l(2)efl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(2)efl
Entrez Gene ID	37744
Full Name	lethal (2) essential for life
Synonyms	CG4533,CryAB,Cryab,Dmel19.8,Dmel\CG4533,L(2)EFL,L(2)efl,el(2)13-1,l(2)13-1,l(2)59Fa,l(2)Efl
General protein information	Preferred Names lethal (2) essential for life Names CG4533 gene product from transcript CG4533-RA CG4533-PA CG4533-PC l(2)efl-PA l(2)efl-PC lethal(2)essential for life
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59F6-59F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274227.1	NP_001261156.1	lethal (2) essential for life, isoform C
NM_079103.3	NP_523827.1	lethal (2) essential for life, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	CRYAB	NP_001876.1
	CRYAB	XP_002799808.1
Mus musculus (house mouse)	Cryab	NP_034094.1
Danio rerio (zebrafish)	cryabb	NP_001002670.1
Canis lupus familiaris (dog)	CRYAB	XP_862258.2
Bos taurus (cattle)	CRYAB	NP_776715.1
Gallus gallus (chicken)	CRYAB	NP_990507.1
Pan troglodytes (chimpanzee)	CRYAB	XP_508752.2
Rattus norvegicus (Norway rat)	Cryab	NP_037067.1
Drosophila melanogaster (fruit fly)	l(2)efl	NP_523827.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007162	XP_308606.2
Xenopus tropicalis (tropical clawed frog)	cryab	XP_002932964.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(2)efl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)efl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11072	NM_001274227.1 Latest version!	Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11072	NM_079103.3 Latest version!	Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(2)efl ( NM_001274227.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261156.1 Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11072
Clone ID Related Accession (Same CDS sequence)	NM_001274227.1 , NM_079103.3
Accession Version	NM_001274227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) essential for life, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGTAG TGCCACTGAT GTTCCGTGAC TGGTGGGATG AGCTCGACTT TCCAATGCGC 
ACCTCGCGCC TCTTGGACCA GCACTTCGGT CAGGGACTGA AGAGGGATGA CCTCATGTCC 
TCCGTGTGGA ACTCCCGCCC CACAGTGCTG CGATCGGGAT ACCTGCGCCC CTGGCACACG 
AACAGCCTGC AGAAGCAGGA GTCCGGTTCC ACCCTCAACA TCGACAGCGA GAAGTTCGAG 
GTCATTCTGG ATGTGCAGCA GTTCTCGCCA TCCGAGATCA CCGTCAAGGT GGCCGACAAG 
TTCGTCATCG TGGAGGGAAA GCATGAGGAG AAGCAGGACG AGCACGGATA TGTCTCCCGC 
CAGTTCTCCA GACGCTACCA GCTGCCAAGT GATGTCAACC CCGACACGGT CACATCGTCC 
CTCTCCTCCG ATGGCCTGCT GACCATCAAG GCGCCAATGA AGGCGCTGCC TCCTCCGCAG 
ACTGAACGCC TTGTGCAGAT CACACAGACT GGTCCCTCCT CCAAGGAGGA CAACGCCAAG 
AAGGTGGAGA CCTCCACCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261156.1
CDS	72..635
Translation	MSVVPLMFRDWWDELDFPMRTSRLLDQHFGQGLKRDDLMSSVWNSRPTVLRSGYLRPWHTNSLQKQESGSTLNIDSEKFEVILDVQQFSPSEITVKVADKFVIVEGKHEEKQDEHGYVSRQFSRRYQLPSDVNPDTVTSSLSSDGLLTIKAPMKALPPPQTERLVQITQTGPSSKEDNAKKVETSTA

Target ORF information:

RefSeq Version	NM_001274227.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274227.1

ORF Insert Sequence:

ATGTCCGTAG TGCCACTGAT GTTCCGTGAC TGGTGGGATG AGCTCGACTT TCCAATGCGC 
ACCTCGCGCC TCTTGGACCA GCACTTCGGT CAGGGACTGA AGAGGGATGA CCTCATGTCC 
TCCGTGTGGA ACTCCCGCCC CACAGTGCTG CGATCGGGAT ACCTGCGCCC CTGGCACACG 
AACAGCCTGC AGAAGCAGGA GTCCGGTTCC ACCCTCAACA TCGACAGCGA GAAGTTCGAG 
GTCATTCTGG ATGTGCAGCA GTTCTCGCCA TCCGAGATCA CCGTCAAGGT GGCCGACAAG 
TTCGTCATCG TGGAGGGAAA GCATGAGGAG AAGCAGGACG AGCACGGATA TGTCTCCCGC 
CAGTTCTCCA GACGCTACCA GCTGCCAAGT GATGTCAACC CCGACACGGT CACATCGTCC 
CTCTCCTCCG ATGGCCTGCT GACCATCAAG GCGCCAATGA AGGCGCTGCC TCCTCCGCAG 
ACTGAACGCC TTGTGCAGAT CACACAGACT GGTCCCTCCT CCAAGGAGGA CAACGCCAAG 
AAGGTGGAGA CCTCCACCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(2)efl ( NM_079103.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523827.1 Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11072
Clone ID Related Accession (Same CDS sequence)	NM_001274227.1 , NM_079103.3
Accession Version	NM_079103.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) essential for life, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079103.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGTAG TGCCACTGAT GTTCCGTGAC TGGTGGGATG AGCTCGACTT TCCAATGCGC 
ACCTCGCGCC TCTTGGACCA GCACTTCGGT CAGGGACTGA AGAGGGATGA CCTCATGTCC 
TCCGTGTGGA ACTCCCGCCC CACAGTGCTG CGATCGGGAT ACCTGCGCCC CTGGCACACG 
AACAGCCTGC AGAAGCAGGA GTCCGGTTCC ACCCTCAACA TCGACAGCGA GAAGTTCGAG 
GTCATTCTGG ATGTGCAGCA GTTCTCGCCA TCCGAGATCA CCGTCAAGGT GGCCGACAAG 
TTCGTCATCG TGGAGGGAAA GCATGAGGAG AAGCAGGACG AGCACGGATA TGTCTCCCGC 
CAGTTCTCCA GACGCTACCA GCTGCCAAGT GATGTCAACC CCGACACGGT CACATCGTCC 
CTCTCCTCCG ATGGCCTGCT GACCATCAAG GCGCCAATGA AGGCGCTGCC TCCTCCGCAG 
ACTGAACGCC TTGTGCAGAT CACACAGACT GGTCCCTCCT CCAAGGAGGA CAACGCCAAG 
AAGGTGGAGA CCTCCACCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523827.1
CDS	69..632
Translation	MSVVPLMFRDWWDELDFPMRTSRLLDQHFGQGLKRDDLMSSVWNSRPTVLRSGYLRPWHTNSLQKQESGSTLNIDSEKFEVILDVQQFSPSEITVKVADKFVIVEGKHEEKQDEHGYVSRQFSRRYQLPSDVNPDTVTSSLSSDGLLTIKAPMKALPPPQTERLVQITQTGPSSKEDNAKKVETSTA

Target ORF information:

RefSeq Version	NM_079103.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) essential for life (l(2)efl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079103.3

ORF Insert Sequence:

ATGTCCGTAG TGCCACTGAT GTTCCGTGAC TGGTGGGATG AGCTCGACTT TCCAATGCGC 
ACCTCGCGCC TCTTGGACCA GCACTTCGGT CAGGGACTGA AGAGGGATGA CCTCATGTCC 
TCCGTGTGGA ACTCCCGCCC CACAGTGCTG CGATCGGGAT ACCTGCGCCC CTGGCACACG 
AACAGCCTGC AGAAGCAGGA GTCCGGTTCC ACCCTCAACA TCGACAGCGA GAAGTTCGAG 
GTCATTCTGG ATGTGCAGCA GTTCTCGCCA TCCGAGATCA CCGTCAAGGT GGCCGACAAG 
TTCGTCATCG TGGAGGGAAA GCATGAGGAG AAGCAGGACG AGCACGGATA TGTCTCCCGC 
CAGTTCTCCA GACGCTACCA GCTGCCAAGT GATGTCAACC CCGACACGGT CACATCGTCC 
CTCTCCTCCG ATGGCCTGCT GACCATCAAG GCGCCAATGA AGGCGCTGCC TCCTCCGCAG 
ACTGAACGCC TTGTGCAGAT CACACAGACT GGTCCCTCCT CCAAGGAGGA CAACGCCAAG 
AAGGTGGAGA CCTCCACCGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(2)efl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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