DGCR8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGCR8
Entrez Gene ID	54487
Full Name	DGCR8, microprocessor complex subunit
Synonyms	C22orf12,DGCRK6,Gy1,pasha
General protein information	Preferred Names DGCR8, microprocessor complex subunit Names microprocessor complex subunit DGCR8 DiGeorge syndrome critical region 8 DiGeorge syndrome critical region gene 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010].
Disorder MIM:	609030

mRNA and Protein(s)

mRNA	Protein	Name
NM_022720.6	NP_073557.3	microprocessor complex subunit DGCR8 isoform 1
NM_022720.7	NP_073557.3	microprocessor complex subunit DGCR8 isoform 1
XM_006724268.3	XP_006724331.1	microprocessor complex subunit DGCR8 isoform X1
XM_006724268.2	XP_006724331.1	microprocessor complex subunit DGCR8 isoform X1
NM_001190326.1	NP_001177255.1	microprocessor complex subunit DGCR8 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dgcr8	NP_201581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002554	XP_312385.5
Homo sapiens (human)	DGCR8	NP_073557.3
Bos taurus (cattle)	DGCR8	NP_001094674.1
Rattus norvegicus (Norway rat)	Dgcr8	NP_001099335.1
Drosophila melanogaster (fruit fly)	pasha	NP_001263150.1
Pan troglodytes (chimpanzee)	DGCR8	XP_003953874.1
	DGCR8	XP_002798321.1
Canis lupus familiaris (dog)	DGCR8	XP_543542.4
Gallus gallus (chicken)	DGCR8	XP_415079.2
Danio rerio (zebrafish)	dgcr8	NP_001116221.1
Xenopus tropicalis (tropical clawed frog)	dgcr8	NP_001153011.1

Gene Ontology
Bibliography

Related articles in PubMed

Heme enables proper positioning of Drosha and DGCR8 on primary microRNAs.
Partin AC, Ngo TD, Herrell E, Jeong BC, Hon G, Nam Y
Nature communications8(1)1737(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

La protein is an important microprocessor component regulating miRNA processing efficiency by association with DGCR8 to regulate formation of the DGCR8-Drosha complex for miRNA processing.
Title: Autoantigen La Regulates MicroRNA Processing from Stem-Loop Precursors by Association with DGCR8.

Mechanistic dissection reveals that NEAT1 broadly interacts with the NONO-PSF heterodimer as well as many other RNA-binding proteins and that multiple RNA segments in NEAT1, including a 'pseudo pri-miRNA' near its 3' end, help attract the Drosha-DGCR8 Microprocessor.
Title: NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing.

BRG1 and SMARCAL1, members of the ATP-dependent chromatin remodelling family, are shown to co-regulate the transcription of DROSHA, DGCR8, and DICER in response to double-strand DNA breaks.
Title: BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage.

this study shows an unexpected function of DGCR8 in the repair of UV-induced DNA lesions that is independent of miRNA processing.
Title: DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing.

The rs417309 and rs1640299 polymorphisms of the DGCR8 gene as well as rs6877842 of the DROSHA gene might be associated with a risk of laryngeal cancer occurrence in the Polish population.
Title: Evaluation of polymorphisms in microRNA biosynthesis genes and risk of laryngeal cancer in the Polish population.

The following DGCR8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGCR8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15211	NM_001190326.1 Latest version!	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu23169	NM_022720.7 Latest version!	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$389.50-$545.30 $779.00
OHu53621	XM_006724268.3 Latest version!	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu23169	NM_022720.6	Homo sapiens DGCR8, microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$389.50-$545.30 $779.00
OHu53621	XM_006724268.2	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGCR8 ( NM_001190326.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177255.1 Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15211
Clone ID Related Accession (Same CDS sequence)	NM_001190326.1
Accession Version	NM_001190326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	microprocessor complex subunit DGCR8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA433651.1, BC078147.1 and BQ001948.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC078147.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGCCCG AGCTACACTG 
GAAATCCTCA TCCCTGACTT TGTTAAACAG ACCTCTGAAG AGAAGCCCAA AGACAGTGAA 
GAACTCGAGT ATTTTAACCA CATCAGCATC GAGGACTCGC GGGTCTACGA GCTGACCAGC 
AAGGCTGGGC TGTTGTCTCC ATATCAGATC CTCCACGAGT GCCTTAAAAG AAACCATGGG 
ATGGGTGACA CGTCTATCAA GTTTGAAGTG GTTCCTGGGA AAAACCAGAA GAGTGAATAC 
GTCATGGCGT GTGGCAAGCA CACAGTGCGC GGGTGGTGTA AGAACAAGAG AGTTGGAAAG 
CAGTTAGCCT CACAGAAGAT CCTTCAGCTG CTGCACCCAC ATGTCAAGAA CTGGGGGTCT 
TTACTGCGCA TGTATGGCCG TGAGAGCAGC AAGATGGTCA AGCAGGAGAC ATCGGACAAG 
AGTGTGATTG AGCTGCAGCA GTATGCCAAG AAGAACAAGC CCAACCTGCA CATCCTCAGC 
AAGCTCCAAG AGGAGATGAA GAGGCTAGCT GAGGAAAGGG AGGAGACTCG AAAGAAGCCC 
AAGATGTCCA TTGTGGCGTC CGCCCAGCCT GGCGGTGAGC CCCTGTGCAC CGTGGACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177255.1
CDS	430..2652
Translation	METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	NM_001190326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190326.1

ORF Insert Sequence:

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGCCCG AGCTACACTG 
GAAATCCTCA TCCCTGACTT TGTTAAACAG ACCTCTGAAG AGAAGCCCAA AGACAGTGAA 
GAACTCGAGT ATTTTAACCA CATCAGCATC GAGGACTCGC GGGTCTACGA GCTGACCAGC 
AAGGCTGGGC TGTTGTCTCC ATATCAGATC CTCCACGAGT GCCTTAAAAG AAACCATGGG 
ATGGGTGACA CGTCTATCAA GTTTGAAGTG GTTCCTGGGA AAAACCAGAA GAGTGAATAC 
GTCATGGCGT GTGGCAAGCA CACAGTGCGC GGGTGGTGTA AGAACAAGAG AGTTGGAAAG 
CAGTTAGCCT CACAGAAGAT CCTTCAGCTG CTGCACCCAC ATGTCAAGAA CTGGGGGTCT 
TTACTGCGCA TGTATGGCCG TGAGAGCAGC AAGATGGTCA AGCAGGAGAC ATCGGACAAG 
AGTGTGATTG AGCTGCAGCA GTATGCCAAG AAGAACAAGC CCAACCTGCA CATCCTCAGC 
AAGCTCCAAG AGGAGATGAA GAGGCTAGCT GAGGAAAGGG AGGAGACTCG AAAGAAGCCC 
AAGATGTCCA TTGTGGCGTC CGCCCAGCCT GGCGGTGAGC CCCTGTGCAC CGTGGACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGCR8 ( NM_022720.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_073557.3 Homo sapiens DGCR8, microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23169
Clone ID Related Accession (Same CDS sequence)	NM_022720.6 , NM_022720.7
Accession Version	NM_022720.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	microprocessor complex subunit DGCR8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA433651.1, BC037564.1, AC006547.9 and BQ001948.1. This sequence is a reference standard in the RefSeqGene project. On Jun 16, 2010 this sequence version replaced NM_022720.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037564.1, SRR1163655.370161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGAGAA CCCAAGTGAG 
CCTTTTGGTG CCTCGGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCTACACTGG AAATCCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAAGA GAAGCCCAAA GACAGTGAAG AACTCGAGTA TTTTAACCAC 
ATCAGCATCG AGGACTCGCG GGTCTACGAG CTGACCAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCACGAGTG CCTTAAAAGA AACCATGGGA TGGGTGACAC GTCTATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATACG TCATGGCGTG TGGCAAGCAC 
ACAGTGCGCG GGTGGTGTAA GAACAAGAGA GTTGGAAAGC AGTTAGCCTC ACAGAAGATC 
CTTCAGCTGC TGCACCCACA TGTCAAGAAC TGGGGGTCTT TACTGCGCAT GTATGGCCGT 
GAGAGCAGCA AGATGGTCAA GCAGGAGACA TCGGACAAGA GTGTGATTGA GCTGCAGCAG 
TATGCCAAGA AGAACAAGCC CAACCTGCAC ATCCTCAGCA AGCTCCAAGA GGAGATGAAG 
AGGCTAGCTG AGGAAAGGGA GGAGACTCGA AAGAAGCCCA AGATGTCCAT TGTGGCGTCC 
GCCCAGCCTG GCGGTGAGCC CCTGTGCACC GTGGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073557.3
CDS	430..2751
Translation	METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	NM_022720.6
Organism	Homo sapiens(human)
Definition	Homo sapiens DGCR8, microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022720.6

ORF Insert Sequence:

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGAGAA CCCAAGTGAG 
CCTTTTGGTG CCTCGGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCTACACTGG AAATCCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAAGA GAAGCCCAAA GACAGTGAAG AACTCGAGTA TTTTAACCAC 
ATCAGCATCG AGGACTCGCG GGTCTACGAG CTGACCAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCACGAGTG CCTTAAAAGA AACCATGGGA TGGGTGACAC GTCTATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATACG TCATGGCGTG TGGCAAGCAC 
ACAGTGCGCG GGTGGTGTAA GAACAAGAGA GTTGGAAAGC AGTTAGCCTC ACAGAAGATC 
CTTCAGCTGC TGCACCCACA TGTCAAGAAC TGGGGGTCTT TACTGCGCAT GTATGGCCGT 
GAGAGCAGCA AGATGGTCAA GCAGGAGACA TCGGACAAGA GTGTGATTGA GCTGCAGCAG 
TATGCCAAGA AGAACAAGCC CAACCTGCAC ATCCTCAGCA AGCTCCAAGA GGAGATGAAG 
AGGCTAGCTG AGGAAAGGGA GGAGACTCGA AAGAAGCCCA AGATGTCCAT TGTGGCGTCC 
GCCCAGCCTG GCGGTGAGCC CCTGTGCACC GTGGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGCR8 ( NM_022720.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_073557.3 Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.

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CloneID	OHu23169
Clone ID Related Accession (Same CDS sequence)	NM_022720.6 , NM_022720.7
Accession Version	NM_022720.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	microprocessor complex subunit DGCR8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA433651.1, BC037564.1, AC006547.9 and BQ001948.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_022720.6. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037564.1, SRR1163655.370161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000351989.8/ ENSP00000263209.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGAGAA CCCAAGTGAG 
CCTTTTGGTG CCTCGGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCTACACTGG AAATCCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAAGA GAAGCCCAAA GACAGTGAAG AACTCGAGTA TTTTAACCAC 
ATCAGCATCG AGGACTCGCG GGTCTACGAG CTGACCAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCACGAGTG CCTTAAAAGA AACCATGGGA TGGGTGACAC GTCTATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATACG TCATGGCGTG TGGCAAGCAC 
ACAGTGCGCG GGTGGTGTAA GAACAAGAGA GTTGGAAAGC AGTTAGCCTC ACAGAAGATC 
CTTCAGCTGC TGCACCCACA TGTCAAGAAC TGGGGGTCTT TACTGCGCAT GTATGGCCGT 
GAGAGCAGCA AGATGGTCAA GCAGGAGACA TCGGACAAGA GTGTGATTGA GCTGCAGCAG 
TATGCCAAGA AGAACAAGCC CAACCTGCAC ATCCTCAGCA AGCTCCAAGA GGAGATGAAG 
AGGCTAGCTG AGGAAAGGGA GGAGACTCGA AAGAAGCCCA AGATGTCCAT TGTGGCGTCC 
GCCCAGCCTG GCGGTGAGCC CCTGTGCACC GTGGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073557.3
CDS	421..2742
Translation	METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	NM_022720.7
Organism	Homo sapiens(human)
Definition	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022720.7

ORF Insert Sequence:

ATGGAGACAG ATGAGAGCCC CTCTCCGCTC CCGTGTGGGC CCGCAGGAGA AGCGGTGATG 
GAGAGCCGAG CTCGCCCCTT CCAAGCGCTG CCCCGTGAGC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGGTGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CTCCCTGCTG AGGACCCCTT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACCGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACCGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGCA 
GAGCGCGACG TGCGGGCGGA GTGCGGTCTG CTCCTTAGCC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTGAC GGGGTAGGCA TAGGGGGTGA GAGTGCTGAT 
AAGAAGGATG AGGAGAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAGCAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAGAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CCCCTACGAG 
GATGACTTTG ACAACGATGT GGATGCTCTG CTGGAAGAAG GCCTTTGTGC CCCCAAAAAG 
AGGCGAACAG AGGAAAAATA TGGCGGAGAC AGCGACCATC CGTCCGATGG AGAGACAAGT 
GTGCAGCCGA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG CCCACCTACA 
GAGCCGCTGC CCGACGGGTG GATCATGACA TTCCATAACT CTGGAGTCCC GGTGTACCTA 
CACAGAGAGT CTCGGGTGGT CACCTGGTCC AGGCCATACT TCTTGGGAAC GGGAAGCATA 
CGGAAACACG ACCCTCCTCT GAGTAGCATC CCTTGTCTGC ATTATAAGAA AATGAAGGAC 
AACGAGGAAC GGGAGCAAAG CAGTGACCTC ACCCCTAGTG GGGATGTGTC CCCCGTCAAG 
CCCCTGAGCC GATCTGCAGA GCTGGAGTTT CCCCTGGATG AGCCTGACTC TATGGGTGCT 
GACCCGGGGC CCCCGGACGA GAAAGACCCA CTAGGGGCTG AGGCAGCCCC TGGGGCCCTG 
GGGCAGGTGA AGGCCAAAGT CGAGGTGTGC AAAGATGAAT CCGTTGATCT CGAGGAATTT 
CGAAGCTACC TGGAGAAGCG TTTTGACTTT GAGCAAGTTA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGGCAATT CAATCGGGAA ATGAAGCGGA AGCAGGCGGA GTCCGAGAGG 
CCCATCTTGC CAGCCAATCA GAAGCTCATT ACTTTATCAG TGCAAGATGC ACCCACAAAG 
AAAGAGTTTG TTATTAACCC CAACGGGAAA TCCGAGGTCT GCATCCTGCA CGAGTACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTCTAT AATTTCTTTG AATGTGAGAA CCCAAGTGAG 
CCTTTTGGTG CCTCGGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCTACACTGG AAATCCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAAGA GAAGCCCAAA GACAGTGAAG AACTCGAGTA TTTTAACCAC 
ATCAGCATCG AGGACTCGCG GGTCTACGAG CTGACCAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCACGAGTG CCTTAAAAGA AACCATGGGA TGGGTGACAC GTCTATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATACG TCATGGCGTG TGGCAAGCAC 
ACAGTGCGCG GGTGGTGTAA GAACAAGAGA GTTGGAAAGC AGTTAGCCTC ACAGAAGATC 
CTTCAGCTGC TGCACCCACA TGTCAAGAAC TGGGGGTCTT TACTGCGCAT GTATGGCCGT 
GAGAGCAGCA AGATGGTCAA GCAGGAGACA TCGGACAAGA GTGTGATTGA GCTGCAGCAG 
TATGCCAAGA AGAACAAGCC CAACCTGCAC ATCCTCAGCA AGCTCCAAGA GGAGATGAAG 
AGGCTAGCTG AGGAAAGGGA GGAGACTCGA AAGAAGCCCA AGATGTCCAT TGTGGCGTCC 
GCCCAGCCTG GCGGTGAGCC CCTGTGCACC GTGGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGCR8 ( XM_006724268.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724331.1 Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.

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CloneID	OHu53621
Clone ID Related Accession (Same CDS sequence)	XM_006724268.3 , XM_006724268.2
Accession Version	XM_006724268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006724268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA ACGAGGAACG GGAGCAAAGC AGTGACCTCA CCCCTAGTGG GGATGTGTCC 
CCCGTCAAGC CCCTGAGCCG ATCTGCAGAG CTGGAGTTTC CCCTGGATGA GCCTGACTCT 
ATGGGTGCTG ACCCGGGGCC CCCGGACGAG AAAGACCCAC TAGGGGCTGA GGCAGCCCCT 
GGGGCCCTGG GGCAGGTGAA GGCCAAAGTC GAGGTGTGCA AAGATGAATC CGTTGATCTC 
GAGGAATTTC GAAGCTACCT GGAGAAGCGT TTTGACTTTG AGCAAGTTAC TGTGAAAAAA 
TTCAGGACTT GGGCTGAGCG GCGGCAATTC AATCGGGAAA TGAAGCGGAA GCAGGCGGAG 
TCCGAGAGGC CCATCTTGCC AGCCAATCAG AAGCTCATTA CTTTATCAGT GCAAGATGCA 
CCCACAAAGA AAGAGTTTGT TATTAACCCC AACGGGAAAT CCGAGGTCTG CATCCTGCAC 
GAGTACATGC AGCGTGTCCT CAAGGTCCGC CCTGTCTATA ATTTCTTTGA ATGTGAGAAC 
CCAAGTGAGC CTTTTGGTGC CTCGGTGACC ATTGATGGTG TGACTTACGG ATCTGGAACT 
GCAAGCAGCA AAAAACTTGC GAAGAATAAA GCTGCCCGAG CTACACTGGA AATCCTCATC 
CCTGACTTTG TTAAACAGAC CTCTGAAGAG AAGCCCAAAG ACAGTGAAGA ACTCGAGTAT 
TTTAACCACA TCAGCATCGA GGACTCGCGG GTCTACGAGC TGACCAGCAA GGCTGGGCTG 
TTGTCTCCAT ATCAGATCCT CCACGAGTGC CTTAAAAGAA ACCATGGGAT GGGTGACACG 
TCTATCAAGT TTGAAGTGGT TCCTGGGAAA AACCAGAAGA GTGAATACGT CATGGCGTGT 
GGCAAGCACA CAGTGCGCGG GTGGTGTAAG AACAAGAGAG TTGGAAAGCA GTTAGCCTCA 
CAGAAGATCC TTCAGCTGCT GCACCCACAT GTCAAGAACT GGGGGTCTTT ACTGCGCATG 
TATGGCCGTG AGAGCAGCAA GATGGTCAAG CAGGAGACAT CGGACAAGAG TGTGATTGAG 
CTGCAGCAGT ATGCCAAGAA GAACAAGCCC AACCTGCACA TCCTCAGCAA GCTCCAAGAG 
GAGATGAAGA GGCTAGCTGA GGAAAGGGAG GAGACTCGAA AGAAGCCCAA GATGTCCATT 
GTGGCGTCCG CCCAGCCTGG CGGTGAGCCC CTGTGCACCG TGGACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724331.1
CDS	136..1386
Translation	MKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_006724268.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724268.3

ORF Insert Sequence:

ATGAAGGACA ACGAGGAACG GGAGCAAAGC AGTGACCTCA CCCCTAGTGG GGATGTGTCC 
CCCGTCAAGC CCCTGAGCCG ATCTGCAGAG CTGGAGTTTC CCCTGGATGA GCCTGACTCT 
ATGGGTGCTG ACCCGGGGCC CCCGGACGAG AAAGACCCAC TAGGGGCTGA GGCAGCCCCT 
GGGGCCCTGG GGCAGGTGAA GGCCAAAGTC GAGGTGTGCA AAGATGAATC CGTTGATCTC 
GAGGAATTTC GAAGCTACCT GGAGAAGCGT TTTGACTTTG AGCAAGTTAC TGTGAAAAAA 
TTCAGGACTT GGGCTGAGCG GCGGCAATTC AATCGGGAAA TGAAGCGGAA GCAGGCGGAG 
TCCGAGAGGC CCATCTTGCC AGCCAATCAG AAGCTCATTA CTTTATCAGT GCAAGATGCA 
CCCACAAAGA AAGAGTTTGT TATTAACCCC AACGGGAAAT CCGAGGTCTG CATCCTGCAC 
GAGTACATGC AGCGTGTCCT CAAGGTCCGC CCTGTCTATA ATTTCTTTGA ATGTGAGAAC 
CCAAGTGAGC CTTTTGGTGC CTCGGTGACC ATTGATGGTG TGACTTACGG ATCTGGAACT 
GCAAGCAGCA AAAAACTTGC GAAGAATAAA GCTGCCCGAG CTACACTGGA AATCCTCATC 
CCTGACTTTG TTAAACAGAC CTCTGAAGAG AAGCCCAAAG ACAGTGAAGA ACTCGAGTAT 
TTTAACCACA TCAGCATCGA GGACTCGCGG GTCTACGAGC TGACCAGCAA GGCTGGGCTG 
TTGTCTCCAT ATCAGATCCT CCACGAGTGC CTTAAAAGAA ACCATGGGAT GGGTGACACG 
TCTATCAAGT TTGAAGTGGT TCCTGGGAAA AACCAGAAGA GTGAATACGT CATGGCGTGT 
GGCAAGCACA CAGTGCGCGG GTGGTGTAAG AACAAGAGAG TTGGAAAGCA GTTAGCCTCA 
CAGAAGATCC TTCAGCTGCT GCACCCACAT GTCAAGAACT GGGGGTCTTT ACTGCGCATG 
TATGGCCGTG AGAGCAGCAA GATGGTCAAG CAGGAGACAT CGGACAAGAG TGTGATTGAG 
CTGCAGCAGT ATGCCAAGAA GAACAAGCCC AACCTGCACA TCCTCAGCAA GCTCCAAGAG 
GAGATGAAGA GGCTAGCTGA GGAAAGGGAG GAGACTCGAA AGAAGCCCAA GATGTCCATT 
GTGGCGTCCG CCCAGCCTGG CGGTGAGCCC CTGTGCACCG TGGACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGCR8 ( XM_006724268.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724331.1 Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu53621
Clone ID Related Accession (Same CDS sequence)	XM_006724268.3 , XM_006724268.2
Accession Version	XM_006724268.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006724268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA ACGAGGAACG GGAGCAAAGC AGTGACCTCA CCCCTAGTGG GGATGTGTCC 
CCCGTCAAGC CCCTGAGCCG ATCTGCAGAG CTGGAGTTTC CCCTGGATGA GCCTGACTCT 
ATGGGTGCTG ACCCGGGGCC CCCGGACGAG AAAGACCCAC TAGGGGCTGA GGCAGCCCCT 
GGGGCCCTGG GGCAGGTGAA GGCCAAAGTC GAGGTGTGCA AAGATGAATC CGTTGATCTC 
GAGGAATTTC GAAGCTACCT GGAGAAGCGT TTTGACTTTG AGCAAGTTAC TGTGAAAAAA 
TTCAGGACTT GGGCTGAGCG GCGGCAATTC AATCGGGAAA TGAAGCGGAA GCAGGCGGAG 
TCCGAGAGGC CCATCTTGCC AGCCAATCAG AAGCTCATTA CTTTATCAGT GCAAGATGCA 
CCCACAAAGA AAGAGTTTGT TATTAACCCC AACGGGAAAT CCGAGGTCTG CATCCTGCAC 
GAGTACATGC AGCGTGTCCT CAAGGTCCGC CCTGTCTATA ATTTCTTTGA ATGTGAGAAC 
CCAAGTGAGC CTTTTGGTGC CTCGGTGACC ATTGATGGTG TGACTTACGG ATCTGGAACT 
GCAAGCAGCA AAAAACTTGC GAAGAATAAA GCTGCCCGAG CTACACTGGA AATCCTCATC 
CCTGACTTTG TTAAACAGAC CTCTGAAGAG AAGCCCAAAG ACAGTGAAGA ACTCGAGTAT 
TTTAACCACA TCAGCATCGA GGACTCGCGG GTCTACGAGC TGACCAGCAA GGCTGGGCTG 
TTGTCTCCAT ATCAGATCCT CCACGAGTGC CTTAAAAGAA ACCATGGGAT GGGTGACACG 
TCTATCAAGT TTGAAGTGGT TCCTGGGAAA AACCAGAAGA GTGAATACGT CATGGCGTGT 
GGCAAGCACA CAGTGCGCGG GTGGTGTAAG AACAAGAGAG TTGGAAAGCA GTTAGCCTCA 
CAGAAGATCC TTCAGCTGCT GCACCCACAT GTCAAGAACT GGGGGTCTTT ACTGCGCATG 
TATGGCCGTG AGAGCAGCAA GATGGTCAAG CAGGAGACAT CGGACAAGAG TGTGATTGAG 
CTGCAGCAGT ATGCCAAGAA GAACAAGCCC AACCTGCACA TCCTCAGCAA GCTCCAAGAG 
GAGATGAAGA GGCTAGCTGA GGAAAGGGAG GAGACTCGAA AGAAGCCCAA GATGTCCATT 
GTGGCGTCCG CCCAGCCTGG CGGTGAGCCC CTGTGCACCG TGGACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724331.1
CDS	136..1386
Translation	MKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_006724268.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724268.2

ORF Insert Sequence:

ATGAAGGACA ACGAGGAACG GGAGCAAAGC AGTGACCTCA CCCCTAGTGG GGATGTGTCC 
CCCGTCAAGC CCCTGAGCCG ATCTGCAGAG CTGGAGTTTC CCCTGGATGA GCCTGACTCT 
ATGGGTGCTG ACCCGGGGCC CCCGGACGAG AAAGACCCAC TAGGGGCTGA GGCAGCCCCT 
GGGGCCCTGG GGCAGGTGAA GGCCAAAGTC GAGGTGTGCA AAGATGAATC CGTTGATCTC 
GAGGAATTTC GAAGCTACCT GGAGAAGCGT TTTGACTTTG AGCAAGTTAC TGTGAAAAAA 
TTCAGGACTT GGGCTGAGCG GCGGCAATTC AATCGGGAAA TGAAGCGGAA GCAGGCGGAG 
TCCGAGAGGC CCATCTTGCC AGCCAATCAG AAGCTCATTA CTTTATCAGT GCAAGATGCA 
CCCACAAAGA AAGAGTTTGT TATTAACCCC AACGGGAAAT CCGAGGTCTG CATCCTGCAC 
GAGTACATGC AGCGTGTCCT CAAGGTCCGC CCTGTCTATA ATTTCTTTGA ATGTGAGAAC 
CCAAGTGAGC CTTTTGGTGC CTCGGTGACC ATTGATGGTG TGACTTACGG ATCTGGAACT 
GCAAGCAGCA AAAAACTTGC GAAGAATAAA GCTGCCCGAG CTACACTGGA AATCCTCATC 
CCTGACTTTG TTAAACAGAC CTCTGAAGAG AAGCCCAAAG ACAGTGAAGA ACTCGAGTAT 
TTTAACCACA TCAGCATCGA GGACTCGCGG GTCTACGAGC TGACCAGCAA GGCTGGGCTG 
TTGTCTCCAT ATCAGATCCT CCACGAGTGC CTTAAAAGAA ACCATGGGAT GGGTGACACG 
TCTATCAAGT TTGAAGTGGT TCCTGGGAAA AACCAGAAGA GTGAATACGT CATGGCGTGT 
GGCAAGCACA CAGTGCGCGG GTGGTGTAAG AACAAGAGAG TTGGAAAGCA GTTAGCCTCA 
CAGAAGATCC TTCAGCTGCT GCACCCACAT GTCAAGAACT GGGGGTCTTT ACTGCGCATG 
TATGGCCGTG AGAGCAGCAA GATGGTCAAG CAGGAGACAT CGGACAAGAG TGTGATTGAG 
CTGCAGCAGT ATGCCAAGAA GAACAAGCCC AACCTGCACA TCCTCAGCAA GCTCCAAGAG 
GAGATGAAGA GGCTAGCTGA GGAAAGGGAG GAGACTCGAA AGAAGCCCAA GATGTCCATT 
GTGGCGTCCG CCCAGCCTGG CGGTGAGCCC CTGTGCACCG TGGACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heme enables proper positioning of Drosha and DGCR8 on primary microRNAs.
Nature communications8(1)1737(2017 Nov)
Partin AC,Ngo TD,Herrell E,Jeong BC,Hon G,Nam Y

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DGCR8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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