mdh1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mdh1
Entrez Gene ID	448318
Full Name	malate dehydrogenase 1
Synonyms	mdh-s,mdha,mor2
General protein information	Preferred Names malate dehydrogenase 1 Names malate dehydrogenase, cytoplasmic malate dehydrogenase, peroxisomal cytosolic malate dehydrogenase malate dehydrogenase 1, NAD (soluble)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)
Summary	This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316901.1	NP_001303830.1	malate dehydrogenase, peroxisomal isoform mdh1x
NM_001006693.2	NP_001006694.1	malate dehydrogenase, cytoplasmic isoform mdh1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MDH1	XP_531844.1
Bos taurus (cattle)	MDH1	NP_001029800.1
Caenorhabditis elegans (roundworm)	mdh-1	NP_504656.1
Xenopus tropicalis (tropical clawed frog)	mdh1	NP_001006694.1
	MDH1	XP_001084973.1
Arabidopsis thaliana (thale cress)	AT5G43330	NP_199147.1
Pan troglodytes (chimpanzee)	MDH1	NP_001229997.1
Homo sapiens (human)	MDH1	NP_001186040.1
Gallus gallus (chicken)	MDH1	NP_001006395.1
Oryza sativa (rice)	Os10g0478200	NP_001064860.1
Mus musculus (house mouse)	Mdh1	NP_032644.3
Rattus norvegicus (Norway rat)	Mdh1	NP_150238.1
Danio rerio (zebrafish)	mdh1b	NP_956263.1
Drosophila melanogaster (fruit fly)	Mdh1	NP_609394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009510	XP_310186.3
Arabidopsis thaliana (thale cress)	AT1G04410	NP_171936.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mdh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mdh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa02211	NM_001006693.2 Latest version!	Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa47193	NM_001316901.1 Latest version!	Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mdh1 ( NM_001006693.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006694.1 Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa02211
Clone ID Related Accession (Same CDS sequence)	NM_001006693.2
Accession Version	NM_001006693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Xenopus tropicalis(tropical clawed frog)
Product	malate dehydrogenase, cytoplasmic isoform mdh1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL827704.1 and BC075396.1. On Oct 30, 2015 this sequence version replaced NM_001006693.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (mdh1) results from translation termination at the upstream UGA stop codon, while the longer isoform (mdh1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the shorter isoform (mdh1), which is localized in the cytosol. ##Evidence-Data-START## Transcript exon combination :: BC075396.1, CR761081.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGAAC CTGTGAAAGT CCTTGTGACC GGAGCTGCTG GTCAGATTGC TTACTCCCTG 
CTCTATGGTA TTGCTAAAGG CGATGTCTTT GGAAAAGACC AGCCTCTTAT ACTTGTTCTT 
TTGGATATTA CCCCTATGAT GACTGTACTT GAAGGTGTAG TGATGGAGTT GCAGGACTGT 
GCTCTGCCCC TGCTGAAAGA GGTGATTGCT ACCGATAAGG AAGATGTGGC CTTCAAAGAT 
TTGGATGTTG CCATTCTGGT TGGTTCCATG CCAAGAAGGG AGGGCATGGA AAGAAAGGAC 
TTGCTGAAAG CCAATGTAAA GATTTTTAAG TCTCAGGGTG CAGCTCTGAA CAAGTATTCC 
AAGAAGAGTG TTAAGGTTAT TGTTGTGGGG AATCCAGCAA ACACAAATTG CCTGACTGCA 
ATGAAGTCTG CTCCATCCAT CCCTAAAGAG AACTTCAGCT GTCTGACACG CTTAGATCAC 
AACAGAGCCA AAGGACAAAT TGCTCTGAAG TTGAATGTTG CTTCTGATGA TGTAAAGAAT 
GTAATTATCT GGGGTAATCA CTCATCCACT CAGTATCCTG ATGCTTCCCA TGCAACTGTG 
AATCTGCAAG GAAAAGATGT TGGTGCCTTT GAAGCAGTGA AGAATGATGA CTGGCTAAAG 
GGTGAATTTA TCACTACAGT GCAGCAAAGA GGAGCAGCTG TTATTAAGGC CAGAAAGTTG 
TCTAGTGCAA TGTCTGCTGC TAAAGCCATC TGCGATCATG TGAGGGATAT CTGGTTTGGA 
ACCCCTGATG GGCAGTTTGT TTCCATGGGA GTTATTTCGG ATGGAAACTC TTATGGTATA 
CCTGAAGACC TGATGTATTC ATTCCCTCTT ACCATCAAGA ATAAGACCTG GAAAATAGTG 
GAAGGCCTGC CTATTAATGA CTTTTCCCGT GAGAAAATGG ATCTCACAGC TAAAGAACTT 
CATGAAGAAA AAGAAACTGC ACTTGAATTT CTGTCCTGCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006694.1
CDS	186..1190
Translation	MPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNLQGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLSCE

Target ORF information:

RefSeq Version	NM_001006693.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006693.2

ORF Insert Sequence:

ATGCCTGAAC CTGTGAAAGT CCTTGTGACC GGAGCTGCTG GTCAGATTGC TTACTCCCTG 
CTCTATGGTA TTGCTAAAGG CGATGTCTTT GGAAAAGACC AGCCTCTTAT ACTTGTTCTT 
TTGGATATTA CCCCTATGAT GACTGTACTT GAAGGTGTAG TGATGGAGTT GCAGGACTGT 
GCTCTGCCCC TGCTGAAAGA GGTGATTGCT ACCGATAAGG AAGATGTGGC CTTCAAAGAT 
TTGGATGTTG CCATTCTGGT TGGTTCCATG CCAAGAAGGG AGGGCATGGA AAGAAAGGAC 
TTGCTGAAAG CCAATGTAAA GATTTTTAAG TCTCAGGGTG CAGCTCTGAA CAAGTATTCC 
AAGAAGAGTG TTAAGGTTAT TGTTGTGGGG AATCCAGCAA ACACAAATTG CCTGACTGCA 
ATGAAGTCTG CTCCATCCAT CCCTAAAGAG AACTTCAGCT GTCTGACACG CTTAGATCAC 
AACAGAGCCA AAGGACAAAT TGCTCTGAAG TTGAATGTTG CTTCTGATGA TGTAAAGAAT 
GTAATTATCT GGGGTAATCA CTCATCCACT CAGTATCCTG ATGCTTCCCA TGCAACTGTG 
AATCTGCAAG GAAAAGATGT TGGTGCCTTT GAAGCAGTGA AGAATGATGA CTGGCTAAAG 
GGTGAATTTA TCACTACAGT GCAGCAAAGA GGAGCAGCTG TTATTAAGGC CAGAAAGTTG 
TCTAGTGCAA TGTCTGCTGC TAAAGCCATC TGCGATCATG TGAGGGATAT CTGGTTTGGA 
ACCCCTGATG GGCAGTTTGT TTCCATGGGA GTTATTTCGG ATGGAAACTC TTATGGTATA 
CCTGAAGACC TGATGTATTC ATTCCCTCTT ACCATCAAGA ATAAGACCTG GAAAATAGTG 
GAAGGCCTGC CTATTAATGA CTTTTCCCGT GAGAAAATGG ATCTCACAGC TAAAGAACTT 
CATGAAGAAA AAGAAACTGC ACTTGAATTT CTGTCCTGCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdh1 ( NM_001316901.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001303830.1 Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa47193
Clone ID Related Accession (Same CDS sequence)	NM_001316901.1
Accession Version	NM_001316901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	malate dehydrogenase, peroxisomal isoform mdh1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL827704.1 and BC075396.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (mdh1) results from translation termination at the upstream UGA stop codon, while the longer isoform (mdh1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the longer, C-terminally extended isoform (mdh1x), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: BC075396.1, CR761081.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTGAAC CTGTGAAAGT CCTTGTGACC GGAGCTGCTG GTCAGATTGC TTACTCCCTG 
CTCTATGGTA TTGCTAAAGG CGATGTCTTT GGAAAAGACC AGCCTCTTAT ACTTGTTCTT 
TTGGATATTA CCCCTATGAT GACTGTACTT GAAGGTGTAG TGATGGAGTT GCAGGACTGT 
GCTCTGCCCC TGCTGAAAGA GGTGATTGCT ACCGATAAGG AAGATGTGGC CTTCAAAGAT 
TTGGATGTTG CCATTCTGGT TGGTTCCATG CCAAGAAGGG AGGGCATGGA AAGAAAGGAC 
TTGCTGAAAG CCAATGTAAA GATTTTTAAG TCTCAGGGTG CAGCTCTGAA CAAGTATTCC 
AAGAAGAGTG TTAAGGTTAT TGTTGTGGGG AATCCAGCAA ACACAAATTG CCTGACTGCA 
ATGAAGTCTG CTCCATCCAT CCCTAAAGAG AACTTCAGCT GTCTGACACG CTTAGATCAC 
AACAGAGCCA AAGGACAAAT TGCTCTGAAG TTGAATGTTG CTTCTGATGA TGTAAAGAAT 
GTAATTATCT GGGGTAATCA CTCATCCACT CAGTATCCTG ATGCTTCCCA TGCAACTGTG 
AATCTGCAAG GAAAAGATGT TGGTGCCTTT GAAGCAGTGA AGAATGATGA CTGGCTAAAG 
GGTGAATTTA TCACTACAGT GCAGCAAAGA GGAGCAGCTG TTATTAAGGC CAGAAAGTTG 
TCTAGTGCAA TGTCTGCTGC TAAAGCCATC TGCGATCATG TGAGGGATAT CTGGTTTGGA 
ACCCCTGATG GGCAGTTTGT TTCCATGGGA GTTATTTCGG ATGGAAACTC TTATGGTATA 
CCTGAAGACC TGATGTATTC ATTCCCTCTT ACCATCAAGA ATAAGACCTG GAAAATAGTG 
GAAGGCCTGC CTATTAATGA CTTTTCCCGT GAGAAAATGG ATCTCACAGC TAAAGAACTT 
CATGAAGAAA AAGAAACTGC ACTTGAATTT CTGTCCTGCG AATGACTAGT GACAGATTCC 
ACAACGCACT TAACCCCTGA GAAAATGAAA AGTTCTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303830.1
CDS	186..1247
Translation	MPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNLQGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLSCE*LVTDSTTHLTPEKMKSSL

Target ORF information:

RefSeq Version	NM_001316901.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis malate dehydrogenase 1 (mdh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316901.1

ORF Insert Sequence:

ATGCCTGAAC CTGTGAAAGT CCTTGTGACC GGAGCTGCTG GTCAGATTGC TTACTCCCTG 
CTCTATGGTA TTGCTAAAGG CGATGTCTTT GGAAAAGACC AGCCTCTTAT ACTTGTTCTT 
TTGGATATTA CCCCTATGAT GACTGTACTT GAAGGTGTAG TGATGGAGTT GCAGGACTGT 
GCTCTGCCCC TGCTGAAAGA GGTGATTGCT ACCGATAAGG AAGATGTGGC CTTCAAAGAT 
TTGGATGTTG CCATTCTGGT TGGTTCCATG CCAAGAAGGG AGGGCATGGA AAGAAAGGAC 
TTGCTGAAAG CCAATGTAAA GATTTTTAAG TCTCAGGGTG CAGCTCTGAA CAAGTATTCC 
AAGAAGAGTG TTAAGGTTAT TGTTGTGGGG AATCCAGCAA ACACAAATTG CCTGACTGCA 
ATGAAGTCTG CTCCATCCAT CCCTAAAGAG AACTTCAGCT GTCTGACACG CTTAGATCAC 
AACAGAGCCA AAGGACAAAT TGCTCTGAAG TTGAATGTTG CTTCTGATGA TGTAAAGAAT 
GTAATTATCT GGGGTAATCA CTCATCCACT CAGTATCCTG ATGCTTCCCA TGCAACTGTG 
AATCTGCAAG GAAAAGATGT TGGTGCCTTT GAAGCAGTGA AGAATGATGA CTGGCTAAAG 
GGTGAATTTA TCACTACAGT GCAGCAAAGA GGAGCAGCTG TTATTAAGGC CAGAAAGTTG 
TCTAGTGCAA TGTCTGCTGC TAAAGCCATC TGCGATCATG TGAGGGATAT CTGGTTTGGA 
ACCCCTGATG GGCAGTTTGT TTCCATGGGA GTTATTTCGG ATGGAAACTC TTATGGTATA 
CCTGAAGACC TGATGTATTC ATTCCCTCTT ACCATCAAGA ATAAGACCTG GAAAATAGTG 
GAAGGCCTGC CTATTAATGA CTTTTCCCGT GAGAAAATGG ATCTCACAGC TAAAGAACTT 
CATGAAGAAA AAGAAACTGC ACTTGAATTT CTGTCCTGCG AATGACTAGT GACAGATTCC 
ACAACGCACT TAACCCCTGA GAAAATGAAA AGTTCTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
PLoS genetics10(10)e1004685(2014 Oct)
Stiebler AC,Freitag J,Schink KO,Stehlik T,Tillmann BA,Ast J,B?lker M

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

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mdh1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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