nfat5b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021478038.1	XP_021333713.1	nuclear factor of activated T-cells 5 isoform X2
XM_021478039.1	XP_021333714.1	nuclear factor of activated T-cells 5 isoform X3
XM_021478040.1	XP_021333715.1	nuclear factor of activated T-cells 5 isoform X6
XM_009303606.2	XP_009301881.1	nuclear factor of activated T-cells 5 isoform X2
XM_009303607.2	XP_009301882.1	nuclear factor of activated T-cells 5 isoform X3
XM_009303605.3	XP_009301880.1	nuclear factor of activated T-cells 5 isoform X4
XM_009303608.2	XP_009301883.1	nuclear factor of activated T-cells 5 isoform X4
XM_009303607.3	XP_009301882.1	nuclear factor of activated T-cells 5 isoform X5
XM_009303605.2	XP_009301880.1	nuclear factor of activated T-cells 5 isoform X1
XM_021478037.1	XP_021333712.1	nuclear factor of activated T-cells 5 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143562	XM_021478037.1 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa143563	XM_021478038.1 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa143564	XM_021478039.1 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa143565	XM_021478040.1 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa42239	XM_009303605.3 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa42240	XM_009303606.2 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa42241	XM_009303607.3 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa42242	XM_009303608.2 Latest version!	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa42241	XM_009303607.2	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa42239	XM_009303605.2	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

nfat5b ( XM_021478037.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333712.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143562
Clone ID Related Accession (Same CDS sequence)	XM_021478037.1
Accession Version	XM_021478037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGC AGATGATAAG 
TCCTGGAAGG CAGAGGCTGA CATTGACATG GAGTTTTTCC ATCAGAATCA TCTTATTGTC 
AAAGTCCCAC CATATCAAAA TCCAGCTATC ACATCTCCTA TATCTGTTGG TATCTATGTT 
GTCACGAATG CTGGGCGTTC ACATGATGTT CAACAATTTA CATACACTCC AGAGCCAGTG 
GTTGATTCTT CTGTAAAAAA GGAGGACTTG CCTCCAGTGA CACCTTGTTG TTTTGAACAA 
ATTAAAGTAA TTGATACTGC CGTGATGACA CCAGGCTTAC CTCTTGTGAA ACGTGAAGAG 
GCCACTCCGA TGGAGGTCTC CGGTAACCCT TCGGACTTCA AGCAGACCTC TGAAGTCATC 
AATTCAGCCC AGCAAGCTTT AGACATGAGT ACTGAGATTT CCACCAACAA CTGCAAATTT 
TCCAGTCCGT TGGCACACCC AACAAGAGAG CCAGATCAAT CTCAGAATTC AGTCTTTTCC 
AACAAAGAAC CTTTCAACAC CATCCAGGAA CAGAATCTTG CATCCAGTGG CTCTTTCCAT 
GTGCCTAATA AATCTCTTCT CCAGCAAGGA GTTCAGCCAT TCCTCCTGGA ACACAGAGAC 
CGTCTTGGAC AGGAGATGCC AGGCAGCAGT GGCAGTTCAG TAGTGGCTCT TTCCCAATTA 
GATGACAATC CGCAGCAACA GCTCTCTCTT CTACCTCCAG ATGAGGTGGC CCAACTGGAG 
CAGGCCGTGC GGCAGCTGCA AGCGAAAGGA TACTGCGGCA TACAACTCCA GAGTGAGAAT 
TCACTGTCAA AGCAAAGACA ACATCAGCTT CAACAGCAGC AACAGCAAAT CCAACAACAG 
CAGCATCAAA TCCACCAACA ACAGCAGCAA ATTCAGCAAC AGCAGCAGCA CATCCAGCAT 
CAAGAGCAGC AAATCCAGCT GCAGCAGCAG CACCACCACC AGCAGCAAGC TCTGGAGAAC 
CTTCAACATC AGCTTTTTCA GCCCCAGGTT CAAATTCACT GTAGTTCAGA GCAGCAGAGC 
TCTTCCCAGG GTATGGTGCA GAATGGAAGT TCCCTTTTCC AGTCCACCCC ATCCCAACAA 
CAACAGCAGC AGCAAGCAAC TTTGTTCCAA CAAGGAGCTG GCTTTCTTCA GCAGCAACCC 
AGTCATTCCT CTCCTTCCAT GTTCCACAAC ACTACAGCAA TGACCGAAGC TCAAAGCCCT 
CAGGAGTCTC TTTTCCACAC ACAAAAGACC TCTCCCAACC AGGATCAGGT CCAAGCTTCC 
ATTTTCCAAA GCAATCTTTC GGTTCTCAGT GGGTCCAACA TGTCTTCAGA AGCTCAGCCC 
TCTGCAGCTA GTATGTTTCT TCCCCAGAAC ACAGTACCAG CTCAACTTTC CGTTAACGCC 
AACCAGCCAC AGCAACTGGC GTTCCTCACT TCCATGCAGA CATCTCTTGA GGCGCCATCT 
GTATTTCAGA CTCCAACCCA ACTGTCCCCC ATTCAGCAAG GCACTCCGAT GGATCAGCAG 
CAGTCCCCAC AGCATGCCCA ACCGGGTTCC ATGTTCCAGA GCATCTCCCA ATCACCAAGC 
AAACTCACCC CTGGTCAACA GCAGCAGGCT GGTCTACTCC TCGGATCCTT AAACTCTCCA 
ATCACACAAG AGCAATCCTC CAATCTGTTA TTCGGTGGCC AAGTACAGAT AGGATCAATG 
AACAATAGCA GCCTAGCTTC GTCAGAGCAA CAGAACACTC CCCTTCCCTT CGCTCAGGCT 
GGTCTGGTCA CCGTGAGGCA GCAGGAGGCA TCTGAACCAA TGACATTTCA GGATCAGACA 
GCAATGGTGG TCAACCTGTC ATCAAATAAG CCCATCTTCC AAGACCAGCA GCCAATGCAG 
TTGGCACCAA GTTCTAATCC AGGCTCTTCA CAGTCTGTAA ATCTTTTCAT GGCACAGTCC 
AACATGCAAG GGGGGATGAC TACACAAGAA GCTCTTTTTG CACCACAAGG AGATGTAGGT 
GGCATTCAGA CAAGCACTTC CTCTCCAGTG CAACAGCCTG GTCAACTCTT TCAGACCACA 
ATGAGTGGGA GTCTCAACCA GCCCAACCAG GATCAGCAGA CAGACCTATT CCTCTTCGAG 
ATCCAGAATG AGTGCCGTCA ACTCATGAAC AGCCAAGGAT CTACACTGTC TAATCAAATA 
ATTACCATCA ATCAGCCTGG ACAGAGTCAG CAAGAGAGTG ATGGGCAAAT CCAGTCTTTA 
CTTGGGCAGG CTATGCCAGA CCCTGGAGCT GTGCAGAGTA CCTTGACAAC CTCTCAGAAC 
ATGGAGAAAA TTGATGACCT TCTGGTGAGC CTTCAGGAGC AAGGCAATAA CATCTCCCAT 
TCCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333712.1
CDS	261..4049
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPCLCSVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_021478037.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478037.1

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGC AGATGATAAG 
TCCTGGAAGG CAGAGGCTGA CATTGACATG GAGTTTTTCC ATCAGAATCA TCTTATTGTC 
AAAGTCCCAC CATATCAAAA TCCAGCTATC ACATCTCCTA TATCTGTTGG TATCTATGTT 
GTCACGAATG CTGGGCGTTC ACATGATGTT CAACAATTTA CATACACTCC AGAGCCAGTG 
GTTGATTCTT CTGTAAAAAA GGAGGACTTG CCTCCAGTGA CACCTTGTTG TTTTGAACAA 
ATTAAAGTAA TTGATACTGC CGTGATGACA CCAGGCTTAC CTCTTGTGAA ACGTGAAGAG 
GCCACTCCGA TGGAGGTCTC CGGTAACCCT TCGGACTTCA AGCAGACCTC TGAAGTCATC 
AATTCAGCCC AGCAAGCTTT AGACATGAGT ACTGAGATTT CCACCAACAA CTGCAAATTT 
TCCAGTCCGT TGGCACACCC AACAAGAGAG CCAGATCAAT CTCAGAATTC AGTCTTTTCC 
AACAAAGAAC CTTTCAACAC CATCCAGGAA CAGAATCTTG CATCCAGTGG CTCTTTCCAT 
GTGCCTAATA AATCTCTTCT CCAGCAAGGA GTTCAGCCAT TCCTCCTGGA ACACAGAGAC 
CGTCTTGGAC AGGAGATGCC AGGCAGCAGT GGCAGTTCAG TAGTGGCTCT TTCCCAATTA 
GATGACAATC CGCAGCAACA GCTCTCTCTT CTACCTCCAG ATGAGGTGGC CCAACTGGAG 
CAGGCCGTGC GGCAGCTGCA AGCGAAAGGA TACTGCGGCA TACAACTCCA GAGTGAGAAT 
TCACTGTCAA AGCAAAGACA ACATCAGCTT CAACAGCAGC AACAGCAAAT CCAACAACAG 
CAGCATCAAA TCCACCAACA ACAGCAGCAA ATTCAGCAAC AGCAGCAGCA CATCCAGCAT 
CAAGAGCAGC AAATCCAGCT GCAGCAGCAG CACCACCACC AGCAGCAAGC TCTGGAGAAC 
CTTCAACATC AGCTTTTTCA GCCCCAGGTT CAAATTCACT GTAGTTCAGA GCAGCAGAGC 
TCTTCCCAGG GTATGGTGCA GAATGGAAGT TCCCTTTTCC AGTCCACCCC ATCCCAACAA 
CAACAGCAGC AGCAAGCAAC TTTGTTCCAA CAAGGAGCTG GCTTTCTTCA GCAGCAACCC 
AGTCATTCCT CTCCTTCCAT GTTCCACAAC ACTACAGCAA TGACCGAAGC TCAAAGCCCT 
CAGGAGTCTC TTTTCCACAC ACAAAAGACC TCTCCCAACC AGGATCAGGT CCAAGCTTCC 
ATTTTCCAAA GCAATCTTTC GGTTCTCAGT GGGTCCAACA TGTCTTCAGA AGCTCAGCCC 
TCTGCAGCTA GTATGTTTCT TCCCCAGAAC ACAGTACCAG CTCAACTTTC CGTTAACGCC 
AACCAGCCAC AGCAACTGGC GTTCCTCACT TCCATGCAGA CATCTCTTGA GGCGCCATCT 
GTATTTCAGA CTCCAACCCA ACTGTCCCCC ATTCAGCAAG GCACTCCGAT GGATCAGCAG 
CAGTCCCCAC AGCATGCCCA ACCGGGTTCC ATGTTCCAGA GCATCTCCCA ATCACCAAGC 
AAACTCACCC CTGGTCAACA GCAGCAGGCT GGTCTACTCC TCGGATCCTT AAACTCTCCA 
ATCACACAAG AGCAATCCTC CAATCTGTTA TTCGGTGGCC AAGTACAGAT AGGATCAATG 
AACAATAGCA GCCTAGCTTC GTCAGAGCAA CAGAACACTC CCCTTCCCTT CGCTCAGGCT 
GGTCTGGTCA CCGTGAGGCA GCAGGAGGCA TCTGAACCAA TGACATTTCA GGATCAGACA 
GCAATGGTGG TCAACCTGTC ATCAAATAAG CCCATCTTCC AAGACCAGCA GCCAATGCAG 
TTGGCACCAA GTTCTAATCC AGGCTCTTCA CAGTCTGTAA ATCTTTTCAT GGCACAGTCC 
AACATGCAAG GGGGGATGAC TACACAAGAA GCTCTTTTTG CACCACAAGG AGATGTAGGT 
GGCATTCAGA CAAGCACTTC CTCTCCAGTG CAACAGCCTG GTCAACTCTT TCAGACCACA 
ATGAGTGGGA GTCTCAACCA GCCCAACCAG GATCAGCAGA CAGACCTATT CCTCTTCGAG 
ATCCAGAATG AGTGCCGTCA ACTCATGAAC AGCCAAGGAT CTACACTGTC TAATCAAATA 
ATTACCATCA ATCAGCCTGG ACAGAGTCAG CAAGAGAGTG ATGGGCAAAT CCAGTCTTTA 
CTTGGGCAGG CTATGCCAGA CCCTGGAGCT GTGCAGAGTA CCTTGACAAC CTCTCAGAAC 
ATGGAGAAAA TTGATGACCT TCTGGTGAGC CTTCAGGAGC AAGGCAATAA CATCTCCCAT 
TCCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_021478038.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333713.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa143563
Clone ID Related Accession (Same CDS sequence)	XM_021478038.1
Accession Version	XM_021478038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGC AGATGATAAG 
TCCTGGAAGG CAGAGGCTGA CATTGACATG GAGTTTTTCC ATCAGAATCA TCTTATTGTC 
AAAGTCCCAC CATATCAAAA TCCAGCTATC ACATCTCCTA TATCTGTTGG TATCTATGTT 
GTCACGAATG CTGGGCGTTC ACATGATGTT CAACAATTTA CATACACTCC AGAGCCAGTG 
GTTGATTCTT CTGTAAAAAA GGAGGACTTG CCTCCAGTGA CACCTTGTTG TTTTGAACAA 
ATTAAAGTAA TTGATACTGC CGTGATGACA CCAGGCTTAC CTCTTGTGAA ACGTGAAGAG 
GCCACTCCGA TGGAGGTCTC CGGTAACCCT TCGGACTTCA AGACCTCTGA AGTCATCAAT 
TCAGCCCAGC AAGCTTTAGA CATGAGTACT GAGATTTCCA CCAACAACTG CAAATTTTCC 
AGTCCGTTGG CACACCCAAC AAGAGAGCCA GATCAATCTC AGAATTCAGT CTTTTCCAAC 
AAAGAACCTT TCAACACCAT CCAGGAACAG AATCTTGCAT CCAGTGGCTC TTTCCATGTG 
CCTAATAAAT CTCTTCTCCA GCAAGGAGTT CAGCCATTCC TCCTGGAACA CAGAGACCGT 
CTTGGACAGG AGATGCCAGG CAGCAGTGGC AGTTCAGTAG TGGCTCTTTC CCAATTAGAT 
GACAATCCGC AGCAACAGCT CTCTCTTCTA CCTCCAGATG AGGTGGCCCA ACTGGAGCAG 
GCCGTGCGGC AGCTGCAAGC GAAAGGATAC TGCGGCATAC AACTCCAGAG TGAGAATTCA 
CTGTCAAAGC AAAGACAACA TCAGCTTCAA CAGCAGCAAC AGCAAATCCA ACAACAGCAG 
CATCAAATCC ACCAACAACA GCAGCAAATT CAGCAACAGC AGCAGCACAT CCAGCATCAA 
GAGCAGCAAA TCCAGCTGCA GCAGCAGCAC CACCACCAGC AGCAAGCTCT GGAGAACCTT 
CAACATCAGC TTTTTCAGCC CCAGGTTCAA ATTCACTGTA GTTCAGAGCA GCAGAGCTCT 
TCCCAGGGTA TGGTGCAGAA TGGAAGTTCC CTTTTCCAGT CCACCCCATC CCAACAACAA 
CAGCAGCAGC AAGCAACTTT GTTCCAACAA GGAGCTGGCT TTCTTCAGCA GCAACCCAGT 
CATTCCTCTC CTTCCATGTT CCACAACACT ACAGCAATGA CCGAAGCTCA AAGCCCTCAG 
GAGTCTCTTT TCCACACACA AAAGACCTCT CCCAACCAGG ATCAGGTCCA AGCTTCCATT 
TTCCAAAGCA ATCTTTCGGT TCTCAGTGGG TCCAACATGT CTTCAGAAGC TCAGCCCTCT 
GCAGCTAGTA TGTTTCTTCC CCAGAACACA GTACCAGCTC AACTTTCCGT TAACGCCAAC 
CAGCCACAGC AACTGGCGTT CCTCACTTCC ATGCAGACAT CTCTTGAGGC GCCATCTGTA 
TTTCAGACTC CAACCCAACT GTCCCCCATT CAGCAAGGCA CTCCGATGGA TCAGCAGCAG 
TCCCCACAGC ATGCCCAACC GGGTTCCATG TTCCAGAGCA TCTCCCAATC ACCAAGCAAA 
CTCACCCCTG GTCAACAGCA GCAGGCTGGT CTACTCCTCG GATCCTTAAA CTCTCCAATC 
ACACAAGAGC AATCCTCCAA TCTGTTATTC GGTGGCCAAG TACAGATAGG ATCAATGAAC 
AATAGCAGCC TAGCTTCGTC AGAGCAACAG AACACTCCCC TTCCCTTCGC TCAGGCTGGT 
CTGGTCACCG TGAGGCAGCA GGAGGCATCT GAACCAATGA CATTTCAGGA TCAGACAGCA 
ATGGTGGTCA ACCTGTCATC AAATAAGCCC ATCTTCCAAG ACCAGCAGCC AATGCAGTTG 
GCACCAAGTT CTAATCCAGG CTCTTCACAG TCTGTAAATC TTTTCATGGC ACAGTCCAAC 
ATGCAAGGGG GGATGACTAC ACAAGAAGCT CTTTTTGCAC CACAAGGAGA TGTAGGTGGC 
ATTCAGACAA GCACTTCCTC TCCAGTGCAA CAGCCTGGTC AACTCTTTCA GACCACAATG 
AGTGGGAGTC TCAACCAGCC CAACCAGGAT CAGCAGACAG ACCTATTCCT CTTCGAGATC 
CAGAATGAGT GCCGTCAACT CATGAACAGC CAAGGATCTA CACTGTCTAA TCAAATAATT 
ACCATCAATC AGCCTGGACA GAGTCAGCAA GAGAGTGATG GGCAAATCCA GTCTTTACTT 
GGGCAGGCTA TGCCAGACCC TGGAGCTGTG CAGAGTACCT TGACAACCTC TCAGAACATG 
GAGAAAATTG ATGACCTTCT GGTGAGCCTT CAGGAGCAAG GCAATAACAT CTCCCATTCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333713.1
CDS	261..4046
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPCLCSVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_021478038.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478038.1

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGC AGATGATAAG 
TCCTGGAAGG CAGAGGCTGA CATTGACATG GAGTTTTTCC ATCAGAATCA TCTTATTGTC 
AAAGTCCCAC CATATCAAAA TCCAGCTATC ACATCTCCTA TATCTGTTGG TATCTATGTT 
GTCACGAATG CTGGGCGTTC ACATGATGTT CAACAATTTA CATACACTCC AGAGCCAGTG 
GTTGATTCTT CTGTAAAAAA GGAGGACTTG CCTCCAGTGA CACCTTGTTG TTTTGAACAA 
ATTAAAGTAA TTGATACTGC CGTGATGACA CCAGGCTTAC CTCTTGTGAA ACGTGAAGAG 
GCCACTCCGA TGGAGGTCTC CGGTAACCCT TCGGACTTCA AGACCTCTGA AGTCATCAAT 
TCAGCCCAGC AAGCTTTAGA CATGAGTACT GAGATTTCCA CCAACAACTG CAAATTTTCC 
AGTCCGTTGG CACACCCAAC AAGAGAGCCA GATCAATCTC AGAATTCAGT CTTTTCCAAC 
AAAGAACCTT TCAACACCAT CCAGGAACAG AATCTTGCAT CCAGTGGCTC TTTCCATGTG 
CCTAATAAAT CTCTTCTCCA GCAAGGAGTT CAGCCATTCC TCCTGGAACA CAGAGACCGT 
CTTGGACAGG AGATGCCAGG CAGCAGTGGC AGTTCAGTAG TGGCTCTTTC CCAATTAGAT 
GACAATCCGC AGCAACAGCT CTCTCTTCTA CCTCCAGATG AGGTGGCCCA ACTGGAGCAG 
GCCGTGCGGC AGCTGCAAGC GAAAGGATAC TGCGGCATAC AACTCCAGAG TGAGAATTCA 
CTGTCAAAGC AAAGACAACA TCAGCTTCAA CAGCAGCAAC AGCAAATCCA ACAACAGCAG 
CATCAAATCC ACCAACAACA GCAGCAAATT CAGCAACAGC AGCAGCACAT CCAGCATCAA 
GAGCAGCAAA TCCAGCTGCA GCAGCAGCAC CACCACCAGC AGCAAGCTCT GGAGAACCTT 
CAACATCAGC TTTTTCAGCC CCAGGTTCAA ATTCACTGTA GTTCAGAGCA GCAGAGCTCT 
TCCCAGGGTA TGGTGCAGAA TGGAAGTTCC CTTTTCCAGT CCACCCCATC CCAACAACAA 
CAGCAGCAGC AAGCAACTTT GTTCCAACAA GGAGCTGGCT TTCTTCAGCA GCAACCCAGT 
CATTCCTCTC CTTCCATGTT CCACAACACT ACAGCAATGA CCGAAGCTCA AAGCCCTCAG 
GAGTCTCTTT TCCACACACA AAAGACCTCT CCCAACCAGG ATCAGGTCCA AGCTTCCATT 
TTCCAAAGCA ATCTTTCGGT TCTCAGTGGG TCCAACATGT CTTCAGAAGC TCAGCCCTCT 
GCAGCTAGTA TGTTTCTTCC CCAGAACACA GTACCAGCTC AACTTTCCGT TAACGCCAAC 
CAGCCACAGC AACTGGCGTT CCTCACTTCC ATGCAGACAT CTCTTGAGGC GCCATCTGTA 
TTTCAGACTC CAACCCAACT GTCCCCCATT CAGCAAGGCA CTCCGATGGA TCAGCAGCAG 
TCCCCACAGC ATGCCCAACC GGGTTCCATG TTCCAGAGCA TCTCCCAATC ACCAAGCAAA 
CTCACCCCTG GTCAACAGCA GCAGGCTGGT CTACTCCTCG GATCCTTAAA CTCTCCAATC 
ACACAAGAGC AATCCTCCAA TCTGTTATTC GGTGGCCAAG TACAGATAGG ATCAATGAAC 
AATAGCAGCC TAGCTTCGTC AGAGCAACAG AACACTCCCC TTCCCTTCGC TCAGGCTGGT 
CTGGTCACCG TGAGGCAGCA GGAGGCATCT GAACCAATGA CATTTCAGGA TCAGACAGCA 
ATGGTGGTCA ACCTGTCATC AAATAAGCCC ATCTTCCAAG ACCAGCAGCC AATGCAGTTG 
GCACCAAGTT CTAATCCAGG CTCTTCACAG TCTGTAAATC TTTTCATGGC ACAGTCCAAC 
ATGCAAGGGG GGATGACTAC ACAAGAAGCT CTTTTTGCAC CACAAGGAGA TGTAGGTGGC 
ATTCAGACAA GCACTTCCTC TCCAGTGCAA CAGCCTGGTC AACTCTTTCA GACCACAATG 
AGTGGGAGTC TCAACCAGCC CAACCAGGAT CAGCAGACAG ACCTATTCCT CTTCGAGATC 
CAGAATGAGT GCCGTCAACT CATGAACAGC CAAGGATCTA CACTGTCTAA TCAAATAATT 
ACCATCAATC AGCCTGGACA GAGTCAGCAA GAGAGTGATG GGCAAATCCA GTCTTTACTT 
GGGCAGGCTA TGCCAGACCC TGGAGCTGTG CAGAGTACCT TGACAACCTC TCAGAACATG 
GAGAAAATTG ATGACCTTCT GGTGAGCCTT CAGGAGCAAG GCAATAACAT CTCCCATTCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_021478039.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333714.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.

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CloneID	ODa143564
Clone ID Related Accession (Same CDS sequence)	XM_021478039.1
Accession Version	XM_021478039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGA TGATAAGTCC 
TGGAAGGCAG AGGCTGACAT TGACATGGAG TTTTTCCATC AGAATCATCT TATTGTCAAA 
GTCCCACCAT ATCAAAATCC AGCTATCACA TCTCCTATAT CTGTTGGTAT CTATGTTGTC 
ACGAATGCTG GGCGTTCACA TGATGTTCAA CAATTTACAT ACACTCCAGA GCCAGTGGTT 
GATTCTTCTG TAAAAAAGGA GGACTTGCCT CCAGTGACAC CTTGTTGTTT TGAACAAATT 
AAAGTAATTG ATACTGCCGT GATGACACCA GGCTTACCTC TTGTGAAACG TGAAGAGGCC 
ACTCCGATGG AGGTCTCCGG TAACCCTTCG GACTTCAAGC AGACCTCTGA AGTCATCAAT 
TCAGCCCAGC AAGCTTTAGA CATGAGTACT GAGATTTCCA CCAACAACTG CAAATTTTCC 
AGTCCGTTGG CACACCCAAC AAGAGAGCCA GATCAATCTC AGAATTCAGT CTTTTCCAAC 
AAAGAACCTT TCAACACCAT CCAGGAACAG AATCTTGCAT CCAGTGGCTC TTTCCATGTG 
CCTAATAAAT CTCTTCTCCA GCAAGGAGTT CAGCCATTCC TCCTGGAACA CAGAGACCGT 
CTTGGACAGG AGATGCCAGG CAGCAGTGGC AGTTCAGTAG TGGCTCTTTC CCAATTAGAT 
GACAATCCGC AGCAACAGCT CTCTCTTCTA CCTCCAGATG AGGTGGCCCA ACTGGAGCAG 
GCCGTGCGGC AGCTGCAAGC GAAAGGATAC TGCGGCATAC AACTCCAGAG TGAGAATTCA 
CTGTCAAAGC AAAGACAACA TCAGCTTCAA CAGCAGCAAC AGCAAATCCA ACAACAGCAG 
CATCAAATCC ACCAACAACA GCAGCAAATT CAGCAACAGC AGCAGCACAT CCAGCATCAA 
GAGCAGCAAA TCCAGCTGCA GCAGCAGCAC CACCACCAGC AGCAAGCTCT GGAGAACCTT 
CAACATCAGC TTTTTCAGCC CCAGGTTCAA ATTCACTGTA GTTCAGAGCA GCAGAGCTCT 
TCCCAGGGTA TGGTGCAGAA TGGAAGTTCC CTTTTCCAGT CCACCCCATC CCAACAACAA 
CAGCAGCAGC AAGCAACTTT GTTCCAACAA GGAGCTGGCT TTCTTCAGCA GCAACCCAGT 
CATTCCTCTC CTTCCATGTT CCACAACACT ACAGCAATGA CCGAAGCTCA AAGCCCTCAG 
GAGTCTCTTT TCCACACACA AAAGACCTCT CCCAACCAGG ATCAGGTCCA AGCTTCCATT 
TTCCAAAGCA ATCTTTCGGT TCTCAGTGGG TCCAACATGT CTTCAGAAGC TCAGCCCTCT 
GCAGCTAGTA TGTTTCTTCC CCAGAACACA GTACCAGCTC AACTTTCCGT TAACGCCAAC 
CAGCCACAGC AACTGGCGTT CCTCACTTCC ATGCAGACAT CTCTTGAGGC GCCATCTGTA 
TTTCAGACTC CAACCCAACT GTCCCCCATT CAGCAAGGCA CTCCGATGGA TCAGCAGCAG 
TCCCCACAGC ATGCCCAACC GGGTTCCATG TTCCAGAGCA TCTCCCAATC ACCAAGCAAA 
CTCACCCCTG GTCAACAGCA GCAGGCTGGT CTACTCCTCG GATCCTTAAA CTCTCCAATC 
ACACAAGAGC AATCCTCCAA TCTGTTATTC GGTGGCCAAG TACAGATAGG ATCAATGAAC 
AATAGCAGCC TAGCTTCGTC AGAGCAACAG AACACTCCCC TTCCCTTCGC TCAGGCTGGT 
CTGGTCACCG TGAGGCAGCA GGAGGCATCT GAACCAATGA CATTTCAGGA TCAGACAGCA 
ATGGTGGTCA ACCTGTCATC AAATAAGCCC ATCTTCCAAG ACCAGCAGCC AATGCAGTTG 
GCACCAAGTT CTAATCCAGG CTCTTCACAG TCTGTAAATC TTTTCATGGC ACAGTCCAAC 
ATGCAAGGGG GGATGACTAC ACAAGAAGCT CTTTTTGCAC CACAAGGAGA TGTAGGTGGC 
ATTCAGACAA GCACTTCCTC TCCAGTGCAA CAGCCTGGTC AACTCTTTCA GACCACAATG 
AGTGGGAGTC TCAACCAGCC CAACCAGGAT CAGCAGACAG ACCTATTCCT CTTCGAGATC 
CAGAATGAGT GCCGTCAACT CATGAACAGC CAAGGATCTA CACTGTCTAA TCAAATAATT 
ACCATCAATC AGCCTGGACA GAGTCAGCAA GAGAGTGATG GGCAAATCCA GTCTTTACTT 
GGGCAGGCTA TGCCAGACCC TGGAGCTGTG CAGAGTACCT TGACAACCTC TCAGAACATG 
GAGAAAATTG ATGACCTTCT GGTGAGCCTT CAGGAGCAAG GCAATAACAT CTCCCATTCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333714.1
CDS	262..4047
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPCLCSVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASDDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_021478039.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478039.1

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCG TGTCTCTGCA GTGTGGACTG TGTGGGGATC 
CTGAAGCTCA GGAATGCCGA TGTGGAGGCC CGTATCGGGG TGGCCGGGTC TAAGAAGAAG 
AGCACCCGTG CCAGACTGGT GTTTCGAGTC AACATCCCAC GCCCCGATGG CTCTGCGCTC 
ACGTTACAGA CCTCGTCGTC TCCCATACTG TGCACCCAGC CTGCAGGAGC ACCTGAGATT 
CTCAAGAAGA GTTTACACAG CTGTTCGGTT AAAGGGGGGA ATGAAGTCTT TATCATTGGA 
AAAAACTTTC TGAAAGGAAC CAAGGTCATA TTTCAGGAGA ACGCTTCAGA TGATAAGTCC 
TGGAAGGCAG AGGCTGACAT TGACATGGAG TTTTTCCATC AGAATCATCT TATTGTCAAA 
GTCCCACCAT ATCAAAATCC AGCTATCACA TCTCCTATAT CTGTTGGTAT CTATGTTGTC 
ACGAATGCTG GGCGTTCACA TGATGTTCAA CAATTTACAT ACACTCCAGA GCCAGTGGTT 
GATTCTTCTG TAAAAAAGGA GGACTTGCCT CCAGTGACAC CTTGTTGTTT TGAACAAATT 
AAAGTAATTG ATACTGCCGT GATGACACCA GGCTTACCTC TTGTGAAACG TGAAGAGGCC 
ACTCCGATGG AGGTCTCCGG TAACCCTTCG GACTTCAAGC AGACCTCTGA AGTCATCAAT 
TCAGCCCAGC AAGCTTTAGA CATGAGTACT GAGATTTCCA CCAACAACTG CAAATTTTCC 
AGTCCGTTGG CACACCCAAC AAGAGAGCCA GATCAATCTC AGAATTCAGT CTTTTCCAAC 
AAAGAACCTT TCAACACCAT CCAGGAACAG AATCTTGCAT CCAGTGGCTC TTTCCATGTG 
CCTAATAAAT CTCTTCTCCA GCAAGGAGTT CAGCCATTCC TCCTGGAACA CAGAGACCGT 
CTTGGACAGG AGATGCCAGG CAGCAGTGGC AGTTCAGTAG TGGCTCTTTC CCAATTAGAT 
GACAATCCGC AGCAACAGCT CTCTCTTCTA CCTCCAGATG AGGTGGCCCA ACTGGAGCAG 
GCCGTGCGGC AGCTGCAAGC GAAAGGATAC TGCGGCATAC AACTCCAGAG TGAGAATTCA 
CTGTCAAAGC AAAGACAACA TCAGCTTCAA CAGCAGCAAC AGCAAATCCA ACAACAGCAG 
CATCAAATCC ACCAACAACA GCAGCAAATT CAGCAACAGC AGCAGCACAT CCAGCATCAA 
GAGCAGCAAA TCCAGCTGCA GCAGCAGCAC CACCACCAGC AGCAAGCTCT GGAGAACCTT 
CAACATCAGC TTTTTCAGCC CCAGGTTCAA ATTCACTGTA GTTCAGAGCA GCAGAGCTCT 
TCCCAGGGTA TGGTGCAGAA TGGAAGTTCC CTTTTCCAGT CCACCCCATC CCAACAACAA 
CAGCAGCAGC AAGCAACTTT GTTCCAACAA GGAGCTGGCT TTCTTCAGCA GCAACCCAGT 
CATTCCTCTC CTTCCATGTT CCACAACACT ACAGCAATGA CCGAAGCTCA AAGCCCTCAG 
GAGTCTCTTT TCCACACACA AAAGACCTCT CCCAACCAGG ATCAGGTCCA AGCTTCCATT 
TTCCAAAGCA ATCTTTCGGT TCTCAGTGGG TCCAACATGT CTTCAGAAGC TCAGCCCTCT 
GCAGCTAGTA TGTTTCTTCC CCAGAACACA GTACCAGCTC AACTTTCCGT TAACGCCAAC 
CAGCCACAGC AACTGGCGTT CCTCACTTCC ATGCAGACAT CTCTTGAGGC GCCATCTGTA 
TTTCAGACTC CAACCCAACT GTCCCCCATT CAGCAAGGCA CTCCGATGGA TCAGCAGCAG 
TCCCCACAGC ATGCCCAACC GGGTTCCATG TTCCAGAGCA TCTCCCAATC ACCAAGCAAA 
CTCACCCCTG GTCAACAGCA GCAGGCTGGT CTACTCCTCG GATCCTTAAA CTCTCCAATC 
ACACAAGAGC AATCCTCCAA TCTGTTATTC GGTGGCCAAG TACAGATAGG ATCAATGAAC 
AATAGCAGCC TAGCTTCGTC AGAGCAACAG AACACTCCCC TTCCCTTCGC TCAGGCTGGT 
CTGGTCACCG TGAGGCAGCA GGAGGCATCT GAACCAATGA CATTTCAGGA TCAGACAGCA 
ATGGTGGTCA ACCTGTCATC AAATAAGCCC ATCTTCCAAG ACCAGCAGCC AATGCAGTTG 
GCACCAAGTT CTAATCCAGG CTCTTCACAG TCTGTAAATC TTTTCATGGC ACAGTCCAAC 
ATGCAAGGGG GGATGACTAC ACAAGAAGCT CTTTTTGCAC CACAAGGAGA TGTAGGTGGC 
ATTCAGACAA GCACTTCCTC TCCAGTGCAA CAGCCTGGTC AACTCTTTCA GACCACAATG 
AGTGGGAGTC TCAACCAGCC CAACCAGGAT CAGCAGACAG ACCTATTCCT CTTCGAGATC 
CAGAATGAGT GCCGTCAACT CATGAACAGC CAAGGATCTA CACTGTCTAA TCAAATAATT 
ACCATCAATC AGCCTGGACA GAGTCAGCAA GAGAGTGATG GGCAAATCCA GTCTTTACTT 
GGGCAGGCTA TGCCAGACCC TGGAGCTGTG CAGAGTACCT TGACAACCTC TCAGAACATG 
GAGAAAATTG ATGACCTTCT GGTGAGCCTT CAGGAGCAAG GCAATAACAT CTCCCATTCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_021478040.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333715.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X6, mRNA.

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CloneID	ODa143565
Clone ID Related Accession (Same CDS sequence)	XM_021478040.1
Accession Version	XM_021478040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTG GAAGTCCTCG CAGTGCTTTT CCCACCTCAG CGTCAGATCG AAGCACCGTA 
CACGACAGCG GCTCATCAGG AGATGTCAGC AGTCGAAGGA AATCGGAGGC TGTGGAAAAC 
AAACCCTCTG GTCGAGATAA AGGTGTCCAG CAGACCCAGA TGACCCCCTC CAAACGGCCT 
GTCCTCAACA TCTCGCCTCC ACCCGAAGAC CTGTTCGATG ACAGCCACAT GTCCTGCCAG 
GATGATCTGC TTCTCGACTC GGAGCAAAGC AGCAGCATCT GGATTGAAAA CTCAGTGTCT 
AACTTCAGCA TTGTGAGCTC TAGTTCCTAC AACGACAACA CTGAAGTGCC CCGCAAGGCC 
CGCAAATGTA CCCCGCGACA GAAGCCTGGG CCGAAGCCGG TTTCTGCGGA AGAGGCCAGC 
ATGGATGTCT TTGATGCAGA CAGTGCCAAA GGCCCGCACT TTGTGCTCTC ACAACTCGGC 
CCAGACAGCA AGACCTGTTC CAAAGCAAGC TCTGATGGAC CACAAGTGTC TGCTCTCAAA 
GGTGGGGTAC TGTCTTGTCA GTATCCTCAG AAAGGTGAAG GGAAAGAACT GAAGATCGTG 
GTGCAGCCTG AGACCCAGCA CAGAGCGCGT TACCTGACTG AAGGCAGCCG AGGGTCAGTC 
AAAGACCAAA CACAGCAGGG CTTCCCTACT ATCAAGCTTG AGGGTGTGAA GGAGTCAGTG 
GTCATGCAGA TCTTTGTGGG CAATGATGCC GGACGTGTGA AGCCTCACGG GTTCTACCAG 
GCTTGCAGAG TCACCGGCCG AAACACAACC GCCTGCAGGG AAGTGGACAT CGAAGGCACC 
ACAGTCATCG AAGTGCCAAT GGATCCCAGC AGCAACATGA CTTTACCGTG TCTCTGCAGT 
GTGGACTGTG TGGGGATCCT GAAGCTCAGG AATGCCGATG TGGAGGCCCG TATCGGGGTG 
GCCGGGTCTA AGAAGAAGAG CACCCGTGCC AGACTGGTGT TTCGAGTCAA CATCCCACGC 
CCCGATGGCT CTGCGCTCAC GTTACAGACC TCGTCGTCTC CCATACTGTG CACCCAGCCT 
GCAGGAGCAC CTGAGATTCT CAAGAAGAGT TTACACAGCT GTTCGGTTAA AGGGGGGAAT 
GAAGTCTTTA TCATTGGAAA AAACTTTCTG AAAGGAACCA AGGTCATATT TCAGGAGAAC 
GCTTCAGCAG ATGATAAGTC CTGGAAGGCA GAGGCTGACA TTGACATGGA GTTTTTCCAT 
CAGAATCATC TTATTGTCAA AGTCCCACCA TATCAAAATC CAGCTATCAC ATCTCCTATA 
TCTGTTGGTA TCTATGTTGT CACGAATGCT GGGCGTTCAC ATGATGTTCA ACAATTTACA 
TACACTCCAG AGCCAGTGGT TGATTCTTCT GTAAAAAAGG AGGACTTGCC TCCAGTGACA 
CCTTGTTGTT TTGAACAAAT TAAAGTAATT GATACTGCCG TGATGACACC AGGCTTACCT 
CTTGTGAAAC GTGAAGAGGC CACTCCGATG GAGGTCTCCG GTAACCCTTC GGACTTCAAG 
CAGACCTCTG AAGTCATCAA TTCAGCCCAG CAAGCTTTAG ACATGAGTAC TGAGATTTCC 
ACCAACAACT GCAAATTTTC CAGTCCGTTG GCACACCCAA CAAGAGAGCC AGATCAATCT 
CAGAATTCAG TCTTTTCCAA CAAAGAACCT TTCAACACCA TCCAGGAACA GAATCTTGCA 
TCCAGTGGCT CTTTCCATGT GCCTAATAAA TCTCTTCTCC AGCAAGGAGT TCAGCCATTC 
CTCCTGGAAC ACAGAGACCG TCTTGGACAG GAGATGCCAG GCAGCAGTGG CAGTTCAGTA 
GTGGCTCTTT CCCAATTAGA TGACAATCCG CAGCAACAGC TCTCTCTTCT ACCTCCAGAT 
GAGGTGGCCC AACTGGAGCA GGCCGTGCGG CAGCTGCAAG CGAAAGGATA CTGCGGCATA 
CAACTCCAGA GTGAGAATTC ACTGTCAAAG CAAAGACAAC ATCAGCTTCA ACAGCAGCAA 
CAGCAAATCC AACAACAGCA GCATCAAATC CACCAACAAC AGCAGCAAAT TCAGCAACAG 
CAGCAGCACA TCCAGCATCA AGAGCAGCAA ATCCAGCTGC AGCAGCAGCA CCACCACCAG 
CAGCAAGCTC TGGAGAACCT TCAACATCAG CTTTTTCAGC CCCAGGTTCA AATTCACTGT 
AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG 
TCCACCCCAT CCCAACAACA ACAGCAGCAG CAAGCAACTT TGTTCCAACA AGGAGCTGGC 
TTTCTTCAGC AGCAACCCAG TCATTCCTCT CCTTCCATGT TCCACAACAC TACAGCAATG 
ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT TTCCACACAC AAAAGACCTC TCCCAACCAG 
GATCAGGTCC AAGCTTCCAT TTTCCAAAGC AATCTTTCGG TTCTCAGTGG GTCCAACATG 
TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT ATGTTTCTTC CCCAGAACAC AGTACCAGCT 
CAACTTTCCG TTAACGCCAA CCAGCCACAG CAACTGGCGT TCCTCACTTC CATGCAGACA 
TCTCTTGAGG CGCCATCTGT ATTTCAGACT CCAACCCAAC TGTCCCCCAT TCAGCAAGGC 
ACTCCGATGG ATCAGCAGCA GTCCCCACAG CATGCCCAAC CGGGTTCCAT GTTCCAGAGC 
ATCTCCCAAT CACCAAGCAA ACTCACCCCT GGTCAACAGC AGCAGGCTGG TCTACTCCTC 
GGATCCTTAA ACTCTCCAAT CACACAAGAG CAATCCTCCA ATCTGTTATT CGGTGGCCAA 
GTACAGATAG GATCAATGAA CAATAGCAGC CTAGCTTCGT CAGAGCAACA GAACACTCCC 
CTTCCCTTCG CTCAGGCTGG TCTGGTCACC GTGAGGCAGC AGGAGGCATC TGAACCAATG 
ACATTTCAGG ATCAGACAGC AATGGTGGTC AACCTGTCAT CAAATAAGCC CATCTTCCAA 
GACCAGCAGC CAATGCAGTT GGCACCAAGT TCTAATCCAG GCTCTTCACA GTCTGTAAAT 
CTTTTCATGG CACAGTCCAA CATGCAAGGG GGGATGACTA CACAAGAAGC TCTTTTTGCA 
CCACAAGGAG ATGTAGGTGG CATTCAGACA AGCACTTCCT CTCCAGTGCA ACAGCCTGGT 
CAACTCTTTC AGACCACAAT GAGTGGGAGT CTCAACCAGC CCAACCAGGA TCAGCAGACA 
GACCTATTCC TCTTCGAGAT CCAGAATGAG TGCCGTCAAC TCATGAACAG CCAAGGATCT 
ACACTGTCTA ATCAAATAAT TACCATCAAT CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT 
GGGCAAATCC AGTCTTTACT TGGGCAGGCT ATGCCAGACC CTGGAGCTGT GCAGAGTACC 
TTGACAACCT CTCAGAACAT GGAGAAAATT GATGACCTTC TGGTGAGCCT TCAGGAGCAA 
GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333715.1
CDS	176..3742
Translation	MSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPCLCSVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_021478040.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478040.1

ORF Insert Sequence:

ATGAGCTCTG GAAGTCCTCG CAGTGCTTTT CCCACCTCAG CGTCAGATCG AAGCACCGTA 
CACGACAGCG GCTCATCAGG AGATGTCAGC AGTCGAAGGA AATCGGAGGC TGTGGAAAAC 
AAACCCTCTG GTCGAGATAA AGGTGTCCAG CAGACCCAGA TGACCCCCTC CAAACGGCCT 
GTCCTCAACA TCTCGCCTCC ACCCGAAGAC CTGTTCGATG ACAGCCACAT GTCCTGCCAG 
GATGATCTGC TTCTCGACTC GGAGCAAAGC AGCAGCATCT GGATTGAAAA CTCAGTGTCT 
AACTTCAGCA TTGTGAGCTC TAGTTCCTAC AACGACAACA CTGAAGTGCC CCGCAAGGCC 
CGCAAATGTA CCCCGCGACA GAAGCCTGGG CCGAAGCCGG TTTCTGCGGA AGAGGCCAGC 
ATGGATGTCT TTGATGCAGA CAGTGCCAAA GGCCCGCACT TTGTGCTCTC ACAACTCGGC 
CCAGACAGCA AGACCTGTTC CAAAGCAAGC TCTGATGGAC CACAAGTGTC TGCTCTCAAA 
GGTGGGGTAC TGTCTTGTCA GTATCCTCAG AAAGGTGAAG GGAAAGAACT GAAGATCGTG 
GTGCAGCCTG AGACCCAGCA CAGAGCGCGT TACCTGACTG AAGGCAGCCG AGGGTCAGTC 
AAAGACCAAA CACAGCAGGG CTTCCCTACT ATCAAGCTTG AGGGTGTGAA GGAGTCAGTG 
GTCATGCAGA TCTTTGTGGG CAATGATGCC GGACGTGTGA AGCCTCACGG GTTCTACCAG 
GCTTGCAGAG TCACCGGCCG AAACACAACC GCCTGCAGGG AAGTGGACAT CGAAGGCACC 
ACAGTCATCG AAGTGCCAAT GGATCCCAGC AGCAACATGA CTTTACCGTG TCTCTGCAGT 
GTGGACTGTG TGGGGATCCT GAAGCTCAGG AATGCCGATG TGGAGGCCCG TATCGGGGTG 
GCCGGGTCTA AGAAGAAGAG CACCCGTGCC AGACTGGTGT TTCGAGTCAA CATCCCACGC 
CCCGATGGCT CTGCGCTCAC GTTACAGACC TCGTCGTCTC CCATACTGTG CACCCAGCCT 
GCAGGAGCAC CTGAGATTCT CAAGAAGAGT TTACACAGCT GTTCGGTTAA AGGGGGGAAT 
GAAGTCTTTA TCATTGGAAA AAACTTTCTG AAAGGAACCA AGGTCATATT TCAGGAGAAC 
GCTTCAGCAG ATGATAAGTC CTGGAAGGCA GAGGCTGACA TTGACATGGA GTTTTTCCAT 
CAGAATCATC TTATTGTCAA AGTCCCACCA TATCAAAATC CAGCTATCAC ATCTCCTATA 
TCTGTTGGTA TCTATGTTGT CACGAATGCT GGGCGTTCAC ATGATGTTCA ACAATTTACA 
TACACTCCAG AGCCAGTGGT TGATTCTTCT GTAAAAAAGG AGGACTTGCC TCCAGTGACA 
CCTTGTTGTT TTGAACAAAT TAAAGTAATT GATACTGCCG TGATGACACC AGGCTTACCT 
CTTGTGAAAC GTGAAGAGGC CACTCCGATG GAGGTCTCCG GTAACCCTTC GGACTTCAAG 
CAGACCTCTG AAGTCATCAA TTCAGCCCAG CAAGCTTTAG ACATGAGTAC TGAGATTTCC 
ACCAACAACT GCAAATTTTC CAGTCCGTTG GCACACCCAA CAAGAGAGCC AGATCAATCT 
CAGAATTCAG TCTTTTCCAA CAAAGAACCT TTCAACACCA TCCAGGAACA GAATCTTGCA 
TCCAGTGGCT CTTTCCATGT GCCTAATAAA TCTCTTCTCC AGCAAGGAGT TCAGCCATTC 
CTCCTGGAAC ACAGAGACCG TCTTGGACAG GAGATGCCAG GCAGCAGTGG CAGTTCAGTA 
GTGGCTCTTT CCCAATTAGA TGACAATCCG CAGCAACAGC TCTCTCTTCT ACCTCCAGAT 
GAGGTGGCCC AACTGGAGCA GGCCGTGCGG CAGCTGCAAG CGAAAGGATA CTGCGGCATA 
CAACTCCAGA GTGAGAATTC ACTGTCAAAG CAAAGACAAC ATCAGCTTCA ACAGCAGCAA 
CAGCAAATCC AACAACAGCA GCATCAAATC CACCAACAAC AGCAGCAAAT TCAGCAACAG 
CAGCAGCACA TCCAGCATCA AGAGCAGCAA ATCCAGCTGC AGCAGCAGCA CCACCACCAG 
CAGCAAGCTC TGGAGAACCT TCAACATCAG CTTTTTCAGC CCCAGGTTCA AATTCACTGT 
AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG 
TCCACCCCAT CCCAACAACA ACAGCAGCAG CAAGCAACTT TGTTCCAACA AGGAGCTGGC 
TTTCTTCAGC AGCAACCCAG TCATTCCTCT CCTTCCATGT TCCACAACAC TACAGCAATG 
ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT TTCCACACAC AAAAGACCTC TCCCAACCAG 
GATCAGGTCC AAGCTTCCAT TTTCCAAAGC AATCTTTCGG TTCTCAGTGG GTCCAACATG 
TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT ATGTTTCTTC CCCAGAACAC AGTACCAGCT 
CAACTTTCCG TTAACGCCAA CCAGCCACAG CAACTGGCGT TCCTCACTTC CATGCAGACA 
TCTCTTGAGG CGCCATCTGT ATTTCAGACT CCAACCCAAC TGTCCCCCAT TCAGCAAGGC 
ACTCCGATGG ATCAGCAGCA GTCCCCACAG CATGCCCAAC CGGGTTCCAT GTTCCAGAGC 
ATCTCCCAAT CACCAAGCAA ACTCACCCCT GGTCAACAGC AGCAGGCTGG TCTACTCCTC 
GGATCCTTAA ACTCTCCAAT CACACAAGAG CAATCCTCCA ATCTGTTATT CGGTGGCCAA 
GTACAGATAG GATCAATGAA CAATAGCAGC CTAGCTTCGT CAGAGCAACA GAACACTCCC 
CTTCCCTTCG CTCAGGCTGG TCTGGTCACC GTGAGGCAGC AGGAGGCATC TGAACCAATG 
ACATTTCAGG ATCAGACAGC AATGGTGGTC AACCTGTCAT CAAATAAGCC CATCTTCCAA 
GACCAGCAGC CAATGCAGTT GGCACCAAGT TCTAATCCAG GCTCTTCACA GTCTGTAAAT 
CTTTTCATGG CACAGTCCAA CATGCAAGGG GGGATGACTA CACAAGAAGC TCTTTTTGCA 
CCACAAGGAG ATGTAGGTGG CATTCAGACA AGCACTTCCT CTCCAGTGCA ACAGCCTGGT 
CAACTCTTTC AGACCACAAT GAGTGGGAGT CTCAACCAGC CCAACCAGGA TCAGCAGACA 
GACCTATTCC TCTTCGAGAT CCAGAATGAG TGCCGTCAAC TCATGAACAG CCAAGGATCT 
ACACTGTCTA ATCAAATAAT TACCATCAAT CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT 
GGGCAAATCC AGTCTTTACT TGGGCAGGCT ATGCCAGACC CTGGAGCTGT GCAGAGTACC 
TTGACAACCT CTCAGAACAT GGAGAAAATT GATGACCTTC TGGTGAGCCT TCAGGAGCAA 
GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303605.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301880.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42239
Clone ID Related Accession (Same CDS sequence)	XM_009303605.3 , XM_009303605.2
Accession Version	XM_009303605.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303605.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG CAGACCTCTG AAGTCATCAA TTCAGCCCAG 
CAAGCTTTAG ACATGAGTAC TGAGATTTCC ACCAACAACT GCAAATTTTC CAGTCCGTTG 
GCACACCCAA CAAGAGAGCC AGATCAATCT CAGAATTCAG TCTTTTCCAA CAAAGAACCT 
TTCAACACCA TCCAGGAACA GAATCTTGCA TCCAGTGGCT CTTTCCATGT GCCTAATAAA 
TCTCTTCTCC AGCAAGGAGT TCAGCCATTC CTCCTGGAAC ACAGAGACCG TCTTGGACAG 
GAGATGCCAG GCAGCAGTGG CAGTTCAGTA GTGGCTCTTT CCCAATTAGA TGACAATCCG 
CAGCAACAGC TCTCTCTTCT ACCTCCAGAT GAGGTGGCCC AACTGGAGCA GGCCGTGCGG 
CAGCTGCAAG CGAAAGGATA CTGCGGCATA CAACTCCAGA GTGAGAATTC ACTGTCAAAG 
CAAAGACAAC ATCAGCTTCA ACAGCAGCAA CAGCAAATCC AACAACAGCA GCATCAAATC 
CACCAACAAC AGCAGCAAAT TCAGCAACAG CAGCAGCACA TCCAGCATCA AGAGCAGCAA 
ATCCAGCTGC AGCAGCAGCA CCACCACCAG CAGCAAGCTC TGGAGAACCT TCAACATCAG 
CTTTTTCAGC CCCAGGTTCA AATTCACTGT AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT 
ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG TCCACCCCAT CCCAACAACA ACAGCAGCAG 
CAAGCAACTT TGTTCCAACA AGGAGCTGGC TTTCTTCAGC AGCAACCCAG TCATTCCTCT 
CCTTCCATGT TCCACAACAC TACAGCAATG ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT 
TTCCACACAC AAAAGACCTC TCCCAACCAG GATCAGGTCC AAGCTTCCAT TTTCCAAAGC 
AATCTTTCGG TTCTCAGTGG GTCCAACATG TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT 
ATGTTTCTTC CCCAGAACAC AGTACCAGCT CAACTTTCCG TTAACGCCAA CCAGCCACAG 
CAACTGGCGT TCCTCACTTC CATGCAGACA TCTCTTGAGG CGCCATCTGT ATTTCAGACT 
CCAACCCAAC TGTCCCCCAT TCAGCAAGGC ACTCCGATGG ATCAGCAGCA GTCCCCACAG 
CATGCCCAAC CGGGTTCCAT GTTCCAGAGC ATCTCCCAAT CACCAAGCAA ACTCACCCCT 
GGTCAACAGC AGCAGGCTGG TCTACTCCTC GGATCCTTAA ACTCTCCAAT CACACAAGAG 
CAATCCTCCA ATCTGTTATT CGGTGGCCAA GTACAGATAG GATCAATGAA CAATAGCAGC 
CTAGCTTCGT CAGAGCAACA GAACACTCCC CTTCCCTTCG CTCAGGCTGG TCTGGTCACC 
GTGAGGCAGC AGGAGGCATC TGAACCAATG ACATTTCAGG ATCAGACAGC AATGGTGGTC 
AACCTGTCAT CAAATAAGCC CATCTTCCAA GACCAGCAGC CAATGCAGTT GGCACCAAGT 
TCTAATCCAG GCTCTTCACA GTCTGTAAAT CTTTTCATGG CACAGTCCAA CATGCAAGGG 
GGGATGACTA CACAAGAAGC TCTTTTTGCA CCACAAGGAG ATGTAGGTGG CATTCAGACA 
AGCACTTCCT CTCCAGTGCA ACAGCCTGGT CAACTCTTTC AGACCACAAT GAGTGGGAGT 
CTCAACCAGC CCAACCAGGA TCAGCAGACA GACCTATTCC TCTTCGAGAT CCAGAATGAG 
TGCCGTCAAC TCATGAACAG CCAAGGATCT ACACTGTCTA ATCAAATAAT TACCATCAAT 
CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT GGGCAAATCC AGTCTTTACT TGGGCAGGCT 
ATGCCAGACC CTGGAGCTGT GCAGAGTACC TTGACAACCT CTCAGAACAT GGAGAAAATT 
GATGACCTTC TGGTGAGCCT TCAGGAGCAA GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301880.1
CDS	261..4037
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303605.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303605.3

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG CAGACCTCTG AAGTCATCAA TTCAGCCCAG 
CAAGCTTTAG ACATGAGTAC TGAGATTTCC ACCAACAACT GCAAATTTTC CAGTCCGTTG 
GCACACCCAA CAAGAGAGCC AGATCAATCT CAGAATTCAG TCTTTTCCAA CAAAGAACCT 
TTCAACACCA TCCAGGAACA GAATCTTGCA TCCAGTGGCT CTTTCCATGT GCCTAATAAA 
TCTCTTCTCC AGCAAGGAGT TCAGCCATTC CTCCTGGAAC ACAGAGACCG TCTTGGACAG 
GAGATGCCAG GCAGCAGTGG CAGTTCAGTA GTGGCTCTTT CCCAATTAGA TGACAATCCG 
CAGCAACAGC TCTCTCTTCT ACCTCCAGAT GAGGTGGCCC AACTGGAGCA GGCCGTGCGG 
CAGCTGCAAG CGAAAGGATA CTGCGGCATA CAACTCCAGA GTGAGAATTC ACTGTCAAAG 
CAAAGACAAC ATCAGCTTCA ACAGCAGCAA CAGCAAATCC AACAACAGCA GCATCAAATC 
CACCAACAAC AGCAGCAAAT TCAGCAACAG CAGCAGCACA TCCAGCATCA AGAGCAGCAA 
ATCCAGCTGC AGCAGCAGCA CCACCACCAG CAGCAAGCTC TGGAGAACCT TCAACATCAG 
CTTTTTCAGC CCCAGGTTCA AATTCACTGT AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT 
ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG TCCACCCCAT CCCAACAACA ACAGCAGCAG 
CAAGCAACTT TGTTCCAACA AGGAGCTGGC TTTCTTCAGC AGCAACCCAG TCATTCCTCT 
CCTTCCATGT TCCACAACAC TACAGCAATG ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT 
TTCCACACAC AAAAGACCTC TCCCAACCAG GATCAGGTCC AAGCTTCCAT TTTCCAAAGC 
AATCTTTCGG TTCTCAGTGG GTCCAACATG TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT 
ATGTTTCTTC CCCAGAACAC AGTACCAGCT CAACTTTCCG TTAACGCCAA CCAGCCACAG 
CAACTGGCGT TCCTCACTTC CATGCAGACA TCTCTTGAGG CGCCATCTGT ATTTCAGACT 
CCAACCCAAC TGTCCCCCAT TCAGCAAGGC ACTCCGATGG ATCAGCAGCA GTCCCCACAG 
CATGCCCAAC CGGGTTCCAT GTTCCAGAGC ATCTCCCAAT CACCAAGCAA ACTCACCCCT 
GGTCAACAGC AGCAGGCTGG TCTACTCCTC GGATCCTTAA ACTCTCCAAT CACACAAGAG 
CAATCCTCCA ATCTGTTATT CGGTGGCCAA GTACAGATAG GATCAATGAA CAATAGCAGC 
CTAGCTTCGT CAGAGCAACA GAACACTCCC CTTCCCTTCG CTCAGGCTGG TCTGGTCACC 
GTGAGGCAGC AGGAGGCATC TGAACCAATG ACATTTCAGG ATCAGACAGC AATGGTGGTC 
AACCTGTCAT CAAATAAGCC CATCTTCCAA GACCAGCAGC CAATGCAGTT GGCACCAAGT 
TCTAATCCAG GCTCTTCACA GTCTGTAAAT CTTTTCATGG CACAGTCCAA CATGCAAGGG 
GGGATGACTA CACAAGAAGC TCTTTTTGCA CCACAAGGAG ATGTAGGTGG CATTCAGACA 
AGCACTTCCT CTCCAGTGCA ACAGCCTGGT CAACTCTTTC AGACCACAAT GAGTGGGAGT 
CTCAACCAGC CCAACCAGGA TCAGCAGACA GACCTATTCC TCTTCGAGAT CCAGAATGAG 
TGCCGTCAAC TCATGAACAG CCAAGGATCT ACACTGTCTA ATCAAATAAT TACCATCAAT 
CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT GGGCAAATCC AGTCTTTACT TGGGCAGGCT 
ATGCCAGACC CTGGAGCTGT GCAGAGTACC TTGACAACCT CTCAGAACAT GGAGAAAATT 
GATGACCTTC TGGTGAGCCT TCAGGAGCAA GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303605.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301880.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.

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CloneID	ODa42239
Clone ID Related Accession (Same CDS sequence)	XM_009303605.3 , XM_009303605.2
Accession Version	XM_009303605.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG CAGACCTCTG AAGTCATCAA TTCAGCCCAG 
CAAGCTTTAG ACATGAGTAC TGAGATTTCC ACCAACAACT GCAAATTTTC CAGTCCGTTG 
GCACACCCAA CAAGAGAGCC AGATCAATCT CAGAATTCAG TCTTTTCCAA CAAAGAACCT 
TTCAACACCA TCCAGGAACA GAATCTTGCA TCCAGTGGCT CTTTCCATGT GCCTAATAAA 
TCTCTTCTCC AGCAAGGAGT TCAGCCATTC CTCCTGGAAC ACAGAGACCG TCTTGGACAG 
GAGATGCCAG GCAGCAGTGG CAGTTCAGTA GTGGCTCTTT CCCAATTAGA TGACAATCCG 
CAGCAACAGC TCTCTCTTCT ACCTCCAGAT GAGGTGGCCC AACTGGAGCA GGCCGTGCGG 
CAGCTGCAAG CGAAAGGATA CTGCGGCATA CAACTCCAGA GTGAGAATTC ACTGTCAAAG 
CAAAGACAAC ATCAGCTTCA ACAGCAGCAA CAGCAAATCC AACAACAGCA GCATCAAATC 
CACCAACAAC AGCAGCAAAT TCAGCAACAG CAGCAGCACA TCCAGCATCA AGAGCAGCAA 
ATCCAGCTGC AGCAGCAGCA CCACCACCAG CAGCAAGCTC TGGAGAACCT TCAACATCAG 
CTTTTTCAGC CCCAGGTTCA AATTCACTGT AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT 
ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG TCCACCCCAT CCCAACAACA ACAGCAGCAG 
CAAGCAACTT TGTTCCAACA AGGAGCTGGC TTTCTTCAGC AGCAACCCAG TCATTCCTCT 
CCTTCCATGT TCCACAACAC TACAGCAATG ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT 
TTCCACACAC AAAAGACCTC TCCCAACCAG GATCAGGTCC AAGCTTCCAT TTTCCAAAGC 
AATCTTTCGG TTCTCAGTGG GTCCAACATG TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT 
ATGTTTCTTC CCCAGAACAC AGTACCAGCT CAACTTTCCG TTAACGCCAA CCAGCCACAG 
CAACTGGCGT TCCTCACTTC CATGCAGACA TCTCTTGAGG CGCCATCTGT ATTTCAGACT 
CCAACCCAAC TGTCCCCCAT TCAGCAAGGC ACTCCGATGG ATCAGCAGCA GTCCCCACAG 
CATGCCCAAC CGGGTTCCAT GTTCCAGAGC ATCTCCCAAT CACCAAGCAA ACTCACCCCT 
GGTCAACAGC AGCAGGCTGG TCTACTCCTC GGATCCTTAA ACTCTCCAAT CACACAAGAG 
CAATCCTCCA ATCTGTTATT CGGTGGCCAA GTACAGATAG GATCAATGAA CAATAGCAGC 
CTAGCTTCGT CAGAGCAACA GAACACTCCC CTTCCCTTCG CTCAGGCTGG TCTGGTCACC 
GTGAGGCAGC AGGAGGCATC TGAACCAATG ACATTTCAGG ATCAGACAGC AATGGTGGTC 
AACCTGTCAT CAAATAAGCC CATCTTCCAA GACCAGCAGC CAATGCAGTT GGCACCAAGT 
TCTAATCCAG GCTCTTCACA GTCTGTAAAT CTTTTCATGG CACAGTCCAA CATGCAAGGG 
GGGATGACTA CACAAGAAGC TCTTTTTGCA CCACAAGGAG ATGTAGGTGG CATTCAGACA 
AGCACTTCCT CTCCAGTGCA ACAGCCTGGT CAACTCTTTC AGACCACAAT GAGTGGGAGT 
CTCAACCAGC CCAACCAGGA TCAGCAGACA GACCTATTCC TCTTCGAGAT CCAGAATGAG 
TGCCGTCAAC TCATGAACAG CCAAGGATCT ACACTGTCTA ATCAAATAAT TACCATCAAT 
CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT GGGCAAATCC AGTCTTTACT TGGGCAGGCT 
ATGCCAGACC CTGGAGCTGT GCAGAGTACC TTGACAACCT CTCAGAACAT GGAGAAAATT 
GATGACCTTC TGGTGAGCCT TCAGGAGCAA GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301880.1
CDS	245..4021
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303605.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303605.2

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG CAGACCTCTG AAGTCATCAA TTCAGCCCAG 
CAAGCTTTAG ACATGAGTAC TGAGATTTCC ACCAACAACT GCAAATTTTC CAGTCCGTTG 
GCACACCCAA CAAGAGAGCC AGATCAATCT CAGAATTCAG TCTTTTCCAA CAAAGAACCT 
TTCAACACCA TCCAGGAACA GAATCTTGCA TCCAGTGGCT CTTTCCATGT GCCTAATAAA 
TCTCTTCTCC AGCAAGGAGT TCAGCCATTC CTCCTGGAAC ACAGAGACCG TCTTGGACAG 
GAGATGCCAG GCAGCAGTGG CAGTTCAGTA GTGGCTCTTT CCCAATTAGA TGACAATCCG 
CAGCAACAGC TCTCTCTTCT ACCTCCAGAT GAGGTGGCCC AACTGGAGCA GGCCGTGCGG 
CAGCTGCAAG CGAAAGGATA CTGCGGCATA CAACTCCAGA GTGAGAATTC ACTGTCAAAG 
CAAAGACAAC ATCAGCTTCA ACAGCAGCAA CAGCAAATCC AACAACAGCA GCATCAAATC 
CACCAACAAC AGCAGCAAAT TCAGCAACAG CAGCAGCACA TCCAGCATCA AGAGCAGCAA 
ATCCAGCTGC AGCAGCAGCA CCACCACCAG CAGCAAGCTC TGGAGAACCT TCAACATCAG 
CTTTTTCAGC CCCAGGTTCA AATTCACTGT AGTTCAGAGC AGCAGAGCTC TTCCCAGGGT 
ATGGTGCAGA ATGGAAGTTC CCTTTTCCAG TCCACCCCAT CCCAACAACA ACAGCAGCAG 
CAAGCAACTT TGTTCCAACA AGGAGCTGGC TTTCTTCAGC AGCAACCCAG TCATTCCTCT 
CCTTCCATGT TCCACAACAC TACAGCAATG ACCGAAGCTC AAAGCCCTCA GGAGTCTCTT 
TTCCACACAC AAAAGACCTC TCCCAACCAG GATCAGGTCC AAGCTTCCAT TTTCCAAAGC 
AATCTTTCGG TTCTCAGTGG GTCCAACATG TCTTCAGAAG CTCAGCCCTC TGCAGCTAGT 
ATGTTTCTTC CCCAGAACAC AGTACCAGCT CAACTTTCCG TTAACGCCAA CCAGCCACAG 
CAACTGGCGT TCCTCACTTC CATGCAGACA TCTCTTGAGG CGCCATCTGT ATTTCAGACT 
CCAACCCAAC TGTCCCCCAT TCAGCAAGGC ACTCCGATGG ATCAGCAGCA GTCCCCACAG 
CATGCCCAAC CGGGTTCCAT GTTCCAGAGC ATCTCCCAAT CACCAAGCAA ACTCACCCCT 
GGTCAACAGC AGCAGGCTGG TCTACTCCTC GGATCCTTAA ACTCTCCAAT CACACAAGAG 
CAATCCTCCA ATCTGTTATT CGGTGGCCAA GTACAGATAG GATCAATGAA CAATAGCAGC 
CTAGCTTCGT CAGAGCAACA GAACACTCCC CTTCCCTTCG CTCAGGCTGG TCTGGTCACC 
GTGAGGCAGC AGGAGGCATC TGAACCAATG ACATTTCAGG ATCAGACAGC AATGGTGGTC 
AACCTGTCAT CAAATAAGCC CATCTTCCAA GACCAGCAGC CAATGCAGTT GGCACCAAGT 
TCTAATCCAG GCTCTTCACA GTCTGTAAAT CTTTTCATGG CACAGTCCAA CATGCAAGGG 
GGGATGACTA CACAAGAAGC TCTTTTTGCA CCACAAGGAG ATGTAGGTGG CATTCAGACA 
AGCACTTCCT CTCCAGTGCA ACAGCCTGGT CAACTCTTTC AGACCACAAT GAGTGGGAGT 
CTCAACCAGC CCAACCAGGA TCAGCAGACA GACCTATTCC TCTTCGAGAT CCAGAATGAG 
TGCCGTCAAC TCATGAACAG CCAAGGATCT ACACTGTCTA ATCAAATAAT TACCATCAAT 
CAGCCTGGAC AGAGTCAGCA AGAGAGTGAT GGGCAAATCC AGTCTTTACT TGGGCAGGCT 
ATGCCAGACC CTGGAGCTGT GCAGAGTACC TTGACAACCT CTCAGAACAT GGAGAAAATT 
GATGACCTTC TGGTGAGCCT TCAGGAGCAA GGCAATAACA TCTCCCATTC CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303606.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301881.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42240
Clone ID Related Accession (Same CDS sequence)	XM_009303606.2
Accession Version	XM_009303606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301881.1
CDS	245..4018
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303606.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303606.2

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGCAG ATGATAAGTC CTGGAAGGCA 
GAGGCTGACA TTGACATGGA GTTTTTCCAT CAGAATCATC TTATTGTCAA AGTCCCACCA 
TATCAAAATC CAGCTATCAC ATCTCCTATA TCTGTTGGTA TCTATGTTGT CACGAATGCT 
GGGCGTTCAC ATGATGTTCA ACAATTTACA TACACTCCAG AGCCAGTGGT TGATTCTTCT 
GTAAAAAAGG AGGACTTGCC TCCAGTGACA CCTTGTTGTT TTGAACAAAT TAAAGTAATT 
GATACTGCCG TGATGACACC AGGCTTACCT CTTGTGAAAC GTGAAGAGGC CACTCCGATG 
GAGGTCTCCG GTAACCCTTC GGACTTCAAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303607.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301882.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42241
Clone ID Related Accession (Same CDS sequence)	XM_009303607.2 , XM_009303607.3
Accession Version	XM_009303607.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGATG ATAAGTCCTG GAAGGCAGAG 
GCTGACATTG ACATGGAGTT TTTCCATCAG AATCATCTTA TTGTCAAAGT CCCACCATAT 
CAAAATCCAG CTATCACATC TCCTATATCT GTTGGTATCT ATGTTGTCAC GAATGCTGGG 
CGTTCACATG ATGTTCAACA ATTTACATAC ACTCCAGAGC CAGTGGTTGA TTCTTCTGTA 
AAAAAGGAGG ACTTGCCTCC AGTGACACCT TGTTGTTTTG AACAAATTAA AGTAATTGAT 
ACTGCCGTGA TGACACCAGG CTTACCTCTT GTGAAACGTG AAGAGGCCAC TCCGATGGAG 
GTCTCCGGTA ACCCTTCGGA CTTCAAGCAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301882.1
CDS	245..4018
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASDDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303607.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303607.2

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGATG ATAAGTCCTG GAAGGCAGAG 
GCTGACATTG ACATGGAGTT TTTCCATCAG AATCATCTTA TTGTCAAAGT CCCACCATAT 
CAAAATCCAG CTATCACATC TCCTATATCT GTTGGTATCT ATGTTGTCAC GAATGCTGGG 
CGTTCACATG ATGTTCAACA ATTTACATAC ACTCCAGAGC CAGTGGTTGA TTCTTCTGTA 
AAAAAGGAGG ACTTGCCTCC AGTGACACCT TGTTGTTTTG AACAAATTAA AGTAATTGAT 
ACTGCCGTGA TGACACCAGG CTTACCTCTT GTGAAACGTG AAGAGGCCAC TCCGATGGAG 
GTCTCCGGTA ACCCTTCGGA CTTCAAGCAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303607.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301882.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X5, mRNA.

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CloneID	ODa42241
Clone ID Related Accession (Same CDS sequence)	XM_009303607.2 , XM_009303607.3
Accession Version	XM_009303607.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303607.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGATG ATAAGTCCTG GAAGGCAGAG 
GCTGACATTG ACATGGAGTT TTTCCATCAG AATCATCTTA TTGTCAAAGT CCCACCATAT 
CAAAATCCAG CTATCACATC TCCTATATCT GTTGGTATCT ATGTTGTCAC GAATGCTGGG 
CGTTCACATG ATGTTCAACA ATTTACATAC ACTCCAGAGC CAGTGGTTGA TTCTTCTGTA 
AAAAAGGAGG ACTTGCCTCC AGTGACACCT TGTTGTTTTG AACAAATTAA AGTAATTGAT 
ACTGCCGTGA TGACACCAGG CTTACCTCTT GTGAAACGTG AAGAGGCCAC TCCGATGGAG 
GTCTCCGGTA ACCCTTCGGA CTTCAAGCAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301882.1
CDS	261..4034
Translation	MPSDFTSLFSADIDLDSPKSLYSKESVYDLLPKELQLPSSGVQSAAAMSQKSGGEAGPPPPAALAADASSASSAMSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASDDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303607.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303607.3

ORF Insert Sequence:

ATGCCCTCTG ATTTCACATC GCTTTTTAGC GCGGATATCG ACCTCGATTC GCCCAAGTCA 
CTCTACTCCA AAGAGTCAGT ATATGATCTT CTCCCGAAAG AGCTGCAGCT GCCGTCGTCT 
GGAGTGCAGA GCGCAGCCGC TATGAGTCAG AAAAGCGGAG GAGAGGCTGG ACCGCCGCCG 
CCAGCAGCTC TCGCCGCAGA TGCCTCGTCC GCCTCTTCTG CTATGAGCTC TGGAAGTCCT 
CGCAGTGCTT TTCCCACCTC AGCGTCAGAT CGAAGCACCG TACACGACAG CGGCTCATCA 
GGAGATGTCA GCAGTCGAAG GAAATCGGAG GCTGTGGAAA ACAAACCCTC TGGTCGAGAT 
AAAGGTGTCC AGCAGACCCA GATGACCCCC TCCAAACGGC CTGTCCTCAA CATCTCGCCT 
CCACCCGAAG ACCTGTTCGA TGACAGCCAC ATGTCCTGCC AGGATGATCT GCTTCTCGAC 
TCGGAGCAAA GCAGCAGCAT CTGGATTGAA AACTCAGTGT CTAACTTCAG CATTGTGAGC 
TCTAGTTCCT ACAACGACAA CACTGAAGTG CCCCGCAAGG CCCGCAAATG TACCCCGCGA 
CAGAAGCCTG GGCCGAAGCC GGTTTCTGCG GAAGAGGCCA GCATGGATGT CTTTGATGCA 
GACAGTGCCA AAGGCCCGCA CTTTGTGCTC TCACAACTCG GCCCAGACAG CAAGACCTGT 
TCCAAAGCAA GCTCTGATGG ACCACAAGTG TCTGCTCTCA AAGGTGGGGT ACTGTCTTGT 
CAGTATCCTC AGAAAGGTGA AGGGAAAGAA CTGAAGATCG TGGTGCAGCC TGAGACCCAG 
CACAGAGCGC GTTACCTGAC TGAAGGCAGC CGAGGGTCAG TCAAAGACCA AACACAGCAG 
GGCTTCCCTA CTATCAAGCT TGAGGGTGTG AAGGAGTCAG TGGTCATGCA GATCTTTGTG 
GGCAATGATG CCGGACGTGT GAAGCCTCAC GGGTTCTACC AGGCTTGCAG AGTCACCGGC 
CGAAACACAA CCGCCTGCAG GGAAGTGGAC ATCGAAGGCA CCACAGTCAT CGAAGTGCCA 
ATGGATCCCA GCAGCAACAT GACTTTACCT GTGGACTGTG TGGGGATCCT GAAGCTCAGG 
AATGCCGATG TGGAGGCCCG TATCGGGGTG GCCGGGTCTA AGAAGAAGAG CACCCGTGCC 
AGACTGGTGT TTCGAGTCAA CATCCCACGC CCCGATGGCT CTGCGCTCAC GTTACAGACC 
TCGTCGTCTC CCATACTGTG CACCCAGCCT GCAGGAGCAC CTGAGATTCT CAAGAAGAGT 
TTACACAGCT GTTCGGTTAA AGGGGGGAAT GAAGTCTTTA TCATTGGAAA AAACTTTCTG 
AAAGGAACCA AGGTCATATT TCAGGAGAAC GCTTCAGATG ATAAGTCCTG GAAGGCAGAG 
GCTGACATTG ACATGGAGTT TTTCCATCAG AATCATCTTA TTGTCAAAGT CCCACCATAT 
CAAAATCCAG CTATCACATC TCCTATATCT GTTGGTATCT ATGTTGTCAC GAATGCTGGG 
CGTTCACATG ATGTTCAACA ATTTACATAC ACTCCAGAGC CAGTGGTTGA TTCTTCTGTA 
AAAAAGGAGG ACTTGCCTCC AGTGACACCT TGTTGTTTTG AACAAATTAA AGTAATTGAT 
ACTGCCGTGA TGACACCAGG CTTACCTCTT GTGAAACGTG AAGAGGCCAC TCCGATGGAG 
GTCTCCGGTA ACCCTTCGGA CTTCAAGCAG ACCTCTGAAG TCATCAATTC AGCCCAGCAA 
GCTTTAGACA TGAGTACTGA GATTTCCACC AACAACTGCA AATTTTCCAG TCCGTTGGCA 
CACCCAACAA GAGAGCCAGA TCAATCTCAG AATTCAGTCT TTTCCAACAA AGAACCTTTC 
AACACCATCC AGGAACAGAA TCTTGCATCC AGTGGCTCTT TCCATGTGCC TAATAAATCT 
CTTCTCCAGC AAGGAGTTCA GCCATTCCTC CTGGAACACA GAGACCGTCT TGGACAGGAG 
ATGCCAGGCA GCAGTGGCAG TTCAGTAGTG GCTCTTTCCC AATTAGATGA CAATCCGCAG 
CAACAGCTCT CTCTTCTACC TCCAGATGAG GTGGCCCAAC TGGAGCAGGC CGTGCGGCAG 
CTGCAAGCGA AAGGATACTG CGGCATACAA CTCCAGAGTG AGAATTCACT GTCAAAGCAA 
AGACAACATC AGCTTCAACA GCAGCAACAG CAAATCCAAC AACAGCAGCA TCAAATCCAC 
CAACAACAGC AGCAAATTCA GCAACAGCAG CAGCACATCC AGCATCAAGA GCAGCAAATC 
CAGCTGCAGC AGCAGCACCA CCACCAGCAG CAAGCTCTGG AGAACCTTCA ACATCAGCTT 
TTTCAGCCCC AGGTTCAAAT TCACTGTAGT TCAGAGCAGC AGAGCTCTTC CCAGGGTATG 
GTGCAGAATG GAAGTTCCCT TTTCCAGTCC ACCCCATCCC AACAACAACA GCAGCAGCAA 
GCAACTTTGT TCCAACAAGG AGCTGGCTTT CTTCAGCAGC AACCCAGTCA TTCCTCTCCT 
TCCATGTTCC ACAACACTAC AGCAATGACC GAAGCTCAAA GCCCTCAGGA GTCTCTTTTC 
CACACACAAA AGACCTCTCC CAACCAGGAT CAGGTCCAAG CTTCCATTTT CCAAAGCAAT 
CTTTCGGTTC TCAGTGGGTC CAACATGTCT TCAGAAGCTC AGCCCTCTGC AGCTAGTATG 
TTTCTTCCCC AGAACACAGT ACCAGCTCAA CTTTCCGTTA ACGCCAACCA GCCACAGCAA 
CTGGCGTTCC TCACTTCCAT GCAGACATCT CTTGAGGCGC CATCTGTATT TCAGACTCCA 
ACCCAACTGT CCCCCATTCA GCAAGGCACT CCGATGGATC AGCAGCAGTC CCCACAGCAT 
GCCCAACCGG GTTCCATGTT CCAGAGCATC TCCCAATCAC CAAGCAAACT CACCCCTGGT 
CAACAGCAGC AGGCTGGTCT ACTCCTCGGA TCCTTAAACT CTCCAATCAC ACAAGAGCAA 
TCCTCCAATC TGTTATTCGG TGGCCAAGTA CAGATAGGAT CAATGAACAA TAGCAGCCTA 
GCTTCGTCAG AGCAACAGAA CACTCCCCTT CCCTTCGCTC AGGCTGGTCT GGTCACCGTG 
AGGCAGCAGG AGGCATCTGA ACCAATGACA TTTCAGGATC AGACAGCAAT GGTGGTCAAC 
CTGTCATCAA ATAAGCCCAT CTTCCAAGAC CAGCAGCCAA TGCAGTTGGC ACCAAGTTCT 
AATCCAGGCT CTTCACAGTC TGTAAATCTT TTCATGGCAC AGTCCAACAT GCAAGGGGGG 
ATGACTACAC AAGAAGCTCT TTTTGCACCA CAAGGAGATG TAGGTGGCAT TCAGACAAGC 
ACTTCCTCTC CAGTGCAACA GCCTGGTCAA CTCTTTCAGA CCACAATGAG TGGGAGTCTC 
AACCAGCCCA ACCAGGATCA GCAGACAGAC CTATTCCTCT TCGAGATCCA GAATGAGTGC 
CGTCAACTCA TGAACAGCCA AGGATCTACA CTGTCTAATC AAATAATTAC CATCAATCAG 
CCTGGACAGA GTCAGCAAGA GAGTGATGGG CAAATCCAGT CTTTACTTGG GCAGGCTATG 
CCAGACCCTG GAGCTGTGCA GAGTACCTTG ACAACCTCTC AGAACATGGA GAAAATTGAT 
GACCTTCTGG TGAGCCTTCA GGAGCAAGGC AATAACATCT CCCATTCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5b ( XM_009303608.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301883.1 Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.

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CloneID	ODa42242
Clone ID Related Accession (Same CDS sequence)	XM_009303608.2
Accession Version	XM_009303608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTG GAAGTCCTCG CAGTGCTTTT CCCACCTCAG CGTCAGATCG AAGCACCGTA 
CACGACAGCG GCTCATCAGG AGATGTCAGC AGTCGAAGGA AATCGGAGGC TGTGGAAAAC 
AAACCCTCTG GTCGAGATAA AGGTGTCCAG CAGACCCAGA TGACCCCCTC CAAACGGCCT 
GTCCTCAACA TCTCGCCTCC ACCCGAAGAC CTGTTCGATG ACAGCCACAT GTCCTGCCAG 
GATGATCTGC TTCTCGACTC GGAGCAAAGC AGCAGCATCT GGATTGAAAA CTCAGTGTCT 
AACTTCAGCA TTGTGAGCTC TAGTTCCTAC AACGACAACA CTGAAGTGCC CCGCAAGGCC 
CGCAAATGTA CCCCGCGACA GAAGCCTGGG CCGAAGCCGG TTTCTGCGGA AGAGGCCAGC 
ATGGATGTCT TTGATGCAGA CAGTGCCAAA GGCCCGCACT TTGTGCTCTC ACAACTCGGC 
CCAGACAGCA AGACCTGTTC CAAAGCAAGC TCTGATGGAC CACAAGTGTC TGCTCTCAAA 
GGTGGGGTAC TGTCTTGTCA GTATCCTCAG AAAGGTGAAG GGAAAGAACT GAAGATCGTG 
GTGCAGCCTG AGACCCAGCA CAGAGCGCGT TACCTGACTG AAGGCAGCCG AGGGTCAGTC 
AAAGACCAAA CACAGCAGGG CTTCCCTACT ATCAAGCTTG AGGGTGTGAA GGAGTCAGTG 
GTCATGCAGA TCTTTGTGGG CAATGATGCC GGACGTGTGA AGCCTCACGG GTTCTACCAG 
GCTTGCAGAG TCACCGGCCG AAACACAACC GCCTGCAGGG AAGTGGACAT CGAAGGCACC 
ACAGTCATCG AAGTGCCAAT GGATCCCAGC AGCAACATGA CTTTACCTGT GGACTGTGTG 
GGGATCCTGA AGCTCAGGAA TGCCGATGTG GAGGCCCGTA TCGGGGTGGC CGGGTCTAAG 
AAGAAGAGCA CCCGTGCCAG ACTGGTGTTT CGAGTCAACA TCCCACGCCC CGATGGCTCT 
GCGCTCACGT TACAGACCTC GTCGTCTCCC ATACTGTGCA CCCAGCCTGC AGGAGCACCT 
GAGATTCTCA AGAAGAGTTT ACACAGCTGT TCGGTTAAAG GGGGGAATGA AGTCTTTATC 
ATTGGAAAAA ACTTTCTGAA AGGAACCAAG GTCATATTTC AGGAGAACGC TTCAGCAGAT 
GATAAGTCCT GGAAGGCAGA GGCTGACATT GACATGGAGT TTTTCCATCA GAATCATCTT 
ATTGTCAAAG TCCCACCATA TCAAAATCCA GCTATCACAT CTCCTATATC TGTTGGTATC 
TATGTTGTCA CGAATGCTGG GCGTTCACAT GATGTTCAAC AATTTACATA CACTCCAGAG 
CCAGTGGTTG ATTCTTCTGT AAAAAAGGAG GACTTGCCTC CAGTGACACC TTGTTGTTTT 
GAACAAATTA AAGTAATTGA TACTGCCGTG ATGACACCAG GCTTACCTCT TGTGAAACGT 
GAAGAGGCCA CTCCGATGGA GGTCTCCGGT AACCCTTCGG ACTTCAAGCA GACCTCTGAA 
GTCATCAATT CAGCCCAGCA AGCTTTAGAC ATGAGTACTG AGATTTCCAC CAACAACTGC 
AAATTTTCCA GTCCGTTGGC ACACCCAACA AGAGAGCCAG ATCAATCTCA GAATTCAGTC 
TTTTCCAACA AAGAACCTTT CAACACCATC CAGGAACAGA ATCTTGCATC CAGTGGCTCT 
TTCCATGTGC CTAATAAATC TCTTCTCCAG CAAGGAGTTC AGCCATTCCT CCTGGAACAC 
AGAGACCGTC TTGGACAGGA GATGCCAGGC AGCAGTGGCA GTTCAGTAGT GGCTCTTTCC 
CAATTAGATG ACAATCCGCA GCAACAGCTC TCTCTTCTAC CTCCAGATGA GGTGGCCCAA 
CTGGAGCAGG CCGTGCGGCA GCTGCAAGCG AAAGGATACT GCGGCATACA ACTCCAGAGT 
GAGAATTCAC TGTCAAAGCA AAGACAACAT CAGCTTCAAC AGCAGCAACA GCAAATCCAA 
CAACAGCAGC ATCAAATCCA CCAACAACAG CAGCAAATTC AGCAACAGCA GCAGCACATC 
CAGCATCAAG AGCAGCAAAT CCAGCTGCAG CAGCAGCACC ACCACCAGCA GCAAGCTCTG 
GAGAACCTTC AACATCAGCT TTTTCAGCCC CAGGTTCAAA TTCACTGTAG TTCAGAGCAG 
CAGAGCTCTT CCCAGGGTAT GGTGCAGAAT GGAAGTTCCC TTTTCCAGTC CACCCCATCC 
CAACAACAAC AGCAGCAGCA AGCAACTTTG TTCCAACAAG GAGCTGGCTT TCTTCAGCAG 
CAACCCAGTC ATTCCTCTCC TTCCATGTTC CACAACACTA CAGCAATGAC CGAAGCTCAA 
AGCCCTCAGG AGTCTCTTTT CCACACACAA AAGACCTCTC CCAACCAGGA TCAGGTCCAA 
GCTTCCATTT TCCAAAGCAA TCTTTCGGTT CTCAGTGGGT CCAACATGTC TTCAGAAGCT 
CAGCCCTCTG CAGCTAGTAT GTTTCTTCCC CAGAACACAG TACCAGCTCA ACTTTCCGTT 
AACGCCAACC AGCCACAGCA ACTGGCGTTC CTCACTTCCA TGCAGACATC TCTTGAGGCG 
CCATCTGTAT TTCAGACTCC AACCCAACTG TCCCCCATTC AGCAAGGCAC TCCGATGGAT 
CAGCAGCAGT CCCCACAGCA TGCCCAACCG GGTTCCATGT TCCAGAGCAT CTCCCAATCA 
CCAAGCAAAC TCACCCCTGG TCAACAGCAG CAGGCTGGTC TACTCCTCGG ATCCTTAAAC 
TCTCCAATCA CACAAGAGCA ATCCTCCAAT CTGTTATTCG GTGGCCAAGT ACAGATAGGA 
TCAATGAACA ATAGCAGCCT AGCTTCGTCA GAGCAACAGA ACACTCCCCT TCCCTTCGCT 
CAGGCTGGTC TGGTCACCGT GAGGCAGCAG GAGGCATCTG AACCAATGAC ATTTCAGGAT 
CAGACAGCAA TGGTGGTCAA CCTGTCATCA AATAAGCCCA TCTTCCAAGA CCAGCAGCCA 
ATGCAGTTGG CACCAAGTTC TAATCCAGGC TCTTCACAGT CTGTAAATCT TTTCATGGCA 
CAGTCCAACA TGCAAGGGGG GATGACTACA CAAGAAGCTC TTTTTGCACC ACAAGGAGAT 
GTAGGTGGCA TTCAGACAAG CACTTCCTCT CCAGTGCAAC AGCCTGGTCA ACTCTTTCAG 
ACCACAATGA GTGGGAGTCT CAACCAGCCC AACCAGGATC AGCAGACAGA CCTATTCCTC 
TTCGAGATCC AGAATGAGTG CCGTCAACTC ATGAACAGCC AAGGATCTAC ACTGTCTAAT 
CAAATAATTA CCATCAATCA GCCTGGACAG AGTCAGCAAG AGAGTGATGG GCAAATCCAG 
TCTTTACTTG GGCAGGCTAT GCCAGACCCT GGAGCTGTGC AGAGTACCTT GACAACCTCT 
CAGAACATGG AGAAAATTGA TGACCTTCTG GTGAGCCTTC AGGAGCAAGG CAATAACATC 
TCCCATTCCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301883.1
CDS	176..3730
Translation	MSSGSPRSAFPTSASDRSTVHDSGSSGDVSSRRKSEAVENKPSGRDKGVQQTQMTPSKRPVLNISPPPEDLFDDSHMSCQDDLLLDSEQSSSIWIENSVSNFSIVSSSSYNDNTEVPRKARKCTPRQKPGPKPVSAEEASMDVFDADSAKGPHFVLSQLGPDSKTCSKASSDGPQVSALKGGVLSCQYPQKGEGKELKIVVQPETQHRARYLTEGSRGSVKDQTQQGFPTIKLEGVKESVVMQIFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVPMDPSSNMTLPVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIPRPDGSALTLQTSSSPILCTQPAGAPEILKKSLHSCSVKGGNEVFIIGKNFLKGTKVIFQENASADDKSWKAEADIDMEFFHQNHLIVKVPPYQNPAITSPISVGIYVVTNAGRSHDVQQFTYTPEPVVDSSVKKEDLPPVTPCCFEQIKVIDTAVMTPGLPLVKREEATPMEVSGNPSDFKQTSEVINSAQQALDMSTEISTNNCKFSSPLAHPTREPDQSQNSVFSNKEPFNTIQEQNLASSGSFHVPNKSLLQQGVQPFLLEHRDRLGQEMPGSSGSSVVALSQLDDNPQQQLSLLPPDEVAQLEQAVRQLQAKGYCGIQLQSENSLSKQRQHQLQQQQQQIQQQQHQIHQQQQQIQQQQQHIQHQEQQIQLQQQHHHQQQALENLQHQLFQPQVQIHCSSEQQSSSQGMVQNGSSLFQSTPSQQQQQQQATLFQQGAGFLQQQPSHSSPSMFHNTTAMTEAQSPQESLFHTQKTSPNQDQVQASIFQSNLSVLSGSNMSSEAQPSAASMFLPQNTVPAQLSVNANQPQQLAFLTSMQTSLEAPSVFQTPTQLSPIQQGTPMDQQQSPQHAQPGSMFQSISQSPSKLTPGQQQQAGLLLGSLNSPITQEQSSNLLFGGQVQIGSMNNSSLASSEQQNTPLPFAQAGLVTVRQQEASEPMTFQDQTAMVVNLSSNKPIFQDQQPMQLAPSSNPGSSQSVNLFMAQSNMQGGMTTQEALFAPQGDVGGIQTSTSSPVQQPGQLFQTTMSGSLNQPNQDQQTDLFLFEIQNECRQLMNSQGSTLSNQIITINQPGQSQQESDGQIQSLLGQAMPDPGAVQSTLTTSQNMEKIDDLLVSLQEQGNNISHSY

Target ORF information:

RefSeq Version	XM_009303608.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear factor of activated T-cells 5, tonicity-responsive b (nfat5b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303608.2

ORF Insert Sequence:

ATGAGCTCTG GAAGTCCTCG CAGTGCTTTT CCCACCTCAG CGTCAGATCG AAGCACCGTA 
CACGACAGCG GCTCATCAGG AGATGTCAGC AGTCGAAGGA AATCGGAGGC TGTGGAAAAC 
AAACCCTCTG GTCGAGATAA AGGTGTCCAG CAGACCCAGA TGACCCCCTC CAAACGGCCT 
GTCCTCAACA TCTCGCCTCC ACCCGAAGAC CTGTTCGATG ACAGCCACAT GTCCTGCCAG 
GATGATCTGC TTCTCGACTC GGAGCAAAGC AGCAGCATCT GGATTGAAAA CTCAGTGTCT 
AACTTCAGCA TTGTGAGCTC TAGTTCCTAC AACGACAACA CTGAAGTGCC CCGCAAGGCC 
CGCAAATGTA CCCCGCGACA GAAGCCTGGG CCGAAGCCGG TTTCTGCGGA AGAGGCCAGC 
ATGGATGTCT TTGATGCAGA CAGTGCCAAA GGCCCGCACT TTGTGCTCTC ACAACTCGGC 
CCAGACAGCA AGACCTGTTC CAAAGCAAGC TCTGATGGAC CACAAGTGTC TGCTCTCAAA 
GGTGGGGTAC TGTCTTGTCA GTATCCTCAG AAAGGTGAAG GGAAAGAACT GAAGATCGTG 
GTGCAGCCTG AGACCCAGCA CAGAGCGCGT TACCTGACTG AAGGCAGCCG AGGGTCAGTC 
AAAGACCAAA CACAGCAGGG CTTCCCTACT ATCAAGCTTG AGGGTGTGAA GGAGTCAGTG 
GTCATGCAGA TCTTTGTGGG CAATGATGCC GGACGTGTGA AGCCTCACGG GTTCTACCAG 
GCTTGCAGAG TCACCGGCCG AAACACAACC GCCTGCAGGG AAGTGGACAT CGAAGGCACC 
ACAGTCATCG AAGTGCCAAT GGATCCCAGC AGCAACATGA CTTTACCTGT GGACTGTGTG 
GGGATCCTGA AGCTCAGGAA TGCCGATGTG GAGGCCCGTA TCGGGGTGGC CGGGTCTAAG 
AAGAAGAGCA CCCGTGCCAG ACTGGTGTTT CGAGTCAACA TCCCACGCCC CGATGGCTCT 
GCGCTCACGT TACAGACCTC GTCGTCTCCC ATACTGTGCA CCCAGCCTGC AGGAGCACCT 
GAGATTCTCA AGAAGAGTTT ACACAGCTGT TCGGTTAAAG GGGGGAATGA AGTCTTTATC 
ATTGGAAAAA ACTTTCTGAA AGGAACCAAG GTCATATTTC AGGAGAACGC TTCAGCAGAT 
GATAAGTCCT GGAAGGCAGA GGCTGACATT GACATGGAGT TTTTCCATCA GAATCATCTT 
ATTGTCAAAG TCCCACCATA TCAAAATCCA GCTATCACAT CTCCTATATC TGTTGGTATC 
TATGTTGTCA CGAATGCTGG GCGTTCACAT GATGTTCAAC AATTTACATA CACTCCAGAG 
CCAGTGGTTG ATTCTTCTGT AAAAAAGGAG GACTTGCCTC CAGTGACACC TTGTTGTTTT 
GAACAAATTA AAGTAATTGA TACTGCCGTG ATGACACCAG GCTTACCTCT TGTGAAACGT 
GAAGAGGCCA CTCCGATGGA GGTCTCCGGT AACCCTTCGG ACTTCAAGCA GACCTCTGAA 
GTCATCAATT CAGCCCAGCA AGCTTTAGAC ATGAGTACTG AGATTTCCAC CAACAACTGC 
AAATTTTCCA GTCCGTTGGC ACACCCAACA AGAGAGCCAG ATCAATCTCA GAATTCAGTC 
TTTTCCAACA AAGAACCTTT CAACACCATC CAGGAACAGA ATCTTGCATC CAGTGGCTCT 
TTCCATGTGC CTAATAAATC TCTTCTCCAG CAAGGAGTTC AGCCATTCCT CCTGGAACAC 
AGAGACCGTC TTGGACAGGA GATGCCAGGC AGCAGTGGCA GTTCAGTAGT GGCTCTTTCC 
CAATTAGATG ACAATCCGCA GCAACAGCTC TCTCTTCTAC CTCCAGATGA GGTGGCCCAA 
CTGGAGCAGG CCGTGCGGCA GCTGCAAGCG AAAGGATACT GCGGCATACA ACTCCAGAGT 
GAGAATTCAC TGTCAAAGCA AAGACAACAT CAGCTTCAAC AGCAGCAACA GCAAATCCAA 
CAACAGCAGC ATCAAATCCA CCAACAACAG CAGCAAATTC AGCAACAGCA GCAGCACATC 
CAGCATCAAG AGCAGCAAAT CCAGCTGCAG CAGCAGCACC ACCACCAGCA GCAAGCTCTG 
GAGAACCTTC AACATCAGCT TTTTCAGCCC CAGGTTCAAA TTCACTGTAG TTCAGAGCAG 
CAGAGCTCTT CCCAGGGTAT GGTGCAGAAT GGAAGTTCCC TTTTCCAGTC CACCCCATCC 
CAACAACAAC AGCAGCAGCA AGCAACTTTG TTCCAACAAG GAGCTGGCTT TCTTCAGCAG 
CAACCCAGTC ATTCCTCTCC TTCCATGTTC CACAACACTA CAGCAATGAC CGAAGCTCAA 
AGCCCTCAGG AGTCTCTTTT CCACACACAA AAGACCTCTC CCAACCAGGA TCAGGTCCAA 
GCTTCCATTT TCCAAAGCAA TCTTTCGGTT CTCAGTGGGT CCAACATGTC TTCAGAAGCT 
CAGCCCTCTG CAGCTAGTAT GTTTCTTCCC CAGAACACAG TACCAGCTCA ACTTTCCGTT 
AACGCCAACC AGCCACAGCA ACTGGCGTTC CTCACTTCCA TGCAGACATC TCTTGAGGCG 
CCATCTGTAT TTCAGACTCC AACCCAACTG TCCCCCATTC AGCAAGGCAC TCCGATGGAT 
CAGCAGCAGT CCCCACAGCA TGCCCAACCG GGTTCCATGT TCCAGAGCAT CTCCCAATCA 
CCAAGCAAAC TCACCCCTGG TCAACAGCAG CAGGCTGGTC TACTCCTCGG ATCCTTAAAC 
TCTCCAATCA CACAAGAGCA ATCCTCCAAT CTGTTATTCG GTGGCCAAGT ACAGATAGGA 
TCAATGAACA ATAGCAGCCT AGCTTCGTCA GAGCAACAGA ACACTCCCCT TCCCTTCGCT 
CAGGCTGGTC TGGTCACCGT GAGGCAGCAG GAGGCATCTG AACCAATGAC ATTTCAGGAT 
CAGACAGCAA TGGTGGTCAA CCTGTCATCA AATAAGCCCA TCTTCCAAGA CCAGCAGCCA 
ATGCAGTTGG CACCAAGTTC TAATCCAGGC TCTTCACAGT CTGTAAATCT TTTCATGGCA 
CAGTCCAACA TGCAAGGGGG GATGACTACA CAAGAAGCTC TTTTTGCACC ACAAGGAGAT 
GTAGGTGGCA TTCAGACAAG CACTTCCTCT CCAGTGCAAC AGCCTGGTCA ACTCTTTCAG 
ACCACAATGA GTGGGAGTCT CAACCAGCCC AACCAGGATC AGCAGACAGA CCTATTCCTC 
TTCGAGATCC AGAATGAGTG CCGTCAACTC ATGAACAGCC AAGGATCTAC ACTGTCTAAT 
CAAATAATTA CCATCAATCA GCCTGGACAG AGTCAGCAAG AGAGTGATGG GCAAATCCAG 
TCTTTACTTG GGCAGGCTAT GCCAGACCCT GGAGCTGTGC AGAGTACCTT GACAACCTCT 
CAGAACATGG AGAAAATTGA TGACCTTCTG GTGAGCCTTC AGGAGCAAGG CAATAACATC 
TCCCATTCCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nfat5b
Entrez Gene ID	555802
Full Name	nuclear factor of activated T cells 5b
General protein information	Preferred Names nuclear factor of activated T cells 5b Names nuclear factor of activated T-cells 5 nuclear factor of activated T-cells 5, tonicity-responsive b zmp:0000001047
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	NFAT5	XP_001093880.2
Mus musculus (house mouse)	Nfat5	NP_061293.2
Xenopus tropicalis (tropical clawed frog)	nfat5	XP_002937623.2
Homo sapiens (human)	NFAT5	NP_619727.2
Pan troglodytes (chimpanzee)	NFAT5	XP_001168930.2
Canis lupus familiaris (dog)	NFAT5	XP_005620824.1
Gallus gallus (chicken)	NFAT5	NP_001185929.1
Bos taurus (cattle)	NFAT5	XP_002694885.1
Rattus norvegicus (Norway rat)	Nfat5	NP_001100895.1
Danio rerio (zebrafish)	LOC555802	XP_005174610.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nfat5b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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