MTHFD1 cDNA ORF clone, Canis lupus familiaris(dog)

The following MTHFD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe10509 XM_537476.6
Latest version!
Canis lupus familiaris methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe10510 XM_005623492.3
Latest version!
Canis lupus familiaris methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OCe10509 XM_537476.5 Canis lupus familiaris methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe10510 XM_005623492.2 Canis lupus familiaris methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe10509
    Clone ID Related Accession (Same CDS sequence) XM_537476.6 , XM_537476.5
    Accession Version XM_537476.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2808bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537476.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCACCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
    AAGAATGAAG TCACTCAGAT GAAGGAACAA GTTCCTGGTT TTATACCAGG CCTGGCAATT
    TTACAGGTTG GCGACAGAGA TGATTCTAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT
    GAAGAGATTG GGATCAGAGC CACGCACATT AAGTTACCAA AAACAGCCAC AGAATCTGAG
    GTATTAAAGT ATGTTACATC ATTGAATGAA GACCTCACTG TCCATGGTTT CATAGTGCAG
    CTGCCTTTAG ATTCAGAGAA CCCCATTAAC ACTGAAGCAG TGATCAATGC TATTGTACCC
    GAAAAGGATG TGGATGGGTT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGATCTG
    AAAGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTAATCAA AGAGACAGGG
    GTACAGATCG CTGGAAGGCA CGCTGTAGTA GTTGGGCGCA GTAAGATAGT TGGTGCCCCG
    ATGCATGACC TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC
    AATCTAAGCG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCAGAA
    ATGGTAAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGCGG GATCAATTAT
    ATTCCAGATG ATACAAAACC AAATGGGAGA AAAGTGGTGG GTGATGTGGC ATACAATGAG
    GCCAAGGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGCG GCGTGGGGCC CATGACGGTG
    GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCGAAGCGTT TCCTGGAGAA ATTTAAGCCA
    GGAAAGTGGA TGATTCAATA TAACAACCTT AACCTCAAGA CTCCTGTTCC AAGTGACATT
    GATATATCAC GGTCTTGTAA GCCAAAGCCC ATTGGCAGCC TGGCGCGGGA AATTGGTCTG
    CTCTCTGAAG AGGTGGAATT ATATGGTGAT ACAAAGGCCA AGGTTCTGCT ATCAGCACTG
    GAACGCCTGA AGCACCAGCC TGATGGAAAA TACGTGGTGG TGACTGGAAT CACCCCAACG
    CCCCTGGGAG AAGGGAAAAG CACAACCACC ATTGGGCTGG TGCAGGCCCT TGGCGCCCAT
    CTTCATCAGA ACGTCTTTGC ATGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA
    AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CCATGGAAGA GTTTAATCTC
    CACCTCACTG GTGACATCCA TGCCATCACT GCAGCGAATA ACCTTGTCGC CGCGGCCATC
    GATGCCCGGA TGTTCCACGA ACTGACGCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA
    CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATCCAAA TCCGAAGATT ACAGAGACTG
    GGTATTGAAA AAACTGACCC TACCACACTG ACTGATGACG AGATAAACAG ATTTGCAAGA
    TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTAT TGGATACCAA CGATAGATTC
    CTAAGGAAGA TCACAATCGG ACAGGCTCCA ACAGAAAAAG GGCACACACG GACGGCCCAG
    TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTGCTGG CTCTCACCAG TTCACTAGAA
    GACATGAGAG AGAGACTGAG CAAAATGGTG GTGGCATCCA GCAAGAAAGG GGAGCCTATC
    AGTACCGAAG ATCTGGGGGT GAGTGGTGCA TTGACAGTGC TTATGAAGGA TGCAATCAAG
    CCTAATCTCA TGCAGACATT AGAGGGCACT CCAGTTTTCG TTCATGCTGG ACCATTTGCC
    AACATCGCAC ATGGGAATTC CTCTATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC
    CCTGAAGGGT TCGTGGTGAC TGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT
    TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCTCATG TAGTGGTGCT TGTTGCCACT
    GTCAGGGCTC TTAAAATGCA CGGAGGCGGC CCCATGGTTA CTGCTGGACT GCCTCTTCCC
    GAGGCTTACA TAGTGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTAAAGAAA
    CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCTGTGGTCG TGGCCGTGAA TGCATTCAAG
    ACAGATACGG AGGCCGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT
    GATGCTGTGA AATGCACGCA CTGGGCAGAA GGGGGTATGG GTGCCTTAGC CCTAGCTCAG
    GCTGTCCAGA GAGCAGCTCA GGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC
    CCAGTTGAGG ATAAAATCAG GATCATCGCA CAGAAAATCT ATGGGGCTGA CGACATAGAA
    TTGCTTCCTG AAGCTCAACA CAAAGCTGAA GTCTACACGA AACAGGGCTT TGGGAACCTG
    CCTATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCATA ACCCAGAGCA AAAGGGTGTG
    CCCACAGGCT TTGTCTTGCC CATTCGTGAC ATCCGTGCCA GCGTCGGGGC CGGTTTTCTG
    TACCCCTTGG TAGGGACGAT GAGCACAATG CCTGGACTCC CCACTCGGCC CTGTTTCTAT
    GATATTGATT TGGACCCTGA AACTGAACAA GTGAATGGGT TGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537476.2
    CDS319..3126
    Translation

    Target ORF information:

    RefSeq Version XM_537476.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537476.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCACCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
    AAGAATGAAG TCACTCAGAT GAAGGAACAA GTTCCTGGTT TTATACCAGG CCTGGCAATT
    TTACAGGTTG GCGACAGAGA TGATTCTAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT
    GAAGAGATTG GGATCAGAGC CACGCACATT AAGTTACCAA AAACAGCCAC AGAATCTGAG
    GTATTAAAGT ATGTTACATC ATTGAATGAA GACCTCACTG TCCATGGTTT CATAGTGCAG
    CTGCCTTTAG ATTCAGAGAA CCCCATTAAC ACTGAAGCAG TGATCAATGC TATTGTACCC
    GAAAAGGATG TGGATGGGTT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGATCTG
    AAAGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTAATCAA AGAGACAGGG
    GTACAGATCG CTGGAAGGCA CGCTGTAGTA GTTGGGCGCA GTAAGATAGT TGGTGCCCCG
    ATGCATGACC TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC
    AATCTAAGCG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCAGAA
    ATGGTAAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGCGG GATCAATTAT
    ATTCCAGATG ATACAAAACC AAATGGGAGA AAAGTGGTGG GTGATGTGGC ATACAATGAG
    GCCAAGGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGCG GCGTGGGGCC CATGACGGTG
    GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCGAAGCGTT TCCTGGAGAA ATTTAAGCCA
    GGAAAGTGGA TGATTCAATA TAACAACCTT AACCTCAAGA CTCCTGTTCC AAGTGACATT
    GATATATCAC GGTCTTGTAA GCCAAAGCCC ATTGGCAGCC TGGCGCGGGA AATTGGTCTG
    CTCTCTGAAG AGGTGGAATT ATATGGTGAT ACAAAGGCCA AGGTTCTGCT ATCAGCACTG
    GAACGCCTGA AGCACCAGCC TGATGGAAAA TACGTGGTGG TGACTGGAAT CACCCCAACG
    CCCCTGGGAG AAGGGAAAAG CACAACCACC ATTGGGCTGG TGCAGGCCCT TGGCGCCCAT
    CTTCATCAGA ACGTCTTTGC ATGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA
    AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CCATGGAAGA GTTTAATCTC
    CACCTCACTG GTGACATCCA TGCCATCACT GCAGCGAATA ACCTTGTCGC CGCGGCCATC
    GATGCCCGGA TGTTCCACGA ACTGACGCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA
    CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATCCAAA TCCGAAGATT ACAGAGACTG
    GGTATTGAAA AAACTGACCC TACCACACTG ACTGATGACG AGATAAACAG ATTTGCAAGA
    TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTAT TGGATACCAA CGATAGATTC
    CTAAGGAAGA TCACAATCGG ACAGGCTCCA ACAGAAAAAG GGCACACACG GACGGCCCAG
    TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTGCTGG CTCTCACCAG TTCACTAGAA
    GACATGAGAG AGAGACTGAG CAAAATGGTG GTGGCATCCA GCAAGAAAGG GGAGCCTATC
    AGTACCGAAG ATCTGGGGGT GAGTGGTGCA TTGACAGTGC TTATGAAGGA TGCAATCAAG
    CCTAATCTCA TGCAGACATT AGAGGGCACT CCAGTTTTCG TTCATGCTGG ACCATTTGCC
    AACATCGCAC ATGGGAATTC CTCTATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC
    CCTGAAGGGT TCGTGGTGAC TGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT
    TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCTCATG TAGTGGTGCT TGTTGCCACT
    GTCAGGGCTC TTAAAATGCA CGGAGGCGGC CCCATGGTTA CTGCTGGACT GCCTCTTCCC
    GAGGCTTACA TAGTGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTAAAGAAA
    CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCTGTGGTCG TGGCCGTGAA TGCATTCAAG
    ACAGATACGG AGGCCGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT
    GATGCTGTGA AATGCACGCA CTGGGCAGAA GGGGGTATGG GTGCCTTAGC CCTAGCTCAG
    GCTGTCCAGA GAGCAGCTCA GGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC
    CCAGTTGAGG ATAAAATCAG GATCATCGCA CAGAAAATCT ATGGGGCTGA CGACATAGAA
    TTGCTTCCTG AAGCTCAACA CAAAGCTGAA GTCTACACGA AACAGGGCTT TGGGAACCTG
    CCTATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCATA ACCCAGAGCA AAAGGGTGTG
    CCCACAGGCT TTGTCTTGCC CATTCGTGAC ATCCGTGCCA GCGTCGGGGC CGGTTTTCTG
    TACCCCTTGG TAGGGACGAT GAGCACAATG CCTGGACTCC CCACTCGGCC CTGTTTCTAT
    GATATTGATT TGGACCCTGA AACTGAACAA GTGAATGGGT TGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10509
    Clone ID Related Accession (Same CDS sequence) XM_537476.6 , XM_537476.5
    Accession Version XM_537476.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2808bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537476.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCACCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
    AAGAATGAAG TCACTCAGAT GAAGGAACAA GTTCCTGGTT TTATACCAGG CCTGGCAATT
    TTACAGGTTG GCGACAGAGA TGATTCTAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT
    GAAGAGATTG GGATCAGAGC CACGCACATT AAGTTACCAA AAACAGCCAC AGAATCTGAG
    GTATTAAAGT ATGTTACATC ATTGAATGAA GACCTCACTG TCCATGGTTT CATAGTGCAG
    CTGCCTTTAG ATTCAGAGAA CCCCATTAAC ACTGAAGCAG TGATCAATGC TATTGTACCC
    GAAAAGGATG TGGATGGGTT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGATCTG
    AAAGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTAATCAA AGAGACAGGG
    GTACAGATCG CTGGAAGGCA CGCTGTAGTA GTTGGGCGCA GTAAGATAGT TGGTGCCCCG
    ATGCATGACC TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC
    AATCTAAGCG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCAGAA
    ATGGTAAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGCGG GATCAATTAT
    ATTCCAGATG ATACAAAACC AAATGGGAGA AAAGTGGTGG GTGATGTGGC ATACAATGAG
    GCCAAGGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGCG GCGTGGGGCC CATGACGGTG
    GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCGAAGCGTT TCCTGGAGAA ATTTAAGCCA
    GGAAAGTGGA TGATTCAATA TAACAACCTT AACCTCAAGA CTCCTGTTCC AAGTGACATT
    GATATATCAC GGTCTTGTAA GCCAAAGCCC ATTGGCAGCC TGGCGCGGGA AATTGGTCTG
    CTCTCTGAAG AGGTGGAATT ATATGGTGAT ACAAAGGCCA AGGTTCTGCT ATCAGCACTG
    GAACGCCTGA AGCACCAGCC TGATGGAAAA TACGTGGTGG TGACTGGAAT CACCCCAACG
    CCCCTGGGAG AAGGGAAAAG CACAACCACC ATTGGGCTGG TGCAGGCCCT TGGCGCCCAT
    CTTCATCAGA ACGTCTTTGC ATGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA
    AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CCATGGAAGA GTTTAATCTC
    CACCTCACTG GTGACATCCA TGCCATCACT GCAGCGAATA ACCTTGTCGC CGCGGCCATC
    GATGCCCGGA TGTTCCACGA ACTGACGCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA
    CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATCCAAA TCCGAAGATT ACAGAGACTG
    GGTATTGAAA AAACTGACCC TACCACACTG ACTGATGACG AGATAAACAG ATTTGCAAGA
    TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTAT TGGATACCAA CGATAGATTC
    CTAAGGAAGA TCACAATCGG ACAGGCTCCA ACAGAAAAAG GGCACACACG GACGGCCCAG
    TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTGCTGG CTCTCACCAG TTCACTAGAA
    GACATGAGAG AGAGACTGAG CAAAATGGTG GTGGCATCCA GCAAGAAAGG GGAGCCTATC
    AGTACCGAAG ATCTGGGGGT GAGTGGTGCA TTGACAGTGC TTATGAAGGA TGCAATCAAG
    CCTAATCTCA TGCAGACATT AGAGGGCACT CCAGTTTTCG TTCATGCTGG ACCATTTGCC
    AACATCGCAC ATGGGAATTC CTCTATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC
    CCTGAAGGGT TCGTGGTGAC TGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT
    TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCTCATG TAGTGGTGCT TGTTGCCACT
    GTCAGGGCTC TTAAAATGCA CGGAGGCGGC CCCATGGTTA CTGCTGGACT GCCTCTTCCC
    GAGGCTTACA TAGTGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTAAAGAAA
    CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCTGTGGTCG TGGCCGTGAA TGCATTCAAG
    ACAGATACGG AGGCCGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT
    GATGCTGTGA AATGCACGCA CTGGGCAGAA GGGGGTATGG GTGCCTTAGC CCTAGCTCAG
    GCTGTCCAGA GAGCAGCTCA GGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC
    CCAGTTGAGG ATAAAATCAG GATCATCGCA CAGAAAATCT ATGGGGCTGA CGACATAGAA
    TTGCTTCCTG AAGCTCAACA CAAAGCTGAA GTCTACACGA AACAGGGCTT TGGGAACCTG
    CCTATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCATA ACCCAGAGCA AAAGGGTGTG
    CCCACAGGCT TTGTCTTGCC CATTCGTGAC ATCCGTGCCA GCGTCGGGGC CGGTTTTCTG
    TACCCCTTGG TAGGGACGAT GAGCACAATG CCTGGACTCC CCACTCGGCC CTGTTTCTAT
    GATATTGATT TGGACCCTGA AACTGAACAA GTGAATGGGT TGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537476.2
    CDS167..2974
    Translation

    Target ORF information:

    RefSeq Version XM_537476.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537476.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCACCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAG GGAGAGACTG 
    AAGAATGAAG TCACTCAGAT GAAGGAACAA GTTCCTGGTT TTATACCAGG CCTGGCAATT
    TTACAGGTTG GCGACAGAGA TGATTCTAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT
    GAAGAGATTG GGATCAGAGC CACGCACATT AAGTTACCAA AAACAGCCAC AGAATCTGAG
    GTATTAAAGT ATGTTACATC ATTGAATGAA GACCTCACTG TCCATGGTTT CATAGTGCAG
    CTGCCTTTAG ATTCAGAGAA CCCCATTAAC ACTGAAGCAG TGATCAATGC TATTGTACCC
    GAAAAGGATG TGGATGGGTT GACTAGCATC AGTGCTGGAA AACTTGCTAG AGGTGATCTG
    AAAGACTGTT TCATCCCTTG TACACCTAAA GGATGCTTGG AACTAATCAA AGAGACAGGG
    GTACAGATCG CTGGAAGGCA CGCTGTAGTA GTTGGGCGCA GTAAGATAGT TGGTGCCCCG
    ATGCATGACC TGCTTCTGTG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC
    AATCTAAGCG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCAGAA
    ATGGTAAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGCGG GATCAATTAT
    ATTCCAGATG ATACAAAACC AAATGGGAGA AAAGTGGTGG GTGATGTGGC ATACAATGAG
    GCCAAGGAGA GGGCGGGCTT CATCACTCCT GTTCCTGGCG GCGTGGGGCC CATGACGGTG
    GCAATGCTAA TGCAGAGCAC AGTGGAGAGT GCGAAGCGTT TCCTGGAGAA ATTTAAGCCA
    GGAAAGTGGA TGATTCAATA TAACAACCTT AACCTCAAGA CTCCTGTTCC AAGTGACATT
    GATATATCAC GGTCTTGTAA GCCAAAGCCC ATTGGCAGCC TGGCGCGGGA AATTGGTCTG
    CTCTCTGAAG AGGTGGAATT ATATGGTGAT ACAAAGGCCA AGGTTCTGCT ATCAGCACTG
    GAACGCCTGA AGCACCAGCC TGATGGAAAA TACGTGGTGG TGACTGGAAT CACCCCAACG
    CCCCTGGGAG AAGGGAAAAG CACAACCACC ATTGGGCTGG TGCAGGCCCT TGGCGCCCAT
    CTTCATCAGA ACGTCTTTGC ATGTGTACGA CAGCCTTCTC AGGGCCCGAC CTTTGGAATA
    AAAGGTGGCG CTGCAGGAGG TGGCTACTCA CAGGTCATTC CCATGGAAGA GTTTAATCTC
    CACCTCACTG GTGACATCCA TGCCATCACT GCAGCGAATA ACCTTGTCGC CGCGGCCATC
    GATGCCCGGA TGTTCCACGA ACTGACGCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTA
    CCATCAGTAA ATGGAGTGAG AAAGTTCTCT GATATCCAAA TCCGAAGATT ACAGAGACTG
    GGTATTGAAA AAACTGACCC TACCACACTG ACTGATGACG AGATAAACAG ATTTGCAAGA
    TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTAT TGGATACCAA CGATAGATTC
    CTAAGGAAGA TCACAATCGG ACAGGCTCCA ACAGAAAAAG GGCACACACG GACGGCCCAG
    TTTGATATCT CTGTGGCCAG TGAAATCATG GCTGTGCTGG CTCTCACCAG TTCACTAGAA
    GACATGAGAG AGAGACTGAG CAAAATGGTG GTGGCATCCA GCAAGAAAGG GGAGCCTATC
    AGTACCGAAG ATCTGGGGGT GAGTGGTGCA TTGACAGTGC TTATGAAGGA TGCAATCAAG
    CCTAATCTCA TGCAGACATT AGAGGGCACT CCAGTTTTCG TTCATGCTGG ACCATTTGCC
    AACATCGCAC ATGGGAATTC CTCTATCATT GCAGACCGGA TTGCACTCAA GCTTGTTGGC
    CCTGAAGGGT TCGTGGTGAC TGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT
    TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCTCATG TAGTGGTGCT TGTTGCCACT
    GTCAGGGCTC TTAAAATGCA CGGAGGCGGC CCCATGGTTA CTGCTGGACT GCCTCTTCCC
    GAGGCTTACA TAGTGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTAAAGAAA
    CAAATTGAAA ATGCCAGAAT GTTTGGAGTT CCTGTGGTCG TGGCCGTGAA TGCATTCAAG
    ACAGATACGG AGGCCGAGCT GGACCTCGTC AGCCGCCTTG CCAAAGAACA TGGGGCTTTT
    GATGCTGTGA AATGCACGCA CTGGGCAGAA GGGGGTATGG GTGCCTTAGC CCTAGCTCAG
    GCTGTCCAGA GAGCAGCTCA GGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC
    CCAGTTGAGG ATAAAATCAG GATCATCGCA CAGAAAATCT ATGGGGCTGA CGACATAGAA
    TTGCTTCCTG AAGCTCAACA CAAAGCTGAA GTCTACACGA AACAGGGCTT TGGGAACCTG
    CCTATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCATA ACCCAGAGCA AAAGGGTGTG
    CCCACAGGCT TTGTCTTGCC CATTCGTGAC ATCCGTGCCA GCGTCGGGGC CGGTTTTCTG
    TACCCCTTGG TAGGGACGAT GAGCACAATG CCTGGACTCC CCACTCGGCC CTGTTTCTAT
    GATATTGATT TGGACCCTGA AACTGAACAA GTGAATGGGT TGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10510
    Clone ID Related Accession (Same CDS sequence) XM_005623492.2 , XM_005623492.3
    Accession Version XM_005623492.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGAAC AAGTTCCTGG TTTTATACCA GGCCTGGCAA TTTTACAGGT TGGCGACAGA 
    GATGATTCTA ATCTTTATAT AAATGTGAAG CTGAAGGCTG CTGAAGAGAT TGGGATCAGA
    GCCACGCACA TTAAGTTACC AAAAACAGCC ACAGAATCTG AGGTATTAAA GTATGTTACA
    TCATTGAATG AAGACCTCAC TGTCCATGGT TTCATAGTGC AGCTGCCTTT AGATTCAGAG
    AACCCCATTA ACACTGAAGC AGTGATCAAT GCTATTGTAC CCGAAAAGGA TGTGGATGGG
    TTGACTAGCA TCAGTGCTGG AAAACTTGCT AGAGGTGATC TGAAAGACTG TTTCATCCCT
    TGTACACCTA AAGGATGCTT GGAACTAATC AAAGAGACAG GGGTACAGAT CGCTGGAAGG
    CACGCTGTAG TAGTTGGGCG CAGTAAGATA GTTGGTGCCC CGATGCATGA CCTGCTTCTG
    TGGAACCATG CCACAGTGAC CACCTGCCAC TCCAAGACTG CCAATCTAAG CGAGGAGGTA
    AATAAAGGTG ACATCCTGGT GGTTGCAACT GGTCAGCCAG AAATGGTAAA AGGGGAGTGG
    ATCAAACCTG GGGCAATAGT CATCGACTGC GGGATCAATT ATATTCCAGA TGATACAAAA
    CCAAATGGGA GAAAAGTGGT GGGTGATGTG GCATACAATG AGGCCAAGGA GAGGGCGGGC
    TTCATCACTC CTGTTCCTGG CGGCGTGGGG CCCATGACGG TGGCAATGCT AATGCAGAGC
    ACAGTGGAGA GTGCGAAGCG TTTCCTGGAG AAATTTAAGC CAGGAAAGTG GATGATTCAA
    TATAACAACC TTAACCTCAA GACTCCTGTT CCAAGTGACA TTGATATATC ACGGTCTTGT
    AAGCCAAAGC CCATTGGCAG CCTGGCGCGG GAAATTGGTC TGCTCTCTGA AGAGGTGGAA
    TTATATGGTG ATACAAAGGC CAAGGTTCTG CTATCAGCAC TGGAACGCCT GAAGCACCAG
    CCTGATGGAA AATACGTGGT GGTGACTGGA ATCACCCCAA CGCCCCTGGG AGAAGGGAAA
    AGCACAACCA CCATTGGGCT GGTGCAGGCC CTTGGCGCCC ATCTTCATCA GAACGTCTTT
    GCATGTGTAC GACAGCCTTC TCAGGGCCCG ACCTTTGGAA TAAAAGGTGG CGCTGCAGGA
    GGTGGCTACT CACAGGTCAT TCCCATGGAA GAGTTTAATC TCCACCTCAC TGGTGACATC
    CATGCCATCA CTGCAGCGAA TAACCTTGTC GCCGCGGCCA TCGATGCCCG GATGTTCCAC
    GAACTGACGC AGACAGACAA GGCTCTCTTT AATCGTTTGG TACCATCAGT AAATGGAGTG
    AGAAAGTTCT CTGATATCCA AATCCGAAGA TTACAGAGAC TGGGTATTGA AAAAACTGAC
    CCTACCACAC TGACTGATGA CGAGATAAAC AGATTTGCAA GATTGGACAT TGATCCAGAA
    ACCATAACTT GGCAAAGAGT ATTGGATACC AACGATAGAT TCCTAAGGAA GATCACAATC
    GGACAGGCTC CAACAGAAAA AGGGCACACA CGGACGGCCC AGTTTGATAT CTCTGTGGCC
    AGTGAAATCA TGGCTGTGCT GGCTCTCACC AGTTCACTAG AAGACATGAG AGAGAGACTG
    AGCAAAATGG TGGTGGCATC CAGCAAGAAA GGGGAGCCTA TCAGTACCGA AGATCTGGGG
    GTGAGTGGTG CATTGACAGT GCTTATGAAG GATGCAATCA AGCCTAATCT CATGCAGACA
    TTAGAGGGCA CTCCAGTTTT CGTTCATGCT GGACCATTTG CCAACATCGC ACATGGGAAT
    TCCTCTATCA TTGCAGACCG GATTGCACTC AAGCTTGTTG GCCCTGAAGG GTTCGTGGTG
    ACTGAAGCAG GATTTGGAGC AGACATTGGA ATGGAAAAGT TTTTTAACAT CAAATGCCGG
    TATTCTGGTC TCCGCCCTCA TGTAGTGGTG CTTGTTGCCA CTGTCAGGGC TCTTAAAATG
    CACGGAGGCG GCCCCATGGT TACTGCTGGA CTGCCTCTTC CCGAGGCTTA CATAGTGGAG
    AACCTGGAGC TGGTTGAAAA AGGCTTCAGT AACTTAAAGA AACAAATTGA AAATGCCAGA
    ATGTTTGGAG TTCCTGTGGT CGTGGCCGTG AATGCATTCA AGACAGATAC GGAGGCCGAG
    CTGGACCTCG TCAGCCGCCT TGCCAAAGAA CATGGGGCTT TTGATGCTGT GAAATGCACG
    CACTGGGCAG AAGGGGGTAT GGGTGCCTTA GCCCTAGCTC AGGCTGTCCA GAGAGCAGCT
    CAGGCACCCA GCAGCTTCCA GCTCCTTTAT GACCTCAAGC TCCCAGTTGA GGATAAAATC
    AGGATCATCG CACAGAAAAT CTATGGGGCT GACGACATAG AATTGCTTCC TGAAGCTCAA
    CACAAAGCTG AAGTCTACAC GAAACAGGGC TTTGGGAACC TGCCTATCTG CATGGCCAAA
    ACACACTTGT CCTTGTCTCA TAACCCAGAG CAAAAGGGTG TGCCCACAGG CTTTGTCTTG
    CCCATTCGTG ACATCCGTGC CAGCGTCGGG GCCGGTTTTC TGTACCCCTT GGTAGGGACG
    ATGAGCACAA TGCCTGGACT CCCCACTCGG CCCTGTTTCT ATGATATTGA TTTGGACCCT
    GAAACTGAAC AAGTGAATGG GTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623549.1
    CDS96..2825
    Translation

    Target ORF information:

    RefSeq Version XM_005623492.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGAAC AAGTTCCTGG TTTTATACCA GGCCTGGCAA TTTTACAGGT TGGCGACAGA 
    GATGATTCTA ATCTTTATAT AAATGTGAAG CTGAAGGCTG CTGAAGAGAT TGGGATCAGA
    GCCACGCACA TTAAGTTACC AAAAACAGCC ACAGAATCTG AGGTATTAAA GTATGTTACA
    TCATTGAATG AAGACCTCAC TGTCCATGGT TTCATAGTGC AGCTGCCTTT AGATTCAGAG
    AACCCCATTA ACACTGAAGC AGTGATCAAT GCTATTGTAC CCGAAAAGGA TGTGGATGGG
    TTGACTAGCA TCAGTGCTGG AAAACTTGCT AGAGGTGATC TGAAAGACTG TTTCATCCCT
    TGTACACCTA AAGGATGCTT GGAACTAATC AAAGAGACAG GGGTACAGAT CGCTGGAAGG
    CACGCTGTAG TAGTTGGGCG CAGTAAGATA GTTGGTGCCC CGATGCATGA CCTGCTTCTG
    TGGAACCATG CCACAGTGAC CACCTGCCAC TCCAAGACTG CCAATCTAAG CGAGGAGGTA
    AATAAAGGTG ACATCCTGGT GGTTGCAACT GGTCAGCCAG AAATGGTAAA AGGGGAGTGG
    ATCAAACCTG GGGCAATAGT CATCGACTGC GGGATCAATT ATATTCCAGA TGATACAAAA
    CCAAATGGGA GAAAAGTGGT GGGTGATGTG GCATACAATG AGGCCAAGGA GAGGGCGGGC
    TTCATCACTC CTGTTCCTGG CGGCGTGGGG CCCATGACGG TGGCAATGCT AATGCAGAGC
    ACAGTGGAGA GTGCGAAGCG TTTCCTGGAG AAATTTAAGC CAGGAAAGTG GATGATTCAA
    TATAACAACC TTAACCTCAA GACTCCTGTT CCAAGTGACA TTGATATATC ACGGTCTTGT
    AAGCCAAAGC CCATTGGCAG CCTGGCGCGG GAAATTGGTC TGCTCTCTGA AGAGGTGGAA
    TTATATGGTG ATACAAAGGC CAAGGTTCTG CTATCAGCAC TGGAACGCCT GAAGCACCAG
    CCTGATGGAA AATACGTGGT GGTGACTGGA ATCACCCCAA CGCCCCTGGG AGAAGGGAAA
    AGCACAACCA CCATTGGGCT GGTGCAGGCC CTTGGCGCCC ATCTTCATCA GAACGTCTTT
    GCATGTGTAC GACAGCCTTC TCAGGGCCCG ACCTTTGGAA TAAAAGGTGG CGCTGCAGGA
    GGTGGCTACT CACAGGTCAT TCCCATGGAA GAGTTTAATC TCCACCTCAC TGGTGACATC
    CATGCCATCA CTGCAGCGAA TAACCTTGTC GCCGCGGCCA TCGATGCCCG GATGTTCCAC
    GAACTGACGC AGACAGACAA GGCTCTCTTT AATCGTTTGG TACCATCAGT AAATGGAGTG
    AGAAAGTTCT CTGATATCCA AATCCGAAGA TTACAGAGAC TGGGTATTGA AAAAACTGAC
    CCTACCACAC TGACTGATGA CGAGATAAAC AGATTTGCAA GATTGGACAT TGATCCAGAA
    ACCATAACTT GGCAAAGAGT ATTGGATACC AACGATAGAT TCCTAAGGAA GATCACAATC
    GGACAGGCTC CAACAGAAAA AGGGCACACA CGGACGGCCC AGTTTGATAT CTCTGTGGCC
    AGTGAAATCA TGGCTGTGCT GGCTCTCACC AGTTCACTAG AAGACATGAG AGAGAGACTG
    AGCAAAATGG TGGTGGCATC CAGCAAGAAA GGGGAGCCTA TCAGTACCGA AGATCTGGGG
    GTGAGTGGTG CATTGACAGT GCTTATGAAG GATGCAATCA AGCCTAATCT CATGCAGACA
    TTAGAGGGCA CTCCAGTTTT CGTTCATGCT GGACCATTTG CCAACATCGC ACATGGGAAT
    TCCTCTATCA TTGCAGACCG GATTGCACTC AAGCTTGTTG GCCCTGAAGG GTTCGTGGTG
    ACTGAAGCAG GATTTGGAGC AGACATTGGA ATGGAAAAGT TTTTTAACAT CAAATGCCGG
    TATTCTGGTC TCCGCCCTCA TGTAGTGGTG CTTGTTGCCA CTGTCAGGGC TCTTAAAATG
    CACGGAGGCG GCCCCATGGT TACTGCTGGA CTGCCTCTTC CCGAGGCTTA CATAGTGGAG
    AACCTGGAGC TGGTTGAAAA AGGCTTCAGT AACTTAAAGA AACAAATTGA AAATGCCAGA
    ATGTTTGGAG TTCCTGTGGT CGTGGCCGTG AATGCATTCA AGACAGATAC GGAGGCCGAG
    CTGGACCTCG TCAGCCGCCT TGCCAAAGAA CATGGGGCTT TTGATGCTGT GAAATGCACG
    CACTGGGCAG AAGGGGGTAT GGGTGCCTTA GCCCTAGCTC AGGCTGTCCA GAGAGCAGCT
    CAGGCACCCA GCAGCTTCCA GCTCCTTTAT GACCTCAAGC TCCCAGTTGA GGATAAAATC
    AGGATCATCG CACAGAAAAT CTATGGGGCT GACGACATAG AATTGCTTCC TGAAGCTCAA
    CACAAAGCTG AAGTCTACAC GAAACAGGGC TTTGGGAACC TGCCTATCTG CATGGCCAAA
    ACACACTTGT CCTTGTCTCA TAACCCAGAG CAAAAGGGTG TGCCCACAGG CTTTGTCTTG
    CCCATTCGTG ACATCCGTGC CAGCGTCGGG GCCGGTTTTC TGTACCCCTT GGTAGGGACG
    ATGAGCACAA TGCCTGGACT CCCCACTCGG CCCTGTTTCT ATGATATTGA TTTGGACCCT
    GAAACTGAAC AAGTGAATGG GTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10510
    Clone ID Related Accession (Same CDS sequence) XM_005623492.2 , XM_005623492.3
    Accession Version XM_005623492.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGAAC AAGTTCCTGG TTTTATACCA GGCCTGGCAA TTTTACAGGT TGGCGACAGA 
    GATGATTCTA ATCTTTATAT AAATGTGAAG CTGAAGGCTG CTGAAGAGAT TGGGATCAGA
    GCCACGCACA TTAAGTTACC AAAAACAGCC ACAGAATCTG AGGTATTAAA GTATGTTACA
    TCATTGAATG AAGACCTCAC TGTCCATGGT TTCATAGTGC AGCTGCCTTT AGATTCAGAG
    AACCCCATTA ACACTGAAGC AGTGATCAAT GCTATTGTAC CCGAAAAGGA TGTGGATGGG
    TTGACTAGCA TCAGTGCTGG AAAACTTGCT AGAGGTGATC TGAAAGACTG TTTCATCCCT
    TGTACACCTA AAGGATGCTT GGAACTAATC AAAGAGACAG GGGTACAGAT CGCTGGAAGG
    CACGCTGTAG TAGTTGGGCG CAGTAAGATA GTTGGTGCCC CGATGCATGA CCTGCTTCTG
    TGGAACCATG CCACAGTGAC CACCTGCCAC TCCAAGACTG CCAATCTAAG CGAGGAGGTA
    AATAAAGGTG ACATCCTGGT GGTTGCAACT GGTCAGCCAG AAATGGTAAA AGGGGAGTGG
    ATCAAACCTG GGGCAATAGT CATCGACTGC GGGATCAATT ATATTCCAGA TGATACAAAA
    CCAAATGGGA GAAAAGTGGT GGGTGATGTG GCATACAATG AGGCCAAGGA GAGGGCGGGC
    TTCATCACTC CTGTTCCTGG CGGCGTGGGG CCCATGACGG TGGCAATGCT AATGCAGAGC
    ACAGTGGAGA GTGCGAAGCG TTTCCTGGAG AAATTTAAGC CAGGAAAGTG GATGATTCAA
    TATAACAACC TTAACCTCAA GACTCCTGTT CCAAGTGACA TTGATATATC ACGGTCTTGT
    AAGCCAAAGC CCATTGGCAG CCTGGCGCGG GAAATTGGTC TGCTCTCTGA AGAGGTGGAA
    TTATATGGTG ATACAAAGGC CAAGGTTCTG CTATCAGCAC TGGAACGCCT GAAGCACCAG
    CCTGATGGAA AATACGTGGT GGTGACTGGA ATCACCCCAA CGCCCCTGGG AGAAGGGAAA
    AGCACAACCA CCATTGGGCT GGTGCAGGCC CTTGGCGCCC ATCTTCATCA GAACGTCTTT
    GCATGTGTAC GACAGCCTTC TCAGGGCCCG ACCTTTGGAA TAAAAGGTGG CGCTGCAGGA
    GGTGGCTACT CACAGGTCAT TCCCATGGAA GAGTTTAATC TCCACCTCAC TGGTGACATC
    CATGCCATCA CTGCAGCGAA TAACCTTGTC GCCGCGGCCA TCGATGCCCG GATGTTCCAC
    GAACTGACGC AGACAGACAA GGCTCTCTTT AATCGTTTGG TACCATCAGT AAATGGAGTG
    AGAAAGTTCT CTGATATCCA AATCCGAAGA TTACAGAGAC TGGGTATTGA AAAAACTGAC
    CCTACCACAC TGACTGATGA CGAGATAAAC AGATTTGCAA GATTGGACAT TGATCCAGAA
    ACCATAACTT GGCAAAGAGT ATTGGATACC AACGATAGAT TCCTAAGGAA GATCACAATC
    GGACAGGCTC CAACAGAAAA AGGGCACACA CGGACGGCCC AGTTTGATAT CTCTGTGGCC
    AGTGAAATCA TGGCTGTGCT GGCTCTCACC AGTTCACTAG AAGACATGAG AGAGAGACTG
    AGCAAAATGG TGGTGGCATC CAGCAAGAAA GGGGAGCCTA TCAGTACCGA AGATCTGGGG
    GTGAGTGGTG CATTGACAGT GCTTATGAAG GATGCAATCA AGCCTAATCT CATGCAGACA
    TTAGAGGGCA CTCCAGTTTT CGTTCATGCT GGACCATTTG CCAACATCGC ACATGGGAAT
    TCCTCTATCA TTGCAGACCG GATTGCACTC AAGCTTGTTG GCCCTGAAGG GTTCGTGGTG
    ACTGAAGCAG GATTTGGAGC AGACATTGGA ATGGAAAAGT TTTTTAACAT CAAATGCCGG
    TATTCTGGTC TCCGCCCTCA TGTAGTGGTG CTTGTTGCCA CTGTCAGGGC TCTTAAAATG
    CACGGAGGCG GCCCCATGGT TACTGCTGGA CTGCCTCTTC CCGAGGCTTA CATAGTGGAG
    AACCTGGAGC TGGTTGAAAA AGGCTTCAGT AACTTAAAGA AACAAATTGA AAATGCCAGA
    ATGTTTGGAG TTCCTGTGGT CGTGGCCGTG AATGCATTCA AGACAGATAC GGAGGCCGAG
    CTGGACCTCG TCAGCCGCCT TGCCAAAGAA CATGGGGCTT TTGATGCTGT GAAATGCACG
    CACTGGGCAG AAGGGGGTAT GGGTGCCTTA GCCCTAGCTC AGGCTGTCCA GAGAGCAGCT
    CAGGCACCCA GCAGCTTCCA GCTCCTTTAT GACCTCAAGC TCCCAGTTGA GGATAAAATC
    AGGATCATCG CACAGAAAAT CTATGGGGCT GACGACATAG AATTGCTTCC TGAAGCTCAA
    CACAAAGCTG AAGTCTACAC GAAACAGGGC TTTGGGAACC TGCCTATCTG CATGGCCAAA
    ACACACTTGT CCTTGTCTCA TAACCCAGAG CAAAAGGGTG TGCCCACAGG CTTTGTCTTG
    CCCATTCGTG ACATCCGTGC CAGCGTCGGG GCCGGTTTTC TGTACCCCTT GGTAGGGACG
    ATGAGCACAA TGCCTGGACT CCCCACTCGG CCCTGTTTCT ATGATATTGA TTTGGACCCT
    GAAACTGAAC AAGTGAATGG GTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623549.1
    CDS57..2786
    Translation

    Target ORF information:

    RefSeq Version XM_005623492.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623492.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGAAC AAGTTCCTGG TTTTATACCA GGCCTGGCAA TTTTACAGGT TGGCGACAGA 
    GATGATTCTA ATCTTTATAT AAATGTGAAG CTGAAGGCTG CTGAAGAGAT TGGGATCAGA
    GCCACGCACA TTAAGTTACC AAAAACAGCC ACAGAATCTG AGGTATTAAA GTATGTTACA
    TCATTGAATG AAGACCTCAC TGTCCATGGT TTCATAGTGC AGCTGCCTTT AGATTCAGAG
    AACCCCATTA ACACTGAAGC AGTGATCAAT GCTATTGTAC CCGAAAAGGA TGTGGATGGG
    TTGACTAGCA TCAGTGCTGG AAAACTTGCT AGAGGTGATC TGAAAGACTG TTTCATCCCT
    TGTACACCTA AAGGATGCTT GGAACTAATC AAAGAGACAG GGGTACAGAT CGCTGGAAGG
    CACGCTGTAG TAGTTGGGCG CAGTAAGATA GTTGGTGCCC CGATGCATGA CCTGCTTCTG
    TGGAACCATG CCACAGTGAC CACCTGCCAC TCCAAGACTG CCAATCTAAG CGAGGAGGTA
    AATAAAGGTG ACATCCTGGT GGTTGCAACT GGTCAGCCAG AAATGGTAAA AGGGGAGTGG
    ATCAAACCTG GGGCAATAGT CATCGACTGC GGGATCAATT ATATTCCAGA TGATACAAAA
    CCAAATGGGA GAAAAGTGGT GGGTGATGTG GCATACAATG AGGCCAAGGA GAGGGCGGGC
    TTCATCACTC CTGTTCCTGG CGGCGTGGGG CCCATGACGG TGGCAATGCT AATGCAGAGC
    ACAGTGGAGA GTGCGAAGCG TTTCCTGGAG AAATTTAAGC CAGGAAAGTG GATGATTCAA
    TATAACAACC TTAACCTCAA GACTCCTGTT CCAAGTGACA TTGATATATC ACGGTCTTGT
    AAGCCAAAGC CCATTGGCAG CCTGGCGCGG GAAATTGGTC TGCTCTCTGA AGAGGTGGAA
    TTATATGGTG ATACAAAGGC CAAGGTTCTG CTATCAGCAC TGGAACGCCT GAAGCACCAG
    CCTGATGGAA AATACGTGGT GGTGACTGGA ATCACCCCAA CGCCCCTGGG AGAAGGGAAA
    AGCACAACCA CCATTGGGCT GGTGCAGGCC CTTGGCGCCC ATCTTCATCA GAACGTCTTT
    GCATGTGTAC GACAGCCTTC TCAGGGCCCG ACCTTTGGAA TAAAAGGTGG CGCTGCAGGA
    GGTGGCTACT CACAGGTCAT TCCCATGGAA GAGTTTAATC TCCACCTCAC TGGTGACATC
    CATGCCATCA CTGCAGCGAA TAACCTTGTC GCCGCGGCCA TCGATGCCCG GATGTTCCAC
    GAACTGACGC AGACAGACAA GGCTCTCTTT AATCGTTTGG TACCATCAGT AAATGGAGTG
    AGAAAGTTCT CTGATATCCA AATCCGAAGA TTACAGAGAC TGGGTATTGA AAAAACTGAC
    CCTACCACAC TGACTGATGA CGAGATAAAC AGATTTGCAA GATTGGACAT TGATCCAGAA
    ACCATAACTT GGCAAAGAGT ATTGGATACC AACGATAGAT TCCTAAGGAA GATCACAATC
    GGACAGGCTC CAACAGAAAA AGGGCACACA CGGACGGCCC AGTTTGATAT CTCTGTGGCC
    AGTGAAATCA TGGCTGTGCT GGCTCTCACC AGTTCACTAG AAGACATGAG AGAGAGACTG
    AGCAAAATGG TGGTGGCATC CAGCAAGAAA GGGGAGCCTA TCAGTACCGA AGATCTGGGG
    GTGAGTGGTG CATTGACAGT GCTTATGAAG GATGCAATCA AGCCTAATCT CATGCAGACA
    TTAGAGGGCA CTCCAGTTTT CGTTCATGCT GGACCATTTG CCAACATCGC ACATGGGAAT
    TCCTCTATCA TTGCAGACCG GATTGCACTC AAGCTTGTTG GCCCTGAAGG GTTCGTGGTG
    ACTGAAGCAG GATTTGGAGC AGACATTGGA ATGGAAAAGT TTTTTAACAT CAAATGCCGG
    TATTCTGGTC TCCGCCCTCA TGTAGTGGTG CTTGTTGCCA CTGTCAGGGC TCTTAAAATG
    CACGGAGGCG GCCCCATGGT TACTGCTGGA CTGCCTCTTC CCGAGGCTTA CATAGTGGAG
    AACCTGGAGC TGGTTGAAAA AGGCTTCAGT AACTTAAAGA AACAAATTGA AAATGCCAGA
    ATGTTTGGAG TTCCTGTGGT CGTGGCCGTG AATGCATTCA AGACAGATAC GGAGGCCGAG
    CTGGACCTCG TCAGCCGCCT TGCCAAAGAA CATGGGGCTT TTGATGCTGT GAAATGCACG
    CACTGGGCAG AAGGGGGTAT GGGTGCCTTA GCCCTAGCTC AGGCTGTCCA GAGAGCAGCT
    CAGGCACCCA GCAGCTTCCA GCTCCTTTAT GACCTCAAGC TCCCAGTTGA GGATAAAATC
    AGGATCATCG CACAGAAAAT CTATGGGGCT GACGACATAG AATTGCTTCC TGAAGCTCAA
    CACAAAGCTG AAGTCTACAC GAAACAGGGC TTTGGGAACC TGCCTATCTG CATGGCCAAA
    ACACACTTGT CCTTGTCTCA TAACCCAGAG CAAAAGGGTG TGCCCACAGG CTTTGTCTTG
    CCCATTCGTG ACATCCGTGC CAGCGTCGGG GCCGGTTTTC TGTACCCCTT GGTAGGGACG
    ATGAGCACAA TGCCTGGACT CCCCACTCGG CCCTGTTTCT ATGATATTGA TTTGGACCCT
    GAAACTGAAC AAGTGAATGG GTTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.