Myof cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myof
Entrez Gene ID	226101
Full Name	myoferlin
Synonyms	2310004N10Rik,2310051D19Rik,E030042N20Rik,Fer1l3
General protein information	Preferred Names myoferlin Names myoferlin fer-1-like protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C2
Summary	The protein encoded by this gene is a member of the ferlin family of proteins, which have been implicated in fusion events in muscle tissue. Members of this family have a carboxy-terminal single pass transmembrane domain and multiple C2 domains, which bind negatively charged phospholipids in the presence of calcium ions. This gene is expressed at high levels in myoblasts and upregulated in damaged skeletal muscle. Mice deficient in this protein display defects in myoblast fusion, muscle regeneration, and angiogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526995.1	XP_006527058.1	myoferlin isoform X2
NM_001302140.1	NP_001289069.1	myoferlin isoform 1
XM_006526994.1	XP_006527057.1	myoferlin isoform X1
NM_001099634.1	NP_001093104.1	myoferlin isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	myof	XP_687465.5
	MYOF	XP_001089235.2
Gallus gallus (chicken)	MYOF	XP_001231546.2
Homo sapiens (human)	MYOF	NP_038479.1
Pan troglodytes (chimpanzee)	MYOF	XP_003312803.1
Mus musculus (house mouse)	Myof	NP_001093104.1
Xenopus tropicalis (tropical clawed frog)	myof	NP_001122123.1
Bos taurus (cattle)	MYOF	NP_001180141.1
Rattus norvegicus (Norway rat)	Myof	NP_001178565.1
Canis lupus familiaris (dog)	MYOF	XP_858858.2

Gene Ontology
Bibliography

Related articles in PubMed

Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.
Bhattacharyya S, Rainey MA, Arya P, Dutta S, George M, Storck MD, McComb RD, Muirhead D, Todd GL, Gould K, Datta K, Gelineau-van Waes J, Band V, Band H
Scientific reports620727(2016 Feb)

Myoferlin regulation by NFAT in muscle injury, regeneration and repair.
Demonbreun AR, Lapidos KA, Heretis K, Levin S, Dale R, Pytel P, Svensson EC, McNally EM
Journal of cell science123(Pt 14)2413-22(2010 Jul)

Myoferlin is required for insulin-like growth factor response and muscle growth.
Demonbreun AR, Posey AD, Heretis K, Swaggart KA, Earley JU, Pytel P, McNally EM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(4)1284-95(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings demonstrate the importance of dysferlin and myoferlin for transverse tubule function and in the genesis of muscular dystrophy.
Title: Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.

These data provide the first evidence of myoferlin expression in solid human and mouse tumors.
Title: Expression of myoferlin in human and murine carcinoma tumors: role in membrane repair, cell proliferation, and tumorigenesis.

Myoferlin gene knockdown not only decreases lipid vesicle fusion in EC but also attenuates Vascular Endothelial Growth Factor (VEGF) Receptor-2 (VEGFR-2) expression.
Title: Myoferlin gene silencing decreases Tie-2 expression in vitro and angiogenesis in vivo.

increased expression in myofibres after muscle injury and in a mouse model of muscular dystrophy
Title: Myoferlin regulation by NFAT in muscle injury, regeneration and repair.

IGF1 receptor recycling is required for normal myogenesis and myoferlin is a critical mediator of postnatal muscle growth mediated by IGF1
Title: Myoferlin is required for insulin-like growth factor response and muscle growth.

The following Myof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15885	NM_001099634.1 Latest version!	Mus musculus myoferlin (Myof), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu73275	NM_001302140.1 Latest version!	Mus musculus myoferlin (Myof), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu55485	XM_006526994.1 Latest version!	Mus musculus myoferlin (Myof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu55486	XM_006526995.1 Latest version!	Mus musculus myoferlin (Myof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myof ( NM_001099634.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001093104.1 Mus musculus myoferlin (Myof), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15885
Clone ID Related Accession (Same CDS sequence)	NM_001099634.1
Accession Version	NM_001099634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	myoferlin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC115360.6. On Jul 25, 2007 this sequence version replaced XM_283556.7. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA CTACAGGAGA GACAGAGGTG 
GGGTTTGTCC CAACATTCGG ACCTTGTTAT TTGAACCTTT ACGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA TGATGAGCTC AACTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTCGTGGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTGGAGCCCA TTTCCAGTGA TGACCTTCTG GTTGTTGAGA AGTACCAGCG AAGGCGGAAG 
TATAGCCTGT CCGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTTTG 
GCATCAACCA CTCAATACAG CCGTGCAGTA TTTGACGGGA ACTACTATTA CTACTTACCT 
TGGGCCCACA CGAAGCCCGT GGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCACCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATAGAGGCT 
GTAAAATCAG GGATCCAGGG TAAAATTCCT GCAAACCAGT TGGCGGAGGT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACCTTGC CTGTCACAGA AGGAAAAGCC 
AACGTCACGG TTCTGGATAC TCAGATCCGA AAGTTGCGGT CCAGGTTTCT CTCCCAGATA 
CACGAGGCAG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGTTGGAA 
ATCGAGGAGT GGCTTGATAA ACTGATGCAG CTTACTGAAG AGCCTCAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGTGGAGAG AAAAGACTGG CTTATGCACG AATCCCTGCC 
CACCAAGTCT TGTACTCCAC CAGCGGTGGG AACGCCTCTG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAAACCAATG GGCCCAAAGT GCCTGTGGAG 
CTGCGCGTGA ACATCTGGCT GGGTTTAAGT GCTGTGGAAA AGAAGTTCAA TAGCTTCGCG 
GAAGGCACCT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTTGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAGT GGGAAGGAGA CTGGGTGGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GACGCAGGTC ACACCGAGTT CACTGATGAA 
GTCTACCAGA ATGAGAATCG CTACCCCGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACGGATGCGA ATGGAGATAA GGCGGCATCA CCCAGTGAGA TGACCTGCCC TCCAGGTTGG 
GAGTGGGAGG ATGACGCATG GATATATGAC ATAAATCGAG CAGTGGATGA GAAAGGCTGG 
GAGTATGGGA TTACCATTCC TCCTGATAAT AAGCCCAAAT CCTGGGTTGC AGCAGAAAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGGAAAC GCAAGAAAGA TCTGACACAG 
ACGGCTTCTA GTACCGCAAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAT 
GCTTCGTTAA TCGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGACAC CTTTCGCCGC 
AGGCGCTGGA GGAGGAAAAT GGCCCCTTCT GAGACACACG GTGCCGCTGC CATCTTTAAA 
CTCGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG AAGAGAAGGG CCCGGAGAAG 
CAGAAGCACA GCGCCACCAC TGTGTTTGGA GCAAACACGC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTGCGCTGC TACATTTATC AAGCCAGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGATCCATAC GCCCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATCCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTCGAT 
GAGGTTGAAA TCTTTGGGGA ACCCCAAACA GTTCTACAGA ACCCACCCAA CGTTACCATA 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGTCGAAG CATTTGCTCT 
CCCTTGGTGA AACTGAACTC AGAAACGGAC ATCACACCTA AGCTTCTCTG GCATCCTGTT 
ATGAATGGAG ACAAGGCCTG TGGGGATGTC CTCGTAACAG CAGAGTTGAT TCTGAGGAAC 
AAGGATGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAGGGGA TCAGGCCTGT GGTTCAGCTC ACTGCCATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCT GTCACATCAC CCAGCCTGGT GGTGGAATGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTTA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCTCCGCTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTT GGGCGGAAGC CTGTGGTTGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA TATTGTACCA 
CAACTAAAAG CCTCTCTGAT GTCTGCACCT CCATGCCGGG AAGTCGTTAT TGAAATAGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG TCGGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCTGGAGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ATGTAGCCGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGCTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCATCTGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCAG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC ACAGGAATGC 
ACTGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CTCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCTTGGGC AAGAAAGTAA TTGAAGACCG AGACCACTAC 
ATTCCCAACA CTCTCAACCC AGTGTTTGGC AGAATGTACG AACTGAGCTG CTACTTGCCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGATA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTTT CCCGGTTCGG GTCTCACTGC 
GGAATCCCTG AGCAGTACTG TGTTTCCGGA GTAAATACCT GGCGAGATCA GCTGAGACCA 
ACGCAGTTGC TTCAAAACGT TGCAAGATTC AAAGGCTTCC CGCCACCAGT TCTTTCTGAA 
GACGGAAGCA GAATCCGATA CGGAGGTCGA GACTACCATT TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCC GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TTTAGGACCA 
CCAGGCCCCC CTTTCAACAT CACACCCAGG AAAGCCAAGA AGTACTACCT GCGTGTGATC 
ATCTGGAATA CCAAGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGT 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG TGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCTG CTGAGCAGCT CTGTATAGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TTCGAGTCCC TCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTTGACTTAC ATCGTACGAT CATCCCAGCC 
AAGACATCAG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAAGCTAAGA CTGCCTCACT CTTTGAACAG AGGTCCATGA AAGGATGGTG GCCGTGCTAC 
GCAGACAAAG ATGGCACCCG GGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTGCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGTTGCTGTT CGTGGCTGTG CTCCTGTACT CTCTTCCGAA CTATTTATCA 
ATGAAGATTG TGAGGCCGAA TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093104.1
CDS	255..6401
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	NM_001099634.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myoferlin (Myof), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099634.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA CTACAGGAGA GACAGAGGTG 
GGGTTTGTCC CAACATTCGG ACCTTGTTAT TTGAACCTTT ACGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA TGATGAGCTC AACTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTCGTGGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTGGAGCCCA TTTCCAGTGA TGACCTTCTG GTTGTTGAGA AGTACCAGCG AAGGCGGAAG 
TATAGCCTGT CCGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTTTG 
GCATCAACCA CTCAATACAG CCGTGCAGTA TTTGACGGGA ACTACTATTA CTACTTACCT 
TGGGCCCACA CGAAGCCCGT GGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCACCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATAGAGGCT 
GTAAAATCAG GGATCCAGGG TAAAATTCCT GCAAACCAGT TGGCGGAGGT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACCTTGC CTGTCACAGA AGGAAAAGCC 
AACGTCACGG TTCTGGATAC TCAGATCCGA AAGTTGCGGT CCAGGTTTCT CTCCCAGATA 
CACGAGGCAG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGTTGGAA 
ATCGAGGAGT GGCTTGATAA ACTGATGCAG CTTACTGAAG AGCCTCAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGTGGAGAG AAAAGACTGG CTTATGCACG AATCCCTGCC 
CACCAAGTCT TGTACTCCAC CAGCGGTGGG AACGCCTCTG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAAACCAATG GGCCCAAAGT GCCTGTGGAG 
CTGCGCGTGA ACATCTGGCT GGGTTTAAGT GCTGTGGAAA AGAAGTTCAA TAGCTTCGCG 
GAAGGCACCT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTTGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAGT GGGAAGGAGA CTGGGTGGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GACGCAGGTC ACACCGAGTT CACTGATGAA 
GTCTACCAGA ATGAGAATCG CTACCCCGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACGGATGCGA ATGGAGATAA GGCGGCATCA CCCAGTGAGA TGACCTGCCC TCCAGGTTGG 
GAGTGGGAGG ATGACGCATG GATATATGAC ATAAATCGAG CAGTGGATGA GAAAGGCTGG 
GAGTATGGGA TTACCATTCC TCCTGATAAT AAGCCCAAAT CCTGGGTTGC AGCAGAAAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGGAAAC GCAAGAAAGA TCTGACACAG 
ACGGCTTCTA GTACCGCAAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAT 
GCTTCGTTAA TCGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGACAC CTTTCGCCGC 
AGGCGCTGGA GGAGGAAAAT GGCCCCTTCT GAGACACACG GTGCCGCTGC CATCTTTAAA 
CTCGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG AAGAGAAGGG CCCGGAGAAG 
CAGAAGCACA GCGCCACCAC TGTGTTTGGA GCAAACACGC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTGCGCTGC TACATTTATC AAGCCAGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGATCCATAC GCCCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATCCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTCGAT 
GAGGTTGAAA TCTTTGGGGA ACCCCAAACA GTTCTACAGA ACCCACCCAA CGTTACCATA 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGTCGAAG CATTTGCTCT 
CCCTTGGTGA AACTGAACTC AGAAACGGAC ATCACACCTA AGCTTCTCTG GCATCCTGTT 
ATGAATGGAG ACAAGGCCTG TGGGGATGTC CTCGTAACAG CAGAGTTGAT TCTGAGGAAC 
AAGGATGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAGGGGA TCAGGCCTGT GGTTCAGCTC ACTGCCATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCT GTCACATCAC CCAGCCTGGT GGTGGAATGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTTA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCTCCGCTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTT GGGCGGAAGC CTGTGGTTGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA TATTGTACCA 
CAACTAAAAG CCTCTCTGAT GTCTGCACCT CCATGCCGGG AAGTCGTTAT TGAAATAGAA 
GACACCAAAC CATTACTGGC TTCTAAGCTG TCGGAAAAGG AGGAAGAAAT CGTTGACTGG 
TGGAGTAAAT TTTATGCTTC CTCTGGAGAG CATGAAAAAT GTGGACAGTA TATTCAGAAA 
GGCTATTCCA AACTCAAGAT CTATGACTGT GAACTGGAGG ATGTAGCCGA CTTTGAAGGC 
CTGACAGATT TCTCAGATAC CTTCAAGCTG TACAGAGGCA AGTCCGATGA GAATGAAGAT 
CCATCTGTGG TAGGAGAGTT TAAAGGCTCC TTCCGAATCT ACCCCTTGCC GGATGACCCC 
AGTGTGCCAG CCCCTCCCAG GCAGTTTCGG GAACTGCCTG ACAGTGTCCC ACAGGAATGC 
ACTGTTAGGA TTTACATTGT TCAAGGCTTA CAGCTCCAGC CTCAGGACAA CAATGGCCTG 
TGTGATCCTT ACATAAAAAT AACCTTGGGC AAGAAAGTAA TTGAAGACCG AGACCACTAC 
ATTCCCAACA CTCTCAACCC AGTGTTTGGC AGAATGTACG AACTGAGCTG CTACTTGCCT 
CAAGAGAAAG ACCTGAAGAT CTCTGTCTAT GACTACGATA CCTTCACCCG AGATGAGAAG 
GTGGGAGAGA CAACCATTGA CCTGGAGAAC CGCTTCCTTT CCCGGTTCGG GTCTCACTGC 
GGAATCCCTG AGCAGTACTG TGTTTCCGGA GTAAATACCT GGCGAGATCA GCTGAGACCA 
ACGCAGTTGC TTCAAAACGT TGCAAGATTC AAAGGCTTCC CGCCACCAGT TCTTTCTGAA 
GACGGAAGCA GAATCCGATA CGGAGGTCGA GACTACCATT TGGATGAGTT TGAGGCCAAC 
AAAATCCTGC ACCAGCACCT TGGAGCCCCC GAAGAGCGTC TTGCCCTCCA TATTCTCAGG 
ACTCAGGGGC TGGTCCCTGA GCACGTGGAG ACAAGGACTT TGCACAGCAC CTTCCAGCCC 
AATATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTCT TCCCCAAGAG TTTAGGACCA 
CCAGGCCCCC CTTTCAACAT CACACCCAGG AAAGCCAAGA AGTACTACCT GCGTGTGATC 
ATCTGGAATA CCAAGGACGT TATCTTGGAT GAGAAGAGCA TCACTGGGGA GGACATGAGT 
GACATCTACG TCAAAGGCTG GATTTCCGGC AGCGAAGAAA ACAAACAGAA AACAGATGTC 
CACTACAGGT CCTTGGATGG TGAAGGCAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC 
TACCTCCCTG CTGAGCAGCT CTGTATAGTC GCTAAAAAAG AGCATTTCTG GAGTATTGAT 
CAAACAGAAT TTCGAGTCCC TCCTCGGCTG ATCATTCAGA TATGGGACAA TGACAAGTTT 
TCCCTGGACG ACTACTTGGG TTTTCTGGAA CTTGACTTAC ATCGTACGAT CATCCCAGCC 
AAGACATCAG AGAAATGCAG TTTGGACATG ATTCCAGACC TCAAAGCCAT GGACCCTCTT 
AAAGCTAAGA CTGCCTCACT CTTTGAACAG AGGTCCATGA AAGGATGGTG GCCGTGCTAC 
GCAGACAAAG ATGGCACCCG GGTGATGGCT GGGAAAGTGG AGATGACACT GGAGGTGCTC 
AACGAGAGGG AGGCCGACGA GAGGCCAGCC GGGAAGGGGC GGAGTGAACC CAACATGAAC 
CCCAAGCTTG ACCCACCAAA CCGACCGGAA ACCTCCTTCC TCTGGTTCAC GAACCCTTGC 
AAGACCATGC GCTTCATCGT GTGGCGCCGG TTCAAGTGGG TGATCATCGG CCTGCTTCTG 
CTGCTCATCC TGTTGCTGTT CGTGGCTGTG CTCCTGTACT CTCTTCCGAA CTATTTATCA 
ATGAAGATTG TGAGGCCGAA TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( NM_001302140.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289069.1 Mus musculus myoferlin (Myof), transcript variant 1, mRNA.

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CloneID	OMu73275
Clone ID Related Accession (Same CDS sequence)	NM_001302140.1
Accession Version	NM_001302140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	myoferlin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY743035.1, AK086107.1, BQ551773.1, BB612787.1, CN701681.1, AK075903.1 and AK028612.1. On Oct 3, 2014 this sequence version replaced XM_006526996.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAGCTGGG 
GCTGCATCAT ATACAGCAAC TACAGGAGAG ACAGAGGTGG GGTTTGTCCC AACATTCGGA 
CCTTGTTATT TGAACCTTTA CGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAT 
GATGAGCTCA ACTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTCGTGGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TGGAGCCCAT TTCCAGTGAT 
GACCTTCTGG TTGTTGAGAA GTACCAGCGA AGGCGGAAGT ATAGCCTGTC CGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTTTGG CATCAACCAC TCAATACAGC 
CGTGCAGTAT TTGACGGGAA CTACTATTAC TACTTACCTT GGGCCCACAC GAAGCCCGTG 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCACCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATAGAGGCTG TAAAATCAGG GATCCAGGGT 
AAAATTCCTG CAAACCAGTT GGCGGAGGTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACCTTGCC TGTCACAGAA GGAAAAGCCA ACGTCACGGT TCTGGATACT 
CAGATCCGAA AGTTGCGGTC CAGGTTTCTC TCCCAGATAC ACGAGGCAGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGTTGGAAA TCGAGGAGTG GCTTGATAAA 
CTGATGCAGC TTACTGAAGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGTGGAGAGA AAAGACTGGC TTATGCACGA ATCCCTGCCC ACCAAGTCTT GTACTCCACC 
AGCGGTGGGA ACGCCTCTGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AAACCAATGG GCCCAAAGTG CCTGTGGAGC TGCGCGTGAA CATCTGGCTG 
GGTTTAAGTG CTGTGGAAAA GAAGTTCAAT AGCTTCGCGG AAGGCACCTT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTTGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAGTG GGAAGGAGAC TGGGTGGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ACGCAGGTCA CACCGAGTTC ACTGATGAAG TCTACCAGAA TGAGAATCGC 
TACCCCGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CGGATGCGAA TGGAGATAAG 
GCGGCATCAC CCAGTGAGAT GACCTGCCCT CCAGGTTGGG AGTGGGAGGA TGACGCATGG 
ATATATGACA TAAATCGAGC AGTGGATGAG AAAGGCTGGG AGTATGGGAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGGAAACG CAAGAAAGAT CTGACACAGA CGGCTTCTAG TACCGCAAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATATG CTTCGTTAAT CGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGACACC TTTCGCCGCA GGCGCTGGAG GAGGAAAATG 
GCCCCTTCTG AGACACACGG TGCCGCTGCC ATCTTTAAAC TCGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA AGAGAAGGGC CCGGAGAAGC AGAAGCACAG CGCCACCACT 
GTGTTTGGAG CAAACACGCC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTGCGCTGCT ACATTTATCA AGCCAGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GATCCATACG CCCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTCGATG AGGTTGAAAT CTTTGGGGAA 
CCCCAAACAG TTCTACAGAA CCCACCCAAC GTTACCATAG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGTCGAAGC ATTTGCTCTC CCTTGGTGAA ACTGAACTCA 
GAAACGGACA TCACACCTAA GCTTCTCTGG CATCCTGTTA TGAATGGAGA CAAGGCCTGT 
GGGGATGTCC TCGTAACAGC AGAGTTGATT CTGAGGAACA AGGATGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAGGGGAT CAGGCCTGTG 
GTTCAGCTCA CTGCCATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCTG TCACATCACC CAGCCTGGTG GTGGAATGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTTAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCTCCGCTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTTG GGCGGAAGCC TGTGGTTGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAT ATTGTACCAC AACTAAAAGC CTCTCTGATG 
TCTGCACCTC CATGCCGGGA AGTCGTTATT GAAATAGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGT CGGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCTGGAGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA TGTAGCCGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGCTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CATCTGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCAGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCA CAGGAATGCA CTGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC TCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCTTGGGCA AGAAAGTAAT TGAAGACCGA GACCACTACA TTCCCAACAC TCTCAACCCA 
GTGTTTGGCA GAATGTACGA ACTGAGCTGC TACTTGCCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGATAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTTTC CCGGTTCGGG TCTCACTGCG GAATCCCTGA GCAGTACTGT 
GTTTCCGGAG TAAATACCTG GCGAGATCAG CTGAGACCAA CGCAGTTGCT TCAAAACGTT 
GCAAGATTCA AAGGCTTCCC GCCACCAGTT CTTTCTGAAG ACGGAAGCAG AATCCGATAC 
GGAGGTCGAG ACTACCATTT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCCG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GGGGAAACTT 
CAGATGTGGG TGGATGTCTT CCCCAAGAGT TTAGGACCAC CAGGCCCCCC TTTCAACATC 
ACACCCAGGA AAGCCAAGAA GTACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT 
ATCTTGGATG AGAAGAGCAT CACTGGGGAG GACATGAGTG ACATCTACGT CAAAGGCTGG 
ATTTCCGGCA GCGAAGAAAA CAAACAGAAA ACAGATGTCC ACTACAGGTC CTTGGATGGT 
GAAGGCAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCTGC TGAGCAGCTC 
TGTATAGTCG CTAAAAAAGA GCATTTCTGG AGTATTGATC AAACAGAATT TCGAGTCCCT 
CCTCGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CCCTGGACGA CTACTTGGGT 
TTTCTGGAAC TTGACTTACA TCGTACGATC ATCCCAGCCA AGACATCAGA GAAATGCAGT 
TTGGACATGA TTCCAGACCT CAAAGCCATG GACCCTCTTA AAGCTAAGAC TGCCTCACTC 
TTTGAACAGA GGTCCATGAA AGGATGGTGG CCGTGCTACG CAGACAAAGA TGGCACCCGG 
GTGATGGCTG GGAAAGTGGA GATGACACTG GAGGTGCTCA ACGAGAGGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GAGTGAACCC AACATGAACC CCAAGCTTGA CCCACCAAAC 
CGACCGGAAA CCTCCTTCCT CTGGTTCACG AACCCTTGCA AGACCATGCG CTTCATCGTG 
TGGCGCCGGT TCAAGTGGGT GATCATCGGC CTGCTTCTGC TGCTCATCCT GTTGCTGTTC 
GTGGCTGTGC TCCTGTACTC TCTTCCGAAC TATTTATCAA TGAAGATTGT GAGGCCGAAT 
GCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289069.1
CDS	255..6440
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDFSSSGAGAASYTATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	NM_001302140.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myoferlin (Myof), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302140.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAGCTGGG 
GCTGCATCAT ATACAGCAAC TACAGGAGAG ACAGAGGTGG GGTTTGTCCC AACATTCGGA 
CCTTGTTATT TGAACCTTTA CGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAT 
GATGAGCTCA ACTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTCGTGGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TGGAGCCCAT TTCCAGTGAT 
GACCTTCTGG TTGTTGAGAA GTACCAGCGA AGGCGGAAGT ATAGCCTGTC CGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTTTGG CATCAACCAC TCAATACAGC 
CGTGCAGTAT TTGACGGGAA CTACTATTAC TACTTACCTT GGGCCCACAC GAAGCCCGTG 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCACCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATAGAGGCTG TAAAATCAGG GATCCAGGGT 
AAAATTCCTG CAAACCAGTT GGCGGAGGTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACCTTGCC TGTCACAGAA GGAAAAGCCA ACGTCACGGT TCTGGATACT 
CAGATCCGAA AGTTGCGGTC CAGGTTTCTC TCCCAGATAC ACGAGGCAGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGTTGGAAA TCGAGGAGTG GCTTGATAAA 
CTGATGCAGC TTACTGAAGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGTGGAGAGA AAAGACTGGC TTATGCACGA ATCCCTGCCC ACCAAGTCTT GTACTCCACC 
AGCGGTGGGA ACGCCTCTGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AAACCAATGG GCCCAAAGTG CCTGTGGAGC TGCGCGTGAA CATCTGGCTG 
GGTTTAAGTG CTGTGGAAAA GAAGTTCAAT AGCTTCGCGG AAGGCACCTT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTTGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAGTG GGAAGGAGAC TGGGTGGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ACGCAGGTCA CACCGAGTTC ACTGATGAAG TCTACCAGAA TGAGAATCGC 
TACCCCGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CGGATGCGAA TGGAGATAAG 
GCGGCATCAC CCAGTGAGAT GACCTGCCCT CCAGGTTGGG AGTGGGAGGA TGACGCATGG 
ATATATGACA TAAATCGAGC AGTGGATGAG AAAGGCTGGG AGTATGGGAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGGAAACG CAAGAAAGAT CTGACACAGA CGGCTTCTAG TACCGCAAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATATG CTTCGTTAAT CGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGACACC TTTCGCCGCA GGCGCTGGAG GAGGAAAATG 
GCCCCTTCTG AGACACACGG TGCCGCTGCC ATCTTTAAAC TCGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA AGAGAAGGGC CCGGAGAAGC AGAAGCACAG CGCCACCACT 
GTGTTTGGAG CAAACACGCC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTGCGCTGCT ACATTTATCA AGCCAGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GATCCATACG CCCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTCGATG AGGTTGAAAT CTTTGGGGAA 
CCCCAAACAG TTCTACAGAA CCCACCCAAC GTTACCATAG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGTCGAAGC ATTTGCTCTC CCTTGGTGAA ACTGAACTCA 
GAAACGGACA TCACACCTAA GCTTCTCTGG CATCCTGTTA TGAATGGAGA CAAGGCCTGT 
GGGGATGTCC TCGTAACAGC AGAGTTGATT CTGAGGAACA AGGATGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAGGGGAT CAGGCCTGTG 
GTTCAGCTCA CTGCCATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCTG TCACATCACC CAGCCTGGTG GTGGAATGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTTAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCTCCGCTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTTG GGCGGAAGCC TGTGGTTGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAT ATTGTACCAC AACTAAAAGC CTCTCTGATG 
TCTGCACCTC CATGCCGGGA AGTCGTTATT GAAATAGAAG ACACCAAACC ATTACTGGCT 
TCTAAGCTGT CGGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC 
TCTGGAGAGC ATGAAAAATG TGGACAGTAT ATTCAGAAAG GCTATTCCAA ACTCAAGATC 
TATGACTGTG AACTGGAGGA TGTAGCCGAC TTTGAAGGCC TGACAGATTT CTCAGATACC 
TTCAAGCTGT ACAGAGGCAA GTCCGATGAG AATGAAGATC CATCTGTGGT AGGAGAGTTT 
AAAGGCTCCT TCCGAATCTA CCCCTTGCCG GATGACCCCA GTGTGCCAGC CCCTCCCAGG 
CAGTTTCGGG AACTGCCTGA CAGTGTCCCA CAGGAATGCA CTGTTAGGAT TTACATTGTT 
CAAGGCTTAC AGCTCCAGCC TCAGGACAAC AATGGCCTGT GTGATCCTTA CATAAAAATA 
ACCTTGGGCA AGAAAGTAAT TGAAGACCGA GACCACTACA TTCCCAACAC TCTCAACCCA 
GTGTTTGGCA GAATGTACGA ACTGAGCTGC TACTTGCCTC AAGAGAAAGA CCTGAAGATC 
TCTGTCTATG ACTACGATAC CTTCACCCGA GATGAGAAGG TGGGAGAGAC AACCATTGAC 
CTGGAGAACC GCTTCCTTTC CCGGTTCGGG TCTCACTGCG GAATCCCTGA GCAGTACTGT 
GTTTCCGGAG TAAATACCTG GCGAGATCAG CTGAGACCAA CGCAGTTGCT TCAAAACGTT 
GCAAGATTCA AAGGCTTCCC GCCACCAGTT CTTTCTGAAG ACGGAAGCAG AATCCGATAC 
GGAGGTCGAG ACTACCATTT GGATGAGTTT GAGGCCAACA AAATCCTGCA CCAGCACCTT 
GGAGCCCCCG AAGAGCGTCT TGCCCTCCAT ATTCTCAGGA CTCAGGGGCT GGTCCCTGAG 
CACGTGGAGA CAAGGACTTT GCACAGCACC TTCCAGCCCA ATATTTCCCA GGGGAAACTT 
CAGATGTGGG TGGATGTCTT CCCCAAGAGT TTAGGACCAC CAGGCCCCCC TTTCAACATC 
ACACCCAGGA AAGCCAAGAA GTACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT 
ATCTTGGATG AGAAGAGCAT CACTGGGGAG GACATGAGTG ACATCTACGT CAAAGGCTGG 
ATTTCCGGCA GCGAAGAAAA CAAACAGAAA ACAGATGTCC ACTACAGGTC CTTGGATGGT 
GAAGGCAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCTGC TGAGCAGCTC 
TGTATAGTCG CTAAAAAAGA GCATTTCTGG AGTATTGATC AAACAGAATT TCGAGTCCCT 
CCTCGGCTGA TCATTCAGAT ATGGGACAAT GACAAGTTTT CCCTGGACGA CTACTTGGGT 
TTTCTGGAAC TTGACTTACA TCGTACGATC ATCCCAGCCA AGACATCAGA GAAATGCAGT 
TTGGACATGA TTCCAGACCT CAAAGCCATG GACCCTCTTA AAGCTAAGAC TGCCTCACTC 
TTTGAACAGA GGTCCATGAA AGGATGGTGG CCGTGCTACG CAGACAAAGA TGGCACCCGG 
GTGATGGCTG GGAAAGTGGA GATGACACTG GAGGTGCTCA ACGAGAGGGA GGCCGACGAG 
AGGCCAGCCG GGAAGGGGCG GAGTGAACCC AACATGAACC CCAAGCTTGA CCCACCAAAC 
CGACCGGAAA CCTCCTTCCT CTGGTTCACG AACCCTTGCA AGACCATGCG CTTCATCGTG 
TGGCGCCGGT TCAAGTGGGT GATCATCGGC CTGCTTCTGC TGCTCATCCT GTTGCTGTTC 
GTGGCTGTGC TCCTGTACTC TCTTCCGAAC TATTTATCAA TGAAGATTGT GAGGCCGAAT 
GCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( XM_006526994.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527057.1 Mus musculus myoferlin (Myof), transcript variant X1, mRNA.

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CloneID	OMu55485
Clone ID Related Accession (Same CDS sequence)	XM_006526994.1
Accession Version	XM_006526994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAGCTGGG 
GCTGCATCAT ATACAGCAAC TACAGGAGAG ACAGAGGTGG GGTTTGTCCC AACATTCGGA 
CCTTGTTATT TGAACCTTTA CGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAT 
GATGAGCTCA ACTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTCGTGGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TGGAGCCCAT TTCCAGTGAT 
GACCTTCTGG TTGTTGAGAA GTACCAGCGA AGGCGGAAGT ATAGCCTGTC CGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTTTGG CATCAACCAC TCAATACAGC 
CGTGCAGTAT TTGACGGGAA CTACTATTAC TACTTACCTT GGGCCCACAC GAAGCCCGTG 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCACCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATAGAGGCTG TAAAATCAGG GATCCAGGGT 
AAAATTCCTG CAAACCAGTT GGCGGAGGTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACCTTGCC TGTCACAGAA GGAAAAGCCA ACGTCACGGT TCTGGATACT 
CAGATCCGAA AGTTGCGGTC CAGGTTTCTC TCCCAGATAC ACGAGGCAGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGTTGGAAA TCGAGGAGTG GCTTGATAAA 
CTGATGCAGC TTACTGAAGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGTGGAGAGA AAAGACTGGC TTATGCACGA ATCCCTGCCC ACCAAGTCTT GTACTCCACC 
AGCGGTGGGA ACGCCTCTGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AAACCAATGG GCCCAAAGTG CCTGTGGAGC TGCGCGTGAA CATCTGGCTG 
GGTTTAAGTG CTGTGGAAAA GAAGTTCAAT AGCTTCGCGG AAGGCACCTT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTTGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAGTG GGAAGGAGAC TGGGTGGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ACGCAGGTCA CACCGAGTTC ACTGATGAAG TCTACCAGAA TGAGAATCGC 
TACCCCGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CGGATGCGAA TGGAGATAAG 
GCGGCATCAC CCAGTGAGAT GACCTGCCCT CCAGGTTGGG AGTGGGAGGA TGACGCATGG 
ATATATGACA TAAATCGAGC AGTGGATGAG AAAGGCTGGG AGTATGGGAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGGAAACG CAAGAAAGAT CTGACACAGA CGGCTTCTAG TACCGCAAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATATG CTTCGTTAAT CGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGACACC TTTCGCCGCA GGCGCTGGAG GAGGAAAATG 
GCCCCTTCTG AGACACACGG TGCCGCTGCC ATCTTTAAAC TCGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA AGAGAAGGGC CCGGAGAAGC AGAAGCACAG CGCCACCACT 
GTGTTTGGAG CAAACACGCC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTGCGCTGCT ACATTTATCA AGCCAGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GATCCATACG CCCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTCGATG AGGTTGAAAT CTTTGGGGAA 
CCCCAAACAG TTCTACAGAA CCCACCCAAC GTTACCATAG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGTCGAAGC ATTTGCTCTC CCTTGGTGAA ACTGAACTCA 
GAAACGGACA TCACACCTAA GCTTCTCTGG CATCCTGTTA TGAATGGAGA CAAGGCCTGT 
GGGGATGTCC TCGTAACAGC AGAGTTGATT CTGAGGAACA AGGATGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAGGGGAT CAGGCCTGTG 
GTTCAGCTCA CTGCCATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCTG TCACATCACC CAGCCTGGTG GTGGAATGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTTAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCTCCGCTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTTG GGCGGAAGCC TGTGGTTGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAT ATTGTACCAC AACTAAAAGC CTCTCTGATG 
TCTGCACCTC CATGCCGGGA AGTCGTTATT GAAATAGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGCAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AAGGACAGTG 
CATCTGTCGG AAAAGGAGGA AGAAATCGTT GACTGGTGGA GTAAATTTTA TGCTTCCTCT 
GGAGAGCATG AAAAATGTGG ACAGTATATT CAGAAAGGCT ATTCCAAACT CAAGATCTAT 
GACTGTGAAC TGGAGGATGT AGCCGACTTT GAAGGCCTGA CAGATTTCTC AGATACCTTC 
AAGCTGTACA GAGGCAAGTC CGATGAGAAT GAAGATCCAT CTGTGGTAGG AGAGTTTAAA 
GGCTCCTTCC GAATCTACCC CTTGCCGGAT GACCCCAGTG TGCCAGCCCC TCCCAGGCAG 
TTTCGGGAAC TGCCTGACAG TGTCCCACAG GAATGCACTG TTAGGATTTA CATTGTTCAA 
GGCTTACAGC TCCAGCCTCA GGACAACAAT GGCCTGTGTG ATCCTTACAT AAAAATAACC 
TTGGGCAAGA AAGTAATTGA AGACCGAGAC CACTACATTC CCAACACTCT CAACCCAGTG 
TTTGGCAGAA TGTACGAACT GAGCTGCTAC TTGCCTCAAG AGAAAGACCT GAAGATCTCT 
GTCTATGACT ACGATACCTT CACCCGAGAT GAGAAGGTGG GAGAGACAAC CATTGACCTG 
GAGAACCGCT TCCTTTCCCG GTTCGGGTCT CACTGCGGAA TCCCTGAGCA GTACTGTGTT 
TCCGGAGTAA ATACCTGGCG AGATCAGCTG AGACCAACGC AGTTGCTTCA AAACGTTGCA 
AGATTCAAAG GCTTCCCGCC ACCAGTTCTT TCTGAAGACG GAAGCAGAAT CCGATACGGA 
GGTCGAGACT ACCATTTGGA TGAGTTTGAG GCCAACAAAA TCCTGCACCA GCACCTTGGA 
GCCCCCGAAG AGCGTCTTGC CCTCCATATT CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAGACAA GGACTTTGCA CAGCACCTTC CAGCCCAATA TTTCCCAGGG GAAACTTCAG 
ATGTGGGTGG ATGTCTTCCC CAAGAGTTTA GGACCACCAG GCCCCCCTTT CAACATCACA 
CCCAGGAAAG CCAAGAAGTA CTACCTGCGT GTGATCATCT GGAATACCAA GGACGTTATC 
TTGGATGAGA AGAGCATCAC TGGGGAGGAC ATGAGTGACA TCTACGTCAA AGGCTGGATT 
TCCGGCAGCG AAGAAAACAA ACAGAAAACA GATGTCCACT ACAGGTCCTT GGATGGTGAA 
GGCAATTTTA ACTGGAGATT TGTTTTCCCA TTTGACTACC TCCCTGCTGA GCAGCTCTGT 
ATAGTCGCTA AAAAAGAGCA TTTCTGGAGT ATTGATCAAA CAGAATTTCG AGTCCCTCCT 
CGGCTGATCA TTCAGATATG GGACAATGAC AAGTTTTCCC TGGACGACTA CTTGGGTTTT 
CTGGAACTTG ACTTACATCG TACGATCATC CCAGCCAAGA CATCAGAGAA ATGCAGTTTG 
GACATGATTC CAGACCTCAA AGCCATGGAC CCTCTTAAAG CTAAGACTGC CTCACTCTTT 
GAACAGAGGT CCATGAAAGG ATGGTGGCCG TGCTACGCAG ACAAAGATGG CACCCGGGTG 
ATGGCTGGGA AAGTGGAGAT GACACTGGAG GTGCTCAACG AGAGGGAGGC CGACGAGAGG 
CCAGCCGGGA AGGGGCGGAG TGAACCCAAC ATGAACCCCA AGCTTGACCC ACCAAACCGA 
CCGGAAACCT CCTTCCTCTG GTTCACGAAC CCTTGCAAGA CCATGCGCTT CATCGTGTGG 
CGCCGGTTCA AGTGGGTGAT CATCGGCCTG CTTCTGCTGC TCATCCTGTT GCTGTTCGTG 
GCTGTGCTCC TGTACTCTCT TCCGAACTAT TTATCAATGA AGATTGTGAG GCCGAATGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527057.1
CDS	250..6492
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDFSSSGAGAASYTATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	XM_006526994.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myoferlin (Myof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526994.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGATT TCTCATCCTC GGGAGCTGGG 
GCTGCATCAT ATACAGCAAC TACAGGAGAG ACAGAGGTGG GGTTTGTCCC AACATTCGGA 
CCTTGTTATT TGAACCTTTA CGGAAGCCCC AGAGAGTACA CAGGATTTCC AGATCCCTAT 
GATGAGCTCA ACTCAGGAAA GGGAGAAGGG GTGGCCTACA GAGGCAGGAT CTTCGTGGAA 
CTGAACACGT TCCTTGAAAA GAAACCACCA GAAAAGAAGC TGGAGCCCAT TTCCAGTGAT 
GACCTTCTGG TTGTTGAGAA GTACCAGCGA AGGCGGAAGT ATAGCCTGTC CGCCGTGTTC 
CATTCAGCCA CCATGTTGCA AGATGTTGGT GAGGCCATTC AGTTTGAAGT CAGCATTGGG 
AACTATGGCA ACAAGTTTGA TGCAACCTGT AAACCTTTGG CATCAACCAC TCAATACAGC 
CGTGCAGTAT TTGACGGGAA CTACTATTAC TACTTACCTT GGGCCCACAC GAAGCCCGTG 
GTCACCCTGA CTTCCTACTG GGAGGATATT AGTCACCGCC TGGATGCAGT GAACACTCTC 
CTAGTCATGG CAGAACGGCT GCAATCAAAC ATAGAGGCTG TAAAATCAGG GATCCAGGGT 
AAAATTCCTG CAAACCAGTT GGCGGAGGTG TGGCTGAAGC TGATAGATGA AGTTATAGAA 
GACACGAGAT ACACCTTGCC TGTCACAGAA GGAAAAGCCA ACGTCACGGT TCTGGATACT 
CAGATCCGAA AGTTGCGGTC CAGGTTTCTC TCCCAGATAC ACGAGGCAGC TCTGAGGATG 
AGATCTGAAG CCACGGATGT GAAATCCACC TTGTTGGAAA TCGAGGAGTG GCTTGATAAA 
CTGATGCAGC TTACTGAAGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC 
CGTGGAGAGA AAAGACTGGC TTATGCACGA ATCCCTGCCC ACCAAGTCTT GTACTCCACC 
AGCGGTGGGA ACGCCTCTGG AAAATACTGT GGGAAAACCC AAACCATCCT TCTGAAGTAT 
CCACAAGAAA AAACCAATGG GCCCAAAGTG CCTGTGGAGC TGCGCGTGAA CATCTGGCTG 
GGTTTAAGTG CTGTGGAAAA GAAGTTCAAT AGCTTCGCGG AAGGCACCTT CACCGTCTTT 
GCTGAAATGT ATGAAAACCA AGCCCTTGTG TTTGGGAAGT GGGGCACCTC TGGACTGGTG 
GGACGCCATA AATTCTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGAGA ATTCTTTTTA 
CCTCCCAAAG GCTGGGAGTG GGAAGGAGAC TGGGTGGTTG ACCCTGAACG AAGCCTCCTG 
ACTGAGGCTG ACGCAGGTCA CACCGAGTTC ACTGATGAAG TCTACCAGAA TGAGAATCGC 
TACCCCGGAG GCGAGTGGAA GCAGGCAGAG GACACCTATA CGGATGCGAA TGGAGATAAG 
GCGGCATCAC CCAGTGAGAT GACCTGCCCT CCAGGTTGGG AGTGGGAGGA TGACGCATGG 
ATATATGACA TAAATCGAGC AGTGGATGAG AAAGGCTGGG AGTATGGGAT TACCATTCCT 
CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAGA TGTATCACAC TCACAGGCGG 
CGGAGACTGG TCCGGAAACG CAAGAAAGAT CTGACACAGA CGGCTTCTAG TACCGCAAGG 
GCCATGGAAG AACTTGAAGA CCGGGAGGGC TGGGAATATG CTTCGTTAAT CGGCTGGAAA 
TTCCACTGGA AACAGCGCAG TTCAGACACC TTTCGCCGCA GGCGCTGGAG GAGGAAAATG 
GCCCCTTCTG AGACACACGG TGCCGCTGCC ATCTTTAAAC TCGAAGGTGC CCTTGGGGCA 
GACACCACAG AGGATGGAGA AGAGAAGGGC CCGGAGAAGC AGAAGCACAG CGCCACCACT 
GTGTTTGGAG CAAACACGCC CATTGTTTCC TGTAATTTCG ACAGAGTCTA CATCTACCAC 
CTGCGCTGCT ACATTTATCA AGCCAGGAAC CTCATGGCTT TGGACAAAGA CAGCTTTTCA 
GATCCATACG CCCATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCC 
ACCCTGAATC CCACCTGGGA CCAAACCATT ATATTCGATG AGGTTGAAAT CTTTGGGGAA 
CCCCAAACAG TTCTACAGAA CCCACCCAAC GTTACCATAG AACTTTTTGA CAATGACCAA 
GTGGGCAAAG ATGAATTTTT AGGTCGAAGC ATTTGCTCTC CCTTGGTGAA ACTGAACTCA 
GAAACGGACA TCACACCTAA GCTTCTCTGG CATCCTGTTA TGAATGGAGA CAAGGCCTGT 
GGGGATGTCC TCGTAACAGC AGAGTTGATT CTGAGGAACA AGGATGGCTC CAACCTTCCC 
ATCCTCCCCT CTCAAAGAGC ACCAAACTTA TACATGGTCC CTCAGGGGAT CAGGCCTGTG 
GTTCAGCTCA CTGCCATTGA GATCCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG 
ATGGCTTCTG TCACATCACC CAGCCTGGTG GTGGAATGCG GAGGAGAGAG AGTAGAATCG 
GTGGTGATCA AAAGCCTTAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA 
GTGTTCTTGC CCAAGGAAGA ATTGTACATG CCTCCGCTGG TGATCAAGGT CATTGATCAC 
AGGCAGTTTG GGCGGAAGCC TGTGGTTGGC CAATGCACCA TTGACCACCT GGACCGCTTT 
CGGTGTGATC CATATGCAGG AAAGGAGGAT ATTGTACCAC AACTAAAAGC CTCTCTGATG 
TCTGCACCTC CATGCCGGGA AGTCGTTATT GAAATAGAAG ACACCAAACC ATTACTGGCT 
TCTAAGTGCT TAAGCAGCAT GTCAACAGCA CTCAGCAAAA TGGCTTCTCC AAGGACAGTG 
CATCTGTCGG AAAAGGAGGA AGAAATCGTT GACTGGTGGA GTAAATTTTA TGCTTCCTCT 
GGAGAGCATG AAAAATGTGG ACAGTATATT CAGAAAGGCT ATTCCAAACT CAAGATCTAT 
GACTGTGAAC TGGAGGATGT AGCCGACTTT GAAGGCCTGA CAGATTTCTC AGATACCTTC 
AAGCTGTACA GAGGCAAGTC CGATGAGAAT GAAGATCCAT CTGTGGTAGG AGAGTTTAAA 
GGCTCCTTCC GAATCTACCC CTTGCCGGAT GACCCCAGTG TGCCAGCCCC TCCCAGGCAG 
TTTCGGGAAC TGCCTGACAG TGTCCCACAG GAATGCACTG TTAGGATTTA CATTGTTCAA 
GGCTTACAGC TCCAGCCTCA GGACAACAAT GGCCTGTGTG ATCCTTACAT AAAAATAACC 
TTGGGCAAGA AAGTAATTGA AGACCGAGAC CACTACATTC CCAACACTCT CAACCCAGTG 
TTTGGCAGAA TGTACGAACT GAGCTGCTAC TTGCCTCAAG AGAAAGACCT GAAGATCTCT 
GTCTATGACT ACGATACCTT CACCCGAGAT GAGAAGGTGG GAGAGACAAC CATTGACCTG 
GAGAACCGCT TCCTTTCCCG GTTCGGGTCT CACTGCGGAA TCCCTGAGCA GTACTGTGTT 
TCCGGAGTAA ATACCTGGCG AGATCAGCTG AGACCAACGC AGTTGCTTCA AAACGTTGCA 
AGATTCAAAG GCTTCCCGCC ACCAGTTCTT TCTGAAGACG GAAGCAGAAT CCGATACGGA 
GGTCGAGACT ACCATTTGGA TGAGTTTGAG GCCAACAAAA TCCTGCACCA GCACCTTGGA 
GCCCCCGAAG AGCGTCTTGC CCTCCATATT CTCAGGACTC AGGGGCTGGT CCCTGAGCAC 
GTGGAGACAA GGACTTTGCA CAGCACCTTC CAGCCCAATA TTTCCCAGGG GAAACTTCAG 
ATGTGGGTGG ATGTCTTCCC CAAGAGTTTA GGACCACCAG GCCCCCCTTT CAACATCACA 
CCCAGGAAAG CCAAGAAGTA CTACCTGCGT GTGATCATCT GGAATACCAA GGACGTTATC 
TTGGATGAGA AGAGCATCAC TGGGGAGGAC ATGAGTGACA TCTACGTCAA AGGCTGGATT 
TCCGGCAGCG AAGAAAACAA ACAGAAAACA GATGTCCACT ACAGGTCCTT GGATGGTGAA 
GGCAATTTTA ACTGGAGATT TGTTTTCCCA TTTGACTACC TCCCTGCTGA GCAGCTCTGT 
ATAGTCGCTA AAAAAGAGCA TTTCTGGAGT ATTGATCAAA CAGAATTTCG AGTCCCTCCT 
CGGCTGATCA TTCAGATATG GGACAATGAC AAGTTTTCCC TGGACGACTA CTTGGGTTTT 
CTGGAACTTG ACTTACATCG TACGATCATC CCAGCCAAGA CATCAGAGAA ATGCAGTTTG 
GACATGATTC CAGACCTCAA AGCCATGGAC CCTCTTAAAG CTAAGACTGC CTCACTCTTT 
GAACAGAGGT CCATGAAAGG ATGGTGGCCG TGCTACGCAG ACAAAGATGG CACCCGGGTG 
ATGGCTGGGA AAGTGGAGAT GACACTGGAG GTGCTCAACG AGAGGGAGGC CGACGAGAGG 
CCAGCCGGGA AGGGGCGGAG TGAACCCAAC ATGAACCCCA AGCTTGACCC ACCAAACCGA 
CCGGAAACCT CCTTCCTCTG GTTCACGAAC CCTTGCAAGA CCATGCGCTT CATCGTGTGG 
CGCCGGTTCA AGTGGGTGAT CATCGGCCTG CTTCTGCTGC TCATCCTGTT GCTGTTCGTG 
GCTGTGCTCC TGTACTCTCT TCCGAACTAT TTATCAATGA AGATTGTGAG GCCGAATGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myof ( XM_006526995.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527058.1 Mus musculus myoferlin (Myof), transcript variant X2, mRNA.

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CloneID	OMu55486
Clone ID Related Accession (Same CDS sequence)	XM_006526995.1
Accession Version	XM_006526995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA CTACAGGAGA GACAGAGGTG 
GGGTTTGTCC CAACATTCGG ACCTTGTTAT TTGAACCTTT ACGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA TGATGAGCTC AACTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTCGTGGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTGGAGCCCA TTTCCAGTGA TGACCTTCTG GTTGTTGAGA AGTACCAGCG AAGGCGGAAG 
TATAGCCTGT CCGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTTTG 
GCATCAACCA CTCAATACAG CCGTGCAGTA TTTGACGGGA ACTACTATTA CTACTTACCT 
TGGGCCCACA CGAAGCCCGT GGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCACCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATAGAGGCT 
GTAAAATCAG GGATCCAGGG TAAAATTCCT GCAAACCAGT TGGCGGAGGT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACCTTGC CTGTCACAGA AGGAAAAGCC 
AACGTCACGG TTCTGGATAC TCAGATCCGA AAGTTGCGGT CCAGGTTTCT CTCCCAGATA 
CACGAGGCAG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGTTGGAA 
ATCGAGGAGT GGCTTGATAA ACTGATGCAG CTTACTGAAG AGCCTCAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGTGGAGAG AAAAGACTGG CTTATGCACG AATCCCTGCC 
CACCAAGTCT TGTACTCCAC CAGCGGTGGG AACGCCTCTG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAAACCAATG GGCCCAAAGT GCCTGTGGAG 
CTGCGCGTGA ACATCTGGCT GGGTTTAAGT GCTGTGGAAA AGAAGTTCAA TAGCTTCGCG 
GAAGGCACCT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTTGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAGT GGGAAGGAGA CTGGGTGGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GACGCAGGTC ACACCGAGTT CACTGATGAA 
GTCTACCAGA ATGAGAATCG CTACCCCGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACGGATGCGA ATGGAGATAA GGCGGCATCA CCCAGTGAGA TGACCTGCCC TCCAGGTTGG 
GAGTGGGAGG ATGACGCATG GATATATGAC ATAAATCGAG CAGTGGATGA GAAAGGCTGG 
GAGTATGGGA TTACCATTCC TCCTGATAAT AAGCCCAAAT CCTGGGTTGC AGCAGAAAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGGAAAC GCAAGAAAGA TCTGACACAG 
ACGGCTTCTA GTACCGCAAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAT 
GCTTCGTTAA TCGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGACAC CTTTCGCCGC 
AGGCGCTGGA GGAGGAAAAT GGCCCCTTCT GAGACACACG GTGCCGCTGC CATCTTTAAA 
CTCGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG AAGAGAAGGG CCCGGAGAAG 
CAGAAGCACA GCGCCACCAC TGTGTTTGGA GCAAACACGC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTGCGCTGC TACATTTATC AAGCCAGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGATCCATAC GCCCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATCCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTCGAT 
GAGGTTGAAA TCTTTGGGGA ACCCCAAACA GTTCTACAGA ACCCACCCAA CGTTACCATA 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGTCGAAG CATTTGCTCT 
CCCTTGGTGA AACTGAACTC AGAAACGGAC ATCACACCTA AGCTTCTCTG GCATCCTGTT 
ATGAATGGAG ACAAGGCCTG TGGGGATGTC CTCGTAACAG CAGAGTTGAT TCTGAGGAAC 
AAGGATGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAGGGGA TCAGGCCTGT GGTTCAGCTC ACTGCCATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCT GTCACATCAC CCAGCCTGGT GGTGGAATGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTTA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCTCCGCTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTT GGGCGGAAGC CTGTGGTTGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA TATTGTACCA 
CAACTAAAAG CCTCTCTGAT GTCTGCACCT CCATGCCGGG AAGTCGTTAT TGAAATAGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGCA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAAGGACAGT GCATCTGTCG GAAAAGGAGG AAGAAATCGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCTC TGGAGAGCAT GAAAAATGTG GACAGTATAT TCAGAAAGGC 
TATTCCAAAC TCAAGATCTA TGACTGTGAA CTGGAGGATG TAGCCGACTT TGAAGGCCTG 
ACAGATTTCT CAGATACCTT CAAGCTGTAC AGAGGCAAGT CCGATGAGAA TGAAGATCCA 
TCTGTGGTAG GAGAGTTTAA AGGCTCCTTC CGAATCTACC CCTTGCCGGA TGACCCCAGT 
GTGCCAGCCC CTCCCAGGCA GTTTCGGGAA CTGCCTGACA GTGTCCCACA GGAATGCACT 
GTTAGGATTT ACATTGTTCA AGGCTTACAG CTCCAGCCTC AGGACAACAA TGGCCTGTGT 
GATCCTTACA TAAAAATAAC CTTGGGCAAG AAAGTAATTG AAGACCGAGA CCACTACATT 
CCCAACACTC TCAACCCAGT GTTTGGCAGA ATGTACGAAC TGAGCTGCTA CTTGCCTCAA 
GAGAAAGACC TGAAGATCTC TGTCTATGAC TACGATACCT TCACCCGAGA TGAGAAGGTG 
GGAGAGACAA CCATTGACCT GGAGAACCGC TTCCTTTCCC GGTTCGGGTC TCACTGCGGA 
ATCCCTGAGC AGTACTGTGT TTCCGGAGTA AATACCTGGC GAGATCAGCT GAGACCAACG 
CAGTTGCTTC AAAACGTTGC AAGATTCAAA GGCTTCCCGC CACCAGTTCT TTCTGAAGAC 
GGAAGCAGAA TCCGATACGG AGGTCGAGAC TACCATTTGG ATGAGTTTGA GGCCAACAAA 
ATCCTGCACC AGCACCTTGG AGCCCCCGAA GAGCGTCTTG CCCTCCATAT TCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAGACA AGGACTTTGC ACAGCACCTT CCAGCCCAAT 
ATTTCCCAGG GGAAACTTCA GATGTGGGTG GATGTCTTCC CCAAGAGTTT AGGACCACCA 
GGCCCCCCTT TCAACATCAC ACCCAGGAAA GCCAAGAAGT ACTACCTGCG TGTGATCATC 
TGGAATACCA AGGACGTTAT CTTGGATGAG AAGAGCATCA CTGGGGAGGA CATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TTCCGGCAGC GAAGAAAACA AACAGAAAAC AGATGTCCAC 
TACAGGTCCT TGGATGGTGA AGGCAATTTT AACTGGAGAT TTGTTTTCCC ATTTGACTAC 
CTCCCTGCTG AGCAGCTCTG TATAGTCGCT AAAAAAGAGC ATTTCTGGAG TATTGATCAA 
ACAGAATTTC GAGTCCCTCC TCGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCC 
CTGGACGACT ACTTGGGTTT TCTGGAACTT GACTTACATC GTACGATCAT CCCAGCCAAG 
ACATCAGAGA AATGCAGTTT GGACATGATT CCAGACCTCA AAGCCATGGA CCCTCTTAAA 
GCTAAGACTG CCTCACTCTT TGAACAGAGG TCCATGAAAG GATGGTGGCC GTGCTACGCA 
GACAAAGATG GCACCCGGGT GATGGCTGGG AAAGTGGAGA TGACACTGGA GGTGCTCAAC 
GAGAGGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGA GTGAACCCAA CATGAACCCC 
AAGCTTGACC CACCAAACCG ACCGGAAACC TCCTTCCTCT GGTTCACGAA CCCTTGCAAG 
ACCATGCGCT TCATCGTGTG GCGCCGGTTC AAGTGGGTGA TCATCGGCCT GCTTCTGCTG 
CTCATCCTGT TGCTGTTCGT GGCTGTGCTC CTGTACTCTC TTCCGAACTA TTTATCAATG 
AAGATTGTGA GGCCGAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527058.1
CDS	250..6453
Translation	MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKCLSSMSTALSKMASPRTVHLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Target ORF information:

RefSeq Version	XM_006526995.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myoferlin (Myof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526995.1

ORF Insert Sequence:

ATGCTTCGAG TGATTGTGGA ATCTGCCACT AACATCCCTA AAACAAAATT TGGGAAGCCG 
GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATTTTG GAATTTGACT TGAGGGGGAT ACCACTGGAC 
TCTTCATCCT CCCTGGTGAT TGTTGTCAAA GATTTTGAGA CAATTGGACA AAATAAATTA 
ATTGGCACAG CAACTGTCTC CCTGAAGGAC CTGATTGGGG ACCAGAACAG GTCTTTGCCC 
TACAAACAGA CCTCCCTGCT AAATGAAAAG GGACAAGACA CTGGGGCCAC CATTGACCTG 
GTGATCGGCT ATACCCCACC TTCAGCTCCA CATCCAAATG ATCCGAGCGG GACCAGTGTG 
CCAGGCATGG GAGAAGAAGA GGAAGAAGAT CAAGGAGATG AAGACAGGGT AGATGGCATA 
GTCAGAGGCC CTGGGCCCAA GGGGCCATCT GGGACAGTGT CAGAAGCTCA GCTGGCTCGG 
AGGATCACCA AAGGGAAGAG CAGCCGAAGG ATGCTGTCCA ACAAGCCACA GGATTTCCAG 
ATCCGTGTCA GAGTGATCGA GGGCCGTCAG TTATGCGGCA ACAACATAAG GCCCGTGGTG 
AAAGTTCACA TCTGTGGTCA GACGCACCGC ACGAGAATCA AGAGAGGGAA CAATCCGTTT 
TTTGACGAGT TATTTTTCTA CAATGTGCAC ATCACACCCT CTGAGCTCAT GGATGAGATC 
ATCAGTATCC GGGTTTATAA TTCCCATTCT TTGAGGGCAG ATTGTCTGAT GGGGGAATTT 
AAGATTGATG TTGGATTTGT TTATGATGAA CCTGGCCACG CTGTCATGAG GAAATGGCTC 
CTTCTCAATG ACCCAGAAGA CACCAGCTCA GGCGCAAAAG GTTACATGAA AGTCAGCATG 
TTTGTCCTGG GAACCGGAGA CGAACCCCCT CCTGAGAAAC GAGACCGTGA CAATGACAGT 
GATGACGTGG AGAGCAACTT GTTACTTCCG GCAGGCATTG CCCTCCGGTG GGTGACCTTC 
ATGCTGAAGA TCTACCGAGC CGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAAACA 
GTGAAGGAAA TATTTGGTGG CAACGCAGAT AAAAAAAATC TTGTGGATCC TTTTGTAGAA 
GTCTCCTTTG CTGGGAAAAA GGTGTGCACA AATATAATTG AGCGGAATGC AAACCCTGAA 
TGGAATCAAG TTGTCAACCT TCAGATCAAG TTTCCCTCCA TGTGTGAAAA AATAAAACTA 
ACAGTATATG ACTGGGACCG GCTTACTAAA AATGATGTAG TTGGGACCAC GTACCTGTAC 
CTCTCTAAAA TTGCTGCCTC TGGGGGAGAA GTGGAAGCAA CTACAGGAGA GACAGAGGTG 
GGGTTTGTCC CAACATTCGG ACCTTGTTAT TTGAACCTTT ACGGAAGCCC CAGAGAGTAC 
ACAGGATTTC CAGATCCCTA TGATGAGCTC AACTCAGGAA AGGGAGAAGG GGTGGCCTAC 
AGAGGCAGGA TCTTCGTGGA ACTGAACACG TTCCTTGAAA AGAAACCACC AGAAAAGAAG 
CTGGAGCCCA TTTCCAGTGA TGACCTTCTG GTTGTTGAGA AGTACCAGCG AAGGCGGAAG 
TATAGCCTGT CCGCCGTGTT CCATTCAGCC ACCATGTTGC AAGATGTTGG TGAGGCCATT 
CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ATGCAACCTG TAAACCTTTG 
GCATCAACCA CTCAATACAG CCGTGCAGTA TTTGACGGGA ACTACTATTA CTACTTACCT 
TGGGCCCACA CGAAGCCCGT GGTCACCCTG ACTTCCTACT GGGAGGATAT TAGTCACCGC 
CTGGATGCAG TGAACACTCT CCTAGTCATG GCAGAACGGC TGCAATCAAA CATAGAGGCT 
GTAAAATCAG GGATCCAGGG TAAAATTCCT GCAAACCAGT TGGCGGAGGT GTGGCTGAAG 
CTGATAGATG AAGTTATAGA AGACACGAGA TACACCTTGC CTGTCACAGA AGGAAAAGCC 
AACGTCACGG TTCTGGATAC TCAGATCCGA AAGTTGCGGT CCAGGTTTCT CTCCCAGATA 
CACGAGGCAG CTCTGAGGAT GAGATCTGAA GCCACGGATG TGAAATCCAC CTTGTTGGAA 
ATCGAGGAGT GGCTTGATAA ACTGATGCAG CTTACTGAAG AGCCTCAGAA CAGCATGCCT 
GACATCATCA TCTGGATGAT CCGTGGAGAG AAAAGACTGG CTTATGCACG AATCCCTGCC 
CACCAAGTCT TGTACTCCAC CAGCGGTGGG AACGCCTCTG GAAAATACTG TGGGAAAACC 
CAAACCATCC TTCTGAAGTA TCCACAAGAA AAAACCAATG GGCCCAAAGT GCCTGTGGAG 
CTGCGCGTGA ACATCTGGCT GGGTTTAAGT GCTGTGGAAA AGAAGTTCAA TAGCTTCGCG 
GAAGGCACCT TCACCGTCTT TGCTGAAATG TATGAAAACC AAGCCCTTGT GTTTGGGAAG 
TGGGGCACCT CTGGACTGGT GGGACGCCAT AAATTCTCTG ATGTCACAGG AAAAATAAAA 
CTCAAGAGAG AATTCTTTTT ACCTCCCAAA GGCTGGGAGT GGGAAGGAGA CTGGGTGGTT 
GACCCTGAAC GAAGCCTCCT GACTGAGGCT GACGCAGGTC ACACCGAGTT CACTGATGAA 
GTCTACCAGA ATGAGAATCG CTACCCCGGA GGCGAGTGGA AGCAGGCAGA GGACACCTAT 
ACGGATGCGA ATGGAGATAA GGCGGCATCA CCCAGTGAGA TGACCTGCCC TCCAGGTTGG 
GAGTGGGAGG ATGACGCATG GATATATGAC ATAAATCGAG CAGTGGATGA GAAAGGCTGG 
GAGTATGGGA TTACCATTCC TCCTGATAAT AAGCCCAAAT CCTGGGTTGC AGCAGAAAAG 
ATGTATCACA CTCACAGGCG GCGGAGACTG GTCCGGAAAC GCAAGAAAGA TCTGACACAG 
ACGGCTTCTA GTACCGCAAG GGCCATGGAA GAACTTGAAG ACCGGGAGGG CTGGGAATAT 
GCTTCGTTAA TCGGCTGGAA ATTCCACTGG AAACAGCGCA GTTCAGACAC CTTTCGCCGC 
AGGCGCTGGA GGAGGAAAAT GGCCCCTTCT GAGACACACG GTGCCGCTGC CATCTTTAAA 
CTCGAAGGTG CCCTTGGGGC AGACACCACA GAGGATGGAG AAGAGAAGGG CCCGGAGAAG 
CAGAAGCACA GCGCCACCAC TGTGTTTGGA GCAAACACGC CCATTGTTTC CTGTAATTTC 
GACAGAGTCT ACATCTACCA CCTGCGCTGC TACATTTATC AAGCCAGGAA CCTCATGGCT 
TTGGACAAAG ACAGCTTTTC AGATCCATAC GCCCATGTCT CCTTCCTCCA TCGAAGCAAA 
ACCACTGAGA TCATCCACTC CACCCTGAAT CCCACCTGGG ACCAAACCAT TATATTCGAT 
GAGGTTGAAA TCTTTGGGGA ACCCCAAACA GTTCTACAGA ACCCACCCAA CGTTACCATA 
GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGTCGAAG CATTTGCTCT 
CCCTTGGTGA AACTGAACTC AGAAACGGAC ATCACACCTA AGCTTCTCTG GCATCCTGTT 
ATGAATGGAG ACAAGGCCTG TGGGGATGTC CTCGTAACAG CAGAGTTGAT TCTGAGGAAC 
AAGGATGGCT CCAACCTTCC CATCCTCCCC TCTCAAAGAG CACCAAACTT ATACATGGTC 
CCTCAGGGGA TCAGGCCTGT GGTTCAGCTC ACTGCCATTG AGATCCTAGC TTGGGGCTTA 
AGAAATATGA AAAACTACCA GATGGCTTCT GTCACATCAC CCAGCCTGGT GGTGGAATGC 
GGAGGAGAGA GAGTAGAATC GGTGGTGATC AAAAGCCTTA AGAAGACACC CAACTTCCCA 
AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAAG AATTGTACAT GCCTCCGCTG 
GTGATCAAGG TCATTGATCA CAGGCAGTTT GGGCGGAAGC CTGTGGTTGG CCAATGCACC 
ATTGACCACC TGGACCGCTT TCGGTGTGAT CCATATGCAG GAAAGGAGGA TATTGTACCA 
CAACTAAAAG CCTCTCTGAT GTCTGCACCT CCATGCCGGG AAGTCGTTAT TGAAATAGAA 
GACACCAAAC CATTACTGGC TTCTAAGTGC TTAAGCAGCA TGTCAACAGC ACTCAGCAAA 
ATGGCTTCTC CAAGGACAGT GCATCTGTCG GAAAAGGAGG AAGAAATCGT TGACTGGTGG 
AGTAAATTTT ATGCTTCCTC TGGAGAGCAT GAAAAATGTG GACAGTATAT TCAGAAAGGC 
TATTCCAAAC TCAAGATCTA TGACTGTGAA CTGGAGGATG TAGCCGACTT TGAAGGCCTG 
ACAGATTTCT CAGATACCTT CAAGCTGTAC AGAGGCAAGT CCGATGAGAA TGAAGATCCA 
TCTGTGGTAG GAGAGTTTAA AGGCTCCTTC CGAATCTACC CCTTGCCGGA TGACCCCAGT 
GTGCCAGCCC CTCCCAGGCA GTTTCGGGAA CTGCCTGACA GTGTCCCACA GGAATGCACT 
GTTAGGATTT ACATTGTTCA AGGCTTACAG CTCCAGCCTC AGGACAACAA TGGCCTGTGT 
GATCCTTACA TAAAAATAAC CTTGGGCAAG AAAGTAATTG AAGACCGAGA CCACTACATT 
CCCAACACTC TCAACCCAGT GTTTGGCAGA ATGTACGAAC TGAGCTGCTA CTTGCCTCAA 
GAGAAAGACC TGAAGATCTC TGTCTATGAC TACGATACCT TCACCCGAGA TGAGAAGGTG 
GGAGAGACAA CCATTGACCT GGAGAACCGC TTCCTTTCCC GGTTCGGGTC TCACTGCGGA 
ATCCCTGAGC AGTACTGTGT TTCCGGAGTA AATACCTGGC GAGATCAGCT GAGACCAACG 
CAGTTGCTTC AAAACGTTGC AAGATTCAAA GGCTTCCCGC CACCAGTTCT TTCTGAAGAC 
GGAAGCAGAA TCCGATACGG AGGTCGAGAC TACCATTTGG ATGAGTTTGA GGCCAACAAA 
ATCCTGCACC AGCACCTTGG AGCCCCCGAA GAGCGTCTTG CCCTCCATAT TCTCAGGACT 
CAGGGGCTGG TCCCTGAGCA CGTGGAGACA AGGACTTTGC ACAGCACCTT CCAGCCCAAT 
ATTTCCCAGG GGAAACTTCA GATGTGGGTG GATGTCTTCC CCAAGAGTTT AGGACCACCA 
GGCCCCCCTT TCAACATCAC ACCCAGGAAA GCCAAGAAGT ACTACCTGCG TGTGATCATC 
TGGAATACCA AGGACGTTAT CTTGGATGAG AAGAGCATCA CTGGGGAGGA CATGAGTGAC 
ATCTACGTCA AAGGCTGGAT TTCCGGCAGC GAAGAAAACA AACAGAAAAC AGATGTCCAC 
TACAGGTCCT TGGATGGTGA AGGCAATTTT AACTGGAGAT TTGTTTTCCC ATTTGACTAC 
CTCCCTGCTG AGCAGCTCTG TATAGTCGCT AAAAAAGAGC ATTTCTGGAG TATTGATCAA 
ACAGAATTTC GAGTCCCTCC TCGGCTGATC ATTCAGATAT GGGACAATGA CAAGTTTTCC 
CTGGACGACT ACTTGGGTTT TCTGGAACTT GACTTACATC GTACGATCAT CCCAGCCAAG 
ACATCAGAGA AATGCAGTTT GGACATGATT CCAGACCTCA AAGCCATGGA CCCTCTTAAA 
GCTAAGACTG CCTCACTCTT TGAACAGAGG TCCATGAAAG GATGGTGGCC GTGCTACGCA 
GACAAAGATG GCACCCGGGT GATGGCTGGG AAAGTGGAGA TGACACTGGA GGTGCTCAAC 
GAGAGGGAGG CCGACGAGAG GCCAGCCGGG AAGGGGCGGA GTGAACCCAA CATGAACCCC 
AAGCTTGACC CACCAAACCG ACCGGAAACC TCCTTCCTCT GGTTCACGAA CCCTTGCAAG 
ACCATGCGCT TCATCGTGTG GCGCCGGTTC AAGTGGGTGA TCATCGGCCT GCTTCTGCTG 
CTCATCCTGT TGCTGTTCGT GGCTGTGCTC CTGTACTCTC TTCCGAACTA TTTATCAATG 
AAGATTGTGA GGCCGAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.
Scientific reports620727(2016 Feb)
Bhattacharyya S,Rainey MA,Arya P,Dutta S,George M,Storck MD,McComb RD,Muirhead D,Todd GL,Gould K,Datta K,Gelineau-van Waes J,Band V,Band H

Myoferlin regulation by NFAT in muscle injury, regeneration and repair.
Journal of cell science123(Pt 14)2413-22(2010 Jul)
Demonbreun AR,Lapidos KA,Heretis K,Levin S,Dale R,Pytel P,Svensson EC,McNally EM

Myoferlin is required for insulin-like growth factor response and muscle growth.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(4)1284-95(2010 Apr)
Demonbreun AR,Posey AD,Heretis K,Swaggart KA,Earley JU,Pytel P,McNally EM

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Myof cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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