NCU03877 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03877
Entrez Gene ID	3872688
Full Name	C-1-tetrahydrofolate synthase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396576.1	XP_011394878.1	C-1-tetrahydrofolate synthase
XM_011396577.1	XP_011394879.1	C-1-tetrahydrofolate synthase, variant

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Neurospora crassa	NCU03877	XP_956550.1
	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03877 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03877 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10094	XM_011396577.1 Latest version!	Neurospora crassa OR74A C-1-tetrahydrofolate synthase, variant (NCU03877), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ONf10093	XM_011396576.1 Latest version!	Neurospora crassa OR74A C-1-tetrahydrofolate synthase (NCU03877), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03877 ( XM_011396577.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394879.1 Neurospora crassa OR74A C-1-tetrahydrofolate synthase, variant (NCU03877), mRNA.

Price & Availability↑

CloneID	ONf10094
Clone ID Related Accession (Same CDS sequence)	XM_011396577.1
Accession Version	XM_011396577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	C-1-tetrahydrofolate synthase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

ATGGCCTCCA AGATCGACGG CACCGCGCTG GCCAAGCGCA TCCGCGACGG TCTCCGCGCT 
ACCATCGACG AGACCAAGAA GACCAACCCG CGCTTCCGCC CCTGCCTCAA GATCATCCAA 
GCTACCTATG TGCGCATGAA GTTGAAGGCT GCTGAGGAGT GCGGTATCGA GGCCGAGCTG 
ATCAAGTTCG ACGAGAATGC CACCGAGGCC GAGCTTGTTG CCAAGGTGGT GCAGCTCAAC 
AACGACTCGA CCGTCCATGG CATGCTCGTC CAGTTGCCCC TCCCCAAGCA CATTTCCGAG 
TATGCTGTCA CATCGGCCGT TGCCGACGAG AAGGATGTCG ACGGTTTCGG CACCCTCAAC 
ATCGGCGAGC TTGCCAAGCG CGGCGGCCAC CCTTATTTCA TCCCCTGCAC CCCCAAGGGT 
GTCATGGCTC TTCTTGCCGA GGCTGGTGTT GAGCTCAAGG GAAAGAACGC TGTTGTCCTC 
GGTCGTAGCG ATATCGTGGG CAGCCCTGTC AGCTACCTCC TCAAGAACGC CGACGCCACC 
GTCACCGTCT GCCACTCCAA GACCGCCGAT CTCCAGCAGT ATCTCAAGGA GGCCGACGTT 
GTTGTTGCTG CCATTGGCCA GCCTAACTTT GTCAAGGGCG AGTGGCTCAA GAAGGGTGCC 
GTTGTCATTG ATGTCGGTAC CAATTACGTT CCCGACGATA CAAAGAAGTC TGGCGTCCGC 
CTGGTTGGTG ACGTCGAGTT CGAGTCGGCC TCCCAGGTCG CTTCCGCCAT AACTCCTGTT 
CCCGGCGGTG TCGGTCCCAT GACGGTTGCT ATGCTTTTGC AGAACGTTGT TGACGCCACT 
AGTTTCTACT TCGAGAACCA GAAGAAGAGG GGATCCATTC CTCTCCCTTT AAAGCTGGCG 
GAGCCCGTTC CTTCCGATAT CGATATTTCC AGGGCCCAGG TTCCCAAGCC CATCACCCAA 
ATCGCCACCG AGATCGGTCT TGCGCCCTCC GAGCTCGAGC CCTACGGTGC TTACAAGGCC 
AAGGTTGACC TCAGCATCCT CAAGCGCCTT GAGCACCGCC GTAACGGTCG CTATGTCGTT 
GTCACTGGTA TCACCCCCAC TCCTCTCGGT GAGGGCAAGT CCACCACCAC TATGGGCCTG 
GCTCAGGCTC TTGGTGCTCA CGTTGGTCGT TTGACTTTCG CCAACGTCCG CCAGCCCAGC 
CAGGGTCCTA CTTTCGGCAT CAAGGGTGGT GCTGCTGGTG GTGGTTACAG TCAGGTCATC 
CCCATGGATG AGTTCAACCT TCACTTGACT GGTGATATCC ATGCCATCAC CGCCGCCAAC 
AACCTTCTCG CTGCCGCTAT CGATACCCGC ATGTTCCATG AGAACACCCA GAAGGACGGT 
CCTCTTTACA AACGTCTAGT CCCTGCCAAA AACGGAAAGA GGGAGTTCCC TCCCATCATG 
TTCCGCCGCT TGAAGAAGCT TGGCATTGAG AAGACGAACC CCGATGATCT CACCGAGGAG 
GAGATTAGCC GCTTTGCTCG TCTCGATATT GACCCCGAGA CCATTACCTG GAGACGTGTT 
CTGGATGTAA ACGACCGTCA TCTGCGCGGT ATCGTTATCG GCAATGCTCC CACCGAGAAG 
GGCCACAAGC GTGACACTGG CTTCGACATT TCTGTTGCCA GTGAATGCAT GGCTATTCTG 
GCCCTCAGTA CTAGTCTTGC TGACATGCGT GAGCGTCTCG GCAGAATGGT TGTTGCCAGC 
TCCAAGAGCG GCGACCCTGT CACTGCTGAC GACCTTGGTG TTGGCGGTGC CCTGACTGTT 
TTGATGAGGG ATGCCATCAA GCCAAACCTC ATGCAGACCT TGGAGGGCAC TCCAGTCTTC 
GTTCACGCCG GTCCTTTCGC CAACATTAGT ATCGGTAACA GCTCGATCCT GGCCGACAAG 
ATGGCCCTCA AGCTTGCCGG TACGGAGCCC GATGAGGATT ACGCTACCAA GTCTGGTTTT 
GTCGTTACTG AAGCCGGTTT CGACTTCACC ATGGGTGGCG AGCGTTTCTT CAACATCAAG 
TGCCGCACCT CTGGCCTCAT GCCTGACGTT GTTGTTGTTG TCGCTACTAT CCGTGCCCTC 
AAAGTTCATG GTGGTGGTCC CCCGATCTCG CCCGGTGCGC CGCTCAACCC TGTGTACAAG 
GAGGAGAATG TTGAGATTCT GCGCGCTGGT TGCGTCAACC TCGCCAAGCA CATCCAGAAT 
GCCCGTTCGT ATGGTGTCCC TGTCGTCGTT GCCATCAACA AGTTCTCGAC CGATACCCAG 
GCCGAGATTG ACGTTGTTCG CGAGGAGGCC ATCAGGGCCG GTGCCGAGGA CGCCGTGCTC 
GCCAACCATT GGGCCGAGGG TGGTAAGGGT GCTGTTGACC TCGCTCAGGC CGTCATTTCT 
GCTAGCGAGA AGTCTACCCC TGAGACTCAG AAGCTTCTAT ATGAGGTTGA GGGCAACACC 
GTCCAGGAGC GTATCGAGAA GATCGCTTGT GAGATGTACG GTGCCGCCAG CGTCGAGTTC 
AGCGAGCTCG CCCAGAAGAA GGTCGATACC TATACTCGCC AGGGATTCGG CAACCTGCCC 
ATCTGCATCG CCAAGACCCA ATATTCACTC AGCCACGACC CCGAGCTTAA GGGTGCCCCC 
ACCGGCTTCA CCGTCCCCAT CAGAGATGTC CGTATGGCCG CGGGCGCCGG GTATCTCTAC 
GCTTTGGCTG CCGATATCCA GACTATTCCT GGTCTGCCTA CGGCTCCTGG TTACTTTAAT 
GTGGACGTGG ATACCGAGAC TGGTGAGATC CAGGGTCTGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394879.1
CDS	160..2964
Translation	MASKIDGTALAKRIRDGLRATIDETKKTNPRFRPCLKIIQATYVRMKLKAAEECGIEAELIKFDENATEAELVAKVVQLNNDSTVHGMLVQLPLPKHISEYAVTSAVADEKDVDGFGTLNIGELAKRGGHPYFIPCTPKGVMALLAEAGVELKGKNAVVLGRSDIVGSPVSYLLKNADATVTVCHSKTADLQQYLKEADVVVAAIGQPNFVKGEWLKKGAVVIDVGTNYVPDDTKKSGVRLVGDVEFESASQVASAITPVPGGVGPMTVAMLLQNVVDATSFYFENQKKRGSIPLPLKLAEPVPSDIDISRAQVPKPITQIATEIGLAPSELEPYGAYKAKVDLSILKRLEHRRNGRYVVVTGITPTPLGEGKSTTTMGLAQALGAHVGRLTFANVRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRMFHENTQKDGPLYKRLVPAKNGKREFPPIMFRRLKKLGIEKTNPDDLTEEEISRFARLDIDPETITWRRVLDVNDRHLRGIVIGNAPTEKGHKRDTGFDISVASECMAILALSTSLADMRERLGRMVVASSKSGDPVTADDLGVGGALTVLMRDAIKPNLMQTLEGTPVFVHAGPFANISIGNSSILADKMALKLAGTEPDEDYATKSGFVVTEAGFDFTMGGERFFNIKCRTSGLMPDVVVVVATIRALKVHGGGPPISPGAPLNPVYKEENVEILRAGCVNLAKHIQNARSYGVPVVVAINKFSTDTQAEIDVVREEAIRAGAEDAVLANHWAEGGKGAVDLAQAVISASEKSTPETQKLLYEVEGNTVQERIEKIACEMYGAASVEFSELAQKKVDTYTRQGFGNLPICIAKTQYSLSHDPELKGAPTGFTVPIRDVRMAAGAGYLYALAADIQTIPGLPTAPGYFNVDVDTETGEIQGLF

Target ORF information:

RefSeq Version	XM_011396577.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A C-1-tetrahydrofolate synthase, variant (NCU03877), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396577.1

ORF Insert Sequence:

ATGGCCTCCA AGATCGACGG CACCGCGCTG GCCAAGCGCA TCCGCGACGG TCTCCGCGCT 
ACCATCGACG AGACCAAGAA GACCAACCCG CGCTTCCGCC CCTGCCTCAA GATCATCCAA 
GCTACCTATG TGCGCATGAA GTTGAAGGCT GCTGAGGAGT GCGGTATCGA GGCCGAGCTG 
ATCAAGTTCG ACGAGAATGC CACCGAGGCC GAGCTTGTTG CCAAGGTGGT GCAGCTCAAC 
AACGACTCGA CCGTCCATGG CATGCTCGTC CAGTTGCCCC TCCCCAAGCA CATTTCCGAG 
TATGCTGTCA CATCGGCCGT TGCCGACGAG AAGGATGTCG ACGGTTTCGG CACCCTCAAC 
ATCGGCGAGC TTGCCAAGCG CGGCGGCCAC CCTTATTTCA TCCCCTGCAC CCCCAAGGGT 
GTCATGGCTC TTCTTGCCGA GGCTGGTGTT GAGCTCAAGG GAAAGAACGC TGTTGTCCTC 
GGTCGTAGCG ATATCGTGGG CAGCCCTGTC AGCTACCTCC TCAAGAACGC CGACGCCACC 
GTCACCGTCT GCCACTCCAA GACCGCCGAT CTCCAGCAGT ATCTCAAGGA GGCCGACGTT 
GTTGTTGCTG CCATTGGCCA GCCTAACTTT GTCAAGGGCG AGTGGCTCAA GAAGGGTGCC 
GTTGTCATTG ATGTCGGTAC CAATTACGTT CCCGACGATA CAAAGAAGTC TGGCGTCCGC 
CTGGTTGGTG ACGTCGAGTT CGAGTCGGCC TCCCAGGTCG CTTCCGCCAT AACTCCTGTT 
CCCGGCGGTG TCGGTCCCAT GACGGTTGCT ATGCTTTTGC AGAACGTTGT TGACGCCACT 
AGTTTCTACT TCGAGAACCA GAAGAAGAGG GGATCCATTC CTCTCCCTTT AAAGCTGGCG 
GAGCCCGTTC CTTCCGATAT CGATATTTCC AGGGCCCAGG TTCCCAAGCC CATCACCCAA 
ATCGCCACCG AGATCGGTCT TGCGCCCTCC GAGCTCGAGC CCTACGGTGC TTACAAGGCC 
AAGGTTGACC TCAGCATCCT CAAGCGCCTT GAGCACCGCC GTAACGGTCG CTATGTCGTT 
GTCACTGGTA TCACCCCCAC TCCTCTCGGT GAGGGCAAGT CCACCACCAC TATGGGCCTG 
GCTCAGGCTC TTGGTGCTCA CGTTGGTCGT TTGACTTTCG CCAACGTCCG CCAGCCCAGC 
CAGGGTCCTA CTTTCGGCAT CAAGGGTGGT GCTGCTGGTG GTGGTTACAG TCAGGTCATC 
CCCATGGATG AGTTCAACCT TCACTTGACT GGTGATATCC ATGCCATCAC CGCCGCCAAC 
AACCTTCTCG CTGCCGCTAT CGATACCCGC ATGTTCCATG AGAACACCCA GAAGGACGGT 
CCTCTTTACA AACGTCTAGT CCCTGCCAAA AACGGAAAGA GGGAGTTCCC TCCCATCATG 
TTCCGCCGCT TGAAGAAGCT TGGCATTGAG AAGACGAACC CCGATGATCT CACCGAGGAG 
GAGATTAGCC GCTTTGCTCG TCTCGATATT GACCCCGAGA CCATTACCTG GAGACGTGTT 
CTGGATGTAA ACGACCGTCA TCTGCGCGGT ATCGTTATCG GCAATGCTCC CACCGAGAAG 
GGCCACAAGC GTGACACTGG CTTCGACATT TCTGTTGCCA GTGAATGCAT GGCTATTCTG 
GCCCTCAGTA CTAGTCTTGC TGACATGCGT GAGCGTCTCG GCAGAATGGT TGTTGCCAGC 
TCCAAGAGCG GCGACCCTGT CACTGCTGAC GACCTTGGTG TTGGCGGTGC CCTGACTGTT 
TTGATGAGGG ATGCCATCAA GCCAAACCTC ATGCAGACCT TGGAGGGCAC TCCAGTCTTC 
GTTCACGCCG GTCCTTTCGC CAACATTAGT ATCGGTAACA GCTCGATCCT GGCCGACAAG 
ATGGCCCTCA AGCTTGCCGG TACGGAGCCC GATGAGGATT ACGCTACCAA GTCTGGTTTT 
GTCGTTACTG AAGCCGGTTT CGACTTCACC ATGGGTGGCG AGCGTTTCTT CAACATCAAG 
TGCCGCACCT CTGGCCTCAT GCCTGACGTT GTTGTTGTTG TCGCTACTAT CCGTGCCCTC 
AAAGTTCATG GTGGTGGTCC CCCGATCTCG CCCGGTGCGC CGCTCAACCC TGTGTACAAG 
GAGGAGAATG TTGAGATTCT GCGCGCTGGT TGCGTCAACC TCGCCAAGCA CATCCAGAAT 
GCCCGTTCGT ATGGTGTCCC TGTCGTCGTT GCCATCAACA AGTTCTCGAC CGATACCCAG 
GCCGAGATTG ACGTTGTTCG CGAGGAGGCC ATCAGGGCCG GTGCCGAGGA CGCCGTGCTC 
GCCAACCATT GGGCCGAGGG TGGTAAGGGT GCTGTTGACC TCGCTCAGGC CGTCATTTCT 
GCTAGCGAGA AGTCTACCCC TGAGACTCAG AAGCTTCTAT ATGAGGTTGA GGGCAACACC 
GTCCAGGAGC GTATCGAGAA GATCGCTTGT GAGATGTACG GTGCCGCCAG CGTCGAGTTC 
AGCGAGCTCG CCCAGAAGAA GGTCGATACC TATACTCGCC AGGGATTCGG CAACCTGCCC 
ATCTGCATCG CCAAGACCCA ATATTCACTC AGCCACGACC CCGAGCTTAA GGGTGCCCCC 
ACCGGCTTCA CCGTCCCCAT CAGAGATGTC CGTATGGCCG CGGGCGCCGG GTATCTCTAC 
GCTTTGGCTG CCGATATCCA GACTATTCCT GGTCTGCCTA CGGCTCCTGG TTACTTTAAT 
GTGGACGTGG ATACCGAGAC TGGTGAGATC CAGGGTCTGT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03877 ( XM_011396576.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394878.1 Neurospora crassa OR74A C-1-tetrahydrofolate synthase (NCU03877), mRNA.

Price & Availability↑

CloneID	ONf10093
Clone ID Related Accession (Same CDS sequence)	XM_011396576.1
Accession Version	XM_011396576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	C-1-tetrahydrofolate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

ATGGCCATCT CCAGCTTCAT CCAGAGGGTG TCTTCCACGT CAAGGAAGTC GCGTCGAGCC 
GGAAGCTGTC TCACATCCCT GTCCGCACAC GGGTCGCTGA ACACACGGCG GCGCATTCAA 
GTCGAGCATA CGGGACAAGA CGTGCTCAAA AGATGGACAT TGTCGTGGGG CACGGAATCC 
GACTCTCGGA GCTCTACCTC GGCTTTCGTC GCCTCTGCAG CTGGTACCAC TGACTTCTCC 
GCCGCCCCGC GATCCACCTG CAGCCGCCCT AAGAAATCCC GTTATTGGCG CCTATCTCCA 
GTGACGGGGC TTACGGGGTC GATTCTGGTG TCACCGTCAT GCGCCGGGGC AGCAGCTGCT 
AGCGATTTTG GAGCTAATGC GCTGCTCTCC CCCTCATCTT CAACTCTGCC TCTGCCTGAC 
ACTTTACCCT TTGCACCATC TTCCTCCTCC CGCTTCTCTC ACATTCAGGG TCCTGTCTCT 
CGATACGCTG CTGCTTTTGG CCATCCGCGC AGCTCCATCA GGACATATTC CAGTTTAGCT 
TCTCCCAGAG CCGCAACAAG GGAGCCTGAA GCAGCAATGG CCTCCAAGAT CGACGGCACC 
GCGCTGGCCA AGCGCATCCG CGACGGTCTC CGCGCTACCA TCGACGAGAC CAAGAAGACC 
AACCCGCGCT TCCGCCCCTG CCTCAAGATC ATCCAAGTTG GTGATCGATC CGATTCCTCT 
ACCTATGTGC GCATGAAGTT GAAGGCTGCT GAGGAGTGCG GTATCGAGGC CGAGCTGATC 
AAGTTCGACG AGAATGCCAC CGAGGCCGAG CTTGTTGCCA AGGTGGTGCA GCTCAACAAC 
GACTCGACCG TCCATGGCAT GCTCGTCCAG TTGCCCCTCC CCAAGCACAT TTCCGAGTAT 
GCTGTCACAT CGGCCGTTGC CGACGAGAAG GATGTCGACG GTTTCGGCAC CCTCAACATC 
GGCGAGCTTG CCAAGCGCGG CGGCCACCCT TATTTCATCC CCTGCACCCC CAAGGGTGTC 
ATGGCTCTTC TTGCCGAGGC TGGTGTTGAG CTCAAGGGAA AGAACGCTGT TGTCCTCGGT 
CGTAGCGATA TCGTGGGCAG CCCTGTCAGC TACCTCCTCA AGAACGCCGA CGCCACCGTC 
ACCGTCTGCC ACTCCAAGAC CGCCGATCTC CAGCAGTATC TCAAGGAGGC CGACGTTGTT 
GTTGCTGCCA TTGGCCAGCC TAACTTTGTC AAGGGCGAGT GGCTCAAGAA GGGTGCCGTT 
GTCATTGATG TCGGTACCAA TTACGTTCCC GACGATACAA AGAAGTCTGG CGTCCGCCTG 
GTTGGTGACG TCGAGTTCGA GTCGGCCTCC CAGGTCGCTT CCGCCATAAC TCCTGTTCCC 
GGCGGTGTCG GTCCCATGAC GGTTGCTATG CTTTTGCAGA ACGTTGTTGA CGCCACTAGT 
TTCTACTTCG AGAACCAGAA GAAGAGGGGA TCCATTCCTC TCCCTTTAAA GCTGGCGGAG 
CCCGTTCCTT CCGATATCGA TATTTCCAGG GCCCAGGTTC CCAAGCCCAT CACCCAAATC 
GCCACCGAGA TCGGTCTTGC GCCCTCCGAG CTCGAGCCCT ACGGTGCTTA CAAGGCCAAG 
GTTGACCTCA GCATCCTCAA GCGCCTTGAG CACCGCCGTA ACGGTCGCTA TGTCGTTGTC 
ACTGGTATCA CCCCCACTCC TCTCGGTGAG GGCAAGTCCA CCACCACTAT GGGCCTGGCT 
CAGGCTCTTG GTGCTCACGT TGGTCGTTTG ACTTTCGCCA ACGTCCGCCA GCCCAGCCAG 
GGTCCTACTT TCGGCATCAA GGGTGGTGCT GCTGGTGGTG GTTACAGTCA GGTCATCCCC 
ATGGATGAGT TCAACCTTCA CTTGACTGGT GATATCCATG CCATCACCGC CGCCAACAAC 
CTTCTCGCTG CCGCTATCGA TACCCGCATG TTCCATGAGA ACACCCAGAA GGACGGTCCT 
CTTTACAAAC GTCTAGTCCC TGCCAAAAAC GGAAAGAGGG AGTTCCCTCC CATCATGTTC 
CGCCGCTTGA AGAAGCTTGG CATTGAGAAG ACGAACCCCG ATGATCTCAC CGAGGAGGAG 
ATTAGCCGCT TTGCTCGTCT CGATATTGAC CCCGAGACCA TTACCTGGAG ACGTGTTCTG 
GATGTAAACG ACCGTCATCT GCGCGGTATC GTTATCGGCA ATGCTCCCAC CGAGAAGGGC 
CACAAGCGTG ACACTGGCTT CGACATTTCT GTTGCCAGTG AATGCATGGC TATTCTGGCC 
CTCAGTACTA GTCTTGCTGA CATGCGTGAG CGTCTCGGCA GAATGGTTGT TGCCAGCTCC 
AAGAGCGGCG ACCCTGTCAC TGCTGACGAC CTTGGTGTTG GCGGTGCCCT GACTGTTTTG 
ATGAGGGATG CCATCAAGCC AAACCTCATG CAGACCTTGG AGGGCACTCC AGTCTTCGTT 
CACGCCGGTC CTTTCGCCAA CATTAGTATC GGTAACAGCT CGATCCTGGC CGACAAGATG 
GCCCTCAAGC TTGCCGGTAC GGAGCCCGAT GAGGATTACG CTACCAAGTC TGGTTTTGTC 
GTTACTGAAG CCGGTTTCGA CTTCACCATG GGTGGCGAGC GTTTCTTCAA CATCAAGTGC 
CGCACCTCTG GCCTCATGCC TGACGTTGTT GTTGTTGTCG CTACTATCCG TGCCCTCAAA 
GTTCATGGTG GTGGTCCCCC GATCTCGCCC GGTGCGCCGC TCAACCCTGT GTACAAGGAG 
GAGAATGTTG AGATTCTGCG CGCTGGTTGC GTCAACCTCG CCAAGCACAT CCAGAATGCC 
CGTTCGTATG GTGTCCCTGT CGTCGTTGCC ATCAACAAGT TCTCGACCGA TACCCAGGCC 
GAGATTGACG TTGTTCGCGA GGAGGCCATC AGGGCCGGTG CCGAGGACGC CGTGCTCGCC 
AACCATTGGG CCGAGGGTGG TAAGGGTGCT GTTGACCTCG CTCAGGCCGT CATTTCTGCT 
AGCGAGAAGT CTACCCCTGA GACTCAGAAG CTTCTATATG AGGTTGAGGG CAACACCGTC 
CAGGAGCGTA TCGAGAAGAT CGCTTGTGAG ATGTACGGTG CCGCCAGCGT CGAGTTCAGC 
GAGCTCGCCC AGAAGAAGGT CGATACCTAT ACTCGCCAGG GATTCGGCAA CCTGCCCATC 
TGCATCGCCA AGACCCAATA TTCACTCAGC CACGACCCCG AGCTTAAGGG TGCCCCCACC 
GGCTTCACCG TCCCCATCAG AGATGTCCGT ATGGCCGCGG GCGCCGGGTA TCTCTACGCT 
TTGGCTGCCG ATATCCAGAC TATTCCTGGT CTGCCTACGG CTCCTGGTTA CTTTAATGTG 
GACGTGGATA CCGAGACTGG TGAGATCCAG GGTCTGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394878.1
CDS	593..3994
Translation	MAISSFIQRVSSTSRKSRRAGSCLTSLSAHGSLNTRRRIQVEHTGQDVLKRWTLSWGTESDSRSSTSAFVASAAGTTDFSAAPRSTCSRPKKSRYWRLSPVTGLTGSILVSPSCAGAAAASDFGANALLSPSSSTLPLPDTLPFAPSSSSRFSHIQGPVSRYAAAFGHPRSSIRTYSSLASPRAATREPEAAMASKIDGTALAKRIRDGLRATIDETKKTNPRFRPCLKIIQVGDRSDSSTYVRMKLKAAEECGIEAELIKFDENATEAELVAKVVQLNNDSTVHGMLVQLPLPKHISEYAVTSAVADEKDVDGFGTLNIGELAKRGGHPYFIPCTPKGVMALLAEAGVELKGKNAVVLGRSDIVGSPVSYLLKNADATVTVCHSKTADLQQYLKEADVVVAAIGQPNFVKGEWLKKGAVVIDVGTNYVPDDTKKSGVRLVGDVEFESASQVASAITPVPGGVGPMTVAMLLQNVVDATSFYFENQKKRGSIPLPLKLAEPVPSDIDISRAQVPKPITQIATEIGLAPSELEPYGAYKAKVDLSILKRLEHRRNGRYVVVTGITPTPLGEGKSTTTMGLAQALGAHVGRLTFANVRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRMFHENTQKDGPLYKRLVPAKNGKREFPPIMFRRLKKLGIEKTNPDDLTEEEISRFARLDIDPETITWRRVLDVNDRHLRGIVIGNAPTEKGHKRDTGFDISVASECMAILALSTSLADMRERLGRMVVASSKSGDPVTADDLGVGGALTVLMRDAIKPNLMQTLEGTPVFVHAGPFANISIGNSSILADKMALKLAGTEPDEDYATKSGFVVTEAGFDFTMGGERFFNIKCRTSGLMPDVVVVVATIRALKVHGGGPPISPGAPLNPVYKEENVEILRAGCVNLAKHIQNARSYGVPVVVAINKFSTDTQAEIDVVREEAIRAGAEDAVLANHWAEGGKGAVDLAQAVISASEKSTPETQKLLYEVEGNTVQERIEKIACEMYGAASVEFSELAQKKVDTYTRQGFGNLPICIAKTQYSLSHDPELKGAPTGFTVPIRDVRMAAGAGYLYALAADIQTIPGLPTAPGYFNVDVDTETGEIQGLF

Target ORF information:

RefSeq Version	XM_011396576.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A C-1-tetrahydrofolate synthase (NCU03877), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396576.1

ORF Insert Sequence:

ATGGCCATCT CCAGCTTCAT CCAGAGGGTG TCTTCCACGT CAAGGAAGTC GCGTCGAGCC 
GGAAGCTGTC TCACATCCCT GTCCGCACAC GGGTCGCTGA ACACACGGCG GCGCATTCAA 
GTCGAGCATA CGGGACAAGA CGTGCTCAAA AGATGGACAT TGTCGTGGGG CACGGAATCC 
GACTCTCGGA GCTCTACCTC GGCTTTCGTC GCCTCTGCAG CTGGTACCAC TGACTTCTCC 
GCCGCCCCGC GATCCACCTG CAGCCGCCCT AAGAAATCCC GTTATTGGCG CCTATCTCCA 
GTGACGGGGC TTACGGGGTC GATTCTGGTG TCACCGTCAT GCGCCGGGGC AGCAGCTGCT 
AGCGATTTTG GAGCTAATGC GCTGCTCTCC CCCTCATCTT CAACTCTGCC TCTGCCTGAC 
ACTTTACCCT TTGCACCATC TTCCTCCTCC CGCTTCTCTC ACATTCAGGG TCCTGTCTCT 
CGATACGCTG CTGCTTTTGG CCATCCGCGC AGCTCCATCA GGACATATTC CAGTTTAGCT 
TCTCCCAGAG CCGCAACAAG GGAGCCTGAA GCAGCAATGG CCTCCAAGAT CGACGGCACC 
GCGCTGGCCA AGCGCATCCG CGACGGTCTC CGCGCTACCA TCGACGAGAC CAAGAAGACC 
AACCCGCGCT TCCGCCCCTG CCTCAAGATC ATCCAAGTTG GTGATCGATC CGATTCCTCT 
ACCTATGTGC GCATGAAGTT GAAGGCTGCT GAGGAGTGCG GTATCGAGGC CGAGCTGATC 
AAGTTCGACG AGAATGCCAC CGAGGCCGAG CTTGTTGCCA AGGTGGTGCA GCTCAACAAC 
GACTCGACCG TCCATGGCAT GCTCGTCCAG TTGCCCCTCC CCAAGCACAT TTCCGAGTAT 
GCTGTCACAT CGGCCGTTGC CGACGAGAAG GATGTCGACG GTTTCGGCAC CCTCAACATC 
GGCGAGCTTG CCAAGCGCGG CGGCCACCCT TATTTCATCC CCTGCACCCC CAAGGGTGTC 
ATGGCTCTTC TTGCCGAGGC TGGTGTTGAG CTCAAGGGAA AGAACGCTGT TGTCCTCGGT 
CGTAGCGATA TCGTGGGCAG CCCTGTCAGC TACCTCCTCA AGAACGCCGA CGCCACCGTC 
ACCGTCTGCC ACTCCAAGAC CGCCGATCTC CAGCAGTATC TCAAGGAGGC CGACGTTGTT 
GTTGCTGCCA TTGGCCAGCC TAACTTTGTC AAGGGCGAGT GGCTCAAGAA GGGTGCCGTT 
GTCATTGATG TCGGTACCAA TTACGTTCCC GACGATACAA AGAAGTCTGG CGTCCGCCTG 
GTTGGTGACG TCGAGTTCGA GTCGGCCTCC CAGGTCGCTT CCGCCATAAC TCCTGTTCCC 
GGCGGTGTCG GTCCCATGAC GGTTGCTATG CTTTTGCAGA ACGTTGTTGA CGCCACTAGT 
TTCTACTTCG AGAACCAGAA GAAGAGGGGA TCCATTCCTC TCCCTTTAAA GCTGGCGGAG 
CCCGTTCCTT CCGATATCGA TATTTCCAGG GCCCAGGTTC CCAAGCCCAT CACCCAAATC 
GCCACCGAGA TCGGTCTTGC GCCCTCCGAG CTCGAGCCCT ACGGTGCTTA CAAGGCCAAG 
GTTGACCTCA GCATCCTCAA GCGCCTTGAG CACCGCCGTA ACGGTCGCTA TGTCGTTGTC 
ACTGGTATCA CCCCCACTCC TCTCGGTGAG GGCAAGTCCA CCACCACTAT GGGCCTGGCT 
CAGGCTCTTG GTGCTCACGT TGGTCGTTTG ACTTTCGCCA ACGTCCGCCA GCCCAGCCAG 
GGTCCTACTT TCGGCATCAA GGGTGGTGCT GCTGGTGGTG GTTACAGTCA GGTCATCCCC 
ATGGATGAGT TCAACCTTCA CTTGACTGGT GATATCCATG CCATCACCGC CGCCAACAAC 
CTTCTCGCTG CCGCTATCGA TACCCGCATG TTCCATGAGA ACACCCAGAA GGACGGTCCT 
CTTTACAAAC GTCTAGTCCC TGCCAAAAAC GGAAAGAGGG AGTTCCCTCC CATCATGTTC 
CGCCGCTTGA AGAAGCTTGG CATTGAGAAG ACGAACCCCG ATGATCTCAC CGAGGAGGAG 
ATTAGCCGCT TTGCTCGTCT CGATATTGAC CCCGAGACCA TTACCTGGAG ACGTGTTCTG 
GATGTAAACG ACCGTCATCT GCGCGGTATC GTTATCGGCA ATGCTCCCAC CGAGAAGGGC 
CACAAGCGTG ACACTGGCTT CGACATTTCT GTTGCCAGTG AATGCATGGC TATTCTGGCC 
CTCAGTACTA GTCTTGCTGA CATGCGTGAG CGTCTCGGCA GAATGGTTGT TGCCAGCTCC 
AAGAGCGGCG ACCCTGTCAC TGCTGACGAC CTTGGTGTTG GCGGTGCCCT GACTGTTTTG 
ATGAGGGATG CCATCAAGCC AAACCTCATG CAGACCTTGG AGGGCACTCC AGTCTTCGTT 
CACGCCGGTC CTTTCGCCAA CATTAGTATC GGTAACAGCT CGATCCTGGC CGACAAGATG 
GCCCTCAAGC TTGCCGGTAC GGAGCCCGAT GAGGATTACG CTACCAAGTC TGGTTTTGTC 
GTTACTGAAG CCGGTTTCGA CTTCACCATG GGTGGCGAGC GTTTCTTCAA CATCAAGTGC 
CGCACCTCTG GCCTCATGCC TGACGTTGTT GTTGTTGTCG CTACTATCCG TGCCCTCAAA 
GTTCATGGTG GTGGTCCCCC GATCTCGCCC GGTGCGCCGC TCAACCCTGT GTACAAGGAG 
GAGAATGTTG AGATTCTGCG CGCTGGTTGC GTCAACCTCG CCAAGCACAT CCAGAATGCC 
CGTTCGTATG GTGTCCCTGT CGTCGTTGCC ATCAACAAGT TCTCGACCGA TACCCAGGCC 
GAGATTGACG TTGTTCGCGA GGAGGCCATC AGGGCCGGTG CCGAGGACGC CGTGCTCGCC 
AACCATTGGG CCGAGGGTGG TAAGGGTGCT GTTGACCTCG CTCAGGCCGT CATTTCTGCT 
AGCGAGAAGT CTACCCCTGA GACTCAGAAG CTTCTATATG AGGTTGAGGG CAACACCGTC 
CAGGAGCGTA TCGAGAAGAT CGCTTGTGAG ATGTACGGTG CCGCCAGCGT CGAGTTCAGC 
GAGCTCGCCC AGAAGAAGGT CGATACCTAT ACTCGCCAGG GATTCGGCAA CCTGCCCATC 
TGCATCGCCA AGACCCAATA TTCACTCAGC CACGACCCCG AGCTTAAGGG TGCCCCCACC 
GGCTTCACCG TCCCCATCAG AGATGTCCGT ATGGCCGCGG GCGCCGGGTA TCTCTACGCT 
TTGGCTGCCG ATATCCAGAC TATTCCTGGT CTGCCTACGG CTCCTGGTTA CTTTAATGTG 
GACGTGGATA CCGAGACTGG TGAGATCCAG GGTCTGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03877 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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