Nelfa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfa
Entrez Gene ID	24116
Full Name	negative elongation factor complex member A, Whsc2
Synonyms	Whsc2,Whsc2h
General protein information	Preferred Names negative elongation factor complex member A, Whsc2 Names negative elongation factor A Wolf-Hirschhorn syndrome candidate 2 homolog mWHSC2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_011914.3	NP_036044.1	negative elongation factor A
XM_030254497.1	XP_030110357.1	negative elongation factor A isoform X1
NM_011914.2	NP_036044.1	negative elongation factor A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	nelfa	XP_002936807.1
Homo sapiens (human)	NELFA	NP_005654.3
Pan troglodytes (chimpanzee)	WHSC2	NP_001231577.1
Canis lupus familiaris (dog)	NELFA	XP_545924.2
Bos taurus (cattle)	WHSC2	NP_001095823.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002290	XP_001687824.2
	WHSC2	XP_001100646.2
Danio rerio (zebrafish)	nelfa	NP_001166972.1
Mus musculus (house mouse)	Nelfa	NP_036044.1
Rattus norvegicus (Norway rat)	Nelfa	NP_001008340.1
Gallus gallus (chicken)	WHSC2	NP_001026338.1

Gene Ontology
Bibliography

Related articles in PubMed

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Pan H, Zhao X, Zhang X, Abouelsoud M, Sun J, April C, Amleh A, Fan JB, Hu Y, Li R
PloS one10(5)e0127422(2015)

Chromatin loop organization of the junb locus in mouse dendritic cells.
Salem T, Gomard T, Court F, Moquet-Torcy G, Brockly F, Forn? T, Piechaczyk M
Nucleic acids research41(19)8908-25(2013 Oct)

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Flynn RA, Almada AE, Zamudio JR, Sharp PA
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS
Cell stem cell5(4)420-33(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nelfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu88905	XM_030254497.1 Latest version!	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu09153	NM_011914.3 Latest version!	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu09153	NM_011914.2	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfa ( XM_030254497.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110357.1 Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88905
Clone ID Related Accession (Same CDS sequence)	XM_030254497.1
Accession Version	XM_030254497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	negative elongation factor A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGG CTCTGATGGA TATCATCCAG CTGGCCACGC TGGACTCAGA CCCCTGGGTC 
CTCATGGTTG CTGACATTCT GAAGTCCTTC CCTGACACGG GCTCCTTGAA TCTGGACCTT 
GAGGAACAGA ACCCCAATGT TCAAGACATC TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG 
TGCGAGGCAT CTGCCATGCT ACCACTGGAG TGCCAGTACC TGAACAAGAA TGCCCTGACC 
ACCCTTGCTG GGCCCCTCAC CCCACCAGTC AAGCATTTTC AGCTAAAGCG GAAACCTAAG 
AGTGCCACGC TGCGGGCAGA ACTGCTGCAG AAATCTACCG AGACAGCCCA GCAGCTGAAG 
AGAAGTGCTG GGGTCCCATT CCACGCTAAG GGCCGGGGGC TGCTTCGAAA GATGGACACT 
ACAACCCCTC TCAAAGGCAT TCCGAAGCAA GCCCCCTTCC GAAGTCCCAC CACACCCAGC 
GTCTTCAGTC CTTCTGGGAA CCGGACTCCA ATACCACCTT CGAGGACGCC ACTGCAGAAA 
GAAAGGGGTG TGAAGCTGCT GGATATTTCT GAGCTGAATA CAGTTGGTGC TGGACGAGAG 
GCAAAGAGGA GGAGGAAGAC TTTAGACACA GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG 
ACGGTTGTTG AAAACGCTAC CCCTGACTAT GCTGCTGGCC TGGTATCCAC ACAGAAACTT 
GGGTCTCTGA ACAGTGAGCC AACACTGCCC TCCACAAGTT ACCTGCCCTC TACACCCAGT 
GTGGTACCTG CCTCATCTTA CATCCCCAGC TCTGAGACTC CCCCAGCTCC ACCCTCTCGG 
GAAGCCAGCC GGCCACCTGA GGAACCCAGC GCTCCAAGTC CCACACTGCC AACACAGTTT 
AAACAAAGGG CACCTATGTA TAACAGTGGC CTGAGCCCAG CTACACCAGC ACCTGCAGCA 
CCTACCTCAC CTCTGACACC TACTACTCCC CCAGCTGTCA CTCCTACTGC TCAGACACCT 
CCAGTGGCCA TGGTGGCCCC TCAGACCCAG GCTCCAGCCC CTGTTCAGCA GCAACCTAAA 
AAGAACCTGT CCCTCACTAG AGAACAGATG TTTGCTGCCC AGGAGATGTT CAAGACGGCA 
AACAAGGTCA CTCGGCCGGA GAAGGCCCTC ATCCTGGGCT TCATGGCTGG CTCTCGAGAG 
AACCCATGCC CGGAGCAGGG GGATGTGATC CAGATCAAGC TCAGTGAACA CACAGAGGAT 
CTGCCCAAGG CAGACGGCCA GGGCAGTACC ACCATGCTAG TGGACACAGT GTTCGAGATG 
AACTACGCCA CAGGCCAGTG GACACGCTTC AAGAAGTACA AGCCTATGAC CAATGTGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110357.1
CDS	342..1724
Translation	MKAALMDIIQLATLDSDPWVLMVADILKSFPDTGSLNLDLEEQNPNVQDILGELREKVSECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTTPSVFSPSGNRTPIPPSRTPLQKERGVKLLDISELNTVGAGREAKRRRKTLDTEVVEKPTKEETVVENATPDYAAGLVSTQKLGSLNSEPTLPSTSYLPSTPSVVPASSYIPSSETPPAPPSREASRPPEEPSAPSPTLPTQFKQRAPMYNSGLSPATPAPAAPTSPLTPTTPPAVTPTAQTPPVAMVAPQTQAPAPVQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCPEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	XM_030254497.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254497.1

ORF Insert Sequence:

ATGAAGGCGG CTCTGATGGA TATCATCCAG CTGGCCACGC TGGACTCAGA CCCCTGGGTC 
CTCATGGTTG CTGACATTCT GAAGTCCTTC CCTGACACGG GCTCCTTGAA TCTGGACCTT 
GAGGAACAGA ACCCCAATGT TCAAGACATC TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG 
TGCGAGGCAT CTGCCATGCT ACCACTGGAG TGCCAGTACC TGAACAAGAA TGCCCTGACC 
ACCCTTGCTG GGCCCCTCAC CCCACCAGTC AAGCATTTTC AGCTAAAGCG GAAACCTAAG 
AGTGCCACGC TGCGGGCAGA ACTGCTGCAG AAATCTACCG AGACAGCCCA GCAGCTGAAG 
AGAAGTGCTG GGGTCCCATT CCACGCTAAG GGCCGGGGGC TGCTTCGAAA GATGGACACT 
ACAACCCCTC TCAAAGGCAT TCCGAAGCAA GCCCCCTTCC GAAGTCCCAC CACACCCAGC 
GTCTTCAGTC CTTCTGGGAA CCGGACTCCA ATACCACCTT CGAGGACGCC ACTGCAGAAA 
GAAAGGGGTG TGAAGCTGCT GGATATTTCT GAGCTGAATA CAGTTGGTGC TGGACGAGAG 
GCAAAGAGGA GGAGGAAGAC TTTAGACACA GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG 
ACGGTTGTTG AAAACGCTAC CCCTGACTAT GCTGCTGGCC TGGTATCCAC ACAGAAACTT 
GGGTCTCTGA ACAGTGAGCC AACACTGCCC TCCACAAGTT ACCTGCCCTC TACACCCAGT 
GTGGTACCTG CCTCATCTTA CATCCCCAGC TCTGAGACTC CCCCAGCTCC ACCCTCTCGG 
GAAGCCAGCC GGCCACCTGA GGAACCCAGC GCTCCAAGTC CCACACTGCC AACACAGTTT 
AAACAAAGGG CACCTATGTA TAACAGTGGC CTGAGCCCAG CTACACCAGC ACCTGCAGCA 
CCTACCTCAC CTCTGACACC TACTACTCCC CCAGCTGTCA CTCCTACTGC TCAGACACCT 
CCAGTGGCCA TGGTGGCCCC TCAGACCCAG GCTCCAGCCC CTGTTCAGCA GCAACCTAAA 
AAGAACCTGT CCCTCACTAG AGAACAGATG TTTGCTGCCC AGGAGATGTT CAAGACGGCA 
AACAAGGTCA CTCGGCCGGA GAAGGCCCTC ATCCTGGGCT TCATGGCTGG CTCTCGAGAG 
AACCCATGCC CGGAGCAGGG GGATGTGATC CAGATCAAGC TCAGTGAACA CACAGAGGAT 
CTGCCCAAGG CAGACGGCCA GGGCAGTACC ACCATGCTAG TGGACACAGT GTTCGAGATG 
AACTACGCCA CAGGCCAGTG GACACGCTTC AAGAAGTACA AGCCTATGAC CAATGTGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfa ( NM_011914.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036044.1 Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.

Price & Availability↑

CloneID	OMu09153
Clone ID Related Accession (Same CDS sequence)	NM_011914.3 , NM_011914.2
Accession Version	NM_011914.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	negative elongation factor A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163329.6. On May 17, 2018 this sequence version replaced NM_011914.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK144173.1, AK019191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCCA TGAGGGAGAG TGACACCGGC CTGTGGCTGC ACAACAAGCT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCCGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCCTCG GCCGTGAAGC TCAAGCTACT GCTCGGGACG 
CTGCATCTCC CGCGCCGCAC CGTGGACGAG ATGAAGGCGG CTCTGATGGA TATCATCCAG 
CTGGCCACGC TGGACTCAGA CCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACGG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CTGCCATGCT ACCACTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GGCCCCTCAC CCCACCAGTC 
AAGCATTTTC AGCTAAAGCG GAAACCTAAG AGTGCCACGC TGCGGGCAGA ACTGCTGCAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGAAGTGCTG GGGTCCCATT CCACGCTAAG 
GGCCGGGGGC TGCTTCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT TCCGAAGCAA 
GCCCCCTTCC GAAGTCCCAC CACACCCAGC GTCTTCAGTC CTTCTGGGAA CCGGACTCCA 
ATACCACCTT CGAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGACGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAACGCTAC CCCTGACTAT 
GCTGCTGGCC TGGTATCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCTTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCTCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCTCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGCCCAG CTACACCAGC ACCTGCAGCA CCTACCTCAC CTCTGACACC TACTACTCCC 
CCAGCTGTCA CTCCTACTGC TCAGACACCT CCAGTGGCCA TGGTGGCCCC TCAGACCCAG 
GCTCCAGCCC CTGTTCAGCA GCAACCTAAA AAGAACCTGT CCCTCACTAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACGGCA AACAAGGTCA CTCGGCCGGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAG AACCCATGCC CGGAGCAGGG GGATGTGATC 
CAGATCAAGC TCAGTGAACA CACAGAGGAT CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACAGT GTTCGAGATG AACTACGCCA CAGGCCAGTG GACACGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036044.1
CDS	37..1629
Translation	MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHRLSSAVKLKLLLGTLHLPRRTVDEMKAALMDIIQLATLDSDPWVLMVADILKSFPDTGSLNLDLEEQNPNVQDILGELREKVSECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTTPSVFSPSGNRTPIPPSRTPLQKERGVKLLDISELNTVGAGREAKRRRKTLDTEVVEKPTKEETVVENATPDYAAGLVSTQKLGSLNSEPTLPSTSYLPSTPSVVPASSYIPSSETPPAPPSREASRPPEEPSAPSPTLPTQFKQRAPMYNSGLSPATPAPAAPTSPLTPTTPPAVTPTAQTPPVAMVAPQTQAPAPVQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCPEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	NM_011914.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011914.3

ORF Insert Sequence:

ATGGCTTCCA TGAGGGAGAG TGACACCGGC CTGTGGCTGC ACAACAAGCT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCCGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCCTCG GCCGTGAAGC TCAAGCTACT GCTCGGGACG 
CTGCATCTCC CGCGCCGCAC CGTGGACGAG ATGAAGGCGG CTCTGATGGA TATCATCCAG 
CTGGCCACGC TGGACTCAGA CCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACGG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CTGCCATGCT ACCACTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GGCCCCTCAC CCCACCAGTC 
AAGCATTTTC AGCTAAAGCG GAAACCTAAG AGTGCCACGC TGCGGGCAGA ACTGCTGCAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGAAGTGCTG GGGTCCCATT CCACGCTAAG 
GGCCGGGGGC TGCTTCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT TCCGAAGCAA 
GCCCCCTTCC GAAGTCCCAC CACACCCAGC GTCTTCAGTC CTTCTGGGAA CCGGACTCCA 
ATACCACCTT CGAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGACGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAACGCTAC CCCTGACTAT 
GCTGCTGGCC TGGTATCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCTTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCTCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCTCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGCCCAG CTACACCAGC ACCTGCAGCA CCTACCTCAC CTCTGACACC TACTACTCCC 
CCAGCTGTCA CTCCTACTGC TCAGACACCT CCAGTGGCCA TGGTGGCCCC TCAGACCCAG 
GCTCCAGCCC CTGTTCAGCA GCAACCTAAA AAGAACCTGT CCCTCACTAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACGGCA AACAAGGTCA CTCGGCCGGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAG AACCCATGCC CGGAGCAGGG GGATGTGATC 
CAGATCAAGC TCAGTGAACA CACAGAGGAT CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACAGT GTTCGAGATG AACTACGCCA CAGGCCAGTG GACACGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfa ( NM_011914.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036044.1 Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.

Price & Availability↑

CloneID	OMu09153
Clone ID Related Accession (Same CDS sequence)	NM_011914.3 , NM_011914.2
Accession Version	NM_011914.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	negative elongation factor A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC163329.6. On Nov 17, 2006 this sequence version replaced NM_011914.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038003.1, AK019191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCCA TGAGGGAGAG TGACACCGGC CTGTGGCTGC ACAACAAGCT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCCGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCCTCG GCCGTGAAGC TCAAGCTACT GCTCGGGACG 
CTGCATCTCC CGCGCCGCAC CGTGGACGAG ATGAAGGCGG CTCTGATGGA TATCATCCAG 
CTGGCCACGC TGGACTCAGA CCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACGG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CTGCCATGCT ACCACTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GGCCCCTCAC CCCACCAGTC 
AAGCATTTTC AGCTAAAGCG GAAACCTAAG AGTGCCACGC TGCGGGCAGA ACTGCTGCAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGAAGTGCTG GGGTCCCATT CCACGCTAAG 
GGCCGGGGGC TGCTTCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT TCCGAAGCAA 
GCCCCCTTCC GAAGTCCCAC CACACCCAGC GTCTTCAGTC CTTCTGGGAA CCGGACTCCA 
ATACCACCTT CGAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGACGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAACGCTAC CCCTGACTAT 
GCTGCTGGCC TGGTATCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCTTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCTCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCTCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGCCCAG CTACACCAGC ACCTGCAGCA CCTACCTCAC CTCTGACACC TACTACTCCC 
CCAGCTGTCA CTCCTACTGC TCAGACACCT CCAGTGGCCA TGGTGGCCCC TCAGACCCAG 
GCTCCAGCCC CTGTTCAGCA GCAACCTAAA AAGAACCTGT CCCTCACTAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACGGCA AACAAGGTCA CTCGGCCGGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAG AACCCATGCC CGGAGCAGGG GGATGTGATC 
CAGATCAAGC TCAGTGAACA CACAGAGGAT CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACAGT GTTCGAGATG AACTACGCCA CAGGCCAGTG GACACGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036044.1
CDS	24..1616
Translation	MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHRLSSAVKLKLLLGTLHLPRRTVDEMKAALMDIIQLATLDSDPWVLMVADILKSFPDTGSLNLDLEEQNPNVQDILGELREKVSECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTTPSVFSPSGNRTPIPPSRTPLQKERGVKLLDISELNTVGAGREAKRRRKTLDTEVVEKPTKEETVVENATPDYAAGLVSTQKLGSLNSEPTLPSTSYLPSTPSVVPASSYIPSSETPPAPPSREASRPPEEPSAPSPTLPTQFKQRAPMYNSGLSPATPAPAAPTSPLTPTTPPAVTPTAQTPPVAMVAPQTQAPAPVQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCPEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Target ORF information:

RefSeq Version	NM_011914.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member A, Whsc2 (Nelfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011914.2

ORF Insert Sequence:

ATGGCTTCCA TGAGGGAGAG TGACACCGGC CTGTGGCTGC ACAACAAGCT GGGCGCCACG 
GACGAGCTGT GGGCGCCACC CAGCATCGCG TCCCTGCTCA CCGCCGCGGT CATCGATAAC 
ATCCGCCTCT GCTTCCACCG CCTCTCCTCG GCCGTGAAGC TCAAGCTACT GCTCGGGACG 
CTGCATCTCC CGCGCCGCAC CGTGGACGAG ATGAAGGCGG CTCTGATGGA TATCATCCAG 
CTGGCCACGC TGGACTCAGA CCCCTGGGTC CTCATGGTTG CTGACATTCT GAAGTCCTTC 
CCTGACACGG GCTCCTTGAA TCTGGACCTT GAGGAACAGA ACCCCAATGT TCAAGACATC 
TTAGGAGAAC TTAGAGAAAA AGTGAGTGAG TGCGAGGCAT CTGCCATGCT ACCACTGGAG 
TGCCAGTACC TGAACAAGAA TGCCCTGACC ACCCTTGCTG GGCCCCTCAC CCCACCAGTC 
AAGCATTTTC AGCTAAAGCG GAAACCTAAG AGTGCCACGC TGCGGGCAGA ACTGCTGCAG 
AAATCTACCG AGACAGCCCA GCAGCTGAAG AGAAGTGCTG GGGTCCCATT CCACGCTAAG 
GGCCGGGGGC TGCTTCGAAA GATGGACACT ACAACCCCTC TCAAAGGCAT TCCGAAGCAA 
GCCCCCTTCC GAAGTCCCAC CACACCCAGC GTCTTCAGTC CTTCTGGGAA CCGGACTCCA 
ATACCACCTT CGAGGACGCC ACTGCAGAAA GAAAGGGGTG TGAAGCTGCT GGATATTTCT 
GAGCTGAATA CAGTTGGTGC TGGACGAGAG GCAAAGAGGA GGAGGAAGAC TTTAGACACA 
GAAGTGGTAG AAAAGCCCAC CAAGGAAGAG ACGGTTGTTG AAAACGCTAC CCCTGACTAT 
GCTGCTGGCC TGGTATCCAC ACAGAAACTT GGGTCTCTGA ACAGTGAGCC AACACTGCCC 
TCCACAAGTT ACCTGCCCTC TACACCCAGT GTGGTACCTG CCTCATCTTA CATCCCCAGC 
TCTGAGACTC CCCCAGCTCC ACCCTCTCGG GAAGCCAGCC GGCCACCTGA GGAACCCAGC 
GCTCCAAGTC CCACACTGCC AACACAGTTT AAACAAAGGG CACCTATGTA TAACAGTGGC 
CTGAGCCCAG CTACACCAGC ACCTGCAGCA CCTACCTCAC CTCTGACACC TACTACTCCC 
CCAGCTGTCA CTCCTACTGC TCAGACACCT CCAGTGGCCA TGGTGGCCCC TCAGACCCAG 
GCTCCAGCCC CTGTTCAGCA GCAACCTAAA AAGAACCTGT CCCTCACTAG AGAACAGATG 
TTTGCTGCCC AGGAGATGTT CAAGACGGCA AACAAGGTCA CTCGGCCGGA GAAGGCCCTC 
ATCCTGGGCT TCATGGCTGG CTCTCGAGAG AACCCATGCC CGGAGCAGGG GGATGTGATC 
CAGATCAAGC TCAGTGAACA CACAGAGGAT CTGCCCAAGG CAGACGGCCA GGGCAGTACC 
ACCATGCTAG TGGACACAGT GTTCGAGATG AACTACGCCA CAGGCCAGTG GACACGCTTC 
AAGAAGTACA AGCCTATGAC CAATGTGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
PloS one10(5)e0127422(2015)
Pan H,Zhao X,Zhang X,Abouelsoud M,Sun J,April C,Amleh A,Fan JB,Hu Y,Li R

Chromatin loop organization of the junb locus in mouse dendritic cells.
Nucleic acids research41(19)8908-25(2013 Oct)
Salem T,Gomard T,Court F,Moquet-Torcy G,Brockly F,Forn? T,Piechaczyk M

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)
Flynn RA,Almada AE,Zamudio JR,Sharp PA

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Cell stem cell5(4)420-33(2009 Oct)
Nishiyama A,Xin L,Sharov AA,Thomas M,Mowrer G,Meyers E,Piao Y,Mehta S,Yee S,Nakatake Y,Stagg C,Sharova L,Correa-Cerro LS,Bassey U,Hoang H,Kim E,Tapnio R,Qian Y,Dudekula D,Zalzman M,Li M,Falco G,Yang HT,Lee SL,Monti M,Stanghellini I,Islam MN,Nagaraja R,Goldberg I,Wang W,Longo DL,Schlessinger D,Ko MS

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Nelfa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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