Mdh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdh1
Entrez Gene ID	24551
Full Name	malate dehydrogenase 1
Synonyms	MDL1,Mdhl,Mor2
General protein information	Preferred Names malate dehydrogenase 1 Names malate dehydrogenase, cytoplasmic malate dehydrogenase, peroxisomal Malate dehydrogenase-like enzyme cytosolic malate dehydrogenase malate dehydrogenase 1, NAD (soluble) malate dehydrogenase, soluble
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316877.1	NP_001303806.1	malate dehydrogenase, peroxisomal isoform Mdh1x
NM_033235.2	NP_150238.1	malate dehydrogenase, cytoplasmic isoform Mdh1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MDH1	XP_531844.1
Bos taurus (cattle)	MDH1	NP_001029800.1
Caenorhabditis elegans (roundworm)	mdh-1	NP_504656.1
Xenopus tropicalis (tropical clawed frog)	mdh1	NP_001006694.1
	MDH1	XP_001084973.1
Arabidopsis thaliana (thale cress)	AT5G43330	NP_199147.1
Pan troglodytes (chimpanzee)	MDH1	NP_001229997.1
Homo sapiens (human)	MDH1	NP_001186040.1
Gallus gallus (chicken)	MDH1	NP_001006395.1
Oryza sativa (rice)	Os10g0478200	NP_001064860.1
Mus musculus (house mouse)	Mdh1	NP_032644.3
Rattus norvegicus (Norway rat)	Mdh1	NP_150238.1
Danio rerio (zebrafish)	mdh1b	NP_956263.1
Drosophila melanogaster (fruit fly)	Mdh1	NP_609394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009510	XP_310186.3
Arabidopsis thaliana (thale cress)	AT1G04410	NP_171936.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats.
Guimar?es Filho A, Cunha RM, Vasconcelos PR, Guimar?es SB
Acta cirurgica brasileira29(6)365-70(2014 Jun)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences.
Ying W
Antioxidants & redox signaling10(2)179-206(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mdh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09092	NM_033235.2 Latest version!	Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa63209	NM_001316877.1 Latest version!	Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdh1 ( NM_033235.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_150238.1 Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa09092
Clone ID Related Accession (Same CDS sequence)	NM_033235.2
Accession Version	NM_033235.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	malate dehydrogenase, cytoplasmic isoform Mdh1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FM081843.1, FN799180.1, FM055335.1, FM085125.1, FM108375.1 and FM086546.1. On Oct 28, 2015 this sequence version replaced NM_033235.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Mdh1) results from translation termination at the upstream UGA stop codon, while the longer isoform (Mdh1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (Mdh1), which is localized in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ215603.1, FQ217819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAGC CAATCAGAGT CCTCGTGACA GGAGCAGCCG GTCAGATTGC ATATTCGCTG 
CTGTACAGCA TTGGAAATGG ATCTGTCTTT GGGAAAGACC AGCCCATCAT TCTTGTGCTG 
TTGGACATCA CCCCCATGAT GGGTGTTCTG GACGGTGTCC TGATGGAGCT GCAAGACTGT 
GCCCTTCCCC TTCTGCAGGA TGTCATTGCA ACAGACAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGACGTGG CTGTCCTTGT GGGCTCCATG CCAAGAAGGG AGGGCATGGA GAGGAAGGAC 
CTACTGAAAG CCAACGTGAA GATCTTCAAA TCCCAGGGCG CAGCCTTGGA GAAGTACGCC 
AAGAAATCAG TTAAGGTCAT TGTTGTGGGG AACCCAGCCA ATACAAACTG CCTGACGGCC 
TCCAAGTCAG CACCATCGAT CCCCAAGGAG AACTTCAGTT GCCTGACTCG ATTGGACCAC 
AACCGAGCAA AATCTCAAAT TGCTCTTAAA CTCGGTGTAA CCGCTGATGA TGTAAAAAAT 
GTCATTATCT GGGGAAATCA TTCATCAACC CAGTATCCAG ATGTCAATCA TGCCAAGGTG 
AAATTGCAAG GAAAAGAAGT TGGTGTGTAT GAAGCCCTCA AAGACGACAG CTGGCTCAAG 
GGAGAGTTCA TCACGACTGT GCAGCAGCGT GGTGCTGCTG TCATCAAGGC TCGGAAGCTG 
TCCAGTGCCA TGTCTGCTGC GAAGGCCATC TCGGACCACA TCAGAGACAT CTGGTTTGGA 
ACCCCGGAGG GCGAGTTCGT GTCGATGGGC GTAATCTCTG ATGGCAACTC CTATGGTGTC 
CCTGATGACC TGCTCTACTC GTTCCCTGTC GTGATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGCCTCC CCATTAACGA CTTCTCCCGT GAGAAGATGG ACCTGACAGC AAAGGAGCTG 
ACCGAGGAAA AGGAAACGGC TTTTGAGTTT CTCTCCTCCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150238.1
CDS	78..1082
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEVAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGAALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAISDHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA

Target ORF information:

RefSeq Version	NM_033235.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033235.2

ORF Insert Sequence:

ATGTCTGAGC CAATCAGAGT CCTCGTGACA GGAGCAGCCG GTCAGATTGC ATATTCGCTG 
CTGTACAGCA TTGGAAATGG ATCTGTCTTT GGGAAAGACC AGCCCATCAT TCTTGTGCTG 
TTGGACATCA CCCCCATGAT GGGTGTTCTG GACGGTGTCC TGATGGAGCT GCAAGACTGT 
GCCCTTCCCC TTCTGCAGGA TGTCATTGCA ACAGACAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGACGTGG CTGTCCTTGT GGGCTCCATG CCAAGAAGGG AGGGCATGGA GAGGAAGGAC 
CTACTGAAAG CCAACGTGAA GATCTTCAAA TCCCAGGGCG CAGCCTTGGA GAAGTACGCC 
AAGAAATCAG TTAAGGTCAT TGTTGTGGGG AACCCAGCCA ATACAAACTG CCTGACGGCC 
TCCAAGTCAG CACCATCGAT CCCCAAGGAG AACTTCAGTT GCCTGACTCG ATTGGACCAC 
AACCGAGCAA AATCTCAAAT TGCTCTTAAA CTCGGTGTAA CCGCTGATGA TGTAAAAAAT 
GTCATTATCT GGGGAAATCA TTCATCAACC CAGTATCCAG ATGTCAATCA TGCCAAGGTG 
AAATTGCAAG GAAAAGAAGT TGGTGTGTAT GAAGCCCTCA AAGACGACAG CTGGCTCAAG 
GGAGAGTTCA TCACGACTGT GCAGCAGCGT GGTGCTGCTG TCATCAAGGC TCGGAAGCTG 
TCCAGTGCCA TGTCTGCTGC GAAGGCCATC TCGGACCACA TCAGAGACAT CTGGTTTGGA 
ACCCCGGAGG GCGAGTTCGT GTCGATGGGC GTAATCTCTG ATGGCAACTC CTATGGTGTC 
CCTGATGACC TGCTCTACTC GTTCCCTGTC GTGATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGCCTCC CCATTAACGA CTTCTCCCGT GAGAAGATGG ACCTGACAGC AAAGGAGCTG 
ACCGAGGAAA AGGAAACGGC TTTTGAGTTT CTCTCCTCCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdh1 ( NM_001316877.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001303806.1 Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63209
Clone ID Related Accession (Same CDS sequence)	NM_001316877.1
Accession Version	NM_001316877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	malate dehydrogenase, peroxisomal isoform Mdh1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FM081843.1, FN799180.1, FM055335.1, FM085125.1, FM108375.1 and FM086546.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Mdh1) results from translation termination at the upstream UGA stop codon, while the longer isoform (Mdh1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (Mdh1x), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ215603.1, FQ217819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAGC CAATCAGAGT CCTCGTGACA GGAGCAGCCG GTCAGATTGC ATATTCGCTG 
CTGTACAGCA TTGGAAATGG ATCTGTCTTT GGGAAAGACC AGCCCATCAT TCTTGTGCTG 
TTGGACATCA CCCCCATGAT GGGTGTTCTG GACGGTGTCC TGATGGAGCT GCAAGACTGT 
GCCCTTCCCC TTCTGCAGGA TGTCATTGCA ACAGACAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGACGTGG CTGTCCTTGT GGGCTCCATG CCAAGAAGGG AGGGCATGGA GAGGAAGGAC 
CTACTGAAAG CCAACGTGAA GATCTTCAAA TCCCAGGGCG CAGCCTTGGA GAAGTACGCC 
AAGAAATCAG TTAAGGTCAT TGTTGTGGGG AACCCAGCCA ATACAAACTG CCTGACGGCC 
TCCAAGTCAG CACCATCGAT CCCCAAGGAG AACTTCAGTT GCCTGACTCG ATTGGACCAC 
AACCGAGCAA AATCTCAAAT TGCTCTTAAA CTCGGTGTAA CCGCTGATGA TGTAAAAAAT 
GTCATTATCT GGGGAAATCA TTCATCAACC CAGTATCCAG ATGTCAATCA TGCCAAGGTG 
AAATTGCAAG GAAAAGAAGT TGGTGTGTAT GAAGCCCTCA AAGACGACAG CTGGCTCAAG 
GGAGAGTTCA TCACGACTGT GCAGCAGCGT GGTGCTGCTG TCATCAAGGC TCGGAAGCTG 
TCCAGTGCCA TGTCTGCTGC GAAGGCCATC TCGGACCACA TCAGAGACAT CTGGTTTGGA 
ACCCCGGAGG GCGAGTTCGT GTCGATGGGC GTAATCTCTG ATGGCAACTC CTATGGTGTC 
CCTGATGACC TGCTCTACTC GTTCCCTGTC GTGATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGCCTCC CCATTAACGA CTTCTCCCGT GAGAAGATGG ACCTGACAGC AAAGGAGCTG 
ACCGAGGAAA AGGAAACGGC TTTTGAGTTT CTCTCCTCCG CATGACTACA CAGTCGTGTT 
GACGTCAGCA GACATCCCAA GGCTGAGGAA TCAAAATGTC GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303806.1
CDS	78..1145
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEVAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGAALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAISDHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA*LHSRVDVSRHPKAEESKCRL

Target ORF information:

RefSeq Version	NM_001316877.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus malate dehydrogenase 1 (Mdh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316877.1

ORF Insert Sequence:

ATGTCTGAGC CAATCAGAGT CCTCGTGACA GGAGCAGCCG GTCAGATTGC ATATTCGCTG 
CTGTACAGCA TTGGAAATGG ATCTGTCTTT GGGAAAGACC AGCCCATCAT TCTTGTGCTG 
TTGGACATCA CCCCCATGAT GGGTGTTCTG GACGGTGTCC TGATGGAGCT GCAAGACTGT 
GCCCTTCCCC TTCTGCAGGA TGTCATTGCA ACAGACAAAG AAGAGGTTGC CTTCAAAGAC 
CTGGACGTGG CTGTCCTTGT GGGCTCCATG CCAAGAAGGG AGGGCATGGA GAGGAAGGAC 
CTACTGAAAG CCAACGTGAA GATCTTCAAA TCCCAGGGCG CAGCCTTGGA GAAGTACGCC 
AAGAAATCAG TTAAGGTCAT TGTTGTGGGG AACCCAGCCA ATACAAACTG CCTGACGGCC 
TCCAAGTCAG CACCATCGAT CCCCAAGGAG AACTTCAGTT GCCTGACTCG ATTGGACCAC 
AACCGAGCAA AATCTCAAAT TGCTCTTAAA CTCGGTGTAA CCGCTGATGA TGTAAAAAAT 
GTCATTATCT GGGGAAATCA TTCATCAACC CAGTATCCAG ATGTCAATCA TGCCAAGGTG 
AAATTGCAAG GAAAAGAAGT TGGTGTGTAT GAAGCCCTCA AAGACGACAG CTGGCTCAAG 
GGAGAGTTCA TCACGACTGT GCAGCAGCGT GGTGCTGCTG TCATCAAGGC TCGGAAGCTG 
TCCAGTGCCA TGTCTGCTGC GAAGGCCATC TCGGACCACA TCAGAGACAT CTGGTTTGGA 
ACCCCGGAGG GCGAGTTCGT GTCGATGGGC GTAATCTCTG ATGGCAACTC CTATGGTGTC 
CCTGATGACC TGCTCTACTC GTTCCCTGTC GTGATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGCCTCC CCATTAACGA CTTCTCCCGT GAGAAGATGG ACCTGACAGC AAAGGAGCTG 
ACCGAGGAAA AGGAAACGGC TTTTGAGTTT CTCTCCTCCG CATGACTACA CAGTCGTGTT 
GACGTCAGCA GACATCCCAA GGCTGAGGAA TCAAAATGTC GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
PLoS genetics10(10)e1004685(2014 Oct)
Stiebler AC,Freitag J,Schink KO,Stehlik T,Tillmann BA,Ast J,B?lker M

Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats.
Acta cirurgica brasileira29(6)365-70(2014 Jun)
Guimar?es Filho A,Cunha RM,Vasconcelos PR,Guimar?es SB

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences.
Antioxidants & redox signaling10(2)179-206(2008 Feb)
Ying W

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Mdh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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