Nelfa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfa
Entrez Gene ID	305455
Full Name	negative elongation factor complex member A
Synonyms	Whsc2
General protein information	Preferred Names negative elongation factor complex member A Names negative elongation factor A Wolf-Hirschhorn syndrome candidate 2 protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008339.1	NP_001008340.1	negative elongation factor A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	nelfa	XP_002936807.1
Homo sapiens (human)	NELFA	NP_005654.3
Pan troglodytes (chimpanzee)	WHSC2	NP_001231577.1
Canis lupus familiaris (dog)	NELFA	XP_545924.2
Bos taurus (cattle)	WHSC2	NP_001095823.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002290	XP_001687824.2
	WHSC2	XP_001100646.2
Danio rerio (zebrafish)	nelfa	NP_001166972.1
Mus musculus (house mouse)	Nelfa	NP_036044.1
Rattus norvegicus (Norway rat)	Nelfa	NP_001008340.1
Gallus gallus (chicken)	WHSC2	NP_001026338.1

Gene Ontology
Bibliography

Related articles in PubMed

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
Luo M, Lu X, Zhu R, Zhang Z, Chow CC, Li R, Simons SS Jr
The Journal of biological chemistry288(47)34055-72(2013 Nov)

Control of transcriptional elongation.
Kwak H, Lis JT
Annual review of genetics47483-508(2013)

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Flynn RA, Almada AE, Zamudio JR, Sharp PA
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)

Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II.
Saha RN, Wissink EM, Bailey ER, Zhao M, Fargo DC, Hwang JY, Daigle KR, Fenn JD, Adelman K, Dudek SM
Nature neuroscience14(7)848-56(2011 May)

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS
Cell stem cell5(4)420-33(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.
Title: A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.

The following Nelfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11576	NM_001008339.1 Latest version!	Rattus norvegicus negative elongation factor complex member A (Nelfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$314.30 $449.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfa ( NM_001008339.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008340.1 Rattus norvegicus negative elongation factor complex member A (Nelfa), mRNA.

Price & Availability↑

CloneID	ORa11576
Clone ID Related Accession (Same CDS sequence)	NM_001008339.1
Accession Version	NM_001008339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085948.1. On Dec 16, 2004 this sequence version replaced XM_223539.2. ##Evidence-Data-START## Transcript exon combination :: BC085948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCCA TGCGGGAGAG CGACACGGGC CTGTGGCTGC ACAACAAACT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCTGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCATCG GCTGTGAAGC TCAAGCTGCT GCTCGGGACG 
CTGCATCTTC CGCGCCGCAC GGTGGATGAG ATGAAGGGCG CTCTGATGGA TATCATCCAG 
CTGGCCACTC TGGACTCAGA TCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACAG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CGGCCATGCT ACCGTTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GTCCCCTCAC CCCGCCAGTG 
AAACATTTTC AGCTGAAGCG GAAACCTAAG AGTGCCACAC TGCGGGCAGA ACTGCTACAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGGAGTGCTG GGGTCCCATT CCACGCCAAG 
GGCCGGGGGC TGCTCCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT CCCGAAGCAA 
GCCCCCTTCA GAAGTCCCAC CACACCCAGT GTCTTCAGCC CTTCTGGGAA CCGCACTCCA 
ATACCACCTT CAAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGCCGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAATGCCAC CCCTGACTAT 
GCTGCTGGCC TGGTGTCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCCTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCCCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCCCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGTCCAG CGACCCCAAC ACCTGCAGCA CCTGCCTCAC CTCTGACACC TACTGCTCCC 
CCAGCTGTCA CTCCCACTGC TCAGACACCC CCAGTGGCCA TGGTGGCCCC ACAGACCCAG 
GCCCCGGCCC CTGTTCAGCA GCAACCTAAG AAGAACCTGT CCCTCACGAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACAGCA AACAAGGTCA CTCGGCCAGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAA AACCCATGCC CGGAGCAAGG GGATGTGATC 
CAGATCAAGC TCAGCGAGCA CACAGAGGAC CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACGGT GTTCGAGATG AACTATGCCA CAGGCCAGTG GACGCGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008340.1
CDS	60..1652
Translation	MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHRLSSAVKLKLLLGTLHLPRRTVDEMKGALMDIIQLATLDSDPWVLMVADILKSFPDTGSLNLDLEEQNPNVQDILGELREKVSECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTTPSVFSPSGNRTPIPPSRTPLQKERGVKLLDISELNTVGAGREAKRRRKTLDTEVVEKPTKEETVVENATPDYAAGLVSTQKLGSLNSEPTLPSTSYLPSTPSVVPASSYIPSSETPPAPPSREASRPPEEPSAPSPTLPTQFKQRAPMYNSGLSPATPTPAAPASPLTPTAPPAVTPTAQTPPVAMVAPQTQAPAPVQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCPEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	NM_001008339.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member A (Nelfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008339.1

ORF Insert Sequence:

ATGGCTTCCA TGCGGGAGAG CGACACGGGC CTGTGGCTGC ACAACAAACT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCTGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCATCG GCTGTGAAGC TCAAGCTGCT GCTCGGGACG 
CTGCATCTTC CGCGCCGCAC GGTGGATGAG ATGAAGGGCG CTCTGATGGA TATCATCCAG 
CTGGCCACTC TGGACTCAGA TCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACAG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CGGCCATGCT ACCGTTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GTCCCCTCAC CCCGCCAGTG 
AAACATTTTC AGCTGAAGCG GAAACCTAAG AGTGCCACAC TGCGGGCAGA ACTGCTACAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGGAGTGCTG GGGTCCCATT CCACGCCAAG 
GGCCGGGGGC TGCTCCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT CCCGAAGCAA 
GCCCCCTTCA GAAGTCCCAC CACACCCAGT GTCTTCAGCC CTTCTGGGAA CCGCACTCCA 
ATACCACCTT CAAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGCCGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAATGCCAC CCCTGACTAT 
GCTGCTGGCC TGGTGTCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCCTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCCCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCCCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGTCCAG CGACCCCAAC ACCTGCAGCA CCTGCCTCAC CTCTGACACC TACTGCTCCC 
CCAGCTGTCA CTCCCACTGC TCAGACACCC CCAGTGGCCA TGGTGGCCCC ACAGACCCAG 
GCCCCGGCCC CTGTTCAGCA GCAACCTAAG AAGAACCTGT CCCTCACGAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACAGCA AACAAGGTCA CTCGGCCAGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAA AACCCATGCC CGGAGCAAGG GGATGTGATC 
CAGATCAAGC TCAGCGAGCA CACAGAGGAC CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACGGT GTTCGAGATG AACTATGCCA CAGGCCAGTG GACGCGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
The Journal of biological chemistry288(47)34055-72(2013 Nov)
Luo M,Lu X,Zhu R,Zhang Z,Chow CC,Li R,Simons SS Jr

Control of transcriptional elongation.
Annual review of genetics47483-508(2013)
Kwak H,Lis JT

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)
Flynn RA,Almada AE,Zamudio JR,Sharp PA

Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II.
Nature neuroscience14(7)848-56(2011 May)
Saha RN,Wissink EM,Bailey ER,Zhao M,Fargo DC,Hwang JY,Daigle KR,Fenn JD,Adelman K,Dudek SM

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Cell stem cell5(4)420-33(2009 Oct)
Nishiyama A,Xin L,Sharov AA,Thomas M,Mowrer G,Meyers E,Piao Y,Mehta S,Yee S,Nakatake Y,Stagg C,Sharova L,Correa-Cerro LS,Bassey U,Hoang H,Kim E,Tapnio R,Qian Y,Dudekula D,Zalzman M,Li M,Falco G,Yang HT,Lee SL,Monti M,Stanghellini I,Islam MN,Nagaraja R,Goldberg I,Wang W,Longo DL,Schlessinger D,Ko MS

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Nelfa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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