Cryab cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryab
Entrez Gene ID	25420
Full Name	crystallin, alpha B
Synonyms	AACRYA
General protein information	Preferred Names crystallin, alpha B Names alpha-crystallin B chain Crystallin, alpha polypeptide 2 alpha(B)-crystallin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q23
Summary	This gene encodes subunit b, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. Alternate promoter usage results in different transcript variants encoding the same protein. [provided by RefSeq, Sep 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012935.4	NP_037067.1	alpha-crystallin B chain

Pathways from BioSystems

Homologies

Homo sapiens (human)	CRYAB	NP_001876.1
	CRYAB	XP_002799808.1
Mus musculus (house mouse)	Cryab	NP_034094.1
Danio rerio (zebrafish)	cryabb	NP_001002670.1
Canis lupus familiaris (dog)	CRYAB	XP_862258.2
Bos taurus (cattle)	CRYAB	NP_776715.1
Gallus gallus (chicken)	CRYAB	NP_990507.1
Pan troglodytes (chimpanzee)	CRYAB	XP_508752.2
Rattus norvegicus (Norway rat)	Cryab	NP_037067.1
Drosophila melanogaster (fruit fly)	l(2)efl	NP_523827.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007162	XP_308606.2
Xenopus tropicalis (tropical clawed frog)	cryab	XP_002932964.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylation of ?B-crystallin in the myocardium: Analysis of relations with aging and cardiomyopathy.
Muraleva NA, Devyatkin VA, Kolosova NG
Experimental gerontology9526-33(2017 Sep)

Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin.
Roquemore EP, Chevrier MR, Cotter RJ, Hart GW
Biochemistry35(11)3578-86(1996 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HspB5 prevented heat shock-induced rarefaction of dendrites.
Title: HspB5/?B-crystallin increases dendritic complexity and protects the dendritic arbor during heat shock in cultured rat hippocampal neurons.

results revealed a new role for CryAB, as a modulator of Shp2 phosphatase activity during a functionally relevant stimulus in cardiomyocytes.
Title: ?B-Crystallin interacts and attenuates the tyrosine phosphatase activity of Shp2 in cardiomyocytes under mechanical stress.

These results were confirmed by analysis with STRING 9.1 software, which indicated that Hsp27 and aB-crystallin are co-expressed in rat myocardial cells
Title: Expression profiles of heat shock protein 27 and ?B-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo.

alphaB-crystallin protects rat articular chondrocytes against CK2 inhibition-induced apoptosis.
Title: Alpha B-Crystallin Protects Rat Articular Chondrocytes against Casein Kinase II Inhibition-Induced Apoptosis.

data suggest that HspB1 and HspB5 might be of protective value for retinal ganglion cells after optic nerve injury and that this process might be regulated by phosphorylation at serine 86 of HspB1 and serine 19 and serine 45 of HspB5.
Title: Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/?B-crystallin in the rat retina upon optic nerve injury.

The following Cryab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43109	NM_012935.4 Latest version!	Rattus norvegicus crystallin, alpha B (Cryab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryab ( NM_012935.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037067.1 Rattus norvegicus crystallin, alpha B (Cryab), mRNA.

Price & Availability↑

CloneID	ORa43109
Clone ID Related Accession (Same CDS sequence)	NM_012935.4
Accession Version	NM_012935.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	alpha-crystallin B chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M55534.1 and CK357656.1. On Sep 16, 2014 this sequence version replaced NM_012935.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ228589.1, M55534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 8639509 regulatory uORF :: PMID: 2176207 ##RefSeq-Attributes-END##

ATGGACATAG CCATCCACCA CCCCTGGATC CGGCGTCCCT TCTTTCCTTT CCACTCCCCA 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGACCT CTTCTCTACA 
GCCACTTCCC TGAGCCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCTAGCTGG 
ATTGACACTG GGCTCTCAGA GATGCGTATG GAGAAGGACA GGTTCTCTGT GAACCTGGAC 
GTGAAGCACT TCTCTCCAGA GGAACTCAAA GTCAAGGTTC TGGGAGACGT GATTGAGGTG 
CACGGCAAGC ACGAAGAGCG CCAGGACGAA CATGGCTTCA TCTCCAGGGA GTTCCACAGG 
AAGTACCGGA TCCCAGCCGA CGTGGATCCT CTCACCATTA CTTCTTCCCT GTCATCGGAT 
GGAGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGCCTCTG GCCCTGAGCG CACCATTCCC 
ATCACCCGTG AAGAGAAGCC TGCTGTCACT GCAGCCCCTA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037067.1
CDS	330..857
Translation	MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFSTATSLSPFYLRPPSFLRAPSWIDTGLSEMRMEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQASGPERTIPITREEKPAVTAAPKK

Target ORF information:

RefSeq Version	NM_012935.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, alpha B (Cryab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012935.4

ORF Insert Sequence:

ATGGACATAG CCATCCACCA CCCCTGGATC CGGCGTCCCT TCTTTCCTTT CCACTCCCCA 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGACCT CTTCTCTACA 
GCCACTTCCC TGAGCCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCTAGCTGG 
ATTGACACTG GGCTCTCAGA GATGCGTATG GAGAAGGACA GGTTCTCTGT GAACCTGGAC 
GTGAAGCACT TCTCTCCAGA GGAACTCAAA GTCAAGGTTC TGGGAGACGT GATTGAGGTG 
CACGGCAAGC ACGAAGAGCG CCAGGACGAA CATGGCTTCA TCTCCAGGGA GTTCCACAGG 
AAGTACCGGA TCCCAGCCGA CGTGGATCCT CTCACCATTA CTTCTTCCCT GTCATCGGAT 
GGAGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGCCTCTG GCCCTGAGCG CACCATTCCC 
ATCACCCGTG AAGAGAAGCC TGCTGTCACT GCAGCCCCTA AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylation of ?B-crystallin in the myocardium: Analysis of relations with aging and cardiomyopathy.
Experimental gerontology9526-33(2017 Sep)
Muraleva NA,Devyatkin VA,Kolosova NG

Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin.
Biochemistry35(11)3578-86(1996 Mar)
Roquemore EP,Chevrier MR,Cotter RJ,Hart GW

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Cryab cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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