MTHFD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD1
Entrez Gene ID	534382
Full Name	methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 Names C-1-tetrahydrofolate synthase, cytoplasmic methylenetetrahydrofolate dehydrogenase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005211997.2	XP_005212054.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_024997662.1	XP_024853430.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
NM_001083477.1	NP_001076946.1	C-1-tetrahydrofolate synthase, cytoplasmic
XM_024997663.1	XP_024853431.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
XM_024997664.1	XP_024853432.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Neurospora crassa	NCU03877	XP_956550.1
	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTHFD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37209	NM_001083477.1 Latest version!	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa23501	XM_005211997.2 Latest version!	Bos taurus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa23501	XM_024997662.1 Latest version!	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa219636	XM_024997663.1 Latest version!	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa219637	XM_024997664.1 Latest version!	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD1 ( NM_001083477.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001076946.1 Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Price & Availability↑

CloneID	OBa37209
Clone ID Related Accession (Same CDS sequence)	NM_001083477.1
Accession Version	NM_001083477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114711.1. On Mar 27, 2007 this sequence version replaced XM_614133.3. ##Evidence-Data-START## Transcript exon combination :: BC114711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAA GGAGAGACTG 
AAGAACCAAG TCATTCAGAT GAAGGAGGAA GTACCTGGTT TCATACCAGG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGACTCTAAT CTTTATATAA ATATGAAGTT GAAGGCTGCT 
GAGGAGATCG GGATCAAAGC CACGCACATT AAGTTACCCC GGACGGCCAC GGAATCTGAG 
GTGTTAAAGT GCATCACATC ATTGAACGAA GACCTCACGG TGCACGGCTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAACGT TGGTCAATGC TATCGCCCCC 
GAAAAAGATG TGGATGGATT GAATAGCATC AGTGCTGGAA AACTTGCTAG AGGGGACCTT 
AGTAACTGTT TCATCCCTTG TACACCCAAA GGATGCTTGG AACTCATCAA GGAGACAGGG 
GTGCAAATTG CTGGAAGGCA CGCTGTGGTG GTCGGGCGCA GTAAAATCGT TGGTGCCCCA 
ATGCATGACT TGCTTCTATG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGAAAG AGGAGATAAG TAAAGGTGAC ATCCTGGTGG TTGCAGCTGG CCAACCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCGATAGTCA TCGACTGTGG AATCAATTAC 
GTCACAGATG ATACAAAACC AAATGGGAAG AAAATTGTGG GTGATGTAGC ATACACGGAA 
GCCAAGGAGA GGGCAAGCTT CATCACCCCC GTTCCTGGTG GCGTCGGGCC CATGACAGTG 
GCGATGCTAA TGCAGAGCAC AGTAGAGAGT GCAAAGCGTT TCTTGGAGAA CTTCAAGCCA 
GGAAAGTGGA TCATTCAGTA TAACAAACTT AACCTGAAGA CACCCGTGCC AAGTGACATT 
GATATATCAC GGTCTTACAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AGTTGGTCTG 
CGCACTGAAG AGGTAGAATT GTATGGTGAA ACCAAGGCTA AAGTTCTGCT GTCGGCGCTG 
GAACGGCTGA AGCACCAGCC TGATGGGAAA TACGTGGTGG TAACTGGAAT CACTCCAACA 
CCCCTGGGAG AGGGGAAAAG CACAACCACG ATTGGGTTGG TGCAGGCCCT TGGCACCCAT 
CTCCATCAGA ATGTCTTTGC ATGTGTGCGA CAGCCTTCTC AGGGGCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGCGG TGGCTACTCT CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTGGC CGCAGCCATC 
GATGCCCGTA TGTTCCATGA AGAGACCCAG ACAGACAAGG CTCTTTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCC GATATCCAAA TCCGGAGGTT GCGGAGACTA 
GGCATTGAAA AGACTGACCC TGCCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC TATCACTTGG CAAAGAGTGC TGGATACCAA CGATAGATTC 
CTGAGGAAGA TCACAATCGG ACAGGCTCCA ACGGAGAAGG GGCACTCACG GACGGCCCAG 
TTTGATATCT CCGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCGTCCA GCAAGAAAGG GGAGCCCATC 
AGTACTGAGG ATCTGGGAGT GAGTGGAGCA TTGACGGTCC TTATGAAGGA CGCCATCAAG 
CCCAATCTCA TGCAGACGTT AGAGGGCACT CCAGTGTTTG TTCACGCTGG GCCGTTTGCC 
AACATCGCAC ACGGGAATTC CTCCATCATT GCAGACAGGA TTGCACTCAA GCTTGTCGGT 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCAG ACATTGGAAT GGAAAAGTTC 
TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCGCACG TGGTGGTGCT TGTCGCCACT 
GTCAGGGCTC TGAAAATGCA CGGAGGTGGC CCCACGGTTA CTGCTGGACT TCCGCTTCCC 
AAGGCCTACA TAGAGGAGGA CCTGGAGCTG GTTGGAAAAG GCTTCAGTAA CTTGAAGAAG 
CAAATTGAAA ATGCCAGGAT GTTTGGCGTG CCAGTGGTAG TGGCTGTGAA CGCCTTCAAG 
ACAGATACGG AGGCCGAGCT GGACCTTGTC AGCCGCCTCG CCAAAGAACA TGGTGCTTTT 
GACGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GCGCCCTGGC GCTGGCTGAG 
GCCGTCCAGA GGGCAGCTGC GGCCCCCAGC AGCTTCCAGC TTCTCTATGA CCTCAAGCTC 
CCAGTTGAGG ACAAAATCAG AATTATTGCG CAGAAGATCT ATGGAGCTGA CAACATCGAA 
TTGCTCCCTG AAGCCCAGCA CAAAGCAGAG GTCTACACAA AGCAGGGCTT TGGGAATCTG 
CCCATCTGCA TGGCCAAGAC ACACTTGTCC TTGTCTCACA ACCCAGAGCT AAAAGGTGTG 
CCCACTGGCT TCGTCCTGCC CATTCGCGAC ATCCGCGCCA GCGTGGGGGC CGGCTTTCTG 
TACCCTCTGG TGGGAACGAT GAGCACCATG CCTGGACTCC CTACTCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACTGAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076946.1
CDS	17..2824
Translation	MAPAEILNGKVVSAQIKERLKNQVIQMKEEVPGFIPGLAILQVGNRDDSNLYINMKLKAAEEIGIKATHIKLPRTATESEVLKCITSLNEDLTVHGFIVQLPLDSENPINTETLVNAIAPEKDVDGLNSISAGKLARGDLSNCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINYVTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKPGKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSALERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPATLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHSRTAQFDISVASEIMAVLALTSSLEDMRERLGKMVVASSKKGEPISTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEEDLELVGKGFSNLKKQIENARMFGVPVVVAVNAFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALAEAVQRAAAAPSSFQLLYDLKLPVEDKIRIIAQKIYGADNIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	NM_001083477.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083477.1

ORF Insert Sequence:

ATGGCGCCGG CGGAAATCCT GAACGGGAAG GTGGTCTCCG CGCAAATAAA GGAGAGACTG 
AAGAACCAAG TCATTCAGAT GAAGGAGGAA GTACCTGGTT TCATACCAGG CCTGGCAATT 
TTACAGGTTG GCAACAGAGA TGACTCTAAT CTTTATATAA ATATGAAGTT GAAGGCTGCT 
GAGGAGATCG GGATCAAAGC CACGCACATT AAGTTACCCC GGACGGCCAC GGAATCTGAG 
GTGTTAAAGT GCATCACATC ATTGAACGAA GACCTCACGG TGCACGGCTT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TCCCATTAAC ACTGAAACGT TGGTCAATGC TATCGCCCCC 
GAAAAAGATG TGGATGGATT GAATAGCATC AGTGCTGGAA AACTTGCTAG AGGGGACCTT 
AGTAACTGTT TCATCCCTTG TACACCCAAA GGATGCTTGG AACTCATCAA GGAGACAGGG 
GTGCAAATTG CTGGAAGGCA CGCTGTGGTG GTCGGGCGCA GTAAAATCGT TGGTGCCCCA 
ATGCATGACT TGCTTCTATG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGAAAG AGGAGATAAG TAAAGGTGAC ATCCTGGTGG TTGCAGCTGG CCAACCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCGATAGTCA TCGACTGTGG AATCAATTAC 
GTCACAGATG ATACAAAACC AAATGGGAAG AAAATTGTGG GTGATGTAGC ATACACGGAA 
GCCAAGGAGA GGGCAAGCTT CATCACCCCC GTTCCTGGTG GCGTCGGGCC CATGACAGTG 
GCGATGCTAA TGCAGAGCAC AGTAGAGAGT GCAAAGCGTT TCTTGGAGAA CTTCAAGCCA 
GGAAAGTGGA TCATTCAGTA TAACAAACTT AACCTGAAGA CACCCGTGCC AAGTGACATT 
GATATATCAC GGTCTTACAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AGTTGGTCTG 
CGCACTGAAG AGGTAGAATT GTATGGTGAA ACCAAGGCTA AAGTTCTGCT GTCGGCGCTG 
GAACGGCTGA AGCACCAGCC TGATGGGAAA TACGTGGTGG TAACTGGAAT CACTCCAACA 
CCCCTGGGAG AGGGGAAAAG CACAACCACG ATTGGGTTGG TGCAGGCCCT TGGCACCCAT 
CTCCATCAGA ATGTCTTTGC ATGTGTGCGA CAGCCTTCTC AGGGGCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGCGG TGGCTACTCT CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTGGC CGCAGCCATC 
GATGCCCGTA TGTTCCATGA AGAGACCCAG ACAGACAAGG CTCTTTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCC GATATCCAAA TCCGGAGGTT GCGGAGACTA 
GGCATTGAAA AGACTGACCC TGCCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC TATCACTTGG CAAAGAGTGC TGGATACCAA CGATAGATTC 
CTGAGGAAGA TCACAATCGG ACAGGCTCCA ACGGAGAAGG GGCACTCACG GACGGCCCAG 
TTTGATATCT CCGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCGTCCA GCAAGAAAGG GGAGCCCATC 
AGTACTGAGG ATCTGGGAGT GAGTGGAGCA TTGACGGTCC TTATGAAGGA CGCCATCAAG 
CCCAATCTCA TGCAGACGTT AGAGGGCACT CCAGTGTTTG TTCACGCTGG GCCGTTTGCC 
AACATCGCAC ACGGGAATTC CTCCATCATT GCAGACAGGA TTGCACTCAA GCTTGTCGGT 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCAG ACATTGGAAT GGAAAAGTTC 
TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCGCACG TGGTGGTGCT TGTCGCCACT 
GTCAGGGCTC TGAAAATGCA CGGAGGTGGC CCCACGGTTA CTGCTGGACT TCCGCTTCCC 
AAGGCCTACA TAGAGGAGGA CCTGGAGCTG GTTGGAAAAG GCTTCAGTAA CTTGAAGAAG 
CAAATTGAAA ATGCCAGGAT GTTTGGCGTG CCAGTGGTAG TGGCTGTGAA CGCCTTCAAG 
ACAGATACGG AGGCCGAGCT GGACCTTGTC AGCCGCCTCG CCAAAGAACA TGGTGCTTTT 
GACGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GCGCCCTGGC GCTGGCTGAG 
GCCGTCCAGA GGGCAGCTGC GGCCCCCAGC AGCTTCCAGC TTCTCTATGA CCTCAAGCTC 
CCAGTTGAGG ACAAAATCAG AATTATTGCG CAGAAGATCT ATGGAGCTGA CAACATCGAA 
TTGCTCCCTG AAGCCCAGCA CAAAGCAGAG GTCTACACAA AGCAGGGCTT TGGGAATCTG 
CCCATCTGCA TGGCCAAGAC ACACTTGTCC TTGTCTCACA ACCCAGAGCT AAAAGGTGTG 
CCCACTGGCT TCGTCCTGCC CATTCGCGAC ATCCGCGCCA GCGTGGGGGC CGGCTTTCTG 
TACCCTCTGG TGGGAACGAT GAGCACCATG CCTGGACTCC CTACTCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACTGAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_005211997.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212054.1 Bos taurus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23501
Clone ID Related Accession (Same CDS sequence)	XM_005211997.2 , XM_024997662.1
Accession Version	XM_005211997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC AAATAAAGGA GAGACTGAAG AACCAAGTCA TTCAGATGAA GGAGGAAGTA 
CCTGGTTTCA TACCAGGCCT GGCAATTTTA CAGGTTGGCA ACAGAGATGA CTCTAATCTT 
TATATAAATA TGAAGTTGAA GGCTGCTGAG GAGATCGGGA TCAAAGCCAC GCACATTAAG 
TTACCCCGGA CGGCCACGGA ATCTGAGGTG TTAAAGTGCA TCACATCATT GAACGAAGAC 
CTCACGGTGC ACGGCTTCAT AGTGCAGCTG CCTTTAGATT CAGAGAATCC CATTAACACT 
GAAACGTTGG TCAATGCTAT CGCCCCCGAA AAAGATGTGG ATGGATTGAA TAGCATCAGT 
GCTGGAAAAC TTGCTAGAGG GGACCTTAGT AACTGTTTCA TCCCTTGTAC ACCCAAAGGA 
TGCTTGGAAC TCATCAAGGA GACAGGGGTG CAAATTGCTG GAAGGCACGC TGTGGTGGTC 
GGGCGCAGTA AAATCGTTGG TGCCCCAATG CATGACTTGC TTCTATGGAA CCATGCCACA 
GTGACCACCT GCCACTCCAA GACTGCCAAT CTGAAAGAGG AGATAAGTAA AGGTGACATC 
CTGGTGGTTG CAGCTGGCCA ACCTGAAATG GTGAAAGGGG AGTGGATCAA ACCTGGGGCG 
ATAGTCATCG ACTGTGGAAT CAATTACGTC ACAGATGATA CAAAACCAAA TGGGAAGAAA 
ATTGTGGGTG ATGTAGCATA CACGGAAGCC AAGGAGAGGG CAAGCTTCAT CACCCCCGTT 
CCTGGTGGCG TCGGGCCCAT GACAGTGGCG ATGCTAATGC AGAGCACAGT AGAGAGTGCA 
AAGCGTTTCT TGGAGAACTT CAAGCCAGGA AAGTGGATCA TTCAGTATAA CAAACTTAAC 
CTGAAGACAC CCGTGCCAAG TGACATTGAT ATATCACGGT CTTACAAGCC AAAGCCCATT 
GGTAACCTGG CTCGAGAAGT TGGTCTGCGC ACTGAAGAGG TAGAATTGTA TGGTGAAACC 
AAGGCTAAAG TTCTGCTGTC GGCGCTGGAA CGGCTGAAGC ACCAGCCTGA TGGGAAATAC 
GTGGTGGTAA CTGGAATCAC TCCAACACCC CTGGGAGAGG GGAAAAGCAC AACCACGATT 
GGGTTGGTGC AGGCCCTTGG CACCCATCTC CATCAGAATG TCTTTGCATG TGTGCGACAG 
CCTTCTCAGG GGCCCACCTT TGGGATAAAA GGTGGCGCTG CAGGCGGTGG CTACTCTCAG 
GTCATTCCTA TGGAAGAGTT TAATCTCCAC CTCACTGGTG ACATCCATGC CATCACTGCA 
GCTAATAACC TTGTGGCCGC AGCCATCGAT GCCCGTATGT TCCATGAAGA GACCCAGACA 
GACAAGGCTC TTTTTAATCG TTTGGTGCCA TCAGTAAATG GAGTGAGAAA GTTCTCCGAT 
ATCCAAATCC GGAGGTTGCG GAGACTAGGC ATTGAAAAGA CTGACCCTGC CACACTGACA 
GATGAAGAGA TAAACAGATT TGCAAGATTG GACATTGATC CAGAAACTAT CACTTGGCAA 
AGAGTGCTGG ATACCAACGA TAGATTCCTG AGGAAGATCA CAATCGGACA GGCTCCAACG 
GAGAAGGGGC ACTCACGGAC GGCCCAGTTT GATATCTCCG TGGCCAGTGA AATCATGGCT 
GTCCTGGCTC TCACCAGTTC TCTAGAAGAC ATGAGAGAGA GACTGGGCAA AATGGTGGTG 
GCGTCCAGCA AGAAAGGGGA GCCCATCAGT ACTGAGGATC TGGGAGTGAG TGGAGCATTG 
ACGGTCCTTA TGAAGGACGC CATCAAGCCC AATCTCATGC AGACGTTAGA GGGCACTCCA 
GTGTTTGTTC ACGCTGGGCC GTTTGCCAAC ATCGCACACG GGAATTCCTC CATCATTGCA 
GACAGGATTG CACTCAAGCT TGTCGGTCCC GAAGGGTTTG TAGTGACTGA AGCAGGATTC 
GGAGCAGACA TTGGAATGGA AAAGTTCTTT AACATCAAAT GCCGGTATTC TGGTCTCCGC 
CCGCACGTGG TGGTGCTTGT CGCCACTGTC AGGGCTCTGA AAATGCACGG AGGTGGCCCC 
ACGGTTACTG CTGGACTTCC GCTTCCCAAG GCCTACATAG AGGAGGACCT GGAGCTGGTT 
GGAAAAGGCT TCAGTAACTT GAAGAAGCAA ATTGAAAATG CCAGGATGTT TGGCGTGCCA 
GTGGTAGTGG CTGTGAACGC CTTCAAGACA GATACGGAGG CCGAGCTGGA CCTTGTCAGC 
CGCCTCGCCA AAGAACATGG TGCTTTTGAC GCCGTGAAGT GCACTCACTG GGCAGAAGGG 
GGCAAGGGCG CCCTGGCGCT GGCTGAGGCC GTCCAGAGGG CAGCTGCGGC CCCCAGCAGC 
TTCCAGCTTC TCTATGACCT CAAGCTCCCA GTTGAGGACA AAATCAGAAT TATTGCGCAG 
AAGATCTATG GAGCTGACAA CATCGAATTG CTCCCTGAAG CCCAGCACAA AGCAGAGGTC 
TACACAAAGC AGGGCTTTGG GAATCTGCCC ATCTGCATGG CCAAGACACA CTTGTCCTTG 
TCTCACAACC CAGAGCTAAA AGGTGTGCCC ACTGGCTTCG TCCTGCCCAT TCGCGACATC 
CGCGCCAGCG TGGGGGCCGG CTTTCTGTAC CCTCTGGTGG GAACGATGAG CACCATGCCT 
GGACTCCCTA CTCGGCCCTG TTTCTATGAT ATTGATTTGG ACCCTGAAAC TGAGCAGGTG 
AATGGGCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212054.1
CDS	16..2790
Translation	MERQIKERLKNQVIQMKEEVPGFIPGLAILQVGNRDDSNLYINMKLKAAEEIGIKATHIKLPRTATESEVLKCITSLNEDLTVHGFIVQLPLDSENPINTETLVNAIAPEKDVDGLNSISAGKLARGDLSNCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINYVTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKPGKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSALERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPATLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHSRTAQFDISVASEIMAVLALTSSLEDMRERLGKMVVASSKKGEPISTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEEDLELVGKGFSNLKKQIENARMFGVPVVVAVNAFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALAEAVQRAAAAPSSFQLLYDLKLPVEDKIRIIAQKIYGADNIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_005211997.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211997.2

ORF Insert Sequence:

ATGGAACGGC AAATAAAGGA GAGACTGAAG AACCAAGTCA TTCAGATGAA GGAGGAAGTA 
CCTGGTTTCA TACCAGGCCT GGCAATTTTA CAGGTTGGCA ACAGAGATGA CTCTAATCTT 
TATATAAATA TGAAGTTGAA GGCTGCTGAG GAGATCGGGA TCAAAGCCAC GCACATTAAG 
TTACCCCGGA CGGCCACGGA ATCTGAGGTG TTAAAGTGCA TCACATCATT GAACGAAGAC 
CTCACGGTGC ACGGCTTCAT AGTGCAGCTG CCTTTAGATT CAGAGAATCC CATTAACACT 
GAAACGTTGG TCAATGCTAT CGCCCCCGAA AAAGATGTGG ATGGATTGAA TAGCATCAGT 
GCTGGAAAAC TTGCTAGAGG GGACCTTAGT AACTGTTTCA TCCCTTGTAC ACCCAAAGGA 
TGCTTGGAAC TCATCAAGGA GACAGGGGTG CAAATTGCTG GAAGGCACGC TGTGGTGGTC 
GGGCGCAGTA AAATCGTTGG TGCCCCAATG CATGACTTGC TTCTATGGAA CCATGCCACA 
GTGACCACCT GCCACTCCAA GACTGCCAAT CTGAAAGAGG AGATAAGTAA AGGTGACATC 
CTGGTGGTTG CAGCTGGCCA ACCTGAAATG GTGAAAGGGG AGTGGATCAA ACCTGGGGCG 
ATAGTCATCG ACTGTGGAAT CAATTACGTC ACAGATGATA CAAAACCAAA TGGGAAGAAA 
ATTGTGGGTG ATGTAGCATA CACGGAAGCC AAGGAGAGGG CAAGCTTCAT CACCCCCGTT 
CCTGGTGGCG TCGGGCCCAT GACAGTGGCG ATGCTAATGC AGAGCACAGT AGAGAGTGCA 
AAGCGTTTCT TGGAGAACTT CAAGCCAGGA AAGTGGATCA TTCAGTATAA CAAACTTAAC 
CTGAAGACAC CCGTGCCAAG TGACATTGAT ATATCACGGT CTTACAAGCC AAAGCCCATT 
GGTAACCTGG CTCGAGAAGT TGGTCTGCGC ACTGAAGAGG TAGAATTGTA TGGTGAAACC 
AAGGCTAAAG TTCTGCTGTC GGCGCTGGAA CGGCTGAAGC ACCAGCCTGA TGGGAAATAC 
GTGGTGGTAA CTGGAATCAC TCCAACACCC CTGGGAGAGG GGAAAAGCAC AACCACGATT 
GGGTTGGTGC AGGCCCTTGG CACCCATCTC CATCAGAATG TCTTTGCATG TGTGCGACAG 
CCTTCTCAGG GGCCCACCTT TGGGATAAAA GGTGGCGCTG CAGGCGGTGG CTACTCTCAG 
GTCATTCCTA TGGAAGAGTT TAATCTCCAC CTCACTGGTG ACATCCATGC CATCACTGCA 
GCTAATAACC TTGTGGCCGC AGCCATCGAT GCCCGTATGT TCCATGAAGA GACCCAGACA 
GACAAGGCTC TTTTTAATCG TTTGGTGCCA TCAGTAAATG GAGTGAGAAA GTTCTCCGAT 
ATCCAAATCC GGAGGTTGCG GAGACTAGGC ATTGAAAAGA CTGACCCTGC CACACTGACA 
GATGAAGAGA TAAACAGATT TGCAAGATTG GACATTGATC CAGAAACTAT CACTTGGCAA 
AGAGTGCTGG ATACCAACGA TAGATTCCTG AGGAAGATCA CAATCGGACA GGCTCCAACG 
GAGAAGGGGC ACTCACGGAC GGCCCAGTTT GATATCTCCG TGGCCAGTGA AATCATGGCT 
GTCCTGGCTC TCACCAGTTC TCTAGAAGAC ATGAGAGAGA GACTGGGCAA AATGGTGGTG 
GCGTCCAGCA AGAAAGGGGA GCCCATCAGT ACTGAGGATC TGGGAGTGAG TGGAGCATTG 
ACGGTCCTTA TGAAGGACGC CATCAAGCCC AATCTCATGC AGACGTTAGA GGGCACTCCA 
GTGTTTGTTC ACGCTGGGCC GTTTGCCAAC ATCGCACACG GGAATTCCTC CATCATTGCA 
GACAGGATTG CACTCAAGCT TGTCGGTCCC GAAGGGTTTG TAGTGACTGA AGCAGGATTC 
GGAGCAGACA TTGGAATGGA AAAGTTCTTT AACATCAAAT GCCGGTATTC TGGTCTCCGC 
CCGCACGTGG TGGTGCTTGT CGCCACTGTC AGGGCTCTGA AAATGCACGG AGGTGGCCCC 
ACGGTTACTG CTGGACTTCC GCTTCCCAAG GCCTACATAG AGGAGGACCT GGAGCTGGTT 
GGAAAAGGCT TCAGTAACTT GAAGAAGCAA ATTGAAAATG CCAGGATGTT TGGCGTGCCA 
GTGGTAGTGG CTGTGAACGC CTTCAAGACA GATACGGAGG CCGAGCTGGA CCTTGTCAGC 
CGCCTCGCCA AAGAACATGG TGCTTTTGAC GCCGTGAAGT GCACTCACTG GGCAGAAGGG 
GGCAAGGGCG CCCTGGCGCT GGCTGAGGCC GTCCAGAGGG CAGCTGCGGC CCCCAGCAGC 
TTCCAGCTTC TCTATGACCT CAAGCTCCCA GTTGAGGACA AAATCAGAAT TATTGCGCAG 
AAGATCTATG GAGCTGACAA CATCGAATTG CTCCCTGAAG CCCAGCACAA AGCAGAGGTC 
TACACAAAGC AGGGCTTTGG GAATCTGCCC ATCTGCATGG CCAAGACACA CTTGTCCTTG 
TCTCACAACC CAGAGCTAAA AGGTGTGCCC ACTGGCTTCG TCCTGCCCAT TCGCGACATC 
CGCGCCAGCG TGGGGGCCGG CTTTCTGTAC CCTCTGGTGG GAACGATGAG CACCATGCCT 
GGACTCCCTA CTCGGCCCTG TTTCTATGAT ATTGATTTGG ACCCTGAAAC TGAGCAGGTG 
AATGGGCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_024997662.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853430.1 Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23501
Clone ID Related Accession (Same CDS sequence)	XM_005211997.2 , XM_024997662.1
Accession Version	XM_024997662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC AAATAAAGGA GAGACTGAAG AACCAAGTCA TTCAGATGAA GGAGGAAGTA 
CCTGGTTTCA TACCAGGCCT GGCAATTTTA CAGGTTGGCA ACAGAGATGA CTCTAATCTT 
TATATAAATA TGAAGTTGAA GGCTGCTGAG GAGATCGGGA TCAAAGCCAC GCACATTAAG 
TTACCCCGGA CGGCCACGGA ATCTGAGGTG TTAAAGTGCA TCACATCATT GAACGAAGAC 
CTCACGGTGC ACGGCTTCAT AGTGCAGCTG CCTTTAGATT CAGAGAATCC CATTAACACT 
GAAACGTTGG TCAATGCTAT CGCCCCCGAA AAAGATGTGG ATGGATTGAA TAGCATCAGT 
GCTGGAAAAC TTGCTAGAGG GGACCTTAGT AACTGTTTCA TCCCTTGTAC ACCCAAAGGA 
TGCTTGGAAC TCATCAAGGA GACAGGGGTG CAAATTGCTG GAAGGCACGC TGTGGTGGTC 
GGGCGCAGTA AAATCGTTGG TGCCCCAATG CATGACTTGC TTCTATGGAA CCATGCCACA 
GTGACCACCT GCCACTCCAA GACTGCCAAT CTGAAAGAGG AGATAAGTAA AGGTGACATC 
CTGGTGGTTG CAGCTGGCCA ACCTGAAATG GTGAAAGGGG AGTGGATCAA ACCTGGGGCG 
ATAGTCATCG ACTGTGGAAT CAATTACGTC ACAGATGATA CAAAACCAAA TGGGAAGAAA 
ATTGTGGGTG ATGTAGCATA CACGGAAGCC AAGGAGAGGG CAAGCTTCAT CACCCCCGTT 
CCTGGTGGCG TCGGGCCCAT GACAGTGGCG ATGCTAATGC AGAGCACAGT AGAGAGTGCA 
AAGCGTTTCT TGGAGAACTT CAAGCCAGGA AAGTGGATCA TTCAGTATAA CAAACTTAAC 
CTGAAGACAC CCGTGCCAAG TGACATTGAT ATATCACGGT CTTACAAGCC AAAGCCCATT 
GGTAACCTGG CTCGAGAAGT TGGTCTGCGC ACTGAAGAGG TAGAATTGTA TGGTGAAACC 
AAGGCTAAAG TTCTGCTGTC GGCGCTGGAA CGGCTGAAGC ACCAGCCTGA TGGGAAATAC 
GTGGTGGTAA CTGGAATCAC TCCAACACCC CTGGGAGAGG GGAAAAGCAC AACCACGATT 
GGGTTGGTGC AGGCCCTTGG CACCCATCTC CATCAGAATG TCTTTGCATG TGTGCGACAG 
CCTTCTCAGG GGCCCACCTT TGGGATAAAA GGTGGCGCTG CAGGCGGTGG CTACTCTCAG 
GTCATTCCTA TGGAAGAGTT TAATCTCCAC CTCACTGGTG ACATCCATGC CATCACTGCA 
GCTAATAACC TTGTGGCCGC AGCCATCGAT GCCCGTATGT TCCATGAAGA GACCCAGACA 
GACAAGGCTC TTTTTAATCG TTTGGTGCCA TCAGTAAATG GAGTGAGAAA GTTCTCCGAT 
ATCCAAATCC GGAGGTTGCG GAGACTAGGC ATTGAAAAGA CTGACCCTGC CACACTGACA 
GATGAAGAGA TAAACAGATT TGCAAGATTG GACATTGATC CAGAAACTAT CACTTGGCAA 
AGAGTGCTGG ATACCAACGA TAGATTCCTG AGGAAGATCA CAATCGGACA GGCTCCAACG 
GAGAAGGGGC ACTCACGGAC GGCCCAGTTT GATATCTCCG TGGCCAGTGA AATCATGGCT 
GTCCTGGCTC TCACCAGTTC TCTAGAAGAC ATGAGAGAGA GACTGGGCAA AATGGTGGTG 
GCGTCCAGCA AGAAAGGGGA GCCCATCAGT ACTGAGGATC TGGGAGTGAG TGGAGCATTG 
ACGGTCCTTA TGAAGGACGC CATCAAGCCC AATCTCATGC AGACGTTAGA GGGCACTCCA 
GTGTTTGTTC ACGCTGGGCC GTTTGCCAAC ATCGCACACG GGAATTCCTC CATCATTGCA 
GACAGGATTG CACTCAAGCT TGTCGGTCCC GAAGGGTTTG TAGTGACTGA AGCAGGATTC 
GGAGCAGACA TTGGAATGGA AAAGTTCTTT AACATCAAAT GCCGGTATTC TGGTCTCCGC 
CCGCACGTGG TGGTGCTTGT CGCCACTGTC AGGGCTCTGA AAATGCACGG AGGTGGCCCC 
ACGGTTACTG CTGGACTTCC GCTTCCCAAG GCCTACATAG AGGAGGACCT GGAGCTGGTT 
GGAAAAGGCT TCAGTAACTT GAAGAAGCAA ATTGAAAATG CCAGGATGTT TGGCGTGCCA 
GTGGTAGTGG CTGTGAACGC CTTCAAGACA GATACGGAGG CCGAGCTGGA CCTTGTCAGC 
CGCCTCGCCA AAGAACATGG TGCTTTTGAC GCCGTGAAGT GCACTCACTG GGCAGAAGGG 
GGCAAGGGCG CCCTGGCGCT GGCTGAGGCC GTCCAGAGGG CAGCTGCGGC CCCCAGCAGC 
TTCCAGCTTC TCTATGACCT CAAGCTCCCA GTTGAGGACA AAATCAGAAT TATTGCGCAG 
AAGATCTATG GAGCTGACAA CATCGAATTG CTCCCTGAAG CCCAGCACAA AGCAGAGGTC 
TACACAAAGC AGGGCTTTGG GAATCTGCCC ATCTGCATGG CCAAGACACA CTTGTCCTTG 
TCTCACAACC CAGAGCTAAA AGGTGTGCCC ACTGGCTTCG TCCTGCCCAT TCGCGACATC 
CGCGCCAGCG TGGGGGCCGG CTTTCTGTAC CCTCTGGTGG GAACGATGAG CACCATGCCT 
GGACTCCCTA CTCGGCCCTG TTTCTATGAT ATTGATTTGG ACCCTGAAAC TGAGCAGGTG 
AATGGGCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853430.1
CDS	204..2978
Translation	MERQIKERLKNQVIQMKEEVPGFIPGLAILQVGNRDDSNLYINMKLKAAEEIGIKATHIKLPRTATESEVLKCITSLNEDLTVHGFIVQLPLDSENPINTETLVNAIAPEKDVDGLNSISAGKLARGDLSNCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINYVTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKPGKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSALERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPATLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHSRTAQFDISVASEIMAVLALTSSLEDMRERLGKMVVASSKKGEPISTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEEDLELVGKGFSNLKKQIENARMFGVPVVVAVNAFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALAEAVQRAAAAPSSFQLLYDLKLPVEDKIRIIAQKIYGADNIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_024997662.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997662.1

ORF Insert Sequence:

ATGGAACGGC AAATAAAGGA GAGACTGAAG AACCAAGTCA TTCAGATGAA GGAGGAAGTA 
CCTGGTTTCA TACCAGGCCT GGCAATTTTA CAGGTTGGCA ACAGAGATGA CTCTAATCTT 
TATATAAATA TGAAGTTGAA GGCTGCTGAG GAGATCGGGA TCAAAGCCAC GCACATTAAG 
TTACCCCGGA CGGCCACGGA ATCTGAGGTG TTAAAGTGCA TCACATCATT GAACGAAGAC 
CTCACGGTGC ACGGCTTCAT AGTGCAGCTG CCTTTAGATT CAGAGAATCC CATTAACACT 
GAAACGTTGG TCAATGCTAT CGCCCCCGAA AAAGATGTGG ATGGATTGAA TAGCATCAGT 
GCTGGAAAAC TTGCTAGAGG GGACCTTAGT AACTGTTTCA TCCCTTGTAC ACCCAAAGGA 
TGCTTGGAAC TCATCAAGGA GACAGGGGTG CAAATTGCTG GAAGGCACGC TGTGGTGGTC 
GGGCGCAGTA AAATCGTTGG TGCCCCAATG CATGACTTGC TTCTATGGAA CCATGCCACA 
GTGACCACCT GCCACTCCAA GACTGCCAAT CTGAAAGAGG AGATAAGTAA AGGTGACATC 
CTGGTGGTTG CAGCTGGCCA ACCTGAAATG GTGAAAGGGG AGTGGATCAA ACCTGGGGCG 
ATAGTCATCG ACTGTGGAAT CAATTACGTC ACAGATGATA CAAAACCAAA TGGGAAGAAA 
ATTGTGGGTG ATGTAGCATA CACGGAAGCC AAGGAGAGGG CAAGCTTCAT CACCCCCGTT 
CCTGGTGGCG TCGGGCCCAT GACAGTGGCG ATGCTAATGC AGAGCACAGT AGAGAGTGCA 
AAGCGTTTCT TGGAGAACTT CAAGCCAGGA AAGTGGATCA TTCAGTATAA CAAACTTAAC 
CTGAAGACAC CCGTGCCAAG TGACATTGAT ATATCACGGT CTTACAAGCC AAAGCCCATT 
GGTAACCTGG CTCGAGAAGT TGGTCTGCGC ACTGAAGAGG TAGAATTGTA TGGTGAAACC 
AAGGCTAAAG TTCTGCTGTC GGCGCTGGAA CGGCTGAAGC ACCAGCCTGA TGGGAAATAC 
GTGGTGGTAA CTGGAATCAC TCCAACACCC CTGGGAGAGG GGAAAAGCAC AACCACGATT 
GGGTTGGTGC AGGCCCTTGG CACCCATCTC CATCAGAATG TCTTTGCATG TGTGCGACAG 
CCTTCTCAGG GGCCCACCTT TGGGATAAAA GGTGGCGCTG CAGGCGGTGG CTACTCTCAG 
GTCATTCCTA TGGAAGAGTT TAATCTCCAC CTCACTGGTG ACATCCATGC CATCACTGCA 
GCTAATAACC TTGTGGCCGC AGCCATCGAT GCCCGTATGT TCCATGAAGA GACCCAGACA 
GACAAGGCTC TTTTTAATCG TTTGGTGCCA TCAGTAAATG GAGTGAGAAA GTTCTCCGAT 
ATCCAAATCC GGAGGTTGCG GAGACTAGGC ATTGAAAAGA CTGACCCTGC CACACTGACA 
GATGAAGAGA TAAACAGATT TGCAAGATTG GACATTGATC CAGAAACTAT CACTTGGCAA 
AGAGTGCTGG ATACCAACGA TAGATTCCTG AGGAAGATCA CAATCGGACA GGCTCCAACG 
GAGAAGGGGC ACTCACGGAC GGCCCAGTTT GATATCTCCG TGGCCAGTGA AATCATGGCT 
GTCCTGGCTC TCACCAGTTC TCTAGAAGAC ATGAGAGAGA GACTGGGCAA AATGGTGGTG 
GCGTCCAGCA AGAAAGGGGA GCCCATCAGT ACTGAGGATC TGGGAGTGAG TGGAGCATTG 
ACGGTCCTTA TGAAGGACGC CATCAAGCCC AATCTCATGC AGACGTTAGA GGGCACTCCA 
GTGTTTGTTC ACGCTGGGCC GTTTGCCAAC ATCGCACACG GGAATTCCTC CATCATTGCA 
GACAGGATTG CACTCAAGCT TGTCGGTCCC GAAGGGTTTG TAGTGACTGA AGCAGGATTC 
GGAGCAGACA TTGGAATGGA AAAGTTCTTT AACATCAAAT GCCGGTATTC TGGTCTCCGC 
CCGCACGTGG TGGTGCTTGT CGCCACTGTC AGGGCTCTGA AAATGCACGG AGGTGGCCCC 
ACGGTTACTG CTGGACTTCC GCTTCCCAAG GCCTACATAG AGGAGGACCT GGAGCTGGTT 
GGAAAAGGCT TCAGTAACTT GAAGAAGCAA ATTGAAAATG CCAGGATGTT TGGCGTGCCA 
GTGGTAGTGG CTGTGAACGC CTTCAAGACA GATACGGAGG CCGAGCTGGA CCTTGTCAGC 
CGCCTCGCCA AAGAACATGG TGCTTTTGAC GCCGTGAAGT GCACTCACTG GGCAGAAGGG 
GGCAAGGGCG CCCTGGCGCT GGCTGAGGCC GTCCAGAGGG CAGCTGCGGC CCCCAGCAGC 
TTCCAGCTTC TCTATGACCT CAAGCTCCCA GTTGAGGACA AAATCAGAAT TATTGCGCAG 
AAGATCTATG GAGCTGACAA CATCGAATTG CTCCCTGAAG CCCAGCACAA AGCAGAGGTC 
TACACAAAGC AGGGCTTTGG GAATCTGCCC ATCTGCATGG CCAAGACACA CTTGTCCTTG 
TCTCACAACC CAGAGCTAAA AGGTGTGCCC ACTGGCTTCG TCCTGCCCAT TCGCGACATC 
CGCGCCAGCG TGGGGGCCGG CTTTCTGTAC CCTCTGGTGG GAACGATGAG CACCATGCCT 
GGACTCCCTA CTCGGCCCTG TTTCTATGAT ATTGATTTGG ACCCTGAAAC TGAGCAGGTG 
AATGGGCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_024997663.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853431.1 Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219636
Clone ID Related Accession (Same CDS sequence)	XM_024997663.1
Accession Version	XM_024997663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGGCTGCTGA GGAGATCGGG ATCAAAGCCA CGCACATTAA GTTACCCCGG 
ACGGCCACGG AATCTGAGGT GTTAAAGTGC ATCACATCAT TGAACGAAGA CCTCACGGTG 
CACGGCTTCA TAGTGCAGCT GCCTTTAGAT TCAGAGAATC CCATTAACAC TGAAACGTTG 
GTCAATGCTA TCGCCCCCGA AAAAGATGTG GATGGATTGA ATAGCATCAG TGCTGGAAAA 
CTTGCTAGAG GGGACCTTAG TAACTGTTTC ATCCCTTGTA CACCCAAAGG ATGCTTGGAA 
CTCATCAAGG AGACAGGGGT GCAAATTGCT GGAAGGCACG CTGTGGTGGT CGGGCGCAGT 
AAAATCGTTG GTGCCCCAAT GCATGACTTG CTTCTATGGA ACCATGCCAC AGTGACCACC 
TGCCACTCCA AGACTGCCAA TCTGAAAGAG GAGATAAGTA AAGGTGACAT CCTGGTGGTT 
GCAGCTGGCC AACCTGAAAT GGTGAAAGGG GAGTGGATCA AACCTGGGGC GATAGTCATC 
GACTGTGGAA TCAATTACGT CACAGATGAT ACAAAACCAA ATGGGAAGAA AATTGTGGGT 
GATGTAGCAT ACACGGAAGC CAAGGAGAGG GCAAGCTTCA TCACCCCCGT TCCTGGTGGC 
GTCGGGCCCA TGACAGTGGC GATGCTAATG CAGAGCACAG TAGAGAGTGC AAAGCGTTTC 
TTGGAGAACT TCAAGCCAGG AAAGTGGATC ATTCAGTATA ACAAACTTAA CCTGAAGACA 
CCCGTGCCAA GTGACATTGA TATATCACGG TCTTACAAGC CAAAGCCCAT TGGTAACCTG 
GCTCGAGAAG TTGGTCTGCG CACTGAAGAG GTAGAATTGT ATGGTGAAAC CAAGGCTAAA 
GTTCTGCTGT CGGCGCTGGA ACGGCTGAAG CACCAGCCTG ATGGGAAATA CGTGGTGGTA 
ACTGGAATCA CTCCAACACC CCTGGGAGAG GGGAAAAGCA CAACCACGAT TGGGTTGGTG 
CAGGCCCTTG GCACCCATCT CCATCAGAAT GTCTTTGCAT GTGTGCGACA GCCTTCTCAG 
GGGCCCACCT TTGGGATAAA AGGTGGCGCT GCAGGCGGTG GCTACTCTCA GGTCATTCCT 
ATGGAAGAGT TTAATCTCCA CCTCACTGGT GACATCCATG CCATCACTGC AGCTAATAAC 
CTTGTGGCCG CAGCCATCGA TGCCCGTATG TTCCATGAAG AGACCCAGAC AGACAAGGCT 
CTTTTTAATC GTTTGGTGCC ATCAGTAAAT GGAGTGAGAA AGTTCTCCGA TATCCAAATC 
CGGAGGTTGC GGAGACTAGG CATTGAAAAG ACTGACCCTG CCACACTGAC AGATGAAGAG 
ATAAACAGAT TTGCAAGATT GGACATTGAT CCAGAAACTA TCACTTGGCA AAGAGTGCTG 
GATACCAACG ATAGATTCCT GAGGAAGATC ACAATCGGAC AGGCTCCAAC GGAGAAGGGG 
CACTCACGGA CGGCCCAGTT TGATATCTCC GTGGCCAGTG AAATCATGGC TGTCCTGGCT 
CTCACCAGTT CTCTAGAAGA CATGAGAGAG AGACTGGGCA AAATGGTGGT GGCGTCCAGC 
AAGAAAGGGG AGCCCATCAG TACTGAGGAT CTGGGAGTGA GTGGAGCATT GACGGTCCTT 
ATGAAGGACG CCATCAAGCC CAATCTCATG CAGACGTTAG AGGGCACTCC AGTGTTTGTT 
CACGCTGGGC CGTTTGCCAA CATCGCACAC GGGAATTCCT CCATCATTGC AGACAGGATT 
GCACTCAAGC TTGTCGGTCC CGAAGGGTTT GTAGTGACTG AAGCAGGATT CGGAGCAGAC 
ATTGGAATGG AAAAGTTCTT TAACATCAAA TGCCGGTATT CTGGTCTCCG CCCGCACGTG 
GTGGTGCTTG TCGCCACTGT CAGGGCTCTG AAAATGCACG GAGGTGGCCC CACGGTTACT 
GCTGGACTTC CGCTTCCCAA GGCCTACATA GAGGAGGACC TGGAGCTGGT TGGAAAAGGC 
TTCAGTAACT TGAAGAAGCA AATTGAAAAT GCCAGGATGT TTGGCGTGCC AGTGGTAGTG 
GCTGTGAACG CCTTCAAGAC AGATACGGAG GCCGAGCTGG ACCTTGTCAG CCGCCTCGCC 
AAAGAACATG GTGCTTTTGA CGCCGTGAAG TGCACTCACT GGGCAGAAGG GGGCAAGGGC 
GCCCTGGCGC TGGCTGAGGC CGTCCAGAGG GCAGCTGCGG CCCCCAGCAG CTTCCAGCTT 
CTCTATGACC TCAAGCTCCC AGTTGAGGAC AAAATCAGAA TTATTGCGCA GAAGATCTAT 
GGAGCTGACA ACATCGAATT GCTCCCTGAA GCCCAGCACA AAGCAGAGGT CTACACAAAG 
CAGGGCTTTG GGAATCTGCC CATCTGCATG GCCAAGACAC ACTTGTCCTT GTCTCACAAC 
CCAGAGCTAA AAGGTGTGCC CACTGGCTTC GTCCTGCCCA TTCGCGACAT CCGCGCCAGC 
GTGGGGGCCG GCTTTCTGTA CCCTCTGGTG GGAACGATGA GCACCATGCC TGGACTCCCT 
ACTCGGCCCT GTTTCTATGA TATTGATTTG GACCCTGAAA CTGAGCAGGT GAATGGGCTC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853431.1
CDS	162..2807
Translation	MKLKAAEEIGIKATHIKLPRTATESEVLKCITSLNEDLTVHGFIVQLPLDSENPINTETLVNAIAPEKDVDGLNSISAGKLARGDLSNCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINYVTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKPGKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSALERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPATLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHSRTAQFDISVASEIMAVLALTSSLEDMRERLGKMVVASSKKGEPISTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEEDLELVGKGFSNLKKQIENARMFGVPVVVAVNAFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALAEAVQRAAAAPSSFQLLYDLKLPVEDKIRIIAQKIYGADNIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_024997663.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997663.1

ORF Insert Sequence:

ATGAAGTTGA AGGCTGCTGA GGAGATCGGG ATCAAAGCCA CGCACATTAA GTTACCCCGG 
ACGGCCACGG AATCTGAGGT GTTAAAGTGC ATCACATCAT TGAACGAAGA CCTCACGGTG 
CACGGCTTCA TAGTGCAGCT GCCTTTAGAT TCAGAGAATC CCATTAACAC TGAAACGTTG 
GTCAATGCTA TCGCCCCCGA AAAAGATGTG GATGGATTGA ATAGCATCAG TGCTGGAAAA 
CTTGCTAGAG GGGACCTTAG TAACTGTTTC ATCCCTTGTA CACCCAAAGG ATGCTTGGAA 
CTCATCAAGG AGACAGGGGT GCAAATTGCT GGAAGGCACG CTGTGGTGGT CGGGCGCAGT 
AAAATCGTTG GTGCCCCAAT GCATGACTTG CTTCTATGGA ACCATGCCAC AGTGACCACC 
TGCCACTCCA AGACTGCCAA TCTGAAAGAG GAGATAAGTA AAGGTGACAT CCTGGTGGTT 
GCAGCTGGCC AACCTGAAAT GGTGAAAGGG GAGTGGATCA AACCTGGGGC GATAGTCATC 
GACTGTGGAA TCAATTACGT CACAGATGAT ACAAAACCAA ATGGGAAGAA AATTGTGGGT 
GATGTAGCAT ACACGGAAGC CAAGGAGAGG GCAAGCTTCA TCACCCCCGT TCCTGGTGGC 
GTCGGGCCCA TGACAGTGGC GATGCTAATG CAGAGCACAG TAGAGAGTGC AAAGCGTTTC 
TTGGAGAACT TCAAGCCAGG AAAGTGGATC ATTCAGTATA ACAAACTTAA CCTGAAGACA 
CCCGTGCCAA GTGACATTGA TATATCACGG TCTTACAAGC CAAAGCCCAT TGGTAACCTG 
GCTCGAGAAG TTGGTCTGCG CACTGAAGAG GTAGAATTGT ATGGTGAAAC CAAGGCTAAA 
GTTCTGCTGT CGGCGCTGGA ACGGCTGAAG CACCAGCCTG ATGGGAAATA CGTGGTGGTA 
ACTGGAATCA CTCCAACACC CCTGGGAGAG GGGAAAAGCA CAACCACGAT TGGGTTGGTG 
CAGGCCCTTG GCACCCATCT CCATCAGAAT GTCTTTGCAT GTGTGCGACA GCCTTCTCAG 
GGGCCCACCT TTGGGATAAA AGGTGGCGCT GCAGGCGGTG GCTACTCTCA GGTCATTCCT 
ATGGAAGAGT TTAATCTCCA CCTCACTGGT GACATCCATG CCATCACTGC AGCTAATAAC 
CTTGTGGCCG CAGCCATCGA TGCCCGTATG TTCCATGAAG AGACCCAGAC AGACAAGGCT 
CTTTTTAATC GTTTGGTGCC ATCAGTAAAT GGAGTGAGAA AGTTCTCCGA TATCCAAATC 
CGGAGGTTGC GGAGACTAGG CATTGAAAAG ACTGACCCTG CCACACTGAC AGATGAAGAG 
ATAAACAGAT TTGCAAGATT GGACATTGAT CCAGAAACTA TCACTTGGCA AAGAGTGCTG 
GATACCAACG ATAGATTCCT GAGGAAGATC ACAATCGGAC AGGCTCCAAC GGAGAAGGGG 
CACTCACGGA CGGCCCAGTT TGATATCTCC GTGGCCAGTG AAATCATGGC TGTCCTGGCT 
CTCACCAGTT CTCTAGAAGA CATGAGAGAG AGACTGGGCA AAATGGTGGT GGCGTCCAGC 
AAGAAAGGGG AGCCCATCAG TACTGAGGAT CTGGGAGTGA GTGGAGCATT GACGGTCCTT 
ATGAAGGACG CCATCAAGCC CAATCTCATG CAGACGTTAG AGGGCACTCC AGTGTTTGTT 
CACGCTGGGC CGTTTGCCAA CATCGCACAC GGGAATTCCT CCATCATTGC AGACAGGATT 
GCACTCAAGC TTGTCGGTCC CGAAGGGTTT GTAGTGACTG AAGCAGGATT CGGAGCAGAC 
ATTGGAATGG AAAAGTTCTT TAACATCAAA TGCCGGTATT CTGGTCTCCG CCCGCACGTG 
GTGGTGCTTG TCGCCACTGT CAGGGCTCTG AAAATGCACG GAGGTGGCCC CACGGTTACT 
GCTGGACTTC CGCTTCCCAA GGCCTACATA GAGGAGGACC TGGAGCTGGT TGGAAAAGGC 
TTCAGTAACT TGAAGAAGCA AATTGAAAAT GCCAGGATGT TTGGCGTGCC AGTGGTAGTG 
GCTGTGAACG CCTTCAAGAC AGATACGGAG GCCGAGCTGG ACCTTGTCAG CCGCCTCGCC 
AAAGAACATG GTGCTTTTGA CGCCGTGAAG TGCACTCACT GGGCAGAAGG GGGCAAGGGC 
GCCCTGGCGC TGGCTGAGGC CGTCCAGAGG GCAGCTGCGG CCCCCAGCAG CTTCCAGCTT 
CTCTATGACC TCAAGCTCCC AGTTGAGGAC AAAATCAGAA TTATTGCGCA GAAGATCTAT 
GGAGCTGACA ACATCGAATT GCTCCCTGAA GCCCAGCACA AAGCAGAGGT CTACACAAAG 
CAGGGCTTTG GGAATCTGCC CATCTGCATG GCCAAGACAC ACTTGTCCTT GTCTCACAAC 
CCAGAGCTAA AAGGTGTGCC CACTGGCTTC GTCCTGCCCA TTCGCGACAT CCGCGCCAGC 
GTGGGGGCCG GCTTTCTGTA CCCTCTGGTG GGAACGATGA GCACCATGCC TGGACTCCCT 
ACTCGGCCCT GTTTCTATGA TATTGATTTG GACCCTGAAA CTGAGCAGGT GAATGGGCTC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( XM_024997664.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853432.1 Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X3, mRNA.

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CloneID	OBa219637
Clone ID Related Accession (Same CDS sequence)	XM_024997664.1
Accession Version	XM_024997664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGACT TGCTTCTATG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGAAAG AGGAGATAAG TAAAGGTGAC ATCCTGGTGG TTGCAGCTGG CCAACCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCGATAGTCA TCGACTGTGG AATCAATTAC 
GTCACAGATG ATACAAAACC AAATGGGAAG AAAATTGTGG GTGATGTAGC ATACACGGAA 
GCCAAGGAGA GGGCAAGCTT CATCACCCCC GTTCCTGGTG GCGTCGGGCC CATGACAGTG 
GCGATGCTAA TGCAGAGCAC AGTAGAGAGT GCAAAGCGTT TCTTGGAGAA CTTCAAGCCA 
GGAAAGTGGA TCATTCAGTA TAACAAACTT AACCTGAAGA CACCCGTGCC AAGTGACATT 
GATATATCAC GGTCTTACAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AGTTGGTCTG 
CGCACTGAAG AGGTAGAATT GTATGGTGAA ACCAAGGCTA AAGTTCTGCT GTCGGCGCTG 
GAACGGCTGA AGCACCAGCC TGATGGGAAA TACGTGGTGG TAACTGGAAT CACTCCAACA 
CCCCTGGGAG AGGGGAAAAG CACAACCACG ATTGGGTTGG TGCAGGCCCT TGGCACCCAT 
CTCCATCAGA ATGTCTTTGC ATGTGTGCGA CAGCCTTCTC AGGGGCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGCGG TGGCTACTCT CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTGGC CGCAGCCATC 
GATGCCCGTA TGTTCCATGA AGAGACCCAG ACAGACAAGG CTCTTTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCC GATATCCAAA TCCGGAGGTT GCGGAGACTA 
GGCATTGAAA AGACTGACCC TGCCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC TATCACTTGG CAAAGAGTGC TGGATACCAA CGATAGATTC 
CTGAGGAAGA TCACAATCGG ACAGGCTCCA ACGGAGAAGG GGCACTCACG GACGGCCCAG 
TTTGATATCT CCGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCGTCCA GCAAGAAAGG GGAGCCCATC 
AGTACTGAGG ATCTGGGAGT GAGTGGAGCA TTGACGGTCC TTATGAAGGA CGCCATCAAG 
CCCAATCTCA TGCAGACGTT AGAGGGCACT CCAGTGTTTG TTCACGCTGG GCCGTTTGCC 
AACATCGCAC ACGGGAATTC CTCCATCATT GCAGACAGGA TTGCACTCAA GCTTGTCGGT 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCAG ACATTGGAAT GGAAAAGTTC 
TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCGCACG TGGTGGTGCT TGTCGCCACT 
GTCAGGGCTC TGAAAATGCA CGGAGGTGGC CCCACGGTTA CTGCTGGACT TCCGCTTCCC 
AAGGCCTACA TAGAGGAGGA CCTGGAGCTG GTTGGAAAAG GCTTCAGTAA CTTGAAGAAG 
CAAATTGAAA ATGCCAGGAT GTTTGGCGTG CCAGTGGTAG TGGCTGTGAA CGCCTTCAAG 
ACAGATACGG AGGCCGAGCT GGACCTTGTC AGCCGCCTCG CCAAAGAACA TGGTGCTTTT 
GACGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GCGCCCTGGC GCTGGCTGAG 
GCCGTCCAGA GGGCAGCTGC GGCCCCCAGC AGCTTCCAGC TTCTCTATGA CCTCAAGCTC 
CCAGTTGAGG ACAAAATCAG AATTATTGCG CAGAAGATCT ATGGAGCTGA CAACATCGAA 
TTGCTCCCTG AAGCCCAGCA CAAAGCAGAG GTCTACACAA AGCAGGGCTT TGGGAATCTG 
CCCATCTGCA TGGCCAAGAC ACACTTGTCC TTGTCTCACA ACCCAGAGCT AAAAGGTGTG 
CCCACTGGCT TCGTCCTGCC CATTCGCGAC ATCCGCGCCA GCGTGGGGGC CGGCTTTCTG 
TACCCTCTGG TGGGAACGAT GAGCACCATG CCTGGACTCC CTACTCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACTGAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853432.1
CDS	200..2467
Translation	MHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINYVTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKPGKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSALERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPATLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHSRTAQFDISVASEIMAVLALTSSLEDMRERLGKMVVASSKKGEPISTEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEEDLELVGKGFSNLKKQIENARMFGVPVVVAVNAFKTDTEAELDLVSRLAKEHGAFDAVKCTHWAEGGKGALALAEAVQRAAAAPSSFQLLYDLKLPVEDKIRIIAQKIYGADNIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_024997664.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997664.1

ORF Insert Sequence:

ATGCATGACT TGCTTCTATG GAACCATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGAAAG AGGAGATAAG TAAAGGTGAC ATCCTGGTGG TTGCAGCTGG CCAACCTGAA 
ATGGTGAAAG GGGAGTGGAT CAAACCTGGG GCGATAGTCA TCGACTGTGG AATCAATTAC 
GTCACAGATG ATACAAAACC AAATGGGAAG AAAATTGTGG GTGATGTAGC ATACACGGAA 
GCCAAGGAGA GGGCAAGCTT CATCACCCCC GTTCCTGGTG GCGTCGGGCC CATGACAGTG 
GCGATGCTAA TGCAGAGCAC AGTAGAGAGT GCAAAGCGTT TCTTGGAGAA CTTCAAGCCA 
GGAAAGTGGA TCATTCAGTA TAACAAACTT AACCTGAAGA CACCCGTGCC AAGTGACATT 
GATATATCAC GGTCTTACAA GCCAAAGCCC ATTGGTAACC TGGCTCGAGA AGTTGGTCTG 
CGCACTGAAG AGGTAGAATT GTATGGTGAA ACCAAGGCTA AAGTTCTGCT GTCGGCGCTG 
GAACGGCTGA AGCACCAGCC TGATGGGAAA TACGTGGTGG TAACTGGAAT CACTCCAACA 
CCCCTGGGAG AGGGGAAAAG CACAACCACG ATTGGGTTGG TGCAGGCCCT TGGCACCCAT 
CTCCATCAGA ATGTCTTTGC ATGTGTGCGA CAGCCTTCTC AGGGGCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGCGG TGGCTACTCT CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTTGTGGC CGCAGCCATC 
GATGCCCGTA TGTTCCATGA AGAGACCCAG ACAGACAAGG CTCTTTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAAGTTCTCC GATATCCAAA TCCGGAGGTT GCGGAGACTA 
GGCATTGAAA AGACTGACCC TGCCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC TATCACTTGG CAAAGAGTGC TGGATACCAA CGATAGATTC 
CTGAGGAAGA TCACAATCGG ACAGGCTCCA ACGGAGAAGG GGCACTCACG GACGGCCCAG 
TTTGATATCT CCGTGGCCAG TGAAATCATG GCTGTCCTGG CTCTCACCAG TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCGTCCA GCAAGAAAGG GGAGCCCATC 
AGTACTGAGG ATCTGGGAGT GAGTGGAGCA TTGACGGTCC TTATGAAGGA CGCCATCAAG 
CCCAATCTCA TGCAGACGTT AGAGGGCACT CCAGTGTTTG TTCACGCTGG GCCGTTTGCC 
AACATCGCAC ACGGGAATTC CTCCATCATT GCAGACAGGA TTGCACTCAA GCTTGTCGGT 
CCCGAAGGGT TTGTAGTGAC TGAAGCAGGA TTCGGAGCAG ACATTGGAAT GGAAAAGTTC 
TTTAACATCA AATGCCGGTA TTCTGGTCTC CGCCCGCACG TGGTGGTGCT TGTCGCCACT 
GTCAGGGCTC TGAAAATGCA CGGAGGTGGC CCCACGGTTA CTGCTGGACT TCCGCTTCCC 
AAGGCCTACA TAGAGGAGGA CCTGGAGCTG GTTGGAAAAG GCTTCAGTAA CTTGAAGAAG 
CAAATTGAAA ATGCCAGGAT GTTTGGCGTG CCAGTGGTAG TGGCTGTGAA CGCCTTCAAG 
ACAGATACGG AGGCCGAGCT GGACCTTGTC AGCCGCCTCG CCAAAGAACA TGGTGCTTTT 
GACGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GCGCCCTGGC GCTGGCTGAG 
GCCGTCCAGA GGGCAGCTGC GGCCCCCAGC AGCTTCCAGC TTCTCTATGA CCTCAAGCTC 
CCAGTTGAGG ACAAAATCAG AATTATTGCG CAGAAGATCT ATGGAGCTGA CAACATCGAA 
TTGCTCCCTG AAGCCCAGCA CAAAGCAGAG GTCTACACAA AGCAGGGCTT TGGGAATCTG 
CCCATCTGCA TGGCCAAGAC ACACTTGTCC TTGTCTCACA ACCCAGAGCT AAAAGGTGTG 
CCCACTGGCT TCGTCCTGCC CATTCGCGAC ATCCGCGCCA GCGTGGGGGC CGGCTTTCTG 
TACCCTCTGG TGGGAACGAT GAGCACCATG CCTGGACTCC CTACTCGGCC CTGTTTCTAT 
GATATTGATT TGGACCCTGA AACTGAGCAG GTGAATGGGC TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MTHFD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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