Bckdk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bckdk
Entrez Gene ID	29603
Full Name	branched chain ketoacid dehydrogenase kinase
General protein information	Preferred Names branched chain ketoacid dehydrogenase kinase Names [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial BCKD-kinase BCKDHKIN branched-chain alpha-ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q37
Summary	mitochondrial kinase; involved in oxidative degradation of branched-chain amino acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019244.2	NP_062117.2	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor

Pathways from BioSystems

Homologies

	BCKDK	XP_001112186.1
Danio rerio (zebrafish)	bckdk	NP_998225.1
Saccharomyces cerevisiae (baker's yeast)	PKP1	NP_012222.1
Homo sapiens (human)	BCKDK	NP_005872.2
Kluyveromyces lactis	KLLA0E04533g	XP_454150.1
Magnaporthe oryzae (rice blast fungus)	MGG_06200	XP_003712087.1
Mus musculus (house mouse)	Bckdk	NP_033869.1
Rattus norvegicus (Norway rat)	Bckdk	NP_062117.2
Canis lupus familiaris (dog)	BCKDK	XP_536903.2
Bos taurus (cattle)	BCKDK	NP_001039371.1
Xenopus tropicalis (tropical clawed frog)	bckdk	XP_004919384.1
Pan troglodytes (chimpanzee)	BCKDK	XP_001156688.1
Eremothecium gossypii	AGOS_ADL116C	NP_983980.1
Neurospora crassa	NCU04349	XP_956330.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Tissue-specific translation of murine branched-chain alpha-ketoacid dehydrogenase kinase mRNA is dependent upon an upstream open reading frame in the 5'-untranslated region.
Muller EA, Danner DJ
The Journal of biological chemistry279(43)44645-55(2004 Oct)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Structure of rat BCKD kinase: nucleotide-induced domain communication in a mitochondrial protein kinase.
Machius M, Chuang JL, Wynn RM, Tomchick DR, Chuang DT
Proceedings of the National Academy of Sciences of the United States of America98(20)11218-23(2001 Sep)

Murine branched chain alpha-ketoacid dehydrogenase kinase; cDNA cloning, tissue distribution, and temporal expression during embryonic development.
Doering CB, Coursey C, Spangler W, Danner DJ
Gene212(2)213-9(1998 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bckdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bckdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12049	NM_019244.2 Latest version!	Rattus norvegicus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bckdk ( NM_019244.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062117.2 Rattus norvegicus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.

Price & Availability↑

CloneID	ORa12049
Clone ID Related Accession (Same CDS sequence)	NM_019244.2
Accession Version	NM_019244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK479351.1, U27456.1 and BM392152.1. On Oct 20, 2007 this sequence version replaced NM_019244.1. ##Evidence-Data-START## CDS exon combination :: M93271.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATACTGA CTTCAGTGCT GGGCAGCGGC CCTCGGAGCG GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGTTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTT ACCTCCTTTT ACAACCAGTC AGCTATTGAC 
GTGGTAGCAG AGAAGCCCTC AGTCCGCCTC ACTCCCACCA TGATGCTCTA TTCTGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGTCGCTACT TGCAGCAAGA GTTACCGGTG 
AGGATCGCTC ACCGCATCAA GGGCTTCCGT AGTCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGGGCCTTCC AGAAGTTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA CGAAGCCCAG TATTGCCAGC TGGTGCGACA GCTGCTAGAT 
GACCACAAGG ATGTGGTAAC CCTGTTAGCT GAGGGTCTGC GTGAGAGCCG GAAACACATA 
GAGGATGAAA AGCTGGTCCG CTACTTCCTG GATAAAACAC TAACGTCCAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGCACTC GTCTGTCACC CAAGAAGATT ATTGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGCGAGC ACAAGTATGG CAATGCCCCT AGAGTCCGCA TCAATGGGCA CGTGGCTGCC 
CGTTTCCCCT TCATTCCTAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAACGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACGCCCTACA ATGTTCCTGA TGTGGTCATC 
ACCATCGCCA ATAACGATGT GGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAGCCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAATCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCCTATGC GGAGTATCTC 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGCCGGGA GGAAAGCTTC AGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062117.2
CDS	257..1495
Translation	MILTSVLGSGPRSGSSLWPLLGSSLSLRVRSTSATDTHHVELARERSKTVTSFYNQSAIDVVAEKPSVRLTPTMMLYSGRSQDGSHLLKSGRYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGGQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Target ORF information:

RefSeq Version	NM_019244.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019244.2

ORF Insert Sequence:

ATGATACTGA CTTCAGTGCT GGGCAGCGGC CCTCGGAGCG GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGTTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTT ACCTCCTTTT ACAACCAGTC AGCTATTGAC 
GTGGTAGCAG AGAAGCCCTC AGTCCGCCTC ACTCCCACCA TGATGCTCTA TTCTGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGTCGCTACT TGCAGCAAGA GTTACCGGTG 
AGGATCGCTC ACCGCATCAA GGGCTTCCGT AGTCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGGGCCTTCC AGAAGTTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA CGAAGCCCAG TATTGCCAGC TGGTGCGACA GCTGCTAGAT 
GACCACAAGG ATGTGGTAAC CCTGTTAGCT GAGGGTCTGC GTGAGAGCCG GAAACACATA 
GAGGATGAAA AGCTGGTCCG CTACTTCCTG GATAAAACAC TAACGTCCAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGCACTC GTCTGTCACC CAAGAAGATT ATTGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGCGAGC ACAAGTATGG CAATGCCCCT AGAGTCCGCA TCAATGGGCA CGTGGCTGCC 
CGTTTCCCCT TCATTCCTAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAACGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACGCCCTACA ATGTTCCTGA TGTGGTCATC 
ACCATCGCCA ATAACGATGT GGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAGCCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAATCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCCTATGC GGAGTATCTC 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGCCGGGA GGAAAGCTTC AGAATCTGA