NCU07870 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07870
Entrez Gene ID	3879279
Full Name	DNA polymerase alpha catalytic subunit
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958038.3	XP_963131.2	DNA polymerase alpha catalytic subunit

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07870 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07870 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01768	XM_958038.3 Latest version!	Neurospora crassa OR74A DNA polymerase alpha catalytic subunit (NCU07870), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07870 ( XM_958038.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_963131.2 Neurospora crassa OR74A DNA polymerase alpha catalytic subunit (NCU07870), mRNA.

Price & Availability↑

CloneID	ONf01768
Clone ID Related Accession (Same CDS sequence)	XM_958038.3
Accession Version	XM_958038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_958038.2.

ATGTCCAGAC GAGTAGCCAA GCGAGACAAG TACGCTGAGC TGCGCGAACT GCGCCAGTCC 
GGCAAGAAGA AGACCTACGA CGTCGGCGAG ATCGACGAGC TCTACGAAGA AGTCGACGAG 
AACCAGTACA AGAAAATCGT CAGAGACCGC TTGAACGAGG ACGATTTCGT GGTTGACGAC 
AATGGCGAGG GTTATGCCGA CGACGGCCGC GAAGAATGGG ACCGTCTCCC CCAGTACCAC 
AGCGACAGCG AGGAGGAATT AGGCCTACCC GGCAGCAAGC CGCGCAAGTC CAAGAAACAA 
AAGGAACATG AAGATGCGAA ACGAGATGCC AACGACAGAG ACATCACCGA ATACTTCACG 
AAAGGCGCCG TCAAAGCCCC ACCAAAACAA AAGGTCATCA AAACAGAAGA AGATGAAAAG 
TTCTTGGACG ACCTCATCGA CCAGGTCACC GCACAAATCC CCGTCCCGGT CACACGAGTA 
TCCAAGAAGC GAGATCGATC AGTCGAGAGG AGAAAGGTCC GGGTGCTCTC CCCGGTACGG 
GAACCTCGGC CACCCATGGC AAAGAGAGCC AAGACAGTCG ATGATCGGGC ATCATCCCCA 
GCAGGAGACG ACGACCTTCT ACCAGACGAC GACTACCTGC CTGCTATGGA CGACGAACCT 
GCACCAGTCT CCGATTATCT TATGAGCGAC GCCATCATGC CCTCCTCACC GGCCGCCAAA 
GTAGCAACAC GGAGGACGTT TGGCAGGCCG GAACCCAAAA AGAATGAGGA TGATGAGGAC 
GAAGATGAGG ACATGATGGA GGTTGCCCAT ACTGGAGCGG TAACAGCCGC CAGCGTCAAC 
ATATCAGCAT CCCGTCAAAT CAAAAAGATT CTCAAGACAG AGCCCGTCCC GGCCAGTTCG 
TCACCCGCAA GGGCACCAGC GCCAGAGGTC GACGCTGCTT CCTGGAACGA GATCAATCAG 
AAGCTCAATG TCGTATCCAT GTCACAAAGC GAGGGCAGGA GCGTTGGCAA GATTGACTAC 
AAGGACGCTA TCGAAGAAGA CGGCAGCTTG AACATGTTCT GGACAGACTA CACAGAGGTC 
AACGGCAGTT TGTGCTTGTT TGGCAAGGTC CTAAACAAAA AGACCAAAAC CTACGTCAGC 
TGCTTTGTCA AGGTTGACAA CATTCTCCGG AAGCTTTTCT TCCTGCCGCG CAAGTACCGT 
GTGCGAGGTG GCGAGGAAAC AACAGAAGAG GTGGAAATGA TGGACGTCTA CAACGAGGTT 
GATGAAATCA TGACCAAGAT GAATGTCGGC ATGCACAAGA TCAAGGCTTG CACCCGCAAG 
TACGCATTCG AGCTTCCCGA CGTTCCCAAG GAGAGCCAGT ATTTGAAGCT CCTCTACCCA 
TATACCAAGC CTGCGATGGA ACCCACCCAC AAAGGCGAAA CCTTCTCCCA CGTCTTTGGT 
ACCAATACCG CTCTGTTCGA GCAGTTTGTG CTCTGGAAGA ACATCATGGG CCCCTGCTGG 
CTCAAGATCG AGGATGCAGA CTTCACTTCC TTGAGGAATG CTTCGCACTG CAAGCTGGAG 
GTCCTGGTAG AACATCCCAA CATGGTGTCG GTCCCGGATA ACGCCGAGAG TATTGAGCCT 
CCTCCTTTGA CGTTGTTAAG CCTGGCTATG CGCACAGCGT TCAACCCTAA GGATAATAAG 
CAGGAGATTC TCGCGGTTAG CGGGAGAGTC TACCTTGATG TGTCGCTCAG TGACACCACT 
CCCCCAGACA AGCTTGCTTC TCGTAGTTTC ACCGCTGTTA GACCTTACGG TAGCGCATTT 
CCTATCGGCT TCGAGACGCT TGCCAAGGAA AGAAACAGAG GTGTGCTCAA GCTCTTCAAG 
CAGGAACACG AGATCTTGAA TTTCTTGCTC GCACAGATCG ATGTTGTCGA TCCAGACGTT 
ATCCTTGGCC ACCAGCTAGA AGGTGTTGAC TACAGCATCT TGCTGAACCG GCTGCACGAG 
AAAAAGACAC ATCAATGGTC TCGTTTGGGC AGACTCAGGC GCTCTCAGTG GCCCTCGTCC 
ATTGTTAAGA TGGGTGGCAA TGTATTTGCC GAACGTCAGA TAATGTCTGG CCGTCTGCTA 
TGCGATCTTG CTAACGATGC CGGTAAATCA GTAATGACCA AGTGCCAGAC CTGGTCCTTG 
ACCGAGATGT GCAGTCTGTA TCTGGGAGGC GAAAGTCGTA GGAGGGATAT CGACAACGAA 
GTCGCCCTGA AGACGTGGGC TAACGCTGAC AAACATGGTT TGATGGACTA CATCTCCCAC 
GCCGAGACTG ACACTTACTT CATCGCGGCT CTTGCACTCA GGACTCAAAT TCTTCCTCTT 
ACCAAGGTTC TCACCAACCT GGCTGGTAAC TCGTGGGCAA GAACACTTAC CGGTACGCGT 
GCTGAGCGGA ACGAGTACAT TTTGCTTCAC GAATTCCACC GCAACAAATA TGTCGTCCCG 
GACAAGCAGA CCTTCAAGGG TCGGCAACGC ATCGAGGAGG AAAACGCAGA AGAGGAAGGC 
GGAGAAGGAG GAAAGAAGAA GGACAAATAC AAGGGTGGTT TGGTTTTCGA GCCAGAAAAG 
GGTCTTTATG ACAAGTTCGT TCTCGTCATG GACTTCAACT CCCTTTACCC TTCCATCATC 
CAAGAGTACA ACATTTGCTT TACCACAGTT GATCGAACAT CACTGTCCGA GGACGATGAT 
GCTGTTCCCG AGGTTCCCAA GGAGCAGGCG CAGGGCATTC TTCCCAAGCT GATTGCCACA 
CTCGTTAGCC GACGTAGGCA GGTCAAGTCT CTGATGAAAG ACAAGAATGC CACACCTGAA 
GAGCTCGCCA CCTGGGATAT CAAGCAGCTC GCCTTGAAGT TGACTGCCAA CTCCATGTAC 
GGCTGTCTTG GTTATACCAA GTCGCGCTTC TACGCCCGTC CTCTTGCCGT GCTCACCACC 
TATAAGGGTC GTGAGATTCT GCGGAGCACC AAAGAATTGG CGGAGAGCAA CTCGCTCCAG 
GTCATCTACG GTGACACGGA TTCCGTCATG ATCAACGCGA ACAAGGACAA CGTTGCTGAA 
GCCCTGCGTG TCGGCCACGA GTTCAAGAAG GCCGTCAACG AGCGGTACCG CCTCCTTGAG 
ATTGATATCG ACAACGTCTT CCGGCGCATC CTCCTCCAAG CCAAGAAGAA GTATGCTGCC 
ATCAACATGG TCGAGGTCAA CGGCAAGTGG GTTGAGAAAA TGGAGGTCAA AGGTCTCGAT 
ATGAAGCGTC GTGAATACTG CGGGCTATCC AAGGAGATCT CCAACCGTAT CCTCACCGAG 
ATCCTCTCGG GAGACGATAC TGAGGTGTCT GTCCAACGCA TCCACGAGTA CCTTCGTGAC 
ATATCTGCCA AGATGCGCGA GGGTGCCGTC CCTGTGCAAA AGTACATCAT CTCGACTCAG 
CTTGGTAAGG CGCCCAAGGA TTATCCCAAT GCCGACTCGA TGCCGCAGGT CCAAGTCGCC 
CTTCGCGAGT TGGCCAAGGG CAAGACCATC CGTAAGGGCG ACGTCATCTC GTACATCATC 
ACAGGCGACA GCAAGACCAC CTCAGAAGCC GTCGCCAAGC GTGCCTACAC CCCTCAAGAC 
GTGCTCAAGG CCGAATCTGG CCTGTCTCCG GACGTAGAAT GGTACATCGG CAAGCAAATA 
TTCCCTCCAG TCGAGCGTCT CTGCGCCAAC ATTGTCGGCA CGTCGACCTC CCAACTCGCA 
GAAAACCTCG GTCTCGACGT CCGCCGCTAC TCTTCCAACA CCAACAACAA CTCCTCAGGC 
CCACAAGACC TCGAAATCCA CCCTCTCGAG TCCCAAATCC CCGACTCCCT ACGCTTCCAG 
TCCTGTGCGC GCCTCTCCCT CCGCTGTCGC AAGTGCAAAA CCTCGCACCC CTTCGAGGGC 
CTCGCTTCCC CATCATCCGT CAACAACGTC ACGCCCTCCG GCATCCAATG CCCCTCATGC 
CAATACGTCC TCCCCATGCT CAGCGTCGTC GCCCAAGTTG AAGCCGCCGT CCGCGCCCAA 
ACAGCACGCT ACTACGAAGG CTGGCTCGTC TGCGACGACT CCTCCTGCGG CAACCGCACG 
CGCCAGATCT CCGTTTATGG CACCCGCTGT CTCGGATCCA AGGGTCTGGC GCGCGGCGAG 
TGTGGCGGCC GGATGCGGTA TGAGTACACG GAACGGGACA TCTACAACCA GCTGGTGTAC 
TTTGCTAGTC TGTGGGACGT GGATAAGGCG AAGAGTAAGG CCAAGGAGAA CAATGATGAA 
GATGTGGATG CTGCTAAGCT AAGTGCAGAG GAGAAGGAGA GGGTCGCCAT CATGGCGGAA 
CATAATCGTG TTAGGTTTGG TACGGTCAAG GGCGTGGTGG AAAAGTATTT GGATAAATGT 
GGACGGAGAT GGGTGGCGAT GGATACGCTT TTTGCCAAGT TGGGGTTTGT TCCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_963131.2
CDS	176..4615
Translation	MSRRVAKRDKYAELRELRQSGKKKTYDVGEIDELYEEVDENQYKKIVRDRLNEDDFVVDDNGEGYADDGREEWDRLPQYHSDSEEELGLPGSKPRKSKKQKEHEDAKRDANDRDITEYFTKGAVKAPPKQKVIKTEEDEKFLDDLIDQVTAQIPVPVTRVSKKRDRSVERRKVRVLSPVREPRPPMAKRAKTVDDRASSPAGDDDLLPDDDYLPAMDDEPAPVSDYLMSDAIMPSSPAAKVATRRTFGRPEPKKNEDDEDEDEDMMEVAHTGAVTAASVNISASRQIKKILKTEPVPASSSPARAPAPEVDAASWNEINQKLNVVSMSQSEGRSVGKIDYKDAIEEDGSLNMFWTDYTEVNGSLCLFGKVLNKKTKTYVSCFVKVDNILRKLFFLPRKYRVRGGEETTEEVEMMDVYNEVDEIMTKMNVGMHKIKACTRKYAFELPDVPKESQYLKLLYPYTKPAMEPTHKGETFSHVFGTNTALFEQFVLWKNIMGPCWLKIEDADFTSLRNASHCKLEVLVEHPNMVSVPDNAESIEPPPLTLLSLAMRTAFNPKDNKQEILAVSGRVYLDVSLSDTTPPDKLASRSFTAVRPYGSAFPIGFETLAKERNRGVLKLFKQEHEILNFLLAQIDVVDPDVILGHQLEGVDYSILLNRLHEKKTHQWSRLGRLRRSQWPSSIVKMGGNVFAERQIMSGRLLCDLANDAGKSVMTKCQTWSLTEMCSLYLGGESRRRDIDNEVALKTWANADKHGLMDYISHAETDTYFIAALALRTQILPLTKVLTNLAGNSWARTLTGTRAERNEYILLHEFHRNKYVVPDKQTFKGRQRIEEENAEEEGGEGGKKKDKYKGGLVFEPEKGLYDKFVLVMDFNSLYPSIIQEYNICFTTVDRTSLSEDDDAVPEVPKEQAQGILPKLIATLVSRRRQVKSLMKDKNATPEELATWDIKQLALKLTANSMYGCLGYTKSRFYARPLAVLTTYKGREILRSTKELAESNSLQVIYGDTDSVMINANKDNVAEALRVGHEFKKAVNERYRLLEIDIDNVFRRILLQAKKKYAAINMVEVNGKWVEKMEVKGLDMKRREYCGLSKEISNRILTEILSGDDTEVSVQRIHEYLRDISAKMREGAVPVQKYIISTQLGKAPKDYPNADSMPQVQVALRELAKGKTIRKGDVISYIITGDSKTTSEAVAKRAYTPQDVLKAESGLSPDVEWYIGKQIFPPVERLCANIVGTSTSQLAENLGLDVRRYSSNTNNNSSGPQDLEIHPLESQIPDSLRFQSCARLSLRCRKCKTSHPFEGLASPSSVNNVTPSGIQCPSCQYVLPMLSVVAQVEAAVRAQTARYYEGWLVCDDSSCGNRTRQISVYGTRCLGSKGLARGECGGRMRYEYTERDIYNQLVYFASLWDVDKAKSKAKENNDEDVDAAKLSAEEKERVAIMAEHNRVRFGTVKGVVEKYLDKCGRRWVAMDTLFAKLGFVPQ

Target ORF information:

RefSeq Version	XM_958038.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA polymerase alpha catalytic subunit (NCU07870), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958038.3

ORF Insert Sequence:

ATGTCCAGAC GAGTAGCCAA GCGAGACAAG TACGCTGAGC TGCGCGAACT GCGCCAGTCC 
GGCAAGAAGA AGACCTACGA CGTCGGCGAG ATCGACGAGC TCTACGAAGA AGTCGACGAG 
AACCAGTACA AGAAAATCGT CAGAGACCGC TTGAACGAGG ACGATTTCGT GGTTGACGAC 
AATGGCGAGG GTTATGCCGA CGACGGCCGC GAAGAATGGG ACCGTCTCCC CCAGTACCAC 
AGCGACAGCG AGGAGGAATT AGGCCTACCC GGCAGCAAGC CGCGCAAGTC CAAGAAACAA 
AAGGAACATG AAGATGCGAA ACGAGATGCC AACGACAGAG ACATCACCGA ATACTTCACG 
AAAGGCGCCG TCAAAGCCCC ACCAAAACAA AAGGTCATCA AAACAGAAGA AGATGAAAAG 
TTCTTGGACG ACCTCATCGA CCAGGTCACC GCACAAATCC CCGTCCCGGT CACACGAGTA 
TCCAAGAAGC GAGATCGATC AGTCGAGAGG AGAAAGGTCC GGGTGCTCTC CCCGGTACGG 
GAACCTCGGC CACCCATGGC AAAGAGAGCC AAGACAGTCG ATGATCGGGC ATCATCCCCA 
GCAGGAGACG ACGACCTTCT ACCAGACGAC GACTACCTGC CTGCTATGGA CGACGAACCT 
GCACCAGTCT CCGATTATCT TATGAGCGAC GCCATCATGC CCTCCTCACC GGCCGCCAAA 
GTAGCAACAC GGAGGACGTT TGGCAGGCCG GAACCCAAAA AGAATGAGGA TGATGAGGAC 
GAAGATGAGG ACATGATGGA GGTTGCCCAT ACTGGAGCGG TAACAGCCGC CAGCGTCAAC 
ATATCAGCAT CCCGTCAAAT CAAAAAGATT CTCAAGACAG AGCCCGTCCC GGCCAGTTCG 
TCACCCGCAA GGGCACCAGC GCCAGAGGTC GACGCTGCTT CCTGGAACGA GATCAATCAG 
AAGCTCAATG TCGTATCCAT GTCACAAAGC GAGGGCAGGA GCGTTGGCAA GATTGACTAC 
AAGGACGCTA TCGAAGAAGA CGGCAGCTTG AACATGTTCT GGACAGACTA CACAGAGGTC 
AACGGCAGTT TGTGCTTGTT TGGCAAGGTC CTAAACAAAA AGACCAAAAC CTACGTCAGC 
TGCTTTGTCA AGGTTGACAA CATTCTCCGG AAGCTTTTCT TCCTGCCGCG CAAGTACCGT 
GTGCGAGGTG GCGAGGAAAC AACAGAAGAG GTGGAAATGA TGGACGTCTA CAACGAGGTT 
GATGAAATCA TGACCAAGAT GAATGTCGGC ATGCACAAGA TCAAGGCTTG CACCCGCAAG 
TACGCATTCG AGCTTCCCGA CGTTCCCAAG GAGAGCCAGT ATTTGAAGCT CCTCTACCCA 
TATACCAAGC CTGCGATGGA ACCCACCCAC AAAGGCGAAA CCTTCTCCCA CGTCTTTGGT 
ACCAATACCG CTCTGTTCGA GCAGTTTGTG CTCTGGAAGA ACATCATGGG CCCCTGCTGG 
CTCAAGATCG AGGATGCAGA CTTCACTTCC TTGAGGAATG CTTCGCACTG CAAGCTGGAG 
GTCCTGGTAG AACATCCCAA CATGGTGTCG GTCCCGGATA ACGCCGAGAG TATTGAGCCT 
CCTCCTTTGA CGTTGTTAAG CCTGGCTATG CGCACAGCGT TCAACCCTAA GGATAATAAG 
CAGGAGATTC TCGCGGTTAG CGGGAGAGTC TACCTTGATG TGTCGCTCAG TGACACCACT 
CCCCCAGACA AGCTTGCTTC TCGTAGTTTC ACCGCTGTTA GACCTTACGG TAGCGCATTT 
CCTATCGGCT TCGAGACGCT TGCCAAGGAA AGAAACAGAG GTGTGCTCAA GCTCTTCAAG 
CAGGAACACG AGATCTTGAA TTTCTTGCTC GCACAGATCG ATGTTGTCGA TCCAGACGTT 
ATCCTTGGCC ACCAGCTAGA AGGTGTTGAC TACAGCATCT TGCTGAACCG GCTGCACGAG 
AAAAAGACAC ATCAATGGTC TCGTTTGGGC AGACTCAGGC GCTCTCAGTG GCCCTCGTCC 
ATTGTTAAGA TGGGTGGCAA TGTATTTGCC GAACGTCAGA TAATGTCTGG CCGTCTGCTA 
TGCGATCTTG CTAACGATGC CGGTAAATCA GTAATGACCA AGTGCCAGAC CTGGTCCTTG 
ACCGAGATGT GCAGTCTGTA TCTGGGAGGC GAAAGTCGTA GGAGGGATAT CGACAACGAA 
GTCGCCCTGA AGACGTGGGC TAACGCTGAC AAACATGGTT TGATGGACTA CATCTCCCAC 
GCCGAGACTG ACACTTACTT CATCGCGGCT CTTGCACTCA GGACTCAAAT TCTTCCTCTT 
ACCAAGGTTC TCACCAACCT GGCTGGTAAC TCGTGGGCAA GAACACTTAC CGGTACGCGT 
GCTGAGCGGA ACGAGTACAT TTTGCTTCAC GAATTCCACC GCAACAAATA TGTCGTCCCG 
GACAAGCAGA CCTTCAAGGG TCGGCAACGC ATCGAGGAGG AAAACGCAGA AGAGGAAGGC 
GGAGAAGGAG GAAAGAAGAA GGACAAATAC AAGGGTGGTT TGGTTTTCGA GCCAGAAAAG 
GGTCTTTATG ACAAGTTCGT TCTCGTCATG GACTTCAACT CCCTTTACCC TTCCATCATC 
CAAGAGTACA ACATTTGCTT TACCACAGTT GATCGAACAT CACTGTCCGA GGACGATGAT 
GCTGTTCCCG AGGTTCCCAA GGAGCAGGCG CAGGGCATTC TTCCCAAGCT GATTGCCACA 
CTCGTTAGCC GACGTAGGCA GGTCAAGTCT CTGATGAAAG ACAAGAATGC CACACCTGAA 
GAGCTCGCCA CCTGGGATAT CAAGCAGCTC GCCTTGAAGT TGACTGCCAA CTCCATGTAC 
GGCTGTCTTG GTTATACCAA GTCGCGCTTC TACGCCCGTC CTCTTGCCGT GCTCACCACC 
TATAAGGGTC GTGAGATTCT GCGGAGCACC AAAGAATTGG CGGAGAGCAA CTCGCTCCAG 
GTCATCTACG GTGACACGGA TTCCGTCATG ATCAACGCGA ACAAGGACAA CGTTGCTGAA 
GCCCTGCGTG TCGGCCACGA GTTCAAGAAG GCCGTCAACG AGCGGTACCG CCTCCTTGAG 
ATTGATATCG ACAACGTCTT CCGGCGCATC CTCCTCCAAG CCAAGAAGAA GTATGCTGCC 
ATCAACATGG TCGAGGTCAA CGGCAAGTGG GTTGAGAAAA TGGAGGTCAA AGGTCTCGAT 
ATGAAGCGTC GTGAATACTG CGGGCTATCC AAGGAGATCT CCAACCGTAT CCTCACCGAG 
ATCCTCTCGG GAGACGATAC TGAGGTGTCT GTCCAACGCA TCCACGAGTA CCTTCGTGAC 
ATATCTGCCA AGATGCGCGA GGGTGCCGTC CCTGTGCAAA AGTACATCAT CTCGACTCAG 
CTTGGTAAGG CGCCCAAGGA TTATCCCAAT GCCGACTCGA TGCCGCAGGT CCAAGTCGCC 
CTTCGCGAGT TGGCCAAGGG CAAGACCATC CGTAAGGGCG ACGTCATCTC GTACATCATC 
ACAGGCGACA GCAAGACCAC CTCAGAAGCC GTCGCCAAGC GTGCCTACAC CCCTCAAGAC 
GTGCTCAAGG CCGAATCTGG CCTGTCTCCG GACGTAGAAT GGTACATCGG CAAGCAAATA 
TTCCCTCCAG TCGAGCGTCT CTGCGCCAAC ATTGTCGGCA CGTCGACCTC CCAACTCGCA 
GAAAACCTCG GTCTCGACGT CCGCCGCTAC TCTTCCAACA CCAACAACAA CTCCTCAGGC 
CCACAAGACC TCGAAATCCA CCCTCTCGAG TCCCAAATCC CCGACTCCCT ACGCTTCCAG 
TCCTGTGCGC GCCTCTCCCT CCGCTGTCGC AAGTGCAAAA CCTCGCACCC CTTCGAGGGC 
CTCGCTTCCC CATCATCCGT CAACAACGTC ACGCCCTCCG GCATCCAATG CCCCTCATGC 
CAATACGTCC TCCCCATGCT CAGCGTCGTC GCCCAAGTTG AAGCCGCCGT CCGCGCCCAA 
ACAGCACGCT ACTACGAAGG CTGGCTCGTC TGCGACGACT CCTCCTGCGG CAACCGCACG 
CGCCAGATCT CCGTTTATGG CACCCGCTGT CTCGGATCCA AGGGTCTGGC GCGCGGCGAG 
TGTGGCGGCC GGATGCGGTA TGAGTACACG GAACGGGACA TCTACAACCA GCTGGTGTAC 
TTTGCTAGTC TGTGGGACGT GGATAAGGCG AAGAGTAAGG CCAAGGAGAA CAATGATGAA 
GATGTGGATG CTGCTAAGCT AAGTGCAGAG GAGAAGGAGA GGGTCGCCAT CATGGCGGAA 
CATAATCGTG TTAGGTTTGG TACGGTCAAG GGCGTGGTGG AAAAGTATTT GGATAAATGT 
GGACGGAGAT GGGTGGCGAT GGATACGCTT TTTGCCAAGT TGGGGTTTGT TCCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07870 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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