Nfat5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfat5
Entrez Gene ID	307820
Full Name	nuclear factor of activated T-cells 5
Synonyms	NF-AT5,Nfat,TonEBP
General protein information	Preferred Names nuclear factor of activated T-cells 5 Names nuclear factor of activated T-cells 5 T-cell transcription factor NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255555.3	XP_006255617.1	nuclear factor of activated T-cells 5 isoform X5
XM_008772503.2	XP_008770725.1	nuclear factor of activated T-cells 5 isoform X1
XM_003751872.4	XP_003751920.1	nuclear factor of activated T-cells 5 isoform X2
NM_001107425.1	NP_001100895.1	nuclear factor of activated T-cells 5
XM_008772504.2	XP_008770726.1	nuclear factor of activated T-cells 5 isoform X3
XM_017601291.1	XP_017456780.1	nuclear factor of activated T-cells 5 isoform X4
XM_008772505.2	XP_008770727.1	nuclear factor of activated T-cells 5 isoform X5
XM_006255556.3	XP_006255618.1	nuclear factor of activated T-cells 5 isoform X5

Pathways from BioSystems

Homologies

	NFAT5	XP_001093880.2
Mus musculus (house mouse)	Nfat5	NP_061293.2
Xenopus tropicalis (tropical clawed frog)	nfat5	XP_002937623.2
Homo sapiens (human)	NFAT5	NP_619727.2
Pan troglodytes (chimpanzee)	NFAT5	XP_001168930.2
Canis lupus familiaris (dog)	NFAT5	XP_005620824.1
Gallus gallus (chicken)	NFAT5	NP_001185929.1
Bos taurus (cattle)	NFAT5	XP_002694885.1
Rattus norvegicus (Norway rat)	Nfat5	NP_001100895.1
Danio rerio (zebrafish)	LOC555802	XP_005174610.1

Gene Ontology
Bibliography

Related articles in PubMed

TNF-? promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells.
Johnson ZI, Doolittle AC, Snuggs JW, Shapiro IM, Le Maitre CL, Risbud MV
The Journal of biological chemistry292(42)17561-17575(2017 10)

Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus.
Liu C, Choi H, Johnson ZI, Tian J, Shapiro IM, Risbud MV
Scientific reports7(1)4543(2017 Jul)

NFAT5 protects astrocytes against oxygen-glucose-serum deprivation/restoration damage via the SIRT1/Nrf2 pathway.
Xia X, Qu B, Li YM, Yang LB, Fan KX, Zheng H, Huang HD, Gu JW, Kuang YQ, Ma Y
Journal of molecular neuroscience : MN61(1)96-104(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TonEBP expression correlated with canonical osmoregulatory targets TauT/SLC6A6, SMIT/SLC5A3, and AR/AKR1B1, supporting in vitro findings that the inflammatory milieu during IDD does not interfere with TonEBP osmoregulation. In summary, whereas TonEBP participates in the proinflammatory response to TNF-alpha
Title: TNF-? promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells.

Overexpression of NFAT5 promoted survival of the astrocytes and SIRT1 activation under oxygen-glucose-serum deprivation/restoration damage and inhibited cell apoptosis.
Title: NFAT5 protects astrocytes against oxygen-glucose-serum deprivation/restoration damage via the SIRT1/Nrf2 pathway.

NFAT5 serves an important pathophysiological role in seawater inhalationinduced acute lung injury by modulating NFkappaB activity.
Title: NFAT5 participates in seawater inhalation?induced acute lung injury via modulation of NF-?B activity.

Together these results show that the expression of TonEBP is regulated by inflammatory signals in mammalian brain, suggesting that TonEBP might play a part during neuroinflammation.
Title: Inflammatory signals induce the expression of tonicity-responsive enhancer binding protein (TonEBP) in microglia.

Therefore, we suggest that there is a regional heterogeneity of regulation of renal NPRs, TonEBP, and APQ-2 mRNA in acute kidney injury .
Title: Regional heterogeneity of expression of renal NPRs, TonEBP, and AQP-2 mRNAs in rats with acute kidney injury.

The following Nfat5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfat5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42696	NM_001107425.1 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5 (Nfat5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1140.30 $1629.00
ORa38897	XM_003751872.4 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa38898	XM_017601291.1 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa38899	XM_008772505.2 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa38899	XM_006255556.3 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa38899	XM_006255555.3 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa46552	XM_008772503.2 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa46553	XM_008772504.2 Latest version!	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfat5 ( NM_001107425.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100895.1 Rattus norvegicus nuclear factor of activated T-cells 5 (Nfat5), mRNA.

Price & Availability↑

CloneID	ORa42696
Clone ID Related Accession (Same CDS sequence)	NM_001107425.1
Accession Version	NM_001107425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Dec 15, 2013 this sequence version replaced XM_003751870.2, XM_001076581.1, XM_226436.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ACCTAGAGAA 
ACATCTGCAG CATCAATGAG TCAGACAAGC GGTGGCGAGG CAGGCTCGCC TCCTCCAGCT 
GTAGTTGCTG CCGATGCTTC TTCAGCTCCC TCCTCCTCCA TGGGCGGTGC TTGCAGCTCC 
TTTACCACCT CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG 
CAAGTGGAGA GCTGCTCCTC AGCCGTGGGG GTAAGTAACA GAGGGGTAAG TGAAAAGCAG 
TTAACCGGTA ACACAGTTCA GCAGCATCCA TCAACCCCGA AGAGGCACAC AGTTTTGTAC 
ATCTCACCAC CACCTGAGGA CTTGCTGGAT AACAGTCGGA TGTCCTGCCA GGATGAGGGG 
TGTGGATTGG AATCTGAGCA GAGCTGCAGT ATGTGGATGG AGGATTCCCC CTCCAACTTC 
AGTAACATGA GCACCAGTTC CTACAATGAT AACACTGAGG TACCTCGTAA ATCACGAAAA 
CGAAATCCAA AGCAGAGGCC GGGGGTCAAA CGACGAGATT GTGAAGAATC TAATATGGAT 
ATATTTGATG CCGACAGTGC CAAAGCACCT CACTATGTGC TTTCTCAGCT TACCACGGAC 
AACAAAGGCA ACTCAAAAGC TGGAAATGGA ACATTGGACA GCCAAAAGGG AACTGGAGTA 
AAGAAGAGTC CTATGTTGTG TGGACAGTAT CCGGTTAAAA GTGAGGGGAA GGAGCTGAAG 
ATAGTGGTAC AGCCTGAAAC CCAACACCGA GCCCGGTACC TGACAGAGGG CAGCCGAGGC 
TCCGTAAAAG ATAGAACACA GCAAGGCTTT CCTACGGTGA AGCTGGAAGG TCATAATGAG 
CCAGTGGTGT TGCAGGTGTT TGTGGGCAAT GACTCTGGTC GAGTAAAGCC ACACGGATTC 
TATCAGGCCT GCAGAGTGAC CGGGCGAAAC ACAACTCCGT GCAAAGAAGT GGACATCGAA 
GGCACCACTG TGATAGAGGT TGGCCTCGAC CCTAGCAGCA ACATGACGCT GGCAGTGGAC 
TGTGTAGGAA TACTGAAGCT GAGAAATGCT GATGTTGAGG CCAGAATTGG AATTGCTGGT 
TCTAAGAAAA AGAGCACTCG TGCCAGATTG GTTTTTCGAG TTAATATCAC GAGGAAAGAT 
GGCTCTACTC TGACATTGCA GACACCTTCT TCCCCAATTT TATGCACTCA GCCAGCAGGA 
GTTCCTGAGA TCTTAAAGAA AAGCTTGCAT AGCTGTTCAG TGAAAGGAGA GGAAGAAGTG 
TTTTTAATTG GGAAGAACTT TCTGAAAGGA ACTAAAGTTA TTTTCCAGGA AAATGTTTCT 
GATGAAAACT CTTGGAAATC AGAAGCTGAA ATTGACATGG AATTGTTCCA TCAGAACCAT 
CTTATTGTGA AAGTTCCTCC ATATCATGAC CAACATATAA CTTTGCCTGT ATCAGTGGGA 
ATATATGTTG TGACCAATGC TGGAAGATCT CATGATGTTC AGCCATTTAC TTACACTCCA 
GATCCAGCAG CTGGTGCTTT GAGTGTAAAT GTGAAAAAGG AGATATCTAG TCCAGCAAGA 
CCTTGCTCTT TTGAAGAAGC CTTGAAAGCA ATGAAAACGA CTGGCTGTAA CGTAGATAAG 
GTAACTATCC TTCCTAATGC CCTGATCACT CCACTCATAT CGAGCAGCAT GATTAAGACT 
GAAGATGTTA CTCCAATGGA AGTAACATCA GAGAAAAGAT CTTCCCCAAT TTTTCAGACT 
ACAAAGACAG TCGGATCAAC CCAGCAAACT TTAGAAACTA TTTCTAACAT AGCGGGAAAT 
GCATCCTTTT CATCACCATC ATCTTCTCAC TTATCTCCTG AAAATGAAAA TCAGCAGCAG 
CTTCAGCCCA AGGCATACAA CCCGGAGACT CTGACAACTA TCCAAACGCA AGACATCTCA 
CAGCCAGGGA CTTTTCCAGC GGTTTCTGCT TCTAGTCAGC TGCCCAGCAG CGATGCACTG 
CTGCAGCAGG CGGCACAGTT TCAGACAAGA GACGCTCAGT CCAGAGACAC AATGCAGTCA 
GACTCAGTGG TTAACTTGTC ACAGTTGACT GAGGCACCAC AGCAGCAGCA GTCCCCACTG 
CAAGAACAAG CACAGATACC ATCAAATATT TTTCCGTCGC CAAATAGTGT GAGCCAGCTC 
CAGAGTACTA TTCAGCAGCT GCAAGCAGGA AGTTTTACAG GCAGCACTGC TAGTGGCAGC 
AATGGAAGTG TTGACTTGGT CCAGCAGGTT TTAGAGGCAC AACAACAGTT ATCGTCTGTT 
CTGTTTTCTA CTCCAGATGG TAATGAGAAT GTTCAAGAAC AGCTGAATGC AGACATTTTC 
CAAGTCAGTC AAATTCAAAA TAGTGTAAGC CCTGGAATGT TTTCCTCAAC AGAGTCTGCA 
GTTCACACCA GACCAGATAA CTTACTACCT GGGAGGGCCG ACAGCGTCCA TCAGCAGACT 
GAAAACACAC TGTCTAGTCA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAAGTGATAG AGTCTTCAGC TGCCATGGTG ATGGAGATGC AGCAGAGCAT TTGCCAAGCA 
GCAGCCCAGA TTCAGTCAGA ACTGTTTCCT TCAGCTGCTT CAGCAAATGG AAGCCTTCAG 
CAGTCTCCAG TTTACCAGCA GCCTTCTCAC ATGATGAGCG CCCTGCCTAC CAGCGAGGAC 
ATGCAAATGC AGTGTGAATT GTTCTCTTCC CCCCCTGCAG TTTCTGGAAA TGAAACATCC 
ACCACCACCA CACCGCAGGT GGCAACCCCC GGCTCTACTA TGTTTCAGCC GCCAAATTCA 
GGCGATGGAG AAGAAACGGG AGCACAAGCA AAACAGATTC AGAGCAGTGT CTTTCAGACA 
ATGGTCCAGA TGCAGCACAG TGGGGACAGC CAACCTCAAG TAAACCTCTT TTCATCTACA 
AAAAACATAA TGAGTGTTCA GAGTAATGGT ACCCAGCAAC AAGGGAATAG CTTGTTCCAG 
CAGGGGAGCG AGATGCTGTC ACTTCAGTCT GGGAGCTTCT TGCAGCAGTC TTCTCATTCC 
CAGGCTCAGC TCTTTCACCC TCAGAACCCT ATTGCTGATG CTCAGAGCCT TTCCCAGGAG 
ACTCAAGGTC CAATGTTTCA TAGCGCCAAT CCCATCGTCC ACAGTCAGAC TTCCACAGCA 
TCCTCCGAAC AGCTGCAGCC TTCCATGTTT CACTCGCAGA GTACCATTGC TGTACTACAG 
GGCTCTTCAG TCCCTCAGGA CCAGCAGTCA CCCAACATAT ATCTTTCCCA AAGTTCCATC 
AGTAATCTTC AAACTAACAC AGTAGCCCAA GAAGAGCAGA TTTCATTTTT TTCAGCACAG 
AACTCAATTT CTCCACTTCA GTCAACATCA AACACTGAGC AGCAAGCTGC TTTTCAACAG 
CAGCCTCCAA TCTCGCACAT CCAGACCCCT CTTCTTTCCC AGGAACAGGC ACAGCCCTCT 
CAGCAAGGTT TATTTCAGCC TCAGGTGGCC CTGGGCTCCC TTCCTGCTAA TCCAATGCCT 
CAGAACCAGC AAGGCCCAAT TTTCCAGACA CAGCGCCCAA TAGTTGGCAT GCAGAGTAAC 
TCTCCATCCC AGGAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCAGA GCATTTTATT TAGTAATCAG AATGCCATGG CTACAATGGC CTCCCAGAAG 
CAGCCACCAC CAAACATGAT ATTTAGCCCA AACCAGAACC CGATGGCTAG TCAAGAGCAG 
CAGAACCAGT CAATTTTTCA CCAGCAAAGT AATATGGCCC CAATGAACCA AGAGCAGCAG 
CCCATGCAGT TTCAGAATCA GCCTACGGTT TCCTCACTTC AGAACCCAGG TCCTACGCCA 
TCTGAGTCAC CACAGACCTC CTTGTTCCAT AGTTCTCCTC AGATCCAGTT GGTTCAAGGG 
TCACCTAGTT CTCAAGAGCA ACAAGTAACA CTCTTCCTCT CTCCAGCATC CATGTCTGCA 
TTGCAAACCA GTATAAACCA ACCAGACATG CAGCAGTCTC CTCTTTATTC CCCACAGAAC 
AACATCCCTG GAATCCAAGG AAGCACTTCT TCACCCCAGC CACAGGCTGC TTTATTTCAC 
AACACTACAG GAGGCACAAT AAACCAACTA CAAAATTCTC CTGGCTCATC TCAGCAGACT 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AATTGTAGTC AGCTGTTAAC TTCTGGGCCA 
GCTACATTGC CAGAGCAGCT GATGGCCATA AATCAGCCGG GCCAACCACA AAATGAGGGC 
CAATCTTCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AATCTGCCCC ACTGGCCTCC 
TCGGTGAACA ACAGTCAGAA CATGGAAAAG ATCGATTTGC TTGTTTCGTT GCAAAGCCAA 
GGGAACAATT TAACCGGCTC CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100895.1
CDS	807..5453
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPPRETSAASMSQTSGGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	NM_001107425.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5 (Nfat5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107425.1

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ACCTAGAGAA 
ACATCTGCAG CATCAATGAG TCAGACAAGC GGTGGCGAGG CAGGCTCGCC TCCTCCAGCT 
GTAGTTGCTG CCGATGCTTC TTCAGCTCCC TCCTCCTCCA TGGGCGGTGC TTGCAGCTCC 
TTTACCACCT CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG 
CAAGTGGAGA GCTGCTCCTC AGCCGTGGGG GTAAGTAACA GAGGGGTAAG TGAAAAGCAG 
TTAACCGGTA ACACAGTTCA GCAGCATCCA TCAACCCCGA AGAGGCACAC AGTTTTGTAC 
ATCTCACCAC CACCTGAGGA CTTGCTGGAT AACAGTCGGA TGTCCTGCCA GGATGAGGGG 
TGTGGATTGG AATCTGAGCA GAGCTGCAGT ATGTGGATGG AGGATTCCCC CTCCAACTTC 
AGTAACATGA GCACCAGTTC CTACAATGAT AACACTGAGG TACCTCGTAA ATCACGAAAA 
CGAAATCCAA AGCAGAGGCC GGGGGTCAAA CGACGAGATT GTGAAGAATC TAATATGGAT 
ATATTTGATG CCGACAGTGC CAAAGCACCT CACTATGTGC TTTCTCAGCT TACCACGGAC 
AACAAAGGCA ACTCAAAAGC TGGAAATGGA ACATTGGACA GCCAAAAGGG AACTGGAGTA 
AAGAAGAGTC CTATGTTGTG TGGACAGTAT CCGGTTAAAA GTGAGGGGAA GGAGCTGAAG 
ATAGTGGTAC AGCCTGAAAC CCAACACCGA GCCCGGTACC TGACAGAGGG CAGCCGAGGC 
TCCGTAAAAG ATAGAACACA GCAAGGCTTT CCTACGGTGA AGCTGGAAGG TCATAATGAG 
CCAGTGGTGT TGCAGGTGTT TGTGGGCAAT GACTCTGGTC GAGTAAAGCC ACACGGATTC 
TATCAGGCCT GCAGAGTGAC CGGGCGAAAC ACAACTCCGT GCAAAGAAGT GGACATCGAA 
GGCACCACTG TGATAGAGGT TGGCCTCGAC CCTAGCAGCA ACATGACGCT GGCAGTGGAC 
TGTGTAGGAA TACTGAAGCT GAGAAATGCT GATGTTGAGG CCAGAATTGG AATTGCTGGT 
TCTAAGAAAA AGAGCACTCG TGCCAGATTG GTTTTTCGAG TTAATATCAC GAGGAAAGAT 
GGCTCTACTC TGACATTGCA GACACCTTCT TCCCCAATTT TATGCACTCA GCCAGCAGGA 
GTTCCTGAGA TCTTAAAGAA AAGCTTGCAT AGCTGTTCAG TGAAAGGAGA GGAAGAAGTG 
TTTTTAATTG GGAAGAACTT TCTGAAAGGA ACTAAAGTTA TTTTCCAGGA AAATGTTTCT 
GATGAAAACT CTTGGAAATC AGAAGCTGAA ATTGACATGG AATTGTTCCA TCAGAACCAT 
CTTATTGTGA AAGTTCCTCC ATATCATGAC CAACATATAA CTTTGCCTGT ATCAGTGGGA 
ATATATGTTG TGACCAATGC TGGAAGATCT CATGATGTTC AGCCATTTAC TTACACTCCA 
GATCCAGCAG CTGGTGCTTT GAGTGTAAAT GTGAAAAAGG AGATATCTAG TCCAGCAAGA 
CCTTGCTCTT TTGAAGAAGC CTTGAAAGCA ATGAAAACGA CTGGCTGTAA CGTAGATAAG 
GTAACTATCC TTCCTAATGC CCTGATCACT CCACTCATAT CGAGCAGCAT GATTAAGACT 
GAAGATGTTA CTCCAATGGA AGTAACATCA GAGAAAAGAT CTTCCCCAAT TTTTCAGACT 
ACAAAGACAG TCGGATCAAC CCAGCAAACT TTAGAAACTA TTTCTAACAT AGCGGGAAAT 
GCATCCTTTT CATCACCATC ATCTTCTCAC TTATCTCCTG AAAATGAAAA TCAGCAGCAG 
CTTCAGCCCA AGGCATACAA CCCGGAGACT CTGACAACTA TCCAAACGCA AGACATCTCA 
CAGCCAGGGA CTTTTCCAGC GGTTTCTGCT TCTAGTCAGC TGCCCAGCAG CGATGCACTG 
CTGCAGCAGG CGGCACAGTT TCAGACAAGA GACGCTCAGT CCAGAGACAC AATGCAGTCA 
GACTCAGTGG TTAACTTGTC ACAGTTGACT GAGGCACCAC AGCAGCAGCA GTCCCCACTG 
CAAGAACAAG CACAGATACC ATCAAATATT TTTCCGTCGC CAAATAGTGT GAGCCAGCTC 
CAGAGTACTA TTCAGCAGCT GCAAGCAGGA AGTTTTACAG GCAGCACTGC TAGTGGCAGC 
AATGGAAGTG TTGACTTGGT CCAGCAGGTT TTAGAGGCAC AACAACAGTT ATCGTCTGTT 
CTGTTTTCTA CTCCAGATGG TAATGAGAAT GTTCAAGAAC AGCTGAATGC AGACATTTTC 
CAAGTCAGTC AAATTCAAAA TAGTGTAAGC CCTGGAATGT TTTCCTCAAC AGAGTCTGCA 
GTTCACACCA GACCAGATAA CTTACTACCT GGGAGGGCCG ACAGCGTCCA TCAGCAGACT 
GAAAACACAC TGTCTAGTCA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAAGTGATAG AGTCTTCAGC TGCCATGGTG ATGGAGATGC AGCAGAGCAT TTGCCAAGCA 
GCAGCCCAGA TTCAGTCAGA ACTGTTTCCT TCAGCTGCTT CAGCAAATGG AAGCCTTCAG 
CAGTCTCCAG TTTACCAGCA GCCTTCTCAC ATGATGAGCG CCCTGCCTAC CAGCGAGGAC 
ATGCAAATGC AGTGTGAATT GTTCTCTTCC CCCCCTGCAG TTTCTGGAAA TGAAACATCC 
ACCACCACCA CACCGCAGGT GGCAACCCCC GGCTCTACTA TGTTTCAGCC GCCAAATTCA 
GGCGATGGAG AAGAAACGGG AGCACAAGCA AAACAGATTC AGAGCAGTGT CTTTCAGACA 
ATGGTCCAGA TGCAGCACAG TGGGGACAGC CAACCTCAAG TAAACCTCTT TTCATCTACA 
AAAAACATAA TGAGTGTTCA GAGTAATGGT ACCCAGCAAC AAGGGAATAG CTTGTTCCAG 
CAGGGGAGCG AGATGCTGTC ACTTCAGTCT GGGAGCTTCT TGCAGCAGTC TTCTCATTCC 
CAGGCTCAGC TCTTTCACCC TCAGAACCCT ATTGCTGATG CTCAGAGCCT TTCCCAGGAG 
ACTCAAGGTC CAATGTTTCA TAGCGCCAAT CCCATCGTCC ACAGTCAGAC TTCCACAGCA 
TCCTCCGAAC AGCTGCAGCC TTCCATGTTT CACTCGCAGA GTACCATTGC TGTACTACAG 
GGCTCTTCAG TCCCTCAGGA CCAGCAGTCA CCCAACATAT ATCTTTCCCA AAGTTCCATC 
AGTAATCTTC AAACTAACAC AGTAGCCCAA GAAGAGCAGA TTTCATTTTT TTCAGCACAG 
AACTCAATTT CTCCACTTCA GTCAACATCA AACACTGAGC AGCAAGCTGC TTTTCAACAG 
CAGCCTCCAA TCTCGCACAT CCAGACCCCT CTTCTTTCCC AGGAACAGGC ACAGCCCTCT 
CAGCAAGGTT TATTTCAGCC TCAGGTGGCC CTGGGCTCCC TTCCTGCTAA TCCAATGCCT 
CAGAACCAGC AAGGCCCAAT TTTCCAGACA CAGCGCCCAA TAGTTGGCAT GCAGAGTAAC 
TCTCCATCCC AGGAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCAGA GCATTTTATT TAGTAATCAG AATGCCATGG CTACAATGGC CTCCCAGAAG 
CAGCCACCAC CAAACATGAT ATTTAGCCCA AACCAGAACC CGATGGCTAG TCAAGAGCAG 
CAGAACCAGT CAATTTTTCA CCAGCAAAGT AATATGGCCC CAATGAACCA AGAGCAGCAG 
CCCATGCAGT TTCAGAATCA GCCTACGGTT TCCTCACTTC AGAACCCAGG TCCTACGCCA 
TCTGAGTCAC CACAGACCTC CTTGTTCCAT AGTTCTCCTC AGATCCAGTT GGTTCAAGGG 
TCACCTAGTT CTCAAGAGCA ACAAGTAACA CTCTTCCTCT CTCCAGCATC CATGTCTGCA 
TTGCAAACCA GTATAAACCA ACCAGACATG CAGCAGTCTC CTCTTTATTC CCCACAGAAC 
AACATCCCTG GAATCCAAGG AAGCACTTCT TCACCCCAGC CACAGGCTGC TTTATTTCAC 
AACACTACAG GAGGCACAAT AAACCAACTA CAAAATTCTC CTGGCTCATC TCAGCAGACT 
TCAGGAATGT TCTTATTTGG CATTCAAAAT AATTGTAGTC AGCTGTTAAC TTCTGGGCCA 
GCTACATTGC CAGAGCAGCT GATGGCCATA AATCAGCCGG GCCAACCACA AAATGAGGGC 
CAATCTTCTG TGACAACACT TCTTTCTCAG CAAATGCCAG AATCTGCCCC ACTGGCCTCC 
TCGGTGAACA ACAGTCAGAA CATGGAAAAG ATCGATTTGC TTGTTTCGTT GCAAAGCCAA 
GGGAACAATT TAACCGGCTC CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_003751872.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003751920.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38897
Clone ID Related Accession (Same CDS sequence)	XM_003751872.4
Accession Version	XM_003751872.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003751872.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCACCT 
AGAGAAACAT CTGCAGCATC AATGAGTCAG ACAAGCGGTG GCGAGGCAGG CTCGCCTCCT 
CCAGCTGTAG TTGCTGCCGA TGCTTCTTCA GCTCCCTCCT CCTCCATGGG CGGTGCTTGC 
AGCTCCTTTA CCACCTCTTC CAGCCCTACC ATTTATTCTA CCTCAGTCAC CGACAGCAAG 
GCTATGCAAG TGGAGAGCTG CTCCTCAGCC GTGGGGGTAA GTAACAGAGG GGTAAGTGAA 
AAGCAGTTAA CCGGTAACAC AGTTCAGCAG CATCCATCAA CCCCGAAGAG GCACACAGTT 
TTGTACATCT CACCACCACC TGAGGACTTG CTGGATAACA GTCGGATGTC CTGCCAGGAT 
GAGGGGTGTG GATTGGAATC TGAGCAGAGC TGCAGTATGT GGATGGAGGA TTCCCCCTCC 
AACTTCAGTA ACATGAGCAC CAGTTCCTAC AATGATAACA CTGAGGTACC TCGTAAATCA 
CGAAAACGAA ATCCAAAGCA GAGGCCGGGG GTCAAACGAC GAGATTGTGA AGAATCTAAT 
ATGGATATAT TTGATGCCGA CAGTGCCAAA GCACCTCACT ATGTGCTTTC TCAGCTTACC 
ACGGACAACA AAGGCAACTC AAAAGCTGGA AATGGAACAT TGGACAGCCA AAAGGGAACT 
GGAGTAAAGA AGAGTCCTAT GTTGTGTGGA CAGTATCCGG TTAAAAGTGA GGGGAAGGAG 
CTGAAGATAG TGGTACAGCC TGAAACCCAA CACCGAGCCC GGTACCTGAC AGAGGGCAGC 
CGAGGCTCCG TAAAAGATAG AACACAGCAA GGCTTTCCTA CGGTGAAGCT GGAAGGTCAT 
AATGAGCCAG TGGTGTTGCA GGTGTTTGTG GGCAATGACT CTGGTCGAGT AAAGCCACAC 
GGATTCTATC AGGCCTGCAG AGTGACCGGG CGAAACACAA CTCCGTGCAA AGAAGTGGAC 
ATCGAAGGCA CCACTGTGAT AGAGGTTGGC CTCGACCCTA GCAGCAACAT GACGCTGGCA 
GTGGACTGTG TAGGAATACT GAAGCTGAGA AATGCTGATG TTGAGGCCAG AATTGGAATT 
GCTGGTTCTA AGAAAAAGAG CACTCGTGCC AGATTGGTTT TTCGAGTTAA TATCACGAGG 
AAAGATGGCT CTACTCTGAC ATTGCAGACA CCTTCTTCCC CAATTTTATG CACTCAGCCA 
GCAGGAGTTC CTGAGATCTT AAAGAAAAGC TTGCATAGCT GTTCAGTGAA AGGAGAGGAA 
GAAGTGTTTT TAATTGGGAA GAACTTTCTG AAAGGAACTA AAGTTATTTT CCAGGAAAAT 
GTTTCTGATG AAAACTCTTG GAAATCAGAA GCTGAAATTG ACATGGAATT GTTCCATCAG 
AACCATCTTA TTGTGAAAGT TCCTCCATAT CATGACCAAC ATATAACTTT GCCTGTATCA 
GTGGGAATAT ATGTTGTGAC CAATGCTGGA AGATCTCATG ATGTTCAGCC ATTTACTTAC 
ACTCCAGATC CAGCAGCTGG TGCTTTGAGT GTAAATGTGA AAAAGGAGAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAAGCCTTG AAAGCAATGA AAACGACTGG CTGTAACGTA 
GATAAGGTAA CTATCCTTCC TAATGCCCTG ATCACTCCAC TCATATCGAG CAGCATGATT 
AAGACTGAAG ATGTTACTCC AATGGAAGTA ACATCAGAGA AAAGATCTTC CCCAATTTTT 
CAGACTACAA AGACAGTCGG ATCAACCCAG CAAACTTTAG AAACTATTTC TAACATAGCG 
GGAAATGCAT CCTTTTCATC ACCATCATCT TCTCACTTAT CTCCTGAAAA TGAAAATCAG 
CAGCAGCTTC AGCCCAAGGC ATACAACCCG GAGACTCTGA CAACTATCCA AACGCAAGAC 
ATCTCACAGC CAGGGACTTT TCCAGCGGTT TCTGCTTCTA GTCAGCTGCC CAGCAGCGAT 
GCACTGCTGC AGCAGGCGGC ACAGTTTCAG ACAAGAGACG CTCAGTCCAG AGACACAATG 
CAGTCAGACT CAGTGGTTAA CTTGTCACAG TTGACTGAGG CACCACAGCA GCAGCAGTCC 
CCACTGCAAG AACAAGCACA GATACCATCA AATATTTTTC CGTCGCCAAA TAGTGTGAGC 
CAGCTCCAGA GTACTATTCA GCAGCTGCAA GCAGGAAGTT TTACAGGCAG CACTGCTAGT 
GGCAGCAATG GAAGTGTTGA CTTGGTCCAG CAGGTTTTAG AGGCACAACA ACAGTTATCG 
TCTGTTCTGT TTTCTACTCC AGATGGTAAT GAGAATGTTC AAGAACAGCT GAATGCAGAC 
ATTTTCCAAG TCAGTCAAAT TCAAAATAGT GTAAGCCCTG GAATGTTTTC CTCAACAGAG 
TCTGCAGTTC ACACCAGACC AGATAACTTA CTACCTGGGA GGGCCGACAG CGTCCATCAG 
CAGACTGAAA ACACACTGTC TAGTCAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAG TGATAGAGTC TTCAGCTGCC ATGGTGATGG AGATGCAGCA GAGCATTTGC 
CAAGCAGCAG CCCAGATTCA GTCAGAACTG TTTCCTTCAG CTGCTTCAGC AAATGGAAGC 
CTTCAGCAGT CTCCAGTTTA CCAGCAGCCT TCTCACATGA TGAGCGCCCT GCCTACCAGC 
GAGGACATGC AAATGCAGTG TGAATTGTTC TCTTCCCCCC CTGCAGTTTC TGGAAATGAA 
ACATCCACCA CCACCACACC GCAGGTGGCA ACCCCCGGCT CTACTATGTT TCAGCCGCCA 
AATTCAGGCG ATGGAGAAGA AACGGGAGCA CAAGCAAAAC AGATTCAGAG CAGTGTCTTT 
CAGACAATGG TCCAGATGCA GCACAGTGGG GACAGCCAAC CTCAAGTAAA CCTCTTTTCA 
TCTACAAAAA ACATAATGAG TGTTCAGAGT AATGGTACCC AGCAACAAGG GAATAGCTTG 
TTCCAGCAGG GGAGCGAGAT GCTGTCACTT CAGTCTGGGA GCTTCTTGCA GCAGTCTTCT 
CATTCCCAGG CTCAGCTCTT TCACCCTCAG AACCCTATTG CTGATGCTCA GAGCCTTTCC 
CAGGAGACTC AAGGTCCAAT GTTTCATAGC GCCAATCCCA TCGTCCACAG TCAGACTTCC 
ACAGCATCCT CCGAACAGCT GCAGCCTTCC ATGTTTCACT CGCAGAGTAC CATTGCTGTA 
CTACAGGGCT CTTCAGTCCC TCAGGACCAG CAGTCACCCA ACATATATCT TTCCCAAAGT 
TCCATCAGTA ATCTTCAAAC TAACACAGTA GCCCAAGAAG AGCAGATTTC ATTTTTTTCA 
GCACAGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTT 
CAACAGCAGC CTCCAATCTC GCACATCCAG ACCCCTCTTC TTTCCCAGGA ACAGGCACAG 
CCCTCTCAGC AAGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC TGCTAATCCA 
ATGCCTCAGA ACCAGCAAGG CCCAATTTTC CAGACACAGC GCCCAATAGT TGGCATGCAG 
AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAGC AGCAGCAACA ACAACAGCAG 
CAGCAGCAGC AGCAGAGCAT TTTATTTAGT AATCAGAATG CCATGGCTAC AATGGCCTCC 
CAGAAGCAGC CACCACCAAA CATGATATTT AGCCCAAACC AGAACCCGAT GGCTAGTCAA 
GAGCAGCAGA ACCAGTCAAT TTTTCACCAG CAAAGTAATA TGGCCCCAAT GAACCAAGAG 
CAGCAGCCCA TGCAGTTTCA GAATCAGCCT ACGGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACGCCATCTG AGTCACCACA GACCTCCTTG TTCCATAGTT CTCCTCAGAT CCAGTTGGTT 
CAAGGGTCAC CTAGTTCTCA AGAGCAACAA GTAACACTCT TCCTCTCTCC AGCATCCATG 
TCTGCATTGC AAACCAGTAT AAACCAACCA GACATGCAGC AGTCTCCTCT TTATTCCCCA 
CAGAACAACA TCCCTGGAAT CCAAGGAAGC ACTTCTTCAC CCCAGCCACA GGCTGCTTTA 
TTTCACAACA CTACAGGAGG CACAATAAAC CAACTACAAA ATTCTCCTGG CTCATCTCAG 
CAGACTTCAG GAATGTTCTT ATTTGGCATT CAAAATAATT GTAGTCAGCT GTTAACTTCT 
GGGCCAGCTA CATTGCCAGA GCAGCTGATG GCCATAAATC AGCCGGGCCA ACCACAAAAT 
GAGGGCCAAT CTTCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAATC TGCCCCACTG 
GCCTCCTCGG TGAACAACAG TCAGAACATG GAAAAGATCG ATTTGCTTGT TTCGTTGCAA 
AGCCAAGGGA ACAATTTAAC CGGCTCCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003751920.1
CDS	792..5384
Translation	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPPRETSAASMSQTSGGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_003751872.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003751872.4

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCACCT 
AGAGAAACAT CTGCAGCATC AATGAGTCAG ACAAGCGGTG GCGAGGCAGG CTCGCCTCCT 
CCAGCTGTAG TTGCTGCCGA TGCTTCTTCA GCTCCCTCCT CCTCCATGGG CGGTGCTTGC 
AGCTCCTTTA CCACCTCTTC CAGCCCTACC ATTTATTCTA CCTCAGTCAC CGACAGCAAG 
GCTATGCAAG TGGAGAGCTG CTCCTCAGCC GTGGGGGTAA GTAACAGAGG GGTAAGTGAA 
AAGCAGTTAA CCGGTAACAC AGTTCAGCAG CATCCATCAA CCCCGAAGAG GCACACAGTT 
TTGTACATCT CACCACCACC TGAGGACTTG CTGGATAACA GTCGGATGTC CTGCCAGGAT 
GAGGGGTGTG GATTGGAATC TGAGCAGAGC TGCAGTATGT GGATGGAGGA TTCCCCCTCC 
AACTTCAGTA ACATGAGCAC CAGTTCCTAC AATGATAACA CTGAGGTACC TCGTAAATCA 
CGAAAACGAA ATCCAAAGCA GAGGCCGGGG GTCAAACGAC GAGATTGTGA AGAATCTAAT 
ATGGATATAT TTGATGCCGA CAGTGCCAAA GCACCTCACT ATGTGCTTTC TCAGCTTACC 
ACGGACAACA AAGGCAACTC AAAAGCTGGA AATGGAACAT TGGACAGCCA AAAGGGAACT 
GGAGTAAAGA AGAGTCCTAT GTTGTGTGGA CAGTATCCGG TTAAAAGTGA GGGGAAGGAG 
CTGAAGATAG TGGTACAGCC TGAAACCCAA CACCGAGCCC GGTACCTGAC AGAGGGCAGC 
CGAGGCTCCG TAAAAGATAG AACACAGCAA GGCTTTCCTA CGGTGAAGCT GGAAGGTCAT 
AATGAGCCAG TGGTGTTGCA GGTGTTTGTG GGCAATGACT CTGGTCGAGT AAAGCCACAC 
GGATTCTATC AGGCCTGCAG AGTGACCGGG CGAAACACAA CTCCGTGCAA AGAAGTGGAC 
ATCGAAGGCA CCACTGTGAT AGAGGTTGGC CTCGACCCTA GCAGCAACAT GACGCTGGCA 
GTGGACTGTG TAGGAATACT GAAGCTGAGA AATGCTGATG TTGAGGCCAG AATTGGAATT 
GCTGGTTCTA AGAAAAAGAG CACTCGTGCC AGATTGGTTT TTCGAGTTAA TATCACGAGG 
AAAGATGGCT CTACTCTGAC ATTGCAGACA CCTTCTTCCC CAATTTTATG CACTCAGCCA 
GCAGGAGTTC CTGAGATCTT AAAGAAAAGC TTGCATAGCT GTTCAGTGAA AGGAGAGGAA 
GAAGTGTTTT TAATTGGGAA GAACTTTCTG AAAGGAACTA AAGTTATTTT CCAGGAAAAT 
GTTTCTGATG AAAACTCTTG GAAATCAGAA GCTGAAATTG ACATGGAATT GTTCCATCAG 
AACCATCTTA TTGTGAAAGT TCCTCCATAT CATGACCAAC ATATAACTTT GCCTGTATCA 
GTGGGAATAT ATGTTGTGAC CAATGCTGGA AGATCTCATG ATGTTCAGCC ATTTACTTAC 
ACTCCAGATC CAGCAGCTGG TGCTTTGAGT GTAAATGTGA AAAAGGAGAT ATCTAGTCCA 
GCAAGACCTT GCTCTTTTGA AGAAGCCTTG AAAGCAATGA AAACGACTGG CTGTAACGTA 
GATAAGGTAA CTATCCTTCC TAATGCCCTG ATCACTCCAC TCATATCGAG CAGCATGATT 
AAGACTGAAG ATGTTACTCC AATGGAAGTA ACATCAGAGA AAAGATCTTC CCCAATTTTT 
CAGACTACAA AGACAGTCGG ATCAACCCAG CAAACTTTAG AAACTATTTC TAACATAGCG 
GGAAATGCAT CCTTTTCATC ACCATCATCT TCTCACTTAT CTCCTGAAAA TGAAAATCAG 
CAGCAGCTTC AGCCCAAGGC ATACAACCCG GAGACTCTGA CAACTATCCA AACGCAAGAC 
ATCTCACAGC CAGGGACTTT TCCAGCGGTT TCTGCTTCTA GTCAGCTGCC CAGCAGCGAT 
GCACTGCTGC AGCAGGCGGC ACAGTTTCAG ACAAGAGACG CTCAGTCCAG AGACACAATG 
CAGTCAGACT CAGTGGTTAA CTTGTCACAG TTGACTGAGG CACCACAGCA GCAGCAGTCC 
CCACTGCAAG AACAAGCACA GATACCATCA AATATTTTTC CGTCGCCAAA TAGTGTGAGC 
CAGCTCCAGA GTACTATTCA GCAGCTGCAA GCAGGAAGTT TTACAGGCAG CACTGCTAGT 
GGCAGCAATG GAAGTGTTGA CTTGGTCCAG CAGGTTTTAG AGGCACAACA ACAGTTATCG 
TCTGTTCTGT TTTCTACTCC AGATGGTAAT GAGAATGTTC AAGAACAGCT GAATGCAGAC 
ATTTTCCAAG TCAGTCAAAT TCAAAATAGT GTAAGCCCTG GAATGTTTTC CTCAACAGAG 
TCTGCAGTTC ACACCAGACC AGATAACTTA CTACCTGGGA GGGCCGACAG CGTCCATCAG 
CAGACTGAAA ACACACTGTC TAGTCAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAACAG 
CAGCAGCAAG TGATAGAGTC TTCAGCTGCC ATGGTGATGG AGATGCAGCA GAGCATTTGC 
CAAGCAGCAG CCCAGATTCA GTCAGAACTG TTTCCTTCAG CTGCTTCAGC AAATGGAAGC 
CTTCAGCAGT CTCCAGTTTA CCAGCAGCCT TCTCACATGA TGAGCGCCCT GCCTACCAGC 
GAGGACATGC AAATGCAGTG TGAATTGTTC TCTTCCCCCC CTGCAGTTTC TGGAAATGAA 
ACATCCACCA CCACCACACC GCAGGTGGCA ACCCCCGGCT CTACTATGTT TCAGCCGCCA 
AATTCAGGCG ATGGAGAAGA AACGGGAGCA CAAGCAAAAC AGATTCAGAG CAGTGTCTTT 
CAGACAATGG TCCAGATGCA GCACAGTGGG GACAGCCAAC CTCAAGTAAA CCTCTTTTCA 
TCTACAAAAA ACATAATGAG TGTTCAGAGT AATGGTACCC AGCAACAAGG GAATAGCTTG 
TTCCAGCAGG GGAGCGAGAT GCTGTCACTT CAGTCTGGGA GCTTCTTGCA GCAGTCTTCT 
CATTCCCAGG CTCAGCTCTT TCACCCTCAG AACCCTATTG CTGATGCTCA GAGCCTTTCC 
CAGGAGACTC AAGGTCCAAT GTTTCATAGC GCCAATCCCA TCGTCCACAG TCAGACTTCC 
ACAGCATCCT CCGAACAGCT GCAGCCTTCC ATGTTTCACT CGCAGAGTAC CATTGCTGTA 
CTACAGGGCT CTTCAGTCCC TCAGGACCAG CAGTCACCCA ACATATATCT TTCCCAAAGT 
TCCATCAGTA ATCTTCAAAC TAACACAGTA GCCCAAGAAG AGCAGATTTC ATTTTTTTCA 
GCACAGAACT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTT 
CAACAGCAGC CTCCAATCTC GCACATCCAG ACCCCTCTTC TTTCCCAGGA ACAGGCACAG 
CCCTCTCAGC AAGGTTTATT TCAGCCTCAG GTGGCCCTGG GCTCCCTTCC TGCTAATCCA 
ATGCCTCAGA ACCAGCAAGG CCCAATTTTC CAGACACAGC GCCCAATAGT TGGCATGCAG 
AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAGC AGCAGCAACA ACAACAGCAG 
CAGCAGCAGC AGCAGAGCAT TTTATTTAGT AATCAGAATG CCATGGCTAC AATGGCCTCC 
CAGAAGCAGC CACCACCAAA CATGATATTT AGCCCAAACC AGAACCCGAT GGCTAGTCAA 
GAGCAGCAGA ACCAGTCAAT TTTTCACCAG CAAAGTAATA TGGCCCCAAT GAACCAAGAG 
CAGCAGCCCA TGCAGTTTCA GAATCAGCCT ACGGTTTCCT CACTTCAGAA CCCAGGTCCT 
ACGCCATCTG AGTCACCACA GACCTCCTTG TTCCATAGTT CTCCTCAGAT CCAGTTGGTT 
CAAGGGTCAC CTAGTTCTCA AGAGCAACAA GTAACACTCT TCCTCTCTCC AGCATCCATG 
TCTGCATTGC AAACCAGTAT AAACCAACCA GACATGCAGC AGTCTCCTCT TTATTCCCCA 
CAGAACAACA TCCCTGGAAT CCAAGGAAGC ACTTCTTCAC CCCAGCCACA GGCTGCTTTA 
TTTCACAACA CTACAGGAGG CACAATAAAC CAACTACAAA ATTCTCCTGG CTCATCTCAG 
CAGACTTCAG GAATGTTCTT ATTTGGCATT CAAAATAATT GTAGTCAGCT GTTAACTTCT 
GGGCCAGCTA CATTGCCAGA GCAGCTGATG GCCATAAATC AGCCGGGCCA ACCACAAAAT 
GAGGGCCAAT CTTCTGTGAC AACACTTCTT TCTCAGCAAA TGCCAGAATC TGCCCCACTG 
GCCTCCTCGG TGAACAACAG TCAGAACATG GAAAAGATCG ATTTGCTTGT TTCGTTGCAA 
AGCCAAGGGA ACAATTTAAC CGGCTCCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_017601291.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456780.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa38898
Clone ID Related Accession (Same CDS sequence)	XM_017601291.1
Accession Version	XM_017601291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCGGT AACACAGTTC AGCAGCATCC ATCAACCCCG 
AAGAGGCACA CAGTTTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CTGGAAATGG AACATTGGAC 
AGCCAAAAGG GAACTGGAGT AAAGAAGAGT CCTATGTTGT GTGGACAGTA TCCGGTTAAA 
AGTGAGGGGA AGGAGCTGAA GATAGTGGTA CAGCCTGAAA CCCAACACCG AGCCCGGTAC 
CTGACAGAGG GCAGCCGAGG CTCCGTAAAA GATAGAACAC AGCAAGGCTT TCCTACGGTG 
AAGCTGGAAG GTCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTAAAGC CACACGGATT CTATCAGGCC TGCAGAGTGA CCGGGCGAAA CACAACTCCG 
TGCAAAGAAG TGGACATCGA AGGCACCACT GTGATAGAGG TTGGCCTCGA CCCTAGCAGC 
AACATGACGC TGGCAGTGGA CTGTGTAGGA ATACTGAAGC TGAGAAATGC TGATGTTGAG 
GCCAGAATTG GAATTGCTGG TTCTAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA CGAGGAAAGA TGGCTCTACT CTGACATTGC AGACACCTTC TTCCCCAATT 
TTATGCACTC AGCCAGCAGG AGTTCCTGAG ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT GTTTTTAATT GGGAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAGG AAAATGTTTC TGATGAAAAC TCTTGGAAAT CAGAAGCTGA AATTGACATG 
GAATTGTTCC ATCAGAACCA TCTTATTGTG AAAGTTCCTC CATATCATGA CCAACATATA 
ACTTTGCCTG TATCAGTGGG AATATATGTT GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTTA CTTACACTCC AGATCCAGCA GCTGGTGCTT TGAGTGTAAA TGTGAAAAAG 
GAGATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAAG CCTTGAAAGC AATGAAAACG 
ACTGGCTGTA ACGTAGATAA GGTAACTATC CTTCCTAATG CCCTGATCAC TCCACTCATA 
TCGAGCAGCA TGATTAAGAC TGAAGATGTT ACTCCAATGG AAGTAACATC AGAGAAAAGA 
TCTTCCCCAA TTTTTCAGAC TACAAAGACA GTCGGATCAA CCCAGCAAAC TTTAGAAACT 
ATTTCTAACA TAGCGGGAAA TGCATCCTTT TCATCACCAT CATCTTCTCA CTTATCTCCT 
GAAAATGAAA ATCAGCAGCA GCTTCAGCCC AAGGCATACA ACCCGGAGAC TCTGACAACT 
ATCCAAACGC AAGACATCTC ACAGCCAGGG ACTTTTCCAG CGGTTTCTGC TTCTAGTCAG 
CTGCCCAGCA GCGATGCACT GCTGCAGCAG GCGGCACAGT TTCAGACAAG AGACGCTCAG 
TCCAGAGACA CAATGCAGTC AGACTCAGTG GTTAACTTGT CACAGTTGAC TGAGGCACCA 
CAGCAGCAGC AGTCCCCACT GCAAGAACAA GCACAGATAC CATCAAATAT TTTTCCGTCG 
CCAAATAGTG TGAGCCAGCT CCAGAGTACT ATTCAGCAGC TGCAAGCAGG AAGTTTTACA 
GGCAGCACTG CTAGTGGCAG CAATGGAAGT GTTGACTTGG TCCAGCAGGT TTTAGAGGCA 
CAACAACAGT TATCGTCTGT TCTGTTTTCT ACTCCAGATG GTAATGAGAA TGTTCAAGAA 
CAGCTGAATG CAGACATTTT CCAAGTCAGT CAAATTCAAA ATAGTGTAAG CCCTGGAATG 
TTTTCCTCAA CAGAGTCTGC AGTTCACACC AGACCAGATA ACTTACTACC TGGGAGGGCC 
GACAGCGTCC ATCAGCAGAC TGAAAACACA CTGTCTAGTC AGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAGCA GCAAGTGATA GAGTCTTCAG CTGCCATGGT GATGGAGATG 
CAGCAGAGCA TTTGCCAAGC AGCAGCCCAG ATTCAGTCAG AACTGTTTCC TTCAGCTGCT 
TCAGCAAATG GAAGCCTTCA GCAGTCTCCA GTTTACCAGC AGCCTTCTCA CATGATGAGC 
GCCCTGCCTA CCAGCGAGGA CATGCAAATG CAGTGTGAAT TGTTCTCTTC CCCCCCTGCA 
GTTTCTGGAA ATGAAACATC CACCACCACC ACACCGCAGG TGGCAACCCC CGGCTCTACT 
ATGTTTCAGC CGCCAAATTC AGGCGATGGA GAAGAAACGG GAGCACAAGC AAAACAGATT 
CAGAGCAGTG TCTTTCAGAC AATGGTCCAG ATGCAGCACA GTGGGGACAG CCAACCTCAA 
GTAAACCTCT TTTCATCTAC AAAAAACATA ATGAGTGTTC AGAGTAATGG TACCCAGCAA 
CAAGGGAATA GCTTGTTCCA GCAGGGGAGC GAGATGCTGT CACTTCAGTC TGGGAGCTTC 
TTGCAGCAGT CTTCTCATTC CCAGGCTCAG CTCTTTCACC CTCAGAACCC TATTGCTGAT 
GCTCAGAGCC TTTCCCAGGA GACTCAAGGT CCAATGTTTC ATAGCGCCAA TCCCATCGTC 
CACAGTCAGA CTTCCACAGC ATCCTCCGAA CAGCTGCAGC CTTCCATGTT TCACTCGCAG 
AGTACCATTG CTGTACTACA GGGCTCTTCA GTCCCTCAGG ACCAGCAGTC ACCCAACATA 
TATCTTTCCC AAAGTTCCAT CAGTAATCTT CAAACTAACA CAGTAGCCCA AGAAGAGCAG 
ATTTCATTTT TTTCAGCACA GAACTCAATT TCTCCACTTC AGTCAACATC AAACACTGAG 
CAGCAAGCTG CTTTTCAACA GCAGCCTCCA ATCTCGCACA TCCAGACCCC TCTTCTTTCC 
CAGGAACAGG CACAGCCCTC TCAGCAAGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCTGCTA ATCCAATGCC TCAGAACCAG CAAGGCCCAA TTTTCCAGAC ACAGCGCCCA 
ATAGTTGGCA TGCAGAGTAA CTCTCCATCC CAGGAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGCAGCAGCA GCAGCAGCAG AGCATTTTAT TTAGTAATCA GAATGCCATG 
GCTACAATGG CCTCCCAGAA GCAGCCACCA CCAAACATGA TATTTAGCCC AAACCAGAAC 
CCGATGGCTA GTCAAGAGCA GCAGAACCAG TCAATTTTTC ACCAGCAAAG TAATATGGCC 
CCAATGAACC AAGAGCAGCA GCCCATGCAG TTTCAGAATC AGCCTACGGT TTCCTCACTT 
CAGAACCCAG GTCCTACGCC ATCTGAGTCA CCACAGACCT CCTTGTTCCA TAGTTCTCCT 
CAGATCCAGT TGGTTCAAGG GTCACCTAGT TCTCAAGAGC AACAAGTAAC ACTCTTCCTC 
TCTCCAGCAT CCATGTCTGC ATTGCAAACC AGTATAAACC AACCAGACAT GCAGCAGTCT 
CCTCTTTATT CCCCACAGAA CAACATCCCT GGAATCCAAG GAAGCACTTC TTCACCCCAG 
CCACAGGCTG CTTTATTTCA CAACACTACA GGAGGCACAA TAAACCAACT ACAAAATTCT 
CCTGGCTCAT CTCAGCAGAC TTCAGGAATG TTCTTATTTG GCATTCAAAA TAATTGTAGT 
CAGCTGTTAA CTTCTGGGCC AGCTACATTG CCAGAGCAGC TGATGGCCAT AAATCAGCCG 
GGCCAACCAC AAAATGAGGG CCAATCTTCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAATCTGCCC CACTGGCCTC CTCGGTGAAC AACAGTCAGA ACATGGAAAA GATCGATTTG 
CTTGTTTCGT TGCAAAGCCA AGGGAACAAT TTAACCGGCT CCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456780.1
CDS	370..4737
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_017601291.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601291.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCGGT AACACAGTTC AGCAGCATCC ATCAACCCCG 
AAGAGGCACA CAGTTTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CTGGAAATGG AACATTGGAC 
AGCCAAAAGG GAACTGGAGT AAAGAAGAGT CCTATGTTGT GTGGACAGTA TCCGGTTAAA 
AGTGAGGGGA AGGAGCTGAA GATAGTGGTA CAGCCTGAAA CCCAACACCG AGCCCGGTAC 
CTGACAGAGG GCAGCCGAGG CTCCGTAAAA GATAGAACAC AGCAAGGCTT TCCTACGGTG 
AAGCTGGAAG GTCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTAAAGC CACACGGATT CTATCAGGCC TGCAGAGTGA CCGGGCGAAA CACAACTCCG 
TGCAAAGAAG TGGACATCGA AGGCACCACT GTGATAGAGG TTGGCCTCGA CCCTAGCAGC 
AACATGACGC TGGCAGTGGA CTGTGTAGGA ATACTGAAGC TGAGAAATGC TGATGTTGAG 
GCCAGAATTG GAATTGCTGG TTCTAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA 
GTTAATATCA CGAGGAAAGA TGGCTCTACT CTGACATTGC AGACACCTTC TTCCCCAATT 
TTATGCACTC AGCCAGCAGG AGTTCCTGAG ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT GTTTTTAATT GGGAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAGG AAAATGTTTC TGATGAAAAC TCTTGGAAAT CAGAAGCTGA AATTGACATG 
GAATTGTTCC ATCAGAACCA TCTTATTGTG AAAGTTCCTC CATATCATGA CCAACATATA 
ACTTTGCCTG TATCAGTGGG AATATATGTT GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTTA CTTACACTCC AGATCCAGCA GCTGGTGCTT TGAGTGTAAA TGTGAAAAAG 
GAGATATCTA GTCCAGCAAG ACCTTGCTCT TTTGAAGAAG CCTTGAAAGC AATGAAAACG 
ACTGGCTGTA ACGTAGATAA GGTAACTATC CTTCCTAATG CCCTGATCAC TCCACTCATA 
TCGAGCAGCA TGATTAAGAC TGAAGATGTT ACTCCAATGG AAGTAACATC AGAGAAAAGA 
TCTTCCCCAA TTTTTCAGAC TACAAAGACA GTCGGATCAA CCCAGCAAAC TTTAGAAACT 
ATTTCTAACA TAGCGGGAAA TGCATCCTTT TCATCACCAT CATCTTCTCA CTTATCTCCT 
GAAAATGAAA ATCAGCAGCA GCTTCAGCCC AAGGCATACA ACCCGGAGAC TCTGACAACT 
ATCCAAACGC AAGACATCTC ACAGCCAGGG ACTTTTCCAG CGGTTTCTGC TTCTAGTCAG 
CTGCCCAGCA GCGATGCACT GCTGCAGCAG GCGGCACAGT TTCAGACAAG AGACGCTCAG 
TCCAGAGACA CAATGCAGTC AGACTCAGTG GTTAACTTGT CACAGTTGAC TGAGGCACCA 
CAGCAGCAGC AGTCCCCACT GCAAGAACAA GCACAGATAC CATCAAATAT TTTTCCGTCG 
CCAAATAGTG TGAGCCAGCT CCAGAGTACT ATTCAGCAGC TGCAAGCAGG AAGTTTTACA 
GGCAGCACTG CTAGTGGCAG CAATGGAAGT GTTGACTTGG TCCAGCAGGT TTTAGAGGCA 
CAACAACAGT TATCGTCTGT TCTGTTTTCT ACTCCAGATG GTAATGAGAA TGTTCAAGAA 
CAGCTGAATG CAGACATTTT CCAAGTCAGT CAAATTCAAA ATAGTGTAAG CCCTGGAATG 
TTTTCCTCAA CAGAGTCTGC AGTTCACACC AGACCAGATA ACTTACTACC TGGGAGGGCC 
GACAGCGTCC ATCAGCAGAC TGAAAACACA CTGTCTAGTC AGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AACAGCAGCA GCAAGTGATA GAGTCTTCAG CTGCCATGGT GATGGAGATG 
CAGCAGAGCA TTTGCCAAGC AGCAGCCCAG ATTCAGTCAG AACTGTTTCC TTCAGCTGCT 
TCAGCAAATG GAAGCCTTCA GCAGTCTCCA GTTTACCAGC AGCCTTCTCA CATGATGAGC 
GCCCTGCCTA CCAGCGAGGA CATGCAAATG CAGTGTGAAT TGTTCTCTTC CCCCCCTGCA 
GTTTCTGGAA ATGAAACATC CACCACCACC ACACCGCAGG TGGCAACCCC CGGCTCTACT 
ATGTTTCAGC CGCCAAATTC AGGCGATGGA GAAGAAACGG GAGCACAAGC AAAACAGATT 
CAGAGCAGTG TCTTTCAGAC AATGGTCCAG ATGCAGCACA GTGGGGACAG CCAACCTCAA 
GTAAACCTCT TTTCATCTAC AAAAAACATA ATGAGTGTTC AGAGTAATGG TACCCAGCAA 
CAAGGGAATA GCTTGTTCCA GCAGGGGAGC GAGATGCTGT CACTTCAGTC TGGGAGCTTC 
TTGCAGCAGT CTTCTCATTC CCAGGCTCAG CTCTTTCACC CTCAGAACCC TATTGCTGAT 
GCTCAGAGCC TTTCCCAGGA GACTCAAGGT CCAATGTTTC ATAGCGCCAA TCCCATCGTC 
CACAGTCAGA CTTCCACAGC ATCCTCCGAA CAGCTGCAGC CTTCCATGTT TCACTCGCAG 
AGTACCATTG CTGTACTACA GGGCTCTTCA GTCCCTCAGG ACCAGCAGTC ACCCAACATA 
TATCTTTCCC AAAGTTCCAT CAGTAATCTT CAAACTAACA CAGTAGCCCA AGAAGAGCAG 
ATTTCATTTT TTTCAGCACA GAACTCAATT TCTCCACTTC AGTCAACATC AAACACTGAG 
CAGCAAGCTG CTTTTCAACA GCAGCCTCCA ATCTCGCACA TCCAGACCCC TCTTCTTTCC 
CAGGAACAGG CACAGCCCTC TCAGCAAGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC 
CTTCCTGCTA ATCCAATGCC TCAGAACCAG CAAGGCCCAA TTTTCCAGAC ACAGCGCCCA 
ATAGTTGGCA TGCAGAGTAA CTCTCCATCC CAGGAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AGCAGCAGCA GCAGCAGCAG AGCATTTTAT TTAGTAATCA GAATGCCATG 
GCTACAATGG CCTCCCAGAA GCAGCCACCA CCAAACATGA TATTTAGCCC AAACCAGAAC 
CCGATGGCTA GTCAAGAGCA GCAGAACCAG TCAATTTTTC ACCAGCAAAG TAATATGGCC 
CCAATGAACC AAGAGCAGCA GCCCATGCAG TTTCAGAATC AGCCTACGGT TTCCTCACTT 
CAGAACCCAG GTCCTACGCC ATCTGAGTCA CCACAGACCT CCTTGTTCCA TAGTTCTCCT 
CAGATCCAGT TGGTTCAAGG GTCACCTAGT TCTCAAGAGC AACAAGTAAC ACTCTTCCTC 
TCTCCAGCAT CCATGTCTGC ATTGCAAACC AGTATAAACC AACCAGACAT GCAGCAGTCT 
CCTCTTTATT CCCCACAGAA CAACATCCCT GGAATCCAAG GAAGCACTTC TTCACCCCAG 
CCACAGGCTG CTTTATTTCA CAACACTACA GGAGGCACAA TAAACCAACT ACAAAATTCT 
CCTGGCTCAT CTCAGCAGAC TTCAGGAATG TTCTTATTTG GCATTCAAAA TAATTGTAGT 
CAGCTGTTAA CTTCTGGGCC AGCTACATTG CCAGAGCAGC TGATGGCCAT AAATCAGCCG 
GGCCAACCAC AAAATGAGGG CCAATCTTCT GTGACAACAC TTCTTTCTCA GCAAATGCCA 
GAATCTGCCC CACTGGCCTC CTCGGTGAAC AACAGTCAGA ACATGGAAAA GATCGATTTG 
CTTGTTTCGT TGCAAAGCCA AGGGAACAAT TTAACCGGCT CCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_006255555.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255617.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X6, mRNA.

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CloneID	ORa38899
Clone ID Related Accession (Same CDS sequence)	XM_006255555.3 , XM_008772505.2 , XM_006255556.3
Accession Version	XM_006255555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255617.1
CDS	344..4318
Translation	MLLQLPPPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_006255555.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255555.3

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_008772505.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770727.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X5, mRNA.

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CloneID	ORa38899
Clone ID Related Accession (Same CDS sequence)	XM_006255555.3 , XM_008772505.2 , XM_006255556.3
Accession Version	XM_008772505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770727.1
CDS	796..4770
Translation	MLLQLPPPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_008772505.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772505.2

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_006255556.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255618.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa38899
Clone ID Related Accession (Same CDS sequence)	XM_006255555.3 , XM_008772505.2 , XM_006255556.3
Accession Version	XM_006255556.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255618.1
CDS	282..4256
Translation	MLLQLPPPPWAVLAAPLPPLPALPFILPQSPTARLCKWRAAPQPWGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_006255556.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255556.3

ORF Insert Sequence:

ATGCTTCTTC AGCTCCCTCC TCCTCCATGG GCGGTGCTTG CAGCTCCTTT ACCACCTCTT 
CCAGCCCTAC CATTTATTCT ACCTCAGTCA CCGACAGCAA GGCTATGCAA GTGGAGAGCT 
GCTCCTCAGC CGTGGGGAAC ATTGGACAGC CAAAAGGGAA CTGGAGTAAA GAAGAGTCCT 
ATGTTGTGTG GACAGTATCC GGTTAAAAGT GAGGGGAAGG AGCTGAAGAT AGTGGTACAG 
CCTGAAACCC AACACCGAGC CCGGTACCTG ACAGAGGGCA GCCGAGGCTC CGTAAAAGAT 
AGAACACAGC AAGGCTTTCC TACGGTGAAG CTGGAAGGTC ATAATGAGCC AGTGGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTAAAGCCAC ACGGATTCTA TCAGGCCTGC 
AGAGTGACCG GGCGAAACAC AACTCCGTGC AAAGAAGTGG ACATCGAAGG CACCACTGTG 
ATAGAGGTTG GCCTCGACCC TAGCAGCAAC ATGACGCTGG CAGTGGACTG TGTAGGAATA 
CTGAAGCTGA GAAATGCTGA TGTTGAGGCC AGAATTGGAA TTGCTGGTTC TAAGAAAAAG 
AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACGA GGAAAGATGG CTCTACTCTG 
ACATTGCAGA CACCTTCTTC CCCAATTTTA TGCACTCAGC CAGCAGGAGT TCCTGAGATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTGTT TTTAATTGGG 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAGGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAATCAG AAGCTGAAAT TGACATGGAA TTGTTCCATC AGAACCATCT TATTGTGAAA 
GTTCCTCCAT ATCATGACCA ACATATAACT TTGCCTGTAT CAGTGGGAAT ATATGTTGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTTACTT ACACTCCAGA TCCAGCAGCT 
GGTGCTTTGA GTGTAAATGT GAAAAAGGAG ATATCTAGTC CAGCAAGACC TTGCTCTTTT 
GAAGAAGCCT TGAAAGCAAT GAAAACGACT GGCTGTAACG TAGATAAGGT AACTATCCTT 
CCTAATGCCC TGATCACTCC ACTCATATCG AGCAGCATGA TTAAGACTGA AGATGTTACT 
CCAATGGAAG TAACATCAGA GAAAAGATCT TCCCCAATTT TTCAGACTAC AAAGACAGTC 
GGATCAACCC AGCAAACTTT AGAAACTATT TCTAACATAG CGGGAAATGC ATCCTTTTCA 
TCACCATCAT CTTCTCACTT ATCTCCTGAA AATGAAAATC AGCAGCAGCT TCAGCCCAAG 
GCATACAACC CGGAGACTCT GACAACTATC CAAACGCAAG ACATCTCACA GCCAGGGACT 
TTTCCAGCGG TTTCTGCTTC TAGTCAGCTG CCCAGCAGCG ATGCACTGCT GCAGCAGGCG 
GCACAGTTTC AGACAAGAGA CGCTCAGTCC AGAGACACAA TGCAGTCAGA CTCAGTGGTT 
AACTTGTCAC AGTTGACTGA GGCACCACAG CAGCAGCAGT CCCCACTGCA AGAACAAGCA 
CAGATACCAT CAAATATTTT TCCGTCGCCA AATAGTGTGA GCCAGCTCCA GAGTACTATT 
CAGCAGCTGC AAGCAGGAAG TTTTACAGGC AGCACTGCTA GTGGCAGCAA TGGAAGTGTT 
GACTTGGTCC AGCAGGTTTT AGAGGCACAA CAACAGTTAT CGTCTGTTCT GTTTTCTACT 
CCAGATGGTA ATGAGAATGT TCAAGAACAG CTGAATGCAG ACATTTTCCA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGTCTGCAGT TCACACCAGA 
CCAGATAACT TACTACCTGG GAGGGCCGAC AGCGTCCATC AGCAGACTGA AAACACACTG 
TCTAGTCAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA AGTGATAGAG 
TCTTCAGCTG CCATGGTGAT GGAGATGCAG CAGAGCATTT GCCAAGCAGC AGCCCAGATT 
CAGTCAGAAC TGTTTCCTTC AGCTGCTTCA GCAAATGGAA GCCTTCAGCA GTCTCCAGTT 
TACCAGCAGC CTTCTCACAT GATGAGCGCC CTGCCTACCA GCGAGGACAT GCAAATGCAG 
TGTGAATTGT TCTCTTCCCC CCCTGCAGTT TCTGGAAATG AAACATCCAC CACCACCACA 
CCGCAGGTGG CAACCCCCGG CTCTACTATG TTTCAGCCGC CAAATTCAGG CGATGGAGAA 
GAAACGGGAG CACAAGCAAA ACAGATTCAG AGCAGTGTCT TTCAGACAAT GGTCCAGATG 
CAGCACAGTG GGGACAGCCA ACCTCAAGTA AACCTCTTTT CATCTACAAA AAACATAATG 
AGTGTTCAGA GTAATGGTAC CCAGCAACAA GGGAATAGCT TGTTCCAGCA GGGGAGCGAG 
ATGCTGTCAC TTCAGTCTGG GAGCTTCTTG CAGCAGTCTT CTCATTCCCA GGCTCAGCTC 
TTTCACCCTC AGAACCCTAT TGCTGATGCT CAGAGCCTTT CCCAGGAGAC TCAAGGTCCA 
ATGTTTCATA GCGCCAATCC CATCGTCCAC AGTCAGACTT CCACAGCATC CTCCGAACAG 
CTGCAGCCTT CCATGTTTCA CTCGCAGAGT ACCATTGCTG TACTACAGGG CTCTTCAGTC 
CCTCAGGACC AGCAGTCACC CAACATATAT CTTTCCCAAA GTTCCATCAG TAATCTTCAA 
ACTAACACAG TAGCCCAAGA AGAGCAGATT TCATTTTTTT CAGCACAGAA CTCAATTTCT 
CCACTTCAGT CAACATCAAA CACTGAGCAG CAAGCTGCTT TTCAACAGCA GCCTCCAATC 
TCGCACATCC AGACCCCTCT TCTTTCCCAG GAACAGGCAC AGCCCTCTCA GCAAGGTTTA 
TTTCAGCCTC AGGTGGCCCT GGGCTCCCTT CCTGCTAATC CAATGCCTCA GAACCAGCAA 
GGCCCAATTT TCCAGACACA GCGCCCAATA GTTGGCATGC AGAGTAACTC TCCATCCCAG 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAGC AGCAGCAGCA GCAGCAGAGC 
ATTTTATTTA GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA 
AACATGATAT TTAGCCCAAA CCAGAACCCG ATGGCTAGTC AAGAGCAGCA GAACCAGTCA 
ATTTTTCACC AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAGTTT 
CAGAATCAGC CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCCATC TGAGTCACCA 
CAGACCTCCT TGTTCCATAG TTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT 
CAAGAGCAAC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT 
ATAAACCAAC CAGACATGCA GCAGTCTCCT CTTTATTCCC CACAGAACAA CATCCCTGGA 
ATCCAAGGAA GCACTTCTTC ACCCCAGCCA CAGGCTGCTT TATTTCACAA CACTACAGGA 
GGCACAATAA ACCAACTACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC 
TTATTTGGCA TTCAAAATAA TTGTAGTCAG CTGTTAACTT CTGGGCCAGC TACATTGCCA 
GAGCAGCTGA TGGCCATAAA TCAGCCGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG 
ACAACACTTC TTTCTCAGCA AATGCCAGAA TCTGCCCCAC TGGCCTCCTC GGTGAACAAC 
AGTCAGAACA TGGAAAAGAT CGATTTGCTT GTTTCGTTGC AAAGCCAAGG GAACAATTTA 
ACCGGCTCCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_008772503.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770725.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X1, mRNA.

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CloneID	ORa46552
Clone ID Related Accession (Same CDS sequence)	XM_008772503.2
Accession Version	XM_008772503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ACCTAGAGAA 
ACATCTGCAG CATCAATGAG TCAGACAAGC GGTGGCGAGG CAGGCTCGCC TCCTCCAGCT 
GTAGTTGCTG CCGATGCTTC TTCAGCTCCC TCCTCCTCCA TGGGCGGTGC TTGCAGCTCC 
TTTACCACCT CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG 
CAAGTGGAGA GCTGCTCCTC AGCCGTGGGG GTAAGTAACA GAGGGGTAAG TGAAAAGCAG 
TTAACCGGTA ACACAGTTCA GCAGCATCCA TCAACCCCGA AGAGGCACAC AGTTTTGTAC 
ATCTCACCAC CACCTGAGGA CTTGCTGGAT AACAGTCGGA TGTCCTGCCA GGATGAGGGG 
TGTGGATTGG AATCTGAGCA GAGCTGCAGT ATGTGGATGG AGGATTCCCC CTCCAACTTC 
AGTAACATGA GCACCAGTTC CTACAATGAT AACACTGAGG TACCTCGTAA ATCACGAAAA 
CGAAATCCAA AGCAGAGGCC GGGGGTCAAA CGACGAGATT GTGAAGAATC TAATATGGAT 
ATATTTGATG CCGACAGTGC CAAAGCACCT CACTATGTGC TTTCTCAGCT TACCACGGAC 
AACAAAGGCA ACTCAAAAGC TGGAAATGGA ACATTGGACA GCCAAAAGGG AACTGGAGTA 
AAGAAGAGTC CTATGTTGTG TGGACAGTAT CCGGTTAAAA GTGAGGGGAA GGAGCTGAAG 
ATAGTGGTAC AGCCTGAAAC CCAACACCGA GCCCGGTACC TGACAGAGGG CAGCCGAGGC 
TCCGTAAAAG ATAGAACACA GCAAGGCTTT CCTACGGTGA AGCTGGAAGG TCATAATGAG 
CCAGTGGTGT TGCAGGTGTT TGTGGGCAAT GACTCTGGTC GAGTAAAGCC ACACGGATTC 
TATCAGGCCT GCAGAGTGAC CGGGCGAAAC ACAACTCCGT GCAAAGAAGT GGACATCGAA 
GGCACCACTG TGATAGAGGT TGGCCTCGAC CCTAGCAGCA ACATGACGCT GGCAGTGGAC 
TGTGTAGGAA TACTGAAGCT GAGAAATGCT GATGTTGAGG CCAGAATTGG AATTGCTGGT 
TCTAAGAAAA AGAGCACTCG TGCCAGATTG GTTTTTCGAG TTAATATCAC GAGGAAAGAT 
GGCTCTACTC TGACATTGCA GACACCTTCT TCCCCAATTT TATGCACTCA GCCAGCAGGA 
GTTCCTGAGA TCTTAAAGAA AAGCTTGCAT AGCTGTTCAG TGAAAGGAGA GGAAGAAGTG 
TTTTTAATTG GGAAGAACTT TCTGAAAGGA ACTAAAGTTA TTTTCCAGGA AAATGTTTCT 
GATGAAAACT CTTGGAAATC AGAAGCTGAA ATTGACATGG AATTGTTCCA TCAGAACCAT 
CTTATTGTGA AAGTTCCTCC ATATCATGAC CAACATATAA CTTTGCCTGT ATCAGTGGGA 
ATATATGTTG TGACCAATGC TGGAAGATCT CATGATGTTC AGCCATTTAC TTACACTCCA 
GATCCAGCTG GTGCTTTGAG TGTAAATGTG AAAAAGGAGA TATCTAGTCC AGCAAGACCT 
TGCTCTTTTG AAGAAGCCTT GAAAGCAATG AAAACGACTG GCTGTAACGT AGATAAGGTA 
ACTATCCTTC CTAATGCCCT GATCACTCCA CTCATATCGA GCAGCATGAT TAAGACTGAA 
GATGTTACTC CAATGGAAGT AACATCAGAG AAAAGATCTT CCCCAATTTT TCAGACTACA 
AAGACAGTCG GATCAACCCA GCAAACTTTA GAAACTATTT CTAACATAGC GGGAAATGCA 
TCCTTTTCAT CACCATCATC TTCTCACTTA TCTCCTGAAA ATGAAAATCA GCAGCAGCTT 
CAGCCCAAGG CATACAACCC GGAGACTCTG ACAACTATCC AAACGCAAGA CATCTCACAG 
CCAGGGACTT TTCCAGCGGT TTCTGCTTCT AGTCAGCTGC CCAGCAGCGA TGCACTGCTG 
CAGCAGGCGG CACAGTTTCA GACAAGAGAC GCTCAGTCCA GAGACACAAT GCAGTCAGAC 
TCAGTGGTTA ACTTGTCACA GTTGACTGAG GCACCACAGC AGCAGCAGTC CCCACTGCAA 
GAACAAGCAC AGATACCATC AAATATTTTT CCGTCGCCAA ATAGTGTGAG CCAGCTCCAG 
AGTACTATTC AGCAGCTGCA AGCAGGAAGT TTTACAGGCA GCACTGCTAG TGGCAGCAAT 
GGAAGTGTTG ACTTGGTCCA GCAGGTTTTA GAGGCACAAC AACAGTTATC GTCTGTTCTG 
TTTTCTACTC CAGATGGTAA TGAGAATGTT CAAGAACAGC TGAATGCAGA CATTTTCCAA 
GTCAGTCAAA TTCAAAATAG TGTAAGCCCT GGAATGTTTT CCTCAACAGA GTCTGCAGTT 
CACACCAGAC CAGATAACTT ACTACCTGGG AGGGCCGACA GCGTCCATCA GCAGACTGAA 
AACACACTGT CTAGTCAGCA GCAGCAACAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAA 
GTGATAGAGT CTTCAGCTGC CATGGTGATG GAGATGCAGC AGAGCATTTG CCAAGCAGCA 
GCCCAGATTC AGTCAGAACT GTTTCCTTCA GCTGCTTCAG CAAATGGAAG CCTTCAGCAG 
TCTCCAGTTT ACCAGCAGCC TTCTCACATG ATGAGCGCCC TGCCTACCAG CGAGGACATG 
CAAATGCAGT GTGAATTGTT CTCTTCCCCC CCTGCAGTTT CTGGAAATGA AACATCCACC 
ACCACCACAC CGCAGGTGGC AACCCCCGGC TCTACTATGT TTCAGCCGCC AAATTCAGGC 
GATGGAGAAG AAACGGGAGC ACAAGCAAAA CAGATTCAGA GCAGTGTCTT TCAGACAATG 
GTCCAGATGC AGCACAGTGG GGACAGCCAA CCTCAAGTAA ACCTCTTTTC ATCTACAAAA 
AACATAATGA GTGTTCAGAG TAATGGTACC CAGCAACAAG GGAATAGCTT GTTCCAGCAG 
GGGAGCGAGA TGCTGTCACT TCAGTCTGGG AGCTTCTTGC AGCAGTCTTC TCATTCCCAG 
GCTCAGCTCT TTCACCCTCA GAACCCTATT GCTGATGCTC AGAGCCTTTC CCAGGAGACT 
CAAGGTCCAA TGTTTCATAG CGCCAATCCC ATCGTCCACA GTCAGACTTC CACAGCATCC 
TCCGAACAGC TGCAGCCTTC CATGTTTCAC TCGCAGAGTA CCATTGCTGT ACTACAGGGC 
TCTTCAGTCC CTCAGGACCA GCAGTCACCC AACATATATC TTTCCCAAAG TTCCATCAGT 
AATCTTCAAA CTAACACAGT AGCCCAAGAA GAGCAGATTT CATTTTTTTC AGCACAGAAC 
TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT TCAACAGCAG 
CCTCCAATCT CGCACATCCA GACCCCTCTT CTTTCCCAGG AACAGGCACA GCCCTCTCAG 
CAAGGTTTAT TTCAGCCTCA GGTGGCCCTG GGCTCCCTTC CTGCTAATCC AATGCCTCAG 
AACCAGCAAG GCCCAATTTT CCAGACACAG CGCCCAATAG TTGGCATGCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AACAACAGCA GCAGCAGCAG 
CAGCAGAGCA TTTTATTTAG TAATCAGAAT GCCATGGCTA CAATGGCCTC CCAGAAGCAG 
CCACCACCAA ACATGATATT TAGCCCAAAC CAGAACCCGA TGGCTAGTCA AGAGCAGCAG 
AACCAGTCAA TTTTTCACCA GCAAAGTAAT ATGGCCCCAA TGAACCAAGA GCAGCAGCCC 
ATGCAGTTTC AGAATCAGCC TACGGTTTCC TCACTTCAGA ACCCAGGTCC TACGCCATCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TCCAGTTGGT TCAAGGGTCA 
CCTAGTTCTC AAGAGCAACA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG 
CAAACCAGTA TAAACCAACC AGACATGCAG CAGTCTCCTC TTTATTCCCC ACAGAACAAC 
ATCCCTGGAA TCCAAGGAAG CACTTCTTCA CCCCAGCCAC AGGCTGCTTT ATTTCACAAC 
ACTACAGGAG GCACAATAAA CCAACTACAA AATTCTCCTG GCTCATCTCA GCAGACTTCA 
GGAATGTTCT TATTTGGCAT TCAAAATAAT TGTAGTCAGC TGTTAACTTC TGGGCCAGCT 
ACATTGCCAG AGCAGCTGAT GGCCATAAAT CAGCCGGGCC AACCACAAAA TGAGGGCCAA 
TCTTCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAAT CTGCCCCACT GGCCTCCTCG 
GTGAACAACA GTCAGAACAT GGAAAAGATC GATTTGCTTG TTTCGTTGCA AAGCCAAGGG 
AACAATTTAA CCGGCTCCTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770725.1
CDS	792..5435
Translation	MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPPRETSAASMSQTSGGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_008772503.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772503.2

ORF Insert Sequence:

ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAATC GCCCAAGTCC 
CTGTACTCGC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA 
TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ACCTAGAGAA 
ACATCTGCAG CATCAATGAG TCAGACAAGC GGTGGCGAGG CAGGCTCGCC TCCTCCAGCT 
GTAGTTGCTG CCGATGCTTC TTCAGCTCCC TCCTCCTCCA TGGGCGGTGC TTGCAGCTCC 
TTTACCACCT CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG 
CAAGTGGAGA GCTGCTCCTC AGCCGTGGGG GTAAGTAACA GAGGGGTAAG TGAAAAGCAG 
TTAACCGGTA ACACAGTTCA GCAGCATCCA TCAACCCCGA AGAGGCACAC AGTTTTGTAC 
ATCTCACCAC CACCTGAGGA CTTGCTGGAT AACAGTCGGA TGTCCTGCCA GGATGAGGGG 
TGTGGATTGG AATCTGAGCA GAGCTGCAGT ATGTGGATGG AGGATTCCCC CTCCAACTTC 
AGTAACATGA GCACCAGTTC CTACAATGAT AACACTGAGG TACCTCGTAA ATCACGAAAA 
CGAAATCCAA AGCAGAGGCC GGGGGTCAAA CGACGAGATT GTGAAGAATC TAATATGGAT 
ATATTTGATG CCGACAGTGC CAAAGCACCT CACTATGTGC TTTCTCAGCT TACCACGGAC 
AACAAAGGCA ACTCAAAAGC TGGAAATGGA ACATTGGACA GCCAAAAGGG AACTGGAGTA 
AAGAAGAGTC CTATGTTGTG TGGACAGTAT CCGGTTAAAA GTGAGGGGAA GGAGCTGAAG 
ATAGTGGTAC AGCCTGAAAC CCAACACCGA GCCCGGTACC TGACAGAGGG CAGCCGAGGC 
TCCGTAAAAG ATAGAACACA GCAAGGCTTT CCTACGGTGA AGCTGGAAGG TCATAATGAG 
CCAGTGGTGT TGCAGGTGTT TGTGGGCAAT GACTCTGGTC GAGTAAAGCC ACACGGATTC 
TATCAGGCCT GCAGAGTGAC CGGGCGAAAC ACAACTCCGT GCAAAGAAGT GGACATCGAA 
GGCACCACTG TGATAGAGGT TGGCCTCGAC CCTAGCAGCA ACATGACGCT GGCAGTGGAC 
TGTGTAGGAA TACTGAAGCT GAGAAATGCT GATGTTGAGG CCAGAATTGG AATTGCTGGT 
TCTAAGAAAA AGAGCACTCG TGCCAGATTG GTTTTTCGAG TTAATATCAC GAGGAAAGAT 
GGCTCTACTC TGACATTGCA GACACCTTCT TCCCCAATTT TATGCACTCA GCCAGCAGGA 
GTTCCTGAGA TCTTAAAGAA AAGCTTGCAT AGCTGTTCAG TGAAAGGAGA GGAAGAAGTG 
TTTTTAATTG GGAAGAACTT TCTGAAAGGA ACTAAAGTTA TTTTCCAGGA AAATGTTTCT 
GATGAAAACT CTTGGAAATC AGAAGCTGAA ATTGACATGG AATTGTTCCA TCAGAACCAT 
CTTATTGTGA AAGTTCCTCC ATATCATGAC CAACATATAA CTTTGCCTGT ATCAGTGGGA 
ATATATGTTG TGACCAATGC TGGAAGATCT CATGATGTTC AGCCATTTAC TTACACTCCA 
GATCCAGCTG GTGCTTTGAG TGTAAATGTG AAAAAGGAGA TATCTAGTCC AGCAAGACCT 
TGCTCTTTTG AAGAAGCCTT GAAAGCAATG AAAACGACTG GCTGTAACGT AGATAAGGTA 
ACTATCCTTC CTAATGCCCT GATCACTCCA CTCATATCGA GCAGCATGAT TAAGACTGAA 
GATGTTACTC CAATGGAAGT AACATCAGAG AAAAGATCTT CCCCAATTTT TCAGACTACA 
AAGACAGTCG GATCAACCCA GCAAACTTTA GAAACTATTT CTAACATAGC GGGAAATGCA 
TCCTTTTCAT CACCATCATC TTCTCACTTA TCTCCTGAAA ATGAAAATCA GCAGCAGCTT 
CAGCCCAAGG CATACAACCC GGAGACTCTG ACAACTATCC AAACGCAAGA CATCTCACAG 
CCAGGGACTT TTCCAGCGGT TTCTGCTTCT AGTCAGCTGC CCAGCAGCGA TGCACTGCTG 
CAGCAGGCGG CACAGTTTCA GACAAGAGAC GCTCAGTCCA GAGACACAAT GCAGTCAGAC 
TCAGTGGTTA ACTTGTCACA GTTGACTGAG GCACCACAGC AGCAGCAGTC CCCACTGCAA 
GAACAAGCAC AGATACCATC AAATATTTTT CCGTCGCCAA ATAGTGTGAG CCAGCTCCAG 
AGTACTATTC AGCAGCTGCA AGCAGGAAGT TTTACAGGCA GCACTGCTAG TGGCAGCAAT 
GGAAGTGTTG ACTTGGTCCA GCAGGTTTTA GAGGCACAAC AACAGTTATC GTCTGTTCTG 
TTTTCTACTC CAGATGGTAA TGAGAATGTT CAAGAACAGC TGAATGCAGA CATTTTCCAA 
GTCAGTCAAA TTCAAAATAG TGTAAGCCCT GGAATGTTTT CCTCAACAGA GTCTGCAGTT 
CACACCAGAC CAGATAACTT ACTACCTGGG AGGGCCGACA GCGTCCATCA GCAGACTGAA 
AACACACTGT CTAGTCAGCA GCAGCAACAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAA 
GTGATAGAGT CTTCAGCTGC CATGGTGATG GAGATGCAGC AGAGCATTTG CCAAGCAGCA 
GCCCAGATTC AGTCAGAACT GTTTCCTTCA GCTGCTTCAG CAAATGGAAG CCTTCAGCAG 
TCTCCAGTTT ACCAGCAGCC TTCTCACATG ATGAGCGCCC TGCCTACCAG CGAGGACATG 
CAAATGCAGT GTGAATTGTT CTCTTCCCCC CCTGCAGTTT CTGGAAATGA AACATCCACC 
ACCACCACAC CGCAGGTGGC AACCCCCGGC TCTACTATGT TTCAGCCGCC AAATTCAGGC 
GATGGAGAAG AAACGGGAGC ACAAGCAAAA CAGATTCAGA GCAGTGTCTT TCAGACAATG 
GTCCAGATGC AGCACAGTGG GGACAGCCAA CCTCAAGTAA ACCTCTTTTC ATCTACAAAA 
AACATAATGA GTGTTCAGAG TAATGGTACC CAGCAACAAG GGAATAGCTT GTTCCAGCAG 
GGGAGCGAGA TGCTGTCACT TCAGTCTGGG AGCTTCTTGC AGCAGTCTTC TCATTCCCAG 
GCTCAGCTCT TTCACCCTCA GAACCCTATT GCTGATGCTC AGAGCCTTTC CCAGGAGACT 
CAAGGTCCAA TGTTTCATAG CGCCAATCCC ATCGTCCACA GTCAGACTTC CACAGCATCC 
TCCGAACAGC TGCAGCCTTC CATGTTTCAC TCGCAGAGTA CCATTGCTGT ACTACAGGGC 
TCTTCAGTCC CTCAGGACCA GCAGTCACCC AACATATATC TTTCCCAAAG TTCCATCAGT 
AATCTTCAAA CTAACACAGT AGCCCAAGAA GAGCAGATTT CATTTTTTTC AGCACAGAAC 
TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT TCAACAGCAG 
CCTCCAATCT CGCACATCCA GACCCCTCTT CTTTCCCAGG AACAGGCACA GCCCTCTCAG 
CAAGGTTTAT TTCAGCCTCA GGTGGCCCTG GGCTCCCTTC CTGCTAATCC AATGCCTCAG 
AACCAGCAAG GCCCAATTTT CCAGACACAG CGCCCAATAG TTGGCATGCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AACAACAGCA GCAGCAGCAG 
CAGCAGAGCA TTTTATTTAG TAATCAGAAT GCCATGGCTA CAATGGCCTC CCAGAAGCAG 
CCACCACCAA ACATGATATT TAGCCCAAAC CAGAACCCGA TGGCTAGTCA AGAGCAGCAG 
AACCAGTCAA TTTTTCACCA GCAAAGTAAT ATGGCCCCAA TGAACCAAGA GCAGCAGCCC 
ATGCAGTTTC AGAATCAGCC TACGGTTTCC TCACTTCAGA ACCCAGGTCC TACGCCATCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TCCAGTTGGT TCAAGGGTCA 
CCTAGTTCTC AAGAGCAACA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG 
CAAACCAGTA TAAACCAACC AGACATGCAG CAGTCTCCTC TTTATTCCCC ACAGAACAAC 
ATCCCTGGAA TCCAAGGAAG CACTTCTTCA CCCCAGCCAC AGGCTGCTTT ATTTCACAAC 
ACTACAGGAG GCACAATAAA CCAACTACAA AATTCTCCTG GCTCATCTCA GCAGACTTCA 
GGAATGTTCT TATTTGGCAT TCAAAATAAT TGTAGTCAGC TGTTAACTTC TGGGCCAGCT 
ACATTGCCAG AGCAGCTGAT GGCCATAAAT CAGCCGGGCC AACCACAAAA TGAGGGCCAA 
TCTTCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAAT CTGCCCCACT GGCCTCCTCG 
GTGAACAACA GTCAGAACAT GGAAAAGATC GATTTGCTTG TTTCGTTGCA AAGCCAAGGG 
AACAATTTAA CCGGCTCCTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfat5 ( XM_008772504.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770726.1 Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X3, mRNA.

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CloneID	ORa46553
Clone ID Related Accession (Same CDS sequence)	XM_008772504.2
Accession Version	XM_008772504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTG TATTTACTCT TGGATTGTAC ACCGCAGATG CTTCTTCAGC TCCCTCCTCC 
TCCATGGGCG GTGCTTGCAG CTCCTTTACC ACCTCTTCCA GCCCTACCAT TTATTCTACC 
TCAGTCACCG ACAGCAAGGC TATGCAAGTG GAGAGCTGCT CCTCAGCCGT GGGGGTAAGT 
AACAGAGGGG TAAGTGAAAA GCAGTTAACC GGTAACACAG TTCAGCAGCA TCCATCAACC 
CCGAAGAGGC ACACAGTTTT GTACATCTCA CCACCACCTG AGGACTTGCT GGATAACAGT 
CGGATGTCCT GCCAGGATGA GGGGTGTGGA TTGGAATCTG AGCAGAGCTG CAGTATGTGG 
ATGGAGGATT CCCCCTCCAA CTTCAGTAAC ATGAGCACCA GTTCCTACAA TGATAACACT 
GAGGTACCTC GTAAATCACG AAAACGAAAT CCAAAGCAGA GGCCGGGGGT CAAACGACGA 
GATTGTGAAG AATCTAATAT GGATATATTT GATGCCGACA GTGCCAAAGC ACCTCACTAT 
GTGCTTTCTC AGCTTACCAC GGACAACAAA GGCAACTCAA AAGCTGGAAA TGGAACATTG 
GACAGCCAAA AGGGAACTGG AGTAAAGAAG AGTCCTATGT TGTGTGGACA GTATCCGGTT 
AAAAGTGAGG GGAAGGAGCT GAAGATAGTG GTACAGCCTG AAACCCAACA CCGAGCCCGG 
TACCTGACAG AGGGCAGCCG AGGCTCCGTA AAAGATAGAA CACAGCAAGG CTTTCCTACG 
GTGAAGCTGG AAGGTCATAA TGAGCCAGTG GTGTTGCAGG TGTTTGTGGG CAATGACTCT 
GGTCGAGTAA AGCCACACGG ATTCTATCAG GCCTGCAGAG TGACCGGGCG AAACACAACT 
CCGTGCAAAG AAGTGGACAT CGAAGGCACC ACTGTGATAG AGGTTGGCCT CGACCCTAGC 
AGCAACATGA CGCTGGCAGT GGACTGTGTA GGAATACTGA AGCTGAGAAA TGCTGATGTT 
GAGGCCAGAA TTGGAATTGC TGGTTCTAAG AAAAAGAGCA CTCGTGCCAG ATTGGTTTTT 
CGAGTTAATA TCACGAGGAA AGATGGCTCT ACTCTGACAT TGCAGACACC TTCTTCCCCA 
ATTTTATGCA CTCAGCCAGC AGGAGTTCCT GAGATCTTAA AGAAAAGCTT GCATAGCTGT 
TCAGTGAAAG GAGAGGAAGA AGTGTTTTTA ATTGGGAAGA ACTTTCTGAA AGGAACTAAA 
GTTATTTTCC AGGAAAATGT TTCTGATGAA AACTCTTGGA AATCAGAAGC TGAAATTGAC 
ATGGAATTGT TCCATCAGAA CCATCTTATT GTGAAAGTTC CTCCATATCA TGACCAACAT 
ATAACTTTGC CTGTATCAGT GGGAATATAT GTTGTGACCA ATGCTGGAAG ATCTCATGAT 
GTTCAGCCAT TTACTTACAC TCCAGATCCA GCAGCTGGTG CTTTGAGTGT AAATGTGAAA 
AAGGAGATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AAGCCTTGAA AGCAATGAAA 
ACGACTGGCT GTAACGTAGA TAAGGTAACT ATCCTTCCTA ATGCCCTGAT CACTCCACTC 
ATATCGAGCA GCATGATTAA GACTGAAGAT GTTACTCCAA TGGAAGTAAC ATCAGAGAAA 
AGATCTTCCC CAATTTTTCA GACTACAAAG ACAGTCGGAT CAACCCAGCA AACTTTAGAA 
ACTATTTCTA ACATAGCGGG AAATGCATCC TTTTCATCAC CATCATCTTC TCACTTATCT 
CCTGAAAATG AAAATCAGCA GCAGCTTCAG CCCAAGGCAT ACAACCCGGA GACTCTGACA 
ACTATCCAAA CGCAAGACAT CTCACAGCCA GGGACTTTTC CAGCGGTTTC TGCTTCTAGT 
CAGCTGCCCA GCAGCGATGC ACTGCTGCAG CAGGCGGCAC AGTTTCAGAC AAGAGACGCT 
CAGTCCAGAG ACACAATGCA GTCAGACTCA GTGGTTAACT TGTCACAGTT GACTGAGGCA 
CCACAGCAGC AGCAGTCCCC ACTGCAAGAA CAAGCACAGA TACCATCAAA TATTTTTCCG 
TCGCCAAATA GTGTGAGCCA GCTCCAGAGT ACTATTCAGC AGCTGCAAGC AGGAAGTTTT 
ACAGGCAGCA CTGCTAGTGG CAGCAATGGA AGTGTTGACT TGGTCCAGCA GGTTTTAGAG 
GCACAACAAC AGTTATCGTC TGTTCTGTTT TCTACTCCAG ATGGTAATGA GAATGTTCAA 
GAACAGCTGA ATGCAGACAT TTTCCAAGTC AGTCAAATTC AAAATAGTGT AAGCCCTGGA 
ATGTTTTCCT CAACAGAGTC TGCAGTTCAC ACCAGACCAG ATAACTTACT ACCTGGGAGG 
GCCGACAGCG TCCATCAGCA GACTGAAAAC ACACTGTCTA GTCAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAAGTG ATAGAGTCTT CAGCTGCCAT GGTGATGGAG 
ATGCAGCAGA GCATTTGCCA AGCAGCAGCC CAGATTCAGT CAGAACTGTT TCCTTCAGCT 
GCTTCAGCAA ATGGAAGCCT TCAGCAGTCT CCAGTTTACC AGCAGCCTTC TCACATGATG 
AGCGCCCTGC CTACCAGCGA GGACATGCAA ATGCAGTGTG AATTGTTCTC TTCCCCCCCT 
GCAGTTTCTG GAAATGAAAC ATCCACCACC ACCACACCGC AGGTGGCAAC CCCCGGCTCT 
ACTATGTTTC AGCCGCCAAA TTCAGGCGAT GGAGAAGAAA CGGGAGCACA AGCAAAACAG 
ATTCAGAGCA GTGTCTTTCA GACAATGGTC CAGATGCAGC ACAGTGGGGA CAGCCAACCT 
CAAGTAAACC TCTTTTCATC TACAAAAAAC ATAATGAGTG TTCAGAGTAA TGGTACCCAG 
CAACAAGGGA ATAGCTTGTT CCAGCAGGGG AGCGAGATGC TGTCACTTCA GTCTGGGAGC 
TTCTTGCAGC AGTCTTCTCA TTCCCAGGCT CAGCTCTTTC ACCCTCAGAA CCCTATTGCT 
GATGCTCAGA GCCTTTCCCA GGAGACTCAA GGTCCAATGT TTCATAGCGC CAATCCCATC 
GTCCACAGTC AGACTTCCAC AGCATCCTCC GAACAGCTGC AGCCTTCCAT GTTTCACTCG 
CAGAGTACCA TTGCTGTACT ACAGGGCTCT TCAGTCCCTC AGGACCAGCA GTCACCCAAC 
ATATATCTTT CCCAAAGTTC CATCAGTAAT CTTCAAACTA ACACAGTAGC CCAAGAAGAG 
CAGATTTCAT TTTTTTCAGC ACAGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACACT 
GAGCAGCAAG CTGCTTTTCA ACAGCAGCCT CCAATCTCGC ACATCCAGAC CCCTCTTCTT 
TCCCAGGAAC AGGCACAGCC CTCTCAGCAA GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCTG CTAATCCAAT GCCTCAGAAC CAGCAAGGCC CAATTTTCCA GACACAGCGC 
CCAATAGTTG GCATGCAGAG TAACTCTCCA TCCCAGGAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAG CAGAGCATTT TATTTAGTAA TCAGAATGCC 
ATGGCTACAA TGGCCTCCCA GAAGCAGCCA CCACCAAACA TGATATTTAG CCCAAACCAG 
AACCCGATGG CTAGTCAAGA GCAGCAGAAC CAGTCAATTT TTCACCAGCA AAGTAATATG 
GCCCCAATGA ACCAAGAGCA GCAGCCCATG CAGTTTCAGA ATCAGCCTAC GGTTTCCTCA 
CTTCAGAACC CAGGTCCTAC GCCATCTGAG TCACCACAGA CCTCCTTGTT CCATAGTTCT 
CCTCAGATCC AGTTGGTTCA AGGGTCACCT AGTTCTCAAG AGCAACAAGT AACACTCTTC 
CTCTCTCCAG CATCCATGTC TGCATTGCAA ACCAGTATAA ACCAACCAGA CATGCAGCAG 
TCTCCTCTTT ATTCCCCACA GAACAACATC CCTGGAATCC AAGGAAGCAC TTCTTCACCC 
CAGCCACAGG CTGCTTTATT TCACAACACT ACAGGAGGCA CAATAAACCA ACTACAAAAT 
TCTCCTGGCT CATCTCAGCA GACTTCAGGA ATGTTCTTAT TTGGCATTCA AAATAATTGT 
AGTCAGCTGT TAACTTCTGG GCCAGCTACA TTGCCAGAGC AGCTGATGGC CATAAATCAG 
CCGGGCCAAC CACAAAATGA GGGCCAATCT TCTGTGACAA CACTTCTTTC TCAGCAAATG 
CCAGAATCTG CCCCACTGGC CTCCTCGGTG AACAACAGTC AGAACATGGA AAAGATCGAT 
TTGCTTGTTT CGTTGCAAAG CCAAGGGAAC AATTTAACCG GCTCCTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770726.1
CDS	759..5189
Translation	MCSVFTLGLYTADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF

Target ORF information:

RefSeq Version	XM_008772504.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear factor of activated T-cells 5, tonicity-responsive (Nfat5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772504.2

ORF Insert Sequence:

ATGTGTAGTG TATTTACTCT TGGATTGTAC ACCGCAGATG CTTCTTCAGC TCCCTCCTCC 
TCCATGGGCG GTGCTTGCAG CTCCTTTACC ACCTCTTCCA GCCCTACCAT TTATTCTACC 
TCAGTCACCG ACAGCAAGGC TATGCAAGTG GAGAGCTGCT CCTCAGCCGT GGGGGTAAGT 
AACAGAGGGG TAAGTGAAAA GCAGTTAACC GGTAACACAG TTCAGCAGCA TCCATCAACC 
CCGAAGAGGC ACACAGTTTT GTACATCTCA CCACCACCTG AGGACTTGCT GGATAACAGT 
CGGATGTCCT GCCAGGATGA GGGGTGTGGA TTGGAATCTG AGCAGAGCTG CAGTATGTGG 
ATGGAGGATT CCCCCTCCAA CTTCAGTAAC ATGAGCACCA GTTCCTACAA TGATAACACT 
GAGGTACCTC GTAAATCACG AAAACGAAAT CCAAAGCAGA GGCCGGGGGT CAAACGACGA 
GATTGTGAAG AATCTAATAT GGATATATTT GATGCCGACA GTGCCAAAGC ACCTCACTAT 
GTGCTTTCTC AGCTTACCAC GGACAACAAA GGCAACTCAA AAGCTGGAAA TGGAACATTG 
GACAGCCAAA AGGGAACTGG AGTAAAGAAG AGTCCTATGT TGTGTGGACA GTATCCGGTT 
AAAAGTGAGG GGAAGGAGCT GAAGATAGTG GTACAGCCTG AAACCCAACA CCGAGCCCGG 
TACCTGACAG AGGGCAGCCG AGGCTCCGTA AAAGATAGAA CACAGCAAGG CTTTCCTACG 
GTGAAGCTGG AAGGTCATAA TGAGCCAGTG GTGTTGCAGG TGTTTGTGGG CAATGACTCT 
GGTCGAGTAA AGCCACACGG ATTCTATCAG GCCTGCAGAG TGACCGGGCG AAACACAACT 
CCGTGCAAAG AAGTGGACAT CGAAGGCACC ACTGTGATAG AGGTTGGCCT CGACCCTAGC 
AGCAACATGA CGCTGGCAGT GGACTGTGTA GGAATACTGA AGCTGAGAAA TGCTGATGTT 
GAGGCCAGAA TTGGAATTGC TGGTTCTAAG AAAAAGAGCA CTCGTGCCAG ATTGGTTTTT 
CGAGTTAATA TCACGAGGAA AGATGGCTCT ACTCTGACAT TGCAGACACC TTCTTCCCCA 
ATTTTATGCA CTCAGCCAGC AGGAGTTCCT GAGATCTTAA AGAAAAGCTT GCATAGCTGT 
TCAGTGAAAG GAGAGGAAGA AGTGTTTTTA ATTGGGAAGA ACTTTCTGAA AGGAACTAAA 
GTTATTTTCC AGGAAAATGT TTCTGATGAA AACTCTTGGA AATCAGAAGC TGAAATTGAC 
ATGGAATTGT TCCATCAGAA CCATCTTATT GTGAAAGTTC CTCCATATCA TGACCAACAT 
ATAACTTTGC CTGTATCAGT GGGAATATAT GTTGTGACCA ATGCTGGAAG ATCTCATGAT 
GTTCAGCCAT TTACTTACAC TCCAGATCCA GCAGCTGGTG CTTTGAGTGT AAATGTGAAA 
AAGGAGATAT CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AAGCCTTGAA AGCAATGAAA 
ACGACTGGCT GTAACGTAGA TAAGGTAACT ATCCTTCCTA ATGCCCTGAT CACTCCACTC 
ATATCGAGCA GCATGATTAA GACTGAAGAT GTTACTCCAA TGGAAGTAAC ATCAGAGAAA 
AGATCTTCCC CAATTTTTCA GACTACAAAG ACAGTCGGAT CAACCCAGCA AACTTTAGAA 
ACTATTTCTA ACATAGCGGG AAATGCATCC TTTTCATCAC CATCATCTTC TCACTTATCT 
CCTGAAAATG AAAATCAGCA GCAGCTTCAG CCCAAGGCAT ACAACCCGGA GACTCTGACA 
ACTATCCAAA CGCAAGACAT CTCACAGCCA GGGACTTTTC CAGCGGTTTC TGCTTCTAGT 
CAGCTGCCCA GCAGCGATGC ACTGCTGCAG CAGGCGGCAC AGTTTCAGAC AAGAGACGCT 
CAGTCCAGAG ACACAATGCA GTCAGACTCA GTGGTTAACT TGTCACAGTT GACTGAGGCA 
CCACAGCAGC AGCAGTCCCC ACTGCAAGAA CAAGCACAGA TACCATCAAA TATTTTTCCG 
TCGCCAAATA GTGTGAGCCA GCTCCAGAGT ACTATTCAGC AGCTGCAAGC AGGAAGTTTT 
ACAGGCAGCA CTGCTAGTGG CAGCAATGGA AGTGTTGACT TGGTCCAGCA GGTTTTAGAG 
GCACAACAAC AGTTATCGTC TGTTCTGTTT TCTACTCCAG ATGGTAATGA GAATGTTCAA 
GAACAGCTGA ATGCAGACAT TTTCCAAGTC AGTCAAATTC AAAATAGTGT AAGCCCTGGA 
ATGTTTTCCT CAACAGAGTC TGCAGTTCAC ACCAGACCAG ATAACTTACT ACCTGGGAGG 
GCCGACAGCG TCCATCAGCA GACTGAAAAC ACACTGTCTA GTCAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAAGTG ATAGAGTCTT CAGCTGCCAT GGTGATGGAG 
ATGCAGCAGA GCATTTGCCA AGCAGCAGCC CAGATTCAGT CAGAACTGTT TCCTTCAGCT 
GCTTCAGCAA ATGGAAGCCT TCAGCAGTCT CCAGTTTACC AGCAGCCTTC TCACATGATG 
AGCGCCCTGC CTACCAGCGA GGACATGCAA ATGCAGTGTG AATTGTTCTC TTCCCCCCCT 
GCAGTTTCTG GAAATGAAAC ATCCACCACC ACCACACCGC AGGTGGCAAC CCCCGGCTCT 
ACTATGTTTC AGCCGCCAAA TTCAGGCGAT GGAGAAGAAA CGGGAGCACA AGCAAAACAG 
ATTCAGAGCA GTGTCTTTCA GACAATGGTC CAGATGCAGC ACAGTGGGGA CAGCCAACCT 
CAAGTAAACC TCTTTTCATC TACAAAAAAC ATAATGAGTG TTCAGAGTAA TGGTACCCAG 
CAACAAGGGA ATAGCTTGTT CCAGCAGGGG AGCGAGATGC TGTCACTTCA GTCTGGGAGC 
TTCTTGCAGC AGTCTTCTCA TTCCCAGGCT CAGCTCTTTC ACCCTCAGAA CCCTATTGCT 
GATGCTCAGA GCCTTTCCCA GGAGACTCAA GGTCCAATGT TTCATAGCGC CAATCCCATC 
GTCCACAGTC AGACTTCCAC AGCATCCTCC GAACAGCTGC AGCCTTCCAT GTTTCACTCG 
CAGAGTACCA TTGCTGTACT ACAGGGCTCT TCAGTCCCTC AGGACCAGCA GTCACCCAAC 
ATATATCTTT CCCAAAGTTC CATCAGTAAT CTTCAAACTA ACACAGTAGC CCAAGAAGAG 
CAGATTTCAT TTTTTTCAGC ACAGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACACT 
GAGCAGCAAG CTGCTTTTCA ACAGCAGCCT CCAATCTCGC ACATCCAGAC CCCTCTTCTT 
TCCCAGGAAC AGGCACAGCC CTCTCAGCAA GGTTTATTTC AGCCTCAGGT GGCCCTGGGC 
TCCCTTCCTG CTAATCCAAT GCCTCAGAAC CAGCAAGGCC CAATTTTCCA GACACAGCGC 
CCAATAGTTG GCATGCAGAG TAACTCTCCA TCCCAGGAGC AGCAGCAGCA GCAACAGCAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAG CAGAGCATTT TATTTAGTAA TCAGAATGCC 
ATGGCTACAA TGGCCTCCCA GAAGCAGCCA CCACCAAACA TGATATTTAG CCCAAACCAG 
AACCCGATGG CTAGTCAAGA GCAGCAGAAC CAGTCAATTT TTCACCAGCA AAGTAATATG 
GCCCCAATGA ACCAAGAGCA GCAGCCCATG CAGTTTCAGA ATCAGCCTAC GGTTTCCTCA 
CTTCAGAACC CAGGTCCTAC GCCATCTGAG TCACCACAGA CCTCCTTGTT CCATAGTTCT 
CCTCAGATCC AGTTGGTTCA AGGGTCACCT AGTTCTCAAG AGCAACAAGT AACACTCTTC 
CTCTCTCCAG CATCCATGTC TGCATTGCAA ACCAGTATAA ACCAACCAGA CATGCAGCAG 
TCTCCTCTTT ATTCCCCACA GAACAACATC CCTGGAATCC AAGGAAGCAC TTCTTCACCC 
CAGCCACAGG CTGCTTTATT TCACAACACT ACAGGAGGCA CAATAAACCA ACTACAAAAT 
TCTCCTGGCT CATCTCAGCA GACTTCAGGA ATGTTCTTAT TTGGCATTCA AAATAATTGT 
AGTCAGCTGT TAACTTCTGG GCCAGCTACA TTGCCAGAGC AGCTGATGGC CATAAATCAG 
CCGGGCCAAC CACAAAATGA GGGCCAATCT TCTGTGACAA CACTTCTTTC TCAGCAAATG 
CCAGAATCTG CCCCACTGGC CTCCTCGGTG AACAACAGTC AGAACATGGA AAAGATCGAT 
TTGCTTGTTT CGTTGCAAAG CCAAGGGAAC AATTTAACCG GCTCCTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TNF-? promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells.
The Journal of biological chemistry292(42)17561-17575(2017 10)
Johnson ZI,Doolittle AC,Snuggs JW,Shapiro IM,Le Maitre CL,Risbud MV

Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus.
Scientific reports7(1)4543(2017 Jul)
Liu C,Choi H,Johnson ZI,Tian J,Shapiro IM,Risbud MV

NFAT5 protects astrocytes against oxygen-glucose-serum deprivation/restoration damage via the SIRT1/Nrf2 pathway.
Journal of molecular neuroscience : MN61(1)96-104(2017 Jan)
Xia X,Qu B,Li YM,Yang LB,Fan KX,Zheng H,Huang HD,Gu JW,Kuang YQ,Ma Y

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Nfat5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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