Vkorc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vkorc1
Entrez Gene ID	309004
Full Name	vitamin K epoxide reductase complex, subunit 1
General protein information	Preferred Names vitamin K epoxide reductase complex, subunit 1 Names vitamin K epoxide reductase complex subunit 1 Warfarin resistance phylloquinone epoxide reductase vitamin K1 2,3-epoxide reductase subunit 1 vitamin K1 epoxide reductase (warfarin-sensitive)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q37
Summary	Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_203335.2	NP_976080.1	vitamin K epoxide reductase complex subunit 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vkorc1	NP_976080.1
Canis lupus familiaris (dog)	VKORC1	XP_005621301.1
Bos taurus (cattle)	VKORC1	NP_001003903.1
Drosophila melanogaster (fruit fly)	Vkor	NP_001014533.1
Pan troglodytes (chimpanzee)	VKORC1	NP_001238859.1
Homo sapiens (human)	VKORC1	NP_076869.1
Mus musculus (house mouse)	Vkorc1	NP_848715.1
Danio rerio (zebrafish)	vkorc1	XP_001336460.1

Gene Ontology
Bibliography

Related articles in PubMed

Elevated difenacoum metabolism involved in the difenacoum-resistant phenotype observed in Berkshire rats homozygous for the L120Q mutation in Vkorc1 gene.
Boitet M, Hammed A, Chatron N, Debaux JV, Benoit E, Lattard V
Pest management science(2017 Nov)

Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.
McCabe KM, Booth SL, Fu X, Ward E, Adams MA, Holden RM
American journal of nephrology45(1)4-13(2017)

Structural and functional insights into enzymes of the vitamin K cycle.
Tie JK, Stafford DW
Journal of thrombosis and haemostasis : JTH14(2)236-47(2016 Feb)

Structural Modeling Insights into Human VKORC1 Phenotypes.
Czogalla KJ, Watzka M, Oldenburg J
Nutrients7(8)6837-51(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In a rat model of chronic kidney disease, there was a decrease in the thoracic aorta expression of Vkor and Ggcx enzymes, and a decrease in the kidney level of vitamin K1 to MK-4 bioconversion enzyme Ubiad1.
Title: Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.

The aim of this study was to determine the distribution of VKORC1 1173C > T and VKORC1 -1639G > A polymorphisms, which are most important for warfarin therapy
Title: Vitamin K epoxide reductase complex 1 (VKORC1) gene polymorphisms in population of Eastern Croatia.

The involvement of VKORC1L1 in VKOR activity partly explains the low susceptibility of some extrahepatic tissues to vitamin K antagonists.
Title: VKORC1L1, an enzyme rescuing the vitamin K 2,3-epoxide reductase activity in some extrahepatic tissues during anticoagulation therapy.

The differences in kinetics of VKOR activity between warfarin-resistant and sensitive rats were studied.
Title: A novel mutation in VKORC1 and its effect on enzymatic activity in Japanese warfarin-resistant rats.

An analysis of novel mutations show that the VKORC1 gene is the main target for spontaneous mutations conferring warfarin resistance.
Title: Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin?

The following Vkorc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vkorc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13545	NM_203335.2 Latest version!	Rattus norvegicus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vkorc1 ( NM_203335.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_976080.1 Rattus norvegicus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.

Price & Availability↑

CloneID	ORa13545
Clone ID Related Accession (Same CDS sequence)	NM_203335.2
Accession Version	NM_203335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	vitamin K epoxide reductase complex subunit 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB314647.1, AY423047.1 and AW253787.1. On or before Mar 30, 2004 this sequence version replaced XM_219366.2, NM_203335.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ210919.1, EV779143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCA CCTGGAGGAG CCCTGGACGT TTGCGGCTTG CACTATGCCT CGCTGGCCTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCAATGA GGATTACCGC 
GCGCTCTGCG ACGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTGGGGC 
CGGGGCTTTG GGCTGGTGGA GCATGTGTTA GGAGCTGACA GCATCCTCAA CCAATCCAAC 
AGCATATTTG GTTGCATGTT CTACACCATA CAGCTGTTGT TAGGTTGCTT GAGGGGACGT 
TGGGCCTCTA TCCTACTGAT CCTGAGTTCC CTGGTGTCTG TCGCTGGTTC TCTGTACCTG 
GCCTGGATCC TGTTCTTTGT CCTGTATGAT TTCTGCATTG TTTGCATCAC CACCTATGCC 
ATCAATGCGG GCCTGATGTT GCTTAGCTTC CAGAAGGTGC CAGAACACAA GGTCAAAAAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976080.1
CDS	23..508
Translation	MGTTWRSPGRLRLALCLAGLALSLYALHVKAARARNEDYRALCDVGTAISCSRVFSSRWGRGFGLVEHVLGADSILNQSNSIFGCMFYTIQLLLGCLRGRWASILLILSSLVSVAGSLYLAWILFFVLYDFCIVCITTYAINAGLMLLSFQKVPEHKVKKP

Target ORF information:

RefSeq Version	NM_203335.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203335.2

ORF Insert Sequence:

ATGGGCACCA CCTGGAGGAG CCCTGGACGT TTGCGGCTTG CACTATGCCT CGCTGGCCTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCAATGA GGATTACCGC 
GCGCTCTGCG ACGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTGGGGC 
CGGGGCTTTG GGCTGGTGGA GCATGTGTTA GGAGCTGACA GCATCCTCAA CCAATCCAAC 
AGCATATTTG GTTGCATGTT CTACACCATA CAGCTGTTGT TAGGTTGCTT GAGGGGACGT 
TGGGCCTCTA TCCTACTGAT CCTGAGTTCC CTGGTGTCTG TCGCTGGTTC TCTGTACCTG 
GCCTGGATCC TGTTCTTTGT CCTGTATGAT TTCTGCATTG TTTGCATCAC CACCTATGCC 
ATCAATGCGG GCCTGATGTT GCTTAGCTTC CAGAAGGTGC CAGAACACAA GGTCAAAAAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elevated difenacoum metabolism involved in the difenacoum-resistant phenotype observed in Berkshire rats homozygous for the L120Q mutation in Vkorc1 gene.
Pest management science(2017 Nov)
Boitet M,Hammed A,Chatron N,Debaux JV,Benoit E,Lattard V

Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.
American journal of nephrology45(1)4-13(2017)
McCabe KM,Booth SL,Fu X,Ward E,Adams MA,Holden RM

Structural and functional insights into enzymes of the vitamin K cycle.
Journal of thrombosis and haemostasis : JTH14(2)236-47(2016 Feb)
Tie JK,Stafford DW

Structural Modeling Insights into Human VKORC1 Phenotypes.
Nutrients7(8)6837-51(2015 Aug)
Czogalla KJ,Watzka M,Oldenburg J

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Vkorc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報