pasha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pasha
Entrez Gene ID	43728
Full Name	partner of drosha
Synonyms	CG1800,DGCR8,Dmel\CG1800,PASHA,Pasha,cg1800,dmDGCR8
General protein information	Preferred Names partner of drosha Names CG1800 gene product from transcript CG1800-RA CG1800-PA CG1800-PB CG1800-PC DiGeorge critical region 8 pasha-PA pasha-PB pasha-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D1-100D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276220.1	NP_001263149.1	partner of drosha, isoform B
NM_143622.3	NP_651879.1	partner of drosha, isoform A
NM_001276221.1	NP_001263150.1	partner of drosha, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dgcr8	NP_201581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002554	XP_312385.5
Homo sapiens (human)	DGCR8	NP_073557.3
Bos taurus (cattle)	DGCR8	NP_001094674.1
Rattus norvegicus (Norway rat)	Dgcr8	NP_001099335.1
Drosophila melanogaster (fruit fly)	pasha	NP_001263150.1
Pan troglodytes (chimpanzee)	DGCR8	XP_003953874.1
	DGCR8	XP_002798321.1
Canis lupus familiaris (dog)	DGCR8	XP_543542.4
Gallus gallus (chicken)	DGCR8	XP_415079.2
Danio rerio (zebrafish)	dgcr8	NP_001116221.1
Xenopus tropicalis (tropical clawed frog)	dgcr8	NP_001153011.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosha and Pasha are required for coordinated development of somatic and germ cell precursors in the larval ovary. Loss of Drosha or Pasha function perturbs somatic precursor development, causing defects in formation of terminal filaments.
Title: MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis.

report clear phenotypic differences between drosha and pasha/dgcr8 null alleles in two postembryonic lineages in the Drosophila brain
Title: Drosha-independent DGCR8/Pasha pathway regulates neuronal morphogenesis.

Excess Drosha cleaves pasha/DGCR8 primary transcripts and leads to a reduction in pasha/DGCR8 mRNA levels and Pasha/DGCR8 synthesis.
Title: Genome-wide identification of targets of the drosha-pasha/DGCR8 complex.

Pasha is essential for the biogenesis of canonical miRNAs but is dispensable for the processing and function of mirtron-derived regulatory RNAs.
Title: A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways.

DGCR8 is required for the maturation of miRNA primary transcripts.
Title: The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.

The following pasha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pasha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06778	NM_143622.3 Latest version!	Drosophila melanogaster partner of drosha (pasha), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06778	NM_001276221.1 Latest version!	Drosophila melanogaster partner of drosha (pasha), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07746	NM_001276220.1 Latest version!	Drosophila melanogaster partner of drosha (pasha), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pasha ( NM_143622.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651879.1 Drosophila melanogaster partner of drosha (pasha), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06778
Clone ID Related Accession (Same CDS sequence)	NM_143622.3 , NM_001276221.1
Accession Version	NM_143622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of drosha, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143622.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA AGCCGCTGGC CGACTGCCCG GACTCTGGGC AGGTCCGCAA GCGTCCCCGG 
CTGGAGGACG AGGAGCAGGA GCTCAAGGTG GCCGGCTTTT CGCTGAGCGC CATCGAGGAA 
ATGCGGCGGA CCAGGGACAA CGAGCTGCAG AACGAACCCA TGGACCAGCA ACTGCGACAG 
TTCCAGGTTC TCGACGAAGT CGGCTCTGGC AGCGATGAGG ATGATGAGTC AGACAACGCT 
AGCGAGCAGC AGGGCGGAAG GTATGGAGAA GGAGGCGACG AGGAGGAAGA GGCGGCTGAT 
GGTGACGGCG AAGAATACGA TTCCTCGGAA TTCGATGATG ACGAGATCGA GAATCTGCTG 
GACGAGAAGC TGCCCGAAGA GCTCCGCGAG TCAAAGCAGC CCAAATACGA GCAGCGTTTC 
AAGACTGTGC TGGAAGAAAA GCGCCTCAAC CACTTTGAAG TCCTACCCGA GGGATGGGTG 
CAGGTGACCC ACAACAGTGG AATGCCGCTC TTCTTGCATC GCAAAACTCG CGTCTGTTGC 
GCATCGAGGC CCTATTTTCT CGGCACTGGA AGTGCCCGCA AGCATGCAGT GCCCTTGGGA 
GCAATCCCCT GTCTTAACTA CAGGCGGGCT TTGGAGGAAG AGTCGGAGGT GGAGCAACAA 
AAGACGGATG GAGCGCCGCC AGTGGCTGTA TGTCCCATGG CCGGAGCTTC CGCTTTACCG 
GCCGAAGCCA TGGACGTGGA GGGAAAAGAG AACAACGAAG GCAGCACCGA TTCCAAAACC 
CAACCAGATG GAATAACTGC CCTTATGCCG GCTGCTAAAA TTGTTACGGT CAACGAGAAT 
ACCCAAAAGG AATCCATTAC ACCGGAGCAA CTAAACACCT ACTGCCAGAA ACTCTTCAAA 
TTCAAGGTGA TTCGTGTGCT GCGATTTCGG AGTTGGAATG CTCGCCGCAA GTTCACCAAA 
AACCGCAAAC ACATCAAGAA TATTCAACGA CCCACGCTGC CGGATGGCAC TAAGCTTATT 
AAGTTCCCAA TACTAGCGAA TGCTGGCGAA GGATCAACGA ACCCGCGCGG TCGGAAGGAG 
TGGATCATGA ATCCCAATGG CAAGAGCTTC GTCTGCATCC TGCACGAGTA CGTGCAGCAT 
GCTCTGAAAA CGCAGCCCAC CTATGAGTTC AAGGAGCTCC AAAACGCAGC CACGCCTTAC 
TCCGCCACCG TATCGGTGAA CAATCTCAAG TATGGCACGG GCTATGGCAC CAGTAAAAAG 
CAGGCCAAGT CAGAGGCGGC TCGGGAAACG CTGGAAATAC TCATTCCCGA TGTCAAGGAC 
AAGATAACGG GCAACAATAA GGACCAAAAG AGCGGCACTG CGAAGAAAGC GCAGAGCGAT 
CTGTCTGTTT TCGATGACAT CCGCATCGAA GATCCCCGAG TCACCGAATT CTGCAACAAG 
ACGACGGAGC CATCTCCCAA CGCCATTCTG CTGACTTGCT TGCAGCGCAA CTACGGCAGC 
GATGTTCAGA TCAGCCAGGA GATCAACCGC ACCGCGAATA ACAAGAACGA GTTTACCATG 
ACCGTGGGCA AACATACTGC CAAAGTGGTG TGTAAAAACA AGCGGGAGGG CAAGCAGTTG 
GCTTCGCAAG CAATCCTCCA GATTTTGCAT CCGCACATCC AGACCTGGGG TTCCTTGTTG 
CGCCTCTACG GCAACAACTC CATAAAGACC TTCAAGGAGA AGAAGCTGGA GGAGCAGGAG 
ATCACCGTGC TGCAGAGCAA GGCGGCAGTC AACCAACCCA ACTACGCCAT ACTGGACAAA 
CTTAAATCCG AGATGTTGAA ACTGTGTGCC AAGCAGGAGA GCGTCCTAAC CATGGGCACT 
TTTGTGCCGC CAAGCGACGT CGATCTGCCC ACTTCCTCCG GGTCCAATTT GAACAACGTG 
GAACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651879.1
CDS	562..2490
Translation	MAEKPLADCPDSGQVRKRPRLEDEEQELKVAGFSLSAIEEMRRTRDNELQNEPMDQQLRQFQVLDEVGSGSDEDDESDNASEQQGGRYGEGGDEEEEAADGDGEEYDSSEFDDDEIENLLDEKLPEELRESKQPKYEQRFKTVLEEKRLNHFEVLPEGWVQVTHNSGMPLFLHRKTRVCCASRPYFLGTGSARKHAVPLGAIPCLNYRRALEEESEVEQQKTDGAPPVAVCPMAGASALPAEAMDVEGKENNEGSTDSKTQPDGITALMPAAKIVTVNENTQKESITPEQLNTYCQKLFKFKVIRVLRFRSWNARRKFTKNRKHIKNIQRPTLPDGTKLIKFPILANAGEGSTNPRGRKEWIMNPNGKSFVCILHEYVQHALKTQPTYEFKELQNAATPYSATVSVNNLKYGTGYGTSKKQAKSEAARETLEILIPDVKDKITGNNKDQKSGTAKKAQSDLSVFDDIRIEDPRVTEFCNKTTEPSPNAILLTCLQRNYGSDVQISQEINRTANNKNEFTMTVGKHTAKVVCKNKREGKQLASQAILQILHPHIQTWGSLLRLYGNNSIKTFKEKKLEEQEITVLQSKAAVNQPNYAILDKLKSEMLKLCAKQESVLTMGTFVPPSDVDLPTSSGSNLNNVEL

Target ORF information:

RefSeq Version	NM_143622.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of drosha (pasha), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143622.3

ORF Insert Sequence:

ATGGCGGAGA AGCCGCTGGC CGACTGCCCG GACTCTGGGC AGGTCCGCAA GCGTCCCCGG 
CTGGAGGACG AGGAGCAGGA GCTCAAGGTG GCCGGCTTTT CGCTGAGCGC CATCGAGGAA 
ATGCGGCGGA CCAGGGACAA CGAGCTGCAG AACGAACCCA TGGACCAGCA ACTGCGACAG 
TTCCAGGTTC TCGACGAAGT CGGCTCTGGC AGCGATGAGG ATGATGAGTC AGACAACGCT 
AGCGAGCAGC AGGGCGGAAG GTATGGAGAA GGAGGCGACG AGGAGGAAGA GGCGGCTGAT 
GGTGACGGCG AAGAATACGA TTCCTCGGAA TTCGATGATG ACGAGATCGA GAATCTGCTG 
GACGAGAAGC TGCCCGAAGA GCTCCGCGAG TCAAAGCAGC CCAAATACGA GCAGCGTTTC 
AAGACTGTGC TGGAAGAAAA GCGCCTCAAC CACTTTGAAG TCCTACCCGA GGGATGGGTG 
CAGGTGACCC ACAACAGTGG AATGCCGCTC TTCTTGCATC GCAAAACTCG CGTCTGTTGC 
GCATCGAGGC CCTATTTTCT CGGCACTGGA AGTGCCCGCA AGCATGCAGT GCCCTTGGGA 
GCAATCCCCT GTCTTAACTA CAGGCGGGCT TTGGAGGAAG AGTCGGAGGT GGAGCAACAA 
AAGACGGATG GAGCGCCGCC AGTGGCTGTA TGTCCCATGG CCGGAGCTTC CGCTTTACCG 
GCCGAAGCCA TGGACGTGGA GGGAAAAGAG AACAACGAAG GCAGCACCGA TTCCAAAACC 
CAACCAGATG GAATAACTGC CCTTATGCCG GCTGCTAAAA TTGTTACGGT CAACGAGAAT 
ACCCAAAAGG AATCCATTAC ACCGGAGCAA CTAAACACCT ACTGCCAGAA ACTCTTCAAA 
TTCAAGGTGA TTCGTGTGCT GCGATTTCGG AGTTGGAATG CTCGCCGCAA GTTCACCAAA 
AACCGCAAAC ACATCAAGAA TATTCAACGA CCCACGCTGC CGGATGGCAC TAAGCTTATT 
AAGTTCCCAA TACTAGCGAA TGCTGGCGAA GGATCAACGA ACCCGCGCGG TCGGAAGGAG 
TGGATCATGA ATCCCAATGG CAAGAGCTTC GTCTGCATCC TGCACGAGTA CGTGCAGCAT 
GCTCTGAAAA CGCAGCCCAC CTATGAGTTC AAGGAGCTCC AAAACGCAGC CACGCCTTAC 
TCCGCCACCG TATCGGTGAA CAATCTCAAG TATGGCACGG GCTATGGCAC CAGTAAAAAG 
CAGGCCAAGT CAGAGGCGGC TCGGGAAACG CTGGAAATAC TCATTCCCGA TGTCAAGGAC 
AAGATAACGG GCAACAATAA GGACCAAAAG AGCGGCACTG CGAAGAAAGC GCAGAGCGAT 
CTGTCTGTTT TCGATGACAT CCGCATCGAA GATCCCCGAG TCACCGAATT CTGCAACAAG 
ACGACGGAGC CATCTCCCAA CGCCATTCTG CTGACTTGCT TGCAGCGCAA CTACGGCAGC 
GATGTTCAGA TCAGCCAGGA GATCAACCGC ACCGCGAATA ACAAGAACGA GTTTACCATG 
ACCGTGGGCA AACATACTGC CAAAGTGGTG TGTAAAAACA AGCGGGAGGG CAAGCAGTTG 
GCTTCGCAAG CAATCCTCCA GATTTTGCAT CCGCACATCC AGACCTGGGG TTCCTTGTTG 
CGCCTCTACG GCAACAACTC CATAAAGACC TTCAAGGAGA AGAAGCTGGA GGAGCAGGAG 
ATCACCGTGC TGCAGAGCAA GGCGGCAGTC AACCAACCCA ACTACGCCAT ACTGGACAAA 
CTTAAATCCG AGATGTTGAA ACTGTGTGCC AAGCAGGAGA GCGTCCTAAC CATGGGCACT 
TTTGTGCCGC CAAGCGACGT CGATCTGCCC ACTTCCTCCG GGTCCAATTT GAACAACGTG 
GAACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pasha ( NM_001276221.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263150.1 Drosophila melanogaster partner of drosha (pasha), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06778
Clone ID Related Accession (Same CDS sequence)	NM_143622.3 , NM_001276221.1
Accession Version	NM_001276221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of drosha, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA AGCCGCTGGC CGACTGCCCG GACTCTGGGC AGGTCCGCAA GCGTCCCCGG 
CTGGAGGACG AGGAGCAGGA GCTCAAGGTG GCCGGCTTTT CGCTGAGCGC CATCGAGGAA 
ATGCGGCGGA CCAGGGACAA CGAGCTGCAG AACGAACCCA TGGACCAGCA ACTGCGACAG 
TTCCAGGTTC TCGACGAAGT CGGCTCTGGC AGCGATGAGG ATGATGAGTC AGACAACGCT 
AGCGAGCAGC AGGGCGGAAG GTATGGAGAA GGAGGCGACG AGGAGGAAGA GGCGGCTGAT 
GGTGACGGCG AAGAATACGA TTCCTCGGAA TTCGATGATG ACGAGATCGA GAATCTGCTG 
GACGAGAAGC TGCCCGAAGA GCTCCGCGAG TCAAAGCAGC CCAAATACGA GCAGCGTTTC 
AAGACTGTGC TGGAAGAAAA GCGCCTCAAC CACTTTGAAG TCCTACCCGA GGGATGGGTG 
CAGGTGACCC ACAACAGTGG AATGCCGCTC TTCTTGCATC GCAAAACTCG CGTCTGTTGC 
GCATCGAGGC CCTATTTTCT CGGCACTGGA AGTGCCCGCA AGCATGCAGT GCCCTTGGGA 
GCAATCCCCT GTCTTAACTA CAGGCGGGCT TTGGAGGAAG AGTCGGAGGT GGAGCAACAA 
AAGACGGATG GAGCGCCGCC AGTGGCTGTA TGTCCCATGG CCGGAGCTTC CGCTTTACCG 
GCCGAAGCCA TGGACGTGGA GGGAAAAGAG AACAACGAAG GCAGCACCGA TTCCAAAACC 
CAACCAGATG GAATAACTGC CCTTATGCCG GCTGCTAAAA TTGTTACGGT CAACGAGAAT 
ACCCAAAAGG AATCCATTAC ACCGGAGCAA CTAAACACCT ACTGCCAGAA ACTCTTCAAA 
TTCAAGGTGA TTCGTGTGCT GCGATTTCGG AGTTGGAATG CTCGCCGCAA GTTCACCAAA 
AACCGCAAAC ACATCAAGAA TATTCAACGA CCCACGCTGC CGGATGGCAC TAAGCTTATT 
AAGTTCCCAA TACTAGCGAA TGCTGGCGAA GGATCAACGA ACCCGCGCGG TCGGAAGGAG 
TGGATCATGA ATCCCAATGG CAAGAGCTTC GTCTGCATCC TGCACGAGTA CGTGCAGCAT 
GCTCTGAAAA CGCAGCCCAC CTATGAGTTC AAGGAGCTCC AAAACGCAGC CACGCCTTAC 
TCCGCCACCG TATCGGTGAA CAATCTCAAG TATGGCACGG GCTATGGCAC CAGTAAAAAG 
CAGGCCAAGT CAGAGGCGGC TCGGGAAACG CTGGAAATAC TCATTCCCGA TGTCAAGGAC 
AAGATAACGG GCAACAATAA GGACCAAAAG AGCGGCACTG CGAAGAAAGC GCAGAGCGAT 
CTGTCTGTTT TCGATGACAT CCGCATCGAA GATCCCCGAG TCACCGAATT CTGCAACAAG 
ACGACGGAGC CATCTCCCAA CGCCATTCTG CTGACTTGCT TGCAGCGCAA CTACGGCAGC 
GATGTTCAGA TCAGCCAGGA GATCAACCGC ACCGCGAATA ACAAGAACGA GTTTACCATG 
ACCGTGGGCA AACATACTGC CAAAGTGGTG TGTAAAAACA AGCGGGAGGG CAAGCAGTTG 
GCTTCGCAAG CAATCCTCCA GATTTTGCAT CCGCACATCC AGACCTGGGG TTCCTTGTTG 
CGCCTCTACG GCAACAACTC CATAAAGACC TTCAAGGAGA AGAAGCTGGA GGAGCAGGAG 
ATCACCGTGC TGCAGAGCAA GGCGGCAGTC AACCAACCCA ACTACGCCAT ACTGGACAAA 
CTTAAATCCG AGATGTTGAA ACTGTGTGCC AAGCAGGAGA GCGTCCTAAC CATGGGCACT 
TTTGTGCCGC CAAGCGACGT CGATCTGCCC ACTTCCTCCG GGTCCAATTT GAACAACGTG 
GAACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263150.1
CDS	111..2039
Translation	MAEKPLADCPDSGQVRKRPRLEDEEQELKVAGFSLSAIEEMRRTRDNELQNEPMDQQLRQFQVLDEVGSGSDEDDESDNASEQQGGRYGEGGDEEEEAADGDGEEYDSSEFDDDEIENLLDEKLPEELRESKQPKYEQRFKTVLEEKRLNHFEVLPEGWVQVTHNSGMPLFLHRKTRVCCASRPYFLGTGSARKHAVPLGAIPCLNYRRALEEESEVEQQKTDGAPPVAVCPMAGASALPAEAMDVEGKENNEGSTDSKTQPDGITALMPAAKIVTVNENTQKESITPEQLNTYCQKLFKFKVIRVLRFRSWNARRKFTKNRKHIKNIQRPTLPDGTKLIKFPILANAGEGSTNPRGRKEWIMNPNGKSFVCILHEYVQHALKTQPTYEFKELQNAATPYSATVSVNNLKYGTGYGTSKKQAKSEAARETLEILIPDVKDKITGNNKDQKSGTAKKAQSDLSVFDDIRIEDPRVTEFCNKTTEPSPNAILLTCLQRNYGSDVQISQEINRTANNKNEFTMTVGKHTAKVVCKNKREGKQLASQAILQILHPHIQTWGSLLRLYGNNSIKTFKEKKLEEQEITVLQSKAAVNQPNYAILDKLKSEMLKLCAKQESVLTMGTFVPPSDVDLPTSSGSNLNNVEL

Target ORF information:

RefSeq Version	NM_001276221.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of drosha (pasha), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276221.1

ORF Insert Sequence:

ATGGCGGAGA AGCCGCTGGC CGACTGCCCG GACTCTGGGC AGGTCCGCAA GCGTCCCCGG 
CTGGAGGACG AGGAGCAGGA GCTCAAGGTG GCCGGCTTTT CGCTGAGCGC CATCGAGGAA 
ATGCGGCGGA CCAGGGACAA CGAGCTGCAG AACGAACCCA TGGACCAGCA ACTGCGACAG 
TTCCAGGTTC TCGACGAAGT CGGCTCTGGC AGCGATGAGG ATGATGAGTC AGACAACGCT 
AGCGAGCAGC AGGGCGGAAG GTATGGAGAA GGAGGCGACG AGGAGGAAGA GGCGGCTGAT 
GGTGACGGCG AAGAATACGA TTCCTCGGAA TTCGATGATG ACGAGATCGA GAATCTGCTG 
GACGAGAAGC TGCCCGAAGA GCTCCGCGAG TCAAAGCAGC CCAAATACGA GCAGCGTTTC 
AAGACTGTGC TGGAAGAAAA GCGCCTCAAC CACTTTGAAG TCCTACCCGA GGGATGGGTG 
CAGGTGACCC ACAACAGTGG AATGCCGCTC TTCTTGCATC GCAAAACTCG CGTCTGTTGC 
GCATCGAGGC CCTATTTTCT CGGCACTGGA AGTGCCCGCA AGCATGCAGT GCCCTTGGGA 
GCAATCCCCT GTCTTAACTA CAGGCGGGCT TTGGAGGAAG AGTCGGAGGT GGAGCAACAA 
AAGACGGATG GAGCGCCGCC AGTGGCTGTA TGTCCCATGG CCGGAGCTTC CGCTTTACCG 
GCCGAAGCCA TGGACGTGGA GGGAAAAGAG AACAACGAAG GCAGCACCGA TTCCAAAACC 
CAACCAGATG GAATAACTGC CCTTATGCCG GCTGCTAAAA TTGTTACGGT CAACGAGAAT 
ACCCAAAAGG AATCCATTAC ACCGGAGCAA CTAAACACCT ACTGCCAGAA ACTCTTCAAA 
TTCAAGGTGA TTCGTGTGCT GCGATTTCGG AGTTGGAATG CTCGCCGCAA GTTCACCAAA 
AACCGCAAAC ACATCAAGAA TATTCAACGA CCCACGCTGC CGGATGGCAC TAAGCTTATT 
AAGTTCCCAA TACTAGCGAA TGCTGGCGAA GGATCAACGA ACCCGCGCGG TCGGAAGGAG 
TGGATCATGA ATCCCAATGG CAAGAGCTTC GTCTGCATCC TGCACGAGTA CGTGCAGCAT 
GCTCTGAAAA CGCAGCCCAC CTATGAGTTC AAGGAGCTCC AAAACGCAGC CACGCCTTAC 
TCCGCCACCG TATCGGTGAA CAATCTCAAG TATGGCACGG GCTATGGCAC CAGTAAAAAG 
CAGGCCAAGT CAGAGGCGGC TCGGGAAACG CTGGAAATAC TCATTCCCGA TGTCAAGGAC 
AAGATAACGG GCAACAATAA GGACCAAAAG AGCGGCACTG CGAAGAAAGC GCAGAGCGAT 
CTGTCTGTTT TCGATGACAT CCGCATCGAA GATCCCCGAG TCACCGAATT CTGCAACAAG 
ACGACGGAGC CATCTCCCAA CGCCATTCTG CTGACTTGCT TGCAGCGCAA CTACGGCAGC 
GATGTTCAGA TCAGCCAGGA GATCAACCGC ACCGCGAATA ACAAGAACGA GTTTACCATG 
ACCGTGGGCA AACATACTGC CAAAGTGGTG TGTAAAAACA AGCGGGAGGG CAAGCAGTTG 
GCTTCGCAAG CAATCCTCCA GATTTTGCAT CCGCACATCC AGACCTGGGG TTCCTTGTTG 
CGCCTCTACG GCAACAACTC CATAAAGACC TTCAAGGAGA AGAAGCTGGA GGAGCAGGAG 
ATCACCGTGC TGCAGAGCAA GGCGGCAGTC AACCAACCCA ACTACGCCAT ACTGGACAAA 
CTTAAATCCG AGATGTTGAA ACTGTGTGCC AAGCAGGAGA GCGTCCTAAC CATGGGCACT 
TTTGTGCCGC CAAGCGACGT CGATCTGCCC ACTTCCTCCG GGTCCAATTT GAACAACGTG 
GAACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pasha ( NM_001276220.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263149.1 Drosophila melanogaster partner of drosha (pasha), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07746
Clone ID Related Accession (Same CDS sequence)	NM_001276220.1
Accession Version	NM_001276220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of drosha, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCATGA TACCCATTAT CCGACTGGCT TGTCCAGTTG CGTCAGACAA CGCTAGCGAG 
CAGCAGGGCG GAAGGTATGG AGAAGGAGGC GACGAGGAGG AAGAGGCGGC TGATGGTGAC 
GGCGAAGAAT ACGATTCCTC GGAATTCGAT GATGACGAGA TCGAGAATCT GCTGGACGAG 
AAGCTGCCCG AAGAGCTCCG CGAGTCAAAG CAGCCCAAAT ACGAGCAGCG TTTCAAGACT 
GTGCTGGAAG AAAAGCGCCT CAACCACTTT GAAGTCCTAC CCGAGGGATG GGTGCAGGTG 
ACCCACAACA GTGGAATGCC GCTCTTCTTG CATCGCAAAA CTCGCGTCTG TTGCGCATCG 
AGGCCCTATT TTCTCGGCAC TGGAAGTGCC CGCAAGCATG CAGTGCCCTT GGGAGCAATC 
CCCTGTCTTA ACTACAGGCG GGCTTTGGAG GAAGAGTCGG AGGTGGAGCA ACAAAAGACG 
GATGGAGCGC CGCCAGTGGC TGTATGTCCC ATGGCCGGAG CTTCCGCTTT ACCGGCCGAA 
GCCATGGACG TGGAGGGAAA AGAGAACAAC GAAGGCAGCA CCGATTCCAA AACCCAACCA 
GATGGAATAA CTGCCCTTAT GCCGGCTGCT AAAATTGTTA CGGTCAACGA GAATACCCAA 
AAGGAATCCA TTACACCGGA GCAACTAAAC ACCTACTGCC AGAAACTCTT CAAATTCAAG 
GTGATTCGTG TGCTGCGATT TCGGAGTTGG AATGCTCGCC GCAAGTTCAC CAAAAACCGC 
AAACACATCA AGAATATTCA ACGACCCACG CTGCCGGATG GCACTAAGCT TATTAAGTTC 
CCAATACTAG CGAATGCTGG CGAAGGATCA ACGAACCCGC GCGGTCGGAA GGAGTGGATC 
ATGAATCCCA ATGGCAAGAG CTTCGTCTGC ATCCTGCACG AGTACGTGCA GCATGCTCTG 
AAAACGCAGC CCACCTATGA GTTCAAGGAG CTCCAAAACG CAGCCACGCC TTACTCCGCC 
ACCGTATCGG TGAACAATCT CAAGTATGGC ACGGGCTATG GCACCAGTAA AAAGCAGGCC 
AAGTCAGAGG CGGCTCGGGA AACGCTGGAA ATACTCATTC CCGATGTCAA GGACAAGATA 
ACGGGCAACA ATAAGGACCA AAAGAGCGGC ACTGCGAAGA AAGCGCAGAG CGATCTGTCT 
GTTTTCGATG ACATCCGCAT CGAAGATCCC CGAGTCACCG AATTCTGCAA CAAGACGACG 
GAGCCATCTC CCAACGCCAT TCTGCTGACT TGCTTGCAGC GCAACTACGG CAGCGATGTT 
CAGATCAGCC AGGAGATCAA CCGCACCGCG AATAACAAGA ACGAGTTTAC CATGACCGTG 
GGCAAACATA CTGCCAAAGT GGTGTGTAAA AACAAGCGGG AGGGCAAGCA GTTGGCTTCG 
CAAGCAATCC TCCAGATTTT GCATCCGCAC ATCCAGACCT GGGGTTCCTT GTTGCGCCTC 
TACGGCAACA ACTCCATAAA GACCTTCAAG GAGAAGAAGC TGGAGGAGCA GGAGATCACC 
GTGCTGCAGA GCAAGGCGGC AGTCAACCAA CCCAACTACG CCATACTGGA CAAACTTAAA 
TCCGAGATGT TGAAACTGTG TGCCAAGCAG GAGAGCGTCC TAACCATGGG CACTTTTGTG 
CCGCCAAGCG ACGTCGATCT GCCCACTTCC TCCGGGTCCA ATTTGAACAA CGTGGAACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263149.1
CDS	24..1766
Translation	MRMIPIIRLACPVASDNASEQQGGRYGEGGDEEEEAADGDGEEYDSSEFDDDEIENLLDEKLPEELRESKQPKYEQRFKTVLEEKRLNHFEVLPEGWVQVTHNSGMPLFLHRKTRVCCASRPYFLGTGSARKHAVPLGAIPCLNYRRALEEESEVEQQKTDGAPPVAVCPMAGASALPAEAMDVEGKENNEGSTDSKTQPDGITALMPAAKIVTVNENTQKESITPEQLNTYCQKLFKFKVIRVLRFRSWNARRKFTKNRKHIKNIQRPTLPDGTKLIKFPILANAGEGSTNPRGRKEWIMNPNGKSFVCILHEYVQHALKTQPTYEFKELQNAATPYSATVSVNNLKYGTGYGTSKKQAKSEAARETLEILIPDVKDKITGNNKDQKSGTAKKAQSDLSVFDDIRIEDPRVTEFCNKTTEPSPNAILLTCLQRNYGSDVQISQEINRTANNKNEFTMTVGKHTAKVVCKNKREGKQLASQAILQILHPHIQTWGSLLRLYGNNSIKTFKEKKLEEQEITVLQSKAAVNQPNYAILDKLKSEMLKLCAKQESVLTMGTFVPPSDVDLPTSSGSNLNNVEL

Target ORF information:

RefSeq Version	NM_001276220.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of drosha (pasha), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276220.1

ORF Insert Sequence:

ATGCGCATGA TACCCATTAT CCGACTGGCT TGTCCAGTTG CGTCAGACAA CGCTAGCGAG 
CAGCAGGGCG GAAGGTATGG AGAAGGAGGC GACGAGGAGG AAGAGGCGGC TGATGGTGAC 
GGCGAAGAAT ACGATTCCTC GGAATTCGAT GATGACGAGA TCGAGAATCT GCTGGACGAG 
AAGCTGCCCG AAGAGCTCCG CGAGTCAAAG CAGCCCAAAT ACGAGCAGCG TTTCAAGACT 
GTGCTGGAAG AAAAGCGCCT CAACCACTTT GAAGTCCTAC CCGAGGGATG GGTGCAGGTG 
ACCCACAACA GTGGAATGCC GCTCTTCTTG CATCGCAAAA CTCGCGTCTG TTGCGCATCG 
AGGCCCTATT TTCTCGGCAC TGGAAGTGCC CGCAAGCATG CAGTGCCCTT GGGAGCAATC 
CCCTGTCTTA ACTACAGGCG GGCTTTGGAG GAAGAGTCGG AGGTGGAGCA ACAAAAGACG 
GATGGAGCGC CGCCAGTGGC TGTATGTCCC ATGGCCGGAG CTTCCGCTTT ACCGGCCGAA 
GCCATGGACG TGGAGGGAAA AGAGAACAAC GAAGGCAGCA CCGATTCCAA AACCCAACCA 
GATGGAATAA CTGCCCTTAT GCCGGCTGCT AAAATTGTTA CGGTCAACGA GAATACCCAA 
AAGGAATCCA TTACACCGGA GCAACTAAAC ACCTACTGCC AGAAACTCTT CAAATTCAAG 
GTGATTCGTG TGCTGCGATT TCGGAGTTGG AATGCTCGCC GCAAGTTCAC CAAAAACCGC 
AAACACATCA AGAATATTCA ACGACCCACG CTGCCGGATG GCACTAAGCT TATTAAGTTC 
CCAATACTAG CGAATGCTGG CGAAGGATCA ACGAACCCGC GCGGTCGGAA GGAGTGGATC 
ATGAATCCCA ATGGCAAGAG CTTCGTCTGC ATCCTGCACG AGTACGTGCA GCATGCTCTG 
AAAACGCAGC CCACCTATGA GTTCAAGGAG CTCCAAAACG CAGCCACGCC TTACTCCGCC 
ACCGTATCGG TGAACAATCT CAAGTATGGC ACGGGCTATG GCACCAGTAA AAAGCAGGCC 
AAGTCAGAGG CGGCTCGGGA AACGCTGGAA ATACTCATTC CCGATGTCAA GGACAAGATA 
ACGGGCAACA ATAAGGACCA AAAGAGCGGC ACTGCGAAGA AAGCGCAGAG CGATCTGTCT 
GTTTTCGATG ACATCCGCAT CGAAGATCCC CGAGTCACCG AATTCTGCAA CAAGACGACG 
GAGCCATCTC CCAACGCCAT TCTGCTGACT TGCTTGCAGC GCAACTACGG CAGCGATGTT 
CAGATCAGCC AGGAGATCAA CCGCACCGCG AATAACAAGA ACGAGTTTAC CATGACCGTG 
GGCAAACATA CTGCCAAAGT GGTGTGTAAA AACAAGCGGG AGGGCAAGCA GTTGGCTTCG 
CAAGCAATCC TCCAGATTTT GCATCCGCAC ATCCAGACCT GGGGTTCCTT GTTGCGCCTC 
TACGGCAACA ACTCCATAAA GACCTTCAAG GAGAAGAAGC TGGAGGAGCA GGAGATCACC 
GTGCTGCAGA GCAAGGCGGC AGTCAACCAA CCCAACTACG CCATACTGGA CAAACTTAAA 
TCCGAGATGT TGAAACTGTG TGCCAAGCAG GAGAGCGTCC TAACCATGGG CACTTTTGTG 
CCGCCAAGCG ACGTCGATCT GCCCACTTCC TCCGGGTCCA ATTTGAACAA CGTGGAACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pasha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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