Vkorc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vkorc1
Entrez Gene ID	27973
Full Name	vitamin K epoxide reductase complex, subunit 1
Synonyms	D7Wsu86e
General protein information	Preferred Names vitamin K epoxide reductase complex, subunit 1 Names vitamin K epoxide reductase complex subunit 1 phylloquinone epoxide reductase vitamin K 2,3-epoxide reductase complex subunit 1 vitamin K1 2,3-epoxide reductase subunit 1 vitamin K1 epoxide reductase (warfarin-sensitive)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F3\|7 69.81 cM
Summary	Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178600.2	NP_848715.1	vitamin K epoxide reductase complex subunit 1 precursor
XM_030242648.1	XP_030098508.1	vitamin K epoxide reductase complex subunit 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vkorc1	NP_976080.1
Canis lupus familiaris (dog)	VKORC1	XP_005621301.1
Bos taurus (cattle)	VKORC1	NP_001003903.1
Drosophila melanogaster (fruit fly)	Vkor	NP_001014533.1
Pan troglodytes (chimpanzee)	VKORC1	NP_001238859.1
Homo sapiens (human)	VKORC1	NP_076869.1
Mus musculus (house mouse)	Vkorc1	NP_848715.1
Danio rerio (zebrafish)	vkorc1	XP_001336460.1

Gene Ontology
Bibliography

Related articles in PubMed

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
Lacombe J, Rishavy MA, Berkner KL, Ferron M
JCI insight3(1)(2018 Jan)

Two enzymes catalyze vitamin K 2,3-epoxide reductase activity in mouse: VKORC1 is highly expressed in exocrine tissues while VKORC1L1 is highly expressed in brain.
Caspers M, Czogalla KJ, Liphardt K, M?ller J, Westhofen P, Watzka M, Oldenburg J
Thrombosis research135(5)977-83(2015 May)

GGCX and VKORC1 inhibit osteocalcin endocrine functions.
Ferron M, Lacombe J, Germain A, Oury F, Karsenty G
The Journal of cell biology208(6)761-76(2015 Mar)

VKORC1L1, an enzyme rescuing the vitamin K 2,3-epoxide reductase activity in some extrahepatic tissues during anticoagulation therapy.
Hammed A, Matagrin B, Spohn G, Prouillac C, Benoit E, Lattard V
The Journal of biological chemistry288(40)28733-42(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

quantified mRNA levels for VKORC1, VKORC1L1, GGCX, and NQO1 and measured VKOR enzymatic activities in 29 different tissues
Title: Two enzymes catalyze vitamin K 2,3-epoxide reductase activity in mouse: VKORC1 is highly expressed in exocrine tissues while VKORC1L1 is highly expressed in brain.

OCN is gamma-carboxylated by the gamma-carboxylase (GGCX) on three glutamic acid residues, a cellular process requiring reduction of vitamin K by a second enzyme, VKORC1.
Title: GGCX and VKORC1 inhibit osteocalcin endocrine functions.

The involvement of VKORC1L1 in VKOR activity partly explains the low susceptibility of some extrahepatic tissues to vitamin K antagonists.
Title: VKORC1L1, an enzyme rescuing the vitamin K 2,3-epoxide reductase activity in some extrahepatic tissues during anticoagulation therapy.

The three most frequently found sequence variants are associated with a substantial loss of rodenticide efficacy of first-generation anticoagulants, as well as the second-generation compound bromadiolone and most probably also difenacoum.
Title: Distribution and frequency of VKORC1 sequence variants conferring resistance to anticoagulants in Mus musculus.

role in vitamin K-dependent gamma-glutamyl carboxylation; the knockout mice die within 20 days after birth due to intracerebral haemorrhage
Title: VKORC1 deficiency in mice causes early postnatal lethality due to severe bleeding.

The following Vkorc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vkorc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22018	NM_178600.2 Latest version!	Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu89517	XM_030242648.1 Latest version!	Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vkorc1 ( NM_178600.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848715.1 Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.

Price & Availability↑

CloneID	OMu22018
Clone ID Related Accession (Same CDS sequence)	NM_178600.2
Accession Version	NM_178600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	vitamin K epoxide reductase complex subunit 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK003237.1 and CD774913.1. On Mar 30, 2004 this sequence version replaced NM_178600.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV062359.1, BU558856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCA CCTGGAGGAG CCCTGGACTC GTGCGGCTTG CACTGTGCCT CGCTGGCTTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCGATGA AAATTACCGC 
GCGCTCTGCG ATGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTGGGGC 
CGGGGCTTTG GGCTGGTGGA GCACATGCTA GGAGCGGACA GCGTCCTCAA CCAATCCAAC 
AGCATATTTG GTTGCCTGTT CTACACCTTA CAGCTGTTGT TAGGTTGCTT GAGGGGACGT 
TGGGCCTCTA TCCTACTGGT GCTGAGTTCC CTGGTGTCCG TCGCTGGTTC CGTGTACCTG 
GCCTGGATCC TGTTCTTTGT GTTATATGAT TTCTGCATTG TGTGCATTAC CACCTATGCC 
ATCAATGTGG GTCTGATGTT GCTTAGCTTC CAGAAGGTAC CAGAACACAA GACCAAAAAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848715.1
CDS	37..522
Translation	MGTTWRSPGLVRLALCLAGLALSLYALHVKAARARDENYRALCDVGTAISCSRVFSSRWGRGFGLVEHMLGADSVLNQSNSIFGCLFYTLQLLLGCLRGRWASILLVLSSLVSVAGSVYLAWILFFVLYDFCIVCITTYAINVGLMLLSFQKVPEHKTKKH

Target ORF information:

RefSeq Version	NM_178600.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178600.2

ORF Insert Sequence:

ATGGGCACCA CCTGGAGGAG CCCTGGACTC GTGCGGCTTG CACTGTGCCT CGCTGGCTTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCGATGA AAATTACCGC 
GCGCTCTGCG ATGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTGGGGC 
CGGGGCTTTG GGCTGGTGGA GCACATGCTA GGAGCGGACA GCGTCCTCAA CCAATCCAAC 
AGCATATTTG GTTGCCTGTT CTACACCTTA CAGCTGTTGT TAGGTTGCTT GAGGGGACGT 
TGGGCCTCTA TCCTACTGGT GCTGAGTTCC CTGGTGTCCG TCGCTGGTTC CGTGTACCTG 
GCCTGGATCC TGTTCTTTGT GTTATATGAT TTCTGCATTG TGTGCATTAC CACCTATGCC 
ATCAATGTGG GTCTGATGTT GCTTAGCTTC CAGAAGGTAC CAGAACACAA GACCAAAAAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vkorc1 ( XM_030242648.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098508.1 Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89517
Clone ID Related Accession (Same CDS sequence)	XM_030242648.1
Accession Version	XM_030242648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vitamin K epoxide reductase complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCA CCTGGAGGAG CCCTGGACTC GTGCGGCTTG CACTGTGCCT CGCTGGCTTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCGATGA AAATTACCGC 
GCGCTCTGCG ATGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTTGCTT 
GAGGGGACGT TGGGCCTCTA TCCTACTGGT GCTGAGTTCC CTGGTGTCCG TCGCTGGTTC 
CGTGTACCTG GCCTGGATCC TGTTCTTTGT GTTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098508.1
CDS	78..356
Translation	MGTTWRSPGLVRLALCLAGLALSLYALHVKAARARDENYRALCDVGTAISCSRVFSSRLLEGTLGLYPTGAEFPGVRRWFRVPGLDPVLCVI

Target ORF information:

RefSeq Version	XM_030242648.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vitamin K epoxide reductase complex, subunit 1 (Vkorc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242648.1

ORF Insert Sequence:

ATGGGCACCA CCTGGAGGAG CCCTGGACTC GTGCGGCTTG CACTGTGCCT CGCTGGCTTA 
GCCCTCTCAC TGTACGCACT GCACGTGAAG GCGGCGCGCG CCCGCGATGA AAATTACCGC 
GCGCTCTGCG ATGTGGGCAC GGCCATCAGC TGTTCCCGCG TCTTCTCCTC TCGGTTGCTT 
GAGGGGACGT TGGGCCTCTA TCCTACTGGT GCTGAGTTCC CTGGTGTCCG TCGCTGGTTC 
CGTGTACCTG GCCTGGATCC TGTTCTTTGT GTTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
JCI insight3(1)(2018 Jan)
Lacombe J,Rishavy MA,Berkner KL,Ferron M

Two enzymes catalyze vitamin K 2,3-epoxide reductase activity in mouse: VKORC1 is highly expressed in exocrine tissues while VKORC1L1 is highly expressed in brain.
Thrombosis research135(5)977-83(2015 May)
Caspers M,Czogalla KJ,Liphardt K,M?ller J,Westhofen P,Watzka M,Oldenburg J

GGCX and VKORC1 inhibit osteocalcin endocrine functions.
The Journal of cell biology208(6)761-76(2015 Mar)
Ferron M,Lacombe J,Germain A,Oury F,Karsenty G

VKORC1L1, an enzyme rescuing the vitamin K 2,3-epoxide reductase activity in some extrahepatic tissues during anticoagulation therapy.
The Journal of biological chemistry288(40)28733-42(2013 Oct)
Hammed A,Matagrin B,Spohn G,Prouillac C,Benoit E,Lattard V

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Vkorc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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