MDH1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDH1
Entrez Gene ID	694703
Full Name	malate dehydrogenase 1
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)
Summary	This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320105.1	NP_001307034.1	malate dehydrogenase, peroxisomal isoform MDH1x
NM_001320104.1	NP_001307033.1	malate dehydrogenase, cytoplasmic isoform MDH1
NM_001320106.1	NP_001307035.1	malate dehydrogenase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MDH1	XP_531844.1
Bos taurus (cattle)	MDH1	NP_001029800.1
Caenorhabditis elegans (roundworm)	mdh-1	NP_504656.1
Xenopus tropicalis (tropical clawed frog)	mdh1	NP_001006694.1
	MDH1	XP_001084973.1
Arabidopsis thaliana (thale cress)	AT5G43330	NP_199147.1
Pan troglodytes (chimpanzee)	MDH1	NP_001229997.1
Homo sapiens (human)	MDH1	NP_001186040.1
Gallus gallus (chicken)	MDH1	NP_001006395.1
Oryza sativa (rice)	Os10g0478200	NP_001064860.1
Mus musculus (house mouse)	Mdh1	NP_032644.3
Rattus norvegicus (Norway rat)	Mdh1	NP_150238.1
Danio rerio (zebrafish)	mdh1b	NP_956263.1
Drosophila melanogaster (fruit fly)	Mdh1	NP_609394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009510	XP_310186.3
Arabidopsis thaliana (thale cress)	AT1G04410	NP_171936.1

Gene Ontology
Bibliography

Related articles in PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Zimin AV, Cornish AS, Maudhoo MD, Gibbs RM, Zhang X, Pandey S, Meehan DT, Wipfler K, Bosinger SE, Johnson ZP, Tharp GK, Mar?ais G, Roberts M, Ferguson B, Fox HS, Treangen T, Salzberg SL, Yorke JA, Norgren RB Jr
Biology direct9(1)20(2014 Oct)

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Magness CL, Fellin PC, Thomas MJ, Korth MJ, Agy MB, Proll SC, Fitzgibbon M, Scherer CA, Miner DG, Katze MG, Iadonato SP
Genome biology6(7)R60(2005)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MDH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb57527	NM_001320104.1 Latest version!	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMb57528	NM_001320106.1 Latest version!	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMb57566	NM_001320105.1 Latest version!	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDH1 ( NM_001320104.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001307033.1 Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb57527
Clone ID Related Accession (Same CDS sequence)	NM_001320104.1
Accession Version	NM_001320104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	malate dehydrogenase, cytoplasmic isoform MDH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD766800.1, JV490880.1 and CN647359.1. On Feb 11, 2016 this sequence version replaced XM_015112577.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MDH1), which is localized in the cytosol. ##Evidence-Data-START## Transcript exon combination :: JU475632.1, JV044980.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTATAGTA TTGGAAATGG ATCCGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTCGT GGGCTCCATG CCAAGAAGAG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA CAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AACCCAGCCA ATACCAACTG CCTGACTGCG 
TCCAAGTCGG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAC GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCAA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAATTGGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTC 
ACAGAAGAAA AAGAAACTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307033.1
CDS	82..1086
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKLVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA

Target ORF information:

RefSeq Version	NM_001320104.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320104.1

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTATAGTA TTGGAAATGG ATCCGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTCGT GGGCTCCATG CCAAGAAGAG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA CAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AACCCAGCCA ATACCAACTG CCTGACTGCG 
TCCAAGTCGG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAC GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCAA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAATTGGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTC 
ACAGAAGAAA AAGAAACTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_001320106.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001307035.1 Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMb57528
Clone ID Related Accession (Same CDS sequence)	NM_001320106.1
Accession Version	NM_001320106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	malate dehydrogenase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD766800.1, CB311144.1, JV490880.1 and CN647359.1. On Feb 11, 2016 this sequence version replaced XM_015112576.1. Transcript Variant: This variant (2) contains an additional exon in the 5' region, which results in translation initiation from an alternate start codon compared to variant 1. The encoded isoform (2) is longer with a distinct N-terminus compared to isoform MDH1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTTT GTTTAATGAA TGAATTAAAT GAGACAACAG CTCATCAGTG GATTTATAAG 
TCTGAACCAA TCAGAGTCCT TGTGACTGGA GCAGCTGGTC AAATTGCATA TTCACTGCTG 
TATAGTATTG GAAATGGATC CGTCTTTGGT AAAGATCAGC CTATAATTCT TGTGCTGTTG 
GATATCACCC CCATGATGGG TGTCCTGGAC GGTGTCCTAA TGGAACTGCA AGACTGTGCC 
CTTCCCCTCC TGAAAGATGT CATCGCAACA GATAAAGAAG AGGTTGCCTT CAAAGACCTG 
GATGTGGCCA TTCTCGTGGG CTCCATGCCA AGAAGAGAAG GCATGGAGAG AAAAGATTTA 
CTGAAAGCAA ATGTGAAAAT CTTCAAATCC CAGGGTGCAG CCTTAGACAA ATACGCCAAG 
AAGTCAGTTA AGGTTATTGT TGTGGGTAAC CCAGCCAATA CCAACTGCCT GACTGCGTCC 
AAGTCGGCTC CATCCATCCC CAAGGAGAAC TTCAGTTGCT TGACTCGTTT GGATCACAAC 
CGAGCTAAAG CTCAAATTGC TCTTAAACTT GGTGTGACTG CTAATGATGT AAAGAATGTC 
ATTATCTGGG GAAACCATTC CTCGACTCAG TATCCAGATG TCAACCATGC CAAGGTGAAA 
TTGCAAGGAA AGGAAGTTGG TGTTTACGAA GCTCTGAAAG ATGACAGCTG GCTCAAGGGA 
GAATTTGTCA CGACTGTGCA GCAGCGTGGC GCTGCTGTCA TCAAGGCTCG AAAACTATCC 
AGTGCAATGT CTGCTGCAAA AGCCATCTGT GACCACGTCA GGGACATCTG GTTTGGAACC 
CCAGAGGGAG AGTTTGTGTC CATGGGTGTT ATCTCTGATG GCAACTCCTA TGGTGTTCCT 
GATGATCTGC TCTACTCATT CCCTGTTGTA ATCAAGAATA AGACCTGGAA ATTGGTTGAA 
GGTCTCCCTA TTAATGATTT CTCACGTGAG AAGATGGATC TTACTGCAAA GGAACTCACA 
GAAGAAAAAG AAACTGCTTT TGAATTTCTT TCCTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307035.1
CDS	186..1247
Translation	MLFCLMNELNETTAHQWIYKSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKLVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA

Target ORF information:

RefSeq Version	NM_001320106.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320106.1

ORF Insert Sequence:

ATGCTGTTTT GTTTAATGAA TGAATTAAAT GAGACAACAG CTCATCAGTG GATTTATAAG 
TCTGAACCAA TCAGAGTCCT TGTGACTGGA GCAGCTGGTC AAATTGCATA TTCACTGCTG 
TATAGTATTG GAAATGGATC CGTCTTTGGT AAAGATCAGC CTATAATTCT TGTGCTGTTG 
GATATCACCC CCATGATGGG TGTCCTGGAC GGTGTCCTAA TGGAACTGCA AGACTGTGCC 
CTTCCCCTCC TGAAAGATGT CATCGCAACA GATAAAGAAG AGGTTGCCTT CAAAGACCTG 
GATGTGGCCA TTCTCGTGGG CTCCATGCCA AGAAGAGAAG GCATGGAGAG AAAAGATTTA 
CTGAAAGCAA ATGTGAAAAT CTTCAAATCC CAGGGTGCAG CCTTAGACAA ATACGCCAAG 
AAGTCAGTTA AGGTTATTGT TGTGGGTAAC CCAGCCAATA CCAACTGCCT GACTGCGTCC 
AAGTCGGCTC CATCCATCCC CAAGGAGAAC TTCAGTTGCT TGACTCGTTT GGATCACAAC 
CGAGCTAAAG CTCAAATTGC TCTTAAACTT GGTGTGACTG CTAATGATGT AAAGAATGTC 
ATTATCTGGG GAAACCATTC CTCGACTCAG TATCCAGATG TCAACCATGC CAAGGTGAAA 
TTGCAAGGAA AGGAAGTTGG TGTTTACGAA GCTCTGAAAG ATGACAGCTG GCTCAAGGGA 
GAATTTGTCA CGACTGTGCA GCAGCGTGGC GCTGCTGTCA TCAAGGCTCG AAAACTATCC 
AGTGCAATGT CTGCTGCAAA AGCCATCTGT GACCACGTCA GGGACATCTG GTTTGGAACC 
CCAGAGGGAG AGTTTGTGTC CATGGGTGTT ATCTCTGATG GCAACTCCTA TGGTGTTCCT 
GATGATCTGC TCTACTCATT CCCTGTTGTA ATCAAGAATA AGACCTGGAA ATTGGTTGAA 
GGTCTCCCTA TTAATGATTT CTCACGTGAG AAGATGGATC TTACTGCAAA GGAACTCACA 
GAAGAAAAAG AAACTGCTTT TGAATTTCTT TCCTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_001320105.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001307034.1 Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb57566
Clone ID Related Accession (Same CDS sequence)	NM_001320105.1
Accession Version	NM_001320105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	malate dehydrogenase, peroxisomal isoform MDH1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD766800.1, JV490880.1 and CN647359.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (MDH1x), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: JU475632.1, JV044980.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTATAGTA TTGGAAATGG ATCCGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTCGT GGGCTCCATG CCAAGAAGAG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA CAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AACCCAGCCA ATACCAACTG CCTGACTGCG 
TCCAAGTCGG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAC GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCAA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAATTGGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTC 
ACAGAAGAAA AAGAAACTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAAGGATGTT 
ACTAAGTGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307034.1
CDS	82..1143
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKLVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA*LDKDVTKCFKAEESKCRL

Target ORF information:

RefSeq Version	NM_001320105.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320105.1

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTATAGTA TTGGAAATGG ATCCGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTCGT GGGCTCCATG CCAAGAAGAG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA CAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AACCCAGCCA ATACCAACTG CCTGACTGCG 
TCCAAGTCGG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAC GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCAA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAATTGGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTC 
ACAGAAGAAA AAGAAACTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAAGGATGTT 
ACTAAGTGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Biology direct9(1)20(2014 Oct)
Zimin AV,Cornish AS,Maudhoo MD,Gibbs RM,Zhang X,Pandey S,Meehan DT,Wipfler K,Bosinger SE,Johnson ZP,Tharp GK,Mar?ais G,Roberts M,Ferguson B,Fox HS,Treangen T,Salzberg SL,Yorke JA,Norgren RB Jr

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
PLoS genetics10(10)e1004685(2014 Oct)
Stiebler AC,Freitag J,Schink KO,Stehlik T,Tillmann BA,Ast J,B?lker M

Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Genome biology6(7)R60(2005)
Magness CL,Fellin PC,Thomas MJ,Korth MJ,Agy MB,Proll SC,Fitzgibbon M,Scherer CA,Miner DG,Katze MG,Iadonato SP

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

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MDH1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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