Dgcr8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgcr8
Entrez Gene ID	94223
Full Name	DGCR8, microprocessor complex subunit
Synonyms	D16H22S1742E,D16H22S788E,D16Wis2,Gy1,N41,Vo59c07,mir-1306
General protein information	Preferred Names DGCR8, microprocessor complex subunit Names microprocessor complex subunit DGCR8 DiGeorge syndrome critical region gene 8 diGeorge syndrome critical region 8 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A3\|16 11.31 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_033324.2	NP_201581.2	microprocessor complex subunit DGCR8
XM_006522795.2	XP_006522858.1	microprocessor complex subunit DGCR8 isoform X1
XM_030249352.1	XP_030105212.1	microprocessor complex subunit DGCR8 isoform X1
XM_030249354.1	XP_030105214.1	microprocessor complex subunit DGCR8 isoform X1
XM_030249355.1	XP_030105215.1	microprocessor complex subunit DGCR8 isoform X2
XM_006522795.3	XP_006522858.1	microprocessor complex subunit DGCR8 isoform X1
XM_030249353.1	XP_030105213.1	microprocessor complex subunit DGCR8 isoform X1
XM_017317176.1	XP_017172665.1	microprocessor complex subunit DGCR8 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dgcr8	NP_201581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002554	XP_312385.5
Homo sapiens (human)	DGCR8	NP_073557.3
Bos taurus (cattle)	DGCR8	NP_001094674.1
Rattus norvegicus (Norway rat)	Dgcr8	NP_001099335.1
Drosophila melanogaster (fruit fly)	pasha	NP_001263150.1
Pan troglodytes (chimpanzee)	DGCR8	XP_003953874.1
	DGCR8	XP_002798321.1
Canis lupus familiaris (dog)	DGCR8	XP_543542.4
Gallus gallus (chicken)	DGCR8	XP_415079.2
Danio rerio (zebrafish)	dgcr8	NP_001116221.1
Xenopus tropicalis (tropical clawed frog)	dgcr8	NP_001153011.1

Gene Ontology
Bibliography

Related articles in PubMed

Developmental excitatory-to-inhibitory GABA-polarity switch is disrupted in 22q11.2 deletion syndrome: a potential target for clinical therapeutics.
Amin H, Marinaro F, De Pietri Tonelli D, Berdondini L
Scientific reports7(1)15752(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that Dgcr8 mutations induce an earlier and stronger phenotype in the developing nervous system compared to Dicer mutants and that miRNA-independent functions of DGCR8 are critical for corticogenesis.
Title: MicroRNA-independent functions of DGCR8 are essential for neocortical development and TBR1 expression.

DGCR8 in the modulates of the alternative splicing of Tcf7l1 mRNA in addition to its established function in microRNA biogenesis, controlling embryonic stem cell exit from pluripotency.
Title: Noncanonical function of DGCR8 controls mESC exit from pluripotency.

this study shows that B-cell specific cre-mediated DGCR8 deletion blocks B-cell development at the transition from the pro-B to the pre-B cell stage
Title: The microprocessor component, DGCR8, is essential for early B-cell development in mice.

we suggest that DGCR8-dependent canonical microRNAs are essential for uterine development and physiological processes such as proper immune modulation, reproductive cycle, and steroid hormone responsiveness in mice.
Title: Deficiency in DGCR8-dependent canonical microRNAs causes infertility due to multiple abnormalities during uterine development in mice.

Dgcr8 is responsible for modulation of gene expression programs underlying myelin formation and maintenance as well as suppression of an injury-related gene expression program
Title: Microprocessor complex subunit DiGeorge syndrome critical region gene 8 (Dgcr8) is required for schwann cell myelination and myelin maintenance.

The following Dgcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52576	XM_017317176.1 Latest version!	Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu16699	NM_033324.2 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$454.30 $649.00
OMu16699	XM_030249355.1 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52576	XM_030249354.1 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52576	XM_030249353.1 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52576	XM_030249352.1 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52576	XM_006522795.3 Latest version!	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu52576	XM_006522795.2	Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgcr8 ( XM_006522795.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522858.1 Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_006522795.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522858.1
CDS	431..2815
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_006522795.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522795.2

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_017317176.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172665.1 Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_017317176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172665.1
CDS	4681..7065
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_017317176.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317176.1

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( NM_033324.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_201581.2 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), mRNA.

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CloneID	OMu16699
Clone ID Related Accession (Same CDS sequence)	NM_033324.2 , XM_030249355.1
Accession Version	NM_033324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB086857.1. On Jan 23, 2004 this sequence version replaced NM_033324.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB086857.1, BC062919.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGTA TTTTAACCAC 
ATCAGTATTG AGGATTCACG AGTCTATGAG CTGACAAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCATGAGTG CCTTAAAAGA AACCATGGAA TGGGTGACAC ATCCATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATATG TTATGGCATG CGGCAAACAC 
ACAGTGCGCG GGTGGTGTAA GAATAAACGA GTTGGGAAAC AATTAGCATC TCAGAAAATC 
CTTCAGCTAC TGCACCCACA TGTCAAGAAC TGGGGTTCCT TACTACGCAT GTATGGTCGT 
GAGAGCAGCA AAATGGTCAA GCAGGAGACC TCTGACAAGA GTGTGATAGA GCTACAGCAG 
TATGCCAAGA AGAACAGGCC CAACCTTCAC ATCCTGAGCA AGCTACAAGA AGAGATGAAG 
AGGCTGGCTG CAGAGCGGGA GGAGACTCGG AAGAAACCCA AGATGTCAAT TGTAGCATCT 
GCCCAGCCTG GTGGTGAGCC CTTGTGCACA GTCGATGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_201581.2
CDS	347..2668
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	NM_033324.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033324.2

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGTA TTTTAACCAC 
ATCAGTATTG AGGATTCACG AGTCTATGAG CTGACAAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCATGAGTG CCTTAAAAGA AACCATGGAA TGGGTGACAC ATCCATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATATG TTATGGCATG CGGCAAACAC 
ACAGTGCGCG GGTGGTGTAA GAATAAACGA GTTGGGAAAC AATTAGCATC TCAGAAAATC 
CTTCAGCTAC TGCACCCACA TGTCAAGAAC TGGGGTTCCT TACTACGCAT GTATGGTCGT 
GAGAGCAGCA AAATGGTCAA GCAGGAGACC TCTGACAAGA GTGTGATAGA GCTACAGCAG 
TATGCCAAGA AGAACAGGCC CAACCTTCAC ATCCTGAGCA AGCTACAAGA AGAGATGAAG 
AGGCTGGCTG CAGAGCGGGA GGAGACTCGG AAGAAACCCA AGATGTCAAT TGTAGCATCT 
GCCCAGCCTG GTGGTGAGCC CTTGTGCACA GTCGATGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_030249355.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105215.1 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu16699
Clone ID Related Accession (Same CDS sequence)	NM_033324.2 , XM_030249355.1
Accession Version	XM_030249355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGTA TTTTAACCAC 
ATCAGTATTG AGGATTCACG AGTCTATGAG CTGACAAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCATGAGTG CCTTAAAAGA AACCATGGAA TGGGTGACAC ATCCATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATATG TTATGGCATG CGGCAAACAC 
ACAGTGCGCG GGTGGTGTAA GAATAAACGA GTTGGGAAAC AATTAGCATC TCAGAAAATC 
CTTCAGCTAC TGCACCCACA TGTCAAGAAC TGGGGTTCCT TACTACGCAT GTATGGTCGT 
GAGAGCAGCA AAATGGTCAA GCAGGAGACC TCTGACAAGA GTGTGATAGA GCTACAGCAG 
TATGCCAAGA AGAACAGGCC CAACCTTCAC ATCCTGAGCA AGCTACAAGA AGAGATGAAG 
AGGCTGGCTG CAGAGCGGGA GGAGACTCGG AAGAAACCCA AGATGTCAAT TGTAGCATCT 
GCCCAGCCTG GTGGTGAGCC CTTGTGCACA GTCGATGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105215.1
CDS	4681..7002
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_030249355.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249355.1

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGTA TTTTAACCAC 
ATCAGTATTG AGGATTCACG AGTCTATGAG CTGACAAGCA AGGCTGGGCT GTTGTCTCCA 
TATCAGATCC TCCATGAGTG CCTTAAAAGA AACCATGGAA TGGGTGACAC ATCCATCAAG 
TTTGAAGTGG TTCCTGGGAA AAACCAGAAG AGTGAATATG TTATGGCATG CGGCAAACAC 
ACAGTGCGCG GGTGGTGTAA GAATAAACGA GTTGGGAAAC AATTAGCATC TCAGAAAATC 
CTTCAGCTAC TGCACCCACA TGTCAAGAAC TGGGGTTCCT TACTACGCAT GTATGGTCGT 
GAGAGCAGCA AAATGGTCAA GCAGGAGACC TCTGACAAGA GTGTGATAGA GCTACAGCAG 
TATGCCAAGA AGAACAGGCC CAACCTTCAC ATCCTGAGCA AGCTACAAGA AGAGATGAAG 
AGGCTGGCTG CAGAGCGGGA GGAGACTCGG AAGAAACCCA AGATGTCAAT TGTAGCATCT 
GCCCAGCCTG GTGGTGAGCC CTTGTGCACA GTCGATGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_030249352.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105212.1 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X2, mRNA.

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CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_030249352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105212.1
CDS	357..2741
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_030249352.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249352.1

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_030249354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105214.1 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X4, mRNA.

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CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_030249354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105214.1
CDS	347..2731
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_030249354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249354.1

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_006522795.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522858.1 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X1, mRNA.

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CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_006522795.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522858.1
CDS	430..2814
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_006522795.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522795.3

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgcr8 ( XM_030249353.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105213.1 Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X3, mRNA.

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CloneID	OMu52576
Clone ID Related Accession (Same CDS sequence)	XM_006522795.2 , XM_030249352.1 , XM_030249354.1 , XM_006522795.3 , XM_030249353.1 , XM_017317176.1
Accession Version	XM_030249353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microprocessor complex subunit DGCR8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105213.1
CDS	4277..6661
Translation	METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEILFCFQEALFGLQLDESTNRCYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV

Target ORF information:

RefSeq Version	XM_030249353.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DGCR8, microprocessor complex subunit (Dgcr8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249353.1

ORF Insert Sequence:

ATGGAGACAT ATGAGAGTCC CTCTCCTCTC CCGCGTGAGC CCGCAGGAGA AGCGATGATG 
GAGAACCGAG CTTGCCCCTT CCAAGTGCTG CCCCATGAAC AGTCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGATGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCCGAA 
CCTCCTGCCG ACGACCCATT CAACTTCTAC GGAGCTTCTC TTCTCTCCAA AGGATCCTTC 
TCTAAGGGCC GCCTCCTCAT AGACCCGAAC TGTAGTGGCC ACAGCCCGCG CACTGCCCGG 
CACGCACCTG CGGTCCGGAA GTTCTCCCCT GACCTTAAGT TGCTTAAGGA TGTAAAGATT 
AGCGTGAGCT TTACTGAGAG CTGCAGGAGT AAGGACAGGA AGGTGCTGTA CACAGGAGTA 
GAACGCAGCA CTCGGCCTGA GTGTGGCCAG CTCCTTAGTC CTGTCAGTGG GGACGTGCAT 
GCTTGTCCCT TTGGCGGGAG TGTTGGTAAT GGGGTAGGCC TAGGGGGTGA GAGTGCAGAT 
AAGAAGGATG AGGAAAATGA GCTGGATCAG GAAAAGAGAG TGGAGTATGC AGTGCTCGAT 
GAGTTAGAAG ATTTTACTGA CAATTTGGAG CTAGATGAAG AAGGAACAGG CGGGTTCACG 
GCTAAAGCAA TCGTTCAAAG AGACAGAGTG GATGAAGAGG CCTTGAATTT CTCCTATGAG 
GATGACTTTG ACAATGATGT GGACGCTTTA CTAGAAGAAG GTCTCTGTGC TCCCAAGAAG 
AGGCGAATGG AGGAAAAATA TGGCGGAGAC AGTGATCATC CATCTGATGG AGAGACAAGT 
GTACAGCCAA TGATGACCAA GATTAAAACA GTGCTCAAAA GTCGTGGCCG TCCACCTACA 
GAGCCATTGC CTGATGGATG GATCATGACT TTTCATAATT CTGGAGTCCC TGTATACCTG 
CACAGAGAGT CTCGAGTGGT CACTTGGTCC AGACCCTACT TCTTGGGAAC AGGAAGCATA 
CGGAAACATG ATCCTCCTCT AAGCAGTATC CCCTGCCTAC ATTATAAGAA AATGAAGGAC 
AATGAGGAAC GAGAACAAAA CTGTGATCTT GCCCCCAGTG GAGAGGTGTC ACCTGTCAAG 
CCCTTGGGTC GGTCTGCAGA GTTGGATTTC CCTCTGGAAG AGCCTGACTC CATGGGTGGA 
GACTCAGGGT CCATGGATGA GAAGGACCCA TTGGGGGCTG AGGCAGCCGC TGGAGCCCTG 
GGACAAGTGA AGGCTAAAGT TGAGGTGTGC AAAGATGAAT CAGTTGATCT GGAGGAATTT 
CGTAATTACC TTGAGAAGCG TTTTGACTTT GAACAAGTAA CTGTGAAAAA ATTCAGGACT 
TGGGCTGAGC GGCGTCAGTT CAACCGTGAG ATGAAGCGGA AGCAGGCCGA GTCAGAGAGG 
CCCATCCTGC CAGCCAACCA GAAGCTGATC ACTCTATCTG TACAAGATGC ACCCACAAAG 
AAAGAGTTTG TCATCAATCC CAATGGGAAG TCTGAGGTTT GCATCCTGCA CGAATACATG 
CAGCGTGTCC TCAAGGTCCG CCCTGTTTAT AATTTCTTTG AATGTGAGAA TCCAAGTGAG 
CCTTTTGGTG CCTCCGTGAC CATTGATGGT GTGACTTACG GATCTGGAAC TGCAAGCAGC 
AAAAAACTTG CGAAGAATAA AGCTGCCCGA GCCACCCTGG AAATTCTCAT CCCTGACTTT 
GTTAAACAGA CCTCTGAGGA GAAGCCTAAA GACAGTGAAG AACTGGAGAT TCTCTTCTGC 
TTTCAAGAAG CTCTGTTTGG CCTTCAGTTG GATGAGTCTA CAAACAGATG CTATTTTAAC 
CACATCAGTA TTGAGGATTC ACGAGTCTAT GAGCTGACAA GCAAGGCTGG GCTGTTGTCT 
CCATATCAGA TCCTCCATGA GTGCCTTAAA AGAAACCATG GAATGGGTGA CACATCCATC 
AAGTTTGAAG TGGTTCCTGG GAAAAACCAG AAGAGTGAAT ATGTTATGGC ATGCGGCAAA 
CACACAGTGC GCGGGTGGTG TAAGAATAAA CGAGTTGGGA AACAATTAGC ATCTCAGAAA 
ATCCTTCAGC TACTGCACCC ACATGTCAAG AACTGGGGTT CCTTACTACG CATGTATGGT 
CGTGAGAGCA GCAAAATGGT CAAGCAGGAG ACCTCTGACA AGAGTGTGAT AGAGCTACAG 
CAGTATGCCA AGAAGAACAG GCCCAACCTT CACATCCTGA GCAAGCTACA AGAAGAGATG 
AAGAGGCTGG CTGCAGAGCG GGAGGAGACT CGGAAGAAAC CCAAGATGTC AATTGTAGCA 
TCTGCCCAGC CTGGTGGTGA GCCCTTGTGC ACAGTCGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Developmental excitatory-to-inhibitory GABA-polarity switch is disrupted in 22q11.2 deletion syndrome: a potential target for clinical therapeutics.
Scientific reports7(1)15752(2017 Nov)
Amin H,Marinaro F,De Pietri Tonelli D,Berdondini L

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Dgcr8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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