The following Dgcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa11568 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006248606.3 , NM_001105865.1 , XM_008768865.2 | |
| Accession Version | NM_001105865.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-07 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | microprocessor complex subunit DGCR8 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473999.2. On or before Oct 3, 2007 this sequence version replaced XM_221273.4, XM_001064513.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906353 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001099335.1 |
| CDS | 627..2948 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001105865.1 |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11568 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006248606.3 , NM_001105865.1 , XM_008768865.2 | |
| Accession Version | XM_006248606.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | microprocessor complex subunit DGCR8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006248668.1 |
| CDS | 448..2769 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006248606.3 |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11568 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006248606.3 , NM_001105865.1 , XM_008768865.2 | |
| Accession Version | XM_008768865.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | microprocessor complex subunit DGCR8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008767087.1 |
| CDS | 4549..6870 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008768865.2 |
1 | ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCATGAGC CCACAGGAGA AGCAATGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The DGCR8 RNA-binding heme domain recognizes primary microRNAs by clamping the hairpin. |
Processing of microRNA primary transcripts requires heme in mammalian cells. |
Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain. |
MicroRNA-98 negatively regulates IL-10 production and endotoxin tolerance in macrophages after LPS stimulation. |
The widespread regulation of microRNA biogenesis, function and decay. |
