NFAT5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFAT5
Entrez Gene ID	489734
Full Name	nuclear factor of activated T cells 5
General protein information	Preferred Names nuclear factor of activated T cells 5 Names nuclear factor of activated T-cells 5 nuclear factor of activated T-cells 5, tonicity-responsive
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005620767.3	XP_005620824.1	nuclear factor of activated T-cells 5 isoform X1
XM_005620768.3	XP_005620825.1	nuclear factor of activated T-cells 5 isoform X2
XM_005620764.3	XP_005620821.1	nuclear factor of activated T-cells 5 isoform X3
XM_022419273.1	XP_022274981.1	nuclear factor of activated T-cells 5 isoform X4
XM_005620764.2	XP_005620821.1	nuclear factor of activated T-cells 5 isoform X1
XM_005620768.2	XP_005620825.1	nuclear factor of activated T-cells 5 isoform X3
XM_005620767.2	XP_005620824.1	nuclear factor of activated T-cells 5 isoform X2
XM_022419272.1	XP_022274980.1	nuclear factor of activated T-cells 5 isoform X3

Pathways from BioSystems

Homologies

	NFAT5	XP_001093880.2
Mus musculus (house mouse)	Nfat5	NP_061293.2
Xenopus tropicalis (tropical clawed frog)	nfat5	XP_002937623.2
Homo sapiens (human)	NFAT5	NP_619727.2
Pan troglodytes (chimpanzee)	NFAT5	XP_001168930.2
Canis lupus familiaris (dog)	NFAT5	XP_005620824.1
Gallus gallus (chicken)	NFAT5	NP_001185929.1
Bos taurus (cattle)	NFAT5	XP_002694885.1
Rattus norvegicus (Norway rat)	Nfat5	NP_001100895.1
Danio rerio (zebrafish)	LOC555802	XP_005174610.1

Gene Ontology

The following NFAT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFAT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe07179	XM_005620764.3 Latest version!	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe07179	XM_022419272.1 Latest version!	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe07181	XM_005620767.3 Latest version!	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe07182	XM_005620768.3 Latest version!	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe288547	XM_022419273.1 Latest version!	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCe07179	XM_005620764.2	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe07182	XM_005620768.2	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe07181	XM_005620767.2	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFAT5 ( XM_005620764.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620821.1 Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe07179
Clone ID Related Accession (Same CDS sequence)	XM_005620764.3 , XM_005620764.2 , XM_022419272.1
Accession Version	XM_005620764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620821.1
CDS	220..4596
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620764.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620764.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_005620764.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620821.1 Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07179
Clone ID Related Accession (Same CDS sequence)	XM_005620764.3 , XM_005620764.2 , XM_022419272.1
Accession Version	XM_005620764.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620821.1
CDS	179..4555
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620764.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620764.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_022419272.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274980.1 Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X4, mRNA.

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CloneID	OCe07179
Clone ID Related Accession (Same CDS sequence)	XM_005620764.3 , XM_005620764.2 , XM_022419272.1
Accession Version	XM_022419272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274980.1
CDS	312..4688
Translation	MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_022419272.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419272.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
GTCACCGACA GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC 
AGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG 
AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG 
ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCAGAGC AGAGCTGCAG TATGTGGATG 
GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG 
GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT 
TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG 
CTTTCTCAGC TTACCACGGA CAACAAAGGC AACTCAAAAG CAGGAAATGG AACATTGGAA 
AACCAAAAAG GAAGTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAGTA TCCTGTTAAA 
AGTGAGGGAA AGGAACTGAA GATAGTTGTA CAGCCTGAAA CCCAGCACCG AGCTCGGTAC 
CTGACTGAGG GTAGCCGTGG CTCAGTAAAG GACAGGACAC AACAAGGCTT TCCTACAGTA 
AAGCTGGAAG GCCATAATGA GCCAGTAGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT 
CGAGTGAAAC CACATGGATT TTATCAGGCC TGTAGGGTAA CTGGGCGAAA TACAACTCCC 
TGTAAAGAAG TGGACATTGA AGGCACTACT GTTATAGAAG TTGGTCTTGA TCCAAGCAAC 
AACATGACAC TGGCGGTGGA CTGTGTAGGA ATATTGAAAT TGAGGAATGC TGATGTTGAA 
GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAAAGCACTC GTGCCAGATT GGTTTTTAGA 
GTTAACATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCGATT 
TTATGTACTC AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA 
GTGAAAGGAG AGGAAGAAGT ATTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT 
ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGACATG 
GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA CCAACATATA 
ACGTTGCCAG TATCAGTGGG AATATATGTA GTGACCAATG CTGGAAGATC TCATGATGTT 
CAGCCATTCA CTTACACTCC TGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG 
AAAGAAATAT CTAGTCCTTC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA 
ACTACTGGTT GTAACTTAGA TAAGGTAAAT ATTCTTCCTA ATGCTCTGAT CACTCCACTC 
ATATCAAGCA GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA 
AGATCTTCCC CTATTTTTAA GACTACAAAG ACAGTTGGAT CAACTCAACA AACATTAGAA 
AATATCTCTA ACATAGCAGG AAATGGGTCT TTTTCATCAT CATCATCTTC CCACTTACCT 
TCTGAAAATG AAAAGCAGCA GCAGATTCAA CCCAAGACAT ACAACCCAGA GACCCTGACA 
ACTATCCAAA CACAGGACAT TTCACAGCCT GGTACCTTTC CAGCAGTTTC TGCTTCTAAT 
CAGCTGCCCA ACAGTGATGC ACTACTGCAG CAGGCTACCC AGTTTCAGAC CAGAGAAACT 
CCAACTAGAG AAGTACTGCA ATCAGATGGT ACAGTGGTTA ATTTGTCACA CCTGACTGAG 
TCATCCCAGC AACAGCAGCA GTCACCACTA CAAGAACAAG CGCAGACTTT ACAGCAGCAG 
ATTTCATCCA ATATTTTCCC ATCACCAAAT AGCGTGAGTC AGCTACAGAA TACAATTCAG 
CATTTGCAAG CCGGAAGTTT TACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAATGTTGAC 
TTGGTCCAAC AAGTTTTAGA GGCACAACAA CAGTTATCTT CAGTTTTATT TTCTGCTCCA 
GATGGTAATG AGAATGTTCA AGAACAGCTT AGTGCAGACA TTTTTCAACA AGTCAGTCAA 
ATTCAAAATA GTGTAAGCCC TGGAATATTT TCCTCAACGG AGCCAGCAGT CCATACTAGA 
CCAGATAATT TAATCGCTGG AAGAGCCGAA AGTGTTCACC CACCGACTGA GAACACATTA 
TCCAGTCAAC AGCAGCAACA ACAGCAGCAA CAAGTAATGG AATCATCAGC TGCGATGGTG 
ATGGAGATGC AGCAGAGTAT CTGCCAGGCA GCTGCCCAGA TCCAGTCCGA GTTATTTCCT 
TCACCTGCGT CAGCAAATGG GAACCTTCAG CAATCTCCAG TTTACCAGCA GACTTCTCAC 
ATGATGAGTG CATTATCTAC CAATGAAGAC ATGCAAATGC AGTGTGAATT GTTTTCTTCT 
CCACCTGCAG TTTCTGGAAA TGAAACTACT ACAACCACCA CACAGCAGGT TGCAACCCCT 
GGCACTACCA TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA 
AAGCAGATTC AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAGT 
CAACCTCAAG TTAACCTTTT TTCATCTACG AAAAGTATGA TGAGTGTGCA GAATAATGGT 
ACTCAGCAAC AAGGTAATGG TTTATTCCAG CAAGGTAATG AGATGATGTC CCTCCAATCT 
GGGAATTTTT TGCAACAGTC TTCTCATTCA CAGGCTCCAC TTTTTCATCC TCAGAATCCT 
ATTGCTGATG CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTATTTTTCA CAGTCCAAGT 
CCTATTGTCC ACAGTCAGAC TTCTACAACA TCCTCTGAAC AATTGCAGCC TCCAATGTTT 
CACTCTCAGA GTTCCATGGC TGTGCTGCAG GGCTCTTCTG TTCCTCAAGA CCAGCAGTCA 
GCCAACCTAT TTCTTTCCCA GACTCCAATG AATAATCTTC AAAGTAACAC AGTAGCCCAA 
GAAGAACAGA TTTCATTTTT TGCAGCTCAA AACTCAATTT CTCCACTTCA GTCAACATCT 
AACACTGAGC AGCAAGCTGC TTTCCAACAG CAGGCTCCAA TATCACACAT CCAGACCCCT 
ATGCTTTCTC AGGAACAGGC ACAACCCTCC CAGCAAGGTA TGTTCCAGCC TCAGGTGTCC 
CTGGGCTCCC TGCCTCCTAA TCCAATGCCT CAGAACCAAC AAGGACCGAT CTTCCAGTCA 
CAGCACTCAA TAGTCGCTAT CCAGAGTAAC TCTCCATCCC AGGAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAGCAGA GCATTTTGTT CAGTAATCAG 
AATACCATGG CCACAATGAC ATCTCAGAAG CAGCCACCAC CAAACATGAT GTTCAACCCA 
AGTCAAAATC CAATGGCTAA TCAGGAGCAA CAGAACCAAT CCATCTTTCA CCAACAAAAT 
AATATGGCCC CAATGAATCA AGAGCAGCAG CCCATGCAAT TTCAGAACCA GCCTACAGTT 
TCCTCTCTTC AGAACCCAGG CCCTACCCAG TCTGAGTCAC CACAGACCTC CTTGTTCCAT 
AGTTCTCCTC AGATTCAGTT GGTCCAAGGG TCACCCAGTT CGCAAGAGCA GCAAGTAACA 
CTGTTCCTCT CTCCAGCATC CATGTCTGCT TTGCAGACCA GTATGAACCA ACAAGACATG 
CAGCAGTCTC CTCTTTATTC TCCTCAGAAC AACATGCCTG GAATCCAAGG AGCCACATCT 
TCACCTCAAC CACAGGCTGC TTTATTTCAT AACACTACTG GAGGCACAAT GAACCAACTA 
CAGAATTCTC CTGGCTCATC TCAACAGACT TCTGGAATGT TCTTATTTGG CATTCAAAAT 
AACTGTAGTC AGCTTTTAAC TTCTGGACCA GCTACATTGC CAGATCAGTT GATGGCCATA 
AGTCAGCCAG GCCAACCCCA GAATGAGGGT CAGCCTCCTG TGACAACACT TCTTTCTCAG 
CAAATGCCAG AGAATTCTTC ACTAGCATCC TCTATAAATG CCAACCAGAA CATTGAAAAG 
ATTGATTTGC TTGTTTCATT GCAAAACCAA GGGAACAGCT TGACTGGCAC CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_005620767.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620824.1 Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X1, mRNA.

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CloneID	OCe07181
Clone ID Related Accession (Same CDS sequence)	XM_005620767.3 , XM_005620767.2
Accession Version	XM_005620767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620824.1
CDS	140..4603
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620767.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620767.3

ORF Insert Sequence:

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_005620767.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620824.1 Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe07181
Clone ID Related Accession (Same CDS sequence)	XM_005620767.3 , XM_005620767.2
Accession Version	XM_005620767.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620824.1
CDS	134..4597
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620767.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620767.2

ORF Insert Sequence:

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_005620768.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620825.1 Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe07182
Clone ID Related Accession (Same CDS sequence)	XM_005620768.3 , XM_005620768.2
Accession Version	XM_005620768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCT 
GGTGCTTTGA ATGTAAATGT GAAGAAAGAA ATATCTAGTC CTTCAAGACC TTGCTCTTTT 
GAAGAGGCCA TGAAAGCAAT GAAAACTACT GGTTGTAACT TAGATAAGGT AAATATTCTT 
CCTAATGCTC TGATCACTCC ACTCATATCA AGCAGTATGA TTAAGAGTGA AGATGTTACT 
CCAATGGAAG TAACAGCAGA AAAAAGATCT TCCCCTATTT TTAAGACTAC AAAGACAGTT 
GGATCAACTC AACAAACATT AGAAAATATC TCTAACATAG CAGGAAATGG GTCTTTTTCA 
TCATCATCAT CTTCCCACTT ACCTTCTGAA AATGAAAAGC AGCAGCAGAT TCAACCCAAG 
ACATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACC 
TTTCCAGCAG TTTCTGCTTC TAATCAGCTG CCCAACAGTG ATGCACTACT GCAGCAGGCT 
ACCCAGTTTC AGACCAGAGA AACTCCAACT AGAGAAGTAC TGCAATCAGA TGGTACAGTG 
GTTAATTTGT CACACCTGAC TGAGTCATCC CAGCAACAGC AGCAGTCACC ACTACAAGAA 
CAAGCGCAGA CTTTACAGCA GCAGATTTCA TCCAATATTT TCCCATCACC AAATAGCGTG 
AGTCAGCTAC AGAATACAAT TCAGCATTTG CAAGCCGGAA GTTTTACAGG CAGTACTGCT 
AGTGGCAGCA GTGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAACAGTTA 
TCTTCAGTTT TATTTTCTGC TCCAGATGGT AATGAGAATG TTCAAGAACA GCTTAGTGCA 
GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGTGTAA GCCCTGGAAT ATTTTCCTCA 
ACGGAGCCAG CAGTCCATAC TAGACCAGAT AATTTAATCG CTGGAAGAGC CGAAAGTGTT 
CACCCACCGA CTGAGAACAC ATTATCCAGT CAACAGCAGC AACAACAGCA GCAACAAGTA 
ATGGAATCAT CAGCTGCGAT GGTGATGGAG ATGCAGCAGA GTATCTGCCA GGCAGCTGCC 
CAGATCCAGT CCGAGTTATT TCCTTCACCT GCGTCAGCAA ATGGGAACCT TCAGCAATCT 
CCAGTTTACC AGCAGACTTC TCACATGATG AGTGCATTAT CTACCAATGA AGACATGCAA 
ATGCAGTGTG AATTGTTTTC TTCTCCACCT GCAGTTTCTG GAAATGAAAC TACTACAACC 
ACCACACAGC AGGTTGCAAC CCCTGGCACT ACCATGTTTC AGACATCAAG TTCAGGAGAT 
GGAGAAGAAA CTGGAGCACA AGCAAAGCAG ATTCAGAACA GTGTCTTTCA GACCATGGTC 
CAAATGCAAC ATAGTGGGGA CAGTCAACCT CAAGTTAACC TTTTTTCATC TACGAAAAGT 
ATGATGAGTG TGCAGAATAA TGGTACTCAG CAACAAGGTA ATGGTTTATT CCAGCAAGGT 
AATGAGATGA TGTCCCTCCA ATCTGGGAAT TTTTTGCAAC AGTCTTCTCA TTCACAGGCT 
CCACTTTTTC ATCCTCAGAA TCCTATTGCT GATGCTCAGA ACCTTTCCCA GGAAACTCAA 
GGTTCTATTT TTCACAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AACATCCTCT 
GAACAATTGC AGCCTCCAAT GTTTCACTCT CAGAGTTCCA TGGCTGTGCT GCAGGGCTCT 
TCTGTTCCTC AAGACCAGCA GTCAGCCAAC CTATTTCTTT CCCAGACTCC AATGAATAAT 
CTTCAAAGTA ACACAGTAGC CCAAGAAGAA CAGATTTCAT TTTTTGCAGC TCAAAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCTAACACT GAGCAGCAAG CTGCTTTCCA ACAGCAGGCT 
CCAATATCAC ACATCCAGAC CCCTATGCTT TCTCAGGAAC AGGCACAACC CTCCCAGCAA 
GGTATGTTCC AGCCTCAGGT GTCCCTGGGC TCCCTGCCTC CTAATCCAAT GCCTCAGAAC 
CAACAAGGAC CGATCTTCCA GTCACAGCAC TCAATAGTCG CTATCCAGAG TAACTCTCCA 
TCCCAGGAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAGCAG 
CAGAGCATTT TGTTCAGTAA TCAGAATACC ATGGCCACAA TGACATCTCA GAAGCAGCCA 
CCACCAAACA TGATGTTCAA CCCAAGTCAA AATCCAATGG CTAATCAGGA GCAACAGAAC 
CAATCCATCT TTCACCAACA AAATAATATG GCCCCAATGA ATCAAGAGCA GCAGCCCATG 
CAATTTCAGA ACCAGCCTAC AGTTTCCTCT CTTCAGAACC CAGGCCCTAC CCAGTCTGAG 
TCACCACAGA CCTCCTTGTT CCATAGTTCT CCTCAGATTC AGTTGGTCCA AGGGTCACCC 
AGTTCGCAAG AGCAGCAAGT AACACTGTTC CTCTCTCCAG CATCCATGTC TGCTTTGCAG 
ACCAGTATGA ACCAACAAGA CATGCAGCAG TCTCCTCTTT ATTCTCCTCA GAACAACATG 
CCTGGAATCC AAGGAGCCAC ATCTTCACCT CAACCACAGG CTGCTTTATT TCATAACACT 
ACTGGAGGCA CAATGAACCA ACTACAGAAT TCTCCTGGCT CATCTCAACA GACTTCTGGA 
ATGTTCTTAT TTGGCATTCA AAATAACTGT AGTCAGCTTT TAACTTCTGG ACCAGCTACA 
TTGCCAGATC AGTTGATGGC CATAAGTCAG CCAGGCCAAC CCCAGAATGA GGGTCAGCCT 
CCTGTGACAA CACTTCTTTC TCAGCAAATG CCAGAGAATT CTTCACTAGC ATCCTCTATA 
AATGCCAACC AGAACATTGA AAAGATTGAT TTGCTTGTTT CATTGCAAAA CCAAGGGAAC 
AGCTTGACTG GCACCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620825.1
CDS	141..4601
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620768.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620768.3

ORF Insert Sequence:

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCT 
GGTGCTTTGA ATGTAAATGT GAAGAAAGAA ATATCTAGTC CTTCAAGACC TTGCTCTTTT 
GAAGAGGCCA TGAAAGCAAT GAAAACTACT GGTTGTAACT TAGATAAGGT AAATATTCTT 
CCTAATGCTC TGATCACTCC ACTCATATCA AGCAGTATGA TTAAGAGTGA AGATGTTACT 
CCAATGGAAG TAACAGCAGA AAAAAGATCT TCCCCTATTT TTAAGACTAC AAAGACAGTT 
GGATCAACTC AACAAACATT AGAAAATATC TCTAACATAG CAGGAAATGG GTCTTTTTCA 
TCATCATCAT CTTCCCACTT ACCTTCTGAA AATGAAAAGC AGCAGCAGAT TCAACCCAAG 
ACATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACC 
TTTCCAGCAG TTTCTGCTTC TAATCAGCTG CCCAACAGTG ATGCACTACT GCAGCAGGCT 
ACCCAGTTTC AGACCAGAGA AACTCCAACT AGAGAAGTAC TGCAATCAGA TGGTACAGTG 
GTTAATTTGT CACACCTGAC TGAGTCATCC CAGCAACAGC AGCAGTCACC ACTACAAGAA 
CAAGCGCAGA CTTTACAGCA GCAGATTTCA TCCAATATTT TCCCATCACC AAATAGCGTG 
AGTCAGCTAC AGAATACAAT TCAGCATTTG CAAGCCGGAA GTTTTACAGG CAGTACTGCT 
AGTGGCAGCA GTGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAACAGTTA 
TCTTCAGTTT TATTTTCTGC TCCAGATGGT AATGAGAATG TTCAAGAACA GCTTAGTGCA 
GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGTGTAA GCCCTGGAAT ATTTTCCTCA 
ACGGAGCCAG CAGTCCATAC TAGACCAGAT AATTTAATCG CTGGAAGAGC CGAAAGTGTT 
CACCCACCGA CTGAGAACAC ATTATCCAGT CAACAGCAGC AACAACAGCA GCAACAAGTA 
ATGGAATCAT CAGCTGCGAT GGTGATGGAG ATGCAGCAGA GTATCTGCCA GGCAGCTGCC 
CAGATCCAGT CCGAGTTATT TCCTTCACCT GCGTCAGCAA ATGGGAACCT TCAGCAATCT 
CCAGTTTACC AGCAGACTTC TCACATGATG AGTGCATTAT CTACCAATGA AGACATGCAA 
ATGCAGTGTG AATTGTTTTC TTCTCCACCT GCAGTTTCTG GAAATGAAAC TACTACAACC 
ACCACACAGC AGGTTGCAAC CCCTGGCACT ACCATGTTTC AGACATCAAG TTCAGGAGAT 
GGAGAAGAAA CTGGAGCACA AGCAAAGCAG ATTCAGAACA GTGTCTTTCA GACCATGGTC 
CAAATGCAAC ATAGTGGGGA CAGTCAACCT CAAGTTAACC TTTTTTCATC TACGAAAAGT 
ATGATGAGTG TGCAGAATAA TGGTACTCAG CAACAAGGTA ATGGTTTATT CCAGCAAGGT 
AATGAGATGA TGTCCCTCCA ATCTGGGAAT TTTTTGCAAC AGTCTTCTCA TTCACAGGCT 
CCACTTTTTC ATCCTCAGAA TCCTATTGCT GATGCTCAGA ACCTTTCCCA GGAAACTCAA 
GGTTCTATTT TTCACAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AACATCCTCT 
GAACAATTGC AGCCTCCAAT GTTTCACTCT CAGAGTTCCA TGGCTGTGCT GCAGGGCTCT 
TCTGTTCCTC AAGACCAGCA GTCAGCCAAC CTATTTCTTT CCCAGACTCC AATGAATAAT 
CTTCAAAGTA ACACAGTAGC CCAAGAAGAA CAGATTTCAT TTTTTGCAGC TCAAAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCTAACACT GAGCAGCAAG CTGCTTTCCA ACAGCAGGCT 
CCAATATCAC ACATCCAGAC CCCTATGCTT TCTCAGGAAC AGGCACAACC CTCCCAGCAA 
GGTATGTTCC AGCCTCAGGT GTCCCTGGGC TCCCTGCCTC CTAATCCAAT GCCTCAGAAC 
CAACAAGGAC CGATCTTCCA GTCACAGCAC TCAATAGTCG CTATCCAGAG TAACTCTCCA 
TCCCAGGAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAGCAG 
CAGAGCATTT TGTTCAGTAA TCAGAATACC ATGGCCACAA TGACATCTCA GAAGCAGCCA 
CCACCAAACA TGATGTTCAA CCCAAGTCAA AATCCAATGG CTAATCAGGA GCAACAGAAC 
CAATCCATCT TTCACCAACA AAATAATATG GCCCCAATGA ATCAAGAGCA GCAGCCCATG 
CAATTTCAGA ACCAGCCTAC AGTTTCCTCT CTTCAGAACC CAGGCCCTAC CCAGTCTGAG 
TCACCACAGA CCTCCTTGTT CCATAGTTCT CCTCAGATTC AGTTGGTCCA AGGGTCACCC 
AGTTCGCAAG AGCAGCAAGT AACACTGTTC CTCTCTCCAG CATCCATGTC TGCTTTGCAG 
ACCAGTATGA ACCAACAAGA CATGCAGCAG TCTCCTCTTT ATTCTCCTCA GAACAACATG 
CCTGGAATCC AAGGAGCCAC ATCTTCACCT CAACCACAGG CTGCTTTATT TCATAACACT 
ACTGGAGGCA CAATGAACCA ACTACAGAAT TCTCCTGGCT CATCTCAACA GACTTCTGGA 
ATGTTCTTAT TTGGCATTCA AAATAACTGT AGTCAGCTTT TAACTTCTGG ACCAGCTACA 
TTGCCAGATC AGTTGATGGC CATAAGTCAG CCAGGCCAAC CCCAGAATGA GGGTCAGCCT 
CCTGTGACAA CACTTCTTTC TCAGCAAATG CCAGAGAATT CTTCACTAGC ATCCTCTATA 
AATGCCAACC AGAACATTGA AAAGATTGAT TTGCTTGTTT CATTGCAAAA CCAAGGGAAC 
AGCTTGACTG GCACCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_005620768.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620825.1 Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

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CloneID	OCe07182
Clone ID Related Accession (Same CDS sequence)	XM_005620768.3 , XM_005620768.2
Accession Version	XM_005620768.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCT 
GGTGCTTTGA ATGTAAATGT GAAGAAAGAA ATATCTAGTC CTTCAAGACC TTGCTCTTTT 
GAAGAGGCCA TGAAAGCAAT GAAAACTACT GGTTGTAACT TAGATAAGGT AAATATTCTT 
CCTAATGCTC TGATCACTCC ACTCATATCA AGCAGTATGA TTAAGAGTGA AGATGTTACT 
CCAATGGAAG TAACAGCAGA AAAAAGATCT TCCCCTATTT TTAAGACTAC AAAGACAGTT 
GGATCAACTC AACAAACATT AGAAAATATC TCTAACATAG CAGGAAATGG GTCTTTTTCA 
TCATCATCAT CTTCCCACTT ACCTTCTGAA AATGAAAAGC AGCAGCAGAT TCAACCCAAG 
ACATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACC 
TTTCCAGCAG TTTCTGCTTC TAATCAGCTG CCCAACAGTG ATGCACTACT GCAGCAGGCT 
ACCCAGTTTC AGACCAGAGA AACTCCAACT AGAGAAGTAC TGCAATCAGA TGGTACAGTG 
GTTAATTTGT CACACCTGAC TGAGTCATCC CAGCAACAGC AGCAGTCACC ACTACAAGAA 
CAAGCGCAGA CTTTACAGCA GCAGATTTCA TCCAATATTT TCCCATCACC AAATAGCGTG 
AGTCAGCTAC AGAATACAAT TCAGCATTTG CAAGCCGGAA GTTTTACAGG CAGTACTGCT 
AGTGGCAGCA GTGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAACAGTTA 
TCTTCAGTTT TATTTTCTGC TCCAGATGGT AATGAGAATG TTCAAGAACA GCTTAGTGCA 
GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGTGTAA GCCCTGGAAT ATTTTCCTCA 
ACGGAGCCAG CAGTCCATAC TAGACCAGAT AATTTAATCG CTGGAAGAGC CGAAAGTGTT 
CACCCACCGA CTGAGAACAC ATTATCCAGT CAACAGCAGC AACAACAGCA GCAACAAGTA 
ATGGAATCAT CAGCTGCGAT GGTGATGGAG ATGCAGCAGA GTATCTGCCA GGCAGCTGCC 
CAGATCCAGT CCGAGTTATT TCCTTCACCT GCGTCAGCAA ATGGGAACCT TCAGCAATCT 
CCAGTTTACC AGCAGACTTC TCACATGATG AGTGCATTAT CTACCAATGA AGACATGCAA 
ATGCAGTGTG AATTGTTTTC TTCTCCACCT GCAGTTTCTG GAAATGAAAC TACTACAACC 
ACCACACAGC AGGTTGCAAC CCCTGGCACT ACCATGTTTC AGACATCAAG TTCAGGAGAT 
GGAGAAGAAA CTGGAGCACA AGCAAAGCAG ATTCAGAACA GTGTCTTTCA GACCATGGTC 
CAAATGCAAC ATAGTGGGGA CAGTCAACCT CAAGTTAACC TTTTTTCATC TACGAAAAGT 
ATGATGAGTG TGCAGAATAA TGGTACTCAG CAACAAGGTA ATGGTTTATT CCAGCAAGGT 
AATGAGATGA TGTCCCTCCA ATCTGGGAAT TTTTTGCAAC AGTCTTCTCA TTCACAGGCT 
CCACTTTTTC ATCCTCAGAA TCCTATTGCT GATGCTCAGA ACCTTTCCCA GGAAACTCAA 
GGTTCTATTT TTCACAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AACATCCTCT 
GAACAATTGC AGCCTCCAAT GTTTCACTCT CAGAGTTCCA TGGCTGTGCT GCAGGGCTCT 
TCTGTTCCTC AAGACCAGCA GTCAGCCAAC CTATTTCTTT CCCAGACTCC AATGAATAAT 
CTTCAAAGTA ACACAGTAGC CCAAGAAGAA CAGATTTCAT TTTTTGCAGC TCAAAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCTAACACT GAGCAGCAAG CTGCTTTCCA ACAGCAGGCT 
CCAATATCAC ACATCCAGAC CCCTATGCTT TCTCAGGAAC AGGCACAACC CTCCCAGCAA 
GGTATGTTCC AGCCTCAGGT GTCCCTGGGC TCCCTGCCTC CTAATCCAAT GCCTCAGAAC 
CAACAAGGAC CGATCTTCCA GTCACAGCAC TCAATAGTCG CTATCCAGAG TAACTCTCCA 
TCCCAGGAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAGCAG 
CAGAGCATTT TGTTCAGTAA TCAGAATACC ATGGCCACAA TGACATCTCA GAAGCAGCCA 
CCACCAAACA TGATGTTCAA CCCAAGTCAA AATCCAATGG CTAATCAGGA GCAACAGAAC 
CAATCCATCT TTCACCAACA AAATAATATG GCCCCAATGA ATCAAGAGCA GCAGCCCATG 
CAATTTCAGA ACCAGCCTAC AGTTTCCTCT CTTCAGAACC CAGGCCCTAC CCAGTCTGAG 
TCACCACAGA CCTCCTTGTT CCATAGTTCT CCTCAGATTC AGTTGGTCCA AGGGTCACCC 
AGTTCGCAAG AGCAGCAAGT AACACTGTTC CTCTCTCCAG CATCCATGTC TGCTTTGCAG 
ACCAGTATGA ACCAACAAGA CATGCAGCAG TCTCCTCTTT ATTCTCCTCA GAACAACATG 
CCTGGAATCC AAGGAGCCAC ATCTTCACCT CAACCACAGG CTGCTTTATT TCATAACACT 
ACTGGAGGCA CAATGAACCA ACTACAGAAT TCTCCTGGCT CATCTCAACA GACTTCTGGA 
ATGTTCTTAT TTGGCATTCA AAATAACTGT AGTCAGCTTT TAACTTCTGG ACCAGCTACA 
TTGCCAGATC AGTTGATGGC CATAAGTCAG CCAGGCCAAC CCCAGAATGA GGGTCAGCCT 
CCTGTGACAA CACTTCTTTC TCAGCAAATG CCAGAGAATT CTTCACTAGC ATCCTCTATA 
AATGCCAACC AGAACATTGA AAAGATTGAT TTGCTTGTTT CATTGCAAAA CCAAGGGAAC 
AGCTTGACTG GCACCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620825.1
CDS	134..4594
Translation	MSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSALGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGSGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_005620768.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620768.2

ORF Insert Sequence:

ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT 
TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTGGAGAGC 
TGCTCCTCAG CCTTGGGGGT AAGTAACAGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC 
ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA 
CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA 
TCAGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC 
ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG 
CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC 
GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAAC 
TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA GTGGAGTAAA GAAGAGCCCT 
ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCT 
GGTGCTTTGA ATGTAAATGT GAAGAAAGAA ATATCTAGTC CTTCAAGACC TTGCTCTTTT 
GAAGAGGCCA TGAAAGCAAT GAAAACTACT GGTTGTAACT TAGATAAGGT AAATATTCTT 
CCTAATGCTC TGATCACTCC ACTCATATCA AGCAGTATGA TTAAGAGTGA AGATGTTACT 
CCAATGGAAG TAACAGCAGA AAAAAGATCT TCCCCTATTT TTAAGACTAC AAAGACAGTT 
GGATCAACTC AACAAACATT AGAAAATATC TCTAACATAG CAGGAAATGG GTCTTTTTCA 
TCATCATCAT CTTCCCACTT ACCTTCTGAA AATGAAAAGC AGCAGCAGAT TCAACCCAAG 
ACATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACC 
TTTCCAGCAG TTTCTGCTTC TAATCAGCTG CCCAACAGTG ATGCACTACT GCAGCAGGCT 
ACCCAGTTTC AGACCAGAGA AACTCCAACT AGAGAAGTAC TGCAATCAGA TGGTACAGTG 
GTTAATTTGT CACACCTGAC TGAGTCATCC CAGCAACAGC AGCAGTCACC ACTACAAGAA 
CAAGCGCAGA CTTTACAGCA GCAGATTTCA TCCAATATTT TCCCATCACC AAATAGCGTG 
AGTCAGCTAC AGAATACAAT TCAGCATTTG CAAGCCGGAA GTTTTACAGG CAGTACTGCT 
AGTGGCAGCA GTGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAACAGTTA 
TCTTCAGTTT TATTTTCTGC TCCAGATGGT AATGAGAATG TTCAAGAACA GCTTAGTGCA 
GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGTGTAA GCCCTGGAAT ATTTTCCTCA 
ACGGAGCCAG CAGTCCATAC TAGACCAGAT AATTTAATCG CTGGAAGAGC CGAAAGTGTT 
CACCCACCGA CTGAGAACAC ATTATCCAGT CAACAGCAGC AACAACAGCA GCAACAAGTA 
ATGGAATCAT CAGCTGCGAT GGTGATGGAG ATGCAGCAGA GTATCTGCCA GGCAGCTGCC 
CAGATCCAGT CCGAGTTATT TCCTTCACCT GCGTCAGCAA ATGGGAACCT TCAGCAATCT 
CCAGTTTACC AGCAGACTTC TCACATGATG AGTGCATTAT CTACCAATGA AGACATGCAA 
ATGCAGTGTG AATTGTTTTC TTCTCCACCT GCAGTTTCTG GAAATGAAAC TACTACAACC 
ACCACACAGC AGGTTGCAAC CCCTGGCACT ACCATGTTTC AGACATCAAG TTCAGGAGAT 
GGAGAAGAAA CTGGAGCACA AGCAAAGCAG ATTCAGAACA GTGTCTTTCA GACCATGGTC 
CAAATGCAAC ATAGTGGGGA CAGTCAACCT CAAGTTAACC TTTTTTCATC TACGAAAAGT 
ATGATGAGTG TGCAGAATAA TGGTACTCAG CAACAAGGTA ATGGTTTATT CCAGCAAGGT 
AATGAGATGA TGTCCCTCCA ATCTGGGAAT TTTTTGCAAC AGTCTTCTCA TTCACAGGCT 
CCACTTTTTC ATCCTCAGAA TCCTATTGCT GATGCTCAGA ACCTTTCCCA GGAAACTCAA 
GGTTCTATTT TTCACAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AACATCCTCT 
GAACAATTGC AGCCTCCAAT GTTTCACTCT CAGAGTTCCA TGGCTGTGCT GCAGGGCTCT 
TCTGTTCCTC AAGACCAGCA GTCAGCCAAC CTATTTCTTT CCCAGACTCC AATGAATAAT 
CTTCAAAGTA ACACAGTAGC CCAAGAAGAA CAGATTTCAT TTTTTGCAGC TCAAAACTCA 
ATTTCTCCAC TTCAGTCAAC ATCTAACACT GAGCAGCAAG CTGCTTTCCA ACAGCAGGCT 
CCAATATCAC ACATCCAGAC CCCTATGCTT TCTCAGGAAC AGGCACAACC CTCCCAGCAA 
GGTATGTTCC AGCCTCAGGT GTCCCTGGGC TCCCTGCCTC CTAATCCAAT GCCTCAGAAC 
CAACAAGGAC CGATCTTCCA GTCACAGCAC TCAATAGTCG CTATCCAGAG TAACTCTCCA 
TCCCAGGAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAGCAG 
CAGAGCATTT TGTTCAGTAA TCAGAATACC ATGGCCACAA TGACATCTCA GAAGCAGCCA 
CCACCAAACA TGATGTTCAA CCCAAGTCAA AATCCAATGG CTAATCAGGA GCAACAGAAC 
CAATCCATCT TTCACCAACA AAATAATATG GCCCCAATGA ATCAAGAGCA GCAGCCCATG 
CAATTTCAGA ACCAGCCTAC AGTTTCCTCT CTTCAGAACC CAGGCCCTAC CCAGTCTGAG 
TCACCACAGA CCTCCTTGTT CCATAGTTCT CCTCAGATTC AGTTGGTCCA AGGGTCACCC 
AGTTCGCAAG AGCAGCAAGT AACACTGTTC CTCTCTCCAG CATCCATGTC TGCTTTGCAG 
ACCAGTATGA ACCAACAAGA CATGCAGCAG TCTCCTCTTT ATTCTCCTCA GAACAACATG 
CCTGGAATCC AAGGAGCCAC ATCTTCACCT CAACCACAGG CTGCTTTATT TCATAACACT 
ACTGGAGGCA CAATGAACCA ACTACAGAAT TCTCCTGGCT CATCTCAACA GACTTCTGGA 
ATGTTCTTAT TTGGCATTCA AAATAACTGT AGTCAGCTTT TAACTTCTGG ACCAGCTACA 
TTGCCAGATC AGTTGATGGC CATAAGTCAG CCAGGCCAAC CCCAGAATGA GGGTCAGCCT 
CCTGTGACAA CACTTCTTTC TCAGCAAATG CCAGAGAATT CTTCACTAGC ATCCTCTATA 
AATGCCAACC AGAACATTGA AAAGATTGAT TTGCTTGTTT CATTGCAAAA CCAAGGGAAC 
AGCTTGACTG GCACCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT5 ( XM_022419273.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274981.1 Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X5, mRNA.

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CloneID	OCe288547
Clone ID Related Accession (Same CDS sequence)	XM_022419273.1
Accession Version	XM_022419273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274981.1
CDS	190..3993
Translation	MLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPSRPCSFEEAMKAMKTTGCNLDKVNILPNALITPLISSSMIKSEDVTPMEVTAEKRSSPIFKTTKTVGSTQQTLENISNIAGNGSFSSSSSSHLPSENEKQQQIQPKTYNPETLTTIQTQDISQPGTFPAVSASNQLPNSDALLQQATQFQTRETPTREVLQSDGTVVNLSHLTESSQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQHLQAGSFTGSTASGSSGNVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQNSVSPGIFSSTEPAVHTRPDNLIAGRAESVHPPTENTLSSQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSPASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETTTTTTQQVATPGTTMFQTSSSGDGEETGAQAKQIQNSVFQTMVQMQHSGDSQPQVNLFSSTKSMMSVQNNGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAPLFHPQNPIADAQNLSQETQGSIFHSPSPIVHSQTSTTSSEQLQPPMFHSQSSMAVLQGSSVPQDQQSANLFLSQTPMNNLQSNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQAPISHIQTPMLSQEQAQPSQQGMFQPQVSLGSLPPNPMPQNQQGPIFQSQHSIVAIQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNTMATMTSQKQPPPNMMFNPSQNPMANQEQQNQSIFHQQNNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSMNQQDMQQSPLYSPQNNMPGIQGATSSPQPQAALFHNTTGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSSLASSINANQNIEKIDLLVSLQNQGNSLTGTF

Target ORF information:

RefSeq Version	XM_022419273.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear factor of activated T-cells 5 (NFAT5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419273.1

ORF Insert Sequence:

ATGTTGTGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AACTGAAGAT AGTTGTACAG 
CCTGAAACCC AGCACCGAGC TCGGTACCTG ACTGAGGGTA GCCGTGGCTC AGTAAAGGAC 
AGGACACAAC AAGGCTTTCC TACAGTAAAG CTGGAAGGCC ATAATGAGCC AGTAGTGTTG 
CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGT 
AGGGTAACTG GGCGAAATAC AACTCCCTGT AAAGAAGTGG ACATTGAAGG CACTACTGTT 
ATAGAAGTTG GTCTTGATCC AAGCAACAAC ATGACACTGG CGGTGGACTG TGTAGGAATA 
TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAA 
AGCACTCGTG CCAGATTGGT TTTTAGAGTT AACATCACAA GGAAAGATGG CTCCACTTTG 
ACACTGCAAA CACCCTCTTC TCCGATTTTA TGTACTCAGC CAGCAGGAGT GCCAGAAATC 
TTAAAGAAAA GCTTGCATAG CTGTTCAGTG AAAGGAGAGG AAGAAGTATT TTTAATCGGC 
AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT 
TGGAAGTCAG AAGCTGAAAT TGACATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG 
GTTCCCCCAT ATCATGACCA ACATATAACG TTGCCAGTAT CAGTGGGAAT ATATGTAGTG 
ACCAATGCTG GAAGATCTCA TGATGTTCAG CCATTCACTT ACACTCCTGA CCCAGCAGCA 
GCTGGTGCTT TGAATGTAAA TGTGAAGAAA GAAATATCTA GTCCTTCAAG ACCTTGCTCT 
TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGTTGTA ACTTAGATAA GGTAAATATT 
CTTCCTAATG CTCTGATCAC TCCACTCATA TCAAGCAGTA TGATTAAGAG TGAAGATGTT 
ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCCCTA TTTTTAAGAC TACAAAGACA 
GTTGGATCAA CTCAACAAAC ATTAGAAAAT ATCTCTAACA TAGCAGGAAA TGGGTCTTTT 
TCATCATCAT CATCTTCCCA CTTACCTTCT GAAAATGAAA AGCAGCAGCA GATTCAACCC 
AAGACATACA ACCCAGAGAC CCTGACAACT ATCCAAACAC AGGACATTTC ACAGCCTGGT 
ACCTTTCCAG CAGTTTCTGC TTCTAATCAG CTGCCCAACA GTGATGCACT ACTGCAGCAG 
GCTACCCAGT TTCAGACCAG AGAAACTCCA ACTAGAGAAG TACTGCAATC AGATGGTACA 
GTGGTTAATT TGTCACACCT GACTGAGTCA TCCCAGCAAC AGCAGCAGTC ACCACTACAA 
GAACAAGCGC AGACTTTACA GCAGCAGATT TCATCCAATA TTTTCCCATC ACCAAATAGC 
GTGAGTCAGC TACAGAATAC AATTCAGCAT TTGCAAGCCG GAAGTTTTAC AGGCAGTACT 
GCTAGTGGCA GCAGTGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAACAG 
TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA ATGTTCAAGA ACAGCTTAGT 
GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGTG TAAGCCCTGG AATATTTTCC 
TCAACGGAGC CAGCAGTCCA TACTAGACCA GATAATTTAA TCGCTGGAAG AGCCGAAAGT 
GTTCACCCAC CGACTGAGAA CACATTATCC AGTCAACAGC AGCAACAACA GCAGCAACAA 
GTAATGGAAT CATCAGCTGC GATGGTGATG GAGATGCAGC AGAGTATCTG CCAGGCAGCT 
GCCCAGATCC AGTCCGAGTT ATTTCCTTCA CCTGCGTCAG CAAATGGGAA CCTTCAGCAA 
TCTCCAGTTT ACCAGCAGAC TTCTCACATG ATGAGTGCAT TATCTACCAA TGAAGACATG 
CAAATGCAGT GTGAATTGTT TTCTTCTCCA CCTGCAGTTT CTGGAAATGA AACTACTACA 
ACCACCACAC AGCAGGTTGC AACCCCTGGC ACTACCATGT TTCAGACATC AAGTTCAGGA 
GATGGAGAAG AAACTGGAGC ACAAGCAAAG CAGATTCAGA ACAGTGTCTT TCAGACCATG 
GTCCAAATGC AACATAGTGG GGACAGTCAA CCTCAAGTTA ACCTTTTTTC ATCTACGAAA 
AGTATGATGA GTGTGCAGAA TAATGGTACT CAGCAACAAG GTAATGGTTT ATTCCAGCAA 
GGTAATGAGA TGATGTCCCT CCAATCTGGG AATTTTTTGC AACAGTCTTC TCATTCACAG 
GCTCCACTTT TTCATCCTCA GAATCCTATT GCTGATGCTC AGAACCTTTC CCAGGAAACT 
CAAGGTTCTA TTTTTCACAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAACATCC 
TCTGAACAAT TGCAGCCTCC AATGTTTCAC TCTCAGAGTT CCATGGCTGT GCTGCAGGGC 
TCTTCTGTTC CTCAAGACCA GCAGTCAGCC AACCTATTTC TTTCCCAGAC TCCAATGAAT 
AATCTTCAAA GTAACACAGT AGCCCAAGAA GAACAGATTT CATTTTTTGC AGCTCAAAAC 
TCAATTTCTC CACTTCAGTC AACATCTAAC ACTGAGCAGC AAGCTGCTTT CCAACAGCAG 
GCTCCAATAT CACACATCCA GACCCCTATG CTTTCTCAGG AACAGGCACA ACCCTCCCAG 
CAAGGTATGT TCCAGCCTCA GGTGTCCCTG GGCTCCCTGC CTCCTAATCC AATGCCTCAG 
AACCAACAAG GACCGATCTT CCAGTCACAG CACTCAATAG TCGCTATCCA GAGTAACTCT 
CCATCCCAGG AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGAGCA TTTTGTTCAG TAATCAGAAT ACCATGGCCA CAATGACATC TCAGAAGCAG 
CCACCACCAA ACATGATGTT CAACCCAAGT CAAAATCCAA TGGCTAATCA GGAGCAACAG 
AACCAATCCA TCTTTCACCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC 
ATGCAATTTC AGAACCAGCC TACAGTTTCC TCTCTTCAGA ACCCAGGCCC TACCCAGTCT 
GAGTCACCAC AGACCTCCTT GTTCCATAGT TCTCCTCAGA TTCAGTTGGT CCAAGGGTCA 
CCCAGTTCGC AAGAGCAGCA AGTAACACTG TTCCTCTCTC CAGCATCCAT GTCTGCTTTG 
CAGACCAGTA TGAACCAACA AGACATGCAG CAGTCTCCTC TTTATTCTCC TCAGAACAAC 
ATGCCTGGAA TCCAAGGAGC CACATCTTCA CCTCAACCAC AGGCTGCTTT ATTTCATAAC 
ACTACTGGAG GCACAATGAA CCAACTACAG AATTCTCCTG GCTCATCTCA ACAGACTTCT 
GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT 
ACATTGCCAG ATCAGTTGAT GGCCATAAGT CAGCCAGGCC AACCCCAGAA TGAGGGTCAG 
CCTCCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTTCACT AGCATCCTCT 
ATAAATGCCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG 
AACAGCTTGA CTGGCACCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NFAT5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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