ORF » Species Summary » Danio rerio » nelfa cDNA ORF clone

nelfa cDNA ORF clone, Danio rerio(zebrafish)

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  • Clones

  • gRNAs

The following nelfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nelfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32258 NM_001173501.1
Latest version!		 Danio rerio negative elongation factor complex member A (nelfa), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    nelfa ( NM_001173501.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001166972.1 Danio rerio negative elongation factor complex member A (nelfa), mRNA.
    Price & Availability↑
    CloneID ODa32258
    Clone ID Related Accession (Same CDS sequence) NM_001173501.1
    Accession Version NM_001173501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-26
    Organism Danio rerio(zebrafish)
    Product negative elongation factor A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD594623.1, CN018666.1, EB952822.1 and CR318672.5. On May 20, 2010 this sequence version replaced XM_001921215.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGTCGA TGAGGGAGAG CGACACCGGC CTGTGGCTCC ACAACAAACT CGGATCGACG 
    GACGAGCTGT GGACTCCTTC GAGCATCGCT TCCCTTCTCA CCGTGTCGGT CATCGATAAT 
    ATTCGGCTGT GTTTTTCCAG TCTTTCGCCA CCGGTGAAGC TGAAACTCTT GCTGGGGATG 
    CTGCACCTCC CGCGCAGGAC CGTGGATGAG ATGAAGGAGG CTCTGTCAGA GATCATTCAG 
    TTGGCCACAG TGGACTCTGA GCCCTGGGTG TTAATGGTGG CTGACATCCT GAAATCATTC 
    CCAGAGACCG GCTCACTAAA CCTGGACCTG GAGGAGCAGA ACCCTAATGT ACAGGACATC 
    CTTGGAGAAC TGCGTGAGAA AGTAAATGAA TGTGAAGCGT CAACCATGTT GCCTCTGGAG 
    TGTCAGTATC TGAACAAGAG TGCTTTGACC ACGCTGGTGG GGCCGCTCAC TCCACCCATC 
    AAACACTTCC AGCTCAAGAG GAAACCCAAG AGCGCCACAC TCCGAGCCGA GCTGCTGCAA 
    AAATCCACAG AGACCGCCCA ACAGTTAAAG AAAACCGCAG GAGTGCCCTT CCACTCCAAA 
    GGAAGAGGAC TAGTGAAGAA GATCGACACC ACAACGCCGC TGAAGGGAAT TCCCAAAGCA 
    CCATTCCGCA GCCCCACCAC CCCAAGCATG TTCAGCCCTC CCAGTAACAG AACCCCCATC 
    GCCCCTGCCC GCACACCCCT ACGTAAGGAG AGAGGTGTCA AGTTGTTGGA TATATCAGAG 
    TTGGATATGG TAGGAGCTGG TAGAGAAGCT AAGAGGAGAA GAAAGACTCT GGACACAGAA 
    GCTGGAGAAA AGGCAGCCAA AGAAGAGGCG GTGGTGGAAA ACGCCACACC AGACTACGCA 
    GCTGGCCTGG TTTCCACACA GAAACTTGGG GCGCTAAACA ATGAGAGTGC CCTGCCATCA 
    ACCAGTTACC TTCCCGCCAC TCCCAGCATG GTTCCTTCAT CGTCTTATAT TCCCAGCTCA 
    GAGACGCAGC CAGCGAACGC AGGTGGTTCA GGACGTGAAA CTACAAACCG ACAGTCGGAG 
    GAGTCGGCTG CCCCAAGTGC CGCTGCCGCT TCACTACCTG GCCAGTTCAA ACAGCGGCCG 
    CCTATGTATA ATGCCAGCAC TGCCAGTCCC ACCGCGCCCA CTTCACCCAC CACGCCAACC 
    AGCACGCCCA CAAACAATGG CCCGCCCCCT GCCGCCACAG CAGCACAGCC GGACATGCCC 
    ACACAGCCGC CCACCACTGC AGCTCAGCCT GCACCCACGC CAGCACCCCC ACAGACACAA 
    CCCAAAAAGA ACCTCTCGCT CACGAGGGAA CAGATGTATG CTGCACAAGA AATGTTCAAG 
    ACTGCAAACA AAGTCACACG GCCCGAAAAA GCCCTCATTC TGGGCTTCAT GGCTGGATCA 
    CGAGAAAACC CCTGTCCAGA GCAGGGTGAC ATCATTCAGA TCAAGCTGAG CGAGCACACA 
    GAAGTTCTGC CTAAAGCGGA CGGGACCGGC AGCACGACCA TGCTGGTGGA CACAGTGTTT 
    GAGATGAACT ACTCTACTGG GCAATGGACC CGCCTCAAGA AATACAAACC CATTACCAAC 
    GTCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001166972.1
    CDS72..1700
    Translation

    Target ORF information:

    RefSeq Version NM_001173501.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio negative elongation factor complex member A (nelfa), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001173501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGTCGA TGAGGGAGAG CGACACCGGC CTGTGGCTCC ACAACAAACT CGGATCGACG 
    GACGAGCTGT GGACTCCTTC GAGCATCGCT TCCCTTCTCA CCGTGTCGGT CATCGATAAT 
    ATTCGGCTGT GTTTTTCCAG TCTTTCGCCA CCGGTGAAGC TGAAACTCTT GCTGGGGATG 
    CTGCACCTCC CGCGCAGGAC CGTGGATGAG ATGAAGGAGG CTCTGTCAGA GATCATTCAG 
    TTGGCCACAG TGGACTCTGA GCCCTGGGTG TTAATGGTGG CTGACATCCT GAAATCATTC 
    CCAGAGACCG GCTCACTAAA CCTGGACCTG GAGGAGCAGA ACCCTAATGT ACAGGACATC 
    CTTGGAGAAC TGCGTGAGAA AGTAAATGAA TGTGAAGCGT CAACCATGTT GCCTCTGGAG 
    TGTCAGTATC TGAACAAGAG TGCTTTGACC ACGCTGGTGG GGCCGCTCAC TCCACCCATC 
    AAACACTTCC AGCTCAAGAG GAAACCCAAG AGCGCCACAC TCCGAGCCGA GCTGCTGCAA 
    AAATCCACAG AGACCGCCCA ACAGTTAAAG AAAACCGCAG GAGTGCCCTT CCACTCCAAA 
    GGAAGAGGAC TAGTGAAGAA GATCGACACC ACAACGCCGC TGAAGGGAAT TCCCAAAGCA 
    CCATTCCGCA GCCCCACCAC CCCAAGCATG TTCAGCCCTC CCAGTAACAG AACCCCCATC 
    GCCCCTGCCC GCACACCCCT ACGTAAGGAG AGAGGTGTCA AGTTGTTGGA TATATCAGAG 
    TTGGATATGG TAGGAGCTGG TAGAGAAGCT AAGAGGAGAA GAAAGACTCT GGACACAGAA 
    GCTGGAGAAA AGGCAGCCAA AGAAGAGGCG GTGGTGGAAA ACGCCACACC AGACTACGCA 
    GCTGGCCTGG TTTCCACACA GAAACTTGGG GCGCTAAACA ATGAGAGTGC CCTGCCATCA 
    ACCAGTTACC TTCCCGCCAC TCCCAGCATG GTTCCTTCAT CGTCTTATAT TCCCAGCTCA 
    GAGACGCAGC CAGCGAACGC AGGTGGTTCA GGACGTGAAA CTACAAACCG ACAGTCGGAG 
    GAGTCGGCTG CCCCAAGTGC CGCTGCCGCT TCACTACCTG GCCAGTTCAA ACAGCGGCCG 
    CCTATGTATA ATGCCAGCAC TGCCAGTCCC ACCGCGCCCA CTTCACCCAC CACGCCAACC 
    AGCACGCCCA CAAACAATGG CCCGCCCCCT GCCGCCACAG CAGCACAGCC GGACATGCCC 
    ACACAGCCGC CCACCACTGC AGCTCAGCCT GCACCCACGC CAGCACCCCC ACAGACACAA 
    CCCAAAAAGA ACCTCTCGCT CACGAGGGAA CAGATGTATG CTGCACAAGA AATGTTCAAG 
    ACTGCAAACA AAGTCACACG GCCCGAAAAA GCCCTCATTC TGGGCTTCAT GGCTGGATCA 
    CGAGAAAACC CCTGTCCAGA GCAGGGTGAC ATCATTCAGA TCAAGCTGAG CGAGCACACA 
    GAAGTTCTGC CTAAAGCGGA CGGGACCGGC AGCACGACCA TGCTGGTGGA CACAGTGTTT 
    GAGATGAACT ACTCTACTGG GCAATGGACC CGCCTCAAGA AATACAAACC CATTACCAAC 
    GTCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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