Mdh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdh1
Entrez Gene ID	34414
Full Name	Malate dehydrogenase 1
Synonyms	CG5362,CT17038,Dmel\CG5362,MDH,MDH-1,Mdh,Mdh-1,Mdh2,MdhD,cMdh,sMdh
General protein information	Preferred Names Malate dehydrogenase 1 Names CG5362 gene product from transcript CG5362-RB CG5362-PA CG5362-PB Mdh1-PA Mdh1-PB cytoplasmic Mdh cytosolic malate dehydrogenase malate dehydrogenase malate dehydrogenase 1 malic DH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31E1-31E1\|2-37.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298874.1	NP_001285803.1	malate dehydrogenase 1, isoform B
NM_135550.2	NP_609394.1	malate dehydrogenase 1, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MDH1	XP_531844.1
Bos taurus (cattle)	MDH1	NP_001029800.1
Caenorhabditis elegans (roundworm)	mdh-1	NP_504656.1
Xenopus tropicalis (tropical clawed frog)	mdh1	NP_001006694.1
	MDH1	XP_001084973.1
Arabidopsis thaliana (thale cress)	AT5G43330	NP_199147.1
Pan troglodytes (chimpanzee)	MDH1	NP_001229997.1
Homo sapiens (human)	MDH1	NP_001186040.1
Gallus gallus (chicken)	MDH1	NP_001006395.1
Oryza sativa (rice)	Os10g0478200	NP_001064860.1
Mus musculus (house mouse)	Mdh1	NP_032644.3
Rattus norvegicus (Norway rat)	Mdh1	NP_150238.1
Danio rerio (zebrafish)	mdh1b	NP_956263.1
Drosophila melanogaster (fruit fly)	Mdh1	NP_609394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009510	XP_310186.3
Arabidopsis thaliana (thale cress)	AT1G04410	NP_171936.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mdh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14343	NM_135550.2 Latest version!	Drosophila melanogaster malate dehydrogenase 1, transcript variant A (Mdh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14343	NM_001298874.1 Latest version!	Drosophila melanogaster malate dehydrogenase 1, transcript variant B (Mdh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdh1 ( NM_001298874.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285803.1 Drosophila melanogaster malate dehydrogenase 1, transcript variant B (Mdh1), mRNA.

Price & Availability↑

CloneID	OFa14343
Clone ID Related Accession (Same CDS sequence)	NM_001298874.1 , NM_135550.2
Accession Version	NM_001298874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	malate dehydrogenase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAC CAATTCGTGT TGTGGTGACC GGAGCCGCTG GCCAAATCGC CTACTCCCTG 
CTGTACATGA TTGCCCGCGG CGAGGTGTTC GGCAAGGATC AGCCCATCGT GCTGCACCTG 
CTCGACATTC CGCCCATGGT TGGTGTGCTC GAGGGCGTGG TCATGGAATT GGCCGACTGC 
GCTCTGCCGC TGCTGGTCGA GGTGGTGCCC ACCACCGACC CGGCTGTTGG ATTCAAGGAT 
GTCTCGGCCG CTTTCCTCGT GGGCGCCATG CCCCGCAAGG AGGGAATGGA GCGAAAGGAT 
CTGCTGTCCG CCAACGTGAA GATCTTCAGG ACCCAGGGCC AGGCTCTGGA CAAGTTCGCC 
AAGAAGGACG TCAAGGTGCT GGTTGTGGGC AACCCGGCCA ATACCAATGC CCTGGTCTGC 
TCCTCCTATG CGCCTTCCAT TCCGCGCGAA AATTTCTCGG CTATGACTCG CCTGGATCAG 
AATCGCGCCA CATCCCAGAT CGCCGCCAAA TTGGGTGTGC CAATTTCTGC TGTTAAGAAC 
ATCATTATTT GGGGCAACCA CTCCTCCACC CAGTATCCCG ATGCTGGACA GGCCAAGGTG 
ACCGCCAATG GTACCGTTAA GTCCGTGGTG GATGCGATCA ACGATAATGG CTACCTGCAG 
GGATCCTTCG TGGAAACCGT TCAGAAGCGT GGAGCCGCTG TCATTGCGGC CCGCAAAATG 
TCATCGGCCA TGTCGGCGGC GAAGGCCGCT TGCGATCACA TGCACGACTG GTGGAACGGC 
ACTGCTCCCG GCCAGTTCGT CTCCATGGGT GTCTTCTCCG ACGGCAGCTA CGATTCGCCC 
AAGGATGTGA TCTTCTCGTT CCCCGTCGAG ATCAAGAATA AGCAATGGAA AATCGTCTCT 
GGCCTGACCC TCAGCGATTT CGCTAAGACA AAGTTAAGCG TTACCGGCAA GGAGCTGCAG 
GAGGAGAAGG ACGAGGCTCT GTCCGTGCTG GACTCGAATG TGTCCAACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285803.1
CDS	116..1129
Translation	MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAMPRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPANTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKVTANGTVKSVVDAINDNGYLQGSFVETVQKRGAAVIAARKMSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNKQWKIVSGLTLSDFAKTKLSVTGKELQEEKDEALSVLDSNVSNL

Target ORF information:

RefSeq Version	NM_001298874.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malate dehydrogenase 1, transcript variant B (Mdh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298874.1

ORF Insert Sequence:

ATGGCTGAAC CAATTCGTGT TGTGGTGACC GGAGCCGCTG GCCAAATCGC CTACTCCCTG 
CTGTACATGA TTGCCCGCGG CGAGGTGTTC GGCAAGGATC AGCCCATCGT GCTGCACCTG 
CTCGACATTC CGCCCATGGT TGGTGTGCTC GAGGGCGTGG TCATGGAATT GGCCGACTGC 
GCTCTGCCGC TGCTGGTCGA GGTGGTGCCC ACCACCGACC CGGCTGTTGG ATTCAAGGAT 
GTCTCGGCCG CTTTCCTCGT GGGCGCCATG CCCCGCAAGG AGGGAATGGA GCGAAAGGAT 
CTGCTGTCCG CCAACGTGAA GATCTTCAGG ACCCAGGGCC AGGCTCTGGA CAAGTTCGCC 
AAGAAGGACG TCAAGGTGCT GGTTGTGGGC AACCCGGCCA ATACCAATGC CCTGGTCTGC 
TCCTCCTATG CGCCTTCCAT TCCGCGCGAA AATTTCTCGG CTATGACTCG CCTGGATCAG 
AATCGCGCCA CATCCCAGAT CGCCGCCAAA TTGGGTGTGC CAATTTCTGC TGTTAAGAAC 
ATCATTATTT GGGGCAACCA CTCCTCCACC CAGTATCCCG ATGCTGGACA GGCCAAGGTG 
ACCGCCAATG GTACCGTTAA GTCCGTGGTG GATGCGATCA ACGATAATGG CTACCTGCAG 
GGATCCTTCG TGGAAACCGT TCAGAAGCGT GGAGCCGCTG TCATTGCGGC CCGCAAAATG 
TCATCGGCCA TGTCGGCGGC GAAGGCCGCT TGCGATCACA TGCACGACTG GTGGAACGGC 
ACTGCTCCCG GCCAGTTCGT CTCCATGGGT GTCTTCTCCG ACGGCAGCTA CGATTCGCCC 
AAGGATGTGA TCTTCTCGTT CCCCGTCGAG ATCAAGAATA AGCAATGGAA AATCGTCTCT 
GGCCTGACCC TCAGCGATTT CGCTAAGACA AAGTTAAGCG TTACCGGCAA GGAGCTGCAG 
GAGGAGAAGG ACGAGGCTCT GTCCGTGCTG GACTCGAATG TGTCCAACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdh1 ( NM_135550.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609394.1 Drosophila melanogaster malate dehydrogenase 1, transcript variant A (Mdh1), mRNA.

Price & Availability↑

CloneID	OFa14343
Clone ID Related Accession (Same CDS sequence)	NM_001298874.1 , NM_135550.2
Accession Version	NM_135550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	malate dehydrogenase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135550.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAC CAATTCGTGT TGTGGTGACC GGAGCCGCTG GCCAAATCGC CTACTCCCTG 
CTGTACATGA TTGCCCGCGG CGAGGTGTTC GGCAAGGATC AGCCCATCGT GCTGCACCTG 
CTCGACATTC CGCCCATGGT TGGTGTGCTC GAGGGCGTGG TCATGGAATT GGCCGACTGC 
GCTCTGCCGC TGCTGGTCGA GGTGGTGCCC ACCACCGACC CGGCTGTTGG ATTCAAGGAT 
GTCTCGGCCG CTTTCCTCGT GGGCGCCATG CCCCGCAAGG AGGGAATGGA GCGAAAGGAT 
CTGCTGTCCG CCAACGTGAA GATCTTCAGG ACCCAGGGCC AGGCTCTGGA CAAGTTCGCC 
AAGAAGGACG TCAAGGTGCT GGTTGTGGGC AACCCGGCCA ATACCAATGC CCTGGTCTGC 
TCCTCCTATG CGCCTTCCAT TCCGCGCGAA AATTTCTCGG CTATGACTCG CCTGGATCAG 
AATCGCGCCA CATCCCAGAT CGCCGCCAAA TTGGGTGTGC CAATTTCTGC TGTTAAGAAC 
ATCATTATTT GGGGCAACCA CTCCTCCACC CAGTATCCCG ATGCTGGACA GGCCAAGGTG 
ACCGCCAATG GTACCGTTAA GTCCGTGGTG GATGCGATCA ACGATAATGG CTACCTGCAG 
GGATCCTTCG TGGAAACCGT TCAGAAGCGT GGAGCCGCTG TCATTGCGGC CCGCAAAATG 
TCATCGGCCA TGTCGGCGGC GAAGGCCGCT TGCGATCACA TGCACGACTG GTGGAACGGC 
ACTGCTCCCG GCCAGTTCGT CTCCATGGGT GTCTTCTCCG ACGGCAGCTA CGATTCGCCC 
AAGGATGTGA TCTTCTCGTT CCCCGTCGAG ATCAAGAATA AGCAATGGAA AATCGTCTCT 
GGCCTGACCC TCAGCGATTT CGCTAAGACA AAGTTAAGCG TTACCGGCAA GGAGCTGCAG 
GAGGAGAAGG ACGAGGCTCT GTCCGTGCTG GACTCGAATG TGTCCAACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609394.1
CDS	59..1072
Translation	MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAMPRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPANTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKVTANGTVKSVVDAINDNGYLQGSFVETVQKRGAAVIAARKMSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNKQWKIVSGLTLSDFAKTKLSVTGKELQEEKDEALSVLDSNVSNL

Target ORF information:

RefSeq Version	NM_135550.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malate dehydrogenase 1, transcript variant A (Mdh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135550.2

ORF Insert Sequence:

ATGGCTGAAC CAATTCGTGT TGTGGTGACC GGAGCCGCTG GCCAAATCGC CTACTCCCTG 
CTGTACATGA TTGCCCGCGG CGAGGTGTTC GGCAAGGATC AGCCCATCGT GCTGCACCTG 
CTCGACATTC CGCCCATGGT TGGTGTGCTC GAGGGCGTGG TCATGGAATT GGCCGACTGC 
GCTCTGCCGC TGCTGGTCGA GGTGGTGCCC ACCACCGACC CGGCTGTTGG ATTCAAGGAT 
GTCTCGGCCG CTTTCCTCGT GGGCGCCATG CCCCGCAAGG AGGGAATGGA GCGAAAGGAT 
CTGCTGTCCG CCAACGTGAA GATCTTCAGG ACCCAGGGCC AGGCTCTGGA CAAGTTCGCC 
AAGAAGGACG TCAAGGTGCT GGTTGTGGGC AACCCGGCCA ATACCAATGC CCTGGTCTGC 
TCCTCCTATG CGCCTTCCAT TCCGCGCGAA AATTTCTCGG CTATGACTCG CCTGGATCAG 
AATCGCGCCA CATCCCAGAT CGCCGCCAAA TTGGGTGTGC CAATTTCTGC TGTTAAGAAC 
ATCATTATTT GGGGCAACCA CTCCTCCACC CAGTATCCCG ATGCTGGACA GGCCAAGGTG 
ACCGCCAATG GTACCGTTAA GTCCGTGGTG GATGCGATCA ACGATAATGG CTACCTGCAG 
GGATCCTTCG TGGAAACCGT TCAGAAGCGT GGAGCCGCTG TCATTGCGGC CCGCAAAATG 
TCATCGGCCA TGTCGGCGGC GAAGGCCGCT TGCGATCACA TGCACGACTG GTGGAACGGC 
ACTGCTCCCG GCCAGTTCGT CTCCATGGGT GTCTTCTCCG ACGGCAGCTA CGATTCGCCC 
AAGGATGTGA TCTTCTCGTT CCCCGTCGAG ATCAAGAATA AGCAATGGAA AATCGTCTCT 
GGCCTGACCC TCAGCGATTT CGCTAAGACA AAGTTAAGCG TTACCGGCAA GGAGCTGCAG 
GAGGAGAAGG ACGAGGCTCT GTCCGTGCTG GACTCGAATG TGTCCAACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mdh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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