ift172 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ift172
Entrez Gene ID	432389
Full Name	intraflagellar transport 172
General protein information	Preferred Names intraflagellar transport 172 Names intraflagellar transport protein 172 homolog LIM-interacting protein moe
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002312.1	NP_001002312.1	intraflagellar transport protein 172 homolog

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	IFT172	XP_419993.2
Drosophila melanogaster (fruit fly)	osm-1	NP_647700.2
	IFT172	XP_001097501.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005330	XP_555970.1
Bos taurus (cattle)	IFT172	XP_003586706.1
Xenopus tropicalis (tropical clawed frog)	LOC100490796	XP_002943869.2
Pan troglodytes (chimpanzee)	IFT172	XP_003308981.1
Mus musculus (house mouse)	Ift172	NP_080574.5
Rattus norvegicus (Norway rat)	Ift172	NP_446244.1
Homo sapiens (human)	IFT172	NP_056477.1
Canis lupus familiaris (dog)	IFT172	XP_540128.2
Danio rerio (zebrafish)	ift172	NP_001002312.1
Caenorhabditis elegans (roundworm)	osm-1	NP_510681.4

Gene Ontology
Bibliography

Related articles in PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish IFT mutants also exhibited no dramatic changes in the craniofacial skeleton, somite formation, or motor neuron patterning.
Title: Zebrafish ift57, ift88, and ift172 intraflagellar transport mutants disrupt cilia but do not affect hedgehog signaling.

The following ift172 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ift172 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38487	NM_001002312.1 Latest version!	Danio rerio intraflagellar transport 172 (ift172), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ift172 ( NM_001002312.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002312.1 Danio rerio intraflagellar transport 172 (ift172), mRNA.

Price & Availability↑

CloneID	ODa38487
Clone ID Related Accession (Same CDS sequence)	NM_001002312.1
Accession Version	NM_001002312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	intraflagellar transport protein 172 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY618923.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY618923.1, BC163767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCTTA AGTACATGAA GACTCTTTTA ACGCCTCAGG ATGGAGCTGC TAAAGTCACC 
TGTATGGCTT GGGCTCCAAA CAATGCAAAG TTTGCAGTGT GCACTATTGA TAGAGTGGTT 
ATTTTGTATG ATGAGCAGGG AGAGAAAAGA GACAGATTTA CAACAAAGCC TGCTGACCCA 
AAATACGGAA AGAAAACCTA TGTGGTCAAG TCTATGGCAT TTTCCCCTGA TTCAACCAAG 
ATAGCAGTTG CTCAAACAGA CAACATCATA TTTGTTTACA AAATTGGAGA GGAGTGGGGA 
GATAAAAAGG TTATTTGCAA CAAGTTCATT CAAACAAGTG CTGTTACAAG CCTGGTTTGG 
CCGTCTGAGC ATGCAATTAT ATTTGGACTA GCAGAAGGAA AAGTTCGACA AGCTAATACA 
AAAACAAGCA AGTCCTCAAC AATCTATGGA ACAGACTCTT ATGTTGTCTC ACTAACCTCA 
AATGTATCAG GAAAGGGAAT CCTTTCCGGT CACGCAGATG GAACGGTGGT TCGGTATTTC 
ATTGATGATG AAGGATCTGG GGAATCTCAG GGCAAACTGC TGACTCATGC ATGTCCACCA 
TATGCCTTAG CATGGGGCAC AAACAGCATA ATTGTGGCTG GGTGCGATAA GAAGATTGTT 
GCCTATGGGA AGGAGGGCAA CGTGATTCAG ACATTTGATT ACAGCCGAGA TCGAGCCGAA 
AAGGAATTCA CTGTGGCTGC ATCGAGCCCT AGTGGCCAGT CCATAGTTGT AGGAAGCTTT 
GACAGATTGC GAGTTTTTAA TTGGAGTCCT CGAAAAGGCA CATGGGATGA ATCTTCTCCC 
AAAGAAATTC CAAACCTGTA CACCATCACA GCTCTCTCCT GGAAAAAAGA TGGCTCTCGG 
CTTTCTGTGG GCACTCTGTG TGGAGGTGTC GAGATGTTTG ACTGCTGTCT CCGACGGAGT 
ATTTACAAAA ACAAATTTGA GATGACATAC GTAGGACTAA GTCAGGTGAT TGTGAGGAAT 
TTGTCCACTG GCACACGAGT AGTACTGAAG TCTCAGTATG GCTATGAGAT AGATGAGGTG 
AAAGTCATGG GAAAGGATCA ATATCTTGTG GCTCATACCT CTGACACACT GCTACTGGGG 
GATCTGGTGT CCAACAAACT AAGTGAGGTG GCGTGGCAGG GCTCTGGGGG AAATGAAAAG 
TTTTTCTTTG AGAATGAAAC GGTGTGTATG ATTTTCAATG CTGGAGAGCT GGCACTGGTG 
GAATATGGAA GCAATGACAT CCTGGGATCT GTTCGAACAG AGTTTATAAA CCCACACCTT 
ATCAGTGTGC GTCTGAATGA GAGGAGGCAG AGGGGAGTGG AGGATAACAA AAAGTTGGCC 
TACCTGATTG ACATCAAAAC TATTGCTATC GTGGACCTGG TGGGGGGGTA TAATCTAGGC 
ACAATAAGCC ATGACTCCAA GATAGACTGG CTGGAGCTGA ATGAAACGGG CCGTAAACTG 
CTTTTTAGGG ACAAGAAACT CAGGCTCCAC CTTTACGATA TAGAGAGTAG TGTGAAGAGC 
ACCATGCTGA GTTTCTGCCC TTATGTTCAG TGGGTACCGG GCAGTGATGT TGTTGTGGCT 
CAGAATAGAG GAAACCTGTG TGTTTGGTAC ACCATCGACA GCCCAGAGAG GGTCACAATG 
TTTCCCCTCA AGGGTGATAT TGTAGACCTG GAGAGATCTA ATGGTAAAAC CGAGGTCATT 
GTGAATGAAG GGGTTAACTC AATCTCCTAC ACTCTGGATG AGGGACTCAT TGAATTTGGC 
ACAGCAATTG ACGATGGGGA TTATTACAGA GCCACAGCAT TCCTGGAGAC CCTGGAGATG 
TCTGCAGAGA CAGAGGCCAT GTGGAAGACT CTGAGTAAAC TCTCCCTGGA GGCTCAACAG 
CTCCACATCG CTGAGAGGTG TTTTGCAGCC CTTGGCGATG TGTCCAAAGC CCGTTTCCTA 
AATGAGACCA ATAAGATTGC AGATGCGGTT GCAAAAGAAT ATGATGGTGA TGGAACAGAC 
CACTATCAGG TGAAAGCACG GCTGGCCATG CTGGATAAGA ACTTTAAGCT TGCAGAGATG 
TATTACATGG AGCAGAATGC AGTGGATGAA GTCATGGAAA TGTACCAAGA GCTCCACATG 
TGGGATGAAT GTATTGCAGT GGCAGAAGGA AAGGGCCACC CAGAGTTGGA TAATTTGAGA 
CGCAGTTATT ATTCTTACCT AATGGAGACC AATCAGAATG AGAAAGCCGC TGAGGTGAAG 
GAGAACGAAG GAGACTTCAC CGGTGCTGTT AACCTCTACC TTAAAGCAGG ACTACCTGCC 
AAAGCAGCTT GGTTGGCCAT GAGCAAAGAT GAGCTTCTGT CAAGCCAGGA CATTATCAGC 
CGCATCACTG CTGCACTCAT CAAAGGAGAG TTTTATGAGA GGGCTGGAGA CCTGTTTGAA 
AGGACAAGAA ATAATCAGAG AGCCCTGGAG TGTTACCGCA AAGGAAATGC TTTTAAGAAA 
GCGGTGGATT TGGCTCGGGT TGCCTTTCCT GCAGAAGTTG TGAAGCTGGA GGAAGTGTGG 
GGTGACTACT TAGTGCAGCA GAAACAAATG GATGCTGCCA TCAACCATTA CATCGAAGCT 
GGACGCTCCA CCAAGGCGAT AGAAGCAGCT ATTGCAGCTC GTCAGTGGAA GAAAGCATTG 
CACATTCTAG AGCTACAGGA GGACAGGACT GCTGCCAAAT ATTACCTGAA GATTGCCCAG 
CACTACGCCT CTCTGCAGGA ATTTGAGGTA GCAGAGCGTC TGTTTGTCAA GGGTGATCAC 
ATTAAAGATG CTATAGATAT GTACACTCAG GCTGGCCGAT GGGAGCAAGC TCATAAGCTT 
GCTGTAAAGT GTATGACACA AGAAGATGTG TCTGTCCTCT ACGTCAGCAG AGCACAAGAG 
CTAGAGAAAG AGGGCAAGTA CAAAGAAGCC GAAAGGCTTT TTACAACAGT AGATGAACCA 
GATCTCGCCA TCACTATGTA CAAGAAGAAC AAGATGTATG ATGACATGAT TCGTTTAGTT 
GCTGTTCATC ACAAAGACCT GCTGCAGGAA ACACACATTC ACCTGGCCAA GGAACTAGAG 
GCTGAATCCA GGTTTCAGGA AGCCGAGTAT CACTACCTTG AGGGTCAGGA CTGGAAAGCT 
GCTGTCAACA TGTACAGAGT CAATGATATG TGGGAGGAAG CTTATCGGAT AGCAAAAACC 
CACGGAGGAG CCAATGCCCA CAAGCAAGTG GCCTACCTGT GGGCAAAAAG CCTCGGAGGA 
GAGGCAGCAG TTAAACTCCT CAACAAGTTT GGCCTTCTGG AAACTGCCAT TGACTTTGCA 
GCAGATAACT ATACGTTTGA CTTTGCCTTT GAGTTGGCTC GACTCTCCAT GAAGCAGAAG 
ATTCCTGATA TCCATCTCAA AAATGCCATG TTCCTTGAGG ACCAGGGCAA TTTCAATGAG 
GCTGAAATTG AGTTCATCAA AGCTGGAAAA CCCAAAGAGG CTGTGCTAAT GCACGTTCAT 
AACAAGGACT GGAGTAATGC CCAGAGAGTT GCAGAGGCTC ATGATCCGGA AAGTGTGGCA 
GACATTCTTG TGAGCCAGGC CAAGTTCTGC TTTGACCAGA AGGAATTTCA AAAAGCTGAG 
GCCTTCTTGC TCAGGGCACA AAGGCCGGAG CTGGCTATTA AATACTACAA AGATGCTGGG 
ATGTGGACTG ATGCAATGCG GATCTGCAAA GACTATTTGC CCAGCAAGCT GAGCGTCCTT 
CAAAAGGAAT ATGAAAGCGA GGGCAACTGG GGTGTGGAAG GGATGATTGA GCAAGCTCAA 
GAGTGGGAAC AGACCGGAGA CTATTCAAGA GCTGTGGACT GCTATCTGAA AGTGAAGGAC 
TCATCTAACT TGGACTTGCT GTTGAAGTGT TGGATGAAGG CAGCGGAATT GGCCATTAAA 
TTTTTATCCC ATGACAAGGC TGTGGACGTA AGTCAGATTG TCGGTCCACG TCTGATTCAG 
CTCCGAAAGT ACAACGAGGC TGCTGAGCTC TACCTCAACC TAGACCTCAT TAAGAACGCC 
ATAGACGCAT TCATTGAGGG GGAAGAATGG AACAAAGCCA AAAGAGTGGC CAAGGAGCTG 
GATCCAAGAC TTGAGGAGTA TGTGGACAAA CGCTACAAAG AGCACCTGAA AAATCAAGGC 
AAAGTTGACT CTTTGGTTGG AGTAGATGTG GTTGCTGCAC TGGACATGTA TGCAGAAAGA 
GGACAGTGGG AGAAATGTAT TGACACAGCC TCCAAACAGA ATTTTAAGGT GCTGCACAAG 
TACATCGCCC TGTACGCCAC CCACCTGATC AAAGAAGGTG AGGTGGAGAA AGTGCTCAGC 
CTGTACATCC AGCATGGAGT TCCAGCTTAT TCACAGAACT TCAACATCTA CAAGCGGATG 
TTCCAGGAGC TGGTGAACCT AAGGGACCGG GATTGTGCGG AAGCCTTTCG GATGTGGTCA 
GATCTACGGG ACGTCCTTCT GTTGTTGTGT GAAAACCTGA CCAAGTCATC AGAAGCTAAT 
TCTCCAGCGC ATGAGGAGTT TGAGCAGATG TTGCTGGTTG CTCACTATTA CGCCACTCGT 
TCTGCAGCCA AAGGCATTGA CCAGCTGAGT TCTGTGGCGG CCAAGCTGTC CATCTCTCTC 
CTGAGGCACA CCGAGCTCAT TCCAGCTGAC AAGGCCTTTT ATGAGGCTGG CCTGGCAGCC 
AAGGCTGTTG GATGGGAGAA CATGGCTTTT ATCTTCCTCA ATCGCTTCTT GGATTTGGCT 
GATGGCATTG ATGAGGGAAC ACTGGATGCT TTAGATCACA CAGACTTCCA GGATACAGAC 
ATCCCATTTG AGGTCCCTGT TCCTTCCAGG CTACACGTCA CAGTGGAGAA GAGGGAGGAG 
ATTAGAGAAT GGGTTCTGAC TGTGTCCATG GACCAGCGGG TGGAGCAGGT GCTGCCGAAG 
GATGAGAGGG GCACGTACGA AGCCTCCCTC GTGGCCGCCA GCACTGGCAT CCGGTCCCTA 
CCTTGCGTCA TCACAGGTTA CCCCGTCCTC CGTAATAAAA TTGAGTTCAA AAGACCAGGC 
ATGGCAGCCA ATAAAGATGA CTGGAATAAA TTCTTGATGG CTACAAAGAC CACTCACAGT 
CCAGAATGTC AAGATGTGCT GAAATTTATC ACGCAGTGGT GTGGAGGTCT CCCATCAGCT 
GGATATTCCT TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002312.1
CDS	121..5358
Translation	MQLKYMKTLLTPQDGAAKVTCMAWAPNNAKFAVCTIDRVVILYDEQGEKRDRFTTKPADPKYGKKTYVVKSMAFSPDSTKIAVAQTDNIIFVYKIGEEWGDKKVICNKFIQTSAVTSLVWPSEHAIIFGLAEGKVRQANTKTSKSSTIYGTDSYVVSLTSNVSGKGILSGHADGTVVRYFIDDEGSGESQGKLLTHACPPYALAWGTNSIIVAGCDKKIVAYGKEGNVIQTFDYSRDRAEKEFTVAASSPSGQSIVVGSFDRLRVFNWSPRKGTWDESSPKEIPNLYTITALSWKKDGSRLSVGTLCGGVEMFDCCLRRSIYKNKFEMTYVGLSQVIVRNLSTGTRVVLKSQYGYEIDEVKVMGKDQYLVAHTSDTLLLGDLVSNKLSEVAWQGSGGNEKFFFENETVCMIFNAGELALVEYGSNDILGSVRTEFINPHLISVRLNERRQRGVEDNKKLAYLIDIKTIAIVDLVGGYNLGTISHDSKIDWLELNETGRKLLFRDKKLRLHLYDIESSVKSTMLSFCPYVQWVPGSDVVVAQNRGNLCVWYTIDSPERVTMFPLKGDIVDLERSNGKTEVIVNEGVNSISYTLDEGLIEFGTAIDDGDYYRATAFLETLEMSAETEAMWKTLSKLSLEAQQLHIAERCFAALGDVSKARFLNETNKIADAVAKEYDGDGTDHYQVKARLAMLDKNFKLAEMYYMEQNAVDEVMEMYQELHMWDECIAVAEGKGHPELDNLRRSYYSYLMETNQNEKAAEVKENEGDFTGAVNLYLKAGLPAKAAWLAMSKDELLSSQDIISRITAALIKGEFYERAGDLFERTRNNQRALECYRKGNAFKKAVDLARVAFPAEVVKLEEVWGDYLVQQKQMDAAINHYIEAGRSTKAIEAAIAARQWKKALHILELQEDRTAAKYYLKIAQHYASLQEFEVAERLFVKGDHIKDAIDMYTQAGRWEQAHKLAVKCMTQEDVSVLYVSRAQELEKEGKYKEAERLFTTVDEPDLAITMYKKNKMYDDMIRLVAVHHKDLLQETHIHLAKELEAESRFQEAEYHYLEGQDWKAAVNMYRVNDMWEEAYRIAKTHGGANAHKQVAYLWAKSLGGEAAVKLLNKFGLLETAIDFAADNYTFDFAFELARLSMKQKIPDIHLKNAMFLEDQGNFNEAEIEFIKAGKPKEAVLMHVHNKDWSNAQRVAEAHDPESVADILVSQAKFCFDQKEFQKAEAFLLRAQRPELAIKYYKDAGMWTDAMRICKDYLPSKLSVLQKEYESEGNWGVEGMIEQAQEWEQTGDYSRAVDCYLKVKDSSNLDLLLKCWMKAAELAIKFLSHDKAVDVSQIVGPRLIQLRKYNEAAELYLNLDLIKNAIDAFIEGEEWNKAKRVAKELDPRLEEYVDKRYKEHLKNQGKVDSLVGVDVVAALDMYAERGQWEKCIDTASKQNFKVLHKYIALYATHLIKEGEVEKVLSLYIQHGVPAYSQNFNIYKRMFQELVNLRDRDCAEAFRMWSDLRDVLLLLCENLTKSSEANSPAHEEFEQMLLVAHYYATRSAAKGIDQLSSVAAKLSISLLRHTELIPADKAFYEAGLAAKAVGWENMAFIFLNRFLDLADGIDEGTLDALDHTDFQDTDIPFEVPVPSRLHVTVEKREEIREWVLTVSMDQRVEQVLPKDERGTYEASLVAASTGIRSLPCVITGYPVLRNKIEFKRPGMAANKDDWNKFLMATKTTHSPECQDVLKFITQWCGGLPSAGYSFH

Target ORF information:

RefSeq Version	NM_001002312.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intraflagellar transport 172 (ift172), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002312.1

ORF Insert Sequence:

ATGCAGCTTA AGTACATGAA GACTCTTTTA ACGCCTCAGG ATGGAGCTGC TAAAGTCACC 
TGTATGGCTT GGGCTCCAAA CAATGCAAAG TTTGCAGTGT GCACTATTGA TAGAGTGGTT 
ATTTTGTATG ATGAGCAGGG AGAGAAAAGA GACAGATTTA CAACAAAGCC TGCTGACCCA 
AAATACGGAA AGAAAACCTA TGTGGTCAAG TCTATGGCAT TTTCCCCTGA TTCAACCAAG 
ATAGCAGTTG CTCAAACAGA CAACATCATA TTTGTTTACA AAATTGGAGA GGAGTGGGGA 
GATAAAAAGG TTATTTGCAA CAAGTTCATT CAAACAAGTG CTGTTACAAG CCTGGTTTGG 
CCGTCTGAGC ATGCAATTAT ATTTGGACTA GCAGAAGGAA AAGTTCGACA AGCTAATACA 
AAAACAAGCA AGTCCTCAAC AATCTATGGA ACAGACTCTT ATGTTGTCTC ACTAACCTCA 
AATGTATCAG GAAAGGGAAT CCTTTCCGGT CACGCAGATG GAACGGTGGT TCGGTATTTC 
ATTGATGATG AAGGATCTGG GGAATCTCAG GGCAAACTGC TGACTCATGC ATGTCCACCA 
TATGCCTTAG CATGGGGCAC AAACAGCATA ATTGTGGCTG GGTGCGATAA GAAGATTGTT 
GCCTATGGGA AGGAGGGCAA CGTGATTCAG ACATTTGATT ACAGCCGAGA TCGAGCCGAA 
AAGGAATTCA CTGTGGCTGC ATCGAGCCCT AGTGGCCAGT CCATAGTTGT AGGAAGCTTT 
GACAGATTGC GAGTTTTTAA TTGGAGTCCT CGAAAAGGCA CATGGGATGA ATCTTCTCCC 
AAAGAAATTC CAAACCTGTA CACCATCACA GCTCTCTCCT GGAAAAAAGA TGGCTCTCGG 
CTTTCTGTGG GCACTCTGTG TGGAGGTGTC GAGATGTTTG ACTGCTGTCT CCGACGGAGT 
ATTTACAAAA ACAAATTTGA GATGACATAC GTAGGACTAA GTCAGGTGAT TGTGAGGAAT 
TTGTCCACTG GCACACGAGT AGTACTGAAG TCTCAGTATG GCTATGAGAT AGATGAGGTG 
AAAGTCATGG GAAAGGATCA ATATCTTGTG GCTCATACCT CTGACACACT GCTACTGGGG 
GATCTGGTGT CCAACAAACT AAGTGAGGTG GCGTGGCAGG GCTCTGGGGG AAATGAAAAG 
TTTTTCTTTG AGAATGAAAC GGTGTGTATG ATTTTCAATG CTGGAGAGCT GGCACTGGTG 
GAATATGGAA GCAATGACAT CCTGGGATCT GTTCGAACAG AGTTTATAAA CCCACACCTT 
ATCAGTGTGC GTCTGAATGA GAGGAGGCAG AGGGGAGTGG AGGATAACAA AAAGTTGGCC 
TACCTGATTG ACATCAAAAC TATTGCTATC GTGGACCTGG TGGGGGGGTA TAATCTAGGC 
ACAATAAGCC ATGACTCCAA GATAGACTGG CTGGAGCTGA ATGAAACGGG CCGTAAACTG 
CTTTTTAGGG ACAAGAAACT CAGGCTCCAC CTTTACGATA TAGAGAGTAG TGTGAAGAGC 
ACCATGCTGA GTTTCTGCCC TTATGTTCAG TGGGTACCGG GCAGTGATGT TGTTGTGGCT 
CAGAATAGAG GAAACCTGTG TGTTTGGTAC ACCATCGACA GCCCAGAGAG GGTCACAATG 
TTTCCCCTCA AGGGTGATAT TGTAGACCTG GAGAGATCTA ATGGTAAAAC CGAGGTCATT 
GTGAATGAAG GGGTTAACTC AATCTCCTAC ACTCTGGATG AGGGACTCAT TGAATTTGGC 
ACAGCAATTG ACGATGGGGA TTATTACAGA GCCACAGCAT TCCTGGAGAC CCTGGAGATG 
TCTGCAGAGA CAGAGGCCAT GTGGAAGACT CTGAGTAAAC TCTCCCTGGA GGCTCAACAG 
CTCCACATCG CTGAGAGGTG TTTTGCAGCC CTTGGCGATG TGTCCAAAGC CCGTTTCCTA 
AATGAGACCA ATAAGATTGC AGATGCGGTT GCAAAAGAAT ATGATGGTGA TGGAACAGAC 
CACTATCAGG TGAAAGCACG GCTGGCCATG CTGGATAAGA ACTTTAAGCT TGCAGAGATG 
TATTACATGG AGCAGAATGC AGTGGATGAA GTCATGGAAA TGTACCAAGA GCTCCACATG 
TGGGATGAAT GTATTGCAGT GGCAGAAGGA AAGGGCCACC CAGAGTTGGA TAATTTGAGA 
CGCAGTTATT ATTCTTACCT AATGGAGACC AATCAGAATG AGAAAGCCGC TGAGGTGAAG 
GAGAACGAAG GAGACTTCAC CGGTGCTGTT AACCTCTACC TTAAAGCAGG ACTACCTGCC 
AAAGCAGCTT GGTTGGCCAT GAGCAAAGAT GAGCTTCTGT CAAGCCAGGA CATTATCAGC 
CGCATCACTG CTGCACTCAT CAAAGGAGAG TTTTATGAGA GGGCTGGAGA CCTGTTTGAA 
AGGACAAGAA ATAATCAGAG AGCCCTGGAG TGTTACCGCA AAGGAAATGC TTTTAAGAAA 
GCGGTGGATT TGGCTCGGGT TGCCTTTCCT GCAGAAGTTG TGAAGCTGGA GGAAGTGTGG 
GGTGACTACT TAGTGCAGCA GAAACAAATG GATGCTGCCA TCAACCATTA CATCGAAGCT 
GGACGCTCCA CCAAGGCGAT AGAAGCAGCT ATTGCAGCTC GTCAGTGGAA GAAAGCATTG 
CACATTCTAG AGCTACAGGA GGACAGGACT GCTGCCAAAT ATTACCTGAA GATTGCCCAG 
CACTACGCCT CTCTGCAGGA ATTTGAGGTA GCAGAGCGTC TGTTTGTCAA GGGTGATCAC 
ATTAAAGATG CTATAGATAT GTACACTCAG GCTGGCCGAT GGGAGCAAGC TCATAAGCTT 
GCTGTAAAGT GTATGACACA AGAAGATGTG TCTGTCCTCT ACGTCAGCAG AGCACAAGAG 
CTAGAGAAAG AGGGCAAGTA CAAAGAAGCC GAAAGGCTTT TTACAACAGT AGATGAACCA 
GATCTCGCCA TCACTATGTA CAAGAAGAAC AAGATGTATG ATGACATGAT TCGTTTAGTT 
GCTGTTCATC ACAAAGACCT GCTGCAGGAA ACACACATTC ACCTGGCCAA GGAACTAGAG 
GCTGAATCCA GGTTTCAGGA AGCCGAGTAT CACTACCTTG AGGGTCAGGA CTGGAAAGCT 
GCTGTCAACA TGTACAGAGT CAATGATATG TGGGAGGAAG CTTATCGGAT AGCAAAAACC 
CACGGAGGAG CCAATGCCCA CAAGCAAGTG GCCTACCTGT GGGCAAAAAG CCTCGGAGGA 
GAGGCAGCAG TTAAACTCCT CAACAAGTTT GGCCTTCTGG AAACTGCCAT TGACTTTGCA 
GCAGATAACT ATACGTTTGA CTTTGCCTTT GAGTTGGCTC GACTCTCCAT GAAGCAGAAG 
ATTCCTGATA TCCATCTCAA AAATGCCATG TTCCTTGAGG ACCAGGGCAA TTTCAATGAG 
GCTGAAATTG AGTTCATCAA AGCTGGAAAA CCCAAAGAGG CTGTGCTAAT GCACGTTCAT 
AACAAGGACT GGAGTAATGC CCAGAGAGTT GCAGAGGCTC ATGATCCGGA AAGTGTGGCA 
GACATTCTTG TGAGCCAGGC CAAGTTCTGC TTTGACCAGA AGGAATTTCA AAAAGCTGAG 
GCCTTCTTGC TCAGGGCACA AAGGCCGGAG CTGGCTATTA AATACTACAA AGATGCTGGG 
ATGTGGACTG ATGCAATGCG GATCTGCAAA GACTATTTGC CCAGCAAGCT GAGCGTCCTT 
CAAAAGGAAT ATGAAAGCGA GGGCAACTGG GGTGTGGAAG GGATGATTGA GCAAGCTCAA 
GAGTGGGAAC AGACCGGAGA CTATTCAAGA GCTGTGGACT GCTATCTGAA AGTGAAGGAC 
TCATCTAACT TGGACTTGCT GTTGAAGTGT TGGATGAAGG CAGCGGAATT GGCCATTAAA 
TTTTTATCCC ATGACAAGGC TGTGGACGTA AGTCAGATTG TCGGTCCACG TCTGATTCAG 
CTCCGAAAGT ACAACGAGGC TGCTGAGCTC TACCTCAACC TAGACCTCAT TAAGAACGCC 
ATAGACGCAT TCATTGAGGG GGAAGAATGG AACAAAGCCA AAAGAGTGGC CAAGGAGCTG 
GATCCAAGAC TTGAGGAGTA TGTGGACAAA CGCTACAAAG AGCACCTGAA AAATCAAGGC 
AAAGTTGACT CTTTGGTTGG AGTAGATGTG GTTGCTGCAC TGGACATGTA TGCAGAAAGA 
GGACAGTGGG AGAAATGTAT TGACACAGCC TCCAAACAGA ATTTTAAGGT GCTGCACAAG 
TACATCGCCC TGTACGCCAC CCACCTGATC AAAGAAGGTG AGGTGGAGAA AGTGCTCAGC 
CTGTACATCC AGCATGGAGT TCCAGCTTAT TCACAGAACT TCAACATCTA CAAGCGGATG 
TTCCAGGAGC TGGTGAACCT AAGGGACCGG GATTGTGCGG AAGCCTTTCG GATGTGGTCA 
GATCTACGGG ACGTCCTTCT GTTGTTGTGT GAAAACCTGA CCAAGTCATC AGAAGCTAAT 
TCTCCAGCGC ATGAGGAGTT TGAGCAGATG TTGCTGGTTG CTCACTATTA CGCCACTCGT 
TCTGCAGCCA AAGGCATTGA CCAGCTGAGT TCTGTGGCGG CCAAGCTGTC CATCTCTCTC 
CTGAGGCACA CCGAGCTCAT TCCAGCTGAC AAGGCCTTTT ATGAGGCTGG CCTGGCAGCC 
AAGGCTGTTG GATGGGAGAA CATGGCTTTT ATCTTCCTCA ATCGCTTCTT GGATTTGGCT 
GATGGCATTG ATGAGGGAAC ACTGGATGCT TTAGATCACA CAGACTTCCA GGATACAGAC 
ATCCCATTTG AGGTCCCTGT TCCTTCCAGG CTACACGTCA CAGTGGAGAA GAGGGAGGAG 
ATTAGAGAAT GGGTTCTGAC TGTGTCCATG GACCAGCGGG TGGAGCAGGT GCTGCCGAAG 
GATGAGAGGG GCACGTACGA AGCCTCCCTC GTGGCCGCCA GCACTGGCAT CCGGTCCCTA 
CCTTGCGTCA TCACAGGTTA CCCCGTCCTC CGTAATAAAA TTGAGTTCAA AAGACCAGGC 
ATGGCAGCCA ATAAAGATGA CTGGAATAAA TTCTTGATGG CTACAAAGAC CACTCACAGT 
CCAGAATGTC AAGATGTGCT GAAATTTATC ACGCAGTGGT GTGGAGGTCT CCCATCAGCT 
GGATATTCCT TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one9(12)e114632(2014)
Thomas HR,Percival SM,Yoder BK,Parant JM

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ift172 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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