BCKDK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCKDK
Entrez Gene ID	10295
Full Name	branched chain ketoacid dehydrogenase kinase
Synonyms	BCKDKD,BDK
General protein information	Preferred Names branched chain ketoacid dehydrogenase kinase Names [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial 3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial BCKD-kinase BCKDHKIN branched chain alpha-ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012].
Disorder MIM:	614901
Disorder Html:	Branched-chain ketoacid dehydrogenase kinase deficiency, 614923 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122957.2	NP_001116429.1	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform b precursor
NM_001271926.1	NP_001258855.1	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform c
NM_005881.4	NP_005872.2	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform a precursor
NM_001122957.3	NP_001116429.1	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform b precursor
NM_005881.3	NP_005872.2	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform a precursor
XM_017022859.1	XP_016878348.1	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial isoform X1
NM_001271926.2	NP_001258855.1	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform c

Pathways from BioSystems

Homologies

	BCKDK	XP_001112186.1
Danio rerio (zebrafish)	bckdk	NP_998225.1
Saccharomyces cerevisiae (baker's yeast)	PKP1	NP_012222.1
Homo sapiens (human)	BCKDK	NP_005872.2
Kluyveromyces lactis	KLLA0E04533g	XP_454150.1
Magnaporthe oryzae (rice blast fungus)	MGG_06200	XP_003712087.1
Mus musculus (house mouse)	Bckdk	NP_033869.1
Rattus norvegicus (Norway rat)	Bckdk	NP_062117.2
Canis lupus familiaris (dog)	BCKDK	XP_536903.2
Bos taurus (cattle)	BCKDK	NP_001039371.1
Xenopus tropicalis (tropical clawed frog)	bckdk	XP_004919384.1
Pan troglodytes (chimpanzee)	BCKDK	XP_001156688.1
Eremothecium gossypii	AGOS_ADL116C	NP_983980.1
Neurospora crassa	NCU04349	XP_956330.1

Gene Ontology
Bibliography

Related articles in PubMed

BCKDK of BCAA Catabolism Cross-talking With the MAPK Pathway Promotes Tumorigenesis of Colorectal Cancer.
Xue P, Zeng F, Duan Q, Xiao J, Liu L, Yuan P, Fan L, Sun H, Malyarenko OS, Lu H, Xiu R, Liu S, Shao C, Zhang J, Yan W, Wang Z, Zheng J, Zhu F
EBioMedicine2050-60(2017 Jun)

The plasma levels of CST and BCKDK in patients with sepsis.
Zhang B, Sun GZ, Zhu ML, Li Y, Sun DJ, Zhang B, Bai XP
Peptides8680-84(2016 Dec)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Large-scale meta-analysis of genome-wide association data identifies six new risk loci for Parkinson's disease.
Nalls MA, Pankratz N, Lill CM, Do CB, Hernandez DG, Saad M, DeStefano AL, Kara E, Bras J, Sharma M, Schulte C, Keller MF, Arepalli S, Letson C, Edsall C, Stefansson H, Liu X, Pliner H, Lee JH, Cheng R, , , , , , , , , , , , , Ikram MA, Ioannidis JP, Hadjigeorgiou GM, Bis JC, Martinez M, Perlmutter JS, Goate A, Marder K, Fiske B, Sutherland M, Xiromerisiou G, Myers RH, Clark LN, Stefansson K, Hardy JA, Heutink P, Chen H, Wood NW, Houlden H, Payami H, Brice A, Scott WK, Gasser T, Bertram L, Eriksson N, Foroud T, Singleton AB
Nature genetics46(9)989-93(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There is a relationship between plamsa CST and BCKDK in sepsis patients.
Title: The plasma levels of CST and BCKDK in patients with sepsis.

Results demonstrate that BCKDK mutations can result in neurobehavioral deficits in humans.
Title: Two novel mutations in the BCKDK (branched-chain keto-acid dehydrogenase kinase) gene are responsible for a neurobehavioral deficit in two pediatric unrelated patients.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following BCKDK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14188	NM_001122957.3 Latest version!	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu14243	NM_001271926.2 Latest version!	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20674	NM_005881.4 Latest version!	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu85810	XM_017022859.1 Latest version!	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu14188	NM_001122957.2	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu20674	NM_005881.3	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14243	NM_001271926.1	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCKDK ( NM_001122957.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116429.1 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14188
Clone ID Related Accession (Same CDS sequence)	NM_001122957.2 , NM_001122957.3
Accession Version	NM_001122957.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, AC135050.3, BC009872.2 and BM679327.1. On Dec 12, 2012 this sequence version replaced NM_001122957.1. Transcript Variant: This variant (2) retains an intron in the 3' end of the coding sequence compared to variant 1. The resulting isoform (b) is shorter at the C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.244445.1, SRR1163658.389444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116429.1
CDS	364..1461
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHG

Target ORF information:

RefSeq Version	NM_001122957.2
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122957.2

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( NM_001122957.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116429.1 Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14188
Clone ID Related Accession (Same CDS sequence)	NM_001122957.2 , NM_001122957.3
Accession Version	NM_001122957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, AC135050.3, BC009872.2 and BM679327.1. On May 31, 2019 this sequence version replaced NM_001122957.2. Transcript Variant: This variant (2) retains an intron in the 3' end of the coding sequence compared to variant 1. The resulting isoform (b) is shorter at the C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1124567.1, SRR1803614.244445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116429.1
CDS	272..1369
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHG

Target ORF information:

RefSeq Version	NM_001122957.3
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122957.3

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( NM_001271926.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258855.1 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14243
Clone ID Related Accession (Same CDS sequence)	NM_001271926.1 , NM_001271926.2
Accession Version	NM_001271926.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, AC135050.3, BC009872.2 and BM679327.1. Transcript Variant: This variant (3) retains an alternate exon in the 3' coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) is shorter at the C-terminus and lacks an alternate internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009872.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGGATCT CAGACCGTGG TGGAGGAATC GCTCACAAAG ATCTGGACCG GGTCATGGAC 
TACCACTTCA CTACTGCTGA GGCCAGCACA CAGGACCCCC GGATCAGCCC CCTCTTTGGC 
CATCTGGACA TGCATAGTGG CGCCCAGTCA GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258855.1
CDS	364..1371
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHG

Target ORF information:

RefSeq Version	NM_001271926.1
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271926.1

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGGATCT CAGACCGTGG TGGAGGAATC GCTCACAAAG ATCTGGACCG GGTCATGGAC 
TACCACTTCA CTACTGCTGA GGCCAGCACA CAGGACCCCC GGATCAGCCC CCTCTTTGGC 
CATCTGGACA TGCATAGTGG CGCCCAGTCA GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( NM_001271926.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258855.1 Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.

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CloneID	OHu14243
Clone ID Related Accession (Same CDS sequence)	NM_001271926.1 , NM_001271926.2
Accession Version	NM_001271926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, BC009872.2 and BM679327.1. On May 31, 2019 this sequence version replaced NM_001271926.1. Transcript Variant: This variant (3) retains an alternate exon in the 3' coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) is shorter at the C-terminus and lacks an alternate internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009872.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGGATCT CAGACCGTGG TGGAGGAATC GCTCACAAAG ATCTGGACCG GGTCATGGAC 
TACCACTTCA CTACTGCTGA GGCCAGCACA CAGGACCCCC GGATCAGCCC CCTCTTTGGC 
CATCTGGACA TGCATAGTGG CGCCCAGTCA GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258855.1
CDS	272..1279
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHG

Target ORF information:

RefSeq Version	NM_001271926.2
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271926.2

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGGATCT CAGACCGTGG TGGAGGAATC GCTCACAAAG ATCTGGACCG GGTCATGGAC 
TACCACTTCA CTACTGCTGA GGCCAGCACA CAGGACCCCC GGATCAGCCC CCTCTTTGGC 
CATCTGGACA TGCATAGTGG CGCCCAGTCA GGACCCATGC ACGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( NM_005881.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005872.2 Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.

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CloneID	OHu20674
Clone ID Related Accession (Same CDS sequence)	NM_005881.4 , NM_005881.3
Accession Version	NM_005881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, AC135050.3, BC007363.2 and BM679327.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005881.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.180227.1, SRR1803615.178557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000219794.11/ ENSP00000219794.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGCTTTGG CTTCGGGTTG CCCACGTCAC GGGCCTACGC GGAGTACCTC 
GGTGGGTCTC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGACGTCTA CCTGCGGCTC 
CGCCACATCG ATGGCCGGGA GGAAAGCTTC CGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005872.2
CDS	272..1510
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Target ORF information:

RefSeq Version	NM_005881.4
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005881.4

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGCTTTGG CTTCGGGTTG CCCACGTCAC GGGCCTACGC GGAGTACCTC 
GGTGGGTCTC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGACGTCTA CCTGCGGCTC 
CGCCACATCG ATGGCCGGGA GGAAAGCTTC CGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( NM_005881.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005872.2 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.

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CloneID	OHu20674
Clone ID Related Accession (Same CDS sequence)	NM_005881.4 , NM_005881.3
Accession Version	NM_005881.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX427330.2, AC135050.3, BC007363.2 and BM679327.1. This sequence is a reference standard in the RefSeqGene project. On Dec 12, 2012 this sequence version replaced NM_005881.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.180227.1, SRR1803615.178557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGCTTTGG CTTCGGGTTG CCCACGTCAC GGGCCTACGC GGAGTACCTC 
GGTGGGTCTC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGACGTCTA CCTGCGGCTC 
CGCCACATCG ATGGCCGGGA GGAAAGCTTC CGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005872.2
CDS	364..1602
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Target ORF information:

RefSeq Version	NM_005881.3
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005881.3

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGCTTTGG CTTCGGGTTG CCCACGTCAC GGGCCTACGC GGAGTACCTC 
GGTGGGTCTC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGACGTCTA CCTGCGGCTC 
CGCCACATCG ATGGCCGGGA GGAAAGCTTC CGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDK ( XM_017022859.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878348.1 Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant X1, mRNA.

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CloneID	OHu85810
Clone ID Related Accession (Same CDS sequence)	XM_017022859.1
Accession Version	XM_017022859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGCTGCA GCCACAGGTC CGCACGGAAA ACCAGGAGCT CCAAGACTGC 
CGCACACTCA ACATCCAACT CGCGGGGCCG CGGGGACGGC CACAGAAGCC AATCGGCTCC 
TGCTACATCA CAGACCCCGC CCTCAAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878348.1
CDS	277..1509
Translation	MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGLQPQVRTENQELQDCRTLNIQLAGPRGRPQKPIGSCYITDPALKP

Target ORF information:

RefSeq Version	XM_017022859.1
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022859.1

ORF Insert Sequence:

ATGATCCTGG CGTCGGTGCT GAGGAGCGGT CCCGGGGGCG GGCTTCCGCT CCGGCCCCTC 
CTGGGACCCG CACTCGCGCT CCGGGCCCGC TCGACGTCGG CCACCGACAC ACACCACGTG 
GAGATGGCTC GGGAGCGCTC CAAGACCGTC ACCTCCTTTT ACAACCAGTC GGCCATCGAC 
GCGGCAGCGG AGAAGCCCTC AGTCCGCCTA ACGCCCACCA TGATGCTCTA CGCTGGCCGC 
TCTCAGGACG GCAGCCACCT TCTGAAAAGT GCTCGGTACC TGCAGCAAGA ACTTCCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGC TGCCTTCCTT TCATCATTGG CTGCAACCCC 
ACCATACTGC ACGTGCATGA GCTATATATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCGGA CGAGGCCCAG TACTGCCAGC TGGTGCGACA GCTGCTGGAT 
GACCACAAGG ATGTGGTGAC CCTCTTGGCA GAGGGCCTAC GTGAGAGCCG GAAGCACATA 
GAGGATGAAA AGCTCGTCCG CTACTTCTTG GACAAGACGC TGACTTCGAG GCTTGGAATC 
CGCATGTTGG CCACGCATCA CCTGGCGCTG CATGAGGACA AGCCTGACTT TGTCGGCATC 
ATCTGTACTC GTCTCTCACC AAAGAAGATT ATTGAGAAGT GGGTGGACTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCGCCC CGTGTCCGCA TCAATGGCCA TGTGGCTGCC 
CGGTTCCCCT TCATCCCTAT GCCACTGGAC TACATCCTGC CGGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ACAATGATGT CGATCTGATC ATCAGGATCT CAGACCGTGG TGGAGGAATC 
GCTCACAAAG ATCTGGACCG GGTCATGGAC TACCACTTCA CTACTGCTGA GGCCAGCACA 
CAGGACCCCC GGATCAGCCC CCTCTTTGGC CATCTGGACA TGCATAGTGG CGCCCAGTCA 
GGACCCATGC ACGGGCTGCA GCCACAGGTC CGCACGGAAA ACCAGGAGCT CCAAGACTGC 
CGCACACTCA ACATCCAACT CGCGGGGCCG CGGGGACGGC CACAGAAGCC AATCGGCTCC 
TGCTACATCA CAGACCCCGC CCTCAAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BCKDK of BCAA Catabolism Cross-talking With the MAPK Pathway Promotes Tumorigenesis of Colorectal Cancer.
EBioMedicine2050-60(2017 Jun)
Xue P,Zeng F,Duan Q,Xiao J,Liu L,Yuan P,Fan L,Sun H,Malyarenko OS,Lu H,Xiu R,Liu S,Shao C,Zhang J,Yan W,Wang Z,Zheng J,Zhu F

The plasma levels of CST and BCKDK in patients with sepsis.
Peptides8680-84(2016 Dec)
Zhang B,Sun GZ,Zhu ML,Li Y,Sun DJ,Zhang B,Bai XP

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Large-scale meta-analysis of genome-wide association data identifies six new risk loci for Parkinson's disease.
Nature genetics46(9)989-93(2014 Sep)
Nalls MA,Pankratz N,Lill CM,Do CB,Hernandez DG,Saad M,DeStefano AL,Kara E,Bras J,Sharma M,Schulte C,Keller MF,Arepalli S,Letson C,Edsall C,Stefansson H,Liu X,Pliner H,Lee JH,Cheng R,,,,,,,,,,,,,Ikram MA,Ioannidis JP,Hadjigeorgiou GM,Bis JC,Martinez M,Perlmutter JS,Goate A,Marder K,Fiske B,Sutherland M,Xiromerisiou G,Myers RH,Clark LN,Stefansson K,Hardy JA,Heutink P,Chen H,Wood NW,Houlden H,Payami H,Brice A,Scott WK,Gasser T,Bertram L,Eriksson N,Foroud T,Singleton AB

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BCKDK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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