Ift172 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift172
Entrez Gene ID	116475
Full Name	intraflagellar transport 172
Synonyms	Slb
General protein information	Preferred Names intraflagellar transport 172 Names intraflagellar transport protein 172 homolog intraflagellar transport 172 homolog selective LIM binding factor homolog selective LIM binding factor, rat homolog selective LIM-binding factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	selectively interacts with Lhx3 and Lhx4; may be involved in modulating the transcriptional activity of specific LIM factors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053792.1	NP_446244.1	intraflagellar transport protein 172 homolog

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	IFT172	XP_419993.2
Drosophila melanogaster (fruit fly)	osm-1	NP_647700.2
	IFT172	XP_001097501.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005330	XP_555970.1
Bos taurus (cattle)	IFT172	XP_003586706.1
Xenopus tropicalis (tropical clawed frog)	LOC100490796	XP_002943869.2
Pan troglodytes (chimpanzee)	IFT172	XP_003308981.1
Mus musculus (house mouse)	Ift172	NP_080574.5
Rattus norvegicus (Norway rat)	Ift172	NP_446244.1
Homo sapiens (human)	IFT172	NP_056477.1
Canis lupus familiaris (dog)	IFT172	XP_540128.2
Danio rerio (zebrafish)	ift172	NP_001002312.1
Caenorhabditis elegans (roundworm)	osm-1	NP_510681.4

Gene Ontology
Bibliography

Related articles in PubMed

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV
Developmental cell31(3)265-78(2014 Nov)

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jim?nez CR
Journal of proteomics106191-204(2014 Jun)

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
Ishikawa H, Ide T, Yagi T, Jiang X, Hirono M, Sasaki H, Yanagisawa H, Wemmer KA, Stainier DY, Qin H, Kamiya R, Marshall WF
eLife3e01566(2014 Jan)

Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans.
Halbritter J, Bizet AA, Schmidts M, Porath JD, Braun DA, Gee HY, McInerney-Leo AM, Krug P, Filhol E, Davis EE, Airik R, Czarnecki PG, Lehman AM, Trnka P, Nitschk? P, Bole-Feysot C, Schueler M, Knebelmann B, Burtey S, Szab? AJ, Tory K, Leo PJ, Gardiner B, McKenzie FA, Zankl A, Brown MA, Hartley JL, Maher ER, Li C, Leroux MR, Scambler PJ, Zhan SH, Jones SJ, Kayserili H, Tuysuz B, Moorani KN, Constantinescu A, Krantz ID, Kaplan BS, Shah JV, , Hurd TW, Doherty D, Katsanis N, Duncan EL, Otto EA, Beales PL, Mitchison HM, Saunier S, Hildebrandt F
American journal of human genetics93(5)915-25(2013 Nov)

CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending.
Kr?hling AM, Alvarez L, Debowski K, Van Q, Gunkel M, Irsen S, Al-Amoudi A, Str?nker T, Kremmer E, Krause E, Voigt I, W?rtge S, Waisman A, Weyand I, Seifert R, Kaupp UB, Wachten D
PLoS genetics9(12)e1003960(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ift172 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift172 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10658	NM_053792.1 Latest version!	Rattus norvegicus intraflagellar transport 172 (Ift172), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift172 ( NM_053792.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446244.1 Rattus norvegicus intraflagellar transport 172 (Ift172), mRNA.

Price & Availability↑

CloneID	ORa10658
Clone ID Related Accession (Same CDS sequence)	NM_053792.1
Accession Version	NM_053792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 172 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF226993.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF226993.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTTGA AGCACCTGAG GACCCTGCTG AGCCCTCAGG ATGGAGCTGC GAAGGTGACC 
TGCATGGCCT GGTCCCAGAA CAATGCTAAA TTTGCTGTCT GCACAGTGGA CCGAGTGGTA 
CTGCTGTACG ATGAACACGG GGAGCGGAGA GACAAGTTCT CCACCAAACC AGCTGACATG 
AAGTATGGCA GGAAGAGCTA CATGGTCAAA GGCATGGCTT TCTCTCCTGA TTCCACAAAA 
ATTGCCATAG GACAGACTGA CAACATCATC TATGTCTACA AGATAGGAGA AGACTGGGGT 
GACAAGAAGG TCATCTGCAA CAAGTTCATC CAGACGAGTG CCGTCACTTG TCTGCAGTGG 
CCTGCGGAGT ATATCATTGT CTTTGGACTG GCTGAAGGCA AGGTTCGCTT AGCAAACACA 
AAAACTAACA AGTCATCTAC TATCTATGGG ACAGATTCCT ACGTGGTAGC GCTGACAACC 
AACTGCTCTG GGAAAGGGAT TCTCTCGGGT CACGCGGACG GCACCATCGT TAGGTATTTC 
TTCGATGACG AAGGCTCGGG AGAGTCACAG GGGAAGTTGG TTAACCATCC ATGCCCACCC 
TATGCCTTGG CCTGGGCCAC CAACAGCATT GTGGCTGCTG GCTGTGATCG GAGGATTGTG 
GCCTATGGAA AGGAAGGCCA TGTGCTACAG ACTTTCGACT ATAGCCGTGA CCCTCAGGAG 
CGGGAGTTCA CCACAGCTGC TGCAAGTCCT GGAGGCCAGT CTGTTGTTCT TGGAAGTTAT 
GACAGACTTC GGGTGTTCAA CTGGAGTCCT CGAAGAAGCA TCTGGGAAGA GGCAAAGCCC 
AAGGAGATTG CCAACTTATA CACAGTCACC GCCTTGGCCT GGAAGCGGGA TGGCTCTCGG 
CTCTGTGCGG GAACACTCTG TGGTGGGGTG GAACAGTTTG ACTGCTGCCT CCGAAGGAGC 
ATTTACAAGA ACAAGTTCGA GCTGACATAT GTGGGCCCTA GCCAGCTGAT TGTGAAGAAC 
CTGTCTTCGG GGACCAGGGT GGTGCTCAAG TCACACTATG GCTACGAAGT GGAAGAGGTG 
AAAATCCTGG GAAAGGAACG TTACCTGGTG GCTCACACTT CAGACACGCT GCTGCTGGGA 
GACCTCAACA CAAACCGCCT CAGTGAGATA CCCTGGCAGG GATCGGGTGG CAATGAGAAA 
TATTTCTTTG AAAATGAGAA TGTCTGCATG ATCTTCAATG CTGGAGAGCT GACCCTGGTG 
GAATATGGGA GTAACGACTC CTTGGGTTCT GTGCGCACTG AATTCATGAA CCCTCACCTC 
ATCAGCGTCC GTATCAATGA GAGGTGCCAG CGAGGAATGG AAGACAATAA GAAATTGGCT 
TATCTTGTTG ATATTAAGAC GATTGCTATA GTGGATCTGA TCGGTGGCTA CAACATTGGT 
ACTATCAGTC ACGAAAGCCG TGTGGATTGG CTGGAACTTA ACGAAACTGG GCACAAGCTT 
CTCTTCAGAG ACCGGAAACT TCGTTTGCAT CTGTACGATA TTGAAAGCTG CTCTAAGACA 
ATGATCCTGA ACTTCTGCTC CTACGTGCAG TGGGTTCCCG GGAGTGATGT GCTGGTGGCT 
CAGAACCGGA ACAGCTTATG TGTCTGGTAC AATATCGAGG CACCTGAGAG AGTCACCATG 
TCCTCTATCA GGGGTGATGT TGTCGGTCTG GAGAGAGGTG GTGGGAAGAC GGAGGTCATG 
GTGACAGAAG GTGTGACTAC TGTGGCCTAC ACCCTGGATG AGGGCCTGAT TGAGTTTGGA 
ACAGCCATCG ATGACGGCAA CTACACCCGG GCAACAGCCT TCTTGGAGAC CCTGGAGATG 
ACCCCAGAAA CAGAGGCAAT GTGGAAAACC TTGAGCAAAC TGGCCCTGGA AGCAAGGCAG 
CTTCACACTG CTGAGAGGTG CTTCTCTGCT TTGGGTCACG TGGCCAAAGC TCGGTTCCTG 
CATGAGACCA ATGAGATAGC GGATCAGGTG TCCAGGGAAT ATGGTGGAGA AGGAACGGAC 
TTCTATCAGG TTCGAGCTCG CCTCGCCATG CTGGAGAAGA ACTACAAACT GGCCGAAATG 
ATCTTCTTGG AACAGAATGC TGTTGAAGAA GCCATGGACA TGTACCAGGA GCTGCACCGC 
TGGGATGAAT GCATTGCTGT GGCTGAGGCC AAGGGACACC CAGCCCTGGA GAAGCTACGC 
AGAGATTACT ATCAGAGATT AATGGACACA CAGCAAGAGG AACGAGCAGG TGAACTGCAG 
GAGAGTCAAG GGGATGGGCT GGCAGCCATC AGCCTCTACC TCAAAGCTGG GCTCCCTGCC 
AAAGCCGCAC GGCTGGTGCT GACGCGAGAG GAGCTGCTGG CCAACACGGA GCTGGTAGAA 
CATATCACCA CAGCCCTCAT CAAGGGAGAG CTCTATGAAA GGGCAGGTGA TCTCTTTGAG 
AAGATTCGAA ATCCACAGCG GGCCCTTGAG TGCTACTGTA AAGGCAACGC ATTCATGAAA 
GCTGTTGAGC TGGCTCGACT GGCCTTCCCA GTGGAGGTGG TGAGGCTGGA GGAGGCCTGG 
GGGGACCACT TGGTACAGCA AAAGCAGCTG GACGCGGCCA TCAATCACTA TATTGAAGCC 
AGGTGCTCCA TTAAAGCAAT TGAGGCTGCC CTGGGTGCCC GCCAGTGGAA GAAGGCAATT 
TATATATTAG ATCTGCAGGA TAGAAACACT GCATCTAAAT ACTATCCTCG TGTGGCCCAA 
CACTATGCAT CTCTGCAGGA GTATGAGATC GCTGAGGAGC TCTACACCAA AGGAGACCGG 
ACCAAAGATG CTATAGACAT GTACACCCAG GCTGGTCGCT GGGAGCAAGC CCACAAGCTG 
GCCATGAAGT GCATGCGACC AGAAGACGTG TCAGTGCTCT ACATCACACA GGCCCAGGAA 
ATGGAGAAGC AGGGCAAGTA CCGAGAGGCC GAGAGGCTGT ACGTGACAGT GGAGGAGCCT 
GATCTTGCCA TCACCATGTT CAAAAAGCAC AAGTTGTATG ACGACATGAT TCGCCTGGTG 
GGGAAGCACC ATCCAGACCT CCTCAGCGAC ACACACCTTC ACCTGGGCAA GGAGCTGGAG 
ACAGAAGGCC GACTGCAAGA GGCTGAGTAC CACTACCTCG AGGCCCAGGA ATGGAAGGCA 
ACTGTGAACA TGTACCGGTC CAGCGGGCTT TGGGAGGAGG CCTACCGGGT GGCCAAAGCC 
CACGGAGGAG CCAACGCCCA CAAACACGTG GCCTATCTGT GGGCGAAGAG CCTGGGAGGA 
GAGGCTGCGG TCAGACTGCT CAACAAGCTG GGGCTTCTAG AGGCTGCCAT TGACCATGCT 
GCTGACAACT GCTCCTTTGA GTTTGCCTTT GAACTCTCTC GGCTAGCCCT CAAGCACAAA 
ACCCCGGAGA TTCATCTCAG ATACGCCATG TACCTGGAAG ATGAGGGTAA ATTTGAAGAG 
GCTGAAGCTG AATTCATCCG GGCTGGTAAA CCCAAGGAGG CAGTCCTCAT GTTTGTCCAC 
AACCAGGATT GGGAGGCAGC TCAGCGTGTG GCTGAGGCCC ACGATCCTGA CAGTGTTGCT 
GAGGTACTTG TAGGCCAGGC CCGTGGGGCC TTGGAGGAGA AGGACTTTCA GAAGGCAGAA 
GGGTTGCTGC TTCGGGCCCA GAGACCAGGC CTGGCTCTCA ATTATTATAA GGAGGCTGGA 
CTCTGGAGTG ATGCTCTGAG GATCTGCAAG GACTATGTGC CTGGCCAGCT GGAGGCCCTG 
CAGGAAGAGT ATGAGCGAGA AGCTACCAAG AAGGGGGGCA GAGGCGTGGA GGGACTTGTA 
GAACAGGCAC GGCAATGGGA ACAGGCTGGA GAGTATAGCC GTGCAGTGGA CTGCTACCTC 
AAAGTTCGAG ACTCAGGAAG CAGTGGCCTG ATGGAGAAGT GCTGGATGAA GGCGGCTGAA 
CTGTCCATCA AGTTCCTGCC TCCCCAGCGA AGTCTGGAAG TGGTTCGGGT TGTAGGTCCC 
CAGCTGATTG GAATTGGAAA GCACAGTGCT TCTGCAGAAC TCTATCTGAA CCTGGACCTT 
GTCAAAGAAG CAATTGATGC CTTCATTGAG GGTGAAGAGT GGAACAAGGC CAAGCGCGTG 
GCCAAAGAGC TAGATCCCCG GTATGAAGAC TATGTGGATC AGCATTATAA GGAGTTCCTC 
AAGAACCAGG GTAAAGTGGA TTCGCTGGTG GGTGTGGACG TAGTAGCTGC CTTGGACCTG 
TATGTGGAGC AGGGCCAGTG GGATAAGTGC ATTGAAACGG CTACCAAACA GAACTACAAG 
ATTCTGCACA AGTATGTGGC TCTGTATGCG ACTCATCTGA TCCGAGAAGG TGGCTATGCC 
CAGGCCCTGG CCCTCTACGT GCAACATGGA GCCCCTGCTA ACCCCCAGAA TTTCAACATC 
TACAAAAGGA TCTTCACTGA CATGGTTAGC TCTCCTGGGA CCAACAATGC TGAGGCCTAC 
CATAGCTGGG CTGACCTTCG GGATGTCCTC TTCAACCTGT GTGAAAACCT GGTGAAGTCC 
AGCGAGGCAA ACTCGGCAGC TCATGAGGAG TTTGAGATGA TGCTGCTGAT CGCTCATTAC 
TATGCGACAC GATCCGCAGC GCAGAGCATC AAACAGCTGG AAACCGTAGC TGCTAGACTT 
TCCGTTTCGC TCTTGCGCCA CACCCAGTTA CTACCTGCGG ACAAGGCCTT TTATGAAGCA 
GGCACTGCTG CCAAGGAAGT TGGCTGGGAG AACATGGCCT TCATTTTCTT CAACCGATTT 
TTGGATCTGA CTGATGCCAT CGAAGAAGGG ACCTTGGATG CCCTGGACCA CTCTGATTTT 
CAGGACACAG ACATTCCCTT TGAGGTGCCA CTCCCTGCCA AGCAGCATGT CCCGGAGGCC 
CAGAGAGAAG AGGTTCGAGA CTGGGTGCTC ACAGTCTCGA TGGACCAGAG GCTGGAGCAG 
GTTCTGCCCC GAGATGAGCG TGGTGTCTAC GAGGCTTCCC TAGTGGCAGC GAGCACTGGA 
GTCCGGGCCC TGCCCTGTCT CATTACAGGT TACCCCATTC TGAGGAACAA AATTGAATTT 
AAGCGGCCTG GGAAGGCTGC TAACAAAGAC AACTGGAACA AGTTTCTTAT GGCCATCAAG 
ACCTCCCACA GTCCAGTGTG CCAGGATGTG CTCAAATTCA TCAGCCAGTG GTGTGGTGGG 
CTCCCCAGCA CCAGCTTTTC CTTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446244.1
CDS	89..5338
Translation	MQLKHLRTLLSPQDGAAKVTCMAWSQNNAKFAVCTVDRVVLLYDEHGERRDKFSTKPADMKYGRKSYMVKGMAFSPDSTKIAIGQTDNIIYVYKIGEDWGDKKVICNKFIQTSAVTCLQWPAEYIIVFGLAEGKVRLANTKTNKSSTIYGTDSYVVALTTNCSGKGILSGHADGTIVRYFFDDEGSGESQGKLVNHPCPPYALAWATNSIVAAGCDRRIVAYGKEGHVLQTFDYSRDPQEREFTTAAASPGGQSVVLGSYDRLRVFNWSPRRSIWEEAKPKEIANLYTVTALAWKRDGSRLCAGTLCGGVEQFDCCLRRSIYKNKFELTYVGPSQLIVKNLSSGTRVVLKSHYGYEVEEVKILGKERYLVAHTSDTLLLGDLNTNRLSEIPWQGSGGNEKYFFENENVCMIFNAGELTLVEYGSNDSLGSVRTEFMNPHLISVRINERCQRGMEDNKKLAYLVDIKTIAIVDLIGGYNIGTISHESRVDWLELNETGHKLLFRDRKLRLHLYDIESCSKTMILNFCSYVQWVPGSDVLVAQNRNSLCVWYNIEAPERVTMSSIRGDVVGLERGGGKTEVMVTEGVTTVAYTLDEGLIEFGTAIDDGNYTRATAFLETLEMTPETEAMWKTLSKLALEARQLHTAERCFSALGHVAKARFLHETNEIADQVSREYGGEGTDFYQVRARLAMLEKNYKLAEMIFLEQNAVEEAMDMYQELHRWDECIAVAEAKGHPALEKLRRDYYQRLMDTQQEERAGELQESQGDGLAAISLYLKAGLPAKAARLVLTREELLANTELVEHITTALIKGELYERAGDLFEKIRNPQRALECYCKGNAFMKAVELARLAFPVEVVRLEEAWGDHLVQQKQLDAAINHYIEARCSIKAIEAALGARQWKKAIYILDLQDRNTASKYYPRVAQHYASLQEYEIAEELYTKGDRTKDAIDMYTQAGRWEQAHKLAMKCMRPEDVSVLYITQAQEMEKQGKYREAERLYVTVEEPDLAITMFKKHKLYDDMIRLVGKHHPDLLSDTHLHLGKELETEGRLQEAEYHYLEAQEWKATVNMYRSSGLWEEAYRVAKAHGGANAHKHVAYLWAKSLGGEAAVRLLNKLGLLEAAIDHAADNCSFEFAFELSRLALKHKTPEIHLRYAMYLEDEGKFEEAEAEFIRAGKPKEAVLMFVHNQDWEAAQRVAEAHDPDSVAEVLVGQARGALEEKDFQKAEGLLLRAQRPGLALNYYKEAGLWSDALRICKDYVPGQLEALQEEYEREATKKGGRGVEGLVEQARQWEQAGEYSRAVDCYLKVRDSGSSGLMEKCWMKAAELSIKFLPPQRSLEVVRVVGPQLIGIGKHSASAELYLNLDLVKEAIDAFIEGEEWNKAKRVAKELDPRYEDYVDQHYKEFLKNQGKVDSLVGVDVVAALDLYVEQGQWDKCIETATKQNYKILHKYVALYATHLIREGGYAQALALYVQHGAPANPQNFNIYKRIFTDMVSSPGTNNAEAYHSWADLRDVLFNLCENLVKSSEANSAAHEEFEMMLLIAHYYATRSAAQSIKQLETVAARLSVSLLRHTQLLPADKAFYEAGTAAKEVGWENMAFIFFNRFLDLTDAIEEGTLDALDHSDFQDTDIPFEVPLPAKQHVPEAQREEVRDWVLTVSMDQRLEQVLPRDERGVYEASLVAASTGVRALPCLITGYPILRNKIEFKRPGKAANKDNWNKFLMAIKTSHSPVCQDVLKFISQWCGGLPSTSFSFQ

Target ORF information:

RefSeq Version	NM_053792.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 172 (Ift172), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053792.1

ORF Insert Sequence:

ATGCAGTTGA AGCACCTGAG GACCCTGCTG AGCCCTCAGG ATGGAGCTGC GAAGGTGACC 
TGCATGGCCT GGTCCCAGAA CAATGCTAAA TTTGCTGTCT GCACAGTGGA CCGAGTGGTA 
CTGCTGTACG ATGAACACGG GGAGCGGAGA GACAAGTTCT CCACCAAACC AGCTGACATG 
AAGTATGGCA GGAAGAGCTA CATGGTCAAA GGCATGGCTT TCTCTCCTGA TTCCACAAAA 
ATTGCCATAG GACAGACTGA CAACATCATC TATGTCTACA AGATAGGAGA AGACTGGGGT 
GACAAGAAGG TCATCTGCAA CAAGTTCATC CAGACGAGTG CCGTCACTTG TCTGCAGTGG 
CCTGCGGAGT ATATCATTGT CTTTGGACTG GCTGAAGGCA AGGTTCGCTT AGCAAACACA 
AAAACTAACA AGTCATCTAC TATCTATGGG ACAGATTCCT ACGTGGTAGC GCTGACAACC 
AACTGCTCTG GGAAAGGGAT TCTCTCGGGT CACGCGGACG GCACCATCGT TAGGTATTTC 
TTCGATGACG AAGGCTCGGG AGAGTCACAG GGGAAGTTGG TTAACCATCC ATGCCCACCC 
TATGCCTTGG CCTGGGCCAC CAACAGCATT GTGGCTGCTG GCTGTGATCG GAGGATTGTG 
GCCTATGGAA AGGAAGGCCA TGTGCTACAG ACTTTCGACT ATAGCCGTGA CCCTCAGGAG 
CGGGAGTTCA CCACAGCTGC TGCAAGTCCT GGAGGCCAGT CTGTTGTTCT TGGAAGTTAT 
GACAGACTTC GGGTGTTCAA CTGGAGTCCT CGAAGAAGCA TCTGGGAAGA GGCAAAGCCC 
AAGGAGATTG CCAACTTATA CACAGTCACC GCCTTGGCCT GGAAGCGGGA TGGCTCTCGG 
CTCTGTGCGG GAACACTCTG TGGTGGGGTG GAACAGTTTG ACTGCTGCCT CCGAAGGAGC 
ATTTACAAGA ACAAGTTCGA GCTGACATAT GTGGGCCCTA GCCAGCTGAT TGTGAAGAAC 
CTGTCTTCGG GGACCAGGGT GGTGCTCAAG TCACACTATG GCTACGAAGT GGAAGAGGTG 
AAAATCCTGG GAAAGGAACG TTACCTGGTG GCTCACACTT CAGACACGCT GCTGCTGGGA 
GACCTCAACA CAAACCGCCT CAGTGAGATA CCCTGGCAGG GATCGGGTGG CAATGAGAAA 
TATTTCTTTG AAAATGAGAA TGTCTGCATG ATCTTCAATG CTGGAGAGCT GACCCTGGTG 
GAATATGGGA GTAACGACTC CTTGGGTTCT GTGCGCACTG AATTCATGAA CCCTCACCTC 
ATCAGCGTCC GTATCAATGA GAGGTGCCAG CGAGGAATGG AAGACAATAA GAAATTGGCT 
TATCTTGTTG ATATTAAGAC GATTGCTATA GTGGATCTGA TCGGTGGCTA CAACATTGGT 
ACTATCAGTC ACGAAAGCCG TGTGGATTGG CTGGAACTTA ACGAAACTGG GCACAAGCTT 
CTCTTCAGAG ACCGGAAACT TCGTTTGCAT CTGTACGATA TTGAAAGCTG CTCTAAGACA 
ATGATCCTGA ACTTCTGCTC CTACGTGCAG TGGGTTCCCG GGAGTGATGT GCTGGTGGCT 
CAGAACCGGA ACAGCTTATG TGTCTGGTAC AATATCGAGG CACCTGAGAG AGTCACCATG 
TCCTCTATCA GGGGTGATGT TGTCGGTCTG GAGAGAGGTG GTGGGAAGAC GGAGGTCATG 
GTGACAGAAG GTGTGACTAC TGTGGCCTAC ACCCTGGATG AGGGCCTGAT TGAGTTTGGA 
ACAGCCATCG ATGACGGCAA CTACACCCGG GCAACAGCCT TCTTGGAGAC CCTGGAGATG 
ACCCCAGAAA CAGAGGCAAT GTGGAAAACC TTGAGCAAAC TGGCCCTGGA AGCAAGGCAG 
CTTCACACTG CTGAGAGGTG CTTCTCTGCT TTGGGTCACG TGGCCAAAGC TCGGTTCCTG 
CATGAGACCA ATGAGATAGC GGATCAGGTG TCCAGGGAAT ATGGTGGAGA AGGAACGGAC 
TTCTATCAGG TTCGAGCTCG CCTCGCCATG CTGGAGAAGA ACTACAAACT GGCCGAAATG 
ATCTTCTTGG AACAGAATGC TGTTGAAGAA GCCATGGACA TGTACCAGGA GCTGCACCGC 
TGGGATGAAT GCATTGCTGT GGCTGAGGCC AAGGGACACC CAGCCCTGGA GAAGCTACGC 
AGAGATTACT ATCAGAGATT AATGGACACA CAGCAAGAGG AACGAGCAGG TGAACTGCAG 
GAGAGTCAAG GGGATGGGCT GGCAGCCATC AGCCTCTACC TCAAAGCTGG GCTCCCTGCC 
AAAGCCGCAC GGCTGGTGCT GACGCGAGAG GAGCTGCTGG CCAACACGGA GCTGGTAGAA 
CATATCACCA CAGCCCTCAT CAAGGGAGAG CTCTATGAAA GGGCAGGTGA TCTCTTTGAG 
AAGATTCGAA ATCCACAGCG GGCCCTTGAG TGCTACTGTA AAGGCAACGC ATTCATGAAA 
GCTGTTGAGC TGGCTCGACT GGCCTTCCCA GTGGAGGTGG TGAGGCTGGA GGAGGCCTGG 
GGGGACCACT TGGTACAGCA AAAGCAGCTG GACGCGGCCA TCAATCACTA TATTGAAGCC 
AGGTGCTCCA TTAAAGCAAT TGAGGCTGCC CTGGGTGCCC GCCAGTGGAA GAAGGCAATT 
TATATATTAG ATCTGCAGGA TAGAAACACT GCATCTAAAT ACTATCCTCG TGTGGCCCAA 
CACTATGCAT CTCTGCAGGA GTATGAGATC GCTGAGGAGC TCTACACCAA AGGAGACCGG 
ACCAAAGATG CTATAGACAT GTACACCCAG GCTGGTCGCT GGGAGCAAGC CCACAAGCTG 
GCCATGAAGT GCATGCGACC AGAAGACGTG TCAGTGCTCT ACATCACACA GGCCCAGGAA 
ATGGAGAAGC AGGGCAAGTA CCGAGAGGCC GAGAGGCTGT ACGTGACAGT GGAGGAGCCT 
GATCTTGCCA TCACCATGTT CAAAAAGCAC AAGTTGTATG ACGACATGAT TCGCCTGGTG 
GGGAAGCACC ATCCAGACCT CCTCAGCGAC ACACACCTTC ACCTGGGCAA GGAGCTGGAG 
ACAGAAGGCC GACTGCAAGA GGCTGAGTAC CACTACCTCG AGGCCCAGGA ATGGAAGGCA 
ACTGTGAACA TGTACCGGTC CAGCGGGCTT TGGGAGGAGG CCTACCGGGT GGCCAAAGCC 
CACGGAGGAG CCAACGCCCA CAAACACGTG GCCTATCTGT GGGCGAAGAG CCTGGGAGGA 
GAGGCTGCGG TCAGACTGCT CAACAAGCTG GGGCTTCTAG AGGCTGCCAT TGACCATGCT 
GCTGACAACT GCTCCTTTGA GTTTGCCTTT GAACTCTCTC GGCTAGCCCT CAAGCACAAA 
ACCCCGGAGA TTCATCTCAG ATACGCCATG TACCTGGAAG ATGAGGGTAA ATTTGAAGAG 
GCTGAAGCTG AATTCATCCG GGCTGGTAAA CCCAAGGAGG CAGTCCTCAT GTTTGTCCAC 
AACCAGGATT GGGAGGCAGC TCAGCGTGTG GCTGAGGCCC ACGATCCTGA CAGTGTTGCT 
GAGGTACTTG TAGGCCAGGC CCGTGGGGCC TTGGAGGAGA AGGACTTTCA GAAGGCAGAA 
GGGTTGCTGC TTCGGGCCCA GAGACCAGGC CTGGCTCTCA ATTATTATAA GGAGGCTGGA 
CTCTGGAGTG ATGCTCTGAG GATCTGCAAG GACTATGTGC CTGGCCAGCT GGAGGCCCTG 
CAGGAAGAGT ATGAGCGAGA AGCTACCAAG AAGGGGGGCA GAGGCGTGGA GGGACTTGTA 
GAACAGGCAC GGCAATGGGA ACAGGCTGGA GAGTATAGCC GTGCAGTGGA CTGCTACCTC 
AAAGTTCGAG ACTCAGGAAG CAGTGGCCTG ATGGAGAAGT GCTGGATGAA GGCGGCTGAA 
CTGTCCATCA AGTTCCTGCC TCCCCAGCGA AGTCTGGAAG TGGTTCGGGT TGTAGGTCCC 
CAGCTGATTG GAATTGGAAA GCACAGTGCT TCTGCAGAAC TCTATCTGAA CCTGGACCTT 
GTCAAAGAAG CAATTGATGC CTTCATTGAG GGTGAAGAGT GGAACAAGGC CAAGCGCGTG 
GCCAAAGAGC TAGATCCCCG GTATGAAGAC TATGTGGATC AGCATTATAA GGAGTTCCTC 
AAGAACCAGG GTAAAGTGGA TTCGCTGGTG GGTGTGGACG TAGTAGCTGC CTTGGACCTG 
TATGTGGAGC AGGGCCAGTG GGATAAGTGC ATTGAAACGG CTACCAAACA GAACTACAAG 
ATTCTGCACA AGTATGTGGC TCTGTATGCG ACTCATCTGA TCCGAGAAGG TGGCTATGCC 
CAGGCCCTGG CCCTCTACGT GCAACATGGA GCCCCTGCTA ACCCCCAGAA TTTCAACATC 
TACAAAAGGA TCTTCACTGA CATGGTTAGC TCTCCTGGGA CCAACAATGC TGAGGCCTAC 
CATAGCTGGG CTGACCTTCG GGATGTCCTC TTCAACCTGT GTGAAAACCT GGTGAAGTCC 
AGCGAGGCAA ACTCGGCAGC TCATGAGGAG TTTGAGATGA TGCTGCTGAT CGCTCATTAC 
TATGCGACAC GATCCGCAGC GCAGAGCATC AAACAGCTGG AAACCGTAGC TGCTAGACTT 
TCCGTTTCGC TCTTGCGCCA CACCCAGTTA CTACCTGCGG ACAAGGCCTT TTATGAAGCA 
GGCACTGCTG CCAAGGAAGT TGGCTGGGAG AACATGGCCT TCATTTTCTT CAACCGATTT 
TTGGATCTGA CTGATGCCAT CGAAGAAGGG ACCTTGGATG CCCTGGACCA CTCTGATTTT 
CAGGACACAG ACATTCCCTT TGAGGTGCCA CTCCCTGCCA AGCAGCATGT CCCGGAGGCC 
CAGAGAGAAG AGGTTCGAGA CTGGGTGCTC ACAGTCTCGA TGGACCAGAG GCTGGAGCAG 
GTTCTGCCCC GAGATGAGCG TGGTGTCTAC GAGGCTTCCC TAGTGGCAGC GAGCACTGGA 
GTCCGGGCCC TGCCCTGTCT CATTACAGGT TACCCCATTC TGAGGAACAA AATTGAATTT 
AAGCGGCCTG GGAAGGCTGC TAACAAAGAC AACTGGAACA AGTTTCTTAT GGCCATCAAG 
ACCTCCCACA GTCCAGTGTG CCAGGATGTG CTCAAATTCA TCAGCCAGTG GTGTGGTGGG 
CTCCCCAGCA CCAGCTTTTC CTTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Developmental cell31(3)265-78(2014 Nov)
Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Journal of proteomics106191-204(2014 Jun)
Chiasserini D,van Weering JR,Piersma SR,Pham TV,Malekzadeh A,Teunissen CE,de Wit H,Jim?nez CR

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
eLife3e01566(2014 Jan)
Ishikawa H,Ide T,Yagi T,Jiang X,Hirono M,Sasaki H,Yanagisawa H,Wemmer KA,Stainier DY,Qin H,Kamiya R,Marshall WF

Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans.
American journal of human genetics93(5)915-25(2013 Nov)
Halbritter J,Bizet AA,Schmidts M,Porath JD,Braun DA,Gee HY,McInerney-Leo AM,Krug P,Filhol E,Davis EE,Airik R,Czarnecki PG,Lehman AM,Trnka P,Nitschk? P,Bole-Feysot C,Schueler M,Knebelmann B,Burtey S,Szab? AJ,Tory K,Leo PJ,Gardiner B,McKenzie FA,Zankl A,Brown MA,Hartley JL,Maher ER,Li C,Leroux MR,Scambler PJ,Zhan SH,Jones SJ,Kayserili H,Tuysuz B,Moorani KN,Constantinescu A,Krantz ID,Kaplan BS,Shah JV,,Hurd TW,Doherty D,Katsanis N,Duncan EL,Otto EA,Beales PL,Mitchison HM,Saunier S,Hildebrandt F

CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending.
PLoS genetics9(12)e1003960(2013)
Kr?hling AM,Alvarez L,Debowski K,Van Q,Gunkel M,Irsen S,Al-Amoudi A,Str?nker T,Kremmer E,Krause E,Voigt I,W?rtge S,Waisman A,Weyand I,Seifert R,Kaupp UB,Wachten D

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Ift172 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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