MYOF gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_133337.2	NP_579899.1	myoferlin isoform b
XM_005269693.4	XP_005269750.1	myoferlin isoform X1
XM_011539632.2	XP_011537934.1	myoferlin isoform X3
XM_017016068.2	XP_016871557.1	myoferlin isoform X4
XM_017016068.1	XP_016871557.1	myoferlin isoform X4
NM_133337.3	NP_579899.1	myoferlin isoform b
XM_005269694.4	XP_005269751.1	myoferlin isoform X2
XM_017016069.1	XP_016871558.1	myoferlin isoform X4
NM_013451.4	NP_038479.1	myoferlin isoform a
NM_013451.3	NP_038479.1	myoferlin isoform a
XM_017016070.1	XP_016871559.1	myoferlin isoform X5
XM_005269694.5	XP_005269751.1	myoferlin isoform X2
XM_017016070.2	XP_016871559.1	myoferlin isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu91296	XM_017016070.2 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu44204	XM_017016069.1 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44204	XM_017016068.2 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu28602	NM_013451.4 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28607	NM_133337.3 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu44202	XM_005269693.4 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44203	XM_005269694.5 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu66943	XM_011539632.2 Latest version!	Homo sapiens myoferlin (MYOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu44204	XM_017016068.1	Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu28607	NM_133337.2	Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28602	NM_013451.3	Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu44203	XM_005269694.4	Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu91296	XM_017016070.1	Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

MYOF ( XM_017016070.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871559.1 Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu91296
Clone ID Related Accession (Same CDS sequence)	XM_017016070.1 , XM_017016070.2
Accession Version	XM_017016070.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGCC ATATGAGGAG 
TTTCAAGAAA TGACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871559.1
CDS	311..5671
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQPYEEFQEMTE

Target ORF information:

RefSeq Version	XM_017016070.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016070.1

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGCC ATATGAGGAG 
TTTCAAGAAA TGACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_017016070.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871559.1 Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.

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CloneID	OHu91296
Clone ID Related Accession (Same CDS sequence)	XM_017016070.1 , XM_017016070.2
Accession Version	XM_017016070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGCC ATATGAGGAG 
TTTCAAGAAA TGACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871559.1
CDS	312..5672
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQPYEEFQEMTE

Target ORF information:

RefSeq Version	XM_017016070.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016070.2

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGCC ATATGAGGAG 
TTTCAAGAAA TGACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_017016068.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871557.1 Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.

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CloneID	OHu44204
Clone ID Related Accession (Same CDS sequence)	XM_017016068.2 , XM_017016068.1 , XM_017016069.1
Accession Version	XM_017016068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871557.1
CDS	309..6125
Translation	MGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_017016068.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016068.2

ORF Insert Sequence:

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_017016068.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871557.1 Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.

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CloneID	OHu44204
Clone ID Related Accession (Same CDS sequence)	XM_017016068.2 , XM_017016068.1 , XM_017016069.1
Accession Version	XM_017016068.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871557.1
CDS	310..6126
Translation	MGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_017016068.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016068.1

ORF Insert Sequence:

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_017016069.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871558.1 Homo sapiens myoferlin (MYOF), transcript variant X5, mRNA.

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CloneID	OHu44204
Clone ID Related Accession (Same CDS sequence)	XM_017016068.2 , XM_017016068.1 , XM_017016069.1
Accession Version	XM_017016069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871558.1
CDS	355..6171
Translation	MGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_017016069.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016069.1

ORF Insert Sequence:

ATGGGAGGAG ATGGGGAAGA AGATGAAGGT GATGAAGACA GGTTGGACAA TGCAGTCAGG 
GGCCCTGGGC CCAAGGGGCC AGTTGGGACG GTGTCGGAAG CTCAGCTTGC TCGGAGGCTC 
ACCAAAGTAA AGAACAGCCG GCGGATGCTG TCAAATAAGC CACAGGACTT CCAGATCCGC 
GTCCGAGTGA TTGAGGGCCG ACAGTTAAGT GGTAACAACA TAAGGCCTGT GGTCAAAGTT 
CACGTCTGTG GCCAGACACA CCGAACAAGA ATCAAGAGAG GAAACAACCC TTTTTTTGAT 
GAGTTGTTTT TCTACAATGT CAACATGACC CCTTCTGAAT TGATGGATGA GATCATCAGC 
ATCCGGGTTT ATAATTCTCA CTCTCTGCGG GCAGATTGTC TGATGGGGGA ATTTAAGATT 
GATGTTGGAT TTGTTTATGA TGAACCTGGC CATGCTGTCA TGAGAAAGTG GCTTCTTCTC 
AATGACCCGG AAGATACCAG TTCAGGTTCT AAAGGTTATA TGAAAGTCAG CATGTTTGTC 
CTGGGAACCG GAGATGAGCC TCCTCCTGAG AGACGAGATC GTGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT CCCTGCTGGC ATTGCCCTCC GGTGGGTGAC CTTCTTGCTG 
AAAATCTACC GAGCTGAGGA CATCCCCCAG ATGGATGATG CCTTCTCACA GACAGTAAAG 
GAAATATTTG GAGGCAATGC AGATAAGAAA AATCTCGTGG ATCCTTTTGT AGAAGTTTCC 
TTTGCTGGAA AAAAGGTTTG TACAAACATA ATTGAGAAAA ATGCAAACCC AGAGTGGAAT 
CAGGTCGTCA ATCTTCAGAT CAAGTTTCCT TCAGTGTGTG AAAAAATAAA ACTAACAATA 
TATGACTGGG ACCGTCTTAC TAAAAATGAT GTAGTTGGAA CAACATATCT ACACCTCTCT 
AAAATTGCTG CCTCTGGTGG GGAAGTGGAA GATTTCTCAT CTTCGGGAAC TGGGGCTGCA 
TCATATACAG TAAACACAGG AGAAACAGAG GTAGGCTTTG TTCCAACGTT TGGACCTTGT 
TACCTGAATC TTTATGGAAG CCCCAGAGAG TACACGGGAT TCCCAGACCC CTATGATGAG 
CTGAATACTG GAAAGGGGGA AGGAGTTGCC TACAGAGGCA GGATCTTGGT TGAATTAGCC 
ACTTTTCTTG AGAAGACACC ACCAGATAAA AAGCTTGAGC CCATTTCAAA TGATGACCTG 
CTGGTTGTTG AGAAATACCA GCGAAGGCGG AAGTACAGCC TGTCTGCCGT GTTTCATTCA 
GCCACCATGT TGCAAGATGT TGGTGAGGCC ATTCAGTTTG AAGTCAGCAT TGGGAACTAT 
GGCAACAAGT TTGACACCAC CTGTAAGCCT TTGGCATCAA CAACTCAGTA CAGCCGTGCT 
GTATTTGATG GCAACTACTA TTATTACTTG CCTTGGGCCC ACACCAAGCC AGTTGTTACC 
CTGACTTCAT ACTGGGAGGA TATTAGTCAT CGCCTGGATG CGGTGAACAC TCTCCTAGCT 
ATGGCAGAAC GGCTGCAAAC AAATATAGAA GCTCTAAAAT CAGGGATACA AGGTAAAATT 
CCTGCAAACC AGCTGGCTGA ATTGTGGCTG AAGCTGATAG ATGAAGTTAT AGAAGACACG 
AGATACACGT TGCCTCTCAC AGAAGGAAAA GCCAACGTCA CAGTTCTCGA TACTCAGATC 
CGAAAGCTGC GGTCCAGGTC TCTCTCCCAA ATACATGAGG CGGCTGTGAG GATGAGGTCG 
GAAGCCACAG ATGTGAAGTC CACACTGGCA GAAATTGAGG ACTGGCTTGA TAAATTAATG 
CAGCTGACTG AAGAGCCACA GAACAGCATG CCTGACATCA TCATCTGGAT GATCCGGGGA 
GAGAAGAGAC TGGCCTATGC ACGAATTCCC GCACATCAGG TCTTGTACTC CACCAGTGGT 
GAGAATGCAT CTGGAAAATA CTGTGGGAAA ACCCAAACCA TCTTTCTGAA GTATCCACAG 
GAGAAAAACA ACGGGCCAAA GGTGCCTGTG GAGTTGCGAG TGAACATCTG GCTAGGCTTA 
AGTGCTGTGG AGAAGAAGTT TAACAGCTTC GCAGAAGGAA CTTTCACCGT CTTTGCTGAA 
ATGTATGAAA ATCAAGCTCT CATGTTTGGA AAATGGGGTA CTTCTGGATT AGTAGGACGT 
CATAAGTTTT CTGATGTCAC AGGAAAAATA AAACTCAAGA GGGAATTTTT TCTGCCTCCA 
AAAGGCTGGG AATGGGAAGG AGAGTGGATA GTTGATCCTG AAAGAAGCTT GCTGACTGAG 
GCAGATGCAG GTCACACGGA GTTCACTGAT GAAGTCTATC AGAACGAGAG CCGCTACCCC 
GGGGGCGACT GGAAGCCGGC CGAGGACACC TACACGGATG CGAACGGCGA TAAAGCAGCA 
TCACCCAGCG AGTTGACTTG TCCTCCAGGT TGGGAATGGG AAGATGATGC ATGGTCTTAT 
GACATAAATC GAGCGGTGGA TGAGAAAGGC TGGGAATATG GAATCACCAT TCCTCCTGAT 
CATAAGCCCA AATCCTGGGT TGCAGCAGAG AAAATGTACC ACACTCATAG ACGGCGAAGG 
CTGGTCCGAA AACGCAAGAA AGATTTAACA CAGACTGCTT CAAGCACCGC AAGGGCCATG 
GAGGAATTGC AAGACCAAGA GGGCTGGGAA TATGCTTCTC TAATTGGCTG GAAATTTCAC 
TGGAAACAAC GTAGTTCAGA TACCTTCCGC CGCAGACGCT GGAGGAGAAA AATGGCTCCT 
TCAGAAACAC ATGGTGCAGC TGCCATCTTT AAACTTGAAG GTGCCCTTGG GGCAGACACT 
ACCGAAGATG GGGATGAGAA GAGCCTGGAG AAACAGAAGC ACAGTGCCAC CACTGTGTTC 
GGAGCAAACA CCCCCATTGT TTCCTGCAAT TTTGACAGAG TCTACATCTA CCATCTGCGC 
TGCTATGTCT ATCAAGCCAG AAACCTCTTG GCTTTAGATA AGGATAGCTT TTCAGATCCA 
TATGCTCATA TCTGTTTCCT CCATCGGAGC AAAACCACTG AGATCATCCA TTCAACCCTG 
AATCCCACGT GGGACCAAAC AATTATATTC GATGAAGTTG AAATCTATGG GGAACCCCAA 
ACAGTTCTAC AGAATCCACC CAAAGTTATC ATGGAACTTT TTGACAATGA CCAAGTGGGC 
AAAGATGAAT TTTTAGGACG AAGCATTTTC TCTCCTGTGG TGAAACTGAA CTCAGAAATG 
GACATCACAC CCAAACTTCT CTGGCACCCA GTAATGAATG GAGACAAAGC CTGCGGGGAT 
GTTCTTGTAA CTGCAGAGCT GATTCTGAGG GGCAAGGATG GCTCCAACCT TCCCATTCTT 
CCCCCTCAAA GGGCGCCAAA TCTATACATG GTCCCCCAGG GGATCAGGCC TGTGGTCCAG 
CTCACTGCCA TTGAGATTCT AGCTTGGGGC TTAAGAAATA TGAAAAACTT CCAGATGGCT 
TCTATCACAT CCCCCAGTCT TGTTGTGGAG TGTGGAGGAG AAAGGGTGGA ATCGGTGGTG 
ATCAAAAACC TTAAGAAGAC ACCCAACTTT CCAAGTTCTG TTCTCTTCAT GAAAGTGTTC 
TTGCCCAAGG AGGAATTGTA CATGCCCCCA CTGGTGATCA AGGTCATCGA CCACAGGCAG 
TTTGGGCGGA AGCCTGTCGT CGGCCAGTGC ACCATCGAGC GCCTGGACCG CTTTCGCTGT 
GACCCTTATG CAGGGAAAGA GGACATCGTC CCACAGCTCA AAGCCTCCCT TCTGTCTGCC 
CCACCATGCC GGGACATCGT TATCGAAATG GAAGACACCA AACCATTACT GGCTTCTAAG 
TGCTTAAGCA GTATGTCAAC AGCACTCAGC AAAATGGCTT CTCCAGCGAC AGTGCATCTG 
ACAGAAAAGG AGGAAGAAAT CGTGGACTGG TGGAGTAAAT TTTATGCTTC CTCAGGGGAA 
CATGAAAAAT GCGGACAGTA TATTCAGAAA GGCTATTCCA AGCTCAAGAT ATATAATTGT 
GAACTAGAAA ATGTAGCAGA ATTTGAGGGC CTGACAGACT TCTCAGATAC GTTCAAGTTG 
TACCGAGGCA AGTCGGATGA AAATGAAGAT CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC 
TTTCGGATCT ACCCTCTGCC GGATGACCCC AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG 
GAATTACCTG ACAGCGTCCC ACAGGAATGC ACGGTTAGGA TTTACATTGT TCGAGGCTTA 
GAGCTCCAGC CCCAGGACAA CAATGGCCTG TGTGACCCTT ACATAAAAAT AACACTGGGC 
AAAAAAGTCA TTGAAGACCG AGATCACTAC ATTCCCAACA CTCTCAACCC AGTCTTTGGC 
AGGATGTACG AACTGAGCTG CTACTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CCTTTACCCG GGATGAAAAA GTAGGAGAAA CAATTATTGA TCTGGAAAAC 
CGATTCCTTT CCCGCTTTGG GTCCCACTGC GGCATACCAG AGGAGTACTG TGTTTCTGGA 
GTCAATACCT GGCGAGATCA ACTGAGACCA ACACAGCTGC TTCAAAATGT CGCCAGATTC 
AAAGGCTTCC CACAACCCAT CCTTTCCGAA GATGGGAGTA GAATCAGATA TGGAGGACGA 
GACTACAGCT TGGATGAATT TGAAGCCAAC AAAATCCTGC ACCAGCACCT CGGGGCCCCT 
GAAGAGCGGC TTGCTCTTCA CATCCTCAGG ACTCAGGGGC TGGTCCCTGA GCACGTGGAA 
ACAAGGACTT TGCACAGCAC CTTCCAGCCC AACATTTCCC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAGAG TTTGGGGCCA CCAGGCCCTC CTTTCAACAT CACACCCCGG 
AAAGCCAAGA AATACTACCT GCGTGTGATC ATCTGGAACA CCAAGGACGT TATCTTGGAC 
GAGAAAAGCA TCACAGGAGA GGAAATGAGT GACATCTACG TCAAAGGCTG GATTCCTGGC 
AATGAAGAAA ACAAACAGAA AACAGATGTC CATTACAGAT CTTTGGATGG TGAAGGGAAT 
TTTAACTGGC GATTTGTTTT CCCGTTTGAC TACCTTCCAG CCGAACAACT CTGTATCGTT 
GCGAAAAAAG AGCATTTCTG GAGTATTGAC CAAACGGAAT TTCGAATCCC ACCCAGGCTG 
ATCATTCAGA TATGGGACAA TGACAAGTTT TCTCTGGATG ACTACTTGGG TTTCCTAGAA 
CTTGACTTGC GTCACACGAT CATTCCTGCA AAATCACCAG AGAAATGCAG ATTGGACATG 
ATTCCGGACC TCAAAGCCAT GAACCCCCTT AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG 
AAGTCCATGA AAGGATGGTG GCCATGCTAC GCAGAGAAAG ATGGCGCCCG CGTAATGGCT 
GGGAAAGTGG AGATGACATT GGAAATCCTC AACGAGAAGG AGGCCGACGA GAGGCCAGCC 
GGGAAGGGGC GGGACGAACC CAACATGAAC CCCAAGCTGG ACTTACCAAA TCGACCAGAA 
ACCTCCTTCC TCTGGTTCAC CAACCCATGC AAGACCATGA AGTTCATCGT GTGGCGCCGC 
TTTAAGTGGG TCATCATCGG CTTGCTGTTC CTGCTTATCC TGCTGCTCTT CGTGGCCGTG 
CTCCTCTACT CTTTGCCGAA CTATTTGTCA ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_013451.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_038479.1 Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.

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CloneID	OHu28602
Clone ID Related Accession (Same CDS sequence)	NM_013451.4 , NM_013451.3
Accession Version	NM_013451.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	myoferlin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360229.16 and AL365364.19. On Nov 23, 2018 this sequence version replaced NM_013451.3. Transcript Variant: This variant (1) encodes the full-length isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182316.1, SRR1660807.17015.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359263.9/ ENSP00000352208.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGA CAGAAAAGGA GGAAGAAATC GTGGACTGGT GGAGTAAATT TTATGCTTCC 
TCAGGGGAAC ATGAAAAATG CGGACAGTAT ATTCAGAAAG GCTATTCCAA GCTCAAGATA 
TATAATTGTG AACTAGAAAA TGTAGCAGAA TTTGAGGGCC TGACAGACTT CTCAGATACG 
TTCAAGTTGT ACCGAGGCAA GTCGGATGAA AATGAAGATC CTTCTGTGGT TGGAGAGTTT 
AAGGGCTCCT TTCGGATCTA CCCTCTGCCG GATGACCCCA GCGTGCCAGC CCCTCCCAGA 
CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTCAT TGAAGACCGA GATCACTACA TTCCCAACAC TCTCAACCCA 
GTCTTTGGCA GGATGTACGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA CCTGAAAATT 
TCTGTCTATG ATTATGACAC CTTTACCCGG GATGAAAAAG TAGGAGAAAC AATTATTGAT 
CTGGAAAACC GATTCCTTTC CCGCTTTGGG TCCCACTGCG GCATACCAGA GGAGTACTGT 
GTTTCTGGAG TCAATACCTG GCGAGATCAA CTGAGACCAA CACAGCTGCT TCAAAATGTC 
GCCAGATTCA AAGGCTTCCC ACAACCCATC CTTTCCGAAG ATGGGAGTAG AATCAGATAT 
GGAGGACGAG ACTACAGCTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC 
GGGGCCCCTG AAGAGCGGCT TGCTCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAAA CAAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATCA TCTGGAACAC CAAGGACGTT 
ATCTTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGTG ACATCTACGT CAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGATC TTTGGATGGT 
GAAGGGAATT TTAACTGGCG ATTTGTTTTC CCGTTTGACT ACCTTCCAGC CGAACAACTC 
TGTATCGTTG CGAAAAAAGA GCATTTCTGG AGTATTGACC AAACGGAATT TCGAATCCCA 
CCCAGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTAGAAC TTGACTTGCG TCACACGATC ATTCCTGCAA AATCACCAGA GAAATGCAGA 
TTGGACATGA TTCCGGACCT CAAAGCCATG AACCCCCTTA AAGCCAAGAC AGCCTCCCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCATGCTACG CAGAGAAAGA TGGCGCCCGC 
GTAATGGCTG GGAAAGTGGA GATGACATTG GAAATCCTCA ACGAGAAGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GGACGAACCC AACATGAACC CCAAGCTGGA CTTACCAAAT 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGCT TTAAGTGGGT CATCATCGGC TTGCTGTTCC TGCTTATCCT GCTGCTCTTC 
GTGGCCGTGC TCCTCTACTC TTTGCCGAAC TATTTGTCAA TGAAGATTGT AAAGCCAAAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038479.1
CDS	141..6326
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	NM_013451.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013451.4

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGA CAGAAAAGGA GGAAGAAATC GTGGACTGGT GGAGTAAATT TTATGCTTCC 
TCAGGGGAAC ATGAAAAATG CGGACAGTAT ATTCAGAAAG GCTATTCCAA GCTCAAGATA 
TATAATTGTG AACTAGAAAA TGTAGCAGAA TTTGAGGGCC TGACAGACTT CTCAGATACG 
TTCAAGTTGT ACCGAGGCAA GTCGGATGAA AATGAAGATC CTTCTGTGGT TGGAGAGTTT 
AAGGGCTCCT TTCGGATCTA CCCTCTGCCG GATGACCCCA GCGTGCCAGC CCCTCCCAGA 
CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTCAT TGAAGACCGA GATCACTACA TTCCCAACAC TCTCAACCCA 
GTCTTTGGCA GGATGTACGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA CCTGAAAATT 
TCTGTCTATG ATTATGACAC CTTTACCCGG GATGAAAAAG TAGGAGAAAC AATTATTGAT 
CTGGAAAACC GATTCCTTTC CCGCTTTGGG TCCCACTGCG GCATACCAGA GGAGTACTGT 
GTTTCTGGAG TCAATACCTG GCGAGATCAA CTGAGACCAA CACAGCTGCT TCAAAATGTC 
GCCAGATTCA AAGGCTTCCC ACAACCCATC CTTTCCGAAG ATGGGAGTAG AATCAGATAT 
GGAGGACGAG ACTACAGCTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC 
GGGGCCCCTG AAGAGCGGCT TGCTCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAAA CAAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATCA TCTGGAACAC CAAGGACGTT 
ATCTTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGTG ACATCTACGT CAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGATC TTTGGATGGT 
GAAGGGAATT TTAACTGGCG ATTTGTTTTC CCGTTTGACT ACCTTCCAGC CGAACAACTC 
TGTATCGTTG CGAAAAAAGA GCATTTCTGG AGTATTGACC AAACGGAATT TCGAATCCCA 
CCCAGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTAGAAC TTGACTTGCG TCACACGATC ATTCCTGCAA AATCACCAGA GAAATGCAGA 
TTGGACATGA TTCCGGACCT CAAAGCCATG AACCCCCTTA AAGCCAAGAC AGCCTCCCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCATGCTACG CAGAGAAAGA TGGCGCCCGC 
GTAATGGCTG GGAAAGTGGA GATGACATTG GAAATCCTCA ACGAGAAGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GGACGAACCC AACATGAACC CCAAGCTGGA CTTACCAAAT 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGCT TTAAGTGGGT CATCATCGGC TTGCTGTTCC TGCTTATCCT GCTGCTCTTC 
GTGGCCGTGC TCCTCTACTC TTTGCCGAAC TATTTGTCAA TGAAGATTGT AAAGCCAAAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_013451.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_038479.1 Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.

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CloneID	OHu28602
Clone ID Related Accession (Same CDS sequence)	NM_013451.4 , NM_013451.3
Accession Version	NM_013451.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033616.1, AF182316.1, AF207990.1 and AL096713.1. On Dec 2, 2008 this sequence version replaced NM_013451.2. Transcript Variant: This variant (1) encodes the full-length isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182316.1, SRR1660807.17015.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGA CAGAAAAGGA GGAAGAAATC GTGGACTGGT GGAGTAAATT TTATGCTTCC 
TCAGGGGAAC ATGAAAAATG CGGACAGTAT ATTCAGAAAG GCTATTCCAA GCTCAAGATA 
TATAATTGTG AACTAGAAAA TGTAGCAGAA TTTGAGGGCC TGACAGACTT CTCAGATACG 
TTCAAGTTGT ACCGAGGCAA GTCGGATGAA AATGAAGATC CTTCTGTGGT TGGAGAGTTT 
AAGGGCTCCT TTCGGATCTA CCCTCTGCCG GATGACCCCA GCGTGCCAGC CCCTCCCAGA 
CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTCAT TGAAGACCGA GATCACTACA TTCCCAACAC TCTCAACCCA 
GTCTTTGGCA GGATGTACGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA CCTGAAAATT 
TCTGTCTATG ATTATGACAC CTTTACCCGG GATGAAAAAG TAGGAGAAAC AATTATTGAT 
CTGGAAAACC GATTCCTTTC CCGCTTTGGG TCCCACTGCG GCATACCAGA GGAGTACTGT 
GTTTCTGGAG TCAATACCTG GCGAGATCAA CTGAGACCAA CACAGCTGCT TCAAAATGTC 
GCCAGATTCA AAGGCTTCCC ACAACCCATC CTTTCCGAAG ATGGGAGTAG AATCAGATAT 
GGAGGACGAG ACTACAGCTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC 
GGGGCCCCTG AAGAGCGGCT TGCTCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAAA CAAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATCA TCTGGAACAC CAAGGACGTT 
ATCTTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGTG ACATCTACGT CAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGATC TTTGGATGGT 
GAAGGGAATT TTAACTGGCG ATTTGTTTTC CCGTTTGACT ACCTTCCAGC CGAACAACTC 
TGTATCGTTG CGAAAAAAGA GCATTTCTGG AGTATTGACC AAACGGAATT TCGAATCCCA 
CCCAGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTAGAAC TTGACTTGCG TCACACGATC ATTCCTGCAA AATCACCAGA GAAATGCAGA 
TTGGACATGA TTCCGGACCT CAAAGCCATG AACCCCCTTA AAGCCAAGAC AGCCTCCCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCATGCTACG CAGAGAAAGA TGGCGCCCGC 
GTAATGGCTG GGAAAGTGGA GATGACATTG GAAATCCTCA ACGAGAAGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GGACGAACCC AACATGAACC CCAAGCTGGA CTTACCAAAT 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGCT TTAAGTGGGT CATCATCGGC TTGCTGTTCC TGCTTATCCT GCTGCTCTTC 
GTGGCCGTGC TCCTCTACTC TTTGCCGAAC TATTTGTCAA TGAAGATTGT AAAGCCAAAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038479.1
CDS	124..6309
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	NM_013451.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013451.3

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGA CAGAAAAGGA GGAAGAAATC GTGGACTGGT GGAGTAAATT TTATGCTTCC 
TCAGGGGAAC ATGAAAAATG CGGACAGTAT ATTCAGAAAG GCTATTCCAA GCTCAAGATA 
TATAATTGTG AACTAGAAAA TGTAGCAGAA TTTGAGGGCC TGACAGACTT CTCAGATACG 
TTCAAGTTGT ACCGAGGCAA GTCGGATGAA AATGAAGATC CTTCTGTGGT TGGAGAGTTT 
AAGGGCTCCT TTCGGATCTA CCCTCTGCCG GATGACCCCA GCGTGCCAGC CCCTCCCAGA 
CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT 
CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA 
ACACTGGGCA AAAAAGTCAT TGAAGACCGA GATCACTACA TTCCCAACAC TCTCAACCCA 
GTCTTTGGCA GGATGTACGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA CCTGAAAATT 
TCTGTCTATG ATTATGACAC CTTTACCCGG GATGAAAAAG TAGGAGAAAC AATTATTGAT 
CTGGAAAACC GATTCCTTTC CCGCTTTGGG TCCCACTGCG GCATACCAGA GGAGTACTGT 
GTTTCTGGAG TCAATACCTG GCGAGATCAA CTGAGACCAA CACAGCTGCT TCAAAATGTC 
GCCAGATTCA AAGGCTTCCC ACAACCCATC CTTTCCGAAG ATGGGAGTAG AATCAGATAT 
GGAGGACGAG ACTACAGCTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC 
GGGGCCCCTG AAGAGCGGCT TGCTCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAAA CAAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGAAAACTT 
CAGATGTGGG TGGATGTTTT CCCCAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC 
ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATCA TCTGGAACAC CAAGGACGTT 
ATCTTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGTG ACATCTACGT CAAAGGCTGG 
ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGATC TTTGGATGGT 
GAAGGGAATT TTAACTGGCG ATTTGTTTTC CCGTTTGACT ACCTTCCAGC CGAACAACTC 
TGTATCGTTG CGAAAAAAGA GCATTTCTGG AGTATTGACC AAACGGAATT TCGAATCCCA 
CCCAGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT 
TTCCTAGAAC TTGACTTGCG TCACACGATC ATTCCTGCAA AATCACCAGA GAAATGCAGA 
TTGGACATGA TTCCGGACCT CAAAGCCATG AACCCCCTTA AAGCCAAGAC AGCCTCCCTC 
TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCATGCTACG CAGAGAAAGA TGGCGCCCGC 
GTAATGGCTG GGAAAGTGGA GATGACATTG GAAATCCTCA ACGAGAAGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GGACGAACCC AACATGAACC CCAAGCTGGA CTTACCAAAT 
CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGCA AGACCATGAA GTTCATCGTG 
TGGCGCCGCT TTAAGTGGGT CATCATCGGC TTGCTGTTCC TGCTTATCCT GCTGCTCTTC 
GTGGCCGTGC TCCTCTACTC TTTGCCGAAC TATTTGTCAA TGAAGATTGT AAAGCCAAAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_133337.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_579899.1 Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.

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CloneID	OHu28607
Clone ID Related Accession (Same CDS sequence)	NM_133337.2 , NM_133337.3
Accession Version	NM_133337.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	myoferlin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033616.1, AF207990.1, BC052617.1, AF182316.1 and AL096713.1. On Dec 2, 2008 this sequence version replaced NM_133337.1. Transcript Variant: This variant (2) lacks a 39 nt coding region as compared to transcript variant 1. As a result, variant 2 encodes an isoform (b) that lacks an internal 13 aa segment as compared to isoform a, which is encoded by variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF207990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG ACAGAAAAGG AGGAAGAAAT CGTGGACTGG 
TGGAGTAAAT TTTATGCTTC CTCAGGGGAA CATGAAAAAT GCGGACAGTA TATTCAGAAA 
GGCTATTCCA AGCTCAAGAT ATATAATTGT GAACTAGAAA ATGTAGCAGA ATTTGAGGGC 
CTGACAGACT TCTCAGATAC GTTCAAGTTG TACCGAGGCA AGTCGGATGA AAATGAAGAT 
CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC TTTCGGATCT ACCCTCTGCC GGATGACCCC 
AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTCA TTGAAGACCG AGATCACTAC 
ATTCCCAACA CTCTCAACCC AGTCTTTGGC AGGATGTACG AACTGAGCTG CTACTTACCT 
CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT GATTATGACA CCTTTACCCG GGATGAAAAA 
GTAGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGCTTTGG GTCCCACTGC 
GGCATACCAG AGGAGTACTG TGTTTCTGGA GTCAATACCT GGCGAGATCA ACTGAGACCA 
ACACAGCTGC TTCAAAATGT CGCCAGATTC AAAGGCTTCC CACAACCCAT CCTTTCCGAA 
GATGGGAGTA GAATCAGATA TGGAGGACGA GACTACAGCT TGGATGAATT TGAAGCCAAC 
AAAATCCTGC ACCAGCACCT CGGGGCCCCT GAAGAGCGGC TTGCTCTTCA CATCCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAA ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AACATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTTT TCCCCAAGAG TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATC 
ATCTGGAACA CCAAGGACGT TATCTTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGT 
GACATCTACG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGAT CTTTGGATGG TGAAGGGAAT TTTAACTGGC GATTTGTTTT CCCGTTTGAC 
TACCTTCCAG CCGAACAACT CTGTATCGTT GCGAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACGGAAT TTCGAATCCC ACCCAGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTAGAA CTTGACTTGC GTCACACGAT CATTCCTGCA 
AAATCACCAG AGAAATGCAG ATTGGACATG ATTCCGGACC TCAAAGCCAT GAACCCCCTT 
AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCATGCTAC 
GCAGAGAAAG ATGGCGCCCG CGTAATGGCT GGGAAAGTGG AGATGACATT GGAAATCCTC 
AACGAGAAGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGGACGAACC CAACATGAAC 
CCCAAGCTGG ACTTACCAAA TCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGC TTTAAGTGGG TCATCATCGG CTTGCTGTTC 
CTGCTTATCC TGCTGCTCTT CGTGGCCGTG CTCCTCTACT CTTTGCCGAA CTATTTGTCA 
ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579899.1
CDS	124..6270
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	NM_133337.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133337.2

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG ACAGAAAAGG AGGAAGAAAT CGTGGACTGG 
TGGAGTAAAT TTTATGCTTC CTCAGGGGAA CATGAAAAAT GCGGACAGTA TATTCAGAAA 
GGCTATTCCA AGCTCAAGAT ATATAATTGT GAACTAGAAA ATGTAGCAGA ATTTGAGGGC 
CTGACAGACT TCTCAGATAC GTTCAAGTTG TACCGAGGCA AGTCGGATGA AAATGAAGAT 
CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC TTTCGGATCT ACCCTCTGCC GGATGACCCC 
AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTCA TTGAAGACCG AGATCACTAC 
ATTCCCAACA CTCTCAACCC AGTCTTTGGC AGGATGTACG AACTGAGCTG CTACTTACCT 
CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT GATTATGACA CCTTTACCCG GGATGAAAAA 
GTAGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGCTTTGG GTCCCACTGC 
GGCATACCAG AGGAGTACTG TGTTTCTGGA GTCAATACCT GGCGAGATCA ACTGAGACCA 
ACACAGCTGC TTCAAAATGT CGCCAGATTC AAAGGCTTCC CACAACCCAT CCTTTCCGAA 
GATGGGAGTA GAATCAGATA TGGAGGACGA GACTACAGCT TGGATGAATT TGAAGCCAAC 
AAAATCCTGC ACCAGCACCT CGGGGCCCCT GAAGAGCGGC TTGCTCTTCA CATCCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAA ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AACATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTTT TCCCCAAGAG TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATC 
ATCTGGAACA CCAAGGACGT TATCTTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGT 
GACATCTACG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGAT CTTTGGATGG TGAAGGGAAT TTTAACTGGC GATTTGTTTT CCCGTTTGAC 
TACCTTCCAG CCGAACAACT CTGTATCGTT GCGAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACGGAAT TTCGAATCCC ACCCAGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTAGAA CTTGACTTGC GTCACACGAT CATTCCTGCA 
AAATCACCAG AGAAATGCAG ATTGGACATG ATTCCGGACC TCAAAGCCAT GAACCCCCTT 
AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCATGCTAC 
GCAGAGAAAG ATGGCGCCCG CGTAATGGCT GGGAAAGTGG AGATGACATT GGAAATCCTC 
AACGAGAAGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGGACGAACC CAACATGAAC 
CCCAAGCTGG ACTTACCAAA TCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGC TTTAAGTGGG TCATCATCGG CTTGCTGTTC 
CTGCTTATCC TGCTGCTCTT CGTGGCCGTG CTCCTCTACT CTTTGCCGAA CTATTTGTCA 
ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( NM_133337.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_579899.1 Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.

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CloneID	OHu28607
Clone ID Related Accession (Same CDS sequence)	NM_133337.2 , NM_133337.3
Accession Version	NM_133337.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	myoferlin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360229.16 and AL365364.19. On Jun 1, 2019 this sequence version replaced NM_133337.2. Transcript Variant: This variant (2) lacks a 39 nt coding region as compared to transcript variant 1. As a result, variant 2 encodes an isoform (b) that lacks an internal 13 aa segment as compared to isoform a, which is encoded by variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF207990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG ACAGAAAAGG AGGAAGAAAT CGTGGACTGG 
TGGAGTAAAT TTTATGCTTC CTCAGGGGAA CATGAAAAAT GCGGACAGTA TATTCAGAAA 
GGCTATTCCA AGCTCAAGAT ATATAATTGT GAACTAGAAA ATGTAGCAGA ATTTGAGGGC 
CTGACAGACT TCTCAGATAC GTTCAAGTTG TACCGAGGCA AGTCGGATGA AAATGAAGAT 
CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC TTTCGGATCT ACCCTCTGCC GGATGACCCC 
AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTCA TTGAAGACCG AGATCACTAC 
ATTCCCAACA CTCTCAACCC AGTCTTTGGC AGGATGTACG AACTGAGCTG CTACTTACCT 
CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT GATTATGACA CCTTTACCCG GGATGAAAAA 
GTAGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGCTTTGG GTCCCACTGC 
GGCATACCAG AGGAGTACTG TGTTTCTGGA GTCAATACCT GGCGAGATCA ACTGAGACCA 
ACACAGCTGC TTCAAAATGT CGCCAGATTC AAAGGCTTCC CACAACCCAT CCTTTCCGAA 
GATGGGAGTA GAATCAGATA TGGAGGACGA GACTACAGCT TGGATGAATT TGAAGCCAAC 
AAAATCCTGC ACCAGCACCT CGGGGCCCCT GAAGAGCGGC TTGCTCTTCA CATCCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAA ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AACATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTTT TCCCCAAGAG TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATC 
ATCTGGAACA CCAAGGACGT TATCTTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGT 
GACATCTACG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGAT CTTTGGATGG TGAAGGGAAT TTTAACTGGC GATTTGTTTT CCCGTTTGAC 
TACCTTCCAG CCGAACAACT CTGTATCGTT GCGAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACGGAAT TTCGAATCCC ACCCAGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTAGAA CTTGACTTGC GTCACACGAT CATTCCTGCA 
AAATCACCAG AGAAATGCAG ATTGGACATG ATTCCGGACC TCAAAGCCAT GAACCCCCTT 
AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCATGCTAC 
GCAGAGAAAG ATGGCGCCCG CGTAATGGCT GGGAAAGTGG AGATGACATT GGAAATCCTC 
AACGAGAAGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGGACGAACC CAACATGAAC 
CCCAAGCTGG ACTTACCAAA TCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGC TTTAAGTGGG TCATCATCGG CTTGCTGTTC 
CTGCTTATCC TGCTGCTCTT CGTGGCCGTG CTCCTCTACT CTTTGCCGAA CTATTTGTCA 
ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579899.1
CDS	141..6287
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	NM_133337.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133337.3

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG ACAGAAAAGG AGGAAGAAAT CGTGGACTGG 
TGGAGTAAAT TTTATGCTTC CTCAGGGGAA CATGAAAAAT GCGGACAGTA TATTCAGAAA 
GGCTATTCCA AGCTCAAGAT ATATAATTGT GAACTAGAAA ATGTAGCAGA ATTTGAGGGC 
CTGACAGACT TCTCAGATAC GTTCAAGTTG TACCGAGGCA AGTCGGATGA AAATGAAGAT 
CCTTCTGTGG TTGGAGAGTT TAAGGGCTCC TTTCGGATCT ACCCTCTGCC GGATGACCCC 
AGCGTGCCAG CCCCTCCCAG ACAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC 
ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG 
TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTCA TTGAAGACCG AGATCACTAC 
ATTCCCAACA CTCTCAACCC AGTCTTTGGC AGGATGTACG AACTGAGCTG CTACTTACCT 
CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT GATTATGACA CCTTTACCCG GGATGAAAAA 
GTAGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGCTTTGG GTCCCACTGC 
GGCATACCAG AGGAGTACTG TGTTTCTGGA GTCAATACCT GGCGAGATCA ACTGAGACCA 
ACACAGCTGC TTCAAAATGT CGCCAGATTC AAAGGCTTCC CACAACCCAT CCTTTCCGAA 
GATGGGAGTA GAATCAGATA TGGAGGACGA GACTACAGCT TGGATGAATT TGAAGCCAAC 
AAAATCCTGC ACCAGCACCT CGGGGCCCCT GAAGAGCGGC TTGCTCTTCA CATCCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAA ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AACATTTCCC AGGGAAAACT TCAGATGTGG GTGGATGTTT TCCCCAAGAG TTTGGGGCCA 
CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATC 
ATCTGGAACA CCAAGGACGT TATCTTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGT 
GACATCTACG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC 
CATTACAGAT CTTTGGATGG TGAAGGGAAT TTTAACTGGC GATTTGTTTT CCCGTTTGAC 
TACCTTCCAG CCGAACAACT CTGTATCGTT GCGAAAAAAG AGCATTTCTG GAGTATTGAC 
CAAACGGAAT TTCGAATCCC ACCCAGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCTCTGGATG ACTACTTGGG TTTCCTAGAA CTTGACTTGC GTCACACGAT CATTCCTGCA 
AAATCACCAG AGAAATGCAG ATTGGACATG ATTCCGGACC TCAAAGCCAT GAACCCCCTT 
AAAGCCAAGA CAGCCTCCCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCATGCTAC 
GCAGAGAAAG ATGGCGCCCG CGTAATGGCT GGGAAAGTGG AGATGACATT GGAAATCCTC 
AACGAGAAGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGGACGAACC CAACATGAAC 
CCCAAGCTGG ACTTACCAAA TCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGC 
AAGACCATGA AGTTCATCGT GTGGCGCCGC TTTAAGTGGG TCATCATCGG CTTGCTGTTC 
CTGCTTATCC TGCTGCTCTT CGTGGCCGTG CTCCTCTACT CTTTGCCGAA CTATTTGTCA 
ATGAAGATTG TAAAGCCAAA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_005269693.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269750.1 Homo sapiens myoferlin (MYOF), transcript variant X1, mRNA.

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CloneID	OHu44202
Clone ID Related Accession (Same CDS sequence)	XM_005269693.4
Accession Version	XM_005269693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269693.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGGG AAAACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAGTTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATCATCT GGAACACCAA GGACGTTATC 
TTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGTGACA TCTACGTCAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGATCTTT GGATGGTGAA 
GGGAATTTTA ACTGGCGATT TGTTTTCCCG TTTGACTACC TTCCAGCCGA ACAACTCTGT 
ATCGTTGCGA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CGGAATTTCG AATCCCACCC 
AGGCTGATCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTAGAACTTG ACTTGCGTCA CACGATCATT CCTGCAAAAT CACCAGAGAA ATGCAGATTG 
GACATGATTC CGGACCTCAA AGCCATGAAC CCCCTTAAAG CCAAGACAGC CTCCCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCA TGCTACGCAG AGAAAGATGG CGCCCGCGTA 
ATGGCTGGGA AAGTGGAGAT GACATTGGAA ATCCTCAACG AGAAGGAGGC CGACGAGAGG 
CCAGCCGGGA AGGGGCGGGA CGAACCCAAC ATGAACCCCA AGCTGGACTT ACCAAATCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCATGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGCTTTA AGTGGGTCAT CATCGGCTTG CTGTTCCTGC TTATCCTGCT GCTCTTCGTG 
GCCGTGCTCC TCTACTCTTT GCCGAACTAT TTGTCAATGA AGATTGTAAA GCCAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269750.1
CDS	303..6545
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_005269693.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269693.4

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCTTC GGGAACTGGG 
GCTGCATCAT ATACAGTAAA CACAGGAGAA ACAGAGGTAG GCTTTGTTCC AACGTTTGGA 
CCTTGTTACC TGAATCTTTA TGGAAGCCCC AGAGAGTACA CGGGATTCCC AGACCCCTAT 
GATGAGCTGA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGGAT CTTGGTTGAA 
TTAGCCACTT TTCTTGAGAA GACACCACCA GATAAAAAGC TTGAGCCCAT TTCAAATGAT 
GACCTGCTGG TTGTTGAGAA ATACCAGCGA AGGCGGAAGT ACAGCCTGTC TGCCGTGTTT 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA CACCACCTGT AAGCCTTTGG CATCAACAAC TCAGTACAGC 
CGTGCTGTAT TTGATGGCAA CTACTATTAT TACTTGCCTT GGGCCCACAC CAAGCCAGTT 
GTTACCCTGA CTTCATACTG GGAGGATATT AGTCATCGCC TGGATGCGGT GAACACTCTC 
CTAGCTATGG CAGAACGGCT GCAAACAAAT ATAGAAGCTC TAAAATCAGG GATACAAGGT 
AAAATTCCTG CAAACCAGCT GGCTGAATTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACGTTGCC TCTCACAGAA GGAAAAGCCA ACGTCACAGT TCTCGATACT 
CAGATCCGAA AGCTGCGGTC CAGGTCTCTC TCCCAAATAC ATGAGGCGGC TGTGAGGATG 
AGGTCGGAAG CCACAGATGT GAAGTCCACA CTGGCAGAAA TTGAGGACTG GCTTGATAAA 
TTAATGCAGC TGACTGAAGA GCCACAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGGGGAGAGA AGAGACTGGC CTATGCACGA ATTCCCGCAC ATCAGGTCTT GTACTCCACC 
AGTGGTGAGA ATGCATCTGG AAAATACTGT GGGAAAACCC AAACCATCTT TCTGAAGTAT 
CCACAGGAGA AAAACAACGG GCCAAAGGTG CCTGTGGAGT TGCGAGTGAA CATCTGGCTA 
GGCTTAAGTG CTGTGGAGAA GAAGTTTAAC AGCTTCGCAG AAGGAACTTT CACCGTCTTT 
GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGTACTTC TGGATTAGTA 
GGACGTCATA AGTTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTTTTTCTG 
CCTCCAAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG 
ACTGAGGCAG ATGCAGGTCA CACGGAGTTC ACTGATGAAG TCTATCAGAA CGAGAGCCGC 
TACCCCGGGG GCGACTGGAA GCCGGCCGAG GACACCTACA CGGATGCGAA CGGCGATAAA 
GCAGCATCAC CCAGCGAGTT GACTTGTCCT CCAGGTTGGG AATGGGAAGA TGATGCATGG 
TCTTATGACA TAAATCGAGC GGTGGATGAG AAAGGCTGGG AATATGGAAT CACCATTCCT 
CCTGATCATA AGCCCAAATC CTGGGTTGCA GCAGAGAAAA TGTACCACAC TCATAGACGG 
CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAGA CTGCTTCAAG CACCGCAAGG 
GCCATGGAGG AATTGCAAGA CCAAGAGGGC TGGGAATATG CTTCTCTAAT TGGCTGGAAA 
TTTCACTGGA AACAACGTAG TTCAGATACC TTCCGCCGCA GACGCTGGAG GAGAAAAATG 
GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGTGC CCTTGGGGCA 
GACACTACCG AAGATGGGGA TGAGAAGAGC CTGGAGAAAC AGAAGCACAG TGCCACCACT 
GTGTTCGGAG CAAACACCCC CATTGTTTCC TGCAATTTTG ACAGAGTCTA CATCTACCAT 
CTGCGCTGCT ATGTCTATCA AGCCAGAAAC CTCTTGGCTT TAGATAAGGA TAGCTTTTCA 
GATCCATATG CTCATATCTG TTTCCTCCAT CGGAGCAAAA CCACTGAGAT CATCCATTCA 
ACCCTGAATC CCACGTGGGA CCAAACAATT ATATTCGATG AAGTTGAAAT CTATGGGGAA 
CCCCAAACAG TTCTACAGAA TCCACCCAAA GTTATCATGG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATTTTCTCTC CTGTGGTGAA ACTGAACTCA 
GAAATGGACA TCACACCCAA ACTTCTCTGG CACCCAGTAA TGAATGGAGA CAAAGCCTGC 
GGGGATGTTC TTGTAACTGC AGAGCTGATT CTGAGGGGCA AGGATGGCTC CAACCTTCCC 
ATTCTTCCCC CTCAAAGGGC GCCAAATCTA TACATGGTCC CCCAGGGGAT CAGGCCTGTG 
GTCCAGCTCA CTGCCATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTTCCAG 
ATGGCTTCTA TCACATCCCC CAGTCTTGTT GTGGAGTGTG GAGGAGAAAG GGTGGAATCG 
GTGGTGATCA AAAACCTTAA GAAGACACCC AACTTTCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAGGA ATTGTACATG CCCCCACTGG TGATCAAGGT CATCGACCAC 
AGGCAGTTTG GGCGGAAGCC TGTCGTCGGC CAGTGCACCA TCGAGCGCCT GGACCGCTTT 
CGCTGTGACC CTTATGCAGG GAAAGAGGAC ATCGTCCCAC AGCTCAAAGC CTCCCTTCTG 
TCTGCCCCAC CATGCCGGGA CATCGTTATC GAAATGGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGTAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AGCGACAGTG 
CATCTGACAG AAAAGGAGGA AGAAATCGTG GACTGGTGGA GTAAATTTTA TGCTTCCTCA 
GGGGAACATG AAAAATGCGG ACAGTATATT CAGAAAGGCT ATTCCAAGCT CAAGATATAT 
AATTGTGAAC TAGAAAATGT AGCAGAATTT GAGGGCCTGA CAGACTTCTC AGATACGTTC 
AAGTTGTACC GAGGCAAGTC GGATGAAAAT GAAGATCCTT CTGTGGTTGG AGAGTTTAAG 
GGCTCCTTTC GGATCTACCC TCTGCCGGAT GACCCCAGCG TGCCAGCCCC TCCCAGACAG 
TTTCGGGAAT TACCTGACAG CGTCCCACAG GAATGCACGG TTAGGATTTA CATTGTTCGA 
GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA 
CTGGGCAAAA AAGTCATTGA AGACCGAGAT CACTACATTC CCAACACTCT CAACCCAGTC 
TTTGGCAGGA TGTACGAACT GAGCTGCTAC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACCTT TACCCGGGAT GAAAAAGTAG GAGAAACAAT TATTGATCTG 
GAAAACCGAT TCCTTTCCCG CTTTGGGTCC CACTGCGGCA TACCAGAGGA GTACTGTGTT 
TCTGGAGTCA ATACCTGGCG AGATCAACTG AGACCAACAC AGCTGCTTCA AAATGTCGCC 
AGATTCAAAG GCTTCCCACA ACCCATCCTT TCCGAAGATG GGAGTAGAAT CAGATATGGA 
GGACGAGACT ACAGCTTGGA TGAATTTGAA GCCAACAAAA TCCTGCACCA GCACCTCGGG 
GCCCCTGAAG AGCGGCTTGC TCTTCACATC CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAAACAA GGACTTTGCA CAGCACCTTC CAGCCCAACA TTTCCCAGGG AAAACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAGAGTTTG GGGCCACCAG GCCCTCCTTT CAACATCACA 
CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATCATCT GGAACACCAA GGACGTTATC 
TTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGTGACA TCTACGTCAA AGGCTGGATT 
CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGATCTTT GGATGGTGAA 
GGGAATTTTA ACTGGCGATT TGTTTTCCCG TTTGACTACC TTCCAGCCGA ACAACTCTGT 
ATCGTTGCGA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CGGAATTTCG AATCCCACCC 
AGGCTGATCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC 
CTAGAACTTG ACTTGCGTCA CACGATCATT CCTGCAAAAT CACCAGAGAA ATGCAGATTG 
GACATGATTC CGGACCTCAA AGCCATGAAC CCCCTTAAAG CCAAGACAGC CTCCCTCTTT 
GAGCAGAAGT CCATGAAAGG ATGGTGGCCA TGCTACGCAG AGAAAGATGG CGCCCGCGTA 
ATGGCTGGGA AAGTGGAGAT GACATTGGAA ATCCTCAACG AGAAGGAGGC CGACGAGAGG 
CCAGCCGGGA AGGGGCGGGA CGAACCCAAC ATGAACCCCA AGCTGGACTT ACCAAATCGA 
CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCATGCAAGA CCATGAAGTT CATCGTGTGG 
CGCCGCTTTA AGTGGGTCAT CATCGGCTTG CTGTTCCTGC TTATCCTGCT GCTCTTCGTG 
GCCGTGCTCC TCTACTCTTT GCCGAACTAT TTGTCAATGA AGATTGTAAA GCCAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_005269694.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269751.1 Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.

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CloneID	OHu44203
Clone ID Related Accession (Same CDS sequence)	XM_005269694.4 , XM_005269694.5
Accession Version	XM_005269694.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269694.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGTA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAGCGACAGT GCATCTGACA GAAAAGGAGG AAGAAATCGT GGACTGGTGG 
AGTAAATTTT ATGCTTCCTC AGGGGAACAT GAAAAATGCG GACAGTATAT TCAGAAAGGC 
TATTCCAAGC TCAAGATATA TAATTGTGAA CTAGAAAATG TAGCAGAATT TGAGGGCCTG 
ACAGACTTCT CAGATACGTT CAAGTTGTAC CGAGGCAAGT CGGATGAAAA TGAAGATCCT 
TCTGTGGTTG GAGAGTTTAA GGGCTCCTTT CGGATCTACC CTCTGCCGGA TGACCCCAGC 
GTGCCAGCCC CTCCCAGACA GTTTCGGGAA TTACCTGACA GCGTCCCACA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTCATTG AAGACCGAGA TCACTACATT 
CCCAACACTC TCAACCCAGT CTTTGGCAGG ATGTACGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACCT TTACCCGGGA TGAAAAAGTA 
GGAGAAACAA TTATTGATCT GGAAAACCGA TTCCTTTCCC GCTTTGGGTC CCACTGCGGC 
ATACCAGAGG AGTACTGTGT TTCTGGAGTC AATACCTGGC GAGATCAACT GAGACCAACA 
CAGCTGCTTC AAAATGTCGC CAGATTCAAA GGCTTCCCAC AACCCATCCT TTCCGAAGAT 
GGGAGTAGAA TCAGATATGG AGGACGAGAC TACAGCTTGG ATGAATTTGA AGCCAACAAA 
ATCCTGCACC AGCACCTCGG GGCCCCTGAA GAGCGGCTTG CTCTTCACAT CCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAAACA AGGACTTTGC ACAGCACCTT CCAGCCCAAC 
ATTTCCCAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAGAGTTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATCATC 
TGGAACACCA AGGACGTTAT CTTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGATCTT TGGATGGTGA AGGGAATTTT AACTGGCGAT TTGTTTTCCC GTTTGACTAC 
CTTCCAGCCG AACAACTCTG TATCGTTGCG AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACGGAATTTC GAATCCCACC CAGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTAGAACTT GACTTGCGTC ACACGATCAT TCCTGCAAAA 
TCACCAGAGA AATGCAGATT GGACATGATT CCGGACCTCA AAGCCATGAA CCCCCTTAAA 
GCCAAGACAG CCTCCCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC ATGCTACGCA 
GAGAAAGATG GCGCCCGCGT AATGGCTGGG AAAGTGGAGA TGACATTGGA AATCCTCAAC 
GAGAAGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGG ACGAACCCAA CATGAACCCC 
AAGCTGGACT TACCAAATCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCATGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGCTTT AAGTGGGTCA TCATCGGCTT GCTGTTCCTG 
CTTATCCTGC TGCTCTTCGT GGCCGTGCTC CTCTACTCTT TGCCGAACTA TTTGTCAATG 
AAGATTGTAA AGCCAAATGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269751.1
CDS	303..6506
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_005269694.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269694.4

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGTA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAGCGACAGT GCATCTGACA GAAAAGGAGG AAGAAATCGT GGACTGGTGG 
AGTAAATTTT ATGCTTCCTC AGGGGAACAT GAAAAATGCG GACAGTATAT TCAGAAAGGC 
TATTCCAAGC TCAAGATATA TAATTGTGAA CTAGAAAATG TAGCAGAATT TGAGGGCCTG 
ACAGACTTCT CAGATACGTT CAAGTTGTAC CGAGGCAAGT CGGATGAAAA TGAAGATCCT 
TCTGTGGTTG GAGAGTTTAA GGGCTCCTTT CGGATCTACC CTCTGCCGGA TGACCCCAGC 
GTGCCAGCCC CTCCCAGACA GTTTCGGGAA TTACCTGACA GCGTCCCACA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTCATTG AAGACCGAGA TCACTACATT 
CCCAACACTC TCAACCCAGT CTTTGGCAGG ATGTACGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACCT TTACCCGGGA TGAAAAAGTA 
GGAGAAACAA TTATTGATCT GGAAAACCGA TTCCTTTCCC GCTTTGGGTC CCACTGCGGC 
ATACCAGAGG AGTACTGTGT TTCTGGAGTC AATACCTGGC GAGATCAACT GAGACCAACA 
CAGCTGCTTC AAAATGTCGC CAGATTCAAA GGCTTCCCAC AACCCATCCT TTCCGAAGAT 
GGGAGTAGAA TCAGATATGG AGGACGAGAC TACAGCTTGG ATGAATTTGA AGCCAACAAA 
ATCCTGCACC AGCACCTCGG GGCCCCTGAA GAGCGGCTTG CTCTTCACAT CCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAAACA AGGACTTTGC ACAGCACCTT CCAGCCCAAC 
ATTTCCCAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAGAGTTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATCATC 
TGGAACACCA AGGACGTTAT CTTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGATCTT TGGATGGTGA AGGGAATTTT AACTGGCGAT TTGTTTTCCC GTTTGACTAC 
CTTCCAGCCG AACAACTCTG TATCGTTGCG AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACGGAATTTC GAATCCCACC CAGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTAGAACTT GACTTGCGTC ACACGATCAT TCCTGCAAAA 
TCACCAGAGA AATGCAGATT GGACATGATT CCGGACCTCA AAGCCATGAA CCCCCTTAAA 
GCCAAGACAG CCTCCCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC ATGCTACGCA 
GAGAAAGATG GCGCCCGCGT AATGGCTGGG AAAGTGGAGA TGACATTGGA AATCCTCAAC 
GAGAAGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGG ACGAACCCAA CATGAACCCC 
AAGCTGGACT TACCAAATCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCATGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGCTTT AAGTGGGTCA TCATCGGCTT GCTGTTCCTG 
CTTATCCTGC TGCTCTTCGT GGCCGTGCTC CTCTACTCTT TGCCGAACTA TTTGTCAATG 
AAGATTGTAA AGCCAAATGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_005269694.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269751.1 Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.

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CloneID	OHu44203
Clone ID Related Accession (Same CDS sequence)	XM_005269694.4 , XM_005269694.5
Accession Version	XM_005269694.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005269694.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGTA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAGCGACAGT GCATCTGACA GAAAAGGAGG AAGAAATCGT GGACTGGTGG 
AGTAAATTTT ATGCTTCCTC AGGGGAACAT GAAAAATGCG GACAGTATAT TCAGAAAGGC 
TATTCCAAGC TCAAGATATA TAATTGTGAA CTAGAAAATG TAGCAGAATT TGAGGGCCTG 
ACAGACTTCT CAGATACGTT CAAGTTGTAC CGAGGCAAGT CGGATGAAAA TGAAGATCCT 
TCTGTGGTTG GAGAGTTTAA GGGCTCCTTT CGGATCTACC CTCTGCCGGA TGACCCCAGC 
GTGCCAGCCC CTCCCAGACA GTTTCGGGAA TTACCTGACA GCGTCCCACA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTCATTG AAGACCGAGA TCACTACATT 
CCCAACACTC TCAACCCAGT CTTTGGCAGG ATGTACGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACCT TTACCCGGGA TGAAAAAGTA 
GGAGAAACAA TTATTGATCT GGAAAACCGA TTCCTTTCCC GCTTTGGGTC CCACTGCGGC 
ATACCAGAGG AGTACTGTGT TTCTGGAGTC AATACCTGGC GAGATCAACT GAGACCAACA 
CAGCTGCTTC AAAATGTCGC CAGATTCAAA GGCTTCCCAC AACCCATCCT TTCCGAAGAT 
GGGAGTAGAA TCAGATATGG AGGACGAGAC TACAGCTTGG ATGAATTTGA AGCCAACAAA 
ATCCTGCACC AGCACCTCGG GGCCCCTGAA GAGCGGCTTG CTCTTCACAT CCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAAACA AGGACTTTGC ACAGCACCTT CCAGCCCAAC 
ATTTCCCAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAGAGTTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATCATC 
TGGAACACCA AGGACGTTAT CTTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGATCTT TGGATGGTGA AGGGAATTTT AACTGGCGAT TTGTTTTCCC GTTTGACTAC 
CTTCCAGCCG AACAACTCTG TATCGTTGCG AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACGGAATTTC GAATCCCACC CAGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTAGAACTT GACTTGCGTC ACACGATCAT TCCTGCAAAA 
TCACCAGAGA AATGCAGATT GGACATGATT CCGGACCTCA AAGCCATGAA CCCCCTTAAA 
GCCAAGACAG CCTCCCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC ATGCTACGCA 
GAGAAAGATG GCGCCCGCGT AATGGCTGGG AAAGTGGAGA TGACATTGGA AATCCTCAAC 
GAGAAGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGG ACGAACCCAA CATGAACCCC 
AAGCTGGACT TACCAAATCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCATGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGCTTT AAGTGGGTCA TCATCGGCTT GCTGTTCCTG 
CTTATCCTGC TGCTCTTCGT GGCCGTGCTC CTCTACTCTT TGCCGAACTA TTTGTCAATG 
AAGATTGTAA AGCCAAATGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269751.1
CDS	304..6507
Translation	MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_005269694.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269694.5

ORF Insert Sequence:

ATGCTGCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AAACGAAATT TGGCAAGCCG 
GATCCTATTG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAATTGAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC 
TTTTCATCTT CCCTTGGGAT TATTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACGG CGACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCCCTGCCG 
TACAAGCTGA TCTCCCTGCT AAATGAAAAA GGGCAAGATA CTGGGGCCAC CATTGACTTG 
GTGATCGGCT ATGATCCGCC TTCTGCTCCA CATCCAAATG ACCTGAGCGG GCCCAGCGTG 
CCAGGCATGG GAGGAGATGG GGAAGAAGAT GAAGGTGATG AAGACAGGTT GGACAATGCA 
GTCAGGGGCC CTGGGCCCAA GGGGCCAGTT GGGACGGTGT CGGAAGCTCA GCTTGCTCGG 
AGGCTCACCA AAGTAAAGAA CAGCCGGCGG ATGCTGTCAA ATAAGCCACA GGACTTCCAG 
ATCCGCGTCC GAGTGATTGA GGGCCGACAG TTAAGTGGTA ACAACATAAG GCCTGTGGTC 
AAAGTTCACG TCTGTGGCCA GACACACCGA ACAAGAATCA AGAGAGGAAA CAACCCTTTT 
TTTGATGAGT TGTTTTTCTA CAATGTCAAC ATGACCCCTT CTGAATTGAT GGATGAGATC 
ATCAGCATCC GGGTTTATAA TTCTCACTCT CTGCGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCATG CTGTCATGAG AAAGTGGCTT 
CTTCTCAATG ACCCGGAAGA TACCAGTTCA GGTTCTAAAG GTTATATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA TGAGCCTCCT CCTGAGAGAC GAGATCGTGA TAATGACAGT 
GATGATGTGG AGAGTAATTT GTTACTCCCT GCTGGCATTG CCCTCCGGTG GGTGACCTTC 
TTGCTGAAAA TCTACCGAGC TGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA 
GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAGAAAAATC TCGTGGATCC TTTTGTAGAA 
GTTTCCTTTG CTGGAAAAAA GGTTTGTACA AACATAATTG AGAAAAATGC AAACCCAGAG 
TGGAATCAGG TCGTCAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTA 
ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTAG TTGGAACAAC ATATCTACAC 
CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGTAA ACACAGGAGA AACAGAGGTA 
GGCTTTGTTC CAACGTTTGG ACCTTGTTAC CTGAATCTTT ATGGAAGCCC CAGAGAGTAC 
ACGGGATTCC CAGACCCCTA TGATGAGCTG AATACTGGAA AGGGGGAAGG AGTTGCCTAC 
AGAGGCAGGA TCTTGGTTGA ATTAGCCACT TTTCTTGAGA AGACACCACC AGATAAAAAG 
CTTGAGCCCA TTTCAAATGA TGACCTGCTG GTTGTTGAGA AATACCAGCG AAGGCGGAAG 
TACAGCCTGT CTGCCGTGTT TCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACCACCTG TAAGCCTTTG 
GCATCAACAA CTCAGTACAG CCGTGCTGTA TTTGATGGCA ACTACTATTA TTACTTGCCT 
TGGGCCCACA CCAAGCCAGT TGTTACCCTG ACTTCATACT GGGAGGATAT TAGTCATCGC 
CTGGATGCGG TGAACACTCT CCTAGCTATG GCAGAACGGC TGCAAACAAA TATAGAAGCT 
CTAAAATCAG GGATACAAGG TAAAATTCCT GCAAACCAGC TGGCTGAATT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACGTTGC CTCTCACAGA AGGAAAAGCC 
AACGTCACAG TTCTCGATAC TCAGATCCGA AAGCTGCGGT CCAGGTCTCT CTCCCAAATA 
CATGAGGCGG CTGTGAGGAT GAGGTCGGAA GCCACAGATG TGAAGTCCAC ACTGGCAGAA 
ATTGAGGACT GGCTTGATAA ATTAATGCAG CTGACTGAAG AGCCACAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGGGGAGAG AAGAGACTGG CCTATGCACG AATTCCCGCA 
CATCAGGTCT TGTACTCCAC CAGTGGTGAG AATGCATCTG GAAAATACTG TGGGAAAACC 
CAAACCATCT TTCTGAAGTA TCCACAGGAG AAAAACAACG GGCCAAAGGT GCCTGTGGAG 
TTGCGAGTGA ACATCTGGCT AGGCTTAAGT GCTGTGGAGA AGAAGTTTAA CAGCTTCGCA 
GAAGGAACTT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTCAT GTTTGGAAAA 
TGGGGTACTT CTGGATTAGT AGGACGTCAT AAGTTTTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGGG AATTTTTTCT GCCTCCAAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT 
GATCCTGAAA GAAGCTTGCT GACTGAGGCA GATGCAGGTC ACACGGAGTT CACTGATGAA 
GTCTATCAGA ACGAGAGCCG CTACCCCGGG GGCGACTGGA AGCCGGCCGA GGACACCTAC 
ACGGATGCGA ACGGCGATAA AGCAGCATCA CCCAGCGAGT TGACTTGTCC TCCAGGTTGG 
GAATGGGAAG ATGATGCATG GTCTTATGAC ATAAATCGAG CGGTGGATGA GAAAGGCTGG 
GAATATGGAA TCACCATTCC TCCTGATCAT AAGCCCAAAT CCTGGGTTGC AGCAGAGAAA 
ATGTACCACA CTCATAGACG GCGAAGGCTG GTCCGAAAAC GCAAGAAAGA TTTAACACAG 
ACTGCTTCAA GCACCGCAAG GGCCATGGAG GAATTGCAAG ACCAAGAGGG CTGGGAATAT 
GCTTCTCTAA TTGGCTGGAA ATTTCACTGG AAACAACGTA GTTCAGATAC CTTCCGCCGC 
AGACGCTGGA GGAGAAAAAT GGCTCCTTCA GAAACACATG GTGCAGCTGC CATCTTTAAA 
CTTGAAGGTG CCCTTGGGGC AGACACTACC GAAGATGGGG ATGAGAAGAG CCTGGAGAAA 
CAGAAGCACA GTGCCACCAC TGTGTTCGGA GCAAACACCC CCATTGTTTC CTGCAATTTT 
GACAGAGTCT ACATCTACCA TCTGCGCTGC TATGTCTATC AAGCCAGAAA CCTCTTGGCT 
TTAGATAAGG ATAGCTTTTC AGATCCATAT GCTCATATCT GTTTCCTCCA TCGGAGCAAA 
ACCACTGAGA TCATCCATTC AACCCTGAAT CCCACGTGGG ACCAAACAAT TATATTCGAT 
GAAGTTGAAA TCTATGGGGA ACCCCAAACA GTTCTACAGA ATCCACCCAA AGTTATCATG 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATTTTCTCT 
CCTGTGGTGA AACTGAACTC AGAAATGGAC ATCACACCCA AACTTCTCTG GCACCCAGTA 
ATGAATGGAG ACAAAGCCTG CGGGGATGTT CTTGTAACTG CAGAGCTGAT TCTGAGGGGC 
AAGGATGGCT CCAACCTTCC CATTCTTCCC CCTCAAAGGG CGCCAAATCT ATACATGGTC 
CCCCAGGGGA TCAGGCCTGT GGTCCAGCTC ACTGCCATTG AGATTCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTTCCA GATGGCTTCT ATCACATCCC CCAGTCTTGT TGTGGAGTGT 
GGAGGAGAAA GGGTGGAATC GGTGGTGATC AAAAACCTTA AGAAGACACC CAACTTTCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AATTGTACAT GCCCCCACTG 
GTGATCAAGG TCATCGACCA CAGGCAGTTT GGGCGGAAGC CTGTCGTCGG CCAGTGCACC 
ATCGAGCGCC TGGACCGCTT TCGCTGTGAC CCTTATGCAG GGAAAGAGGA CATCGTCCCA 
CAGCTCAAAG CCTCCCTTCT GTCTGCCCCA CCATGCCGGG ACATCGTTAT CGAAATGGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGTA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAGCGACAGT GCATCTGACA GAAAAGGAGG AAGAAATCGT GGACTGGTGG 
AGTAAATTTT ATGCTTCCTC AGGGGAACAT GAAAAATGCG GACAGTATAT TCAGAAAGGC 
TATTCCAAGC TCAAGATATA TAATTGTGAA CTAGAAAATG TAGCAGAATT TGAGGGCCTG 
ACAGACTTCT CAGATACGTT CAAGTTGTAC CGAGGCAAGT CGGATGAAAA TGAAGATCCT 
TCTGTGGTTG GAGAGTTTAA GGGCTCCTTT CGGATCTACC CTCTGCCGGA TGACCCCAGC 
GTGCCAGCCC CTCCCAGACA GTTTCGGGAA TTACCTGACA GCGTCCCACA GGAATGCACG 
GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT 
GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTCATTG AAGACCGAGA TCACTACATT 
CCCAACACTC TCAACCCAGT CTTTGGCAGG ATGTACGAAC TGAGCTGCTA CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACCT TTACCCGGGA TGAAAAAGTA 
GGAGAAACAA TTATTGATCT GGAAAACCGA TTCCTTTCCC GCTTTGGGTC CCACTGCGGC 
ATACCAGAGG AGTACTGTGT TTCTGGAGTC AATACCTGGC GAGATCAACT GAGACCAACA 
CAGCTGCTTC AAAATGTCGC CAGATTCAAA GGCTTCCCAC AACCCATCCT TTCCGAAGAT 
GGGAGTAGAA TCAGATATGG AGGACGAGAC TACAGCTTGG ATGAATTTGA AGCCAACAAA 
ATCCTGCACC AGCACCTCGG GGCCCCTGAA GAGCGGCTTG CTCTTCACAT CCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAAACA AGGACTTTGC ACAGCACCTT CCAGCCCAAC 
ATTTCCCAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAGAGTTT GGGGCCACCA 
GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATCATC 
TGGAACACCA AGGACGTTAT CTTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT 
TACAGATCTT TGGATGGTGA AGGGAATTTT AACTGGCGAT TTGTTTTCCC GTTTGACTAC 
CTTCCAGCCG AACAACTCTG TATCGTTGCG AAAAAAGAGC ATTTCTGGAG TATTGACCAA 
ACGGAATTTC GAATCCCACC CAGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCT 
CTGGATGACT ACTTGGGTTT CCTAGAACTT GACTTGCGTC ACACGATCAT TCCTGCAAAA 
TCACCAGAGA AATGCAGATT GGACATGATT CCGGACCTCA AAGCCATGAA CCCCCTTAAA 
GCCAAGACAG CCTCCCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC ATGCTACGCA 
GAGAAAGATG GCGCCCGCGT AATGGCTGGG AAAGTGGAGA TGACATTGGA AATCCTCAAC 
GAGAAGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGG ACGAACCCAA CATGAACCCC 
AAGCTGGACT TACCAAATCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCATGCAAG 
ACCATGAAGT TCATCGTGTG GCGCCGCTTT AAGTGGGTCA TCATCGGCTT GCTGTTCCTG 
CTTATCCTGC TGCTCTTCGT GGCCGTGCTC CTCTACTCTT TGCCGAACTA TTTGTCAATG 
AAGATTGTAA AGCCAAATGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_011539632.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537934.1 Homo sapiens myoferlin (MYOF), transcript variant X3, mRNA.

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CloneID	OHu66943
Clone ID Related Accession (Same CDS sequence)	XM_011539632.2
Accession Version	XM_011539632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTTC CAAACAGTCC TCCAAACAGA ACAGATGAGA AAAAGAAAAC AAAGAAAGTT 
GATAATGAAT TGAACCCTGT CTGGAATGAG ATTTTGGAGT TTGACTTGAG GGGTATACCA 
CTGGACTTTT CATCTTCCCT TGGGATTATT GTGAAAGATT TTGAGACAAT TGGACAAAAT 
AAATTAATTG GCACGGCGAC TGTAGCCCTG AAGGACCTGA CTGGTGACCA GAGCAGATCC 
CTGCCGTACA AGCTGATCTC CCTGCTAAAT GAAAAAGGGC AAGATACTGG GGCCACCATT 
GACTTGGTGA TCGGCTATGA TCCGCCTTCT GCTCCACATC CAAATGACCT GAGCGGGCCC 
AGCGTGCCAG GCATGGGAGG AGATGGGGAA GAAGATGAAG GTGATGAAGA CAGGTTGGAC 
AATGCAGTCA GGGGCCCTGG GCCCAAGGGG CCAGTTGGGA CGGTGTCGGA AGCTCAGCTT 
GCTCGGAGGC TCACCAAAGT AAAGAACAGC CGGCGGATGC TGTCAAATAA GCCACAGGAC 
TTCCAGATCC GCGTCCGAGT GATTGAGGGC CGACAGTTAA GTGGTAACAA CATAAGGCCT 
GTGGTCAAAG TTCACGTCTG TGGCCAGACA CACCGAACAA GAATCAAGAG AGGAAACAAC 
CCTTTTTTTG ATGAGTTGTT TTTCTACAAT GTCAACATGA CCCCTTCTGA ATTGATGGAT 
GAGATCATCA GCATCCGGGT TTATAATTCT CACTCTCTGC GGGCAGATTG TCTGATGGGG 
GAATTTAAGA TTGATGTTGG ATTTGTTTAT GATGAACCTG GCCATGCTGT CATGAGAAAG 
TGGCTTCTTC TCAATGACCC GGAAGATACC AGTTCAGGTT CTAAAGGTTA TATGAAAGTC 
AGCATGTTTG TCCTGGGAAC CGGAGATGAG CCTCCTCCTG AGAGACGAGA TCGTGATAAT 
GACAGTGATG ATGTGGAGAG TAATTTGTTA CTCCCTGCTG GCATTGCCCT CCGGTGGGTG 
ACCTTCTTGC TGAAAATCTA CCGAGCTGAG GACATCCCCC AGATGGATGA TGCCTTCTCA 
CAGACAGTAA AGGAAATATT TGGAGGCAAT GCAGATAAGA AAAATCTCGT GGATCCTTTT 
GTAGAAGTTT CCTTTGCTGG AAAAAAGGTT TGTACAAACA TAATTGAGAA AAATGCAAAC 
CCAGAGTGGA ATCAGGTCGT CAATCTTCAG ATCAAGTTTC CTTCAGTGTG TGAAAAAATA 
AAACTAACAA TATATGACTG GGACCGTCTT ACTAAAAATG ATGTAGTTGG AACAACATAT 
CTACACCTCT CTAAAATTGC TGCCTCTGGT GGGGAAGTGG AAGATTTCTC ATCTTCGGGA 
ACTGGGGCTG CATCATATAC AGTAAACACA GGAGAAACAG AGGTAGGCTT TGTTCCAACG 
TTTGGACCTT GTTACCTGAA TCTTTATGGA AGCCCCAGAG AGTACACGGG ATTCCCAGAC 
CCCTATGATG AGCTGAATAC TGGAAAGGGG GAAGGAGTTG CCTACAGAGG CAGGATCTTG 
GTTGAATTAG CCACTTTTCT TGAGAAGACA CCACCAGATA AAAAGCTTGA GCCCATTTCA 
AATGATGACC TGCTGGTTGT TGAGAAATAC CAGCGAAGGC GGAAGTACAG CCTGTCTGCC 
GTGTTTCATT CAGCCACCAT GTTGCAAGAT GTTGGTGAGG CCATTCAGTT TGAAGTCAGC 
ATTGGGAACT ATGGCAACAA GTTTGACACC ACCTGTAAGC CTTTGGCATC AACAACTCAG 
TACAGCCGTG CTGTATTTGA TGGCAACTAC TATTATTACT TGCCTTGGGC CCACACCAAG 
CCAGTTGTTA CCCTGACTTC ATACTGGGAG GATATTAGTC ATCGCCTGGA TGCGGTGAAC 
ACTCTCCTAG CTATGGCAGA ACGGCTGCAA ACAAATATAG AAGCTCTAAA ATCAGGGATA 
CAAGGTAAAA TTCCTGCAAA CCAGCTGGCT GAATTGTGGC TGAAGCTGAT AGATGAAGTT 
ATAGAAGACA CGAGATACAC GTTGCCTCTC ACAGAAGGAA AAGCCAACGT CACAGTTCTC 
GATACTCAGA TCCGAAAGCT GCGGTCCAGG TCTCTCTCCC AAATACATGA GGCGGCTGTG 
AGGATGAGGT CGGAAGCCAC AGATGTGAAG TCCACACTGG CAGAAATTGA GGACTGGCTT 
GATAAATTAA TGCAGCTGAC TGAAGAGCCA CAGAACAGCA TGCCTGACAT CATCATCTGG 
ATGATCCGGG GAGAGAAGAG ACTGGCCTAT GCACGAATTC CCGCACATCA GGTCTTGTAC 
TCCACCAGTG GTGAGAATGC ATCTGGAAAA TACTGTGGGA AAACCCAAAC CATCTTTCTG 
AAGTATCCAC AGGAGAAAAA CAACGGGCCA AAGGTGCCTG TGGAGTTGCG AGTGAACATC 
TGGCTAGGCT TAAGTGCTGT GGAGAAGAAG TTTAACAGCT TCGCAGAAGG AACTTTCACC 
GTCTTTGCTG AAATGTATGA AAATCAAGCT CTCATGTTTG GAAAATGGGG TACTTCTGGA 
TTAGTAGGAC GTCATAAGTT TTCTGATGTC ACAGGAAAAA TAAAACTCAA GAGGGAATTT 
TTTCTGCCTC CAAAAGGCTG GGAATGGGAA GGAGAGTGGA TAGTTGATCC TGAAAGAAGC 
TTGCTGACTG AGGCAGATGC AGGTCACACG GAGTTCACTG ATGAAGTCTA TCAGAACGAG 
AGCCGCTACC CCGGGGGCGA CTGGAAGCCG GCCGAGGACA CCTACACGGA TGCGAACGGC 
GATAAAGCAG CATCACCCAG CGAGTTGACT TGTCCTCCAG GTTGGGAATG GGAAGATGAT 
GCATGGTCTT ATGACATAAA TCGAGCGGTG GATGAGAAAG GCTGGGAATA TGGAATCACC 
ATTCCTCCTG ATCATAAGCC CAAATCCTGG GTTGCAGCAG AGAAAATGTA CCACACTCAT 
AGACGGCGAA GGCTGGTCCG AAAACGCAAG AAAGATTTAA CACAGACTGC TTCAAGCACC 
GCAAGGGCCA TGGAGGAATT GCAAGACCAA GAGGGCTGGG AATATGCTTC TCTAATTGGC 
TGGAAATTTC ACTGGAAACA ACGTAGTTCA GATACCTTCC GCCGCAGACG CTGGAGGAGA 
AAAATGGCTC CTTCAGAAAC ACATGGTGCA GCTGCCATCT TTAAACTTGA AGGTGCCCTT 
GGGGCAGACA CTACCGAAGA TGGGGATGAG AAGAGCCTGG AGAAACAGAA GCACAGTGCC 
ACCACTGTGT TCGGAGCAAA CACCCCCATT GTTTCCTGCA ATTTTGACAG AGTCTACATC 
TACCATCTGC GCTGCTATGT CTATCAAGCC AGAAACCTCT TGGCTTTAGA TAAGGATAGC 
TTTTCAGATC CATATGCTCA TATCTGTTTC CTCCATCGGA GCAAAACCAC TGAGATCATC 
CATTCAACCC TGAATCCCAC GTGGGACCAA ACAATTATAT TCGATGAAGT TGAAATCTAT 
GGGGAACCCC AAACAGTTCT ACAGAATCCA CCCAAAGTTA TCATGGAACT TTTTGACAAT 
GACCAAGTGG GCAAAGATGA ATTTTTAGGA CGAAGCATTT TCTCTCCTGT GGTGAAACTG 
AACTCAGAAA TGGACATCAC ACCCAAACTT CTCTGGCACC CAGTAATGAA TGGAGACAAA 
GCCTGCGGGG ATGTTCTTGT AACTGCAGAG CTGATTCTGA GGGGCAAGGA TGGCTCCAAC 
CTTCCCATTC TTCCCCCTCA AAGGGCGCCA AATCTATACA TGGTCCCCCA GGGGATCAGG 
CCTGTGGTCC AGCTCACTGC CATTGAGATT CTAGCTTGGG GCTTAAGAAA TATGAAAAAC 
TTCCAGATGG CTTCTATCAC ATCCCCCAGT CTTGTTGTGG AGTGTGGAGG AGAAAGGGTG 
GAATCGGTGG TGATCAAAAA CCTTAAGAAG ACACCCAACT TTCCAAGTTC TGTTCTCTTC 
ATGAAAGTGT TCTTGCCCAA GGAGGAATTG TACATGCCCC CACTGGTGAT CAAGGTCATC 
GACCACAGGC AGTTTGGGCG GAAGCCTGTC GTCGGCCAGT GCACCATCGA GCGCCTGGAC 
CGCTTTCGCT GTGACCCTTA TGCAGGGAAA GAGGACATCG TCCCACAGCT CAAAGCCTCC 
CTTCTGTCTG CCCCACCATG CCGGGACATC GTTATCGAAA TGGAAGACAC CAAACCATTA 
CTGGCTTCTA AGTGCTTAAG CAGTATGTCA ACAGCACTCA GCAAAATGGC TTCTCCAGCG 
ACAGTGCATC TGACAGAAAA GGAGGAAGAA ATCGTGGACT GGTGGAGTAA ATTTTATGCT 
TCCTCAGGGG AACATGAAAA ATGCGGACAG TATATTCAGA AAGGCTATTC CAAGCTCAAG 
ATATATAATT GTGAACTAGA AAATGTAGCA GAATTTGAGG GCCTGACAGA CTTCTCAGAT 
ACGTTCAAGT TGTACCGAGG CAAGTCGGAT GAAAATGAAG ATCCTTCTGT GGTTGGAGAG 
TTTAAGGGCT CCTTTCGGAT CTACCCTCTG CCGGATGACC CCAGCGTGCC AGCCCCTCCC 
AGACAGTTTC GGGAATTACC TGACAGCGTC CCACAGGAAT GCACGGTTAG GATTTACATT 
GTTCGAGGCT TAGAGCTCCA GCCCCAGGAC AACAATGGCC TGTGTGACCC TTACATAAAA 
ATAACACTGG GCAAAAAAGT CATTGAAGAC CGAGATCACT ACATTCCCAA CACTCTCAAC 
CCAGTCTTTG GCAGGATGTA CGAACTGAGC TGCTACTTAC CTCAAGAAAA AGACCTGAAA 
ATTTCTGTCT ATGATTATGA CACCTTTACC CGGGATGAAA AAGTAGGAGA AACAATTATT 
GATCTGGAAA ACCGATTCCT TTCCCGCTTT GGGTCCCACT GCGGCATACC AGAGGAGTAC 
TGTGTTTCTG GAGTCAATAC CTGGCGAGAT CAACTGAGAC CAACACAGCT GCTTCAAAAT 
GTCGCCAGAT TCAAAGGCTT CCCACAACCC ATCCTTTCCG AAGATGGGAG TAGAATCAGA 
TATGGAGGAC GAGACTACAG CTTGGATGAA TTTGAAGCCA ACAAAATCCT GCACCAGCAC 
CTCGGGGCCC CTGAAGAGCG GCTTGCTCTT CACATCCTCA GGACTCAGGG GCTGGTCCCT 
GAGCACGTGG AAACAAGGAC TTTGCACAGC ACCTTCCAGC CCAACATTTC CCAGGGAAAA 
CTTCAGATGT GGGTGGATGT TTTCCCCAAG AGTTTGGGGC CACCAGGCCC TCCTTTCAAC 
ATCACACCCC GGAAAGCCAA GAAATACTAC CTGCGTGTGA TCATCTGGAA CACCAAGGAC 
GTTATCTTGG ACGAGAAAAG CATCACAGGA GAGGAAATGA GTGACATCTA CGTCAAAGGC 
TGGATTCCTG GCAATGAAGA AAACAAACAG AAAACAGATG TCCATTACAG ATCTTTGGAT 
GGTGAAGGGA ATTTTAACTG GCGATTTGTT TTCCCGTTTG ACTACCTTCC AGCCGAACAA 
CTCTGTATCG TTGCGAAAAA AGAGCATTTC TGGAGTATTG ACCAAACGGA ATTTCGAATC 
CCACCCAGGC TGATCATTCA GATATGGGAC AATGACAAGT TTTCTCTGGA TGACTACTTG 
GGTTTCCTAG AACTTGACTT GCGTCACACG ATCATTCCTG CAAAATCACC AGAGAAATGC 
AGATTGGACA TGATTCCGGA CCTCAAAGCC ATGAACCCCC TTAAAGCCAA GACAGCCTCC 
CTCTTTGAGC AGAAGTCCAT GAAAGGATGG TGGCCATGCT ACGCAGAGAA AGATGGCGCC 
CGCGTAATGG CTGGGAAAGT GGAGATGACA TTGGAAATCC TCAACGAGAA GGAGGCCGAC 
GAGAGGCCAG CCGGGAAGGG GCGGGACGAA CCCAACATGA ACCCCAAGCT GGACTTACCA 
AATCGACCAG AAACCTCCTT CCTCTGGTTC ACCAACCCAT GCAAGACCAT GAAGTTCATC 
GTGTGGCGCC GCTTTAAGTG GGTCATCATC GGCTTGCTGT TCCTGCTTAT CCTGCTGCTC 
TTCGTGGCCG TGCTCCTCTA CTCTTTGCCG AACTATTTGT CAATGAAGAT TGTAAAGCCA 
AATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537934.1
CDS	551..6739
Translation	MILPNSPPNRTDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKCLSSMSTALSKMASPATVHLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Target ORF information:

RefSeq Version	XM_011539632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoferlin (MYOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539632.2

ORF Insert Sequence:

ATGATTCTTC CAAACAGTCC TCCAAACAGA ACAGATGAGA AAAAGAAAAC AAAGAAAGTT 
GATAATGAAT TGAACCCTGT CTGGAATGAG ATTTTGGAGT TTGACTTGAG GGGTATACCA 
CTGGACTTTT CATCTTCCCT TGGGATTATT GTGAAAGATT TTGAGACAAT TGGACAAAAT 
AAATTAATTG GCACGGCGAC TGTAGCCCTG AAGGACCTGA CTGGTGACCA GAGCAGATCC 
CTGCCGTACA AGCTGATCTC CCTGCTAAAT GAAAAAGGGC AAGATACTGG GGCCACCATT 
GACTTGGTGA TCGGCTATGA TCCGCCTTCT GCTCCACATC CAAATGACCT GAGCGGGCCC 
AGCGTGCCAG GCATGGGAGG AGATGGGGAA GAAGATGAAG GTGATGAAGA CAGGTTGGAC 
AATGCAGTCA GGGGCCCTGG GCCCAAGGGG CCAGTTGGGA CGGTGTCGGA AGCTCAGCTT 
GCTCGGAGGC TCACCAAAGT AAAGAACAGC CGGCGGATGC TGTCAAATAA GCCACAGGAC 
TTCCAGATCC GCGTCCGAGT GATTGAGGGC CGACAGTTAA GTGGTAACAA CATAAGGCCT 
GTGGTCAAAG TTCACGTCTG TGGCCAGACA CACCGAACAA GAATCAAGAG AGGAAACAAC 
CCTTTTTTTG ATGAGTTGTT TTTCTACAAT GTCAACATGA CCCCTTCTGA ATTGATGGAT 
GAGATCATCA GCATCCGGGT TTATAATTCT CACTCTCTGC GGGCAGATTG TCTGATGGGG 
GAATTTAAGA TTGATGTTGG ATTTGTTTAT GATGAACCTG GCCATGCTGT CATGAGAAAG 
TGGCTTCTTC TCAATGACCC GGAAGATACC AGTTCAGGTT CTAAAGGTTA TATGAAAGTC 
AGCATGTTTG TCCTGGGAAC CGGAGATGAG CCTCCTCCTG AGAGACGAGA TCGTGATAAT 
GACAGTGATG ATGTGGAGAG TAATTTGTTA CTCCCTGCTG GCATTGCCCT CCGGTGGGTG 
ACCTTCTTGC TGAAAATCTA CCGAGCTGAG GACATCCCCC AGATGGATGA TGCCTTCTCA 
CAGACAGTAA AGGAAATATT TGGAGGCAAT GCAGATAAGA AAAATCTCGT GGATCCTTTT 
GTAGAAGTTT CCTTTGCTGG AAAAAAGGTT TGTACAAACA TAATTGAGAA AAATGCAAAC 
CCAGAGTGGA ATCAGGTCGT CAATCTTCAG ATCAAGTTTC CTTCAGTGTG TGAAAAAATA 
AAACTAACAA TATATGACTG GGACCGTCTT ACTAAAAATG ATGTAGTTGG AACAACATAT 
CTACACCTCT CTAAAATTGC TGCCTCTGGT GGGGAAGTGG AAGATTTCTC ATCTTCGGGA 
ACTGGGGCTG CATCATATAC AGTAAACACA GGAGAAACAG AGGTAGGCTT TGTTCCAACG 
TTTGGACCTT GTTACCTGAA TCTTTATGGA AGCCCCAGAG AGTACACGGG ATTCCCAGAC 
CCCTATGATG AGCTGAATAC TGGAAAGGGG GAAGGAGTTG CCTACAGAGG CAGGATCTTG 
GTTGAATTAG CCACTTTTCT TGAGAAGACA CCACCAGATA AAAAGCTTGA GCCCATTTCA 
AATGATGACC TGCTGGTTGT TGAGAAATAC CAGCGAAGGC GGAAGTACAG CCTGTCTGCC 
GTGTTTCATT CAGCCACCAT GTTGCAAGAT GTTGGTGAGG CCATTCAGTT TGAAGTCAGC 
ATTGGGAACT ATGGCAACAA GTTTGACACC ACCTGTAAGC CTTTGGCATC AACAACTCAG 
TACAGCCGTG CTGTATTTGA TGGCAACTAC TATTATTACT TGCCTTGGGC CCACACCAAG 
CCAGTTGTTA CCCTGACTTC ATACTGGGAG GATATTAGTC ATCGCCTGGA TGCGGTGAAC 
ACTCTCCTAG CTATGGCAGA ACGGCTGCAA ACAAATATAG AAGCTCTAAA ATCAGGGATA 
CAAGGTAAAA TTCCTGCAAA CCAGCTGGCT GAATTGTGGC TGAAGCTGAT AGATGAAGTT 
ATAGAAGACA CGAGATACAC GTTGCCTCTC ACAGAAGGAA AAGCCAACGT CACAGTTCTC 
GATACTCAGA TCCGAAAGCT GCGGTCCAGG TCTCTCTCCC AAATACATGA GGCGGCTGTG 
AGGATGAGGT CGGAAGCCAC AGATGTGAAG TCCACACTGG CAGAAATTGA GGACTGGCTT 
GATAAATTAA TGCAGCTGAC TGAAGAGCCA CAGAACAGCA TGCCTGACAT CATCATCTGG 
ATGATCCGGG GAGAGAAGAG ACTGGCCTAT GCACGAATTC CCGCACATCA GGTCTTGTAC 
TCCACCAGTG GTGAGAATGC ATCTGGAAAA TACTGTGGGA AAACCCAAAC CATCTTTCTG 
AAGTATCCAC AGGAGAAAAA CAACGGGCCA AAGGTGCCTG TGGAGTTGCG AGTGAACATC 
TGGCTAGGCT TAAGTGCTGT GGAGAAGAAG TTTAACAGCT TCGCAGAAGG AACTTTCACC 
GTCTTTGCTG AAATGTATGA AAATCAAGCT CTCATGTTTG GAAAATGGGG TACTTCTGGA 
TTAGTAGGAC GTCATAAGTT TTCTGATGTC ACAGGAAAAA TAAAACTCAA GAGGGAATTT 
TTTCTGCCTC CAAAAGGCTG GGAATGGGAA GGAGAGTGGA TAGTTGATCC TGAAAGAAGC 
TTGCTGACTG AGGCAGATGC AGGTCACACG GAGTTCACTG ATGAAGTCTA TCAGAACGAG 
AGCCGCTACC CCGGGGGCGA CTGGAAGCCG GCCGAGGACA CCTACACGGA TGCGAACGGC 
GATAAAGCAG CATCACCCAG CGAGTTGACT TGTCCTCCAG GTTGGGAATG GGAAGATGAT 
GCATGGTCTT ATGACATAAA TCGAGCGGTG GATGAGAAAG GCTGGGAATA TGGAATCACC 
ATTCCTCCTG ATCATAAGCC CAAATCCTGG GTTGCAGCAG AGAAAATGTA CCACACTCAT 
AGACGGCGAA GGCTGGTCCG AAAACGCAAG AAAGATTTAA CACAGACTGC TTCAAGCACC 
GCAAGGGCCA TGGAGGAATT GCAAGACCAA GAGGGCTGGG AATATGCTTC TCTAATTGGC 
TGGAAATTTC ACTGGAAACA ACGTAGTTCA GATACCTTCC GCCGCAGACG CTGGAGGAGA 
AAAATGGCTC CTTCAGAAAC ACATGGTGCA GCTGCCATCT TTAAACTTGA AGGTGCCCTT 
GGGGCAGACA CTACCGAAGA TGGGGATGAG AAGAGCCTGG AGAAACAGAA GCACAGTGCC 
ACCACTGTGT TCGGAGCAAA CACCCCCATT GTTTCCTGCA ATTTTGACAG AGTCTACATC 
TACCATCTGC GCTGCTATGT CTATCAAGCC AGAAACCTCT TGGCTTTAGA TAAGGATAGC 
TTTTCAGATC CATATGCTCA TATCTGTTTC CTCCATCGGA GCAAAACCAC TGAGATCATC 
CATTCAACCC TGAATCCCAC GTGGGACCAA ACAATTATAT TCGATGAAGT TGAAATCTAT 
GGGGAACCCC AAACAGTTCT ACAGAATCCA CCCAAAGTTA TCATGGAACT TTTTGACAAT 
GACCAAGTGG GCAAAGATGA ATTTTTAGGA CGAAGCATTT TCTCTCCTGT GGTGAAACTG 
AACTCAGAAA TGGACATCAC ACCCAAACTT CTCTGGCACC CAGTAATGAA TGGAGACAAA 
GCCTGCGGGG ATGTTCTTGT AACTGCAGAG CTGATTCTGA GGGGCAAGGA TGGCTCCAAC 
CTTCCCATTC TTCCCCCTCA AAGGGCGCCA AATCTATACA TGGTCCCCCA GGGGATCAGG 
CCTGTGGTCC AGCTCACTGC CATTGAGATT CTAGCTTGGG GCTTAAGAAA TATGAAAAAC 
TTCCAGATGG CTTCTATCAC ATCCCCCAGT CTTGTTGTGG AGTGTGGAGG AGAAAGGGTG 
GAATCGGTGG TGATCAAAAA CCTTAAGAAG ACACCCAACT TTCCAAGTTC TGTTCTCTTC 
ATGAAAGTGT TCTTGCCCAA GGAGGAATTG TACATGCCCC CACTGGTGAT CAAGGTCATC 
GACCACAGGC AGTTTGGGCG GAAGCCTGTC GTCGGCCAGT GCACCATCGA GCGCCTGGAC 
CGCTTTCGCT GTGACCCTTA TGCAGGGAAA GAGGACATCG TCCCACAGCT CAAAGCCTCC 
CTTCTGTCTG CCCCACCATG CCGGGACATC GTTATCGAAA TGGAAGACAC CAAACCATTA 
CTGGCTTCTA AGTGCTTAAG CAGTATGTCA ACAGCACTCA GCAAAATGGC TTCTCCAGCG 
ACAGTGCATC TGACAGAAAA GGAGGAAGAA ATCGTGGACT GGTGGAGTAA ATTTTATGCT 
TCCTCAGGGG AACATGAAAA ATGCGGACAG TATATTCAGA AAGGCTATTC CAAGCTCAAG 
ATATATAATT GTGAACTAGA AAATGTAGCA GAATTTGAGG GCCTGACAGA CTTCTCAGAT 
ACGTTCAAGT TGTACCGAGG CAAGTCGGAT GAAAATGAAG ATCCTTCTGT GGTTGGAGAG 
TTTAAGGGCT CCTTTCGGAT CTACCCTCTG CCGGATGACC CCAGCGTGCC AGCCCCTCCC 
AGACAGTTTC GGGAATTACC TGACAGCGTC CCACAGGAAT GCACGGTTAG GATTTACATT 
GTTCGAGGCT TAGAGCTCCA GCCCCAGGAC AACAATGGCC TGTGTGACCC TTACATAAAA 
ATAACACTGG GCAAAAAAGT CATTGAAGAC CGAGATCACT ACATTCCCAA CACTCTCAAC 
CCAGTCTTTG GCAGGATGTA CGAACTGAGC TGCTACTTAC CTCAAGAAAA AGACCTGAAA 
ATTTCTGTCT ATGATTATGA CACCTTTACC CGGGATGAAA AAGTAGGAGA AACAATTATT 
GATCTGGAAA ACCGATTCCT TTCCCGCTTT GGGTCCCACT GCGGCATACC AGAGGAGTAC 
TGTGTTTCTG GAGTCAATAC CTGGCGAGAT CAACTGAGAC CAACACAGCT GCTTCAAAAT 
GTCGCCAGAT TCAAAGGCTT CCCACAACCC ATCCTTTCCG AAGATGGGAG TAGAATCAGA 
TATGGAGGAC GAGACTACAG CTTGGATGAA TTTGAAGCCA ACAAAATCCT GCACCAGCAC 
CTCGGGGCCC CTGAAGAGCG GCTTGCTCTT CACATCCTCA GGACTCAGGG GCTGGTCCCT 
GAGCACGTGG AAACAAGGAC TTTGCACAGC ACCTTCCAGC CCAACATTTC CCAGGGAAAA 
CTTCAGATGT GGGTGGATGT TTTCCCCAAG AGTTTGGGGC CACCAGGCCC TCCTTTCAAC 
ATCACACCCC GGAAAGCCAA GAAATACTAC CTGCGTGTGA TCATCTGGAA CACCAAGGAC 
GTTATCTTGG ACGAGAAAAG CATCACAGGA GAGGAAATGA GTGACATCTA CGTCAAAGGC 
TGGATTCCTG GCAATGAAGA AAACAAACAG AAAACAGATG TCCATTACAG ATCTTTGGAT 
GGTGAAGGGA ATTTTAACTG GCGATTTGTT TTCCCGTTTG ACTACCTTCC AGCCGAACAA 
CTCTGTATCG TTGCGAAAAA AGAGCATTTC TGGAGTATTG ACCAAACGGA ATTTCGAATC 
CCACCCAGGC TGATCATTCA GATATGGGAC AATGACAAGT TTTCTCTGGA TGACTACTTG 
GGTTTCCTAG AACTTGACTT GCGTCACACG ATCATTCCTG CAAAATCACC AGAGAAATGC 
AGATTGGACA TGATTCCGGA CCTCAAAGCC ATGAACCCCC TTAAAGCCAA GACAGCCTCC 
CTCTTTGAGC AGAAGTCCAT GAAAGGATGG TGGCCATGCT ACGCAGAGAA AGATGGCGCC 
CGCGTAATGG CTGGGAAAGT GGAGATGACA TTGGAAATCC TCAACGAGAA GGAGGCCGAC 
GAGAGGCCAG CCGGGAAGGG GCGGGACGAA CCCAACATGA ACCCCAAGCT GGACTTACCA 
AATCGACCAG AAACCTCCTT CCTCTGGTTC ACCAACCCAT GCAAGACCAT GAAGTTCATC 
GTGTGGCGCC GCTTTAAGTG GGTCATCATC GGCTTGCTGT TCCTGCTTAT CCTGCTGCTC 
TTCGTGGCCG TGCTCCTCTA CTCTTTGCCG AACTATTTGT CAATGAAGAT TGTAAAGCCA 
AATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYOF
Entrez Gene ID	26509
Full Name	myoferlin
Synonyms	FER1L3
General protein information	Preferred Names myoferlin Names myoferlin fer-1-like 3, myoferlin fer-1-like family member 3 fer-1-like protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.33
Summary	Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008].
Disorder MIM:	604603

Danio rerio (zebrafish)	myof	XP_687465.5
	MYOF	XP_001089235.2
Gallus gallus (chicken)	MYOF	XP_001231546.2
Homo sapiens (human)	MYOF	NP_038479.1
Pan troglodytes (chimpanzee)	MYOF	XP_003312803.1
Mus musculus (house mouse)	Myof	NP_001093104.1
Xenopus tropicalis (tropical clawed frog)	myof	NP_001122123.1
Bos taurus (cattle)	MYOF	NP_001180141.1
Rattus norvegicus (Norway rat)	Myof	NP_001178565.1
Canis lupus familiaris (dog)	MYOF	XP_858858.2

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MYOF cDNA ORF clone, Homo sapiens(human)

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gRNAs

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