Bckdk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bckdk
Entrez Gene ID	12041
Full Name	branched chain ketoacid dehydrogenase kinase
Synonyms	AI327402
General protein information	Preferred Names branched chain ketoacid dehydrogenase kinase Names [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial BCKD-kinase BCKDHKIN branched-chain alpha-ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_009739.3	NP_033869.1	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor

Pathways from BioSystems

Homologies

	BCKDK	XP_001112186.1
Danio rerio (zebrafish)	bckdk	NP_998225.1
Saccharomyces cerevisiae (baker's yeast)	PKP1	NP_012222.1
Homo sapiens (human)	BCKDK	NP_005872.2
Kluyveromyces lactis	KLLA0E04533g	XP_454150.1
Magnaporthe oryzae (rice blast fungus)	MGG_06200	XP_003712087.1
Mus musculus (house mouse)	Bckdk	NP_033869.1
Rattus norvegicus (Norway rat)	Bckdk	NP_062117.2
Canis lupus familiaris (dog)	BCKDK	XP_536903.2
Bos taurus (cattle)	BCKDK	NP_001039371.1
Xenopus tropicalis (tropical clawed frog)	bckdk	XP_004919384.1
Pan troglodytes (chimpanzee)	BCKDK	XP_001156688.1
Eremothecium gossypii	AGOS_ADL116C	NP_983980.1
Neurospora crassa	NCU04349	XP_956330.1

Gene Ontology
Bibliography

Related articles in PubMed

Endurance performance and energy metabolism during exercise in mice with a muscle-specific defect in the control of branched-chain amino acid catabolism.
Xu M, Kitaura Y, Ishikawa T, Kadota Y, Terai C, Shindo D, Morioka T, Ota M, Morishita Y, Ishihara K, Shimomura Y
PloS one12(7)e0180989(2017)

Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.
Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y
Scientific reports739825(2017 Jan)

Impaired Amino Acid Transport at the Blood Brain Barrier Is a Cause of Autism Spectrum Disorder.
T?rlungeanu DC, Deliu E, Dotter CP, Kara M, Janiesch PC, Scalise M, Galluccio M, Tesulov M, Morelli E, Sonmez FM, Bilguvar K, Ohgaki R, Kanai Y, Johansen A, Esharif S, Ben-Omran T, Topcu M, Schlessinger A, Indiveri C, Duncan KE, Caglayan AO, Gunel M, Gleeson JG, Novarino G
Cell167(6)1481-1494.e18(2016 Dec)

A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent.
Cao H, Yu W, Li X, Wang J, Gao S, Holton NE, Eliason S, Sharp T, Amendt BA
Gene therapy23(6)527-42(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that targeting mitochondrial BCKD kinase (BDK) as a pharmacological approach to mitigate the branched-chain amino acids (BCAAs) accumulation in metabolic diseases and heart failure.
Title: Structure-based design and mechanisms of allosteric inhibitors for mitochondrial branched-chain ?-ketoacid dehydrogenase kinase.

leucine supplementation increased the expression of enzymes (BCAT1, BCAT2 and BCKDK) that metabolize branched-chain amino acids.
Title: Oral leucine supplementation is sensed by the brain but neither reduces food intake nor induces an anorectic pattern of gene expression in the hypothalamus.

branched-chain a-keto acid dehydrogenase complex kinase plays an important role in conserving branched-chain amino acids by limiting their oxidation
Title: Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice.

Tissue-specific translation of murine BCKDK mRNA is dependent upon an upstream open reading frame in the 5'-untranslated region
Title: Tissue-specific translation of murine branched-chain alpha-ketoacid dehydrogenase kinase mRNA is dependent upon an upstream open reading frame in the 5'-untranslated region.

The following Bckdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bckdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13050	NM_009739.3 Latest version!	Mus musculus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bckdk ( NM_009739.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033869.1 Mus musculus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.

Price & Availability↑

CloneID	OMu13050
Clone ID Related Accession (Same CDS sequence)	NM_009739.3
Accession Version	NM_009739.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY077717.1 and AK150607.1. On Feb 25, 2006 this sequence version replaced NM_009739.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150607.1, AK167519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 15302860 ##RefSeq-Attributes-END##

ATGATACTGA CTTCAGTGCT GGGCAGCGGC CCTCGGAGCT GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGCTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTC ACCTCTTTTT ACAACCAGTC AGCCATTGAT 
GTGGCAGCAG AGAAGCCCTC AGTTCGCCTC ACTCCCACCA TGATGCTCTA TTCCGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGCCGCTACT TGCAGCAAGA GTTACCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGT AGCCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGGGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA TGAGGCCCAG TACTGCCAGC TGGTGAGACA GCTGCTAGAT 
GACCACAAGG ATGTGGTGAC CCTGTTAGCT GAGGGTCTTC GTGAGAGCCG GAAACACATC 
CAGGATGAAA AGCTAGTCCG GTACTTCTTG GATAAAACAC TGACGTCAAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGTACTC GTCTGTCGCC CAAGAAGATT ATCGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCCCCT CGAGTCCGCA TCAACGGACA TGTGGCTGCC 
CGGTTCCCCT TCATTCCCAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ATAATGATAT TGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAACCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAGTCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCATATGC AGAGTATCTT 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGTCGGGA GGAAAGCTTC AGAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033869.1
CDS	266..1504
Translation	MILTSVLGSGPRSWSSLWPLLGSSLSLRARSTSATDTHHVELARERSKTVTSFYNQSAIDVAAEKPSVRLTPTMMLYSGRSQDGSHLLKSGRYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDIDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRINPLFGHLDMHSGGQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Target ORF information:

RefSeq Version	NM_009739.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus branched chain ketoacid dehydrogenase kinase (Bckdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009739.3

ORF Insert Sequence:

ATGATACTGA CTTCAGTGCT GGGCAGCGGC CCTCGGAGCT GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGCTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTC ACCTCTTTTT ACAACCAGTC AGCCATTGAT 
GTGGCAGCAG AGAAGCCCTC AGTTCGCCTC ACTCCCACCA TGATGCTCTA TTCCGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGCCGCTACT TGCAGCAAGA GTTACCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGT AGCCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGGGCCTTCC AGAAGCTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA TGAGGCCCAG TACTGCCAGC TGGTGAGACA GCTGCTAGAT 
GACCACAAGG ATGTGGTGAC CCTGTTAGCT GAGGGTCTTC GTGAGAGCCG GAAACACATC 
CAGGATGAAA AGCTAGTCCG GTACTTCTTG GATAAAACAC TGACGTCAAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGTACTC GTCTGTCGCC CAAGAAGATT ATCGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCCCCT CGAGTCCGCA TCAACGGACA TGTGGCTGCC 
CGGTTCCCCT TCATTCCCAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAATGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ATAATGATAT TGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAACCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAGTCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCATATGC AGAGTATCTT 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CGGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGTCGGGA GGAAAGCTTC AGAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endurance performance and energy metabolism during exercise in mice with a muscle-specific defect in the control of branched-chain amino acid catabolism.
PloS one12(7)e0180989(2017)
Xu M,Kitaura Y,Ishikawa T,Kadota Y,Terai C,Shindo D,Morioka T,Ota M,Morishita Y,Ishihara K,Shimomura Y

Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.
Scientific reports739825(2017 Jan)
Ishikawa T,Kitaura Y,Kadota Y,Morishita Y,Ota M,Yamanaka F,Xu M,Ikawa M,Inoue N,Kawano F,Nakai N,Murakami T,Miura S,Hatazawa Y,Kamei Y,Shimomura Y

Impaired Amino Acid Transport at the Blood Brain Barrier Is a Cause of Autism Spectrum Disorder.
Cell167(6)1481-1494.e18(2016 Dec)
T?rlungeanu DC,Deliu E,Dotter CP,Kara M,Janiesch PC,Scalise M,Galluccio M,Tesulov M,Morelli E,Sonmez FM,Bilguvar K,Ohgaki R,Kanai Y,Johansen A,Esharif S,Ben-Omran T,Topcu M,Schlessinger A,Indiveri C,Duncan KE,Caglayan AO,Gunel M,Gleeson JG,Novarino G

A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent.
Gene therapy23(6)527-42(2016 06)
Cao H,Yu W,Li X,Wang J,Gao S,Holton NE,Eliason S,Sharp T,Amendt BA