NELFA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFA
Entrez Gene ID	7469
Full Name	negative elongation factor complex member A
Synonyms	NELF-A,P/OKcl.15,WHSC2
General protein information	Preferred Names negative elongation factor complex member A Names negative elongation factor A wolf-Hirschhorn syndrome candidate 2 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008].
Disorder MIM:	606026

mRNA and Protein(s)

mRNA	Protein	Name
NM_005663.4	NP_005654.3	negative elongation factor A
XM_017008589.1	XP_016864078.1	negative elongation factor A isoform X1
NM_005663.5	NP_005654.4	negative elongation factor A
XM_017008589.2	XP_016864078.1	negative elongation factor A isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	nelfa	XP_002936807.1
Homo sapiens (human)	NELFA	NP_005654.3
Pan troglodytes (chimpanzee)	WHSC2	NP_001231577.1
Canis lupus familiaris (dog)	NELFA	XP_545924.2
Bos taurus (cattle)	WHSC2	NP_001095823.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002290	XP_001687824.2
	WHSC2	XP_001100646.2
Danio rerio (zebrafish)	nelfa	NP_001166972.1
Mus musculus (house mouse)	Nelfa	NP_036044.1
Rattus norvegicus (Norway rat)	Nelfa	NP_001008340.1
Gallus gallus (chicken)	WHSC2	NP_001026338.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gand?a J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morat? X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Pr?ulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I
Molecular systems biology13(3)918(2017 03)

Architecture and RNA binding of the human negative elongation factor.
Vos SM, P?llmann D, Caizzi L, Hofmann KB, Rombaut P, Zimniak T, Herzog F, Cramer P
eLife5(2016 06)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
Luo M, Lu X, Zhu R, Zhang Z, Chow CC, Li R, Simons SS Jr
The Journal of biological chemistry288(47)34055-72(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.
Title: Architecture and RNA binding of the human negative elongation factor.

These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.
Title: A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.

haploinsufficiency of SLBP and/or WHSC2 (NELF-A) contributes to several novel cellular phenotypes of WHS.
Title: Characterizing the functional consequences of haploinsufficiency of NELF-A (WHSC2) and SLBP identifies novel cellular phenotypes in Wolf-Hirschhorn syndrome.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei
Title: Cellular dynamics of the negative transcription elongation factor NELF.

The following NELFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20772	NM_005663.4 Latest version!	Homo sapiens negative elongation factor complex member A (NELFA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00
OHu61324	XM_017008589.2 Latest version!	Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu107765	NM_005663.5 Latest version!	Homo sapiens negative elongation factor complex member A (NELFA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu61324	XM_017008589.1	Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFA ( NM_005663.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005654.3 Homo sapiens negative elongation factor complex member A (NELFA), mRNA.

Price & Availability↑

CloneID	OHu20772
Clone ID Related Accession (Same CDS sequence)	NM_005663.4
Accession Version	NM_005663.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	negative elongation factor A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB082393.1, BC002764.2, AB044549.1, AL132868.24 and AI218582.1. This sequence is a reference standard in the RefSeqGene project. On Aug 4, 2011 this sequence version replaced NM_005663.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.195449.1, AK001304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGATGAAGG GCGCCCTAAT GGAGATCATC CAGCTCGCCA GCCTCGACTC GGACCCCTGG 
GTGCTCATGG TCGCCGACAT CTTGAAGTCC TTTCCGGACA CAGGCTCGCT TAACCTGGAG 
CTGGAGGAGC AGAATCCCAA CGTTCAGGAT ATTTTGGGAG AACTTAGAGA AAAGGTGGGT 
GAGTGTGAAG CGTCTGCCAT GCTGCCACTG GAGTGCCAGT ACTTGAACAA AAACGCCCTG 
ACGACCCTCG CGGGACCCCT CACTCCCCCG GTGAAGCATT TTCAGTTAAA GCGGAAACCC 
AAGAGCGCCA CGCTGCGGGC GGAGCTGCTG CAGAAGTCCA CGGAGACCGC CCAGCAGTTG 
AAGCGGAGCG CCGGGGTGCC CTTCCACGCC AAGGGCCGGG GGCTGCTGCG GAAGATGGAC 
ACCACCACCC CACTCAAAGG CATCCCGAAG CAGGCGCCCT TCAGAAGCCC CACGGCGCCC 
AGCGTCTTCA GCCCCACAGG GAACCGGACC CCCATCCCGC CTTCCAGGAC GCTGCTGCGG 
AAGGAACGAG GTGTGAAGCT GCTGGACATC TCTGAGCTGG ATATGGTTGG CGCTGGCCGA 
GAGGCGAAGC GGAGAAGGAA GACTCTCGAT GCGGAGGTGG TGGAGAAGCC GGCCAAGGAG 
GAAACGGTGG TGGAGAACGC CACCCCGGAC TACGCAGCCG GCCTGGTGTC CACGCAGAAA 
CTTGGGTCCC TGAACAATGA GCCTGCGCTG CCCTCCACGA GCTACCTTCC CTCCACGCCC 
AGCGTGGTTC CCGCCTCCTC CTACATCCCC AGCTCCGAGA CGCCCCCAGC CCCATCTTCC 
CGGGAAGCCA GCCGCCCACC AGAGGAGCCC AGCGCCCCGA GCCCCACGTT GCCAGCGCAG 
TTCAAGCAGC GGGCGCCCAT GTACAACAGC GGCCTGAGCC CTGCCACACC CACGCCTGCG 
GCGCCCACCT CGCCTCTGAC ACCCACCACA CCTCCGGCTG TCGCCCCTAC CACTCAGACA 
CCCCCGGTTG CCATGGTGGC CCCGCAGACC CAGGCCCCTG CTCAGCAGCA GCCTAAGAAG 
AACCTGTCCC TCACGAGAGA GCAGATGTTC GCTGCCCAGG AGATGTTCAA GACGGCCAAC 
AAAGTCACGC GGCCCGAGAA GGCCCTCATC CTGGGCTTCA TGGCCGGCTC CCGAGAGAAC 
CCGTGCCAGG AGCAGGGGGA CGTGATCCAG ATCAAGCTGA GCGAGCACAC GGAGGACCTG 
CCCAAGGCGG ACGGCCAGGG TAGCACAACC ATGCTGGTGG ACACAGTGTT TGAGATGAAC 
TATGCCACGG GCCAGTGGAC GCGCTTCAAG AAGTACAAGC CCATGACCAA TGTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005654.3
CDS	244..1863
Translation	MPGQRRALSPKMASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	NM_005663.4
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member A (NELFA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005663.4

ORF Insert Sequence:

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGATGAAGG GCGCCCTAAT GGAGATCATC CAGCTCGCCA GCCTCGACTC GGACCCCTGG 
GTGCTCATGG TCGCCGACAT CTTGAAGTCC TTTCCGGACA CAGGCTCGCT TAACCTGGAG 
CTGGAGGAGC AGAATCCCAA CGTTCAGGAT ATTTTGGGAG AACTTAGAGA AAAGGTGGGT 
GAGTGTGAAG CGTCTGCCAT GCTGCCACTG GAGTGCCAGT ACTTGAACAA AAACGCCCTG 
ACGACCCTCG CGGGACCCCT CACTCCCCCG GTGAAGCATT TTCAGTTAAA GCGGAAACCC 
AAGAGCGCCA CGCTGCGGGC GGAGCTGCTG CAGAAGTCCA CGGAGACCGC CCAGCAGTTG 
AAGCGGAGCG CCGGGGTGCC CTTCCACGCC AAGGGCCGGG GGCTGCTGCG GAAGATGGAC 
ACCACCACCC CACTCAAAGG CATCCCGAAG CAGGCGCCCT TCAGAAGCCC CACGGCGCCC 
AGCGTCTTCA GCCCCACAGG GAACCGGACC CCCATCCCGC CTTCCAGGAC GCTGCTGCGG 
AAGGAACGAG GTGTGAAGCT GCTGGACATC TCTGAGCTGG ATATGGTTGG CGCTGGCCGA 
GAGGCGAAGC GGAGAAGGAA GACTCTCGAT GCGGAGGTGG TGGAGAAGCC GGCCAAGGAG 
GAAACGGTGG TGGAGAACGC CACCCCGGAC TACGCAGCCG GCCTGGTGTC CACGCAGAAA 
CTTGGGTCCC TGAACAATGA GCCTGCGCTG CCCTCCACGA GCTACCTTCC CTCCACGCCC 
AGCGTGGTTC CCGCCTCCTC CTACATCCCC AGCTCCGAGA CGCCCCCAGC CCCATCTTCC 
CGGGAAGCCA GCCGCCCACC AGAGGAGCCC AGCGCCCCGA GCCCCACGTT GCCAGCGCAG 
TTCAAGCAGC GGGCGCCCAT GTACAACAGC GGCCTGAGCC CTGCCACACC CACGCCTGCG 
GCGCCCACCT CGCCTCTGAC ACCCACCACA CCTCCGGCTG TCGCCCCTAC CACTCAGACA 
CCCCCGGTTG CCATGGTGGC CCCGCAGACC CAGGCCCCTG CTCAGCAGCA GCCTAAGAAG 
AACCTGTCCC TCACGAGAGA GCAGATGTTC GCTGCCCAGG AGATGTTCAA GACGGCCAAC 
AAAGTCACGC GGCCCGAGAA GGCCCTCATC CTGGGCTTCA TGGCCGGCTC CCGAGAGAAC 
CCGTGCCAGG AGCAGGGGGA CGTGATCCAG ATCAAGCTGA GCGAGCACAC GGAGGACCTG 
CCCAAGGCGG ACGGCCAGGG TAGCACAACC ATGCTGGTGG ACACAGTGTT TGAGATGAAC 
TATGCCACGG GCCAGTGGAC GCGCTTCAAG AAGTACAAGC CCATGACCAA TGTGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFA ( XM_017008589.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864078.1 Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61324
Clone ID Related Accession (Same CDS sequence)	XM_017008589.1 , XM_017008589.2
Accession Version	XM_017008589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	negative elongation factor A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGACCCCGG CCGTCCGGAG CGCTCTGTGT CCGTTTTACT CTTTTCTACA CGCCGTTTTG 
CGTCATTCCA ATAAGTGGGT GTGTGAGATG AAGGGCGCCC TAATGGAGAT CATCCAGCTC 
GCCAGCCTCG ACTCGGACCC CTGGGTGCTC ATGGTCGCCG ACATCTTGAA GTCCTTTCCG 
GACACAGGCT CGCTTAACCT GGAGCTGGAG GAGCAGAATC CCAACGTTCA GGATATTTTG 
GGAGAACTTA GAGAAAAGGT GGGTGAGTGT GAAGCGTCTG CCATGCTGCC ACTGGAGTGC 
CAGTACTTGA ACAAAAACGC CCTGACGACC CTCGCGGGAC CCCTCACTCC CCCGGTGAAG 
CATTTTCAGT TAAAGCGGAA ACCCAAGAGC GCCACGCTGC GGGCGGAGCT GCTGCAGAAG 
TCCACGGAGA CCGCCCAGCA GTTGAAGCGG AGCGCCGGGG TGCCCTTCCA CGCCAAGGGC 
CGGGGGCTGC TGCGGAAGAT GGACACCACC ACCCCACTCA AAGGCATCCC GAAGCAGGCG 
CCCTTCAGAA GCCCCACGGC GCCCAGCGTC TTCAGCCCCA CAGGGAACCG GACCCCCATC 
CCGCCTTCCA GGACGCTGCT GCGGAAGGAA CGAGGTGTGA AGCTGCTGGA CATCTCTGAG 
CTGGATATGG TTGGCGCTGG CCGAGAGGCG AAGCGGAGAA GGAAGACTCT CGATGCGGAG 
GTGGTGGAGA AGCCGGCCAA GGAGGAAACG GTGGTGGAGA ACGCCACCCC GGACTACGCA 
GCCGGCCTGG TGTCCACGCA GAAACTTGGG TCCCTGAACA ATGAGCCTGC GCTGCCCTCC 
ACGAGCTACC TTCCCTCCAC GCCCAGCGTG GTTCCCGCCT CCTCCTACAT CCCCAGCTCC 
GAGACGCCCC CAGCCCCATC TTCCCGGGAA GCCAGCCGCC CACCAGAGGA GCCCAGCGCC 
CCGAGCCCCA CGTTGCCAGC GCAGTTCAAG CAGCGGGCGC CCATGTACAA CAGCGGCCTG 
AGCCCTGCCA CACCCACGCC TGCGGCGCCC ACCTCGCCTC TGACACCCAC CACACCTCCG 
GCTGTCGCCC CTACCACTCA GACACCCCCG GTTGCCATGG TGGCCCCGCA GACCCAGGCC 
CCTGCTCAGC AGCAGCCTAA GAAGAACCTG TCCCTCACGA GAGAGCAGAT GTTCGCTGCC 
CAGGAGATGT TCAAGACGGC CAACAAAGTC ACGCGGCCCG AGAAGGCCCT CATCCTGGGC 
TTCATGGCCG GCTCCCGAGA GAACCCGTGC CAGGAGCAGG GGGACGTGAT CCAGATCAAG 
CTGAGCGAGC ACACGGAGGA CCTGCCCAAG GCGGACGGCC AGGGTAGCAC AACCATGCTG 
GTGGACACAG TGTTTGAGAT GAACTATGCC ACGGGCCAGT GGACGCGCTT CAAGAAGTAC 
AAGCCCATGA CCAATGTGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864078.1
CDS	981..2684
Translation	MPGQRRALSPKMASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDETPAVRSALCPFYSFLHAVLRHSNKWVCEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	XM_017008589.1
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008589.1

ORF Insert Sequence:

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGACCCCGG CCGTCCGGAG CGCTCTGTGT CCGTTTTACT CTTTTCTACA CGCCGTTTTG 
CGTCATTCCA ATAAGTGGGT GTGTGAGATG AAGGGCGCCC TAATGGAGAT CATCCAGCTC 
GCCAGCCTCG ACTCGGACCC CTGGGTGCTC ATGGTCGCCG ACATCTTGAA GTCCTTTCCG 
GACACAGGCT CGCTTAACCT GGAGCTGGAG GAGCAGAATC CCAACGTTCA GGATATTTTG 
GGAGAACTTA GAGAAAAGGT GGGTGAGTGT GAAGCGTCTG CCATGCTGCC ACTGGAGTGC 
CAGTACTTGA ACAAAAACGC CCTGACGACC CTCGCGGGAC CCCTCACTCC CCCGGTGAAG 
CATTTTCAGT TAAAGCGGAA ACCCAAGAGC GCCACGCTGC GGGCGGAGCT GCTGCAGAAG 
TCCACGGAGA CCGCCCAGCA GTTGAAGCGG AGCGCCGGGG TGCCCTTCCA CGCCAAGGGC 
CGGGGGCTGC TGCGGAAGAT GGACACCACC ACCCCACTCA AAGGCATCCC GAAGCAGGCG 
CCCTTCAGAA GCCCCACGGC GCCCAGCGTC TTCAGCCCCA CAGGGAACCG GACCCCCATC 
CCGCCTTCCA GGACGCTGCT GCGGAAGGAA CGAGGTGTGA AGCTGCTGGA CATCTCTGAG 
CTGGATATGG TTGGCGCTGG CCGAGAGGCG AAGCGGAGAA GGAAGACTCT CGATGCGGAG 
GTGGTGGAGA AGCCGGCCAA GGAGGAAACG GTGGTGGAGA ACGCCACCCC GGACTACGCA 
GCCGGCCTGG TGTCCACGCA GAAACTTGGG TCCCTGAACA ATGAGCCTGC GCTGCCCTCC 
ACGAGCTACC TTCCCTCCAC GCCCAGCGTG GTTCCCGCCT CCTCCTACAT CCCCAGCTCC 
GAGACGCCCC CAGCCCCATC TTCCCGGGAA GCCAGCCGCC CACCAGAGGA GCCCAGCGCC 
CCGAGCCCCA CGTTGCCAGC GCAGTTCAAG CAGCGGGCGC CCATGTACAA CAGCGGCCTG 
AGCCCTGCCA CACCCACGCC TGCGGCGCCC ACCTCGCCTC TGACACCCAC CACACCTCCG 
GCTGTCGCCC CTACCACTCA GACACCCCCG GTTGCCATGG TGGCCCCGCA GACCCAGGCC 
CCTGCTCAGC AGCAGCCTAA GAAGAACCTG TCCCTCACGA GAGAGCAGAT GTTCGCTGCC 
CAGGAGATGT TCAAGACGGC CAACAAAGTC ACGCGGCCCG AGAAGGCCCT CATCCTGGGC 
TTCATGGCCG GCTCCCGAGA GAACCCGTGC CAGGAGCAGG GGGACGTGAT CCAGATCAAG 
CTGAGCGAGC ACACGGAGGA CCTGCCCAAG GCGGACGGCC AGGGTAGCAC AACCATGCTG 
GTGGACACAG TGTTTGAGAT GAACTATGCC ACGGGCCAGT GGACGCGCTT CAAGAAGTAC 
AAGCCCATGA CCAATGTGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFA ( XM_017008589.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864078.1 Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61324
Clone ID Related Accession (Same CDS sequence)	XM_017008589.1 , XM_017008589.2
Accession Version	XM_017008589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	negative elongation factor A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGACCCCGG CCGTCCGGAG CGCTCTGTGT CCGTTTTACT CTTTTCTACA CGCCGTTTTG 
CGTCATTCCA ATAAGTGGGT GTGTGAGATG AAGGGCGCCC TAATGGAGAT CATCCAGCTC 
GCCAGCCTCG ACTCGGACCC CTGGGTGCTC ATGGTCGCCG ACATCTTGAA GTCCTTTCCG 
GACACAGGCT CGCTTAACCT GGAGCTGGAG GAGCAGAATC CCAACGTTCA GGATATTTTG 
GGAGAACTTA GAGAAAAGGT GGGTGAGTGT GAAGCGTCTG CCATGCTGCC ACTGGAGTGC 
CAGTACTTGA ACAAAAACGC CCTGACGACC CTCGCGGGAC CCCTCACTCC CCCGGTGAAG 
CATTTTCAGT TAAAGCGGAA ACCCAAGAGC GCCACGCTGC GGGCGGAGCT GCTGCAGAAG 
TCCACGGAGA CCGCCCAGCA GTTGAAGCGG AGCGCCGGGG TGCCCTTCCA CGCCAAGGGC 
CGGGGGCTGC TGCGGAAGAT GGACACCACC ACCCCACTCA AAGGCATCCC GAAGCAGGCG 
CCCTTCAGAA GCCCCACGGC GCCCAGCGTC TTCAGCCCCA CAGGGAACCG GACCCCCATC 
CCGCCTTCCA GGACGCTGCT GCGGAAGGAA CGAGGTGTGA AGCTGCTGGA CATCTCTGAG 
CTGGATATGG TTGGCGCTGG CCGAGAGGCG AAGCGGAGAA GGAAGACTCT CGATGCGGAG 
GTGGTGGAGA AGCCGGCCAA GGAGGAAACG GTGGTGGAGA ACGCCACCCC GGACTACGCA 
GCCGGCCTGG TGTCCACGCA GAAACTTGGG TCCCTGAACA ATGAGCCTGC GCTGCCCTCC 
ACGAGCTACC TTCCCTCCAC GCCCAGCGTG GTTCCCGCCT CCTCCTACAT CCCCAGCTCC 
GAGACGCCCC CAGCCCCATC TTCCCGGGAA GCCAGCCGCC CACCAGAGGA GCCCAGCGCC 
CCGAGCCCCA CGTTGCCAGC GCAGTTCAAG CAGCGGGCGC CCATGTACAA CAGCGGCCTG 
AGCCCTGCCA CACCCACGCC TGCGGCGCCC ACCTCGCCTC TGACACCCAC CACACCTCCG 
GCTGTCGCCC CTACCACTCA GACACCCCCG GTTGCCATGG TGGCCCCGCA GACCCAGGCC 
CCTGCTCAGC AGCAGCCTAA GAAGAACCTG TCCCTCACGA GAGAGCAGAT GTTCGCTGCC 
CAGGAGATGT TCAAGACGGC CAACAAAGTC ACGCGGCCCG AGAAGGCCCT CATCCTGGGC 
TTCATGGCCG GCTCCCGAGA GAACCCGTGC CAGGAGCAGG GGGACGTGAT CCAGATCAAG 
CTGAGCGAGC ACACGGAGGA CCTGCCCAAG GCGGACGGCC AGGGTAGCAC AACCATGCTG 
GTGGACACAG TGTTTGAGAT GAACTATGCC ACGGGCCAGT GGACGCGCTT CAAGAAGTAC 
AAGCCCATGA CCAATGTGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864078.1
CDS	1586..3289
Translation	MPGQRRALSPKMASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDETPAVRSALCPFYSFLHAVLRHSNKWVCEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	XM_017008589.2
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member A (NELFA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008589.2

ORF Insert Sequence:

ATGCCGGGGC AGCGGCGCGC GCTTTCCCCC AAGATGGCGT CCATGCGGGA GAGCGACACG 
GGCCTGTGGC TGCACAACAA GCTGGGGGCC ACGGACGAGC TGTGGGCGCC GCCCAGCATC 
GCGTCCCTGC TCACGGCCGC GGTCATCGAC AACATCCGTC TCTGCTTCCA TGGCCTCTCG 
TCGGCAGTGA AGCTCAAGTT GCTACTCGGG ACGCTGCACC TCCCGCGCCG CACGGTGGAC 
GAGACCCCGG CCGTCCGGAG CGCTCTGTGT CCGTTTTACT CTTTTCTACA CGCCGTTTTG 
CGTCATTCCA ATAAGTGGGT GTGTGAGATG AAGGGCGCCC TAATGGAGAT CATCCAGCTC 
GCCAGCCTCG ACTCGGACCC CTGGGTGCTC ATGGTCGCCG ACATCTTGAA GTCCTTTCCG 
GACACAGGCT CGCTTAACCT GGAGCTGGAG GAGCAGAATC CCAACGTTCA GGATATTTTG 
GGAGAACTTA GAGAAAAGGT GGGTGAGTGT GAAGCGTCTG CCATGCTGCC ACTGGAGTGC 
CAGTACTTGA ACAAAAACGC CCTGACGACC CTCGCGGGAC CCCTCACTCC CCCGGTGAAG 
CATTTTCAGT TAAAGCGGAA ACCCAAGAGC GCCACGCTGC GGGCGGAGCT GCTGCAGAAG 
TCCACGGAGA CCGCCCAGCA GTTGAAGCGG AGCGCCGGGG TGCCCTTCCA CGCCAAGGGC 
CGGGGGCTGC TGCGGAAGAT GGACACCACC ACCCCACTCA AAGGCATCCC GAAGCAGGCG 
CCCTTCAGAA GCCCCACGGC GCCCAGCGTC TTCAGCCCCA CAGGGAACCG GACCCCCATC 
CCGCCTTCCA GGACGCTGCT GCGGAAGGAA CGAGGTGTGA AGCTGCTGGA CATCTCTGAG 
CTGGATATGG TTGGCGCTGG CCGAGAGGCG AAGCGGAGAA GGAAGACTCT CGATGCGGAG 
GTGGTGGAGA AGCCGGCCAA GGAGGAAACG GTGGTGGAGA ACGCCACCCC GGACTACGCA 
GCCGGCCTGG TGTCCACGCA GAAACTTGGG TCCCTGAACA ATGAGCCTGC GCTGCCCTCC 
ACGAGCTACC TTCCCTCCAC GCCCAGCGTG GTTCCCGCCT CCTCCTACAT CCCCAGCTCC 
GAGACGCCCC CAGCCCCATC TTCCCGGGAA GCCAGCCGCC CACCAGAGGA GCCCAGCGCC 
CCGAGCCCCA CGTTGCCAGC GCAGTTCAAG CAGCGGGCGC CCATGTACAA CAGCGGCCTG 
AGCCCTGCCA CACCCACGCC TGCGGCGCCC ACCTCGCCTC TGACACCCAC CACACCTCCG 
GCTGTCGCCC CTACCACTCA GACACCCCCG GTTGCCATGG TGGCCCCGCA GACCCAGGCC 
CCTGCTCAGC AGCAGCCTAA GAAGAACCTG TCCCTCACGA GAGAGCAGAT GTTCGCTGCC 
CAGGAGATGT TCAAGACGGC CAACAAAGTC ACGCGGCCCG AGAAGGCCCT CATCCTGGGC 
TTCATGGCCG GCTCCCGAGA GAACCCGTGC CAGGAGCAGG GGGACGTGAT CCAGATCAAG 
CTGAGCGAGC ACACGGAGGA CCTGCCCAAG GCGGACGGCC AGGGTAGCAC AACCATGCTG 
GTGGACACAG TGTTTGAGAT GAACTATGCC ACGGGCCAGT GGACGCGCTT CAAGAAGTAC 
AAGCCCATGA CCAATGTGTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFA ( NM_005663.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005654.4 Homo sapiens negative elongation factor complex member A (NELFA), mRNA.

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CloneID	OHu107765
Clone ID Related Accession (Same CDS sequence)	NM_005663.5
Accession Version	NM_005663.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	negative elongation factor A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002764.2 and AB044549.1. This sequence is a reference standard in the RefSeqGene project. On Nov 27, 2018 this sequence version replaced NM_005663.4. CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is supported by conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.510001.1, BC002764.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000382882.9/ ENSP00000372335.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCA TGCGGGAGAG CGACACGGGC CTGTGGCTGC ACAACAAGCT GGGGGCCACG 
GACGAGCTGT GGGCGCCGCC CAGCATCGCG TCCCTGCTCA CGGCCGCGGT CATCGACAAC 
ATCCGTCTCT GCTTCCATGG CCTCTCGTCG GCAGTGAAGC TCAAGTTGCT ACTCGGGACG 
CTGCACCTCC CGCGCCGCAC GGTGGACGAG ATGAAGGGCG CCCTAATGGA GATCATCCAG 
CTCGCCAGCC TCGACTCGGA CCCCTGGGTG CTCATGGTCG CCGACATCTT GAAGTCCTTT 
CCGGACACAG GCTCGCTTAA CCTGGAGCTG GAGGAGCAGA ATCCCAACGT TCAGGATATT 
TTGGGAGAAC TTAGAGAAAA GGTGGGTGAG TGTGAAGCGT CTGCCATGCT GCCACTGGAG 
TGCCAGTACT TGAACAAAAA CGCCCTGACG ACCCTCGCGG GACCCCTCAC TCCCCCGGTG 
AAGCATTTTC AGTTAAAGCG GAAACCCAAG AGCGCCACGC TGCGGGCGGA GCTGCTGCAG 
AAGTCCACGG AGACCGCCCA GCAGTTGAAG CGGAGCGCCG GGGTGCCCTT CCACGCCAAG 
GGCCGGGGGC TGCTGCGGAA GATGGACACC ACCACCCCAC TCAAAGGCAT CCCGAAGCAG 
GCGCCCTTCA GAAGCCCCAC GGCGCCCAGC GTCTTCAGCC CCACAGGGAA CCGGACCCCC 
ATCCCGCCTT CCAGGACGCT GCTGCGGAAG GAACGAGGTG TGAAGCTGCT GGACATCTCT 
GAGCTGGATA TGGTTGGCGC TGGCCGAGAG GCGAAGCGGA GAAGGAAGAC TCTCGATGCG 
GAGGTGGTGG AGAAGCCGGC CAAGGAGGAA ACGGTGGTGG AGAACGCCAC CCCGGACTAC 
GCAGCCGGCC TGGTGTCCAC GCAGAAACTT GGGTCCCTGA ACAATGAGCC TGCGCTGCCC 
TCCACGAGCT ACCTTCCCTC CACGCCCAGC GTGGTTCCCG CCTCCTCCTA CATCCCCAGC 
TCCGAGACGC CCCCAGCCCC ATCTTCCCGG GAAGCCAGCC GCCCACCAGA GGAGCCCAGC 
GCCCCGAGCC CCACGTTGCC AGCGCAGTTC AAGCAGCGGG CGCCCATGTA CAACAGCGGC 
CTGAGCCCTG CCACACCCAC GCCTGCGGCG CCCACCTCGC CTCTGACACC CACCACACCT 
CCGGCTGTCG CCCCTACCAC TCAGACACCC CCGGTTGCCA TGGTGGCCCC GCAGACCCAG 
GCCCCTGCTC AGCAGCAGCC TAAGAAGAAC CTGTCCCTCA CGAGAGAGCA GATGTTCGCT 
GCCCAGGAGA TGTTCAAGAC GGCCAACAAA GTCACGCGGC CCGAGAAGGC CCTCATCCTG 
GGCTTCATGG CCGGCTCCCG AGAGAACCCG TGCCAGGAGC AGGGGGACGT GATCCAGATC 
AAGCTGAGCG AGCACACGGA GGACCTGCCC AAGGCGGACG GCCAGGGTAG CACAACCATG 
CTGGTGGACA CAGTGTTTGA GATGAACTAT GCCACGGGCC AGTGGACGCG CTTCAAGAAG 
TACAAGCCCA TGACCAATGT GTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005654.4
CDS	16..1602
Translation	MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	NM_005663.5
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member A (NELFA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005663.5

ORF Insert Sequence:

ATGGCGTCCA TGCGGGAGAG CGACACGGGC CTGTGGCTGC ACAACAAGCT GGGGGCCACG 
GACGAGCTGT GGGCGCCGCC CAGCATCGCG TCCCTGCTCA CGGCCGCGGT CATCGACAAC 
ATCCGTCTCT GCTTCCATGG CCTCTCGTCG GCAGTGAAGC TCAAGTTGCT ACTCGGGACG 
CTGCACCTCC CGCGCCGCAC GGTGGACGAG ATGAAGGGCG CCCTAATGGA GATCATCCAG 
CTCGCCAGCC TCGACTCGGA CCCCTGGGTG CTCATGGTCG CCGACATCTT GAAGTCCTTT 
CCGGACACAG GCTCGCTTAA CCTGGAGCTG GAGGAGCAGA ATCCCAACGT TCAGGATATT 
TTGGGAGAAC TTAGAGAAAA GGTGGGTGAG TGTGAAGCGT CTGCCATGCT GCCACTGGAG 
TGCCAGTACT TGAACAAAAA CGCCCTGACG ACCCTCGCGG GACCCCTCAC TCCCCCGGTG 
AAGCATTTTC AGTTAAAGCG GAAACCCAAG AGCGCCACGC TGCGGGCGGA GCTGCTGCAG 
AAGTCCACGG AGACCGCCCA GCAGTTGAAG CGGAGCGCCG GGGTGCCCTT CCACGCCAAG 
GGCCGGGGGC TGCTGCGGAA GATGGACACC ACCACCCCAC TCAAAGGCAT CCCGAAGCAG 
GCGCCCTTCA GAAGCCCCAC GGCGCCCAGC GTCTTCAGCC CCACAGGGAA CCGGACCCCC 
ATCCCGCCTT CCAGGACGCT GCTGCGGAAG GAACGAGGTG TGAAGCTGCT GGACATCTCT 
GAGCTGGATA TGGTTGGCGC TGGCCGAGAG GCGAAGCGGA GAAGGAAGAC TCTCGATGCG 
GAGGTGGTGG AGAAGCCGGC CAAGGAGGAA ACGGTGGTGG AGAACGCCAC CCCGGACTAC 
GCAGCCGGCC TGGTGTCCAC GCAGAAACTT GGGTCCCTGA ACAATGAGCC TGCGCTGCCC 
TCCACGAGCT ACCTTCCCTC CACGCCCAGC GTGGTTCCCG CCTCCTCCTA CATCCCCAGC 
TCCGAGACGC CCCCAGCCCC ATCTTCCCGG GAAGCCAGCC GCCCACCAGA GGAGCCCAGC 
GCCCCGAGCC CCACGTTGCC AGCGCAGTTC AAGCAGCGGG CGCCCATGTA CAACAGCGGC 
CTGAGCCCTG CCACACCCAC GCCTGCGGCG CCCACCTCGC CTCTGACACC CACCACACCT 
CCGGCTGTCG CCCCTACCAC TCAGACACCC CCGGTTGCCA TGGTGGCCCC GCAGACCCAG 
GCCCCTGCTC AGCAGCAGCC TAAGAAGAAC CTGTCCCTCA CGAGAGAGCA GATGTTCGCT 
GCCCAGGAGA TGTTCAAGAC GGCCAACAAA GTCACGCGGC CCGAGAAGGC CCTCATCCTG 
GGCTTCATGG CCGGCTCCCG AGAGAACCCG TGCCAGGAGC AGGGGGACGT GATCCAGATC 
AAGCTGAGCG AGCACACGGA GGACCTGCCC AAGGCGGACG GCCAGGGTAG CACAACCATG 
CTGGTGGACA CAGTGTTTGA GATGAACTAT GCCACGGGCC AGTGGACGCG CTTCAAGAAG 
TACAAGCCCA TGACCAATGT GTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Molecular systems biology13(3)918(2017 03)
Sokolina K,Kittanakom S,Snider J,Kotlyar M,Maurice P,Gand?a J,Benleulmi-Chaachoua A,Tadagaki K,Oishi A,Wong V,Malty RH,Deineko V,Aoki H,Amin S,Yao Z,Morat? X,Otasek D,Kobayashi H,Menendez J,Auerbach D,Angers S,Pr?ulj N,Bouvier M,Babu M,Ciruela F,Jockers R,Jurisica I,Stagljar I

Architecture and RNA binding of the human negative elongation factor.
eLife5(2016 06)
Vos SM,P?llmann D,Caizzi L,Hofmann KB,Rombaut P,Zimniak T,Herzog F,Cramer P

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
The Journal of biological chemistry288(47)34055-72(2013 Nov)
Luo M,Lu X,Zhu R,Zhang Z,Chow CC,Li R,Simons SS Jr

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NELFA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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