Tnp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnp1
Entrez Gene ID	24839
Full Name	transition protein 1
General protein information	Preferred Names transition protein 1 Names spermatid nuclear transition protein 1 STP-1 TP-1 testis-specific basic protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33
Summary	spermatid transitional protein; highly basic protein expressed during the brief period when histones are being replaced by protamines in the haploid stage of spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017056.2	NP_058752.1	spermatid nuclear transition protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnp1	NP_033433.1
Rattus norvegicus (Norway rat)	Tnp1	NP_058752.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Cout? Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, H?ry P, Fernandez-Nunez N, Shiota H, G?rard M, Rousseaux S, Kurumizaka H, Khochbin S
Molecular cell66(1)89-101.e8(2017 Apr)

Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.
Kolthur-Seetharam U, Pradeepa MM, Gupta N, Narayanaswamy R, Rao MR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(10)951-62(2009 Oct)

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
Govin J, Escoffier E, Rousseaux S, Kuhn L, Ferro M, Th?venon J, Catena R, Davidson I, Garin J, Khochbin S, Caron C
The Journal of cell biology176(3)283-94(2007 Jan)

Abnormalities and reduced reproductive potential of sperm from Tnp1- and Tnp2-null double mutant mice.
Shirley CR, Hayashi S, Mounsey S, Yanagimachi R, Meistrich ML
Biology of reproduction71(4)1220-9(2004 Oct)

Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations.
Zhao M, Shirley CR, Mounsey S, Meistrich ML
Biology of reproduction71(3)1016-25(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TP1 and TP2 may have concerted functional roles during chromatin remodeling in mammalian spermiogenesis.
Title: Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.

Results suggest that Sertoli cell-derived androgen-binding protein acts on spermatids by modifying the transition protein-1 mRNA level.
Title: Effects of androgen-binding protein (ABP) on spermatid Tnp1 gene expression in vitro.

The following Tnp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02638	NM_017056.2 Latest version!	Rattus norvegicus transition protein 1 (Tnp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnp1 ( NM_017056.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058752.1 Rattus norvegicus transition protein 1 (Tnp1), mRNA.

Price & Availability↑

CloneID	ORa02638
Clone ID Related Accession (Same CDS sequence)	NM_017056.2
Accession Version	NM_017056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	spermatid nuclear transition protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078850.1. On Aug 23, 2004 this sequence version replaced NM_017056.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078850.1, M17096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604 [ECO:0000348] ##Evidence-Data-END##

1
61
121

ATGTCGACCA GCCGCAAACT AAAGACTCAT GGCATGAGGA GAGGCAAGAA CCGAGCTCCT 
CACAAGGGCG TCAAGAGAGG AGGAAGCAAG AGAAAATACC GGAAGAGCAG CCTGAAGAGT 
AGGAAACGGG GCGATGATGC AAGTCGCAAT TACCGATCCC ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058752.1
CDS	18..185
Translation	MSTSRKLKTHGMRRGKNRAPHKGVKRGGSKRKYRKSSLKSRKRGDDASRNYRSHL

Target ORF information:

RefSeq Version	NM_017056.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transition protein 1 (Tnp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017056.2

ORF Insert Sequence:

1
61
121

ATGTCGACCA GCCGCAAACT AAAGACTCAT GGCATGAGGA GAGGCAAGAA CCGAGCTCCT 
CACAAGGGCG TCAAGAGAGG AGGAAGCAAG AGAAAATACC GGAAGAGCAG CCTGAAGAGT 
AGGAAACGGG GCGATGATGC AAGTCGCAAT TACCGATCCC ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Molecular cell66(1)89-101.e8(2017 Apr)
Barral S,Morozumi Y,Tanaka H,Montellier E,Govin J,de Dieuleveult M,Charbonnier G,Cout? Y,Puthier D,Buchou T,Boussouar F,Urahama T,Fenaille F,Curtet S,H?ry P,Fernandez-Nunez N,Shiota H,G?rard M,Rousseaux S,Kurumizaka H,Khochbin S

Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(10)951-62(2009 Oct)
Kolthur-Seetharam U,Pradeepa MM,Gupta N,Narayanaswamy R,Rao MR

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
The Journal of cell biology176(3)283-94(2007 Jan)
Govin J,Escoffier E,Rousseaux S,Kuhn L,Ferro M,Th?venon J,Catena R,Davidson I,Garin J,Khochbin S,Caron C

Abnormalities and reduced reproductive potential of sperm from Tnp1- and Tnp2-null double mutant mice.
Biology of reproduction71(4)1220-9(2004 Oct)
Shirley CR,Hayashi S,Mounsey S,Yanagimachi R,Meistrich ML

Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations.
Biology of reproduction71(3)1016-25(2004 Sep)
Zhao M,Shirley CR,Mounsey S,Meistrich ML

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Tnp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報