGTTGAA ACTAGAACTT TGTATAGCAC TTTCCAGCCA
    AACATTTCCC AAGGAAAACT AGAAATGTGG GTTGATGTTT TCCCAAAAAG TTTAGGACCT
    CCAGGGCCAC CTTTCAACAT TACTCCACGC AAAGCAAAGA AGTATGTACT TCGTGTTATT
    GTTTGGAATA CCAAAGATGT CATTCTAGAT GAGAAAAGTA TTACTGGAGA AGAAATGAGT
    GATATTTACG TGAAGGGGTG GATTCCTGGC AATGAAGAAA ACAAGCAGAA AACCGACGTA
    CACTACAGGT CACTGGATGG GGAAGGAAAC TTTAATTGGA GATTTGTTTT TCCATTTGAA
    TATCTTCCAG CCGAACAGTT GTGCATTGTT TCTAAGAAGG AACATTTCTG GAGCCTTGAC
    AAAACGGAAT TTAAGCTCCC ACCCAAACTA ATTCTTCAAA TATGGGACAA TGATAAATTC
    TCCTTGGATG ACTACTTAGG TTTTGTAGAA CTTGATTTAC ATCGAACAAC AATCCCTGCG
    AAAGTCCCAG AAAAATGCAG TTTTAACTTA CTTGATCAAG ACAAGCATTC AAAGGTGGCC
    TCTCTGTTTG AGCAGAAATC CATGAAGGGA TGGTGGCCAT GCCATGCAGA GAAGGATGGG
    AAAAGGATTT TAGCTGGGAA AATTGAGATG ACTCTTGAAG TCCTGAATGA AAAAGATGCA
    GAAGAAAGGC CTGCTGGCAA GGGACGAGAT GAGCCCAACA TGAACCCCAA GTTAGACCCA
    CCAAACCGAC CAGACACCTC CTTCCTCTGG TTTACAAACC CATGCAAGAC AATGAAATTT
    ATCATATGGC GCAGATTTAA ATGGGTGTTT ATTGGACTCA TCATCCTGCT GCTTGTACTT
    CTGTTCCTTG GAGTTTTCTT CTATTCTTTG CCGGGCTATG TTTCTATGAA GATTGTGAAA
    CCCAATCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821277.1
    CDS147..6338
    Translation

    Target ORF information:

    RefSeq Version XM_012965823.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGGGATC TTCTGGCATT
    GGGATATTAG GCGGGAAAAA TCTGTCGCTA CAAGAAAAGG AGGAAGAAGT AGTGGACTGG
    TGGAGCAAAT ACTATGCTTC AACTGGAGAA ACTGAGAAGT GTGGGCAGTA TATTCAGAAA
    GGATACACCA CCTTAAAGGT CTATAAATGT GAGCTGGAGA ATGTTTCTGA GTTCCGTGGC
    CTTACAGACT TCTGTGACAC ATTCAAGCTG TACAGAGGAA AAGCAGAAGA CAGTGATGAC
    CCTTCTGTTG TAGGAGAGTT TAAAGGTTCC TTCCGTATCT ACCCACTTCC AGATGATCCA
    AACATTCCAT ATCCTCCTCG ACAATTCCTA GAATTGCCAG GAACTGAATC TCAGGAGTGT
    ATTGTTCGTA TATACATTGT GAGAGGCATA GACCTGCAAC CAAAGGATAA CAATGGACTG
    TGTGACCCAT ATATTAAAAT TACATTGAAT AAAAAGGTGA TTGAAGATCG AGATCACTAC
    ATACCAAATA CACTGAACCC CTTATTTGGC AGAATGTATG AACTCAGCTG CTTCCTACCT
    CAAGAAAAGG ACCTCAAGAT CTCTGTGTAT GATTATGATA CATTAACCCG GGATGAAAAA
    GTCGGCGAAA CAACAATAGA TCTGGAGAAC AGATTCCTCT CTCGGTTTGG AAGCCATTGT
    GGCTTGCCAC AGACTTACTG CATCTCAGGA ATAAACCAAT GGAGGGACCA GCTGACACCT
    ACCCAAATCC TACAAAACTT CGCCAGATTA AAATCCTCTC CTCCCCCTGT GTTTTCAGAC
    AATGGAACAA GACTTACTTT TTCCAGCAAA GACTATACAT TAGAGGAATT TGAAAATAAT
    AGAAAGATTC ACCAGCATCT CGGACCACCA AACGAGCGTC TTGCTCTGTA TGTCCTTAGA
    ACTCAAGGGC TTGTTCCAGA GCATGTTGAA ACTAGAACTT TGTATAGCAC TTTCCAGCCA
    AACATTTCCC AAGGAAAACT AGAAATGTGG GTTGATGTTT TCCCAAAAAG TTTAGGACCT
    CCAGGGCCAC CTTTCAACAT TACTCCACGC AAAGCAAAGA AGTATGTACT TCGTGTTATT
    GTTTGGAATA CCAAAGATGT CATTCTAGAT GAGAAAAGTA TTACTGGAGA AGAAATGAGT
    GATATTTACG TGAAGGGGTG GATTCCTGGC AATGAAGAAA ACAAGCAGAA AACCGACGTA
    CACTACAGGT CACTGGATGG GGAAGGAAAC TTTAATTGGA GATTTGTTTT TCCATTTGAA
    TATCTTCCAG CCGAACAGTT GTGCATTGTT TCTAAGAAGG AACATTTCTG GAGCCTTGAC
    AAAACGGAAT TTAAGCTCCC ACCCAAACTA ATTCTTCAAA TATGGGACAA TGATAAATTC
    TCCTTGGATG ACTACTTAGG TTTTGTAGAA CTTGATTTAC ATCGAACAAC AATCCCTGCG
    AAAGTCCCAG AAAAATGCAG TTTTAACTTA CTTGATCAAG ACAAGCATTC AAAGGTGGCC
    TCTCTGTTTG AGCAGAAATC CATGAAGGGA TGGTGGCCAT GCCATGCAGA GAAGGATGGG
    AAAAGGATTT TAGCTGGGAA AATTGAGATG ACTCTTGAAG TCCTGAATGA AAAAGATGCA
    GAAGAAAGGC CTGCTGGCAA GGGACGAGAT GAGCCCAACA TGAACCCCAA GTTAGACCCA
    CCAAACCGAC CAGACACCTC CTTCCTCTGG TTTACAAACC CATGCAAGAC AATGAAATTT
    ATCATATGGC GCAGATTTAA ATGGGTGTTT ATTGGACTCA TCATCCTGCT GCTTGTACTT
    CTGTTCCTTG GAGTTTTCTT CTATTCTTTG CCGGGCTATG TTTCTATGAA GATTGTGAAA
    CCCAATCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29942
    Clone ID Related Accession (Same CDS sequence) XM_012965825.2
    Accession Version XM_012965825.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAAGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821279.1
    CDS147..6296
    Translation

    Target ORF information:

    RefSeq Version XM_012965825.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGAATATG ACTCCACAGG AACTGGGTCT
    TCATCACTGG AAGCAACCAC AGAAAAGGAA GTAGGGTTTC TGCCAACATT TGGACCTTGC
    TACTTGAATC TTTATGGAAG CCCAAGAGAA TATACAGGGT TCCCAGATCC CTATGATGAC
    CTGAATTTTG GGAAGGGGGA AGGTGTTGCT TATAGAGGAC GTGTTTTGGT GGAACTCACT
    ACTAAACTGG ATAACTCCTC AATTAAAAAG ATTGAAGACA TTTCTAGTGA TGACATCCTT
    GTTGTTGAGA AATATCAGCG CAGACGAAAA TACTGTTTAT GTGCAGTTTT CCATTCAGCC
    ACCATGATAC AAGACATTGG TGAAGCTATA CAGTTTGAAG TCAGCATTGG CAATTATGGC
    AACAAATTTG ATAGCACTTG TAAACCACTG GCATCTACAA CCCAATACAG CCGTCCCATA
    TTTGATGGCA ACTATTATTA CTACCTACCC TGGTCATTCA CAAAGCCTGT TGTAACATTA
    ACTTCTTACT GGGAAGACAT AAGCCATCGA CTGGACATAG TTAATATTCT TATAGCCATG
    ACAGATCGAC TGCAATCCAA TATATCCACA CTGAAATCTG CAATACAGGC AAAGCTTCCT
    GATGTACGTC TTGCAGAAAT ATGGATGAGG CTTATTGATC AACTTATTGA AGATACGATG
    AGGCCAATGC CATCCCTGGA AGGAAAAGCC AATGTTACAG TGCTGGATAA GCAGCGCGAT
    AAACTTCGGC AGACCTCCCT CAAATACATA CAGGAGGCAG CTATTAAAAT GAGGGGGGAA
    GCAACAGATG TGAAAGCAAC ACTGACGGAG ATTGAGGACT GGTTGGATCG ATTACAGCAG
    CTGTCTGAAG AGCCACAGAA CAGCATGCCC GACGTGATCA TCTGGATGAT TCGAGCAGAG
    AAGAGACTTG CCTACGCACG TGTCCCAGCT CATCAGGTTC TCTTTTCCAA AACCAGCGAG
    GAAGCCTGTG GAAAATACTG CGGAAAGACC CAAACTGTTT TTTTACAGTA CCCACTTGAC
    AAGACCAAAG GTTTAAAAAT TCCTACTGAG CTACGAGTGA ACATCTGGCT GGGACTGTCG
    GAAGTTGAAA AGAAGTTTAA CAGCTATTCA GAAGGAACCT TTAGTGTTTA TGCTGAAATG
    TATGAAAATC AAGCTCTCTT ACTTGGGAAA TGGGGCACCA CAGGTCTTCT CAAGCGCCAC
    AAATTTTCTG ATGTCACTGG CTCAATAAAG CTAAAGAGAG AAAGCTTTTT GCCCCCAAAG
    GGATGGGAAT GGGAAGATGA CTGGAAAGTG GACCCTGAAA GGAGCTTACT AACTGAAGCC
    GATGCTGGTC ATACTGAGTT TACAGATGAA ATCTTTGAAA ATGAGGCTCG CTATCCAGGT
    GGTGAATGGA AGAAGGCAGA CGAAACATTT ACTGATGCAA ATGGAGAAAA ATCTGCCTCG
    CCTAGTGATC TTTCCTGCCC ATTTGGATGG ATTTGGGATG ATGATGGATG GATGCGTGAT
    ATAAATCGAG CTGTTGATGA AAATGGTTGG GAATACGGCC TTACCATTCC TCCGGACAGT
    AAACCAAAGT CTTGGGTTGC AGCTGAAAAA ATGTACCACA CAAACAGACG TAGAAGGCTG
    GTGCGGAAGA GAAAGAAAGA TCCCAAAGTG TCAACTACCT CAAAGGCAGC GTTGACTCCG
    CAGGAACAAG AAGGATGGGA ATATGCTGCT CTGATAGGCT GGAAGTTTCA CATTACACCA
    CGCAGCTCAG ACACATTCCG CCGAAGACGC TGGAGGAGGA AAATGGCTCC TTCTGATCAA
    CATGGTGCAG CAGCAATCTT TAAACTGGAG GGTGCTCTAG GAACTGATAT GACTGAAGAT
    GAGGAGAAAA AAGGTTCTGA AAAGCAGACC GCAACAAATG TCTTTGGTGC AAACACGCCG
    ATTGTTTCCT GTACATTTGA CAAATTCTAC ACCTACCACT TGCGTTGCTA CATTTATCAA
    GCCCGGGGTC TCACGCCATT GGATAAAGAC AGCTTTTCAG ACCCATATGC ACATGTATCA
    TTCCTTCACC GAAGTAAAAC TACAGAAACC ATCCGCTCCA CTCTGAATCC AACATGGGAT
    CAGACACTTA TATTTAATAC TATTGATATC TACGGAGACC CCCATGCAGT TGCACAGAAT
    CCTCCAAATG TAGTTATTGA GATATTTGAC TACGACCAAG TGGGAAAAGA CGAGTTTCTT
    GGCAGAAGTG TGTGCATGCC TATGGTGAAG TTGAATCCTG AGGTTGATAT TGCACCTAAA
    CTTCTGTGGT ATCCTGTCAT GAATTCAAAT AAGCATTGTG GGGACCTCCT TCTTGCTGCA
    GAGCTTATCA TTCGAGAAAA GGATGGGTCC AACTTGCCAA TTCTTCCATC ACAGAGAGCA
    CCACAGATAT ACATGGTGCC ACAAGGAATT CGACCAGTGG TCCAACTAAC GGCTATTGAG
    ATTCTTACTT GGGGCCTGCG GAATATGAAA AGTTACCAGC TGGCGTCTGT GACATCTCCG
    AGCCTTATTG TTGAATGTGG TGGAGAAATT GTTGAGACTG CAGTGATCAA GAACCTTAAA
    AAAACGCCAA ACTTTTATAG CTCTGTCCTA TTTATGAAAG TGCTTCTGCC AAAAGATGAA
    ATGTACGTAC CTCCTATCAT TATCAAAATT GTTGACCATC GTCCTTTTGG AAGGAAACCT
    GTTGTGGGAC AATGCACTAT TGAGTGCTTG GAAGAGTTTA GATGTGATCC CTACCTCACT
    AAACACGAAG ATGCTCCAGA ACTCAGAGTT GCACGCTTAA CTTCCTCCCC ACTTCGTGAT
    GTTGTGATAG AAGTAGAAGA CACAAAACCA TTACTGGCTA ATCAGCTACA AGAAAAGGAG
    GAAGAAGTAG TGGACTGGTG GAGCAAATAC TATGCTTCAA CTGGAGAAAC TGAGAAGTGT
    GGGCAGTATA TTCAGAAAGG ATACACCACC TTAAAGGTCT ATAAATGTGA GCTGGAGAAT
    GTTTCTGAGT TCCGTGGCCT TACAGACTTC TGTGACACAT TCAAGCTGTA CAGAGGAAAA
    GCAGAAGACA GTGATGACCC TTCTGTTGTA GGAGAGTTTA AAGGTTCCTT CCGTATCTAC
    CCACTTCCAG ATGATCCAAA CATTCCATAT CCTCCTCGAC AATTCCTAGA ATTGCCAGGA
    ACTGAATCTC AGGAGTGTAT TGTTCGTATA TACATTGTGA GAGGCATAGA CCTGCAACCA
    AAGGATAACA ATGGACTGTG TGACCCATAT ATTAAAATTA CATTGAATAA AAAGGTGATT
    GAAGATCGAG ATCACTACAT ACCAAATACA CTGAACCCCT TATTTGGCAG AATGTATGAA
    CTCAGCTGCT TCCTACCTCA AGAAAAGGAC CTCAAGATCT CTGTGTATGA TTATGATACA
    TTAACCCGGG ATGAAAAAGT CGGCGAAACA ACAATAGATC TGGAGAACAG ATTCCTCTCT
    CGGTTTGGAA GCCATTGTGG CTTGCCACAG ACTTACTGCA TCTCAGGAAT AAACCAATGG
    AGGGACCAGC TGACACCTAC CCAAATCCTA CAAAACTTCG CCAGATTAAA ATCCTCTCCT
    CCCCCTGTGT TTTCAGACAA TGGAACAAGA CTTACTTTTT CCAGCAAAGA CTATACATTA
    GAGGAATTTG AAAATAATAG AAAGATTCAC CAGCATCTCG GACCACCAAA CGAGCGTCTT
    GCTCTGTATG TCCTTAGAAC TCAAGGGCTT GTTCCAGAGC ATGTTGAAAC TAGAACTTTG
    TATAGCACTT TCCAGCCAAA CATTTCCCAA GGAAAACTAG AAATGTGGGT TGATGTTTTC
    CCAAAAAGTT TAGGACCTCC AGGGCCACCT TTCAACATTA CTCCACGCAA AGCAAAGAAG
    TATGTACTTC GTGTTATTGT TTGGAATACC AAAGATGTCA TTCTAGATGA GAAAAGTATT
    ACTGGAGAAG AAATGAGTGA TATTTACGTG AAGGGGTGGA TTCCTGGCAA TGAAGAAAAC
    AAGCAGAAAA CCGACGTACA CTACAGGTCA CTGGATGGGG AAGGAAACTT TAATTGGAGA
    TTTGTTTTTC CATTTGAATA TCTTCCAGCC GAACAGTTGT GCATTGTTTC TAAGAAGGAA
    CATTTCTGGA GCCTTGACAA AACGGAATTT AAGCTCCCAC CCAAACTAAT TCTTCAAATA
    TGGGACAATG ATAAATTCTC CTTGGATGAC TACTTAGGTT TTGTAGAACT TGATTTACAT
    CGAACAACAA TCCCTGCGAA AGTCCCAGAA AAATGCAGTT TTAACTTACT TGATCAAGAC
    AAGCATTCAA AGGTGGCCTC TCTGTTTGAG CAGAAATCCA TGAAGGGATG GTGGCCATGC
    CATGCAGAGA AGGATGGGAA AAGGATTTTA GCTGGGAAAA TTGAGATGAC TCTTGAAGTC
    CTGAATGAAA AAGATGCAGA AGAAAGGCCT GCTGGCAAGG GACGAGATGA GCCCAACATG
    AACCCCAAGT TAGACCCACC AAACCGACCA GACACCTCCT TCCTCTGGTT TACAAACCCA
    TGCAAGACAA TGAAATTTAT CATATGGCGC AGATTTAAAT GGGTGTTTAT TGGACTCATC
    ATCCTGCTGC TTGTACTTCT GTTCCTTGGA GTTTTCTTCT ATTCTTTGCC GGGCTATGTT
    TCTATGAAGA TTGTGAAACC CAATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29943
    Clone ID Related Accession (Same CDS sequence) XM_012965826.2
    Accession Version XM_012965826.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCGGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGTGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGCTAC AAGAAAAGGA GGAAGAAGTA GTGGACTGGT GGAGCAAATA CTATGCTTCA
    ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT ATTCAGAAAG GATACACCAC CTTAAAGGTC
    TATAAATGTG AGCTGGAGAA TGTTTCTGAG TTCCGTGGCC TTACAGACTT CTGTGACACA
    TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC AGTGATGACC CTTCTGTTGT AGGAGAGTTT
    AAAGGTTCCT TCCGTATCTA CCCACTTCCA GATGATCCAA ACATTCCATA TCCTCCTCGA
    CAATTCCTAG AATTGCCAGG AACTGAATCT CAGGAGTGTA TTGTTCGTAT ATACATTGTG
    AGAGGCATAG ACCTGCAACC AAAGGATAAC AATGGACTGT GTGACCCATA TATTAAAATT
    ACATTGAATA AAAAGGTGAT TGAAGATCGA GATCACTACA TACCAAATAC ACTGAACCCC
    TTATTTGGCA GAATGTATGA ACTCAGCTGC TTCCTACCTC AAGAAAAGGA CCTCAAGATC
    TCTGTGTATG ATTATGATAC ATTAACCCGG GATGAAAAAG TCGGCGAAAC AACAATAGAT
    CTGGAGAACA GATTCCTCTC TCGGTTTGGA AGCCATTGTG GCTTGCCACA GACTTACTGC
    ATCTCAGGAA TAAACCAATG GAGGGACCAG CTGACACCTA CCCAAATCCT ACAAAACTTC
    GCCAGATTAA AATCCTCTCC TCCCCCTGTG TTTTCAGACA ATGGAACAAG ACTTACTTTT
    TCCAGCAAAG ACTATACATT AGAGGAATTT GAAAATAATA GAAAGATTCA CCAGCATCTC
    GGACCACCAA ACGAGCGTCT TGCTCTGTAT GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG
    CATGTTGAAA CTAGAACTTT GTATAGCACT TTCCAGCCAA ACATTTCCCA AGGAAAACTA
    GAAATGTGGG TTGATGTTTT CCCAAAAAGT TTAGGACCTC CAGGGCCACC TTTCAACATT
    ACTCCACGCA AAGCAAAGAA GTATGTACTT CGTGTTATTG TTTGGAATAC CAAAGATGTC
    ATTCTAGATG AGAAAAGTAT TACTGGAGAA GAAATGAGTG ATATTTACGT GAAGGGGTGG
    ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA ACCGACGTAC ACTACAGGTC ACTGGATGGG
    GAAGGAAACT TTAATTGGAG ATTTGTTTTT CCATTTGAAT ATCTTCCAGC CGAACAGTTG
    TGCATTGTTT CTAAGAAGGA ACATTTCTGG AGCCTTGACA AAACGGAATT TAAGCTCCCA
    CCCAAACTAA TTCTTCAAAT ATGGGACAAT GATAAATTCT CCTTGGATGA CTACTTAGGT
    TTTGTAGAAC TTGATTTACA TCGAACAACA ATCCCTGCGA AAGTCCCAGA AAAATGCAGT
    TTTAACTTAC TTGATCAAGA CAAGCATTCA AAGGTGGCCT CTCTGTTTGA GCAGAAATCC
    ATGAAGGGAT GGTGGCCATG CCATGCAGAG AAGGATGGGA AAAGGATTTT AGCTGGGAAA
    ATTGAGATGA CTCTTGAAGT CCTGAATGAA AAAGATGCAG AAGAAAGGCC TGCTGGCAAG
    GGACGAGATG AGCCCAACAT GAACCCCAAG TTAGACCCAC CAAACCGACC AGACACCTCC
    TTCCTCTGGT TTACAAACCC ATGCAAGACA ATGAAATTTA TCATATGGCG CAGATTTAAA
    TGGGTGTTTA TTGGACTCAT CATCCTGCTG CTTGTACTTC TGTTCCTTGG AGTTTTCTTC
    TATTCTTTGC CGGGCTATGT TTCTATGAAG ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821280.1
    CDS147..6257
    Translation

    Target ORF information:

    RefSeq Version XM_012965826.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myoferlin (myof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965826.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTAAAAG TCATTGTGGA ATCTGCAGCT AACATCCCTA AAACCAAGCT TGGGAAGCCA 
    GACCCTATAG TAACTATCAT CTTTAAAGGG GAGAAGAAGA AAACAAAGCC TGTGGACAAT
    GAATTAAATC CAATATGGAA TGAGCTGCTG GAATTTGATT TAAAAGGAAT ACCCCTGGAC
    TACAGCGCAT CGCTTGAAGT TATTGTCAAG GATTTCGAAA CTATCGGAAA AGACAAGTTT
    ATTGGAACCA TAGTTGTATC ACTAAAAGAA TTCTCTGCTG GCCAAGTTCG ATCACTTCCA
    TTCAAAGGTT TAGCTTTACA GAGTGAAAAA GGACAACCCA TTGGTTCAAC CATTGATTTA
    ATGATTTCCT ATGAACCACC CCCATCTGCT ATTAGTAACC CCACAGACCC AGGAGGAACT
    ACAATTATTC AAGGAGATGG AGAAAATGAT GAAGAAGAAG ACAGAGATAT TGTGGATGCT
    GGGTTCAACC CCAGTGTTCC TGGAGCTCCT GGACAAACTG ACACCCAGAT CGCTCGGAGA
    CTTGTTAAAG GAAAGAAAAC AAGAAGAATC CTTTCAAATA AACCTCAAGA CTTTCAGATA
    CGCATTCGGG TTATTGAAGG ACGTCAGCTA CCTGGTAACA ATATCAAACC TGTTGTTAAA
    GTTAGTGTGG GAGGTCAGAC ACACCGCACA AGAATCAAGA GAGGAAATAA CCCCTATTTT
    GATGAGATCT TCTTTTATAA TGTCAACATG ACACCTCTGG AACTGCTTGA TGAATCTGTA
    ATATTTCGGT TGTTCAATTC TGGTTCAATA CGAGCTGACA GTTTAATTGG AGAGTTTAAG
    CTTGATGTTG GATACATATA TGATGAACCA GGCCATGCAG TTATGCGAAA ATGGGTTCTT
    CTCAATGATC CTGATGACTC TAGTTCCGGT GCAAAGGGAT ATCTGAAAGT CAGCATGTTT
    GTAGTTGGGA CTGGAGATGA GCCGCCTGTG GAAAAACGAG ACAGGGAAAT GGAAGATGAT
    GATGTTGAAA GTAATCTGTT ATTGCCTGCC GGAGTGGCAC TGCGATGGGT GACGTTCTTT
    CTTAAAATTT ACAGAGCTGA AGATATTCCC CAAATGGATG ATGCTTTTGC ACAGACTGTC
    AAAGAGATAT TTGGAGCAGA TTCAGACAAG AAAAATTTGG TTGATCCATT CGTGGAAGTG
    TCTTTTGCTG GGAAAAAGGT GTGCACAAAC AGAATTGAGA AAAATGCCAA TCCTGAGTGG
    AATCAAGCTG TGAATCTCCA AATAAAGTTT CCTTCAATGT GCGAGAATAT AAAGTTAACC
    GTTTATGATT GGGATAGACT TACTAAAAAT GATGCGGTAG GAACAACATG TCTAAGTCTG
    TCGAAGATTG CAGCTTCTGG TGGTGAAATA GAAGCAACCA CAGAAAAGGA AGTAGGGTTT
    CTGCCAACAT TTGGACCTTG CTACTTGAAT CTTTATGGAA GCCCAAGAGA ATATACAGGG
    TTCCCAGATC CCTATGATGA CCTGAATTTT GGGAAGGGGG AAGGTGTTGC TTATAGAGGA
    CGTGTTTTGG TGGAACTCAC TACTAAACTG GATAACTCCT CAATTAAAAA GATTGAAGAC
    ATTTCTAGTG ATGACATCCT TGTTGTTGAG AAATATCAGC GCAGACGAAA ATACTGTTTA
    TGTGCAGTTT TCCATTCAGC CACCATGATA CAAGACATTG GTGAAGCTAT ACAGTTTGAA
    GTCAGCATTG GCAATTATGG CAACAAATTT GATAGCACTT GTAAACCACT GGCATCTACA
    ACCCAATACA GCCGTCCCAT ATTTGATGGC AACTATTATT ACTACCTACC CTGGTCATTC
    ACAAAGCCTG TTGTAACATT AACTTCTTAC TGGGAAGACA TAAGCCATCG ACTGGACATA
    GTTAATATTC TTATAGCCAT GACAGATCGA CTGCAATCCA ATATATCCAC ACTGAAATCT
    GCAATACAGG CAAAGCTTCC TGATGTACGT CTTGCAGAAA TATGGATGAG GCTTATTGAT
    CAACTTATTG AAGATACGAT GAGGCCAATG CCATCCCTGG AAGGAAAAGC CAATGTTACA
    GTGCTGGATA AGCAGCGCGA TAAACTTCGG CAGACCTCCC TCAAATACAT ACAGGAGGCA
    GCTATTAAAA TGAGGGGGGA AGCAACAGAT GTGAAAGCAA CACTGACGGA GATTGAGGAC
    TGGTTGGATC GATTACAGCA GCTGTCTGAA GAGCCACAGA ACAGCATGCC CGACGTGATC
    ATCTGGATGA TTCGAGCAGA GAAGAGACTT GCCTACGCAC GTGTCCCAGC TCATCAGGTT
    CTCTTTTCCA AAACCAGCGA GGAAGCCTGT GGAAAATACT GCGGAAAGAC CCAAACTGTT
    TTTTTACAGT ACCCACTTGA CAAGACCAAA GGTTTAAAAA TTCCTACTGA GCTACGAGTG
    AACATCTGGC TGGGACTGTC GGAAGTTGAA AAGAAGTTTA ACAGCTATTC AGAAGGAACC
    TTTAGTGTTT ATGCTGAAAT GTATGAAAAT CAAGCTCTCT TACTTGGGAA ATGGGGCACC
    ACAGGTCTTC TCAAGCGCCA CAAATTTTCT GATGTCACTG GCTCAATAAA GCTAAAGAGA
    GAAAGCTTTT TGCCCCCAAA GGGATGGGAA TGGGAAGATG ACTGGAAAGT GGACCCTGAA
    AGGAGCTTAC TAACTGAAGC CGATGCTGGT CATACTGAGT TTACAGATGA AATCTTTGAA
    AATGAGGCTC GCTATCCAGG TGGTGAATGG AAGAAGGCAG ACGAAACATT TACTGATGCA
    AATGGAGAAA AATCTGCCTC GCCTAGTGAT CTTTCCTGCC CATTTGGATG GATTTGGGAT
    GATGATGGAT GGATGCGTGA TATAAATCGA GCTGTTGATG AAAATGGTTG GGAATACGGC
    CTTACCATTC CTCCGGACAG TAAACCAAAG TCTTGGGTTG CAGCTGAAAA AATGTACCAC
    ACAAACAGAC GTAGAAGGCT GGTGCGGAAG AGAAAGAAAG ATCCCAAAGT GTCAACTACC
    TCAAAGGCAG CGTTGACTCC GCAGGAACAA GAAGGATGGG AATATGCTGC TCTGATAGGC
    TGGAAGTTTC ACATTACACC ACGCAGCTCA GACACATTCC GCCGAAGACG CTGGAGGAGG
    AAAATGGCTC CTTCTGATCA ACATGGTGCA GCAGCAATCT TTAAACTGGA GGGTGCTCTA
    GGAACTGATA TGACTGAAGA TGAGGAGAAA AAAGGTTCTG AAAAGCAGAC CGCAACAAAT
    GTCTTTGGTG CAAACACGCC GATTGTTTCC TGTACATTTG ACAAATTCTA CACCTACCAC
    TTGCGTTGCT ACATTTATCA AGCCCGGGGT CTCACGCCAT TGGATAAAGA CAGCTTTTCA
    GACCCATATG CACATGTATC ATTCCTTCAC CGAAGTAAAA CTACAGAAAC CATCCGCTCC
    ACTCTGAATC CAACATGGGA TCAGACACTT ATATTTAATA CTATTGATAT CTACGGAGAC
    CCCCATGCAG TTGCACAGAA TCCTCCAAAT GTAGTTATTG AGATATTTGA CTACGACCAA
    GTGGGAAAAG ACGAGTTTCT TGGCAGAAGT GTGTGCATGC CTATGGTGAA GTTGAATCCT
    GAGGTTGATA TTGCACCTAA ACTTCTGTGG TATCCTGTCA TGAATTCAAA TAAGCATTGT
    GGGGACCTCC TTCTTGCTGC AGAGCTTATC ATTCGAGAAA AGGATGGGTC CAACTTGCCA
    ATTCTTCCAT CACAGAGAGC ACCACAGATA TACATGGTGC CACAAGGAAT TCGACCAGTG
    GTCCAACTAA CGGCTATTGA GATTCTTACT TGGGGCCTGC GGAATATGAA AAGTTACCAG
    CTGGCGTCTG TGACATCTCC GAGCCTTATT GTTGAATGTG GTGGAGAAAT TGTTGAGACT
    GCAGTGATCA AGAACCTTAA AAAAACGCCA AACTTTTATA GCTCTGTCCT ATTTATGAAA
    GTGCTTCTGC CAAAAGATGA AATGTACGTA CCTCCTATCA TTATCAAAAT TGTTGACCAT
    CGTCCTTTTG GAAGGAAACC TGTTGTGGGA CAATGCACTA TTGAGTGCTT GGAAGAGTTT
    AGATGTGATC CCTACCTCAC TAAACACGAA GATGCTCCAG AACTCAGAGT TGCACGCTTA
    ACTTCCTCCC CACTTCGTGA TGTTGTGATA GAAGTAGAAG ACACAAAACC ATTACTGGCT
    AATCAGCTAC AAGAAAAGGA GGAAGAAGTA GTGGACTGGT GGAGCAAATA CTATGCTTCA
    ACTGGAGAAA CTGAGAAGTG TGGGCAGTAT ATTCAGAAAG GATACACCAC CTTAAAGGTC
    TATAAATGTG AGCTGGAGAA TGTTTCTGAG TTCCGTGGCC TTACAGACTT CTGTGACACA
    TTCAAGCTGT ACAGAGGAAA AGCAGAAGAC AGTGATGACC CTTCTGTTGT AGGAGAGTTT
    AAAGGTTCCT TCCGTATCTA CCCACTTCCA GATGATCCAA ACATTCCATA TCCTCCTCGA
    CAATTCCTAG AATTGCCAGG AACTGAATCT CAGGAGTGTA TTGTTCGTAT ATACATTGTG
    AGAGGCATAG ACCTGCAACC AAAGGATAAC AATGGACTGT GTGACCCATA TATTAAAATT
    ACATTGAATA AAAAGGTGAT TGAAGATCGA GATCACTACA TACCAAATAC ACTGAACCCC
    TTATTTGGCA GAATGTATGA ACTCAGCTGC TTCCTACCTC AAGAAAAGGA CCTCAAGATC
    TCTGTGTATG ATTATGATAC ATTAACCCGG GATGAAAAAG TCGGCGAAAC AACAATAGAT
    CTGGAGAACA GATTCCTCTC TCGGTTTGGA AGCCATTGTG GCTTGCCACA GACTTACTGC
    ATCTCAGGAA TAAACCAATG GAGGGACCAG CTGACACCTA CCCAAATCCT ACAAAACTTC
    GCCAGATTAA AATCCTCTCC TCCCCCTGTG TTTTCAGACA ATGGAACAAG ACTTACTTTT
    TCCAGCAAAG ACTATACATT AGAGGAATTT GAAAATAATA GAAAGATTCA CCAGCATCTC
    GGACCACCAA ACGAGCGTCT TGCTCTGTAT GTCCTTAGAA CTCAAGGGCT TGTTCCAGAG
    CATGTTGAAA CTAGAACTTT GTATAGCACT TTCCAGCCAA ACATTTCCCA AGGAAAACTA
    GAAATGTGGG TTGATGTTTT CCCAAAAAGT TTAGGACCTC CAGGGCCACC TTTCAACATT
    ACTCCACGCA AAGCAAAGAA GTATGTACTT CGTGTTATTG TTTGGAATAC CAAAGATGTC
    ATTCTAGATG AGAAAAGTAT TACTGGAGAA GAAATGAGTG ATATTTACGT GAAGGGGTGG
    ATTCCTGGCA ATGAAGAAAA CAAGCAGAAA ACCGACGTAC ACTACAGGTC ACTGGATGGG
    GAAGGAAACT TTAATTGGAG ATTTGTTTTT CCATTTGAAT ATCTTCCAGC CGAACAGTTG
    TGCATTGTTT CTAAGAAGGA ACATTTCTGG AGCCTTGACA AAACGGAATT TAAGCTCCCA
    CCCAAACTAA TTCTTCAAAT ATGGGACAAT GATAAATTCT CCTTGGATGA CTACTTAGGT
    TTTGTAGAAC TTGATTTACA TCGAACAACA ATCCCTGCGA AAGTCCCAGA AAAATGCAGT
    TTTAACTTAC TTGATCAAGA CAAGCATTCA AAGGTGGCCT CTCTGTTTGA GCAGAAATCC
    ATGAAGGGAT GGTGGCCATG CCATGCAGAG AAGGATGGGA AAAGGATTTT AGCTGGGAAA
    ATTGAGATGA CTCTTGAAGT CCTGAATGAA AAAGATGCAG AAGAAAGGCC TGCTGGCAAG
    GGACGAGATG AGCCCAACAT GAACCCCAAG TTAGACCCAC CAAACCGACC AGACACCTCC
    TTCCTCTGGT TTACAAACCC ATGCAAGACA ATGAAATTTA TCATATGGCG CAGATTTAAA
    TGGGTGTTTA TTGGACTCAT CATCCTGCTG CTTGTACTTC TGTTCCTTGG AGTTTTCTTC
    TATTCTTTGC CGGGCTATGT TTCTATGAAG ATTGTGAAAC CCAATCTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.