Ehmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ehmt1
Entrez Gene ID	77683
Full Name	euchromatic histone methyltransferase 1
Synonyms	9230102N17Rik,D330003E03,Eu-HMTase1,GLP,GLP1,KMT1D,mKIAA1876
General protein information	Preferred Names euchromatic histone methyltransferase 1 Names histone-lysine N-methyltransferase EHMT1 G9a-like protein 1 euchromatic histone-lysine N-methyltransferase 1 lysine N-methyltransferase 1D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006498422.2	XP_006498485.1	histone-lysine N-methyltransferase EHMT1 isoform X1
XM_030252214.1	XP_030108074.1	histone-lysine N-methyltransferase EHMT1 isoform X15
XM_030252216.1	XP_030108076.1	histone-lysine N-methyltransferase EHMT1 isoform X18
XM_030252221.1	XP_030108081.1	histone-lysine N-methyltransferase EHMT1 isoform X23
XM_006498429.2	XP_006498492.1	histone-lysine N-methyltransferase EHMT1 isoform X7
XM_006498425.2	XP_006498488.1	histone-lysine N-methyltransferase EHMT1 isoform X4
XM_006498430.2	XP_006498493.1	histone-lysine N-methyltransferase EHMT1 isoform X13
XM_030252218.1	XP_030108078.1	histone-lysine N-methyltransferase EHMT1 isoform X20
XM_030252219.1	XP_030108079.1	histone-lysine N-methyltransferase EHMT1 isoform X21
XM_030252220.1	XP_030108080.1	histone-lysine N-methyltransferase EHMT1 isoform X22
XM_030252231.1	XP_030108091.1	histone-lysine N-methyltransferase EHMT1 isoform X34
XM_006498427.2	XP_006498490.1	histone-lysine N-methyltransferase EHMT1 isoform X6
XM_017319335.1	XP_017174824.1	histone-lysine N-methyltransferase EHMT1 isoform X10
XM_030252224.1	XP_030108084.1	histone-lysine N-methyltransferase EHMT1 isoform X27
XM_030252230.1	XP_030108090.1	histone-lysine N-methyltransferase EHMT1 isoform X33
NM_001109686.2	NP_001103156.1	histone-lysine N-methyltransferase EHMT1 isoform 3
NM_001109687.2	NP_001103157.1	histone-lysine N-methyltransferase EHMT1 isoform 4
NM_001012518.3	NP_001012536.2	histone-lysine N-methyltransferase EHMT1 isoform 1
XM_006498423.2	XP_006498486.1	histone-lysine N-methyltransferase EHMT1 isoform X2
XM_030252211.1	XP_030108071.1	histone-lysine N-methyltransferase EHMT1 isoform X11
XM_030252212.1	XP_030108072.1	histone-lysine N-methyltransferase EHMT1 isoform X12
XM_006498432.2	XP_006498495.1	histone-lysine N-methyltransferase EHMT1 isoform X17
XM_030252217.1	XP_030108077.1	histone-lysine N-methyltransferase EHMT1 isoform X19
XM_030252222.1	XP_030108082.1	histone-lysine N-methyltransferase EHMT1 isoform X24
XM_030252223.1	XP_030108083.1	histone-lysine N-methyltransferase EHMT1 isoform X25
XM_030252225.1	XP_030108085.1	histone-lysine N-methyltransferase EHMT1 isoform X28
XM_030252228.1	XP_030108088.1	histone-lysine N-methyltransferase EHMT1 isoform X31
XM_006498424.2	XP_006498487.1	histone-lysine N-methyltransferase EHMT1 isoform X3
XM_030252207.1	XP_030108067.1	histone-lysine N-methyltransferase EHMT1 isoform X5
XM_006498426.2	XP_006498489.1	histone-lysine N-methyltransferase EHMT1 isoform X6
XM_030252208.1	XP_030108068.1	histone-lysine N-methyltransferase EHMT1 isoform X7
XM_030252209.1	XP_030108069.1	histone-lysine N-methyltransferase EHMT1 isoform X8
XM_030252210.1	XP_030108070.1	histone-lysine N-methyltransferase EHMT1 isoform X9
XM_030252213.1	XP_030108073.1	histone-lysine N-methyltransferase EHMT1 isoform X12
XM_006498431.2	XP_006498494.1	histone-lysine N-methyltransferase EHMT1 isoform X14
XM_030252215.1	XP_030108075.1	histone-lysine N-methyltransferase EHMT1 isoform X16
XM_017319336.1	XP_017174825.1	histone-lysine N-methyltransferase EHMT1 isoform X26
XM_030252226.1	XP_030108086.1	histone-lysine N-methyltransferase EHMT1 isoform X29
XM_030252227.1	XP_030108087.1	histone-lysine N-methyltransferase EHMT1 isoform X30
XM_030252229.1	XP_030108089.1	histone-lysine N-methyltransferase EHMT1 isoform X32
NM_172545.4	NP_766133.2	histone-lysine N-methyltransferase EHMT1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EHMT1	NP_079033.4
Pan troglodytes (chimpanzee)	EHMT1	XP_520395.3
Bos taurus (cattle)	EHMT1	NP_001092511.1
Mus musculus (house mouse)	Ehmt1	NP_001012536.2
Rattus norvegicus (Norway rat)	Ehmt1	NP_001102042.1
Gallus gallus (chicken)	EHMT1	NP_001012550.1
Xenopus tropicalis (tropical clawed frog)	ehmt1	NP_001096168.1
Danio rerio (zebrafish)	ehmt1b	NP_001166035.1
Canis lupus familiaris (dog)	EHMT1	XP_005625091.1

Gene Ontology
Bibliography

Related articles in PubMed

Repression of Adipose Tissue Fibrosis through a PRDM16-GTF2IRD1 Complex Improves Systemic Glucose Homeostasis.
Hasegawa Y, Ikeda K, Chen Y, Alba DL, Stifler D, Shinoda K, Hosono T, Maretich P, Yang Y, Ishigaki Y, Chi J, Cohen P, Koliwad SK, Kajimura S
Cell metabolism27(1)180-194.e6(2018 Jan)

Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration.
Hatazawa Y, Ono Y, Hirose Y, Kanai S, Fujii NL, Machida S, Nishino I, Shimizu T, Okano M, Kamei Y, Ogawa Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)

GLP inhibits heterochromatin clustering and myogenic differentiation by repressing MeCP2.
Choi MH, Palanichamy Kala M, Ow JR, Rao VK, Suriyamurthy S, Taneja R
Journal of molecular cell biology(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GLP/G9a H3K9 methyltransferase complex is an enzyme counteracting Jmjd1a-mediated H3K9 demethylation at the Sry locus in gonadal somatic cells
Title: Rescuing the aberrant sex development of H3K9 demethylase Jmjd1a-deficient mice by modulating H3K9 methylation balance.

miR-217-mediated, genetic, or pharmacological inactivation of EHMT1/2 was sufficient to promote pathological hypertrophy
Title: The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.

EHMT1 Mediates Homeostatic Synaptic Scaling
Title: Histone Methylation by the Kleefstra Syndrome Protein EHMT1 Mediates Homeostatic Synaptic Scaling.

The stress-induced Brg1-G9a/GLP-Dnmt3 interactions and sequence of repressive chromatin assembly on Myh6 promoter illustrates a molecular mechanism by which the heart epigenetically responds to environmental signals.
Title: Epigenetic response to environmental stress: Assembly of BRG1-G9a/GLP-DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts.

data provide genetic and pharmacologic evidence that EHMT1 and EHMT2 are epigenetic regulators involved in gamma-globin repression and represent a novel therapeutic target for SCD.
Title: EHMT1 and EHMT2 inhibition induces fetal hemoglobin expression.

The following Ehmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ehmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80465	XM_017319335.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80466	XM_017319336.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88044	XM_030252207.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88045	XM_030252208.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88046	XM_030252209.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88047	XM_030252210.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88048	XM_030252211.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88049	XM_030252213.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88049	XM_030252212.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88050	XM_030252214.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88051	XM_030252215.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88052	XM_030252216.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88053	XM_030252217.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88054	XM_030252218.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88055	XM_030252219.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88056	XM_030252220.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88057	XM_030252221.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88058	XM_030252222.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88059	XM_030252223.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88060	XM_030252224.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88061	XM_030252225.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88062	XM_030252226.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88063	XM_030252227.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88064	XM_030252228.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88065	XM_030252229.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88066	XM_030252230.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88067	XM_030252231.1 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu16352	NM_172545.4 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$762.30 $1089.00
OMu21886	NM_001109686.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu21894	NM_001109687.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu23314	NM_001012518.3 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$762.30 $1089.00
OMu29800	XM_006498422.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29801	XM_006498423.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29802	XM_006498424.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29803	XM_006498425.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29804	XM_006498426.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29805	XM_006498427.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29807	XM_006498429.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29808	XM_006498430.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29809	XM_006498431.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29810	XM_006498432.2 Latest version!	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ehmt1 ( XM_017319335.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174824.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X10, mRNA.

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CloneID	OMu80465
Clone ID Related Accession (Same CDS sequence)	XM_017319335.1
Accession Version	XM_017319335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGGTAAAGA TACACCTATG GCTGCTGATG AAGGTTCCAC AGAGAAACAA 
GAAGGAGAGA CTCCCATGGC TGCAGATGGA GAAACAAATG GGTCTTGTGA AAAGAGTGGG 
GATCCCAGCC ATCTAAATGC ACCCAAACAC ACTCAGGAGA ACACAAGAGC TAGCCCACAG 
GAAGGCACCA ACAGAGTGTC TCGGGTGGCA GAAAATGGGG TTTCAGAAAG AGACACAGAA 
GTGGGGAAGC AAAACCATGT CACAGCTGAC GACTTCATGC AGACATCTGT CATTGGCAGC 
AATGGATATT TCTTAAATAA ACCAGCCCTG CAGGGGCAGC CGTTGAGGAC TCCCAACATT 
CTAACCTCCT CGCTTCCTGG TCATGCTGCA AAAACTCTTC CTGGAGGAGC CAGTAAATGC 
AGGACTCTGA GTGCACTTCC TCAGACACCA ACCACAGCAC CCACTGTGCC TGGGGAAGGG 
AGTGCAGACA CAGAGGACAG AAAGCCTACA GCCTCGGGCA CTGATGTCAG GGTTCACAGG 
GCACGCAAGA CCATGCCGAA GTCCATCTTG GGCCTGCATG CAGCCAGCAA AGACCATAGA 
GAAGTTCAAG ACCATAAGGA ACCAAAAGAG GATATCAACA GAAACATTTC TGAATGTGGA 
CGACAGCAGC TTTTACCAAC CTTCCCAGCC CTCCACCAGT CGCTACCTCA GAATCAGTGC 
TACATGGCCA CCACAAAGTC CCAGACAGCA GCTGCAGTAT CTCGGAAGAA AAAACGAAGA 
ATGGGAACCT ATAGTTTAGT TCCCAAGAAA AAGACCAAAG TATTAAAACA GAGGACGGTG 
ATTGAGATGT TTAAAAGCAT AACCCATTCC ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA 
GATGATAGTG CCCTGCATGT AAATGGCGAG AGCTTGGAGA TGGACTCAGA AGATGAAGAC 
TCCGATGAGC TGGAGGATGA CGAGGACCAT GGAGCTGAGC AAGCGGCTGC ATTTCCCACC 
GAGGATAGTA GGACTTCTAA AGAGAGCATG TCTGAGACTG ACCGGGCTGC AAAGATGGAT 
GGAGATTCAG AGGAGGAACA GGAGTCTCCC GACACAGGGG AGGATGAAGA TGGTGGAGAC 
GAGTCTGACC TGAGTTCTGA ATCCAGTATC AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG 
ACTGACAGCC CCTGGATCAA ACCTGCTCGG AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG 
AGCAGCATGC TTGGTTCCGA GGCTTGTAAG TCATCTCCAG GAAGCATGGA GCAGGCAGCT 
CTGGGAGACA GCGCTGGCTA TATGGAAGTT TCCCTGGATT CCCTGGATCT CCGTGTCAGA 
GGAATTCTGT CTTCCCAGAC AGAAAATGAA GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAATTG 
GGCCGGTGTA CAAACAGCGT GGTCAAGTAT GAGCTGATGC GCCCATCCAA CAAAGCACCG 
CTCTTGGTGT TATGTGAAGA CCATCGGGGT CGCATGGTGA AGCACCAGTG CTGTCCTGGC 
TGCGGCTACT TCTGCACGGC GGGTAACTTC ATGGAATGCC AGCCTGAGAG CAGCATCTCT 
CATCGTTTCC ATAAGGACTG TGCCTCTCGA GTCAACAATG CCAGCTACTG TCCCCATTGT 
GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG ACCATAGCAA AAGCAGACAC AACTTCCACA 
GTGACCCTAG CCCCTGGACA GGAGAAGAGC CTGGCTGCTG AAGGCAGGGC TGACACGACC 
ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT GAAAGATCGC AGAGCACAGC CCCCCAGGCA 
CCAGAGTGCT TCGACCCTGC CGGACCGGCT GGGCTCGTGA GGCCGACATC TGGCCTTTCC 
CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT GCTCTAATCG CTCTAGACTC TGAAAAACCC 
AAGAAACTTC GCTTCCACCC AAAGCAGCTG TACTTCTCTG CCAGGCAGGG TGAGCTGCAG 
AAGGTGCTTC TCATGCTGGT TGATGGAATT GATCCCAACT TCAAAATGGA GCACCAAAGT 
AAGCGTTCCC CATTACATGC TGCTGCGGAG GCTGGCCACG TGGACATCTG CCACATGCTG 
GTTCAGGCGG GTGCCAATAT TGACACTTGC TCAGAGGACC AGCGGACCCC ACTGATGGAG 
GCTGCAGAGA ACAACCACTT GGATGCAGTG AAGTACCTCA TCAAGGCTGG AGCACAGGTG 
GATCCGAAGG ACGCAGAGGG CTCCACATGT TTGCATTTGG CTGCCAAGAA AGGCCACTAT 
GATGTGGTTC AGTATCTGCT TTCAAATGGA CAGATGGATG TCAACTGCCA GGATGACGGT 
GGATGGACAC CTATGATCTG GGCCACTGAG TACAAGCACG TGGAGCTGGT GAAGCTGCTG 
CTGTCTAAGG GCTCTGACAT CAACATCCGG GACAACGAGG AGAACATTTG TCTGCACTGG 
GCAGCATTTT CAGGCTGTGT GGACATAGCT GAAATACTTC TGGCTGCCAA GTGTGACCTG 
CATGCTGTGA ATATCCATGG AGACTCACCC CTGCACATCG CAGCCAGGGA GAATCGCTAC 
GACTGTGTTG TCCTCTTTCT TTCTCGGGAT TCAGATGTTA CTCTGAAAAA CAAGGAAGGA 
GAGACTCCCT TGCAGTGTGC AAGTCTCAGT TCGCAGGTGT GGAGTGCATT GCAGATGAGC 
AAAGCACTTC GGGACTCAGC CCCTGACAAG CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG 
GATATCGCTC GAGGGTATGA GCGCATTCCC ATTCCCTGTG TCAATGCTGT GGACAGTGAG 
CTGTGTCCTA CCAACTATAA GTATGTCTCC CAGAACTGTG TGACATCCCC CATGAACATT 
GACAGGAACA TCACTCATTT GCAGTACTGC GTGTGTGTAG ATGACTGCTC CTCTAGCACC 
TGCATGTGTG GCCAGCTGAG CATGCGCTGC TGGTATGATA AGGATGGCCG ACTTCTGCCT 
GAGTTTAACA TGGCAGAACC ACCCTTGATC TTCGAGTGCA ATCATGCCTG CTCATGCTGG 
AGGAACTGCC GCAATCGTGT GGTGCAAAAT GGTCTCAGGG CAAGGCTGCA GCTTTATCGG 
ACACAGGACA TGGGCTGGGG TGTGCGGTCC CTCCAGGATA TCCCACTGGG CACCTTTGTC 
TGCGAATACG TAGGGGAGCT GATTTCGGAC TCTGAAGCTG ATGTTCGGGA AGAGGACTCT 
TACCTCTTTG ATCTTGACAA TAAGGATGGA GAGGTATACT GCATTGACGC TCGGTTCTAT 
GGGAATGTCA GCCGGTTCAT AAACCACCAC TGCGAACCCA ACCTTGTGCC TGTGCGAGTG 
TTCATGTCAC ACCAGGACCT GCGGTTTCCC AGGATTGCCT TCTTCAGTAC CCGCCTGATT 
CAGGCTGGGG AGCAGCTCGG GTTCGACTAC GGGGAGCGCT TTTGGGACGT CAAGGGCAAG 
CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG TGTCGGCACT CAAGCGCAGC CCTGGCCCAG 
AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG GAGAATGGCC TTCCAGATAC CAGCTCTGCA 
GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174824.1
CDS	172..4032
Translation	MKQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_017319335.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319335.1

ORF Insert Sequence:

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGGTAAAGA TACACCTATG GCTGCTGATG AAGGTTCCAC AGAGAAACAA 
GAAGGAGAGA CTCCCATGGC TGCAGATGGA GAAACAAATG GGTCTTGTGA AAAGAGTGGG 
GATCCCAGCC ATCTAAATGC ACCCAAACAC ACTCAGGAGA ACACAAGAGC TAGCCCACAG 
GAAGGCACCA ACAGAGTGTC TCGGGTGGCA GAAAATGGGG TTTCAGAAAG AGACACAGAA 
GTGGGGAAGC AAAACCATGT CACAGCTGAC GACTTCATGC AGACATCTGT CATTGGCAGC 
AATGGATATT TCTTAAATAA ACCAGCCCTG CAGGGGCAGC CGTTGAGGAC TCCCAACATT 
CTAACCTCCT CGCTTCCTGG TCATGCTGCA AAAACTCTTC CTGGAGGAGC CAGTAAATGC 
AGGACTCTGA GTGCACTTCC TCAGACACCA ACCACAGCAC CCACTGTGCC TGGGGAAGGG 
AGTGCAGACA CAGAGGACAG AAAGCCTACA GCCTCGGGCA CTGATGTCAG GGTTCACAGG 
GCACGCAAGA CCATGCCGAA GTCCATCTTG GGCCTGCATG CAGCCAGCAA AGACCATAGA 
GAAGTTCAAG ACCATAAGGA ACCAAAAGAG GATATCAACA GAAACATTTC TGAATGTGGA 
CGACAGCAGC TTTTACCAAC CTTCCCAGCC CTCCACCAGT CGCTACCTCA GAATCAGTGC 
TACATGGCCA CCACAAAGTC CCAGACAGCA GCTGCAGTAT CTCGGAAGAA AAAACGAAGA 
ATGGGAACCT ATAGTTTAGT TCCCAAGAAA AAGACCAAAG TATTAAAACA GAGGACGGTG 
ATTGAGATGT TTAAAAGCAT AACCCATTCC ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA 
GATGATAGTG CCCTGCATGT AAATGGCGAG AGCTTGGAGA TGGACTCAGA AGATGAAGAC 
TCCGATGAGC TGGAGGATGA CGAGGACCAT GGAGCTGAGC AAGCGGCTGC ATTTCCCACC 
GAGGATAGTA GGACTTCTAA AGAGAGCATG TCTGAGACTG ACCGGGCTGC AAAGATGGAT 
GGAGATTCAG AGGAGGAACA GGAGTCTCCC GACACAGGGG AGGATGAAGA TGGTGGAGAC 
GAGTCTGACC TGAGTTCTGA ATCCAGTATC AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG 
ACTGACAGCC CCTGGATCAA ACCTGCTCGG AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG 
AGCAGCATGC TTGGTTCCGA GGCTTGTAAG TCATCTCCAG GAAGCATGGA GCAGGCAGCT 
CTGGGAGACA GCGCTGGCTA TATGGAAGTT TCCCTGGATT CCCTGGATCT CCGTGTCAGA 
GGAATTCTGT CTTCCCAGAC AGAAAATGAA GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAATTG 
GGCCGGTGTA CAAACAGCGT GGTCAAGTAT GAGCTGATGC GCCCATCCAA CAAAGCACCG 
CTCTTGGTGT TATGTGAAGA CCATCGGGGT CGCATGGTGA AGCACCAGTG CTGTCCTGGC 
TGCGGCTACT TCTGCACGGC GGGTAACTTC ATGGAATGCC AGCCTGAGAG CAGCATCTCT 
CATCGTTTCC ATAAGGACTG TGCCTCTCGA GTCAACAATG CCAGCTACTG TCCCCATTGT 
GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG ACCATAGCAA AAGCAGACAC AACTTCCACA 
GTGACCCTAG CCCCTGGACA GGAGAAGAGC CTGGCTGCTG AAGGCAGGGC TGACACGACC 
ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT GAAAGATCGC AGAGCACAGC CCCCCAGGCA 
CCAGAGTGCT TCGACCCTGC CGGACCGGCT GGGCTCGTGA GGCCGACATC TGGCCTTTCC 
CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT GCTCTAATCG CTCTAGACTC TGAAAAACCC 
AAGAAACTTC GCTTCCACCC AAAGCAGCTG TACTTCTCTG CCAGGCAGGG TGAGCTGCAG 
AAGGTGCTTC TCATGCTGGT TGATGGAATT GATCCCAACT TCAAAATGGA GCACCAAAGT 
AAGCGTTCCC CATTACATGC TGCTGCGGAG GCTGGCCACG TGGACATCTG CCACATGCTG 
GTTCAGGCGG GTGCCAATAT TGACACTTGC TCAGAGGACC AGCGGACCCC ACTGATGGAG 
GCTGCAGAGA ACAACCACTT GGATGCAGTG AAGTACCTCA TCAAGGCTGG AGCACAGGTG 
GATCCGAAGG ACGCAGAGGG CTCCACATGT TTGCATTTGG CTGCCAAGAA AGGCCACTAT 
GATGTGGTTC AGTATCTGCT TTCAAATGGA CAGATGGATG TCAACTGCCA GGATGACGGT 
GGATGGACAC CTATGATCTG GGCCACTGAG TACAAGCACG TGGAGCTGGT GAAGCTGCTG 
CTGTCTAAGG GCTCTGACAT CAACATCCGG GACAACGAGG AGAACATTTG TCTGCACTGG 
GCAGCATTTT CAGGCTGTGT GGACATAGCT GAAATACTTC TGGCTGCCAA GTGTGACCTG 
CATGCTGTGA ATATCCATGG AGACTCACCC CTGCACATCG CAGCCAGGGA GAATCGCTAC 
GACTGTGTTG TCCTCTTTCT TTCTCGGGAT TCAGATGTTA CTCTGAAAAA CAAGGAAGGA 
GAGACTCCCT TGCAGTGTGC AAGTCTCAGT TCGCAGGTGT GGAGTGCATT GCAGATGAGC 
AAAGCACTTC GGGACTCAGC CCCTGACAAG CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG 
GATATCGCTC GAGGGTATGA GCGCATTCCC ATTCCCTGTG TCAATGCTGT GGACAGTGAG 
CTGTGTCCTA CCAACTATAA GTATGTCTCC CAGAACTGTG TGACATCCCC CATGAACATT 
GACAGGAACA TCACTCATTT GCAGTACTGC GTGTGTGTAG ATGACTGCTC CTCTAGCACC 
TGCATGTGTG GCCAGCTGAG CATGCGCTGC TGGTATGATA AGGATGGCCG ACTTCTGCCT 
GAGTTTAACA TGGCAGAACC ACCCTTGATC TTCGAGTGCA ATCATGCCTG CTCATGCTGG 
AGGAACTGCC GCAATCGTGT GGTGCAAAAT GGTCTCAGGG CAAGGCTGCA GCTTTATCGG 
ACACAGGACA TGGGCTGGGG TGTGCGGTCC CTCCAGGATA TCCCACTGGG CACCTTTGTC 
TGCGAATACG TAGGGGAGCT GATTTCGGAC TCTGAAGCTG ATGTTCGGGA AGAGGACTCT 
TACCTCTTTG ATCTTGACAA TAAGGATGGA GAGGTATACT GCATTGACGC TCGGTTCTAT 
GGGAATGTCA GCCGGTTCAT AAACCACCAC TGCGAACCCA ACCTTGTGCC TGTGCGAGTG 
TTCATGTCAC ACCAGGACCT GCGGTTTCCC AGGATTGCCT TCTTCAGTAC CCGCCTGATT 
CAGGCTGGGG AGCAGCTCGG GTTCGACTAC GGGGAGCGCT TTTGGGACGT CAAGGGCAAG 
CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG TGTCGGCACT CAAGCGCAGC CCTGGCCCAG 
AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG GAGAATGGCC TTCCAGATAC CAGCTCTGCA 
GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_017319336.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174825.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X27, mRNA.

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CloneID	OMu80466
Clone ID Related Accession (Same CDS sequence)	XM_017319336.1
Accession Version	XM_017319336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174825.1
CDS	27..3776
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_017319336.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319336.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108067.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu88044
Clone ID Related Accession (Same CDS sequence)	XM_030252207.1
Accession Version	XM_030252207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA ATTGGGCCGG 
TGTACAAACA GCGTGGTCAA GTATGAGCTG ATGCGCCCAT CCAACAAAGC ACCGCTCTTG 
GTGTTATGTG AAGACCATCG GGGTCGCATG GTGAAGCACC AGTGCTGTCC TGGCTGCGGC 
TACTTCTGCA CGGCGGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108067.1
CDS	18..3932
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252207.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA ATTGGGCCGG 
TGTACAAACA GCGTGGTCAA GTATGAGCTG ATGCGCCCAT CCAACAAAGC ACCGCTCTTG 
GTGTTATGTG AAGACCATCG GGGTCGCATG GTGAAGCACC AGTGCTGTCC TGGCTGCGGC 
TACTTCTGCA CGGCGGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108068.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu88045
Clone ID Related Accession (Same CDS sequence)	XM_030252208.1
Accession Version	XM_030252208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCTCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGCT ATATGGAAGT TTCCCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CAGAAAATGA AGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108068.1
CDS	18..3929
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252208.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCTCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGCT ATATGGAAGT TTCCCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CAGAAAATGA AGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252209.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108069.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X8, mRNA.

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CloneID	OMu88046
Clone ID Related Accession (Same CDS sequence)	XM_030252209.1
Accession Version	XM_030252209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAATTGGG CCGGTGTACA 
AACAGCGTGG TCAAGTATGA GCTGATGCGC CCATCCAACA AAGCACCGCT CTTGGTGTTA 
TGTGAAGACC ATCGGGGTCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG CGGCTACTTC 
TGCACGGCGG GTAACTTCAT GGAATGCCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAAGCT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACAA CTTCCACAGT GACCCTAGCC 
CCTGGACAGG AGAAGAGCCT GGCTGCTGAA GGCAGGGCTG ACACGACCAC GGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAGATCGCAG AGCACAGCCC CCCAGGCACC AGAGTGCTTC 
GACCCTGCCG GACCGGCTGG GCTCGTGAGG CCGACATCTG GCCTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTAATCGCT CTAGACTCTG AAAAACCCAA GAAACTTCGC 
TTCCACCCAA AGCAGCTGTA CTTCTCTGCC AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACATGCTG CTGCGGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG CGGACCCCAC TGATGGAGGC TGCAGAGAAC 
AACCACTTGG ATGCAGTGAA GTACCTCATC AAGGCTGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACATGTTT GCATTTGGCT GCCAAGAAAG GCCACTATGA TGTGGTTCAG 
TATCTGCTTT CAAATGGACA GATGGATGTC AACTGCCAGG ATGACGGTGG ATGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC 
TCTGACATCA ACATCCGGGA CAACGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATACTTCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCATGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT CTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG AGTGCATTGC AGATGAGCAA AGCACTTCGG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGGGA TATCGCTCGA 
GGGTATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ACAGTGAGCT GTGTCCTACC 
AACTATAAGT ATGTCTCCCA GAACTGTGTG ACATCCCCCA TGAACATTGA CAGGAACATC 
ACTCATTTGC AGTACTGCGT GTGTGTAGAT GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGATAAG GATGGCCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CATGCTGGAG GAACTGCCGC 
AATCGTGTGG TGCAAAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT CCAGGATATC CCACTGGGCA CCTTTGTCTG CGAATACGTA 
GGGGAGCTGA TTTCGGACTC TGAAGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTATACTGC ATTGACGCTC GGTTCTATGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAACCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTGC GGTTTCCCAG GATTGCCTTC TTCAGTACCC GCCTGATTCA GGCTGGGGAG 
CAGCTCGGGT TCGACTACGG GGAGCGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTTCCAAGTG TCGGCACTCA AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT 
GCAGCCCAGG AGCCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC CGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108069.1
CDS	18..3926
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252209.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252209.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAATTGGG CCGGTGTACA 
AACAGCGTGG TCAAGTATGA GCTGATGCGC CCATCCAACA AAGCACCGCT CTTGGTGTTA 
TGTGAAGACC ATCGGGGTCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG CGGCTACTTC 
TGCACGGCGG GTAACTTCAT GGAATGCCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAAGCT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACAA CTTCCACAGT GACCCTAGCC 
CCTGGACAGG AGAAGAGCCT GGCTGCTGAA GGCAGGGCTG ACACGACCAC GGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAGATCGCAG AGCACAGCCC CCCAGGCACC AGAGTGCTTC 
GACCCTGCCG GACCGGCTGG GCTCGTGAGG CCGACATCTG GCCTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTAATCGCT CTAGACTCTG AAAAACCCAA GAAACTTCGC 
TTCCACCCAA AGCAGCTGTA CTTCTCTGCC AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACATGCTG CTGCGGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG CGGACCCCAC TGATGGAGGC TGCAGAGAAC 
AACCACTTGG ATGCAGTGAA GTACCTCATC AAGGCTGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACATGTTT GCATTTGGCT GCCAAGAAAG GCCACTATGA TGTGGTTCAG 
TATCTGCTTT CAAATGGACA GATGGATGTC AACTGCCAGG ATGACGGTGG ATGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC 
TCTGACATCA ACATCCGGGA CAACGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATACTTCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCATGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT CTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG AGTGCATTGC AGATGAGCAA AGCACTTCGG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGGGA TATCGCTCGA 
GGGTATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ACAGTGAGCT GTGTCCTACC 
AACTATAAGT ATGTCTCCCA GAACTGTGTG ACATCCCCCA TGAACATTGA CAGGAACATC 
ACTCATTTGC AGTACTGCGT GTGTGTAGAT GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGATAAG GATGGCCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CATGCTGGAG GAACTGCCGC 
AATCGTGTGG TGCAAAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT CCAGGATATC CCACTGGGCA CCTTTGTCTG CGAATACGTA 
GGGGAGCTGA TTTCGGACTC TGAAGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTATACTGC ATTGACGCTC GGTTCTATGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAACCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTGC GGTTTCCCAG GATTGCCTTC TTCAGTACCC GCCTGATTCA GGCTGGGGAG 
CAGCTCGGGT TCGACTACGG GGAGCGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTTCCAAGTG TCGGCACTCA AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT 
GCAGCCCAGG AGCCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC CGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252210.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108070.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu88047
Clone ID Related Accession (Same CDS sequence)	XM_030252210.1
Accession Version	XM_030252210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAATTGGG CCGGTGTACA 
AACAGCGTGG TCAAGTATGA GCTGATGCGC CCATCCAACA AAGCACCGCT CTTGGTGTTA 
TGTGAAGACC ATCGGGGTCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG CGGCTACTTC 
TGCACGGCGG GTAACTTCAT GGAATGCCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAAGCT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACAA CTTCCACAGT GACCCTAGCC 
CCTGGACAGG AGAAGAGCCT GGCTGCTGAA GGCAGGGCTG ACACGACCAC GGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAGATCGCAG AGCACAGCCC CCCAGGCACC AGAGTGCTTC 
GACCCTGCCG GACCGGCTGG GCTCGTGAGG CCGACATCTG GCCTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTAATCGCT CTAGACTCTG AAAAACCCAA GAAACTTCGC 
TTCCACCCAA AGCAGCTGTA CTTCTCTGCC AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACATGCTG CTGCGGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG CGGACCCCAC TGATGGAGGC TGCAGAGAAC 
AACCACTTGG ATGCAGTGAA GTACCTCATC AAGGCTGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACATGTTT GCATTTGGCT GCCAAGAAAG GCCACTATGA TGTGGTTCAG 
TATCTGCTTT CAAATGGACA GATGGATGTC AACTGCCAGG ATGACGGTGG ATGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC 
TCTGACATCA ACATCCGGGA CAACGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATACTTCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCATGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT CTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG AGTGCATTGC AGATGAGCAA AGCACTTCGG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGGGA TATCGCTCGA 
GGGTATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ACAGTGAGCT GTGTCCTACC 
AACTATAAGT ATGTCTCCCA GAACTGTGTG ACATCCCCCA TGAACATTGA CAGGAACATC 
ACTCATTTGC AGTACTGCGT GTGTGTAGAT GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGATAAG GATGGCCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CATGCTGGAG GAACTGCCGC 
AATCGTGTGG TGCAAAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT CCAGGATATC CCACTGGGCA CCTTTGTCTG CGAATACGTA 
GGGGAGCTGA TTTCGGACTC TGAAGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTATACTGC ATTGACGCTC GGTTCTATGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAACCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTGC GGTTTCCCAG GATTGCCTTC TTCAGTACCC GCCTGATTCA GGCTGGGGAG 
CAGCTCGGGT TCGACTACGG GGAGCGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTTCCAAGTG TCGGCACTCA AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT 
GCAGCCCAGG AGCCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC CGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108070.1
CDS	27..3935
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252210.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252210.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAATTGGG CCGGTGTACA 
AACAGCGTGG TCAAGTATGA GCTGATGCGC CCATCCAACA AAGCACCGCT CTTGGTGTTA 
TGTGAAGACC ATCGGGGTCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG CGGCTACTTC 
TGCACGGCGG GTAACTTCAT GGAATGCCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAAGCT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACAA CTTCCACAGT GACCCTAGCC 
CCTGGACAGG AGAAGAGCCT GGCTGCTGAA GGCAGGGCTG ACACGACCAC GGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAGATCGCAG AGCACAGCCC CCCAGGCACC AGAGTGCTTC 
GACCCTGCCG GACCGGCTGG GCTCGTGAGG CCGACATCTG GCCTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTAATCGCT CTAGACTCTG AAAAACCCAA GAAACTTCGC 
TTCCACCCAA AGCAGCTGTA CTTCTCTGCC AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACATGCTG CTGCGGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG CGGACCCCAC TGATGGAGGC TGCAGAGAAC 
AACCACTTGG ATGCAGTGAA GTACCTCATC AAGGCTGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACATGTTT GCATTTGGCT GCCAAGAAAG GCCACTATGA TGTGGTTCAG 
TATCTGCTTT CAAATGGACA GATGGATGTC AACTGCCAGG ATGACGGTGG ATGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC 
TCTGACATCA ACATCCGGGA CAACGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATACTTCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCATGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT CTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG AGTGCATTGC AGATGAGCAA AGCACTTCGG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGGGA TATCGCTCGA 
GGGTATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ACAGTGAGCT GTGTCCTACC 
AACTATAAGT ATGTCTCCCA GAACTGTGTG ACATCCCCCA TGAACATTGA CAGGAACATC 
ACTCATTTGC AGTACTGCGT GTGTGTAGAT GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGATAAG GATGGCCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CATGCTGGAG GAACTGCCGC 
AATCGTGTGG TGCAAAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT CCAGGATATC CCACTGGGCA CCTTTGTCTG CGAATACGTA 
GGGGAGCTGA TTTCGGACTC TGAAGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTATACTGC ATTGACGCTC GGTTCTATGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAACCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTGC GGTTTCCCAG GATTGCCTTC TTCAGTACCC GCCTGATTCA GGCTGGGGAG 
CAGCTCGGGT TCGACTACGG GGAGCGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTTCCAAGTG TCGGCACTCA AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT 
GCAGCCCAGG AGCCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC CGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252211.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108071.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu88048
Clone ID Related Accession (Same CDS sequence)	XM_030252211.1
Accession Version	XM_030252211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTG AGCTGCTAAG GGAAGGTAAA GATACACCTA TGGCTGCTGA TGAAGGTTCC 
ACAGAGAAAC AAGAAGGAGA GACTCCCATG GCTGCAGATG GAGAAACAAA TGGGTCTTGT 
GAAAAGAGTG GGGATCCCAG CCATCTAAAT GCACCCAAAC ACACTCAGGA GAACACAAGA 
GCTAGCCCAC AGGAAGGCAC CAACAGAGTG TCTCGGGTGG CAGAAAATGG GGTTTCAGAA 
AGAGACACAG AAGTGGGGAA GCAAAACCAT GTCACAGCTG ACGACTTCAT GCAGACATCT 
GTCATTGGCA GCAATGGATA TTTCTTAAAT AAACCAGCCC TGCAGGGGCA GCCGTTGAGG 
ACTCCCAACA TTCTAACCTC CTCGCTTCCT GGTCATGCTG CAAAAACTCT TCCTGGAGGA 
GCCAGTAAAT GCAGGACTCT GAGTGCACTT CCTCAGACAC CAACCACAGC ACCCACTGTG 
CCTGGGGAAG GGAGTGCAGA CACAGAGGAC AGAAAGCCTA CAGCCTCGGG CACTGATGTC 
AGGGTTCACA GGGCACGCAA GACCATGCCG AAGTCCATCT TGGGCCTGCA TGCAGCCAGC 
AAAGACCATA GAGAAGTTCA AGACCATAAG GAACCAAAAG AGGATATCAA CAGAAACATT 
TCTGAATGTG GACGACAGCA GCTTTTACCA ACCTTCCCAG CCCTCCACCA GTCGCTACCT 
CAGAATCAGT GCTACATGGC CACCACAAAG TCCCAGACAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGGGC 
GAGAAAGCCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCA 
GAAGATGAAG ACTCCGATGA GCTGGAGGAT GACGAGGACC ATGGAGCTGA GCAAGCGGCT 
GCATTTCCCA CCGAGGATAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGACCGGGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CCGACACAGG GGAGGATGAA 
GATGGTGGAG ACGAGTCTGA CCTGAGTTCT GAATCCAGTA TCAAGAAGAA ATTTCTCAAG 
AGGAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGT 
AGAAAGAAGC CGAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCTCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGC TATATGGAAG TTTCCCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACAGAAAATG AAGGGCTGGC CAGTGGTCCG 
GATGTGCTGG GGACGGATGG CCTCCAGGAA GTGCCTCTCT GCAGCTGCCG AATGGAAACC 
CCCAAGAGCC GCGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCATCC 
AACAAAGCAC CGCTCTTGGT GTTATGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG CCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA AGCTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACAACTTCCA CAGTGACCCT AGCCCCTGGA CAGGAGAAGA GCCTGGCTGC TGAAGGCAGG 
GCTGACACGA CCACGGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAGATC GCAGAGCACA 
GCCCCCCAGG CACCAGAGTG CTTCGACCCT GCCGGACCGG CTGGGCTCGT GAGGCCGACA 
TCTGGCCTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTAAT CGCTCTAGAC 
TCTGAAAAAC CCAAGAAACT TCGCTTCCAC CCAAAGCAGC TGTACTTCTC TGCCAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAT GCTGCTGCGG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGCGGACC 
CCACTGATGG AGGCTGCAGA GAACAACCAC TTGGATGCAG TGAAGTACCT CATCAAGGCT 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACAT GTTTGCATTT GGCTGCCAAG 
AAAGGCCACT ATGATGTGGT TCAGTATCTG CTTTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGACG GTGGATGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGAGCTG 
GTGAAGCTGC TGCTGTCTAA GGGCTCTGAC ATCAACATCC GGGACAACGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATACT TCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAT GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTCTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGTCTCA GTTCGCAGGT GTGGAGTGCA 
TTGCAGATGA GCAAAGCACT TCGGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GGGATATCGC TCGAGGGTAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGACAGTG AGCTGTGTCC TACCAACTAT AAGTATGTCT CCCAGAACTG TGTGACATCC 
CCCATGAACA TTGACAGGAA CATCACTCAT TTGCAGTACT GCGTGTGTGT AGATGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA TAAGGATGGC 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCATGCT GGAGGAACTG CCGCAATCGT GTGGTGCAAA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTCCAGGA TATCCCACTG 
GGCACCTTTG TCTGCGAATA CGTAGGGGAG CTGATTTCGG ACTCTGAAGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTATA CTGCATTGAC 
GCTCGGTTCT ATGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAACC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTGCGGTTTC CCAGGATTGC CTTCTTCAGT 
ACCCGCCTGA TTCAGGCTGG GGAGCAGCTC GGGTTCGACT ACGGGGAGCG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTTCCA AGTGTCGGCA CTCAAGCGCA 
GCCCTGGCCC AGAGGCAAGC CAGTGCAGCC CAGGAGCCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCCGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108071.1
CDS	376..4188
Translation	MKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252211.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252211.1

ORF Insert Sequence:

ATGAAAACTG AGCTGCTAAG GGAAGGTAAA GATACACCTA TGGCTGCTGA TGAAGGTTCC 
ACAGAGAAAC AAGAAGGAGA GACTCCCATG GCTGCAGATG GAGAAACAAA TGGGTCTTGT 
GAAAAGAGTG GGGATCCCAG CCATCTAAAT GCACCCAAAC ACACTCAGGA GAACACAAGA 
GCTAGCCCAC AGGAAGGCAC CAACAGAGTG TCTCGGGTGG CAGAAAATGG GGTTTCAGAA 
AGAGACACAG AAGTGGGGAA GCAAAACCAT GTCACAGCTG ACGACTTCAT GCAGACATCT 
GTCATTGGCA GCAATGGATA TTTCTTAAAT AAACCAGCCC TGCAGGGGCA GCCGTTGAGG 
ACTCCCAACA TTCTAACCTC CTCGCTTCCT GGTCATGCTG CAAAAACTCT TCCTGGAGGA 
GCCAGTAAAT GCAGGACTCT GAGTGCACTT CCTCAGACAC CAACCACAGC ACCCACTGTG 
CCTGGGGAAG GGAGTGCAGA CACAGAGGAC AGAAAGCCTA CAGCCTCGGG CACTGATGTC 
AGGGTTCACA GGGCACGCAA GACCATGCCG AAGTCCATCT TGGGCCTGCA TGCAGCCAGC 
AAAGACCATA GAGAAGTTCA AGACCATAAG GAACCAAAAG AGGATATCAA CAGAAACATT 
TCTGAATGTG GACGACAGCA GCTTTTACCA ACCTTCCCAG CCCTCCACCA GTCGCTACCT 
CAGAATCAGT GCTACATGGC CACCACAAAG TCCCAGACAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGGGC 
GAGAAAGCCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCA 
GAAGATGAAG ACTCCGATGA GCTGGAGGAT GACGAGGACC ATGGAGCTGA GCAAGCGGCT 
GCATTTCCCA CCGAGGATAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGACCGGGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CCGACACAGG GGAGGATGAA 
GATGGTGGAG ACGAGTCTGA CCTGAGTTCT GAATCCAGTA TCAAGAAGAA ATTTCTCAAG 
AGGAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGT 
AGAAAGAAGC CGAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCTCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGC TATATGGAAG TTTCCCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACAGAAAATG AAGGGCTGGC CAGTGGTCCG 
GATGTGCTGG GGACGGATGG CCTCCAGGAA GTGCCTCTCT GCAGCTGCCG AATGGAAACC 
CCCAAGAGCC GCGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCATCC 
AACAAAGCAC CGCTCTTGGT GTTATGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG CCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA AGCTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACAACTTCCA CAGTGACCCT AGCCCCTGGA CAGGAGAAGA GCCTGGCTGC TGAAGGCAGG 
GCTGACACGA CCACGGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAGATC GCAGAGCACA 
GCCCCCCAGG CACCAGAGTG CTTCGACCCT GCCGGACCGG CTGGGCTCGT GAGGCCGACA 
TCTGGCCTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTAAT CGCTCTAGAC 
TCTGAAAAAC CCAAGAAACT TCGCTTCCAC CCAAAGCAGC TGTACTTCTC TGCCAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAT GCTGCTGCGG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGCGGACC 
CCACTGATGG AGGCTGCAGA GAACAACCAC TTGGATGCAG TGAAGTACCT CATCAAGGCT 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACAT GTTTGCATTT GGCTGCCAAG 
AAAGGCCACT ATGATGTGGT TCAGTATCTG CTTTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGACG GTGGATGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGAGCTG 
GTGAAGCTGC TGCTGTCTAA GGGCTCTGAC ATCAACATCC GGGACAACGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATACT TCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAT GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTCTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGTCTCA GTTCGCAGGT GTGGAGTGCA 
TTGCAGATGA GCAAAGCACT TCGGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GGGATATCGC TCGAGGGTAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGACAGTG AGCTGTGTCC TACCAACTAT AAGTATGTCT CCCAGAACTG TGTGACATCC 
CCCATGAACA TTGACAGGAA CATCACTCAT TTGCAGTACT GCGTGTGTGT AGATGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA TAAGGATGGC 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCATGCT GGAGGAACTG CCGCAATCGT GTGGTGCAAA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTCCAGGA TATCCCACTG 
GGCACCTTTG TCTGCGAATA CGTAGGGGAG CTGATTTCGG ACTCTGAAGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTATA CTGCATTGAC 
GCTCGGTTCT ATGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAACC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTGCGGTTTC CCAGGATTGC CTTCTTCAGT 
ACCCGCCTGA TTCAGGCTGG GGAGCAGCTC GGGTTCGACT ACGGGGAGCG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTTCCA AGTGTCGGCA CTCAAGCGCA 
GCCCTGGCCC AGAGGCAAGC CAGTGCAGCC CAGGAGCCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCCGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252212.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108072.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X12, mRNA.

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CloneID	OMu88049
Clone ID Related Accession (Same CDS sequence)	XM_030252212.1 , XM_030252213.1
Accession Version	XM_030252212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GGGCGAGAAA 
GCCTTAGATG ATAGTGCCCT GCATGTAAAT GGCGAGAGCT TGGAGATGGA CTCAGAAGAT 
GAAGACTCCG ATGAGCTGGA GGATGACGAG GACCATGGAG CTGAGCAAGC GGCTGCATTT 
CCCACCGAGG ATAGTAGGAC TTCTAAAGAG AGCATGTCTG AGACTGACCG GGCTGCAAAG 
ATGGATGGAG ATTCAGAGGA GGAACAGGAG TCTCCCGACA CAGGGGAGGA TGAAGATGGT 
GGAGACGAGT CTGACCTGAG TTCTGAATCC AGTATCAAGA AGAAATTTCT CAAGAGGAGA 
GGGAAGACTG ACAGCCCCTG GATCAAACCT GCTCGGAAAA GGAGGCGGAG AAGTAGAAAG 
AAGCCGAGCA GCATGCTTGG TTCCGAGGCT TGTAAGTCAT CTCCAGGAAG CATGGAGCAG 
GCAGCTCTGG GAGACAGCGC TGGCTATATG GAAGTTTCCC TGGATTCCCT GGATCTCCGT 
GTCAGAGGAA TTCTGTCTTC CCAGACAGAA AATGAAGGGC TGGCCAGTGG TCCGGATGTG 
CTGGGGACGG ATGGCCTCCA GGAAGTGCCT CTCTGCAGCT GCCGAATGGA AACCCCCAAG 
AGCCGCGAGA TCAGCACCCT GGCCAACAAC CAGTGCATGG CCACTGAGAG CGTGGATCAC 
GAATTGGGCC GGTGTACAAA CAGCGTGGTC AAGTATGAGC TGATGCGCCC ATCCAACAAA 
GCACCGCTCT TGGTGTTATG TGAAGACCAT CGGGGTCGCA TGGTGAAGCA CCAGTGCTGT 
CCTGGCTGCG GCTACTTCTG CACGGCGGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108072.1
CDS	379..4185
Translation	MKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252212.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252212.1

ORF Insert Sequence:

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GGGCGAGAAA 
GCCTTAGATG ATAGTGCCCT GCATGTAAAT GGCGAGAGCT TGGAGATGGA CTCAGAAGAT 
GAAGACTCCG ATGAGCTGGA GGATGACGAG GACCATGGAG CTGAGCAAGC GGCTGCATTT 
CCCACCGAGG ATAGTAGGAC TTCTAAAGAG AGCATGTCTG AGACTGACCG GGCTGCAAAG 
ATGGATGGAG ATTCAGAGGA GGAACAGGAG TCTCCCGACA CAGGGGAGGA TGAAGATGGT 
GGAGACGAGT CTGACCTGAG TTCTGAATCC AGTATCAAGA AGAAATTTCT CAAGAGGAGA 
GGGAAGACTG ACAGCCCCTG GATCAAACCT GCTCGGAAAA GGAGGCGGAG AAGTAGAAAG 
AAGCCGAGCA GCATGCTTGG TTCCGAGGCT TGTAAGTCAT CTCCAGGAAG CATGGAGCAG 
GCAGCTCTGG GAGACAGCGC TGGCTATATG GAAGTTTCCC TGGATTCCCT GGATCTCCGT 
GTCAGAGGAA TTCTGTCTTC CCAGACAGAA AATGAAGGGC TGGCCAGTGG TCCGGATGTG 
CTGGGGACGG ATGGCCTCCA GGAAGTGCCT CTCTGCAGCT GCCGAATGGA AACCCCCAAG 
AGCCGCGAGA TCAGCACCCT GGCCAACAAC CAGTGCATGG CCACTGAGAG CGTGGATCAC 
GAATTGGGCC GGTGTACAAA CAGCGTGGTC AAGTATGAGC TGATGCGCCC ATCCAACAAA 
GCACCGCTCT TGGTGTTATG TGAAGACCAT CGGGGTCGCA TGGTGAAGCA CCAGTGCTGT 
CCTGGCTGCG GCTACTTCTG CACGGCGGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252213.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108073.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu88049
Clone ID Related Accession (Same CDS sequence)	XM_030252212.1 , XM_030252213.1
Accession Version	XM_030252213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GGGCGAGAAA 
GCCTTAGATG ATAGTGCCCT GCATGTAAAT GGCGAGAGCT TGGAGATGGA CTCAGAAGAT 
GAAGACTCCG ATGAGCTGGA GGATGACGAG GACCATGGAG CTGAGCAAGC GGCTGCATTT 
CCCACCGAGG ATAGTAGGAC TTCTAAAGAG AGCATGTCTG AGACTGACCG GGCTGCAAAG 
ATGGATGGAG ATTCAGAGGA GGAACAGGAG TCTCCCGACA CAGGGGAGGA TGAAGATGGT 
GGAGACGAGT CTGACCTGAG TTCTGAATCC AGTATCAAGA AGAAATTTCT CAAGAGGAGA 
GGGAAGACTG ACAGCCCCTG GATCAAACCT GCTCGGAAAA GGAGGCGGAG AAGTAGAAAG 
AAGCCGAGCA GCATGCTTGG TTCCGAGGCT TGTAAGTCAT CTCCAGGAAG CATGGAGCAG 
GCAGCTCTGG GAGACAGCGC TGGCTATATG GAAGTTTCCC TGGATTCCCT GGATCTCCGT 
GTCAGAGGAA TTCTGTCTTC CCAGACAGAA AATGAAGGGC TGGCCAGTGG TCCGGATGTG 
CTGGGGACGG ATGGCCTCCA GGAAGTGCCT CTCTGCAGCT GCCGAATGGA AACCCCCAAG 
AGCCGCGAGA TCAGCACCCT GGCCAACAAC CAGTGCATGG CCACTGAGAG CGTGGATCAC 
GAATTGGGCC GGTGTACAAA CAGCGTGGTC AAGTATGAGC TGATGCGCCC ATCCAACAAA 
GCACCGCTCT TGGTGTTATG TGAAGACCAT CGGGGTCGCA TGGTGAAGCA CCAGTGCTGT 
CCTGGCTGCG GCTACTTCTG CACGGCGGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108073.1
CDS	376..4182
Translation	MKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252213.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252213.1

ORF Insert Sequence:

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GGGCGAGAAA 
GCCTTAGATG ATAGTGCCCT GCATGTAAAT GGCGAGAGCT TGGAGATGGA CTCAGAAGAT 
GAAGACTCCG ATGAGCTGGA GGATGACGAG GACCATGGAG CTGAGCAAGC GGCTGCATTT 
CCCACCGAGG ATAGTAGGAC TTCTAAAGAG AGCATGTCTG AGACTGACCG GGCTGCAAAG 
ATGGATGGAG ATTCAGAGGA GGAACAGGAG TCTCCCGACA CAGGGGAGGA TGAAGATGGT 
GGAGACGAGT CTGACCTGAG TTCTGAATCC AGTATCAAGA AGAAATTTCT CAAGAGGAGA 
GGGAAGACTG ACAGCCCCTG GATCAAACCT GCTCGGAAAA GGAGGCGGAG AAGTAGAAAG 
AAGCCGAGCA GCATGCTTGG TTCCGAGGCT TGTAAGTCAT CTCCAGGAAG CATGGAGCAG 
GCAGCTCTGG GAGACAGCGC TGGCTATATG GAAGTTTCCC TGGATTCCCT GGATCTCCGT 
GTCAGAGGAA TTCTGTCTTC CCAGACAGAA AATGAAGGGC TGGCCAGTGG TCCGGATGTG 
CTGGGGACGG ATGGCCTCCA GGAAGTGCCT CTCTGCAGCT GCCGAATGGA AACCCCCAAG 
AGCCGCGAGA TCAGCACCCT GGCCAACAAC CAGTGCATGG CCACTGAGAG CGTGGATCAC 
GAATTGGGCC GGTGTACAAA CAGCGTGGTC AAGTATGAGC TGATGCGCCC ATCCAACAAA 
GCACCGCTCT TGGTGTTATG TGAAGACCAT CGGGGTCGCA TGGTGAAGCA CCAGTGCTGT 
CCTGGCTGCG GCTACTTCTG CACGGCGGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108074.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu88050
Clone ID Related Accession (Same CDS sequence)	XM_030252214.1
Accession Version	XM_030252214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108074.1
CDS	18..3809
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252214.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252215.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108075.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X17, mRNA.

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CloneID	OMu88051
Clone ID Related Accession (Same CDS sequence)	XM_030252215.1
Accession Version	XM_030252215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAAGGTA ACTTCATGGA ATGCCAGCCT GAGAGCAGCA TCTCTCATCG TTTCCATAAG 
GACTGTGCCT CTCGAGTCAA CAATGCCAGC TACTGTCCCC ATTGTGGGGA AGAAGCTTCC 
AAGGCCAAAG AGGTGACCAT AGCAAAAGCA GACACAACTT CCACAGTGAC CCTAGCCCCT 
GGACAGGAGA AGAGCCTGGC TGCTGAAGGC AGGGCTGACA CGACCACGGG CAGCATTGCT 
GGAGCCCCAG AGGATGAAAG ATCGCAGAGC ACAGCCCCCC AGGCACCAGA GTGCTTCGAC 
CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG ACATCTGGCC TTTCCCAGGG CCCAGGAAAG 
GAAACCTTGG AAAGTGCTCT AATCGCTCTA GACTCTGAAA AACCCAAGAA ACTTCGCTTC 
CACCCAAAGC AGCTGTACTT CTCTGCCAGG CAGGGTGAGC TGCAGAAGGT GCTTCTCATG 
CTGGTTGATG GAATTGATCC CAACTTCAAA ATGGAGCACC AAAGTAAGCG TTCCCCATTA 
CATGCTGCTG CGGAGGCTGG CCACGTGGAC ATCTGCCACA TGCTGGTTCA GGCGGGTGCC 
AATATTGACA CTTGCTCAGA GGACCAGCGG ACCCCACTGA TGGAGGCTGC AGAGAACAAC 
CACTTGGATG CAGTGAAGTA CCTCATCAAG GCTGGAGCAC AGGTGGATCC GAAGGACGCA 
GAGGGCTCCA CATGTTTGCA TTTGGCTGCC AAGAAAGGCC ACTATGATGT GGTTCAGTAT 
CTGCTTTCAA ATGGACAGAT GGATGTCAAC TGCCAGGATG ACGGTGGATG GACACCTATG 
ATCTGGGCCA CTGAGTACAA GCACGTGGAG CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT 
GACATCAACA TCCGGGACAA CGAGGAGAAC ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC 
TGTGTGGACA TAGCTGAAAT ACTTCTGGCT GCCAAGTGTG ACCTGCATGC TGTGAATATC 
CATGGAGACT CACCCCTGCA CATCGCAGCC AGGGAGAATC GCTACGACTG TGTTGTCCTC 
TTTCTTTCTC GGGATTCAGA TGTTACTCTG AAAAACAAGG AAGGAGAGAC TCCCTTGCAG 
TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT GCATTGCAGA TGAGCAAAGC ACTTCGGGAC 
TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG AAGACGGTGA GCAGGGATAT CGCTCGAGGG 
TATGAGCGCA TTCCCATTCC CTGTGTCAAT GCTGTGGACA GTGAGCTGTG TCCTACCAAC 
TATAAGTATG TCTCCCAGAA CTGTGTGACA TCCCCCATGA ACATTGACAG GAACATCACT 
CATTTGCAGT ACTGCGTGTG TGTAGATGAC TGCTCCTCTA GCACCTGCAT GTGTGGCCAG 
CTGAGCATGC GCTGCTGGTA TGATAAGGAT GGCCGACTTC TGCCTGAGTT TAACATGGCA 
GAACCACCCT TGATCTTCGA GTGCAATCAT GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT 
CGTGTGGTGC AAAATGGTCT CAGGGCAAGG CTGCAGCTTT ATCGGACACA GGACATGGGC 
TGGGGTGTGC GGTCCCTCCA GGATATCCCA CTGGGCACCT TTGTCTGCGA ATACGTAGGG 
GAGCTGATTT CGGACTCTGA AGCTGATGTT CGGGAAGAGG ACTCTTACCT CTTTGATCTT 
GACAATAAGG ATGGAGAGGT ATACTGCATT GACGCTCGGT TCTATGGGAA TGTCAGCCGG 
TTCATAAACC ACCACTGCGA ACCCAACCTT GTGCCTGTGC GAGTGTTCAT GTCACACCAG 
GACCTGCGGT TTCCCAGGAT TGCCTTCTTC AGTACCCGCC TGATTCAGGC TGGGGAGCAG 
CTCGGGTTCG ACTACGGGGA GCGCTTTTGG GACGTCAAGG GCAAGCTCTT CAGTTGCCGG 
TGTGGGTCTT CCAAGTGTCG GCACTCAAGC GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA 
GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA GATACCAGCT CTGCAGCCGC TGCTGACCCC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108075.1
CDS	18..3803
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252215.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252215.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAAGGTA ACTTCATGGA ATGCCAGCCT GAGAGCAGCA TCTCTCATCG TTTCCATAAG 
GACTGTGCCT CTCGAGTCAA CAATGCCAGC TACTGTCCCC ATTGTGGGGA AGAAGCTTCC 
AAGGCCAAAG AGGTGACCAT AGCAAAAGCA GACACAACTT CCACAGTGAC CCTAGCCCCT 
GGACAGGAGA AGAGCCTGGC TGCTGAAGGC AGGGCTGACA CGACCACGGG CAGCATTGCT 
GGAGCCCCAG AGGATGAAAG ATCGCAGAGC ACAGCCCCCC AGGCACCAGA GTGCTTCGAC 
CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG ACATCTGGCC TTTCCCAGGG CCCAGGAAAG 
GAAACCTTGG AAAGTGCTCT AATCGCTCTA GACTCTGAAA AACCCAAGAA ACTTCGCTTC 
CACCCAAAGC AGCTGTACTT CTCTGCCAGG CAGGGTGAGC TGCAGAAGGT GCTTCTCATG 
CTGGTTGATG GAATTGATCC CAACTTCAAA ATGGAGCACC AAAGTAAGCG TTCCCCATTA 
CATGCTGCTG CGGAGGCTGG CCACGTGGAC ATCTGCCACA TGCTGGTTCA GGCGGGTGCC 
AATATTGACA CTTGCTCAGA GGACCAGCGG ACCCCACTGA TGGAGGCTGC AGAGAACAAC 
CACTTGGATG CAGTGAAGTA CCTCATCAAG GCTGGAGCAC AGGTGGATCC GAAGGACGCA 
GAGGGCTCCA CATGTTTGCA TTTGGCTGCC AAGAAAGGCC ACTATGATGT GGTTCAGTAT 
CTGCTTTCAA ATGGACAGAT GGATGTCAAC TGCCAGGATG ACGGTGGATG GACACCTATG 
ATCTGGGCCA CTGAGTACAA GCACGTGGAG CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT 
GACATCAACA TCCGGGACAA CGAGGAGAAC ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC 
TGTGTGGACA TAGCTGAAAT ACTTCTGGCT GCCAAGTGTG ACCTGCATGC TGTGAATATC 
CATGGAGACT CACCCCTGCA CATCGCAGCC AGGGAGAATC GCTACGACTG TGTTGTCCTC 
TTTCTTTCTC GGGATTCAGA TGTTACTCTG AAAAACAAGG AAGGAGAGAC TCCCTTGCAG 
TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT GCATTGCAGA TGAGCAAAGC ACTTCGGGAC 
TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG AAGACGGTGA GCAGGGATAT CGCTCGAGGG 
TATGAGCGCA TTCCCATTCC CTGTGTCAAT GCTGTGGACA GTGAGCTGTG TCCTACCAAC 
TATAAGTATG TCTCCCAGAA CTGTGTGACA TCCCCCATGA ACATTGACAG GAACATCACT 
CATTTGCAGT ACTGCGTGTG TGTAGATGAC TGCTCCTCTA GCACCTGCAT GTGTGGCCAG 
CTGAGCATGC GCTGCTGGTA TGATAAGGAT GGCCGACTTC TGCCTGAGTT TAACATGGCA 
GAACCACCCT TGATCTTCGA GTGCAATCAT GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT 
CGTGTGGTGC AAAATGGTCT CAGGGCAAGG CTGCAGCTTT ATCGGACACA GGACATGGGC 
TGGGGTGTGC GGTCCCTCCA GGATATCCCA CTGGGCACCT TTGTCTGCGA ATACGTAGGG 
GAGCTGATTT CGGACTCTGA AGCTGATGTT CGGGAAGAGG ACTCTTACCT CTTTGATCTT 
GACAATAAGG ATGGAGAGGT ATACTGCATT GACGCTCGGT TCTATGGGAA TGTCAGCCGG 
TTCATAAACC ACCACTGCGA ACCCAACCTT GTGCCTGTGC GAGTGTTCAT GTCACACCAG 
GACCTGCGGT TTCCCAGGAT TGCCTTCTTC AGTACCCGCC TGATTCAGGC TGGGGAGCAG 
CTCGGGTTCG ACTACGGGGA GCGCTTTTGG GACGTCAAGG GCAAGCTCTT CAGTTGCCGG 
TGTGGGTCTT CCAAGTGTCG GCACTCAAGC GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA 
GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA GATACCAGCT CTGCAGCCGC TGCTGACCCC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252216.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108076.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X19, mRNA.

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CloneID	OMu88052
Clone ID Related Accession (Same CDS sequence)	XM_030252216.1
Accession Version	XM_030252216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGGCTGG CCAGTGGTCC GGATGTGCTG GGGACGGATG GCCTCCAGGA AGTGCCTCTC 
TGCAGCTGCC GAATGGAAAC CCCCAAGAGC CGCGAGATCA GCACCCTGGC CAACAACCAG 
TGCATGGCCA CTGAGAGCGT GGATCACGAA TTGGGCCGGT GTACAAACAG CGTGGTCAAG 
TATGAGCTGA TGCGCCCATC CAACAAAGCA CCGCTCTTGG TGTTATGTGA AGACCATCGG 
GGTCGCATGG TGAAGCACCA GTGCTGTCCT GGCTGCGGCT ACTTCTGCAC GGCGGGTAAC 
TTCATGGAAT GCCAGCCTGA GAGCAGCATC TCTCATCGTT TCCATAAGGA CTGTGCCTCT 
CGAGTCAACA ATGCCAGCTA CTGTCCCCAT TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG 
GTGACCATAG CAAAAGCAGA CACAACTTCC ACAGTGACCC TAGCCCCTGG ACAGGAGAAG 
AGCCTGGCTG CTGAAGGCAG GGCTGACACG ACCACGGGCA GCATTGCTGG AGCCCCAGAG 
GATGAAAGAT CGCAGAGCAC AGCCCCCCAG GCACCAGAGT GCTTCGACCC TGCCGGACCG 
GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT TCCCAGGGCC CAGGAAAGGA AACCTTGGAA 
AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG 
CTGTACTTCT CTGCCAGGCA GGGTGAGCTG CAGAAGGTGC TTCTCATGCT GGTTGATGGA 
ATTGATCCCA ACTTCAAAAT GGAGCACCAA AGTAAGCGTT CCCCATTACA TGCTGCTGCG 
GAGGCTGGCC ACGTGGACAT CTGCCACATG CTGGTTCAGG CGGGTGCCAA TATTGACACT 
TGCTCAGAGG ACCAGCGGAC CCCACTGATG GAGGCTGCAG AGAACAACCA CTTGGATGCA 
GTGAAGTACC TCATCAAGGC TGGAGCACAG GTGGATCCGA AGGACGCAGA GGGCTCCACA 
TGTTTGCATT TGGCTGCCAA GAAAGGCCAC TATGATGTGG TTCAGTATCT GCTTTCAAAT 
GGACAGATGG ATGTCAACTG CCAGGATGAC GGTGGATGGA CACCTATGAT CTGGGCCACT 
GAGTACAAGC ACGTGGAGCT GGTGAAGCTG CTGCTGTCTA AGGGCTCTGA CATCAACATC 
CGGGACAACG AGGAGAACAT TTGTCTGCAC TGGGCAGCAT TTTCAGGCTG TGTGGACATA 
GCTGAAATAC TTCTGGCTGC CAAGTGTGAC CTGCATGCTG TGAATATCCA TGGAGACTCA 
CCCCTGCACA TCGCAGCCAG GGAGAATCGC TACGACTGTG TTGTCCTCTT TCTTTCTCGG 
GATTCAGATG TTACTCTGAA AAACAAGGAA GGAGAGACTC CCTTGCAGTG TGCAAGTCTC 
AGTTCGCAGG TGTGGAGTGC ATTGCAGATG AGCAAAGCAC TTCGGGACTC AGCCCCTGAC 
AAGCCCGTTG CTGTTGAGAA GACGGTGAGC AGGGATATCG CTCGAGGGTA TGAGCGCATT 
CCCATTCCCT GTGTCAATGC TGTGGACAGT GAGCTGTGTC CTACCAACTA TAAGTATGTC 
TCCCAGAACT GTGTGACATC CCCCATGAAC ATTGACAGGA ACATCACTCA TTTGCAGTAC 
TGCGTGTGTG TAGATGACTG CTCCTCTAGC ACCTGCATGT GTGGCCAGCT GAGCATGCGC 
TGCTGGTATG ATAAGGATGG CCGACTTCTG CCTGAGTTTA ACATGGCAGA ACCACCCTTG 
ATCTTCGAGT GCAATCATGC CTGCTCATGC TGGAGGAACT GCCGCAATCG TGTGGTGCAA 
AATGGTCTCA GGGCAAGGCT GCAGCTTTAT CGGACACAGG ACATGGGCTG GGGTGTGCGG 
TCCCTCCAGG ATATCCCACT GGGCACCTTT GTCTGCGAAT ACGTAGGGGA GCTGATTTCG 
GACTCTGAAG CTGATGTTCG GGAAGAGGAC TCTTACCTCT TTGATCTTGA CAATAAGGAT 
GGAGAGGTAT ACTGCATTGA CGCTCGGTTC TATGGGAATG TCAGCCGGTT CATAAACCAC 
CACTGCGAAC CCAACCTTGT GCCTGTGCGA GTGTTCATGT CACACCAGGA CCTGCGGTTT 
CCCAGGATTG CCTTCTTCAG TACCCGCCTG ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC 
TACGGGGAGC GCTTTTGGGA CGTCAAGGGC AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC 
AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT 
CAGGAGAATG GCCTTCCAGA TACCAGCTCT GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108076.1
CDS	18..3791
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252216.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252216.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGGCTGG CCAGTGGTCC GGATGTGCTG GGGACGGATG GCCTCCAGGA AGTGCCTCTC 
TGCAGCTGCC GAATGGAAAC CCCCAAGAGC CGCGAGATCA GCACCCTGGC CAACAACCAG 
TGCATGGCCA CTGAGAGCGT GGATCACGAA TTGGGCCGGT GTACAAACAG CGTGGTCAAG 
TATGAGCTGA TGCGCCCATC CAACAAAGCA CCGCTCTTGG TGTTATGTGA AGACCATCGG 
GGTCGCATGG TGAAGCACCA GTGCTGTCCT GGCTGCGGCT ACTTCTGCAC GGCGGGTAAC 
TTCATGGAAT GCCAGCCTGA GAGCAGCATC TCTCATCGTT TCCATAAGGA CTGTGCCTCT 
CGAGTCAACA ATGCCAGCTA CTGTCCCCAT TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG 
GTGACCATAG CAAAAGCAGA CACAACTTCC ACAGTGACCC TAGCCCCTGG ACAGGAGAAG 
AGCCTGGCTG CTGAAGGCAG GGCTGACACG ACCACGGGCA GCATTGCTGG AGCCCCAGAG 
GATGAAAGAT CGCAGAGCAC AGCCCCCCAG GCACCAGAGT GCTTCGACCC TGCCGGACCG 
GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT TCCCAGGGCC CAGGAAAGGA AACCTTGGAA 
AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG 
CTGTACTTCT CTGCCAGGCA GGGTGAGCTG CAGAAGGTGC TTCTCATGCT GGTTGATGGA 
ATTGATCCCA ACTTCAAAAT GGAGCACCAA AGTAAGCGTT CCCCATTACA TGCTGCTGCG 
GAGGCTGGCC ACGTGGACAT CTGCCACATG CTGGTTCAGG CGGGTGCCAA TATTGACACT 
TGCTCAGAGG ACCAGCGGAC CCCACTGATG GAGGCTGCAG AGAACAACCA CTTGGATGCA 
GTGAAGTACC TCATCAAGGC TGGAGCACAG GTGGATCCGA AGGACGCAGA GGGCTCCACA 
TGTTTGCATT TGGCTGCCAA GAAAGGCCAC TATGATGTGG TTCAGTATCT GCTTTCAAAT 
GGACAGATGG ATGTCAACTG CCAGGATGAC GGTGGATGGA CACCTATGAT CTGGGCCACT 
GAGTACAAGC ACGTGGAGCT GGTGAAGCTG CTGCTGTCTA AGGGCTCTGA CATCAACATC 
CGGGACAACG AGGAGAACAT TTGTCTGCAC TGGGCAGCAT TTTCAGGCTG TGTGGACATA 
GCTGAAATAC TTCTGGCTGC CAAGTGTGAC CTGCATGCTG TGAATATCCA TGGAGACTCA 
CCCCTGCACA TCGCAGCCAG GGAGAATCGC TACGACTGTG TTGTCCTCTT TCTTTCTCGG 
GATTCAGATG TTACTCTGAA AAACAAGGAA GGAGAGACTC CCTTGCAGTG TGCAAGTCTC 
AGTTCGCAGG TGTGGAGTGC ATTGCAGATG AGCAAAGCAC TTCGGGACTC AGCCCCTGAC 
AAGCCCGTTG CTGTTGAGAA GACGGTGAGC AGGGATATCG CTCGAGGGTA TGAGCGCATT 
CCCATTCCCT GTGTCAATGC TGTGGACAGT GAGCTGTGTC CTACCAACTA TAAGTATGTC 
TCCCAGAACT GTGTGACATC CCCCATGAAC ATTGACAGGA ACATCACTCA TTTGCAGTAC 
TGCGTGTGTG TAGATGACTG CTCCTCTAGC ACCTGCATGT GTGGCCAGCT GAGCATGCGC 
TGCTGGTATG ATAAGGATGG CCGACTTCTG CCTGAGTTTA ACATGGCAGA ACCACCCTTG 
ATCTTCGAGT GCAATCATGC CTGCTCATGC TGGAGGAACT GCCGCAATCG TGTGGTGCAA 
AATGGTCTCA GGGCAAGGCT GCAGCTTTAT CGGACACAGG ACATGGGCTG GGGTGTGCGG 
TCCCTCCAGG ATATCCCACT GGGCACCTTT GTCTGCGAAT ACGTAGGGGA GCTGATTTCG 
GACTCTGAAG CTGATGTTCG GGAAGAGGAC TCTTACCTCT TTGATCTTGA CAATAAGGAT 
GGAGAGGTAT ACTGCATTGA CGCTCGGTTC TATGGGAATG TCAGCCGGTT CATAAACCAC 
CACTGCGAAC CCAACCTTGT GCCTGTGCGA GTGTTCATGT CACACCAGGA CCTGCGGTTT 
CCCAGGATTG CCTTCTTCAG TACCCGCCTG ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC 
TACGGGGAGC GCTTTTGGGA CGTCAAGGGC AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC 
AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT 
CAGGAGAATG GCCTTCCAGA TACCAGCTCT GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252217.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108077.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu88053
Clone ID Related Accession (Same CDS sequence)	XM_030252217.1
Accession Version	XM_030252217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAAGGTAAC 
TTCATGGAAT GCCAGCCTGA GAGCAGCATC TCTCATCGTT TCCATAAGGA CTGTGCCTCT 
CGAGTCAACA ATGCCAGCTA CTGTCCCCAT TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG 
GTGACCATAG CAAAAGCAGA CACAACTTCC ACAGTGACCC TAGCCCCTGG ACAGGAGAAG 
AGCCTGGCTG CTGAAGGCAG GGCTGACACG ACCACGGGCA GCATTGCTGG AGCCCCAGAG 
GATGAAAGAT CGCAGAGCAC AGCCCCCCAG GCACCAGAGT GCTTCGACCC TGCCGGACCG 
GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT TCCCAGGGCC CAGGAAAGGA AACCTTGGAA 
AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG 
CTGTACTTCT CTGCCAGGCA GGGTGAGCTG CAGAAGGTGC TTCTCATGCT GGTTGATGGA 
ATTGATCCCA ACTTCAAAAT GGAGCACCAA AGTAAGCGTT CCCCATTACA TGCTGCTGCG 
GAGGCTGGCC ACGTGGACAT CTGCCACATG CTGGTTCAGG CGGGTGCCAA TATTGACACT 
TGCTCAGAGG ACCAGCGGAC CCCACTGATG GAGGCTGCAG AGAACAACCA CTTGGATGCA 
GTGAAGTACC TCATCAAGGC TGGAGCACAG GTGGATCCGA AGGACGCAGA GGGCTCCACA 
TGTTTGCATT TGGCTGCCAA GAAAGGCCAC TATGATGTGG TTCAGTATCT GCTTTCAAAT 
GGACAGATGG ATGTCAACTG CCAGGATGAC GGTGGATGGA CACCTATGAT CTGGGCCACT 
GAGTACAAGC ACGTGGAGCT GGTGAAGCTG CTGCTGTCTA AGGGCTCTGA CATCAACATC 
CGGGACAACG AGGAGAACAT TTGTCTGCAC TGGGCAGCAT TTTCAGGCTG TGTGGACATA 
GCTGAAATAC TTCTGGCTGC CAAGTGTGAC CTGCATGCTG TGAATATCCA TGGAGACTCA 
CCCCTGCACA TCGCAGCCAG GGAGAATCGC TACGACTGTG TTGTCCTCTT TCTTTCTCGG 
GATTCAGATG TTACTCTGAA AAACAAGGAA GGAGAGACTC CCTTGCAGTG TGCAAGTCTC 
AGTTCGCAGG TGTGGAGTGC ATTGCAGATG AGCAAAGCAC TTCGGGACTC AGCCCCTGAC 
AAGCCCGTTG CTGTTGAGAA GACGGTGAGC AGGGATATCG CTCGAGGGTA TGAGCGCATT 
CCCATTCCCT GTGTCAATGC TGTGGACAGT GAGCTGTGTC CTACCAACTA TAAGTATGTC 
TCCCAGAACT GTGTGACATC CCCCATGAAC ATTGACAGGA ACATCACTCA TTTGCAGTAC 
TGCGTGTGTG TAGATGACTG CTCCTCTAGC ACCTGCATGT GTGGCCAGCT GAGCATGCGC 
TGCTGGTATG ATAAGGATGG CCGACTTCTG CCTGAGTTTA ACATGGCAGA ACCACCCTTG 
ATCTTCGAGT GCAATCATGC CTGCTCATGC TGGAGGAACT GCCGCAATCG TGTGGTGCAA 
AATGGTCTCA GGGCAAGGCT GCAGCTTTAT CGGACACAGG ACATGGGCTG GGGTGTGCGG 
TCCCTCCAGG ATATCCCACT GGGCACCTTT GTCTGCGAAT ACGTAGGGGA GCTGATTTCG 
GACTCTGAAG CTGATGTTCG GGAAGAGGAC TCTTACCTCT TTGATCTTGA CAATAAGGAT 
GGAGAGGTAT ACTGCATTGA CGCTCGGTTC TATGGGAATG TCAGCCGGTT CATAAACCAC 
CACTGCGAAC CCAACCTTGT GCCTGTGCGA GTGTTCATGT CACACCAGGA CCTGCGGTTT 
CCCAGGATTG CCTTCTTCAG TACCCGCCTG ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC 
TACGGGGAGC GCTTTTGGGA CGTCAAGGGC AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC 
AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT 
CAGGAGAATG GCCTTCCAGA TACCAGCTCT GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108077.1
CDS	27..3800
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252217.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252217.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAAGGTAAC 
TTCATGGAAT GCCAGCCTGA GAGCAGCATC TCTCATCGTT TCCATAAGGA CTGTGCCTCT 
CGAGTCAACA ATGCCAGCTA CTGTCCCCAT TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG 
GTGACCATAG CAAAAGCAGA CACAACTTCC ACAGTGACCC TAGCCCCTGG ACAGGAGAAG 
AGCCTGGCTG CTGAAGGCAG GGCTGACACG ACCACGGGCA GCATTGCTGG AGCCCCAGAG 
GATGAAAGAT CGCAGAGCAC AGCCCCCCAG GCACCAGAGT GCTTCGACCC TGCCGGACCG 
GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT TCCCAGGGCC CAGGAAAGGA AACCTTGGAA 
AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG 
CTGTACTTCT CTGCCAGGCA GGGTGAGCTG CAGAAGGTGC TTCTCATGCT GGTTGATGGA 
ATTGATCCCA ACTTCAAAAT GGAGCACCAA AGTAAGCGTT CCCCATTACA TGCTGCTGCG 
GAGGCTGGCC ACGTGGACAT CTGCCACATG CTGGTTCAGG CGGGTGCCAA TATTGACACT 
TGCTCAGAGG ACCAGCGGAC CCCACTGATG GAGGCTGCAG AGAACAACCA CTTGGATGCA 
GTGAAGTACC TCATCAAGGC TGGAGCACAG GTGGATCCGA AGGACGCAGA GGGCTCCACA 
TGTTTGCATT TGGCTGCCAA GAAAGGCCAC TATGATGTGG TTCAGTATCT GCTTTCAAAT 
GGACAGATGG ATGTCAACTG CCAGGATGAC GGTGGATGGA CACCTATGAT CTGGGCCACT 
GAGTACAAGC ACGTGGAGCT GGTGAAGCTG CTGCTGTCTA AGGGCTCTGA CATCAACATC 
CGGGACAACG AGGAGAACAT TTGTCTGCAC TGGGCAGCAT TTTCAGGCTG TGTGGACATA 
GCTGAAATAC TTCTGGCTGC CAAGTGTGAC CTGCATGCTG TGAATATCCA TGGAGACTCA 
CCCCTGCACA TCGCAGCCAG GGAGAATCGC TACGACTGTG TTGTCCTCTT TCTTTCTCGG 
GATTCAGATG TTACTCTGAA AAACAAGGAA GGAGAGACTC CCTTGCAGTG TGCAAGTCTC 
AGTTCGCAGG TGTGGAGTGC ATTGCAGATG AGCAAAGCAC TTCGGGACTC AGCCCCTGAC 
AAGCCCGTTG CTGTTGAGAA GACGGTGAGC AGGGATATCG CTCGAGGGTA TGAGCGCATT 
CCCATTCCCT GTGTCAATGC TGTGGACAGT GAGCTGTGTC CTACCAACTA TAAGTATGTC 
TCCCAGAACT GTGTGACATC CCCCATGAAC ATTGACAGGA ACATCACTCA TTTGCAGTAC 
TGCGTGTGTG TAGATGACTG CTCCTCTAGC ACCTGCATGT GTGGCCAGCT GAGCATGCGC 
TGCTGGTATG ATAAGGATGG CCGACTTCTG CCTGAGTTTA ACATGGCAGA ACCACCCTTG 
ATCTTCGAGT GCAATCATGC CTGCTCATGC TGGAGGAACT GCCGCAATCG TGTGGTGCAA 
AATGGTCTCA GGGCAAGGCT GCAGCTTTAT CGGACACAGG ACATGGGCTG GGGTGTGCGG 
TCCCTCCAGG ATATCCCACT GGGCACCTTT GTCTGCGAAT ACGTAGGGGA GCTGATTTCG 
GACTCTGAAG CTGATGTTCG GGAAGAGGAC TCTTACCTCT TTGATCTTGA CAATAAGGAT 
GGAGAGGTAT ACTGCATTGA CGCTCGGTTC TATGGGAATG TCAGCCGGTT CATAAACCAC 
CACTGCGAAC CCAACCTTGT GCCTGTGCGA GTGTTCATGT CACACCAGGA CCTGCGGTTT 
CCCAGGATTG CCTTCTTCAG TACCCGCCTG ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC 
TACGGGGAGC GCTTTTGGGA CGTCAAGGGC AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC 
AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT 
CAGGAGAATG GCCTTCCAGA TACCAGCTCT GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252218.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108078.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X21, mRNA.

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CloneID	OMu88054
Clone ID Related Accession (Same CDS sequence)	XM_030252218.1
Accession Version	XM_030252218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAATTG GGCCGGTGTA CAAACAGCGT GGTCAAGTAT 
GAGCTGATGC GCCCATCCAA CAAAGCACCG CTCTTGGTGT TATGTGAAGA CCATCGGGGT 
CGCATGGTGA AGCACCAGTG CTGTCCTGGC TGCGGCTACT TCTGCACGGC GGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108078.1
CDS	27..3797
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252218.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252218.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAATTG GGCCGGTGTA CAAACAGCGT GGTCAAGTAT 
GAGCTGATGC GCCCATCCAA CAAAGCACCG CTCTTGGTGT TATGTGAAGA CCATCGGGGT 
CGCATGGTGA AGCACCAGTG CTGTCCTGGC TGCGGCTACT TCTGCACGGC GGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252219.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108079.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu88055
Clone ID Related Accession (Same CDS sequence)	XM_030252219.1
Accession Version	XM_030252219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108079.1
CDS	18..3788
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252219.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252219.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252220.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108080.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu88056
Clone ID Related Accession (Same CDS sequence)	XM_030252220.1
Accession Version	XM_030252220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108080.1
CDS	27..3797
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252220.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252220.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252221.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108081.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X24, mRNA.

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CloneID	OMu88057
Clone ID Related Accession (Same CDS sequence)	XM_030252221.1
Accession Version	XM_030252221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCTCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGCT ATATGGAAGT TTCCCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CAGAAAATGA AGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGCCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAAGCTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACAAC TTCCACAGTG ACCCTAGCCC CTGGACAGGA GAAGAGCCTG 
GCTGCTGAAG GCAGGGCTGA CACGACCACG GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AGATCGCAGA GCACAGCCCC CCAGGCACCA GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG 
CTCGTGAGGC CGACATCTGG CCTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTAATCGCTC TAGACTCTGA AAAACCCAAG AAACTTCGCT TCCACCCAAA GCAGCTGTAC 
TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACATGCTGC TGCGGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGC GGACCCCACT GATGGAGGCT GCAGAGAACA ACCACTTGGA TGCAGTGAAG 
TACCTCATCA AGGCTGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACATGTTTG 
CATTTGGCTG CCAAGAAAGG CCACTATGAT GTGGTTCAGT ATCTGCTTTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGACGGTGGA TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG AGCTGGTGAA GCTGCTGCTG TCTAAGGGCT CTGACATCAA CATCCGGGAC 
AACGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATACTTCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCATGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTC TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG 
CAGGTGTGGA GTGCATTGCA GATGAGCAAA GCACTTCGGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGGGAT ATCGCTCGAG GGTATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG TGTCCTACCA ACTATAAGTA TGTCTCCCAG 
AACTGTGTGA CATCCCCCAT GAACATTGAC AGGAACATCA CTCATTTGCA GTACTGCGTG 
TGTGTAGATG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGATAAGG ATGGCCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC ATGCTGGAGG AACTGCCGCA ATCGTGTGGT GCAAAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTC 
CAGGATATCC CACTGGGCAC CTTTGTCTGC GAATACGTAG GGGAGCTGAT TTCGGACTCT 
GAAGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTATACTGCA TTGACGCTCG GTTCTATGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAACCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTGCG GTTTCCCAGG 
ATTGCCTTCT TCAGTACCCG CCTGATTCAG GCTGGGGAGC AGCTCGGGTT CGACTACGGG 
GAGCGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT 
CGGCACTCAA GCGCAGCCCT GGCCCAGAGG CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCC GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108081.1
CDS	27..3794
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252221.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252221.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGGGCG AGAAAGCCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCAG AAGATGAAGA CTCCGATGAG 
CTGGAGGATG ACGAGGACCA TGGAGCTGAG CAAGCGGCTG CATTTCCCAC CGAGGATAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GACCGGGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC CGACACAGGG GAGGATGAAG ATGGTGGAGA CGAGTCTGAC 
CTGAGTTCTG AATCCAGTAT CAAGAAGAAA TTTCTCAAGA GGAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGTA GAAAGAAGCC GAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCTCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGCT ATATGGAAGT TTCCCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CAGAAAATGA AGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGCCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAAGCTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACAAC TTCCACAGTG ACCCTAGCCC CTGGACAGGA GAAGAGCCTG 
GCTGCTGAAG GCAGGGCTGA CACGACCACG GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AGATCGCAGA GCACAGCCCC CCAGGCACCA GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG 
CTCGTGAGGC CGACATCTGG CCTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTAATCGCTC TAGACTCTGA AAAACCCAAG AAACTTCGCT TCCACCCAAA GCAGCTGTAC 
TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACATGCTGC TGCGGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGC GGACCCCACT GATGGAGGCT GCAGAGAACA ACCACTTGGA TGCAGTGAAG 
TACCTCATCA AGGCTGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACATGTTTG 
CATTTGGCTG CCAAGAAAGG CCACTATGAT GTGGTTCAGT ATCTGCTTTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGACGGTGGA TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG AGCTGGTGAA GCTGCTGCTG TCTAAGGGCT CTGACATCAA CATCCGGGAC 
AACGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATACTTCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCATGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTC TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG 
CAGGTGTGGA GTGCATTGCA GATGAGCAAA GCACTTCGGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGGGAT ATCGCTCGAG GGTATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG TGTCCTACCA ACTATAAGTA TGTCTCCCAG 
AACTGTGTGA CATCCCCCAT GAACATTGAC AGGAACATCA CTCATTTGCA GTACTGCGTG 
TGTGTAGATG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGATAAGG ATGGCCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC ATGCTGGAGG AACTGCCGCA ATCGTGTGGT GCAAAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTC 
CAGGATATCC CACTGGGCAC CTTTGTCTGC GAATACGTAG GGGAGCTGAT TTCGGACTCT 
GAAGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTATACTGCA TTGACGCTCG GTTCTATGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAACCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTGCG GTTTCCCAGG 
ATTGCCTTCT TCAGTACCCG CCTGATTCAG GCTGGGGAGC AGCTCGGGTT CGACTACGGG 
GAGCGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT 
CGGCACTCAA GCGCAGCCCT GGCCCAGAGG CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCC GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252222.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108082.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X25, mRNA.

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CloneID	OMu88058
Clone ID Related Accession (Same CDS sequence)	XM_030252222.1
Accession Version	XM_030252222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAAGGTAA CTTCATGGAA 
TGCCAGCCTG AGAGCAGCAT CTCTCATCGT TTCCATAAGG ACTGTGCCTC TCGAGTCAAC 
AATGCCAGCT ACTGTCCCCA TTGTGGGGAA GAAGCTTCCA AGGCCAAAGA GGTGACCATA 
GCAAAAGCAG ACACAACTTC CACAGTGACC CTAGCCCCTG GACAGGAGAA GAGCCTGGCT 
GCTGAAGGCA GGGCTGACAC GACCACGGGC AGCATTGCTG GAGCCCCAGA GGATGAAAGA 
TCGCAGAGCA CAGCCCCCCA GGCACCAGAG TGCTTCGACC CTGCCGGACC GGCTGGGCTC 
GTGAGGCCGA CATCTGGCCT TTCCCAGGGC CCAGGAAAGG AAACCTTGGA AAGTGCTCTA 
ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA CTTCGCTTCC ACCCAAAGCA GCTGTACTTC 
TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG CTTCTCATGC TGGTTGATGG AATTGATCCC 
AACTTCAAAA TGGAGCACCA AAGTAAGCGT TCCCCATTAC ATGCTGCTGC GGAGGCTGGC 
CACGTGGACA TCTGCCACAT GCTGGTTCAG GCGGGTGCCA ATATTGACAC TTGCTCAGAG 
GACCAGCGGA CCCCACTGAT GGAGGCTGCA GAGAACAACC ACTTGGATGC AGTGAAGTAC 
CTCATCAAGG CTGGAGCACA GGTGGATCCG AAGGACGCAG AGGGCTCCAC ATGTTTGCAT 
TTGGCTGCCA AGAAAGGCCA CTATGATGTG GTTCAGTATC TGCTTTCAAA TGGACAGATG 
GATGTCAACT GCCAGGATGA CGGTGGATGG ACACCTATGA TCTGGGCCAC TGAGTACAAG 
CACGTGGAGC TGGTGAAGCT GCTGCTGTCT AAGGGCTCTG ACATCAACAT CCGGGACAAC 
GAGGAGAACA TTTGTCTGCA CTGGGCAGCA TTTTCAGGCT GTGTGGACAT AGCTGAAATA 
CTTCTGGCTG CCAAGTGTGA CCTGCATGCT GTGAATATCC ATGGAGACTC ACCCCTGCAC 
ATCGCAGCCA GGGAGAATCG CTACGACTGT GTTGTCCTCT TTCTTTCTCG GGATTCAGAT 
GTTACTCTGA AAAACAAGGA AGGAGAGACT CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG 
GTGTGGAGTG CATTGCAGAT GAGCAAAGCA CTTCGGGACT CAGCCCCTGA CAAGCCCGTT 
GCTGTTGAGA AGACGGTGAG CAGGGATATC GCTCGAGGGT ATGAGCGCAT TCCCATTCCC 
TGTGTCAATG CTGTGGACAG TGAGCTGTGT CCTACCAACT ATAAGTATGT CTCCCAGAAC 
TGTGTGACAT CCCCCATGAA CATTGACAGG AACATCACTC ATTTGCAGTA CTGCGTGTGT 
GTAGATGACT GCTCCTCTAG CACCTGCATG TGTGGCCAGC TGAGCATGCG CTGCTGGTAT 
GATAAGGATG GCCGACTTCT GCCTGAGTTT AACATGGCAG AACCACCCTT GATCTTCGAG 
TGCAATCATG CCTGCTCATG CTGGAGGAAC TGCCGCAATC GTGTGGTGCA AAATGGTCTC 
AGGGCAAGGC TGCAGCTTTA TCGGACACAG GACATGGGCT GGGGTGTGCG GTCCCTCCAG 
GATATCCCAC TGGGCACCTT TGTCTGCGAA TACGTAGGGG AGCTGATTTC GGACTCTGAA 
GCTGATGTTC GGGAAGAGGA CTCTTACCTC TTTGATCTTG ACAATAAGGA TGGAGAGGTA 
TACTGCATTG ACGCTCGGTT CTATGGGAAT GTCAGCCGGT TCATAAACCA CCACTGCGAA 
CCCAACCTTG TGCCTGTGCG AGTGTTCATG TCACACCAGG ACCTGCGGTT TCCCAGGATT 
GCCTTCTTCA GTACCCGCCT GATTCAGGCT GGGGAGCAGC TCGGGTTCGA CTACGGGGAG 
CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG 
CACTCAAGCG CAGCCCTGGC CCAGAGGCAA GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT 
GGCCTTCCAG ATACCAGCTC TGCAGCCGCT GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108082.1
CDS	18..3782
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252222.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252222.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAAGGTAA CTTCATGGAA 
TGCCAGCCTG AGAGCAGCAT CTCTCATCGT TTCCATAAGG ACTGTGCCTC TCGAGTCAAC 
AATGCCAGCT ACTGTCCCCA TTGTGGGGAA GAAGCTTCCA AGGCCAAAGA GGTGACCATA 
GCAAAAGCAG ACACAACTTC CACAGTGACC CTAGCCCCTG GACAGGAGAA GAGCCTGGCT 
GCTGAAGGCA GGGCTGACAC GACCACGGGC AGCATTGCTG GAGCCCCAGA GGATGAAAGA 
TCGCAGAGCA CAGCCCCCCA GGCACCAGAG TGCTTCGACC CTGCCGGACC GGCTGGGCTC 
GTGAGGCCGA CATCTGGCCT TTCCCAGGGC CCAGGAAAGG AAACCTTGGA AAGTGCTCTA 
ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA CTTCGCTTCC ACCCAAAGCA GCTGTACTTC 
TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG CTTCTCATGC TGGTTGATGG AATTGATCCC 
AACTTCAAAA TGGAGCACCA AAGTAAGCGT TCCCCATTAC ATGCTGCTGC GGAGGCTGGC 
CACGTGGACA TCTGCCACAT GCTGGTTCAG GCGGGTGCCA ATATTGACAC TTGCTCAGAG 
GACCAGCGGA CCCCACTGAT GGAGGCTGCA GAGAACAACC ACTTGGATGC AGTGAAGTAC 
CTCATCAAGG CTGGAGCACA GGTGGATCCG AAGGACGCAG AGGGCTCCAC ATGTTTGCAT 
TTGGCTGCCA AGAAAGGCCA CTATGATGTG GTTCAGTATC TGCTTTCAAA TGGACAGATG 
GATGTCAACT GCCAGGATGA CGGTGGATGG ACACCTATGA TCTGGGCCAC TGAGTACAAG 
CACGTGGAGC TGGTGAAGCT GCTGCTGTCT AAGGGCTCTG ACATCAACAT CCGGGACAAC 
GAGGAGAACA TTTGTCTGCA CTGGGCAGCA TTTTCAGGCT GTGTGGACAT AGCTGAAATA 
CTTCTGGCTG CCAAGTGTGA CCTGCATGCT GTGAATATCC ATGGAGACTC ACCCCTGCAC 
ATCGCAGCCA GGGAGAATCG CTACGACTGT GTTGTCCTCT TTCTTTCTCG GGATTCAGAT 
GTTACTCTGA AAAACAAGGA AGGAGAGACT CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG 
GTGTGGAGTG CATTGCAGAT GAGCAAAGCA CTTCGGGACT CAGCCCCTGA CAAGCCCGTT 
GCTGTTGAGA AGACGGTGAG CAGGGATATC GCTCGAGGGT ATGAGCGCAT TCCCATTCCC 
TGTGTCAATG CTGTGGACAG TGAGCTGTGT CCTACCAACT ATAAGTATGT CTCCCAGAAC 
TGTGTGACAT CCCCCATGAA CATTGACAGG AACATCACTC ATTTGCAGTA CTGCGTGTGT 
GTAGATGACT GCTCCTCTAG CACCTGCATG TGTGGCCAGC TGAGCATGCG CTGCTGGTAT 
GATAAGGATG GCCGACTTCT GCCTGAGTTT AACATGGCAG AACCACCCTT GATCTTCGAG 
TGCAATCATG CCTGCTCATG CTGGAGGAAC TGCCGCAATC GTGTGGTGCA AAATGGTCTC 
AGGGCAAGGC TGCAGCTTTA TCGGACACAG GACATGGGCT GGGGTGTGCG GTCCCTCCAG 
GATATCCCAC TGGGCACCTT TGTCTGCGAA TACGTAGGGG AGCTGATTTC GGACTCTGAA 
GCTGATGTTC GGGAAGAGGA CTCTTACCTC TTTGATCTTG ACAATAAGGA TGGAGAGGTA 
TACTGCATTG ACGCTCGGTT CTATGGGAAT GTCAGCCGGT TCATAAACCA CCACTGCGAA 
CCCAACCTTG TGCCTGTGCG AGTGTTCATG TCACACCAGG ACCTGCGGTT TCCCAGGATT 
GCCTTCTTCA GTACCCGCCT GATTCAGGCT GGGGAGCAGC TCGGGTTCGA CTACGGGGAG 
CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG 
CACTCAAGCG CAGCCCTGGC CCAGAGGCAA GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT 
GGCCTTCCAG ATACCAGCTC TGCAGCCGCT GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252223.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108083.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu88059
Clone ID Related Accession (Same CDS sequence)	XM_030252223.1
Accession Version	XM_030252223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAAGGTAA CTTCATGGAA 
TGCCAGCCTG AGAGCAGCAT CTCTCATCGT TTCCATAAGG ACTGTGCCTC TCGAGTCAAC 
AATGCCAGCT ACTGTCCCCA TTGTGGGGAA GAAGCTTCCA AGGCCAAAGA GGTGACCATA 
GCAAAAGCAG ACACAACTTC CACAGTGACC CTAGCCCCTG GACAGGAGAA GAGCCTGGCT 
GCTGAAGGCA GGGCTGACAC GACCACGGGC AGCATTGCTG GAGCCCCAGA GGATGAAAGA 
TCGCAGAGCA CAGCCCCCCA GGCACCAGAG TGCTTCGACC CTGCCGGACC GGCTGGGCTC 
GTGAGGCCGA CATCTGGCCT TTCCCAGGGC CCAGGAAAGG AAACCTTGGA AAGTGCTCTA 
ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA CTTCGCTTCC ACCCAAAGCA GCTGTACTTC 
TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG CTTCTCATGC TGGTTGATGG AATTGATCCC 
AACTTCAAAA TGGAGCACCA AAGTAAGCGT TCCCCATTAC ATGCTGCTGC GGAGGCTGGC 
CACGTGGACA TCTGCCACAT GCTGGTTCAG GCGGGTGCCA ATATTGACAC TTGCTCAGAG 
GACCAGCGGA CCCCACTGAT GGAGGCTGCA GAGAACAACC ACTTGGATGC AGTGAAGTAC 
CTCATCAAGG CTGGAGCACA GGTGGATCCG AAGGACGCAG AGGGCTCCAC ATGTTTGCAT 
TTGGCTGCCA AGAAAGGCCA CTATGATGTG GTTCAGTATC TGCTTTCAAA TGGACAGATG 
GATGTCAACT GCCAGGATGA CGGTGGATGG ACACCTATGA TCTGGGCCAC TGAGTACAAG 
CACGTGGAGC TGGTGAAGCT GCTGCTGTCT AAGGGCTCTG ACATCAACAT CCGGGACAAC 
GAGGAGAACA TTTGTCTGCA CTGGGCAGCA TTTTCAGGCT GTGTGGACAT AGCTGAAATA 
CTTCTGGCTG CCAAGTGTGA CCTGCATGCT GTGAATATCC ATGGAGACTC ACCCCTGCAC 
ATCGCAGCCA GGGAGAATCG CTACGACTGT GTTGTCCTCT TTCTTTCTCG GGATTCAGAT 
GTTACTCTGA AAAACAAGGA AGGAGAGACT CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG 
GTGTGGAGTG CATTGCAGAT GAGCAAAGCA CTTCGGGACT CAGCCCCTGA CAAGCCCGTT 
GCTGTTGAGA AGACGGTGAG CAGGGATATC GCTCGAGGGT ATGAGCGCAT TCCCATTCCC 
TGTGTCAATG CTGTGGACAG TGAGCTGTGT CCTACCAACT ATAAGTATGT CTCCCAGAAC 
TGTGTGACAT CCCCCATGAA CATTGACAGG AACATCACTC ATTTGCAGTA CTGCGTGTGT 
GTAGATGACT GCTCCTCTAG CACCTGCATG TGTGGCCAGC TGAGCATGCG CTGCTGGTAT 
GATAAGGATG GCCGACTTCT GCCTGAGTTT AACATGGCAG AACCACCCTT GATCTTCGAG 
TGCAATCATG CCTGCTCATG CTGGAGGAAC TGCCGCAATC GTGTGGTGCA AAATGGTCTC 
AGGGCAAGGC TGCAGCTTTA TCGGACACAG GACATGGGCT GGGGTGTGCG GTCCCTCCAG 
GATATCCCAC TGGGCACCTT TGTCTGCGAA TACGTAGGGG AGCTGATTTC GGACTCTGAA 
GCTGATGTTC GGGAAGAGGA CTCTTACCTC TTTGATCTTG ACAATAAGGA TGGAGAGGTA 
TACTGCATTG ACGCTCGGTT CTATGGGAAT GTCAGCCGGT TCATAAACCA CCACTGCGAA 
CCCAACCTTG TGCCTGTGCG AGTGTTCATG TCACACCAGG ACCTGCGGTT TCCCAGGATT 
GCCTTCTTCA GTACCCGCCT GATTCAGGCT GGGGAGCAGC TCGGGTTCGA CTACGGGGAG 
CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG 
CACTCAAGCG CAGCCCTGGC CCAGAGGCAA GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT 
GGCCTTCCAG ATACCAGCTC TGCAGCCGCT GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108083.1
CDS	27..3791
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252223.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252223.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC 
CTGCATGTAA ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG 
GAGGATGACG AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG 
ACTTCTAAAG AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG 
GAGGAACAGG AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG 
AGTTCTGAAT CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC 
TGGATCAAAC CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT 
GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC 
GCTGGCTATA TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT 
TCCCAGACAG AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC 
CAGGAAGTGC CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC 
CTGGCCAACA ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAAGGTAA CTTCATGGAA 
TGCCAGCCTG AGAGCAGCAT CTCTCATCGT TTCCATAAGG ACTGTGCCTC TCGAGTCAAC 
AATGCCAGCT ACTGTCCCCA TTGTGGGGAA GAAGCTTCCA AGGCCAAAGA GGTGACCATA 
GCAAAAGCAG ACACAACTTC CACAGTGACC CTAGCCCCTG GACAGGAGAA GAGCCTGGCT 
GCTGAAGGCA GGGCTGACAC GACCACGGGC AGCATTGCTG GAGCCCCAGA GGATGAAAGA 
TCGCAGAGCA CAGCCCCCCA GGCACCAGAG TGCTTCGACC CTGCCGGACC GGCTGGGCTC 
GTGAGGCCGA CATCTGGCCT TTCCCAGGGC CCAGGAAAGG AAACCTTGGA AAGTGCTCTA 
ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA CTTCGCTTCC ACCCAAAGCA GCTGTACTTC 
TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG CTTCTCATGC TGGTTGATGG AATTGATCCC 
AACTTCAAAA TGGAGCACCA AAGTAAGCGT TCCCCATTAC ATGCTGCTGC GGAGGCTGGC 
CACGTGGACA TCTGCCACAT GCTGGTTCAG GCGGGTGCCA ATATTGACAC TTGCTCAGAG 
GACCAGCGGA CCCCACTGAT GGAGGCTGCA GAGAACAACC ACTTGGATGC AGTGAAGTAC 
CTCATCAAGG CTGGAGCACA GGTGGATCCG AAGGACGCAG AGGGCTCCAC ATGTTTGCAT 
TTGGCTGCCA AGAAAGGCCA CTATGATGTG GTTCAGTATC TGCTTTCAAA TGGACAGATG 
GATGTCAACT GCCAGGATGA CGGTGGATGG ACACCTATGA TCTGGGCCAC TGAGTACAAG 
CACGTGGAGC TGGTGAAGCT GCTGCTGTCT AAGGGCTCTG ACATCAACAT CCGGGACAAC 
GAGGAGAACA TTTGTCTGCA CTGGGCAGCA TTTTCAGGCT GTGTGGACAT AGCTGAAATA 
CTTCTGGCTG CCAAGTGTGA CCTGCATGCT GTGAATATCC ATGGAGACTC ACCCCTGCAC 
ATCGCAGCCA GGGAGAATCG CTACGACTGT GTTGTCCTCT TTCTTTCTCG GGATTCAGAT 
GTTACTCTGA AAAACAAGGA AGGAGAGACT CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG 
GTGTGGAGTG CATTGCAGAT GAGCAAAGCA CTTCGGGACT CAGCCCCTGA CAAGCCCGTT 
GCTGTTGAGA AGACGGTGAG CAGGGATATC GCTCGAGGGT ATGAGCGCAT TCCCATTCCC 
TGTGTCAATG CTGTGGACAG TGAGCTGTGT CCTACCAACT ATAAGTATGT CTCCCAGAAC 
TGTGTGACAT CCCCCATGAA CATTGACAGG AACATCACTC ATTTGCAGTA CTGCGTGTGT 
GTAGATGACT GCTCCTCTAG CACCTGCATG TGTGGCCAGC TGAGCATGCG CTGCTGGTAT 
GATAAGGATG GCCGACTTCT GCCTGAGTTT AACATGGCAG AACCACCCTT GATCTTCGAG 
TGCAATCATG CCTGCTCATG CTGGAGGAAC TGCCGCAATC GTGTGGTGCA AAATGGTCTC 
AGGGCAAGGC TGCAGCTTTA TCGGACACAG GACATGGGCT GGGGTGTGCG GTCCCTCCAG 
GATATCCCAC TGGGCACCTT TGTCTGCGAA TACGTAGGGG AGCTGATTTC GGACTCTGAA 
GCTGATGTTC GGGAAGAGGA CTCTTACCTC TTTGATCTTG ACAATAAGGA TGGAGAGGTA 
TACTGCATTG ACGCTCGGTT CTATGGGAAT GTCAGCCGGT TCATAAACCA CCACTGCGAA 
CCCAACCTTG TGCCTGTGCG AGTGTTCATG TCACACCAGG ACCTGCGGTT TCCCAGGATT 
GCCTTCTTCA GTACCCGCCT GATTCAGGCT GGGGAGCAGC TCGGGTTCGA CTACGGGGAG 
CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG 
CACTCAAGCG CAGCCCTGGC CCAGAGGCAA GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT 
GGCCTTCCAG ATACCAGCTC TGCAGCCGCT GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252224.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108084.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu88060
Clone ID Related Accession (Same CDS sequence)	XM_030252224.1
Accession Version	XM_030252224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGATG 
GATGGAGATT CAGAGGAGGA ACAGGAGTCT CCCGACACAG GGGAGGATGA AGATGGTGGA 
GACGAGTCTG ACCTGAGTTC TGAATCCAGT ATCAAGAAGA AATTTCTCAA GAGGAGAGGG 
AAGACTGACA GCCCCTGGAT CAAACCTGCT CGGAAAAGGA GGCGGAGAAG TAGAAAGAAG 
CCGAGCAGCA TGCTTGGTTC CGAGGCTTGT AAGTCATCTC CAGGAAGCAT GGAGCAGGCA 
GCTCTGGGAG ACAGCGCTGG CTATATGGAA GTTTCCCTGG ATTCCCTGGA TCTCCGTGTC 
AGAGGAATTC TGTCTTCCCA GACAGAAAAT GAAGGGCTGG CCAGTGGTCC GGATGTGCTG 
GGGACGGATG GCCTCCAGGA AGTGCCTCTC TGCAGCTGCC GAATGGAAAC CCCCAAGAGC 
CGCGAGATCA GCACCCTGGC CAACAACCAG TGCATGGCCA CTGAGAGCGT GGATCACGAA 
TTGGGCCGGT GTACAAACAG CGTGGTCAAG TATGAGCTGA TGCGCCCATC CAACAAAGCA 
CCGCTCTTGG TGTTATGTGA AGACCATCGG GGTCGCATGG TGAAGCACCA GTGCTGTCCT 
GGCTGCGGCT ACTTCTGCAC GGCGGGTAAC TTCATGGAAT GCCAGCCTGA GAGCAGCATC 
TCTCATCGTT TCCATAAGGA CTGTGCCTCT CGAGTCAACA ATGCCAGCTA CTGTCCCCAT 
TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG GTGACCATAG CAAAAGCAGA CACAACTTCC 
ACAGTGACCC TAGCCCCTGG ACAGGAGAAG AGCCTGGCTG CTGAAGGCAG GGCTGACACG 
ACCACGGGCA GCATTGCTGG AGCCCCAGAG GATGAAAGAT CGCAGAGCAC AGCCCCCCAG 
GCACCAGAGT GCTTCGACCC TGCCGGACCG GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT 
TCCCAGGGCC CAGGAAAGGA AACCTTGGAA AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA 
CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG CTGTACTTCT CTGCCAGGCA GGGTGAGCTG 
CAGAAGGTGC TTCTCATGCT GGTTGATGGA ATTGATCCCA ACTTCAAAAT GGAGCACCAA 
AGTAAGCGTT CCCCATTACA TGCTGCTGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG 
CTGGTTCAGG CGGGTGCCAA TATTGACACT TGCTCAGAGG ACCAGCGGAC CCCACTGATG 
GAGGCTGCAG AGAACAACCA CTTGGATGCA GTGAAGTACC TCATCAAGGC TGGAGCACAG 
GTGGATCCGA AGGACGCAGA GGGCTCCACA TGTTTGCATT TGGCTGCCAA GAAAGGCCAC 
TATGATGTGG TTCAGTATCT GCTTTCAAAT GGACAGATGG ATGTCAACTG CCAGGATGAC 
GGTGGATGGA CACCTATGAT CTGGGCCACT GAGTACAAGC ACGTGGAGCT GGTGAAGCTG 
CTGCTGTCTA AGGGCTCTGA CATCAACATC CGGGACAACG AGGAGAACAT TTGTCTGCAC 
TGGGCAGCAT TTTCAGGCTG TGTGGACATA GCTGAAATAC TTCTGGCTGC CAAGTGTGAC 
CTGCATGCTG TGAATATCCA TGGAGACTCA CCCCTGCACA TCGCAGCCAG GGAGAATCGC 
TACGACTGTG TTGTCCTCTT TCTTTCTCGG GATTCAGATG TTACTCTGAA AAACAAGGAA 
GGAGAGACTC CCTTGCAGTG TGCAAGTCTC AGTTCGCAGG TGTGGAGTGC ATTGCAGATG 
AGCAAAGCAC TTCGGGACTC AGCCCCTGAC AAGCCCGTTG CTGTTGAGAA GACGGTGAGC 
AGGGATATCG CTCGAGGGTA TGAGCGCATT CCCATTCCCT GTGTCAATGC TGTGGACAGT 
GAGCTGTGTC CTACCAACTA TAAGTATGTC TCCCAGAACT GTGTGACATC CCCCATGAAC 
ATTGACAGGA ACATCACTCA TTTGCAGTAC TGCGTGTGTG TAGATGACTG CTCCTCTAGC 
ACCTGCATGT GTGGCCAGCT GAGCATGCGC TGCTGGTATG ATAAGGATGG CCGACTTCTG 
CCTGAGTTTA ACATGGCAGA ACCACCCTTG ATCTTCGAGT GCAATCATGC CTGCTCATGC 
TGGAGGAACT GCCGCAATCG TGTGGTGCAA AATGGTCTCA GGGCAAGGCT GCAGCTTTAT 
CGGACACAGG ACATGGGCTG GGGTGTGCGG TCCCTCCAGG ATATCCCACT GGGCACCTTT 
GTCTGCGAAT ACGTAGGGGA GCTGATTTCG GACTCTGAAG CTGATGTTCG GGAAGAGGAC 
TCTTACCTCT TTGATCTTGA CAATAAGGAT GGAGAGGTAT ACTGCATTGA CGCTCGGTTC 
TATGGGAATG TCAGCCGGTT CATAAACCAC CACTGCGAAC CCAACCTTGT GCCTGTGCGA 
GTGTTCATGT CACACCAGGA CCTGCGGTTT CCCAGGATTG CCTTCTTCAG TACCCGCCTG 
ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC TACGGGGAGC GCTTTTGGGA CGTCAAGGGC 
AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC 
CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT CAGGAGAATG GCCTTCCAGA TACCAGCTCT 
GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108084.1
CDS	27..3770
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252224.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252224.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGATG 
GATGGAGATT CAGAGGAGGA ACAGGAGTCT CCCGACACAG GGGAGGATGA AGATGGTGGA 
GACGAGTCTG ACCTGAGTTC TGAATCCAGT ATCAAGAAGA AATTTCTCAA GAGGAGAGGG 
AAGACTGACA GCCCCTGGAT CAAACCTGCT CGGAAAAGGA GGCGGAGAAG TAGAAAGAAG 
CCGAGCAGCA TGCTTGGTTC CGAGGCTTGT AAGTCATCTC CAGGAAGCAT GGAGCAGGCA 
GCTCTGGGAG ACAGCGCTGG CTATATGGAA GTTTCCCTGG ATTCCCTGGA TCTCCGTGTC 
AGAGGAATTC TGTCTTCCCA GACAGAAAAT GAAGGGCTGG CCAGTGGTCC GGATGTGCTG 
GGGACGGATG GCCTCCAGGA AGTGCCTCTC TGCAGCTGCC GAATGGAAAC CCCCAAGAGC 
CGCGAGATCA GCACCCTGGC CAACAACCAG TGCATGGCCA CTGAGAGCGT GGATCACGAA 
TTGGGCCGGT GTACAAACAG CGTGGTCAAG TATGAGCTGA TGCGCCCATC CAACAAAGCA 
CCGCTCTTGG TGTTATGTGA AGACCATCGG GGTCGCATGG TGAAGCACCA GTGCTGTCCT 
GGCTGCGGCT ACTTCTGCAC GGCGGGTAAC TTCATGGAAT GCCAGCCTGA GAGCAGCATC 
TCTCATCGTT TCCATAAGGA CTGTGCCTCT CGAGTCAACA ATGCCAGCTA CTGTCCCCAT 
TGTGGGGAAG AAGCTTCCAA GGCCAAAGAG GTGACCATAG CAAAAGCAGA CACAACTTCC 
ACAGTGACCC TAGCCCCTGG ACAGGAGAAG AGCCTGGCTG CTGAAGGCAG GGCTGACACG 
ACCACGGGCA GCATTGCTGG AGCCCCAGAG GATGAAAGAT CGCAGAGCAC AGCCCCCCAG 
GCACCAGAGT GCTTCGACCC TGCCGGACCG GCTGGGCTCG TGAGGCCGAC ATCTGGCCTT 
TCCCAGGGCC CAGGAAAGGA AACCTTGGAA AGTGCTCTAA TCGCTCTAGA CTCTGAAAAA 
CCCAAGAAAC TTCGCTTCCA CCCAAAGCAG CTGTACTTCT CTGCCAGGCA GGGTGAGCTG 
CAGAAGGTGC TTCTCATGCT GGTTGATGGA ATTGATCCCA ACTTCAAAAT GGAGCACCAA 
AGTAAGCGTT CCCCATTACA TGCTGCTGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG 
CTGGTTCAGG CGGGTGCCAA TATTGACACT TGCTCAGAGG ACCAGCGGAC CCCACTGATG 
GAGGCTGCAG AGAACAACCA CTTGGATGCA GTGAAGTACC TCATCAAGGC TGGAGCACAG 
GTGGATCCGA AGGACGCAGA GGGCTCCACA TGTTTGCATT TGGCTGCCAA GAAAGGCCAC 
TATGATGTGG TTCAGTATCT GCTTTCAAAT GGACAGATGG ATGTCAACTG CCAGGATGAC 
GGTGGATGGA CACCTATGAT CTGGGCCACT GAGTACAAGC ACGTGGAGCT GGTGAAGCTG 
CTGCTGTCTA AGGGCTCTGA CATCAACATC CGGGACAACG AGGAGAACAT TTGTCTGCAC 
TGGGCAGCAT TTTCAGGCTG TGTGGACATA GCTGAAATAC TTCTGGCTGC CAAGTGTGAC 
CTGCATGCTG TGAATATCCA TGGAGACTCA CCCCTGCACA TCGCAGCCAG GGAGAATCGC 
TACGACTGTG TTGTCCTCTT TCTTTCTCGG GATTCAGATG TTACTCTGAA AAACAAGGAA 
GGAGAGACTC CCTTGCAGTG TGCAAGTCTC AGTTCGCAGG TGTGGAGTGC ATTGCAGATG 
AGCAAAGCAC TTCGGGACTC AGCCCCTGAC AAGCCCGTTG CTGTTGAGAA GACGGTGAGC 
AGGGATATCG CTCGAGGGTA TGAGCGCATT CCCATTCCCT GTGTCAATGC TGTGGACAGT 
GAGCTGTGTC CTACCAACTA TAAGTATGTC TCCCAGAACT GTGTGACATC CCCCATGAAC 
ATTGACAGGA ACATCACTCA TTTGCAGTAC TGCGTGTGTG TAGATGACTG CTCCTCTAGC 
ACCTGCATGT GTGGCCAGCT GAGCATGCGC TGCTGGTATG ATAAGGATGG CCGACTTCTG 
CCTGAGTTTA ACATGGCAGA ACCACCCTTG ATCTTCGAGT GCAATCATGC CTGCTCATGC 
TGGAGGAACT GCCGCAATCG TGTGGTGCAA AATGGTCTCA GGGCAAGGCT GCAGCTTTAT 
CGGACACAGG ACATGGGCTG GGGTGTGCGG TCCCTCCAGG ATATCCCACT GGGCACCTTT 
GTCTGCGAAT ACGTAGGGGA GCTGATTTCG GACTCTGAAG CTGATGTTCG GGAAGAGGAC 
TCTTACCTCT TTGATCTTGA CAATAAGGAT GGAGAGGTAT ACTGCATTGA CGCTCGGTTC 
TATGGGAATG TCAGCCGGTT CATAAACCAC CACTGCGAAC CCAACCTTGT GCCTGTGCGA 
GTGTTCATGT CACACCAGGA CCTGCGGTTT CCCAGGATTG CCTTCTTCAG TACCCGCCTG 
ATTCAGGCTG GGGAGCAGCT CGGGTTCGAC TACGGGGAGC GCTTTTGGGA CGTCAAGGGC 
AAGCTCTTCA GTTGCCGGTG TGGGTCTTCC AAGTGTCGGC ACTCAAGCGC AGCCCTGGCC 
CAGAGGCAAG CCAGTGCAGC CCAGGAGCCT CAGGAGAATG GCCTTCCAGA TACCAGCTCT 
GCAGCCGCTG CTGACCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252225.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108085.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu88061
Clone ID Related Accession (Same CDS sequence)	XM_030252225.1
Accession Version	XM_030252225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGA TGGATGGAGA TTCAGAGGAG 
GAACAGGAGT CTCCCGACAC AGGGGAGGAT GAAGATGGTG GAGACGAGTC TGACCTGAGT 
TCTGAATCCA GTATCAAGAA GAAATTTCTC AAGAGGAGAG GGAAGACTGA CAGCCCCTGG 
ATCAAACCTG CTCGGAAAAG GAGGCGGAGA AGTAGAAAGA AGCCGAGCAG CATGCTTGGT 
TCCGAGGCTT GTAAGTCATC TCCAGGAAGC ATGGAGCAGG CAGCTCTGGG AGACAGCGCT 
GGCTATATGG AAGTTTCCCT GGATTCCCTG GATCTCCGTG TCAGAGGAAT TCTGTCTTCC 
CAGACAGAAA ATGAAGGGCT GGCCAGTGGT CCGGATGTGC TGGGGACGGA TGGCCTCCAG 
GAAGTGCCTC TCTGCAGCTG CCGAATGGAA ACCCCCAAGA GCCGCGAGAT CAGCACCCTG 
GCCAACAACC AGTGCATGGC CACTGAGAGC GTGGATCACG AATTGGGCCG GTGTACAAAC 
AGCGTGGTCA AGTATGAGCT GATGCGCCCA TCCAACAAAG CACCGCTCTT GGTGTTATGT 
GAAGACCATC GGGGTCGCAT GGTGAAGCAC CAGTGCTGTC CTGGCTGCGG CTACTTCTGC 
ACGGCGGGTA ACTTCATGGA ATGCCAGCCT GAGAGCAGCA TCTCTCATCG TTTCCATAAG 
GACTGTGCCT CTCGAGTCAA CAATGCCAGC TACTGTCCCC ATTGTGGGGA AGAAGCTTCC 
AAGGCCAAAG AGGTGACCAT AGCAAAAGCA GACACAACTT CCACAGTGAC CCTAGCCCCT 
GGACAGGAGA AGAGCCTGGC TGCTGAAGGC AGGGCTGACA CGACCACGGG CAGCATTGCT 
GGAGCCCCAG AGGATGAAAG ATCGCAGAGC ACAGCCCCCC AGGCACCAGA GTGCTTCGAC 
CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG ACATCTGGCC TTTCCCAGGG CCCAGGAAAG 
GAAACCTTGG AAAGTGCTCT AATCGCTCTA GACTCTGAAA AACCCAAGAA ACTTCGCTTC 
CACCCAAAGC AGCTGTACTT CTCTGCCAGG CAGGGTGAGC TGCAGAAGGT GCTTCTCATG 
CTGGTTGATG GAATTGATCC CAACTTCAAA ATGGAGCACC AAAGTAAGCG TTCCCCATTA 
CATGCTGCTG CGGAGGCTGG CCACGTGGAC ATCTGCCACA TGCTGGTTCA GGCGGGTGCC 
AATATTGACA CTTGCTCAGA GGACCAGCGG ACCCCACTGA TGGAGGCTGC AGAGAACAAC 
CACTTGGATG CAGTGAAGTA CCTCATCAAG GCTGGAGCAC AGGTGGATCC GAAGGACGCA 
GAGGGCTCCA CATGTTTGCA TTTGGCTGCC AAGAAAGGCC ACTATGATGT GGTTCAGTAT 
CTGCTTTCAA ATGGACAGAT GGATGTCAAC TGCCAGGATG ACGGTGGATG GACACCTATG 
ATCTGGGCCA CTGAGTACAA GCACGTGGAG CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT 
GACATCAACA TCCGGGACAA CGAGGAGAAC ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC 
TGTGTGGACA TAGCTGAAAT ACTTCTGGCT GCCAAGTGTG ACCTGCATGC TGTGAATATC 
CATGGAGACT CACCCCTGCA CATCGCAGCC AGGGAGAATC GCTACGACTG TGTTGTCCTC 
TTTCTTTCTC GGGATTCAGA TGTTACTCTG AAAAACAAGG AAGGAGAGAC TCCCTTGCAG 
TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT GCATTGCAGA TGAGCAAAGC ACTTCGGGAC 
TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG AAGACGGTGA GCAGGGATAT CGCTCGAGGG 
TATGAGCGCA TTCCCATTCC CTGTGTCAAT GCTGTGGACA GTGAGCTGTG TCCTACCAAC 
TATAAGTATG TCTCCCAGAA CTGTGTGACA TCCCCCATGA ACATTGACAG GAACATCACT 
CATTTGCAGT ACTGCGTGTG TGTAGATGAC TGCTCCTCTA GCACCTGCAT GTGTGGCCAG 
CTGAGCATGC GCTGCTGGTA TGATAAGGAT GGCCGACTTC TGCCTGAGTT TAACATGGCA 
GAACCACCCT TGATCTTCGA GTGCAATCAT GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT 
CGTGTGGTGC AAAATGGTCT CAGGGCAAGG CTGCAGCTTT ATCGGACACA GGACATGGGC 
TGGGGTGTGC GGTCCCTCCA GGATATCCCA CTGGGCACCT TTGTCTGCGA ATACGTAGGG 
GAGCTGATTT CGGACTCTGA AGCTGATGTT CGGGAAGAGG ACTCTTACCT CTTTGATCTT 
GACAATAAGG ATGGAGAGGT ATACTGCATT GACGCTCGGT TCTATGGGAA TGTCAGCCGG 
TTCATAAACC ACCACTGCGA ACCCAACCTT GTGCCTGTGC GAGTGTTCAT GTCACACCAG 
GACCTGCGGT TTCCCAGGAT TGCCTTCTTC AGTACCCGCC TGATTCAGGC TGGGGAGCAG 
CTCGGGTTCG ACTACGGGGA GCGCTTTTGG GACGTCAAGG GCAAGCTCTT CAGTTGCCGG 
TGTGGGTCTT CCAAGTGTCG GCACTCAAGC GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA 
GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA GATACCAGCT CTGCAGCCGC TGCTGACCCC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108085.1
CDS	18..3743
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252225.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252225.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGA TGGATGGAGA TTCAGAGGAG 
GAACAGGAGT CTCCCGACAC AGGGGAGGAT GAAGATGGTG GAGACGAGTC TGACCTGAGT 
TCTGAATCCA GTATCAAGAA GAAATTTCTC AAGAGGAGAG GGAAGACTGA CAGCCCCTGG 
ATCAAACCTG CTCGGAAAAG GAGGCGGAGA AGTAGAAAGA AGCCGAGCAG CATGCTTGGT 
TCCGAGGCTT GTAAGTCATC TCCAGGAAGC ATGGAGCAGG CAGCTCTGGG AGACAGCGCT 
GGCTATATGG AAGTTTCCCT GGATTCCCTG GATCTCCGTG TCAGAGGAAT TCTGTCTTCC 
CAGACAGAAA ATGAAGGGCT GGCCAGTGGT CCGGATGTGC TGGGGACGGA TGGCCTCCAG 
GAAGTGCCTC TCTGCAGCTG CCGAATGGAA ACCCCCAAGA GCCGCGAGAT CAGCACCCTG 
GCCAACAACC AGTGCATGGC CACTGAGAGC GTGGATCACG AATTGGGCCG GTGTACAAAC 
AGCGTGGTCA AGTATGAGCT GATGCGCCCA TCCAACAAAG CACCGCTCTT GGTGTTATGT 
GAAGACCATC GGGGTCGCAT GGTGAAGCAC CAGTGCTGTC CTGGCTGCGG CTACTTCTGC 
ACGGCGGGTA ACTTCATGGA ATGCCAGCCT GAGAGCAGCA TCTCTCATCG TTTCCATAAG 
GACTGTGCCT CTCGAGTCAA CAATGCCAGC TACTGTCCCC ATTGTGGGGA AGAAGCTTCC 
AAGGCCAAAG AGGTGACCAT AGCAAAAGCA GACACAACTT CCACAGTGAC CCTAGCCCCT 
GGACAGGAGA AGAGCCTGGC TGCTGAAGGC AGGGCTGACA CGACCACGGG CAGCATTGCT 
GGAGCCCCAG AGGATGAAAG ATCGCAGAGC ACAGCCCCCC AGGCACCAGA GTGCTTCGAC 
CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG ACATCTGGCC TTTCCCAGGG CCCAGGAAAG 
GAAACCTTGG AAAGTGCTCT AATCGCTCTA GACTCTGAAA AACCCAAGAA ACTTCGCTTC 
CACCCAAAGC AGCTGTACTT CTCTGCCAGG CAGGGTGAGC TGCAGAAGGT GCTTCTCATG 
CTGGTTGATG GAATTGATCC CAACTTCAAA ATGGAGCACC AAAGTAAGCG TTCCCCATTA 
CATGCTGCTG CGGAGGCTGG CCACGTGGAC ATCTGCCACA TGCTGGTTCA GGCGGGTGCC 
AATATTGACA CTTGCTCAGA GGACCAGCGG ACCCCACTGA TGGAGGCTGC AGAGAACAAC 
CACTTGGATG CAGTGAAGTA CCTCATCAAG GCTGGAGCAC AGGTGGATCC GAAGGACGCA 
GAGGGCTCCA CATGTTTGCA TTTGGCTGCC AAGAAAGGCC ACTATGATGT GGTTCAGTAT 
CTGCTTTCAA ATGGACAGAT GGATGTCAAC TGCCAGGATG ACGGTGGATG GACACCTATG 
ATCTGGGCCA CTGAGTACAA GCACGTGGAG CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT 
GACATCAACA TCCGGGACAA CGAGGAGAAC ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC 
TGTGTGGACA TAGCTGAAAT ACTTCTGGCT GCCAAGTGTG ACCTGCATGC TGTGAATATC 
CATGGAGACT CACCCCTGCA CATCGCAGCC AGGGAGAATC GCTACGACTG TGTTGTCCTC 
TTTCTTTCTC GGGATTCAGA TGTTACTCTG AAAAACAAGG AAGGAGAGAC TCCCTTGCAG 
TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT GCATTGCAGA TGAGCAAAGC ACTTCGGGAC 
TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG AAGACGGTGA GCAGGGATAT CGCTCGAGGG 
TATGAGCGCA TTCCCATTCC CTGTGTCAAT GCTGTGGACA GTGAGCTGTG TCCTACCAAC 
TATAAGTATG TCTCCCAGAA CTGTGTGACA TCCCCCATGA ACATTGACAG GAACATCACT 
CATTTGCAGT ACTGCGTGTG TGTAGATGAC TGCTCCTCTA GCACCTGCAT GTGTGGCCAG 
CTGAGCATGC GCTGCTGGTA TGATAAGGAT GGCCGACTTC TGCCTGAGTT TAACATGGCA 
GAACCACCCT TGATCTTCGA GTGCAATCAT GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT 
CGTGTGGTGC AAAATGGTCT CAGGGCAAGG CTGCAGCTTT ATCGGACACA GGACATGGGC 
TGGGGTGTGC GGTCCCTCCA GGATATCCCA CTGGGCACCT TTGTCTGCGA ATACGTAGGG 
GAGCTGATTT CGGACTCTGA AGCTGATGTT CGGGAAGAGG ACTCTTACCT CTTTGATCTT 
GACAATAAGG ATGGAGAGGT ATACTGCATT GACGCTCGGT TCTATGGGAA TGTCAGCCGG 
TTCATAAACC ACCACTGCGA ACCCAACCTT GTGCCTGTGC GAGTGTTCAT GTCACACCAG 
GACCTGCGGT TTCCCAGGAT TGCCTTCTTC AGTACCCGCC TGATTCAGGC TGGGGAGCAG 
CTCGGGTTCG ACTACGGGGA GCGCTTTTGG GACGTCAAGG GCAAGCTCTT CAGTTGCCGG 
TGTGGGTCTT CCAAGTGTCG GCACTCAAGC GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA 
GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA GATACCAGCT CTGCAGCCGC TGCTGACCCC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252226.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108086.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X30, mRNA.

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CloneID	OMu88062
Clone ID Related Accession (Same CDS sequence)	XM_030252226.1
Accession Version	XM_030252226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGATGG ATGGAGATTC AGAGGAGGAA 
CAGGAGTCTC CCGACACAGG GGAGGATGAA GATGGTGGAG ACGAGTCTGA CCTGAGTTCT 
GAATCCAGTA TCAAGAAGAA ATTTCTCAAG AGGAGAGGGA AGACTGACAG CCCCTGGATC 
AAACCTGCTC GGAAAAGGAG GCGGAGAAGT AGAAAGAAGC CGAGCAGCAT GCTTGGTTCC 
GAGGCTTGTA AGTCATCTCC AGGAAGCATG GAGCAGGCAG CTCTGGGAGA CAGCGCTGGC 
TATATGGAAG TTTCCCTGGA TTCCCTGGAT CTCCGTGTCA GAGGAATTCT GTCTTCCCAG 
ACAGAAAATG AAGGGCTGGC CAGTGGTCCG GATGTGCTGG GGACGGATGG CCTCCAGGAA 
GTGCCTCTCT GCAGCTGCCG AATGGAAACC CCCAAGAGCC GCGAGATCAG CACCCTGGCC 
AACAACCAGT GCATGGCCAC TGAGAGCGTG GATCACGAAT TGGGCCGGTG TACAAACAGC 
GTGGTCAAGT ATGAGCTGAT GCGCCCATCC AACAAAGCAC CGCTCTTGGT GTTATGTGAA 
GACCATCGGG GTCGCATGGT GAAGCACCAG TGCTGTCCTG GCTGCGGCTA CTTCTGCACG 
GCGGGTAACT TCATGGAATG CCAGCCTGAG AGCAGCATCT CTCATCGTTT CCATAAGGAC 
TGTGCCTCTC GAGTCAACAA TGCCAGCTAC TGTCCCCATT GTGGGGAAGA AGCTTCCAAG 
GCCAAAGAGG TGACCATAGC AAAAGCAGAC ACAACTTCCA CAGTGACCCT AGCCCCTGGA 
CAGGAGAAGA GCCTGGCTGC TGAAGGCAGG GCTGACACGA CCACGGGCAG CATTGCTGGA 
GCCCCAGAGG ATGAAAGATC GCAGAGCACA GCCCCCCAGG CACCAGAGTG CTTCGACCCT 
GCCGGACCGG CTGGGCTCGT GAGGCCGACA TCTGGCCTTT CCCAGGGCCC AGGAAAGGAA 
ACCTTGGAAA GTGCTCTAAT CGCTCTAGAC TCTGAAAAAC CCAAGAAACT TCGCTTCCAC 
CCAAAGCAGC TGTACTTCTC TGCCAGGCAG GGTGAGCTGC AGAAGGTGCT TCTCATGCTG 
GTTGATGGAA TTGATCCCAA CTTCAAAATG GAGCACCAAA GTAAGCGTTC CCCATTACAT 
GCTGCTGCGG AGGCTGGCCA CGTGGACATC TGCCACATGC TGGTTCAGGC GGGTGCCAAT 
ATTGACACTT GCTCAGAGGA CCAGCGGACC CCACTGATGG AGGCTGCAGA GAACAACCAC 
TTGGATGCAG TGAAGTACCT CATCAAGGCT GGAGCACAGG TGGATCCGAA GGACGCAGAG 
GGCTCCACAT GTTTGCATTT GGCTGCCAAG AAAGGCCACT ATGATGTGGT TCAGTATCTG 
CTTTCAAATG GACAGATGGA TGTCAACTGC CAGGATGACG GTGGATGGAC ACCTATGATC 
TGGGCCACTG AGTACAAGCA CGTGGAGCTG GTGAAGCTGC TGCTGTCTAA GGGCTCTGAC 
ATCAACATCC GGGACAACGA GGAGAACATT TGTCTGCACT GGGCAGCATT TTCAGGCTGT 
GTGGACATAG CTGAAATACT TCTGGCTGCC AAGTGTGACC TGCATGCTGT GAATATCCAT 
GGAGACTCAC CCCTGCACAT CGCAGCCAGG GAGAATCGCT ACGACTGTGT TGTCCTCTTT 
CTTTCTCGGG ATTCAGATGT TACTCTGAAA AACAAGGAAG GAGAGACTCC CTTGCAGTGT 
GCAAGTCTCA GTTCGCAGGT GTGGAGTGCA TTGCAGATGA GCAAAGCACT TCGGGACTCA 
GCCCCTGACA AGCCCGTTGC TGTTGAGAAG ACGGTGAGCA GGGATATCGC TCGAGGGTAT 
GAGCGCATTC CCATTCCCTG TGTCAATGCT GTGGACAGTG AGCTGTGTCC TACCAACTAT 
AAGTATGTCT CCCAGAACTG TGTGACATCC CCCATGAACA TTGACAGGAA CATCACTCAT 
TTGCAGTACT GCGTGTGTGT AGATGACTGC TCCTCTAGCA CCTGCATGTG TGGCCAGCTG 
AGCATGCGCT GCTGGTATGA TAAGGATGGC CGACTTCTGC CTGAGTTTAA CATGGCAGAA 
CCACCCTTGA TCTTCGAGTG CAATCATGCC TGCTCATGCT GGAGGAACTG CCGCAATCGT 
GTGGTGCAAA ATGGTCTCAG GGCAAGGCTG CAGCTTTATC GGACACAGGA CATGGGCTGG 
GGTGTGCGGT CCCTCCAGGA TATCCCACTG GGCACCTTTG TCTGCGAATA CGTAGGGGAG 
CTGATTTCGG ACTCTGAAGC TGATGTTCGG GAAGAGGACT CTTACCTCTT TGATCTTGAC 
AATAAGGATG GAGAGGTATA CTGCATTGAC GCTCGGTTCT ATGGGAATGT CAGCCGGTTC 
ATAAACCACC ACTGCGAACC CAACCTTGTG CCTGTGCGAG TGTTCATGTC ACACCAGGAC 
CTGCGGTTTC CCAGGATTGC CTTCTTCAGT ACCCGCCTGA TTCAGGCTGG GGAGCAGCTC 
GGGTTCGACT ACGGGGAGCG CTTTTGGGAC GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT 
GGGTCTTCCA AGTGTCGGCA CTCAAGCGCA GCCCTGGCCC AGAGGCAAGC CAGTGCAGCC 
CAGGAGCCTC AGGAGAATGG CCTTCCAGAT ACCAGCTCTG CAGCCGCTGC TGACCCCCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108086.1
CDS	27..3749
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252226.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252226.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTA TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGTTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAAC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TAACCCATTC CACTGTGGGC GCCAAGATGG ATGGAGATTC AGAGGAGGAA 
CAGGAGTCTC CCGACACAGG GGAGGATGAA GATGGTGGAG ACGAGTCTGA CCTGAGTTCT 
GAATCCAGTA TCAAGAAGAA ATTTCTCAAG AGGAGAGGGA AGACTGACAG CCCCTGGATC 
AAACCTGCTC GGAAAAGGAG GCGGAGAAGT AGAAAGAAGC CGAGCAGCAT GCTTGGTTCC 
GAGGCTTGTA AGTCATCTCC AGGAAGCATG GAGCAGGCAG CTCTGGGAGA CAGCGCTGGC 
TATATGGAAG TTTCCCTGGA TTCCCTGGAT CTCCGTGTCA GAGGAATTCT GTCTTCCCAG 
ACAGAAAATG AAGGGCTGGC CAGTGGTCCG GATGTGCTGG GGACGGATGG CCTCCAGGAA 
GTGCCTCTCT GCAGCTGCCG AATGGAAACC CCCAAGAGCC GCGAGATCAG CACCCTGGCC 
AACAACCAGT GCATGGCCAC TGAGAGCGTG GATCACGAAT TGGGCCGGTG TACAAACAGC 
GTGGTCAAGT ATGAGCTGAT GCGCCCATCC AACAAAGCAC CGCTCTTGGT GTTATGTGAA 
GACCATCGGG GTCGCATGGT GAAGCACCAG TGCTGTCCTG GCTGCGGCTA CTTCTGCACG 
GCGGGTAACT TCATGGAATG CCAGCCTGAG AGCAGCATCT CTCATCGTTT CCATAAGGAC 
TGTGCCTCTC GAGTCAACAA TGCCAGCTAC TGTCCCCATT GTGGGGAAGA AGCTTCCAAG 
GCCAAAGAGG TGACCATAGC AAAAGCAGAC ACAACTTCCA CAGTGACCCT AGCCCCTGGA 
CAGGAGAAGA GCCTGGCTGC TGAAGGCAGG GCTGACACGA CCACGGGCAG CATTGCTGGA 
GCCCCAGAGG ATGAAAGATC GCAGAGCACA GCCCCCCAGG CACCAGAGTG CTTCGACCCT 
GCCGGACCGG CTGGGCTCGT GAGGCCGACA TCTGGCCTTT CCCAGGGCCC AGGAAAGGAA 
ACCTTGGAAA GTGCTCTAAT CGCTCTAGAC TCTGAAAAAC CCAAGAAACT TCGCTTCCAC 
CCAAAGCAGC TGTACTTCTC TGCCAGGCAG GGTGAGCTGC AGAAGGTGCT TCTCATGCTG 
GTTGATGGAA TTGATCCCAA CTTCAAAATG GAGCACCAAA GTAAGCGTTC CCCATTACAT 
GCTGCTGCGG AGGCTGGCCA CGTGGACATC TGCCACATGC TGGTTCAGGC GGGTGCCAAT 
ATTGACACTT GCTCAGAGGA CCAGCGGACC CCACTGATGG AGGCTGCAGA GAACAACCAC 
TTGGATGCAG TGAAGTACCT CATCAAGGCT GGAGCACAGG TGGATCCGAA GGACGCAGAG 
GGCTCCACAT GTTTGCATTT GGCTGCCAAG AAAGGCCACT ATGATGTGGT TCAGTATCTG 
CTTTCAAATG GACAGATGGA TGTCAACTGC CAGGATGACG GTGGATGGAC ACCTATGATC 
TGGGCCACTG AGTACAAGCA CGTGGAGCTG GTGAAGCTGC TGCTGTCTAA GGGCTCTGAC 
ATCAACATCC GGGACAACGA GGAGAACATT TGTCTGCACT GGGCAGCATT TTCAGGCTGT 
GTGGACATAG CTGAAATACT TCTGGCTGCC AAGTGTGACC TGCATGCTGT GAATATCCAT 
GGAGACTCAC CCCTGCACAT CGCAGCCAGG GAGAATCGCT ACGACTGTGT TGTCCTCTTT 
CTTTCTCGGG ATTCAGATGT TACTCTGAAA AACAAGGAAG GAGAGACTCC CTTGCAGTGT 
GCAAGTCTCA GTTCGCAGGT GTGGAGTGCA TTGCAGATGA GCAAAGCACT TCGGGACTCA 
GCCCCTGACA AGCCCGTTGC TGTTGAGAAG ACGGTGAGCA GGGATATCGC TCGAGGGTAT 
GAGCGCATTC CCATTCCCTG TGTCAATGCT GTGGACAGTG AGCTGTGTCC TACCAACTAT 
AAGTATGTCT CCCAGAACTG TGTGACATCC CCCATGAACA TTGACAGGAA CATCACTCAT 
TTGCAGTACT GCGTGTGTGT AGATGACTGC TCCTCTAGCA CCTGCATGTG TGGCCAGCTG 
AGCATGCGCT GCTGGTATGA TAAGGATGGC CGACTTCTGC CTGAGTTTAA CATGGCAGAA 
CCACCCTTGA TCTTCGAGTG CAATCATGCC TGCTCATGCT GGAGGAACTG CCGCAATCGT 
GTGGTGCAAA ATGGTCTCAG GGCAAGGCTG CAGCTTTATC GGACACAGGA CATGGGCTGG 
GGTGTGCGGT CCCTCCAGGA TATCCCACTG GGCACCTTTG TCTGCGAATA CGTAGGGGAG 
CTGATTTCGG ACTCTGAAGC TGATGTTCGG GAAGAGGACT CTTACCTCTT TGATCTTGAC 
AATAAGGATG GAGAGGTATA CTGCATTGAC GCTCGGTTCT ATGGGAATGT CAGCCGGTTC 
ATAAACCACC ACTGCGAACC CAACCTTGTG CCTGTGCGAG TGTTCATGTC ACACCAGGAC 
CTGCGGTTTC CCAGGATTGC CTTCTTCAGT ACCCGCCTGA TTCAGGCTGG GGAGCAGCTC 
GGGTTCGACT ACGGGGAGCG CTTTTGGGAC GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT 
GGGTCTTCCA AGTGTCGGCA CTCAAGCGCA GCCCTGGCCC AGAGGCAAGC CAGTGCAGCC 
CAGGAGCCTC AGGAGAATGG CCTTCCAGAT ACCAGCTCTG CAGCCGCTGC TGACCCCCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252227.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108087.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OMu88063
Clone ID Related Accession (Same CDS sequence)	XM_030252227.1
Accession Version	XM_030252227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGATGGATG GAGATTCAGA GGAGGAACAG 
GAGTCTCCCG ACACAGGGGA GGATGAAGAT GGTGGAGACG AGTCTGACCT GAGTTCTGAA 
TCCAGTATCA AGAAGAAATT TCTCAAGAGG AGAGGGAAGA CTGACAGCCC CTGGATCAAA 
CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA AAGAAGCCGA GCAGCATGCT TGGTTCCGAG 
GCTTGTAAGT CATCTCCAGG AAGCATGGAG CAGGCAGCTC TGGGAGACAG CGCTGGCTAT 
ATGGAAGTTT CCCTGGATTC CCTGGATCTC CGTGTCAGAG GAATTCTGTC TTCCCAGACA 
GAAAATGAAG GGCTGGCCAG TGGTCCGGAT GTGCTGGGGA CGGATGGCCT CCAGGAAGTG 
CCTCTCTGCA GCTGCCGAAT GGAAACCCCC AAGAGCCGCG AGATCAGCAC CCTGGCCAAC 
AACCAGTGCA TGGCCACTGA GAGCGTGGAT CACGAATTGG GCCGGTGTAC AAACAGCGTG 
GTCAAGTATG AGCTGATGCG CCCATCCAAC AAAGCACCGC TCTTGGTGTT ATGTGAAGAC 
CATCGGGGTC GCATGGTGAA GCACCAGTGC TGTCCTGGCT GCGGCTACTT CTGCACGGCG 
GGTAACTTCA TGGAATGCCA GCCTGAGAGC AGCATCTCTC ATCGTTTCCA TAAGGACTGT 
GCCTCTCGAG TCAACAATGC CAGCTACTGT CCCCATTGTG GGGAAGAAGC TTCCAAGGCC 
AAAGAGGTGA CCATAGCAAA AGCAGACACA ACTTCCACAG TGACCCTAGC CCCTGGACAG 
GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT GACACGACCA CGGGCAGCAT TGCTGGAGCC 
CCAGAGGATG AAAGATCGCA GAGCACAGCC CCCCAGGCAC CAGAGTGCTT CGACCCTGCC 
GGACCGGCTG GGCTCGTGAG GCCGACATCT GGCCTTTCCC AGGGCCCAGG AAAGGAAACC 
TTGGAAAGTG CTCTAATCGC TCTAGACTCT GAAAAACCCA AGAAACTTCG CTTCCACCCA 
AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT GAGCTGCAGA AGGTGCTTCT CATGCTGGTT 
GATGGAATTG ATCCCAACTT CAAAATGGAG CACCAAAGTA AGCGTTCCCC ATTACATGCT 
GCTGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGG TTCAGGCGGG TGCCAATATT 
GACACTTGCT CAGAGGACCA GCGGACCCCA CTGATGGAGG CTGCAGAGAA CAACCACTTG 
GATGCAGTGA AGTACCTCAT CAAGGCTGGA GCACAGGTGG ATCCGAAGGA CGCAGAGGGC 
TCCACATGTT TGCATTTGGC TGCCAAGAAA GGCCACTATG ATGTGGTTCA GTATCTGCTT 
TCAAATGGAC AGATGGATGT CAACTGCCAG GATGACGGTG GATGGACACC TATGATCTGG 
GCCACTGAGT ACAAGCACGT GGAGCTGGTG AAGCTGCTGC TGTCTAAGGG CTCTGACATC 
AACATCCGGG ACAACGAGGA GAACATTTGT CTGCACTGGG CAGCATTTTC AGGCTGTGTG 
GACATAGCTG AAATACTTCT GGCTGCCAAG TGTGACCTGC ATGCTGTGAA TATCCATGGA 
GACTCACCCC TGCACATCGC AGCCAGGGAG AATCGCTACG ACTGTGTTGT CCTCTTTCTT 
TCTCGGGATT CAGATGTTAC TCTGAAAAAC AAGGAAGGAG AGACTCCCTT GCAGTGTGCA 
AGTCTCAGTT CGCAGGTGTG GAGTGCATTG CAGATGAGCA AAGCACTTCG GGACTCAGCC 
CCTGACAAGC CCGTTGCTGT TGAGAAGACG GTGAGCAGGG ATATCGCTCG AGGGTATGAG 
CGCATTCCCA TTCCCTGTGT CAATGCTGTG GACAGTGAGC TGTGTCCTAC CAACTATAAG 
TATGTCTCCC AGAACTGTGT GACATCCCCC ATGAACATTG ACAGGAACAT CACTCATTTG 
CAGTACTGCG TGTGTGTAGA TGACTGCTCC TCTAGCACCT GCATGTGTGG CCAGCTGAGC 
ATGCGCTGCT GGTATGATAA GGATGGCCGA CTTCTGCCTG AGTTTAACAT GGCAGAACCA 
CCCTTGATCT TCGAGTGCAA TCATGCCTGC TCATGCTGGA GGAACTGCCG CAATCGTGTG 
GTGCAAAATG GTCTCAGGGC AAGGCTGCAG CTTTATCGGA CACAGGACAT GGGCTGGGGT 
GTGCGGTCCC TCCAGGATAT CCCACTGGGC ACCTTTGTCT GCGAATACGT AGGGGAGCTG 
ATTTCGGACT CTGAAGCTGA TGTTCGGGAA GAGGACTCTT ACCTCTTTGA TCTTGACAAT 
AAGGATGGAG AGGTATACTG CATTGACGCT CGGTTCTATG GGAATGTCAG CCGGTTCATA 
AACCACCACT GCGAACCCAA CCTTGTGCCT GTGCGAGTGT TCATGTCACA CCAGGACCTG 
CGGTTTCCCA GGATTGCCTT CTTCAGTACC CGCCTGATTC AGGCTGGGGA GCAGCTCGGG 
TTCGACTACG GGGAGCGCTT TTGGGACGTC AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG 
TCTTCCAAGT GTCGGCACTC AAGCGCAGCC CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG 
GAGCCTCAGG AGAATGGCCT TCCAGATACC AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108087.1
CDS	27..3746
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252227.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252227.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT 
AGTTTAGTTC CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT 
AAAAGCATAA CCCATTCCAC TGTGGGCGCC AAGATGGATG GAGATTCAGA GGAGGAACAG 
GAGTCTCCCG ACACAGGGGA GGATGAAGAT GGTGGAGACG AGTCTGACCT GAGTTCTGAA 
TCCAGTATCA AGAAGAAATT TCTCAAGAGG AGAGGGAAGA CTGACAGCCC CTGGATCAAA 
CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA AAGAAGCCGA GCAGCATGCT TGGTTCCGAG 
GCTTGTAAGT CATCTCCAGG AAGCATGGAG CAGGCAGCTC TGGGAGACAG CGCTGGCTAT 
ATGGAAGTTT CCCTGGATTC CCTGGATCTC CGTGTCAGAG GAATTCTGTC TTCCCAGACA 
GAAAATGAAG GGCTGGCCAG TGGTCCGGAT GTGCTGGGGA CGGATGGCCT CCAGGAAGTG 
CCTCTCTGCA GCTGCCGAAT GGAAACCCCC AAGAGCCGCG AGATCAGCAC CCTGGCCAAC 
AACCAGTGCA TGGCCACTGA GAGCGTGGAT CACGAATTGG GCCGGTGTAC AAACAGCGTG 
GTCAAGTATG AGCTGATGCG CCCATCCAAC AAAGCACCGC TCTTGGTGTT ATGTGAAGAC 
CATCGGGGTC GCATGGTGAA GCACCAGTGC TGTCCTGGCT GCGGCTACTT CTGCACGGCG 
GGTAACTTCA TGGAATGCCA GCCTGAGAGC AGCATCTCTC ATCGTTTCCA TAAGGACTGT 
GCCTCTCGAG TCAACAATGC CAGCTACTGT CCCCATTGTG GGGAAGAAGC TTCCAAGGCC 
AAAGAGGTGA CCATAGCAAA AGCAGACACA ACTTCCACAG TGACCCTAGC CCCTGGACAG 
GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT GACACGACCA CGGGCAGCAT TGCTGGAGCC 
CCAGAGGATG AAAGATCGCA GAGCACAGCC CCCCAGGCAC CAGAGTGCTT CGACCCTGCC 
GGACCGGCTG GGCTCGTGAG GCCGACATCT GGCCTTTCCC AGGGCCCAGG AAAGGAAACC 
TTGGAAAGTG CTCTAATCGC TCTAGACTCT GAAAAACCCA AGAAACTTCG CTTCCACCCA 
AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT GAGCTGCAGA AGGTGCTTCT CATGCTGGTT 
GATGGAATTG ATCCCAACTT CAAAATGGAG CACCAAAGTA AGCGTTCCCC ATTACATGCT 
GCTGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGG TTCAGGCGGG TGCCAATATT 
GACACTTGCT CAGAGGACCA GCGGACCCCA CTGATGGAGG CTGCAGAGAA CAACCACTTG 
GATGCAGTGA AGTACCTCAT CAAGGCTGGA GCACAGGTGG ATCCGAAGGA CGCAGAGGGC 
TCCACATGTT TGCATTTGGC TGCCAAGAAA GGCCACTATG ATGTGGTTCA GTATCTGCTT 
TCAAATGGAC AGATGGATGT CAACTGCCAG GATGACGGTG GATGGACACC TATGATCTGG 
GCCACTGAGT ACAAGCACGT GGAGCTGGTG AAGCTGCTGC TGTCTAAGGG CTCTGACATC 
AACATCCGGG ACAACGAGGA GAACATTTGT CTGCACTGGG CAGCATTTTC AGGCTGTGTG 
GACATAGCTG AAATACTTCT GGCTGCCAAG TGTGACCTGC ATGCTGTGAA TATCCATGGA 
GACTCACCCC TGCACATCGC AGCCAGGGAG AATCGCTACG ACTGTGTTGT CCTCTTTCTT 
TCTCGGGATT CAGATGTTAC TCTGAAAAAC AAGGAAGGAG AGACTCCCTT GCAGTGTGCA 
AGTCTCAGTT CGCAGGTGTG GAGTGCATTG CAGATGAGCA AAGCACTTCG GGACTCAGCC 
CCTGACAAGC CCGTTGCTGT TGAGAAGACG GTGAGCAGGG ATATCGCTCG AGGGTATGAG 
CGCATTCCCA TTCCCTGTGT CAATGCTGTG GACAGTGAGC TGTGTCCTAC CAACTATAAG 
TATGTCTCCC AGAACTGTGT GACATCCCCC ATGAACATTG ACAGGAACAT CACTCATTTG 
CAGTACTGCG TGTGTGTAGA TGACTGCTCC TCTAGCACCT GCATGTGTGG CCAGCTGAGC 
ATGCGCTGCT GGTATGATAA GGATGGCCGA CTTCTGCCTG AGTTTAACAT GGCAGAACCA 
CCCTTGATCT TCGAGTGCAA TCATGCCTGC TCATGCTGGA GGAACTGCCG CAATCGTGTG 
GTGCAAAATG GTCTCAGGGC AAGGCTGCAG CTTTATCGGA CACAGGACAT GGGCTGGGGT 
GTGCGGTCCC TCCAGGATAT CCCACTGGGC ACCTTTGTCT GCGAATACGT AGGGGAGCTG 
ATTTCGGACT CTGAAGCTGA TGTTCGGGAA GAGGACTCTT ACCTCTTTGA TCTTGACAAT 
AAGGATGGAG AGGTATACTG CATTGACGCT CGGTTCTATG GGAATGTCAG CCGGTTCATA 
AACCACCACT GCGAACCCAA CCTTGTGCCT GTGCGAGTGT TCATGTCACA CCAGGACCTG 
CGGTTTCCCA GGATTGCCTT CTTCAGTACC CGCCTGATTC AGGCTGGGGA GCAGCTCGGG 
TTCGACTACG GGGAGCGCTT TTGGGACGTC AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG 
TCTTCCAAGT GTCGGCACTC AAGCGCAGCC CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG 
GAGCCTCAGG AGAATGGCCT TCCAGATACC AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252228.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108088.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu88064
Clone ID Related Accession (Same CDS sequence)	XM_030252228.1
Accession Version	XM_030252228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAAGGT 
AACTTCATGG AATGCCAGCC TGAGAGCAGC ATCTCTCATC GTTTCCATAA GGACTGTGCC 
TCTCGAGTCA ACAATGCCAG CTACTGTCCC CATTGTGGGG AAGAAGCTTC CAAGGCCAAA 
GAGGTGACCA TAGCAAAAGC AGACACAACT TCCACAGTGA CCCTAGCCCC TGGACAGGAG 
AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC ACGACCACGG GCAGCATTGC TGGAGCCCCA 
GAGGATGAAA GATCGCAGAG CACAGCCCCC CAGGCACCAG AGTGCTTCGA CCCTGCCGGA 
CCGGCTGGGC TCGTGAGGCC GACATCTGGC CTTTCCCAGG GCCCAGGAAA GGAAACCTTG 
GAAAGTGCTC TAATCGCTCT AGACTCTGAA AAACCCAAGA AACTTCGCTT CCACCCAAAG 
CAGCTGTACT TCTCTGCCAG GCAGGGTGAG CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT 
GGAATTGATC CCAACTTCAA AATGGAGCAC CAAAGTAAGC GTTCCCCATT ACATGCTGCT 
GCGGAGGCTG GCCACGTGGA CATCTGCCAC ATGCTGGTTC AGGCGGGTGC CAATATTGAC 
ACTTGCTCAG AGGACCAGCG GACCCCACTG ATGGAGGCTG CAGAGAACAA CCACTTGGAT 
GCAGTGAAGT ACCTCATCAA GGCTGGAGCA CAGGTGGATC CGAAGGACGC AGAGGGCTCC 
ACATGTTTGC ATTTGGCTGC CAAGAAAGGC CACTATGATG TGGTTCAGTA TCTGCTTTCA 
AATGGACAGA TGGATGTCAA CTGCCAGGAT GACGGTGGAT GGACACCTAT GATCTGGGCC 
ACTGAGTACA AGCACGTGGA GCTGGTGAAG CTGCTGCTGT CTAAGGGCTC TGACATCAAC 
ATCCGGGACA ACGAGGAGAA CATTTGTCTG CACTGGGCAG CATTTTCAGG CTGTGTGGAC 
ATAGCTGAAA TACTTCTGGC TGCCAAGTGT GACCTGCATG CTGTGAATAT CCATGGAGAC 
TCACCCCTGC ACATCGCAGC CAGGGAGAAT CGCTACGACT GTGTTGTCCT CTTTCTTTCT 
CGGGATTCAG ATGTTACTCT GAAAAACAAG GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT 
CTCAGTTCGC AGGTGTGGAG TGCATTGCAG ATGAGCAAAG CACTTCGGGA CTCAGCCCCT 
GACAAGCCCG TTGCTGTTGA GAAGACGGTG AGCAGGGATA TCGCTCGAGG GTATGAGCGC 
ATTCCCATTC CCTGTGTCAA TGCTGTGGAC AGTGAGCTGT GTCCTACCAA CTATAAGTAT 
GTCTCCCAGA ACTGTGTGAC ATCCCCCATG AACATTGACA GGAACATCAC TCATTTGCAG 
TACTGCGTGT GTGTAGATGA CTGCTCCTCT AGCACCTGCA TGTGTGGCCA GCTGAGCATG 
CGCTGCTGGT ATGATAAGGA TGGCCGACTT CTGCCTGAGT TTAACATGGC AGAACCACCC 
TTGATCTTCG AGTGCAATCA TGCCTGCTCA TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG 
CAAAATGGTC TCAGGGCAAG GCTGCAGCTT TATCGGACAC AGGACATGGG CTGGGGTGTG 
CGGTCCCTCC AGGATATCCC ACTGGGCACC TTTGTCTGCG AATACGTAGG GGAGCTGATT 
TCGGACTCTG AAGCTGATGT TCGGGAAGAG GACTCTTACC TCTTTGATCT TGACAATAAG 
GATGGAGAGG TATACTGCAT TGACGCTCGG TTCTATGGGA ATGTCAGCCG GTTCATAAAC 
CACCACTGCG AACCCAACCT TGTGCCTGTG CGAGTGTTCA TGTCACACCA GGACCTGCGG 
TTTCCCAGGA TTGCCTTCTT CAGTACCCGC CTGATTCAGG CTGGGGAGCA GCTCGGGTTC 
GACTACGGGG AGCGCTTTTG GGACGTCAAG GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT 
TCCAAGTGTC GGCACTCAAG CGCAGCCCTG GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG 
CCTCAGGAGA ATGGCCTTCC AGATACCAGC TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108088.1
CDS	27..3683
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252228.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252228.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAAGGT 
AACTTCATGG AATGCCAGCC TGAGAGCAGC ATCTCTCATC GTTTCCATAA GGACTGTGCC 
TCTCGAGTCA ACAATGCCAG CTACTGTCCC CATTGTGGGG AAGAAGCTTC CAAGGCCAAA 
GAGGTGACCA TAGCAAAAGC AGACACAACT TCCACAGTGA CCCTAGCCCC TGGACAGGAG 
AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC ACGACCACGG GCAGCATTGC TGGAGCCCCA 
GAGGATGAAA GATCGCAGAG CACAGCCCCC CAGGCACCAG AGTGCTTCGA CCCTGCCGGA 
CCGGCTGGGC TCGTGAGGCC GACATCTGGC CTTTCCCAGG GCCCAGGAAA GGAAACCTTG 
GAAAGTGCTC TAATCGCTCT AGACTCTGAA AAACCCAAGA AACTTCGCTT CCACCCAAAG 
CAGCTGTACT TCTCTGCCAG GCAGGGTGAG CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT 
GGAATTGATC CCAACTTCAA AATGGAGCAC CAAAGTAAGC GTTCCCCATT ACATGCTGCT 
GCGGAGGCTG GCCACGTGGA CATCTGCCAC ATGCTGGTTC AGGCGGGTGC CAATATTGAC 
ACTTGCTCAG AGGACCAGCG GACCCCACTG ATGGAGGCTG CAGAGAACAA CCACTTGGAT 
GCAGTGAAGT ACCTCATCAA GGCTGGAGCA CAGGTGGATC CGAAGGACGC AGAGGGCTCC 
ACATGTTTGC ATTTGGCTGC CAAGAAAGGC CACTATGATG TGGTTCAGTA TCTGCTTTCA 
AATGGACAGA TGGATGTCAA CTGCCAGGAT GACGGTGGAT GGACACCTAT GATCTGGGCC 
ACTGAGTACA AGCACGTGGA GCTGGTGAAG CTGCTGCTGT CTAAGGGCTC TGACATCAAC 
ATCCGGGACA ACGAGGAGAA CATTTGTCTG CACTGGGCAG CATTTTCAGG CTGTGTGGAC 
ATAGCTGAAA TACTTCTGGC TGCCAAGTGT GACCTGCATG CTGTGAATAT CCATGGAGAC 
TCACCCCTGC ACATCGCAGC CAGGGAGAAT CGCTACGACT GTGTTGTCCT CTTTCTTTCT 
CGGGATTCAG ATGTTACTCT GAAAAACAAG GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT 
CTCAGTTCGC AGGTGTGGAG TGCATTGCAG ATGAGCAAAG CACTTCGGGA CTCAGCCCCT 
GACAAGCCCG TTGCTGTTGA GAAGACGGTG AGCAGGGATA TCGCTCGAGG GTATGAGCGC 
ATTCCCATTC CCTGTGTCAA TGCTGTGGAC AGTGAGCTGT GTCCTACCAA CTATAAGTAT 
GTCTCCCAGA ACTGTGTGAC ATCCCCCATG AACATTGACA GGAACATCAC TCATTTGCAG 
TACTGCGTGT GTGTAGATGA CTGCTCCTCT AGCACCTGCA TGTGTGGCCA GCTGAGCATG 
CGCTGCTGGT ATGATAAGGA TGGCCGACTT CTGCCTGAGT TTAACATGGC AGAACCACCC 
TTGATCTTCG AGTGCAATCA TGCCTGCTCA TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG 
CAAAATGGTC TCAGGGCAAG GCTGCAGCTT TATCGGACAC AGGACATGGG CTGGGGTGTG 
CGGTCCCTCC AGGATATCCC ACTGGGCACC TTTGTCTGCG AATACGTAGG GGAGCTGATT 
TCGGACTCTG AAGCTGATGT TCGGGAAGAG GACTCTTACC TCTTTGATCT TGACAATAAG 
GATGGAGAGG TATACTGCAT TGACGCTCGG TTCTATGGGA ATGTCAGCCG GTTCATAAAC 
CACCACTGCG AACCCAACCT TGTGCCTGTG CGAGTGTTCA TGTCACACCA GGACCTGCGG 
TTTCCCAGGA TTGCCTTCTT CAGTACCCGC CTGATTCAGG CTGGGGAGCA GCTCGGGTTC 
GACTACGGGG AGCGCTTTTG GGACGTCAAG GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT 
TCCAAGTGTC GGCACTCAAG CGCAGCCCTG GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG 
CCTCAGGAGA ATGGCCTTCC AGATACCAGC TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252229.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108089.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X33, mRNA.

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CloneID	OMu88065
Clone ID Related Accession (Same CDS sequence)	XM_030252229.1
Accession Version	XM_030252229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGGGC 
GAGAAAGCCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCA 
GAAGATGAAG ACTCCGATGA GCTGGAGGAT GACGAGGACC ATGGAGCTGA GCAAGCGGCT 
GCATTTCCCA CCGAGGATAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGACCGGGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CCGACACAGG GGAGGATGAA 
GATGGTGGAG ACGAGTCTGA CCTGAGTTCT GAATCCAGTA TCAAGAAGAA ATTTCTCAAG 
AGGAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGT 
AGAAAGAAGC CGAGCAGCAT GCTTGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108089.1
CDS	27..3677
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252229.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252229.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG ATACACCTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAACA AGAAGGAGAG 
ACTCCCATGG CTGCAGATGG AGAAACAAAT GGGTCTTGTG AAAAGAGTGG GGATCCCAGC 
CATCTAAATG CACCCAAACA CACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTGT CTCGGGTGGC AGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TCACAGCTGA CGACTTCATG CAGACATCTG TCATTGGCAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGGCAG CCGTTGAGGA CTCCCAACAT TCTAACCTCC 
TCGCTTCCTG GTCATGCTGC AAAAACTCTT CCTGGAGGAG CCAGTAAATG CAGGACTCTG 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCTT GGGCCTGCAT GCAGCCAGCA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CCTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAGTG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT ATCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGTTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAA 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATAACCCATT CCACTGTGGG CGCCAAGGGC 
GAGAAAGCCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCA 
GAAGATGAAG ACTCCGATGA GCTGGAGGAT GACGAGGACC ATGGAGCTGA GCAAGCGGCT 
GCATTTCCCA CCGAGGATAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGACCGGGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CCGACACAGG GGAGGATGAA 
GATGGTGGAG ACGAGTCTGA CCTGAGTTCT GAATCCAGTA TCAAGAAGAA ATTTCTCAAG 
AGGAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGT 
AGAAAGAAGC CGAGCAGCAT GCTTGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA AGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252230.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108090.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OMu88066
Clone ID Related Accession (Same CDS sequence)	XM_030252230.1
Accession Version	XM_030252230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGCCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAAGCTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACAAC TTCCACAGTG ACCCTAGCCC CTGGACAGGA GAAGAGCCTG 
GCTGCTGAAG GCAGGGCTGA CACGACCACG GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AGATCGCAGA GCACAGCCCC CCAGGCACCA GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG 
CTCGTGAGGC CGACATCTGG CCTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTAATCGCTC TAGACTCTGA AAAACCCAAG AAACTTCGCT TCCACCCAAA GCAGCTGTAC 
TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACATGCTGC TGCGGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGC GGACCCCACT GATGGAGGCT GCAGAGAACA ACCACTTGGA TGCAGTGAAG 
TACCTCATCA AGGCTGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACATGTTTG 
CATTTGGCTG CCAAGAAAGG CCACTATGAT GTGGTTCAGT ATCTGCTTTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGACGGTGGA TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG AGCTGGTGAA GCTGCTGCTG TCTAAGGGCT CTGACATCAA CATCCGGGAC 
AACGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATACTTCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCATGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTC TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG 
CAGGTGTGGA GTGCATTGCA GATGAGCAAA GCACTTCGGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGGGAT ATCGCTCGAG GGTATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG TGTCCTACCA ACTATAAGTA TGTCTCCCAG 
AACTGTGTGA CATCCCCCAT GAACATTGAC AGGAACATCA CTCATTTGCA GTACTGCGTG 
TGTGTAGATG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGATAAGG ATGGCCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC ATGCTGGAGG AACTGCCGCA ATCGTGTGGT GCAAAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTC 
CAGGATATCC CACTGGGCAC CTTTGTCTGC GAATACGTAG GGGAGCTGAT TTCGGACTCT 
GAAGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTATACTGCA TTGACGCTCG GTTCTATGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAACCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTGCG GTTTCCCAGG 
ATTGCCTTCT TCAGTACCCG CCTGATTCAG GCTGGGGAGC AGCTCGGGTT CGACTACGGG 
GAGCGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT 
CGGCACTCAA GCGCAGCCCT GGCCCAGAGG CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCC GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108090.1
CDS	18..3665
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252230.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252230.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGCCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAAGCTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACAAC TTCCACAGTG ACCCTAGCCC CTGGACAGGA GAAGAGCCTG 
GCTGCTGAAG GCAGGGCTGA CACGACCACG GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AGATCGCAGA GCACAGCCCC CCAGGCACCA GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG 
CTCGTGAGGC CGACATCTGG CCTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTAATCGCTC TAGACTCTGA AAAACCCAAG AAACTTCGCT TCCACCCAAA GCAGCTGTAC 
TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACATGCTGC TGCGGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGC GGACCCCACT GATGGAGGCT GCAGAGAACA ACCACTTGGA TGCAGTGAAG 
TACCTCATCA AGGCTGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACATGTTTG 
CATTTGGCTG CCAAGAAAGG CCACTATGAT GTGGTTCAGT ATCTGCTTTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGACGGTGGA TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG AGCTGGTGAA GCTGCTGCTG TCTAAGGGCT CTGACATCAA CATCCGGGAC 
AACGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATACTTCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCATGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTC TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG 
CAGGTGTGGA GTGCATTGCA GATGAGCAAA GCACTTCGGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGGGAT ATCGCTCGAG GGTATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG TGTCCTACCA ACTATAAGTA TGTCTCCCAG 
AACTGTGTGA CATCCCCCAT GAACATTGAC AGGAACATCA CTCATTTGCA GTACTGCGTG 
TGTGTAGATG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGATAAGG ATGGCCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC ATGCTGGAGG AACTGCCGCA ATCGTGTGGT GCAAAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTC 
CAGGATATCC CACTGGGCAC CTTTGTCTGC GAATACGTAG GGGAGCTGAT TTCGGACTCT 
GAAGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTATACTGCA TTGACGCTCG GTTCTATGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAACCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTGCG GTTTCCCAGG 
ATTGCCTTCT TCAGTACCCG CCTGATTCAG GCTGGGGAGC AGCTCGGGTT CGACTACGGG 
GAGCGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT 
CGGCACTCAA GCGCAGCCCT GGCCCAGAGG CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCC GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_030252231.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108091.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OMu88067
Clone ID Related Accession (Same CDS sequence)	XM_030252231.1
Accession Version	XM_030252231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAATTGGGC 
CGGTGTACAA ACAGCGTGGT CAAGTATGAG CTGATGCGCC CATCCAACAA AGCACCGCTC 
TTGGTGTTAT GTGAAGACCA TCGGGGTCGC ATGGTGAAGC ACCAGTGCTG TCCTGGCTGC 
GGCTACTTCT GCACGGCGGG TAACTTCATG GAATGCCAGC CTGAGAGCAG CATCTCTCAT 
CGTTTCCATA AGGACTGTGC CTCTCGAGTC AACAATGCCA GCTACTGTCC CCATTGTGGG 
GAAGAAGCTT CCAAGGCCAA AGAGGTGACC ATAGCAAAAG CAGACACAAC TTCCACAGTG 
ACCCTAGCCC CTGGACAGGA GAAGAGCCTG GCTGCTGAAG GCAGGGCTGA CACGACCACG 
GGCAGCATTG CTGGAGCCCC AGAGGATGAA AGATCGCAGA GCACAGCCCC CCAGGCACCA 
GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG CTCGTGAGGC CGACATCTGG CCTTTCCCAG 
GGCCCAGGAA AGGAAACCTT GGAAAGTGCT CTAATCGCTC TAGACTCTGA AAAACCCAAG 
AAACTTCGCT TCCACCCAAA GCAGCTGTAC TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG 
GTGCTTCTCA TGCTGGTTGA TGGAATTGAT CCCAACTTCA AAATGGAGCA CCAAAGTAAG 
CGTTCCCCAT TACATGCTGC TGCGGAGGCT GGCCACGTGG ACATCTGCCA CATGCTGGTT 
CAGGCGGGTG CCAATATTGA CACTTGCTCA GAGGACCAGC GGACCCCACT GATGGAGGCT 
GCAGAGAACA ACCACTTGGA TGCAGTGAAG TACCTCATCA AGGCTGGAGC ACAGGTGGAT 
CCGAAGGACG CAGAGGGCTC CACATGTTTG CATTTGGCTG CCAAGAAAGG CCACTATGAT 
GTGGTTCAGT ATCTGCTTTC AAATGGACAG ATGGATGTCA ACTGCCAGGA TGACGGTGGA 
TGGACACCTA TGATCTGGGC CACTGAGTAC AAGCACGTGG AGCTGGTGAA GCTGCTGCTG 
TCTAAGGGCT CTGACATCAA CATCCGGGAC AACGAGGAGA ACATTTGTCT GCACTGGGCA 
GCATTTTCAG GCTGTGTGGA CATAGCTGAA ATACTTCTGG CTGCCAAGTG TGACCTGCAT 
GCTGTGAATA TCCATGGAGA CTCACCCCTG CACATCGCAG CCAGGGAGAA TCGCTACGAC 
TGTGTTGTCC TCTTTCTTTC TCGGGATTCA GATGTTACTC TGAAAAACAA GGAAGGAGAG 
ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG CAGGTGTGGA GTGCATTGCA GATGAGCAAA 
GCACTTCGGG ACTCAGCCCC TGACAAGCCC GTTGCTGTTG AGAAGACGGT GAGCAGGGAT 
ATCGCTCGAG GGTATGAGCG CATTCCCATT CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG 
TGTCCTACCA ACTATAAGTA TGTCTCCCAG AACTGTGTGA CATCCCCCAT GAACATTGAC 
AGGAACATCA CTCATTTGCA GTACTGCGTG TGTGTAGATG ACTGCTCCTC TAGCACCTGC 
ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG TATGATAAGG ATGGCCGACT TCTGCCTGAG 
TTTAACATGG CAGAACCACC CTTGATCTTC GAGTGCAATC ATGCCTGCTC ATGCTGGAGG 
AACTGCCGCA ATCGTGTGGT GCAAAATGGT CTCAGGGCAA GGCTGCAGCT TTATCGGACA 
CAGGACATGG GCTGGGGTGT GCGGTCCCTC CAGGATATCC CACTGGGCAC CTTTGTCTGC 
GAATACGTAG GGGAGCTGAT TTCGGACTCT GAAGCTGATG TTCGGGAAGA GGACTCTTAC 
CTCTTTGATC TTGACAATAA GGATGGAGAG GTATACTGCA TTGACGCTCG GTTCTATGGG 
AATGTCAGCC GGTTCATAAA CCACCACTGC GAACCCAACC TTGTGCCTGT GCGAGTGTTC 
ATGTCACACC AGGACCTGCG GTTTCCCAGG ATTGCCTTCT TCAGTACCCG CCTGATTCAG 
GCTGGGGAGC AGCTCGGGTT CGACTACGGG GAGCGCTTTT GGGACGTCAA GGGCAAGCTC 
TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT CGGCACTCAA GCGCAGCCCT GGCCCAGAGG 
CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG AATGGCCTTC CAGATACCAG CTCTGCAGCC 
GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108091.1
CDS	379..3996
Translation	MKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_030252231.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252231.1

ORF Insert Sequence:

ATGAAAACTG AGCTGCTAAG GGAAGATACA CCTATGGCTG CTGATGAAGG TTCCACAGAG 
AAACAAGAAG GAGAGACTCC CATGGCTGCA GATGGAGAAA CAAATGGGTC TTGTGAAAAG 
AGTGGGGATC CCAGCCATCT AAATGCACCC AAACACACTC AGGAGAACAC AAGAGCTAGC 
CCACAGGAAG GCACCAACAG AGTGTCTCGG GTGGCAGAAA ATGGGGTTTC AGAAAGAGAC 
ACAGAAGTGG GGAAGCAAAA CCATGTCACA GCTGACGACT TCATGCAGAC ATCTGTCATT 
GGCAGCAATG GATATTTCTT AAATAAACCA GCCCTGCAGG GGCAGCCGTT GAGGACTCCC 
AACATTCTAA CCTCCTCGCT TCCTGGTCAT GCTGCAAAAA CTCTTCCTGG AGGAGCCAGT 
AAATGCAGGA CTCTGAGTGC ACTTCCTCAG ACACCAACCA CAGCACCCAC TGTGCCTGGG 
GAAGGGAGTG CAGACACAGA GGACAGAAAG CCTACAGCCT CGGGCACTGA TGTCAGGGTT 
CACAGGGCAC GCAAGACCAT GCCGAAGTCC ATCTTGGGCC TGCATGCAGC CAGCAAAGAC 
CATAGAGAAG TTCAAGACCA TAAGGAACCA AAAGAGGATA TCAACAGAAA CATTTCTGAA 
TGTGGACGAC AGCAGCTTTT ACCAACCTTC CCAGCCCTCC ACCAGTCGCT ACCTCAGAAT 
CAGTGCTACA TGGCCACCAC AAAGTCCCAG ACAGCAGCTG CAGTATCTCG GAAGAAAAAA 
CGAAGAATGG GAACCTATAG TTTAGTTCCC AAGAAAAAGA CCAAAGTATT AAAACAGAGG 
ACGGTGATTG AGATGTTTAA AAGCATAACC CATTCCACTG TGGGCGCCAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAATTGGGC 
CGGTGTACAA ACAGCGTGGT CAAGTATGAG CTGATGCGCC CATCCAACAA AGCACCGCTC 
TTGGTGTTAT GTGAAGACCA TCGGGGTCGC ATGGTGAAGC ACCAGTGCTG TCCTGGCTGC 
GGCTACTTCT GCACGGCGGG TAACTTCATG GAATGCCAGC CTGAGAGCAG CATCTCTCAT 
CGTTTCCATA AGGACTGTGC CTCTCGAGTC AACAATGCCA GCTACTGTCC CCATTGTGGG 
GAAGAAGCTT CCAAGGCCAA AGAGGTGACC ATAGCAAAAG CAGACACAAC TTCCACAGTG 
ACCCTAGCCC CTGGACAGGA GAAGAGCCTG GCTGCTGAAG GCAGGGCTGA CACGACCACG 
GGCAGCATTG CTGGAGCCCC AGAGGATGAA AGATCGCAGA GCACAGCCCC CCAGGCACCA 
GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG CTCGTGAGGC CGACATCTGG CCTTTCCCAG 
GGCCCAGGAA AGGAAACCTT GGAAAGTGCT CTAATCGCTC TAGACTCTGA AAAACCCAAG 
AAACTTCGCT TCCACCCAAA GCAGCTGTAC TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG 
GTGCTTCTCA TGCTGGTTGA TGGAATTGAT CCCAACTTCA AAATGGAGCA CCAAAGTAAG 
CGTTCCCCAT TACATGCTGC TGCGGAGGCT GGCCACGTGG ACATCTGCCA CATGCTGGTT 
CAGGCGGGTG CCAATATTGA CACTTGCTCA GAGGACCAGC GGACCCCACT GATGGAGGCT 
GCAGAGAACA ACCACTTGGA TGCAGTGAAG TACCTCATCA AGGCTGGAGC ACAGGTGGAT 
CCGAAGGACG CAGAGGGCTC CACATGTTTG CATTTGGCTG CCAAGAAAGG CCACTATGAT 
GTGGTTCAGT ATCTGCTTTC AAATGGACAG ATGGATGTCA ACTGCCAGGA TGACGGTGGA 
TGGACACCTA TGATCTGGGC CACTGAGTAC AAGCACGTGG AGCTGGTGAA GCTGCTGCTG 
TCTAAGGGCT CTGACATCAA CATCCGGGAC AACGAGGAGA ACATTTGTCT GCACTGGGCA 
GCATTTTCAG GCTGTGTGGA CATAGCTGAA ATACTTCTGG CTGCCAAGTG TGACCTGCAT 
GCTGTGAATA TCCATGGAGA CTCACCCCTG CACATCGCAG CCAGGGAGAA TCGCTACGAC 
TGTGTTGTCC TCTTTCTTTC TCGGGATTCA GATGTTACTC TGAAAAACAA GGAAGGAGAG 
ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG CAGGTGTGGA GTGCATTGCA GATGAGCAAA 
GCACTTCGGG ACTCAGCCCC TGACAAGCCC GTTGCTGTTG AGAAGACGGT GAGCAGGGAT 
ATCGCTCGAG GGTATGAGCG CATTCCCATT CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG 
TGTCCTACCA ACTATAAGTA TGTCTCCCAG AACTGTGTGA CATCCCCCAT GAACATTGAC 
AGGAACATCA CTCATTTGCA GTACTGCGTG TGTGTAGATG ACTGCTCCTC TAGCACCTGC 
ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG TATGATAAGG ATGGCCGACT TCTGCCTGAG 
TTTAACATGG CAGAACCACC CTTGATCTTC GAGTGCAATC ATGCCTGCTC ATGCTGGAGG 
AACTGCCGCA ATCGTGTGGT GCAAAATGGT CTCAGGGCAA GGCTGCAGCT TTATCGGACA 
CAGGACATGG GCTGGGGTGT GCGGTCCCTC CAGGATATCC CACTGGGCAC CTTTGTCTGC 
GAATACGTAG GGGAGCTGAT TTCGGACTCT GAAGCTGATG TTCGGGAAGA GGACTCTTAC 
CTCTTTGATC TTGACAATAA GGATGGAGAG GTATACTGCA TTGACGCTCG GTTCTATGGG 
AATGTCAGCC GGTTCATAAA CCACCACTGC GAACCCAACC TTGTGCCTGT GCGAGTGTTC 
ATGTCACACC AGGACCTGCG GTTTCCCAGG ATTGCCTTCT TCAGTACCCG CCTGATTCAG 
GCTGGGGAGC AGCTCGGGTT CGACTACGGG GAGCGCTTTT GGGACGTCAA GGGCAAGCTC 
TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT CGGCACTCAA GCGCAGCCCT GGCCCAGAGG 
CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG AATGGCCTTC CAGATACCAG CTCTGCAGCC 
GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( NM_172545.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766133.2 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 2, mRNA.

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CloneID	OMu16352
Clone ID Related Accession (Same CDS sequence)	NM_172545.4
Accession Version	NM_172545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BB621957.1, AK086833.1, AK049454.1 and DV649682.1. On Mar 9, 2013 this sequence version replaced NM_172545.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766133.2
CDS	36..3905
Translation	MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	NM_172545.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172545.4

ORF Insert Sequence:

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( NM_001109686.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103156.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu21886
Clone ID Related Accession (Same CDS sequence)	NM_001109686.2
Accession Version	NM_001109686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BB621957.1, BC089302.1 and DV649682.1. On Mar 9, 2013 this sequence version replaced NM_001109686.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103156.1
CDS	36..3767
Translation	MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	NM_001109686.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109686.2

ORF Insert Sequence:

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGGCTGGCC AGTGGTCCGG ATGTGCTGGG GACGGATGGC 
CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA ATGGAAACCC CCAAGAGCCG CGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCATCCA ACAAAGCACC GCTCTTGGTG 
TTATGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGC CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAA 
GCTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CAACTTCCAC AGTGACCCTA 
GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT GAAGGCAGGG CTGACACGAC CACGGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAGATCG CAGAGCACAG CCCCCCAGGC ACCAGAGTGC 
TTCGACCCTG CCGGACCGGC TGGGCTCGTG AGGCCGACAT CTGGCCTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTAATC GCTCTAGACT CTGAAAAACC CAAGAAACTT 
CGCTTCCACC CAAAGCAGCT GTACTTCTCT GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACATG CTGCTGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGCGGACCC CACTGATGGA GGCTGCAGAG 
AACAACCACT TGGATGCAGT GAAGTACCTC ATCAAGGCTG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACATG TTTGCATTTG GCTGCCAAGA AAGGCCACTA TGATGTGGTT 
CAGTATCTGC TTTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGACGG TGGATGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG 
GGCTCTGACA TCAACATCCG GGACAACGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATACTT CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCATG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTCTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG TGGAGTGCAT TGCAGATGAG CAAAGCACTT 
CGGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG GGATATCGCT 
CGAGGGTATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGACAGTGA GCTGTGTCCT 
ACCAACTATA AGTATGTCTC CCAGAACTGT GTGACATCCC CCATGAACAT TGACAGGAAC 
ATCACTCATT TGCAGTACTG CGTGTGTGTA GATGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAT AAGGATGGCC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCATGCTG GAGGAACTGC 
CGCAATCGTG TGGTGCAAAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT ATCCCACTGG GCACCTTTGT CTGCGAATAC 
GTAGGGGAGC TGATTTCGGA CTCTGAAGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTATAC TGCATTGACG CTCGGTTCTA TGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAACCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TGCGGTTTCC CAGGATTGCC TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG 
GAGCAGCTCG GGTTCGACTA CGGGGAGCGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC 
AGTGCAGCCC AGGAGCCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCCGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( NM_001109687.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103157.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu21894
Clone ID Related Accession (Same CDS sequence)	NM_001109687.2
Accession Version	NM_001109687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BB621957.1, BC056938.1 and DV649682.1. On Mar 9, 2013 this sequence version replaced NM_001109687.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTAT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGTTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAACA GAGGACGGTG ATTGAGATGT TTAAAAGCAT AACCCATTCC 
ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCAGA AGATGAAGAC TCCGATGAGC TGGAGGATGA CGAGGACCAT 
GGAGCTGAGC AAGCGGCTGC ATTTCCCACC GAGGATAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ACCGGGCTGC AAAGATGGAT GGAGATTCAG AGGAGGAACA GGAGTCTCCC 
GACACAGGGG AGGATGAAGA TGGTGGAGAC GAGTCTGACC TGAGTTCTGA ATCCAGTATC 
AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG ACTGACAGCC CCTGGATCAA ACCTGCTCGG 
AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG AGCAGCATGC TTGGTTCCGA GGCTTGTAAG 
TCATCTCCAG GAAGCATGGA GCAGGCAGCT CTGGGAGACA GCGCTGGCTA TATGGAAGTT 
TCCCTGGATT CCCTGGATCT CCGTGTCAGA GGAATTCTGT CTTCCCAGAC AGAAAATGAA 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAAGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103157.1
CDS	36..3782
Translation	MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	NM_001109687.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109687.2

ORF Insert Sequence:

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTAT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGTTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAACA GAGGACGGTG ATTGAGATGT TTAAAAGCAT AACCCATTCC 
ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCAGA AGATGAAGAC TCCGATGAGC TGGAGGATGA CGAGGACCAT 
GGAGCTGAGC AAGCGGCTGC ATTTCCCACC GAGGATAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ACCGGGCTGC AAAGATGGAT GGAGATTCAG AGGAGGAACA GGAGTCTCCC 
GACACAGGGG AGGATGAAGA TGGTGGAGAC GAGTCTGACC TGAGTTCTGA ATCCAGTATC 
AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG ACTGACAGCC CCTGGATCAA ACCTGCTCGG 
AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG AGCAGCATGC TTGGTTCCGA GGCTTGTAAG 
TCATCTCCAG GAAGCATGGA GCAGGCAGCT CTGGGAGACA GCGCTGGCTA TATGGAAGTT 
TCCCTGGATT CCCTGGATCT CCGTGTCAGA GGAATTCTGT CTTCCCAGAC AGAAAATGAA 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAAGGT AACTTCATGG AATGCCAGCC TGAGAGCAGC 
ATCTCTCATC GTTTCCATAA GGACTGTGCC TCTCGAGTCA ACAATGCCAG CTACTGTCCC 
CATTGTGGGG AAGAAGCTTC CAAGGCCAAA GAGGTGACCA TAGCAAAAGC AGACACAACT 
TCCACAGTGA CCCTAGCCCC TGGACAGGAG AAGAGCCTGG CTGCTGAAGG CAGGGCTGAC 
ACGACCACGG GCAGCATTGC TGGAGCCCCA GAGGATGAAA GATCGCAGAG CACAGCCCCC 
CAGGCACCAG AGTGCTTCGA CCCTGCCGGA CCGGCTGGGC TCGTGAGGCC GACATCTGGC 
CTTTCCCAGG GCCCAGGAAA GGAAACCTTG GAAAGTGCTC TAATCGCTCT AGACTCTGAA 
AAACCCAAGA AACTTCGCTT CCACCCAAAG CAGCTGTACT TCTCTGCCAG GCAGGGTGAG 
CTGCAGAAGG TGCTTCTCAT GCTGGTTGAT GGAATTGATC CCAACTTCAA AATGGAGCAC 
CAAAGTAAGC GTTCCCCATT ACATGCTGCT GCGGAGGCTG GCCACGTGGA CATCTGCCAC 
ATGCTGGTTC AGGCGGGTGC CAATATTGAC ACTTGCTCAG AGGACCAGCG GACCCCACTG 
ATGGAGGCTG CAGAGAACAA CCACTTGGAT GCAGTGAAGT ACCTCATCAA GGCTGGAGCA 
CAGGTGGATC CGAAGGACGC AGAGGGCTCC ACATGTTTGC ATTTGGCTGC CAAGAAAGGC 
CACTATGATG TGGTTCAGTA TCTGCTTTCA AATGGACAGA TGGATGTCAA CTGCCAGGAT 
GACGGTGGAT GGACACCTAT GATCTGGGCC ACTGAGTACA AGCACGTGGA GCTGGTGAAG 
CTGCTGCTGT CTAAGGGCTC TGACATCAAC ATCCGGGACA ACGAGGAGAA CATTTGTCTG 
CACTGGGCAG CATTTTCAGG CTGTGTGGAC ATAGCTGAAA TACTTCTGGC TGCCAAGTGT 
GACCTGCATG CTGTGAATAT CCATGGAGAC TCACCCCTGC ACATCGCAGC CAGGGAGAAT 
CGCTACGACT GTGTTGTCCT CTTTCTTTCT CGGGATTCAG ATGTTACTCT GAAAAACAAG 
GAAGGAGAGA CTCCCTTGCA GTGTGCAAGT CTCAGTTCGC AGGTGTGGAG TGCATTGCAG 
ATGAGCAAAG CACTTCGGGA CTCAGCCCCT GACAAGCCCG TTGCTGTTGA GAAGACGGTG 
AGCAGGGATA TCGCTCGAGG GTATGAGCGC ATTCCCATTC CCTGTGTCAA TGCTGTGGAC 
AGTGAGCTGT GTCCTACCAA CTATAAGTAT GTCTCCCAGA ACTGTGTGAC ATCCCCCATG 
AACATTGACA GGAACATCAC TCATTTGCAG TACTGCGTGT GTGTAGATGA CTGCTCCTCT 
AGCACCTGCA TGTGTGGCCA GCTGAGCATG CGCTGCTGGT ATGATAAGGA TGGCCGACTT 
CTGCCTGAGT TTAACATGGC AGAACCACCC TTGATCTTCG AGTGCAATCA TGCCTGCTCA 
TGCTGGAGGA ACTGCCGCAA TCGTGTGGTG CAAAATGGTC TCAGGGCAAG GCTGCAGCTT 
TATCGGACAC AGGACATGGG CTGGGGTGTG CGGTCCCTCC AGGATATCCC ACTGGGCACC 
TTTGTCTGCG AATACGTAGG GGAGCTGATT TCGGACTCTG AAGCTGATGT TCGGGAAGAG 
GACTCTTACC TCTTTGATCT TGACAATAAG GATGGAGAGG TATACTGCAT TGACGCTCGG 
TTCTATGGGA ATGTCAGCCG GTTCATAAAC CACCACTGCG AACCCAACCT TGTGCCTGTG 
CGAGTGTTCA TGTCACACCA GGACCTGCGG TTTCCCAGGA TTGCCTTCTT CAGTACCCGC 
CTGATTCAGG CTGGGGAGCA GCTCGGGTTC GACTACGGGG AGCGCTTTTG GGACGTCAAG 
GGCAAGCTCT TCAGTTGCCG GTGTGGGTCT TCCAAGTGTC GGCACTCAAG CGCAGCCCTG 
GCCCAGAGGC AAGCCAGTGC AGCCCAGGAG CCTCAGGAGA ATGGCCTTCC AGATACCAGC 
TCTGCAGCCG CTGCTGACCC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( NM_001012518.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001012536.2 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA.

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CloneID	OMu23314
Clone ID Related Accession (Same CDS sequence)	NM_001012518.3
Accession Version	NM_001012518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BB621957.1, BC089302.1 and DV649682.1. On Mar 9, 2013 this sequence version replaced NM_001012518.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082062.1, AB205007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTAT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGTTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAACA GAGGACGGTG ATTGAGATGT TTAAAAGCAT AACCCATTCC 
ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCAGA AGATGAAGAC TCCGATGAGC TGGAGGATGA CGAGGACCAT 
GGAGCTGAGC AAGCGGCTGC ATTTCCCACC GAGGATAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ACCGGGCTGC AAAGATGGAT GGAGATTCAG AGGAGGAACA GGAGTCTCCC 
GACACAGGGG AGGATGAAGA TGGTGGAGAC GAGTCTGACC TGAGTTCTGA ATCCAGTATC 
AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG ACTGACAGCC CCTGGATCAA ACCTGCTCGG 
AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG AGCAGCATGC TTGGTTCCGA GGCTTGTAAG 
TCATCTCCAG GAAGCATGGA GCAGGCAGCT CTGGGAGACA GCGCTGGCTA TATGGAAGTT 
TCCCTGGATT CCCTGGATCT CCGTGTCAGA GGAATTCTGT CTTCCCAGAC AGAAAATGAA 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAATTG GGCCGGTGTA CAAACAGCGT GGTCAAGTAT 
GAGCTGATGC GCCCATCCAA CAAAGCACCG CTCTTGGTGT TATGTGAAGA CCATCGGGGT 
CGCATGGTGA AGCACCAGTG CTGTCCTGGC TGCGGCTACT TCTGCACGGC GGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012536.2
CDS	36..3926
Translation	MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	NM_001012518.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012518.3

ORF Insert Sequence:

ATGGCCGCCG CTGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAGCTGCT AAGGGAAGAT ACACCTATGG CTGCTGATGA AGGTTCCACA 
GAGAAACAAG AAGGAGAGAC TCCCATGGCT GCAGATGGAG AAACAAATGG GTCTTGTGAA 
AAGAGTGGGG ATCCCAGCCA TCTAAATGCA CCCAAACACA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTGTCT CGGGTGGCAG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTC ACAGCTGACG ACTTCATGCA GACATCTGTC 
ATTGGCAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGGCAGCC GTTGAGGACT 
CCCAACATTC TAACCTCCTC GCTTCCTGGT CATGCTGCAA AAACTCTTCC TGGAGGAGCC 
AGTAAATGCA GGACTCTGAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCTTGG GCCTGCATGC AGCCAGCAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACC TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAGTGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTAT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGTTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAACA GAGGACGGTG ATTGAGATGT TTAAAAGCAT AACCCATTCC 
ACTGTGGGCG CCAAGGGCGA GAAAGCCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCAGA AGATGAAGAC TCCGATGAGC TGGAGGATGA CGAGGACCAT 
GGAGCTGAGC AAGCGGCTGC ATTTCCCACC GAGGATAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ACCGGGCTGC AAAGATGGAT GGAGATTCAG AGGAGGAACA GGAGTCTCCC 
GACACAGGGG AGGATGAAGA TGGTGGAGAC GAGTCTGACC TGAGTTCTGA ATCCAGTATC 
AAGAAGAAAT TTCTCAAGAG GAGAGGGAAG ACTGACAGCC CCTGGATCAA ACCTGCTCGG 
AAAAGGAGGC GGAGAAGTAG AAAGAAGCCG AGCAGCATGC TTGGTTCCGA GGCTTGTAAG 
TCATCTCCAG GAAGCATGGA GCAGGCAGCT CTGGGAGACA GCGCTGGCTA TATGGAAGTT 
TCCCTGGATT CCCTGGATCT CCGTGTCAGA GGAATTCTGT CTTCCCAGAC AGAAAATGAA 
GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG ACGGATGGCC TCCAGGAAGT GCCTCTCTGC 
AGCTGCCGAA TGGAAACCCC CAAGAGCCGC GAGATCAGCA CCCTGGCCAA CAACCAGTGC 
ATGGCCACTG AGAGCGTGGA TCACGAATTG GGCCGGTGTA CAAACAGCGT GGTCAAGTAT 
GAGCTGATGC GCCCATCCAA CAAAGCACCG CTCTTGGTGT TATGTGAAGA CCATCGGGGT 
CGCATGGTGA AGCACCAGTG CTGTCCTGGC TGCGGCTACT TCTGCACGGC GGGTAACTTC 
ATGGAATGCC AGCCTGAGAG CAGCATCTCT CATCGTTTCC ATAAGGACTG TGCCTCTCGA 
GTCAACAATG CCAGCTACTG TCCCCATTGT GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG 
ACCATAGCAA AAGCAGACAC AACTTCCACA GTGACCCTAG CCCCTGGACA GGAGAAGAGC 
CTGGCTGCTG AAGGCAGGGC TGACACGACC ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT 
GAAAGATCGC AGAGCACAGC CCCCCAGGCA CCAGAGTGCT TCGACCCTGC CGGACCGGCT 
GGGCTCGTGA GGCCGACATC TGGCCTTTCC CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT 
GCTCTAATCG CTCTAGACTC TGAAAAACCC AAGAAACTTC GCTTCCACCC AAAGCAGCTG 
TACTTCTCTG CCAGGCAGGG TGAGCTGCAG AAGGTGCTTC TCATGCTGGT TGATGGAATT 
GATCCCAACT TCAAAATGGA GCACCAAAGT AAGCGTTCCC CATTACATGC TGCTGCGGAG 
GCTGGCCACG TGGACATCTG CCACATGCTG GTTCAGGCGG GTGCCAATAT TGACACTTGC 
TCAGAGGACC AGCGGACCCC ACTGATGGAG GCTGCAGAGA ACAACCACTT GGATGCAGTG 
AAGTACCTCA TCAAGGCTGG AGCACAGGTG GATCCGAAGG ACGCAGAGGG CTCCACATGT 
TTGCATTTGG CTGCCAAGAA AGGCCACTAT GATGTGGTTC AGTATCTGCT TTCAAATGGA 
CAGATGGATG TCAACTGCCA GGATGACGGT GGATGGACAC CTATGATCTG GGCCACTGAG 
TACAAGCACG TGGAGCTGGT GAAGCTGCTG CTGTCTAAGG GCTCTGACAT CAACATCCGG 
GACAACGAGG AGAACATTTG TCTGCACTGG GCAGCATTTT CAGGCTGTGT GGACATAGCT 
GAAATACTTC TGGCTGCCAA GTGTGACCTG CATGCTGTGA ATATCCATGG AGACTCACCC 
CTGCACATCG CAGCCAGGGA GAATCGCTAC GACTGTGTTG TCCTCTTTCT TTCTCGGGAT 
TCAGATGTTA CTCTGAAAAA CAAGGAAGGA GAGACTCCCT TGCAGTGTGC AAGTCTCAGT 
TCGCAGGTGT GGAGTGCATT GCAGATGAGC AAAGCACTTC GGGACTCAGC CCCTGACAAG 
CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG GATATCGCTC GAGGGTATGA GCGCATTCCC 
ATTCCCTGTG TCAATGCTGT GGACAGTGAG CTGTGTCCTA CCAACTATAA GTATGTCTCC 
CAGAACTGTG TGACATCCCC CATGAACATT GACAGGAACA TCACTCATTT GCAGTACTGC 
GTGTGTGTAG ATGACTGCTC CTCTAGCACC TGCATGTGTG GCCAGCTGAG CATGCGCTGC 
TGGTATGATA AGGATGGCCG ACTTCTGCCT GAGTTTAACA TGGCAGAACC ACCCTTGATC 
TTCGAGTGCA ATCATGCCTG CTCATGCTGG AGGAACTGCC GCAATCGTGT GGTGCAAAAT 
GGTCTCAGGG CAAGGCTGCA GCTTTATCGG ACACAGGACA TGGGCTGGGG TGTGCGGTCC 
CTCCAGGATA TCCCACTGGG CACCTTTGTC TGCGAATACG TAGGGGAGCT GATTTCGGAC 
TCTGAAGCTG ATGTTCGGGA AGAGGACTCT TACCTCTTTG ATCTTGACAA TAAGGATGGA 
GAGGTATACT GCATTGACGC TCGGTTCTAT GGGAATGTCA GCCGGTTCAT AAACCACCAC 
TGCGAACCCA ACCTTGTGCC TGTGCGAGTG TTCATGTCAC ACCAGGACCT GCGGTTTCCC 
AGGATTGCCT TCTTCAGTAC CCGCCTGATT CAGGCTGGGG AGCAGCTCGG GTTCGACTAC 
GGGGAGCGCT TTTGGGACGT CAAGGGCAAG CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG 
TGTCGGCACT CAAGCGCAGC CCTGGCCCAG AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG 
GAGAATGGCC TTCCAGATAC CAGCTCTGCA GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498422.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498485.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.

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CloneID	OMu29800
Clone ID Related Accession (Same CDS sequence)	XM_006498422.2
Accession Version	XM_006498422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA 
AGCATGGAGC AGGCAGCTCT GGGAGACAGC GCTGGCTATA TGGAAGTTTC CCTGGATTCC 
CTGGATCTCC GTGTCAGAGG AATTCTGTCT TCCCAGACAG AAAATGAAGG GCTGGCCAGT 
GGTCCGGATG TGCTGGGGAC GGATGGCCTC CAGGAAGTGC CTCTCTGCAG CTGCCGAATG 
GAAACCCCCA AGAGCCGCGA GATCAGCACC CTGGCCAACA ACCAGTGCAT GGCCACTGAG 
AGCGTGGATC ACGAATTGGG CCGGTGTACA AACAGCGTGG TCAAGTATGA GCTGATGCGC 
CCATCCAACA AAGCACCGCT CTTGGTGTTA TGTGAAGACC ATCGGGGTCG CATGGTGAAG 
CACCAGTGCT GTCCTGGCTG CGGCTACTTC TGCACGGCGG GTAACTTCAT GGAATGCCAG 
CCTGAGAGCA GCATCTCTCA TCGTTTCCAT AAGGACTGTG CCTCTCGAGT CAACAATGCC 
AGCTACTGTC CCCATTGTGG GGAAGAAGCT TCCAAGGCCA AAGAGGTGAC CATAGCAAAA 
GCAGACACAA CTTCCACAGT GACCCTAGCC CCTGGACAGG AGAAGAGCCT GGCTGCTGAA 
GGCAGGGCTG ACACGACCAC GGGCAGCATT GCTGGAGCCC CAGAGGATGA AAGATCGCAG 
AGCACAGCCC CCCAGGCACC AGAGTGCTTC GACCCTGCCG GACCGGCTGG GCTCGTGAGG 
CCGACATCTG GCCTTTCCCA GGGCCCAGGA AAGGAAACCT TGGAAAGTGC TCTAATCGCT 
CTAGACTCTG AAAAACCCAA GAAACTTCGC TTCCACCCAA AGCAGCTGTA CTTCTCTGCC 
AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC ATGCTGGTTG ATGGAATTGA TCCCAACTTC 
AAAATGGAGC ACCAAAGTAA GCGTTCCCCA TTACATGCTG CTGCGGAGGC TGGCCACGTG 
GACATCTGCC ACATGCTGGT TCAGGCGGGT GCCAATATTG ACACTTGCTC AGAGGACCAG 
CGGACCCCAC TGATGGAGGC TGCAGAGAAC AACCACTTGG ATGCAGTGAA GTACCTCATC 
AAGGCTGGAG CACAGGTGGA TCCGAAGGAC GCAGAGGGCT CCACATGTTT GCATTTGGCT 
GCCAAGAAAG GCCACTATGA TGTGGTTCAG TATCTGCTTT CAAATGGACA GATGGATGTC 
AACTGCCAGG ATGACGGTGG ATGGACACCT ATGATCTGGG CCACTGAGTA CAAGCACGTG 
GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC TCTGACATCA ACATCCGGGA CAACGAGGAG 
AACATTTGTC TGCACTGGGC AGCATTTTCA GGCTGTGTGG ACATAGCTGA AATACTTCTG 
GCTGCCAAGT GTGACCTGCA TGCTGTGAAT ATCCATGGAG ACTCACCCCT GCACATCGCA 
GCCAGGGAGA ATCGCTACGA CTGTGTTGTC CTCTTTCTTT CTCGGGATTC AGATGTTACT 
CTGAAAAACA AGGAAGGAGA GACTCCCTTG CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG 
AGTGCATTGC AGATGAGCAA AGCACTTCGG GACTCAGCCC CTGACAAGCC CGTTGCTGTT 
GAGAAGACGG TGAGCAGGGA TATCGCTCGA GGGTATGAGC GCATTCCCAT TCCCTGTGTC 
AATGCTGTGG ACAGTGAGCT GTGTCCTACC AACTATAAGT ATGTCTCCCA GAACTGTGTG 
ACATCCCCCA TGAACATTGA CAGGAACATC ACTCATTTGC AGTACTGCGT GTGTGTAGAT 
GACTGCTCCT CTAGCACCTG CATGTGTGGC CAGCTGAGCA TGCGCTGCTG GTATGATAAG 
GATGGCCGAC TTCTGCCTGA GTTTAACATG GCAGAACCAC CCTTGATCTT CGAGTGCAAT 
CATGCCTGCT CATGCTGGAG GAACTGCCGC AATCGTGTGG TGCAAAATGG TCTCAGGGCA 
AGGCTGCAGC TTTATCGGAC ACAGGACATG GGCTGGGGTG TGCGGTCCCT CCAGGATATC 
CCACTGGGCA CCTTTGTCTG CGAATACGTA GGGGAGCTGA TTTCGGACTC TGAAGCTGAT 
GTTCGGGAAG AGGACTCTTA CCTCTTTGAT CTTGACAATA AGGATGGAGA GGTATACTGC 
ATTGACGCTC GGTTCTATGG GAATGTCAGC CGGTTCATAA ACCACCACTG CGAACCCAAC 
CTTGTGCCTG TGCGAGTGTT CATGTCACAC CAGGACCTGC GGTTTCCCAG GATTGCCTTC 
TTCAGTACCC GCCTGATTCA GGCTGGGGAG CAGCTCGGGT TCGACTACGG GGAGCGCTTT 
TGGGACGTCA AGGGCAAGCT CTTCAGTTGC CGGTGTGGGT CTTCCAAGTG TCGGCACTCA 
AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT GCAGCCCAGG AGCCTCAGGA GAATGGCCTT 
CCAGATACCA GCTCTGCAGC CGCTGCTGAC CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498485.1
CDS	27..3965
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498422.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498422.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA 
AGCATGGAGC AGGCAGCTCT GGGAGACAGC GCTGGCTATA TGGAAGTTTC CCTGGATTCC 
CTGGATCTCC GTGTCAGAGG AATTCTGTCT TCCCAGACAG AAAATGAAGG GCTGGCCAGT 
GGTCCGGATG TGCTGGGGAC GGATGGCCTC CAGGAAGTGC CTCTCTGCAG CTGCCGAATG 
GAAACCCCCA AGAGCCGCGA GATCAGCACC CTGGCCAACA ACCAGTGCAT GGCCACTGAG 
AGCGTGGATC ACGAATTGGG CCGGTGTACA AACAGCGTGG TCAAGTATGA GCTGATGCGC 
CCATCCAACA AAGCACCGCT CTTGGTGTTA TGTGAAGACC ATCGGGGTCG CATGGTGAAG 
CACCAGTGCT GTCCTGGCTG CGGCTACTTC TGCACGGCGG GTAACTTCAT GGAATGCCAG 
CCTGAGAGCA GCATCTCTCA TCGTTTCCAT AAGGACTGTG CCTCTCGAGT CAACAATGCC 
AGCTACTGTC CCCATTGTGG GGAAGAAGCT TCCAAGGCCA AAGAGGTGAC CATAGCAAAA 
GCAGACACAA CTTCCACAGT GACCCTAGCC CCTGGACAGG AGAAGAGCCT GGCTGCTGAA 
GGCAGGGCTG ACACGACCAC GGGCAGCATT GCTGGAGCCC CAGAGGATGA AAGATCGCAG 
AGCACAGCCC CCCAGGCACC AGAGTGCTTC GACCCTGCCG GACCGGCTGG GCTCGTGAGG 
CCGACATCTG GCCTTTCCCA GGGCCCAGGA AAGGAAACCT TGGAAAGTGC TCTAATCGCT 
CTAGACTCTG AAAAACCCAA GAAACTTCGC TTCCACCCAA AGCAGCTGTA CTTCTCTGCC 
AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC ATGCTGGTTG ATGGAATTGA TCCCAACTTC 
AAAATGGAGC ACCAAAGTAA GCGTTCCCCA TTACATGCTG CTGCGGAGGC TGGCCACGTG 
GACATCTGCC ACATGCTGGT TCAGGCGGGT GCCAATATTG ACACTTGCTC AGAGGACCAG 
CGGACCCCAC TGATGGAGGC TGCAGAGAAC AACCACTTGG ATGCAGTGAA GTACCTCATC 
AAGGCTGGAG CACAGGTGGA TCCGAAGGAC GCAGAGGGCT CCACATGTTT GCATTTGGCT 
GCCAAGAAAG GCCACTATGA TGTGGTTCAG TATCTGCTTT CAAATGGACA GATGGATGTC 
AACTGCCAGG ATGACGGTGG ATGGACACCT ATGATCTGGG CCACTGAGTA CAAGCACGTG 
GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC TCTGACATCA ACATCCGGGA CAACGAGGAG 
AACATTTGTC TGCACTGGGC AGCATTTTCA GGCTGTGTGG ACATAGCTGA AATACTTCTG 
GCTGCCAAGT GTGACCTGCA TGCTGTGAAT ATCCATGGAG ACTCACCCCT GCACATCGCA 
GCCAGGGAGA ATCGCTACGA CTGTGTTGTC CTCTTTCTTT CTCGGGATTC AGATGTTACT 
CTGAAAAACA AGGAAGGAGA GACTCCCTTG CAGTGTGCAA GTCTCAGTTC GCAGGTGTGG 
AGTGCATTGC AGATGAGCAA AGCACTTCGG GACTCAGCCC CTGACAAGCC CGTTGCTGTT 
GAGAAGACGG TGAGCAGGGA TATCGCTCGA GGGTATGAGC GCATTCCCAT TCCCTGTGTC 
AATGCTGTGG ACAGTGAGCT GTGTCCTACC AACTATAAGT ATGTCTCCCA GAACTGTGTG 
ACATCCCCCA TGAACATTGA CAGGAACATC ACTCATTTGC AGTACTGCGT GTGTGTAGAT 
GACTGCTCCT CTAGCACCTG CATGTGTGGC CAGCTGAGCA TGCGCTGCTG GTATGATAAG 
GATGGCCGAC TTCTGCCTGA GTTTAACATG GCAGAACCAC CCTTGATCTT CGAGTGCAAT 
CATGCCTGCT CATGCTGGAG GAACTGCCGC AATCGTGTGG TGCAAAATGG TCTCAGGGCA 
AGGCTGCAGC TTTATCGGAC ACAGGACATG GGCTGGGGTG TGCGGTCCCT CCAGGATATC 
CCACTGGGCA CCTTTGTCTG CGAATACGTA GGGGAGCTGA TTTCGGACTC TGAAGCTGAT 
GTTCGGGAAG AGGACTCTTA CCTCTTTGAT CTTGACAATA AGGATGGAGA GGTATACTGC 
ATTGACGCTC GGTTCTATGG GAATGTCAGC CGGTTCATAA ACCACCACTG CGAACCCAAC 
CTTGTGCCTG TGCGAGTGTT CATGTCACAC CAGGACCTGC GGTTTCCCAG GATTGCCTTC 
TTCAGTACCC GCCTGATTCA GGCTGGGGAG CAGCTCGGGT TCGACTACGG GGAGCGCTTT 
TGGGACGTCA AGGGCAAGCT CTTCAGTTGC CGGTGTGGGT CTTCCAAGTG TCGGCACTCA 
AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT GCAGCCCAGG AGCCTCAGGA GAATGGCCTT 
CCAGATACCA GCTCTGCAGC CGCTGCTGAC CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498486.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29801
Clone ID Related Accession (Same CDS sequence)	XM_006498423.2
Accession Version	XM_006498423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTTGCTTG CCTTTTGTTT TAGCAGCTGC AGTATCTCGG 
AAGAAAAAAC GAAGAATGGG AACCTATAGT TTAGTTCCCA AGAAAAAGAC CAAAGTATTA 
AAACAGAGGA CGGTGATTGA GATGTTTAAA AGCATAACCC ATTCCACTGT GGGCGCCAAG 
GGCGAGAAAG CCTTAGATGA TAGTGCCCTG CATGTAAATG GCGAGAGCTT GGAGATGGAC 
TCAGAAGATG AAGACTCCGA TGAGCTGGAG GATGACGAGG ACCATGGAGC TGAGCAAGCG 
GCTGCATTTC CCACCGAGGA TAGTAGGACT TCTAAAGAGA GCATGTCTGA GACTGACCGG 
GCTGCAAAGA TGGATGGAGA TTCAGAGGAG GAACAGGAGT CTCCCGACAC AGGGGAGGAT 
GAAGATGGTG GAGACGAGTC TGACCTGAGT TCTGAATCCA GTATCAAGAA GAAATTTCTC 
AAGAGGAGAG GGAAGACTGA CAGCCCCTGG ATCAAACCTG CTCGGAAAAG GAGGCGGAGA 
AGTAGAAAGA AGCCGAGCAG CATGCTTGGT TCCGAGGCTT GTAAGTCATC TCCAGGAAGC 
ATGGAGCAGG CAGCTCTGGG AGACAGCGCT GGCTATATGG AAGTTTCCCT GGATTCCCTG 
GATCTCCGTG TCAGAGGAAT TCTGTCTTCC CAGACAGAAA ATGAAGGGCT GGCCAGTGGT 
CCGGATGTGC TGGGGACGGA TGGCCTCCAG GAAGTGCCTC TCTGCAGCTG CCGAATGGAA 
ACCCCCAAGA GCCGCGAGAT CAGCACCCTG GCCAACAACC AGTGCATGGC CACTGAGAGC 
GTGGATCACG AATTGGGCCG GTGTACAAAC AGCGTGGTCA AGTATGAGCT GATGCGCCCA 
TCCAACAAAG CACCGCTCTT GGTGTTATGT GAAGACCATC GGGGTCGCAT GGTGAAGCAC 
CAGTGCTGTC CTGGCTGCGG CTACTTCTGC ACGGCGGGTA ACTTCATGGA ATGCCAGCCT 
GAGAGCAGCA TCTCTCATCG TTTCCATAAG GACTGTGCCT CTCGAGTCAA CAATGCCAGC 
TACTGTCCCC ATTGTGGGGA AGAAGCTTCC AAGGCCAAAG AGGTGACCAT AGCAAAAGCA 
GACACAACTT CCACAGTGAC CCTAGCCCCT GGACAGGAGA AGAGCCTGGC TGCTGAAGGC 
AGGGCTGACA CGACCACGGG CAGCATTGCT GGAGCCCCAG AGGATGAAAG ATCGCAGAGC 
ACAGCCCCCC AGGCACCAGA GTGCTTCGAC CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG 
ACATCTGGCC TTTCCCAGGG CCCAGGAAAG GAAACCTTGG AAAGTGCTCT AATCGCTCTA 
GACTCTGAAA AACCCAAGAA ACTTCGCTTC CACCCAAAGC AGCTGTACTT CTCTGCCAGG 
CAGGGTGAGC TGCAGAAGGT GCTTCTCATG CTGGTTGATG GAATTGATCC CAACTTCAAA 
ATGGAGCACC AAAGTAAGCG TTCCCCATTA CATGCTGCTG CGGAGGCTGG CCACGTGGAC 
ATCTGCCACA TGCTGGTTCA GGCGGGTGCC AATATTGACA CTTGCTCAGA GGACCAGCGG 
ACCCCACTGA TGGAGGCTGC AGAGAACAAC CACTTGGATG CAGTGAAGTA CCTCATCAAG 
GCTGGAGCAC AGGTGGATCC GAAGGACGCA GAGGGCTCCA CATGTTTGCA TTTGGCTGCC 
AAGAAAGGCC ACTATGATGT GGTTCAGTAT CTGCTTTCAA ATGGACAGAT GGATGTCAAC 
TGCCAGGATG ACGGTGGATG GACACCTATG ATCTGGGCCA CTGAGTACAA GCACGTGGAG 
CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT GACATCAACA TCCGGGACAA CGAGGAGAAC 
ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC TGTGTGGACA TAGCTGAAAT ACTTCTGGCT 
GCCAAGTGTG ACCTGCATGC TGTGAATATC CATGGAGACT CACCCCTGCA CATCGCAGCC 
AGGGAGAATC GCTACGACTG TGTTGTCCTC TTTCTTTCTC GGGATTCAGA TGTTACTCTG 
AAAAACAAGG AAGGAGAGAC TCCCTTGCAG TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT 
GCATTGCAGA TGAGCAAAGC ACTTCGGGAC TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG 
AAGACGGTGA GCAGGGATAT CGCTCGAGGG TATGAGCGCA TTCCCATTCC CTGTGTCAAT 
GCTGTGGACA GTGAGCTGTG TCCTACCAAC TATAAGTATG TCTCCCAGAA CTGTGTGACA 
TCCCCCATGA ACATTGACAG GAACATCACT CATTTGCAGT ACTGCGTGTG TGTAGATGAC 
TGCTCCTCTA GCACCTGCAT GTGTGGCCAG CTGAGCATGC GCTGCTGGTA TGATAAGGAT 
GGCCGACTTC TGCCTGAGTT TAACATGGCA GAACCACCCT TGATCTTCGA GTGCAATCAT 
GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT CGTGTGGTGC AAAATGGTCT CAGGGCAAGG 
CTGCAGCTTT ATCGGACACA GGACATGGGC TGGGGTGTGC GGTCCCTCCA GGATATCCCA 
CTGGGCACCT TTGTCTGCGA ATACGTAGGG GAGCTGATTT CGGACTCTGA AGCTGATGTT 
CGGGAAGAGG ACTCTTACCT CTTTGATCTT GACAATAAGG ATGGAGAGGT ATACTGCATT 
GACGCTCGGT TCTATGGGAA TGTCAGCCGG TTCATAAACC ACCACTGCGA ACCCAACCTT 
GTGCCTGTGC GAGTGTTCAT GTCACACCAG GACCTGCGGT TTCCCAGGAT TGCCTTCTTC 
AGTACCCGCC TGATTCAGGC TGGGGAGCAG CTCGGGTTCG ACTACGGGGA GCGCTTTTGG 
GACGTCAAGG GCAAGCTCTT CAGTTGCCGG TGTGGGTCTT CCAAGTGTCG GCACTCAAGC 
GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA 
GATACCAGCT CTGCAGCCGC TGCTGACCCC CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498486.1
CDS	18..3953
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498423.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGGTA AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTTGCTTG CCTTTTGTTT TAGCAGCTGC AGTATCTCGG 
AAGAAAAAAC GAAGAATGGG AACCTATAGT TTAGTTCCCA AGAAAAAGAC CAAAGTATTA 
AAACAGAGGA CGGTGATTGA GATGTTTAAA AGCATAACCC ATTCCACTGT GGGCGCCAAG 
GGCGAGAAAG CCTTAGATGA TAGTGCCCTG CATGTAAATG GCGAGAGCTT GGAGATGGAC 
TCAGAAGATG AAGACTCCGA TGAGCTGGAG GATGACGAGG ACCATGGAGC TGAGCAAGCG 
GCTGCATTTC CCACCGAGGA TAGTAGGACT TCTAAAGAGA GCATGTCTGA GACTGACCGG 
GCTGCAAAGA TGGATGGAGA TTCAGAGGAG GAACAGGAGT CTCCCGACAC AGGGGAGGAT 
GAAGATGGTG GAGACGAGTC TGACCTGAGT TCTGAATCCA GTATCAAGAA GAAATTTCTC 
AAGAGGAGAG GGAAGACTGA CAGCCCCTGG ATCAAACCTG CTCGGAAAAG GAGGCGGAGA 
AGTAGAAAGA AGCCGAGCAG CATGCTTGGT TCCGAGGCTT GTAAGTCATC TCCAGGAAGC 
ATGGAGCAGG CAGCTCTGGG AGACAGCGCT GGCTATATGG AAGTTTCCCT GGATTCCCTG 
GATCTCCGTG TCAGAGGAAT TCTGTCTTCC CAGACAGAAA ATGAAGGGCT GGCCAGTGGT 
CCGGATGTGC TGGGGACGGA TGGCCTCCAG GAAGTGCCTC TCTGCAGCTG CCGAATGGAA 
ACCCCCAAGA GCCGCGAGAT CAGCACCCTG GCCAACAACC AGTGCATGGC CACTGAGAGC 
GTGGATCACG AATTGGGCCG GTGTACAAAC AGCGTGGTCA AGTATGAGCT GATGCGCCCA 
TCCAACAAAG CACCGCTCTT GGTGTTATGT GAAGACCATC GGGGTCGCAT GGTGAAGCAC 
CAGTGCTGTC CTGGCTGCGG CTACTTCTGC ACGGCGGGTA ACTTCATGGA ATGCCAGCCT 
GAGAGCAGCA TCTCTCATCG TTTCCATAAG GACTGTGCCT CTCGAGTCAA CAATGCCAGC 
TACTGTCCCC ATTGTGGGGA AGAAGCTTCC AAGGCCAAAG AGGTGACCAT AGCAAAAGCA 
GACACAACTT CCACAGTGAC CCTAGCCCCT GGACAGGAGA AGAGCCTGGC TGCTGAAGGC 
AGGGCTGACA CGACCACGGG CAGCATTGCT GGAGCCCCAG AGGATGAAAG ATCGCAGAGC 
ACAGCCCCCC AGGCACCAGA GTGCTTCGAC CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG 
ACATCTGGCC TTTCCCAGGG CCCAGGAAAG GAAACCTTGG AAAGTGCTCT AATCGCTCTA 
GACTCTGAAA AACCCAAGAA ACTTCGCTTC CACCCAAAGC AGCTGTACTT CTCTGCCAGG 
CAGGGTGAGC TGCAGAAGGT GCTTCTCATG CTGGTTGATG GAATTGATCC CAACTTCAAA 
ATGGAGCACC AAAGTAAGCG TTCCCCATTA CATGCTGCTG CGGAGGCTGG CCACGTGGAC 
ATCTGCCACA TGCTGGTTCA GGCGGGTGCC AATATTGACA CTTGCTCAGA GGACCAGCGG 
ACCCCACTGA TGGAGGCTGC AGAGAACAAC CACTTGGATG CAGTGAAGTA CCTCATCAAG 
GCTGGAGCAC AGGTGGATCC GAAGGACGCA GAGGGCTCCA CATGTTTGCA TTTGGCTGCC 
AAGAAAGGCC ACTATGATGT GGTTCAGTAT CTGCTTTCAA ATGGACAGAT GGATGTCAAC 
TGCCAGGATG ACGGTGGATG GACACCTATG ATCTGGGCCA CTGAGTACAA GCACGTGGAG 
CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT GACATCAACA TCCGGGACAA CGAGGAGAAC 
ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC TGTGTGGACA TAGCTGAAAT ACTTCTGGCT 
GCCAAGTGTG ACCTGCATGC TGTGAATATC CATGGAGACT CACCCCTGCA CATCGCAGCC 
AGGGAGAATC GCTACGACTG TGTTGTCCTC TTTCTTTCTC GGGATTCAGA TGTTACTCTG 
AAAAACAAGG AAGGAGAGAC TCCCTTGCAG TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT 
GCATTGCAGA TGAGCAAAGC ACTTCGGGAC TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG 
AAGACGGTGA GCAGGGATAT CGCTCGAGGG TATGAGCGCA TTCCCATTCC CTGTGTCAAT 
GCTGTGGACA GTGAGCTGTG TCCTACCAAC TATAAGTATG TCTCCCAGAA CTGTGTGACA 
TCCCCCATGA ACATTGACAG GAACATCACT CATTTGCAGT ACTGCGTGTG TGTAGATGAC 
TGCTCCTCTA GCACCTGCAT GTGTGGCCAG CTGAGCATGC GCTGCTGGTA TGATAAGGAT 
GGCCGACTTC TGCCTGAGTT TAACATGGCA GAACCACCCT TGATCTTCGA GTGCAATCAT 
GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT CGTGTGGTGC AAAATGGTCT CAGGGCAAGG 
CTGCAGCTTT ATCGGACACA GGACATGGGC TGGGGTGTGC GGTCCCTCCA GGATATCCCA 
CTGGGCACCT TTGTCTGCGA ATACGTAGGG GAGCTGATTT CGGACTCTGA AGCTGATGTT 
CGGGAAGAGG ACTCTTACCT CTTTGATCTT GACAATAAGG ATGGAGAGGT ATACTGCATT 
GACGCTCGGT TCTATGGGAA TGTCAGCCGG TTCATAAACC ACCACTGCGA ACCCAACCTT 
GTGCCTGTGC GAGTGTTCAT GTCACACCAG GACCTGCGGT TTCCCAGGAT TGCCTTCTTC 
AGTACCCGCC TGATTCAGGC TGGGGAGCAG CTCGGGTTCG ACTACGGGGA GCGCTTTTGG 
GACGTCAAGG GCAAGCTCTT CAGTTGCCGG TGTGGGTCTT CCAAGTGTCG GCACTCAAGC 
GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA 
GATACCAGCT CTGCAGCCGC TGCTGACCCC CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498424.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498487.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.

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CloneID	OMu29802
Clone ID Related Accession (Same CDS sequence)	XM_006498424.2
Accession Version	XM_006498424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498487.1
CDS	18..3947
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498424.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498424.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAGCTGCTA 
AGGGAAGATA CACCTATGGC TGCTGATGAA GGTTCCACAG AGAAACAAGA AGGAGAGACT 
CCCATGGCTG CAGATGGAGA AACAAATGGG TCTTGTGAAA AGAGTGGGGA TCCCAGCCAT 
CTAAATGCAC CCAAACACAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTGTCTC GGGTGGCAGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTCA CAGCTGACGA CTTCATGCAG ACATCTGTCA TTGGCAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGGCAGCCG TTGAGGACTC CCAACATTCT AACCTCCTCG 
CTTCCTGGTC ATGCTGCAAA AACTCTTCCT GGAGGAGCCA GTAAATGCAG GACTCTGAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCTTGGG CCTGCATGCA GCCAGCAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACCT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAGTGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTATC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGTTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAACAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATA ACCCATTCCA CTGTGGGCGC CAAGGGCGAG 
AAAGCCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCAGAA 
GATGAAGACT CCGATGAGCT GGAGGATGAC GAGGACCATG GAGCTGAGCA AGCGGCTGCA 
TTTCCCACCG AGGATAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA CCGGGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCCG ACACAGGGGA GGATGAAGAT 
GGTGGAGACG AGTCTGACCT GAGTTCTGAA TCCAGTATCA AGAAGAAATT TCTCAAGAGG 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGTAGA 
AAGAAGCCGA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCTCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGCTAT ATGGAAGTTT CCCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACA GAAAATGAAG GGCTGGCCAG TGGTCCGGAT 
GTGCTGGGGA CGGATGGCCT CCAGGAAGTG CCTCTCTGCA GCTGCCGAAT GGAAACCCCC 
AAGAGCCGCG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCATCCAAC 
AAAGCACCGC TCTTGGTGTT ATGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGCCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAAGC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACA 
ACTTCCACAG TGACCCTAGC CCCTGGACAG GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT 
GACACGACCA CGGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAGATCGCA GAGCACAGCC 
CCCCAGGCAC CAGAGTGCTT CGACCCTGCC GGACCGGCTG GGCTCGTGAG GCCGACATCT 
GGCCTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTAATCGC TCTAGACTCT 
GAAAAACCCA AGAAACTTCG CTTCCACCCA AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACATGCT GCTGCGGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GCGGACCCCA 
CTGATGGAGG CTGCAGAGAA CAACCACTTG GATGCAGTGA AGTACCTCAT CAAGGCTGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACATGTT TGCATTTGGC TGCCAAGAAA 
GGCCACTATG ATGTGGTTCA GTATCTGCTT TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGACGGTG GATGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGAGCTGGTG 
AAGCTGCTGC TGTCTAAGGG CTCTGACATC AACATCCGGG ACAACGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATACTTCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCATGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TCTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGTCTCAGTT CGCAGGTGTG GAGTGCATTG 
CAGATGAGCA AAGCACTTCG GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGGG ATATCGCTCG AGGGTATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GACAGTGAGC TGTGTCCTAC CAACTATAAG TATGTCTCCC AGAACTGTGT GACATCCCCC 
ATGAACATTG ACAGGAACAT CACTCATTTG CAGTACTGCG TGTGTGTAGA TGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGATAA GGATGGCCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCATGCTGGA GGAACTGCCG CAATCGTGTG GTGCAAAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TCCAGGATAT CCCACTGGGC 
ACCTTTGTCT GCGAATACGT AGGGGAGCTG ATTTCGGACT CTGAAGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTATACTG CATTGACGCT 
CGGTTCTATG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAACCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTG CGGTTTCCCA GGATTGCCTT CTTCAGTACC 
CGCCTGATTC AGGCTGGGGA GCAGCTCGGG TTCGACTACG GGGAGCGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTTCCAAGT GTCGGCACTC AAGCGCAGCC 
CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG GAGCCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498425.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498488.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu29803
Clone ID Related Accession (Same CDS sequence)	XM_006498425.2
Accession Version	XM_006498425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAATTGGGC 
CGGTGTACAA ACAGCGTGGT CAAGTATGAG CTGATGCGCC CATCCAACAA AGCACCGCTC 
TTGGTGTTAT GTGAAGACCA TCGGGGTCGC ATGGTGAAGC ACCAGTGCTG TCCTGGCTGC 
GGCTACTTCT GCACGGCGGG TAACTTCATG GAATGCCAGC CTGAGAGCAG CATCTCTCAT 
CGTTTCCATA AGGACTGTGC CTCTCGAGTC AACAATGCCA GCTACTGTCC CCATTGTGGG 
GAAGAAGCTT CCAAGGCCAA AGAGGTGACC ATAGCAAAAG CAGACACAAC TTCCACAGTG 
ACCCTAGCCC CTGGACAGGA GAAGAGCCTG GCTGCTGAAG GCAGGGCTGA CACGACCACG 
GGCAGCATTG CTGGAGCCCC AGAGGATGAA AGATCGCAGA GCACAGCCCC CCAGGCACCA 
GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG CTCGTGAGGC CGACATCTGG CCTTTCCCAG 
GGCCCAGGAA AGGAAACCTT GGAAAGTGCT CTAATCGCTC TAGACTCTGA AAAACCCAAG 
AAACTTCGCT TCCACCCAAA GCAGCTGTAC TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG 
GTGCTTCTCA TGCTGGTTGA TGGAATTGAT CCCAACTTCA AAATGGAGCA CCAAAGTAAG 
CGTTCCCCAT TACATGCTGC TGCGGAGGCT GGCCACGTGG ACATCTGCCA CATGCTGGTT 
CAGGCGGGTG CCAATATTGA CACTTGCTCA GAGGACCAGC GGACCCCACT GATGGAGGCT 
GCAGAGAACA ACCACTTGGA TGCAGTGAAG TACCTCATCA AGGCTGGAGC ACAGGTGGAT 
CCGAAGGACG CAGAGGGCTC CACATGTTTG CATTTGGCTG CCAAGAAAGG CCACTATGAT 
GTGGTTCAGT ATCTGCTTTC AAATGGACAG ATGGATGTCA ACTGCCAGGA TGACGGTGGA 
TGGACACCTA TGATCTGGGC CACTGAGTAC AAGCACGTGG AGCTGGTGAA GCTGCTGCTG 
TCTAAGGGCT CTGACATCAA CATCCGGGAC AACGAGGAGA ACATTTGTCT GCACTGGGCA 
GCATTTTCAG GCTGTGTGGA CATAGCTGAA ATACTTCTGG CTGCCAAGTG TGACCTGCAT 
GCTGTGAATA TCCATGGAGA CTCACCCCTG CACATCGCAG CCAGGGAGAA TCGCTACGAC 
TGTGTTGTCC TCTTTCTTTC TCGGGATTCA GATGTTACTC TGAAAAACAA GGAAGGAGAG 
ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG CAGGTGTGGA GTGCATTGCA GATGAGCAAA 
GCACTTCGGG ACTCAGCCCC TGACAAGCCC GTTGCTGTTG AGAAGACGGT GAGCAGGGAT 
ATCGCTCGAG GGTATGAGCG CATTCCCATT CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG 
TGTCCTACCA ACTATAAGTA TGTCTCCCAG AACTGTGTGA CATCCCCCAT GAACATTGAC 
AGGAACATCA CTCATTTGCA GTACTGCGTG TGTGTAGATG ACTGCTCCTC TAGCACCTGC 
ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG TATGATAAGG ATGGCCGACT TCTGCCTGAG 
TTTAACATGG CAGAACCACC CTTGATCTTC GAGTGCAATC ATGCCTGCTC ATGCTGGAGG 
AACTGCCGCA ATCGTGTGGT GCAAAATGGT CTCAGGGCAA GGCTGCAGCT TTATCGGACA 
CAGGACATGG GCTGGGGTGT GCGGTCCCTC CAGGATATCC CACTGGGCAC CTTTGTCTGC 
GAATACGTAG GGGAGCTGAT TTCGGACTCT GAAGCTGATG TTCGGGAAGA GGACTCTTAC 
CTCTTTGATC TTGACAATAA GGATGGAGAG GTATACTGCA TTGACGCTCG GTTCTATGGG 
AATGTCAGCC GGTTCATAAA CCACCACTGC GAACCCAACC TTGTGCCTGT GCGAGTGTTC 
ATGTCACACC AGGACCTGCG GTTTCCCAGG ATTGCCTTCT TCAGTACCCG CCTGATTCAG 
GCTGGGGAGC AGCTCGGGTT CGACTACGGG GAGCGCTTTT GGGACGTCAA GGGCAAGCTC 
TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT CGGCACTCAA GCGCAGCCCT GGCCCAGAGG 
CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG AATGGCCTTC CAGATACCAG CTCTGCAGCC 
GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498488.1
CDS	27..3944
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498425.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498425.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GTTCCGAGGC TTGTAAGTCA TCTCCAGGAA GCATGGAGCA GGCAGCTCTG 
GGAGACAGCG CTGGCTATAT GGAAGTTTCC CTGGATTCCC TGGATCTCCG TGTCAGAGGA 
ATTCTGTCTT CCCAGACAGA AAATGAAGGG CTGGCCAGTG GTCCGGATGT GCTGGGGACG 
GATGGCCTCC AGGAAGTGCC TCTCTGCAGC TGCCGAATGG AAACCCCCAA GAGCCGCGAG 
ATCAGCACCC TGGCCAACAA CCAGTGCATG GCCACTGAGA GCGTGGATCA CGAATTGGGC 
CGGTGTACAA ACAGCGTGGT CAAGTATGAG CTGATGCGCC CATCCAACAA AGCACCGCTC 
TTGGTGTTAT GTGAAGACCA TCGGGGTCGC ATGGTGAAGC ACCAGTGCTG TCCTGGCTGC 
GGCTACTTCT GCACGGCGGG TAACTTCATG GAATGCCAGC CTGAGAGCAG CATCTCTCAT 
CGTTTCCATA AGGACTGTGC CTCTCGAGTC AACAATGCCA GCTACTGTCC CCATTGTGGG 
GAAGAAGCTT CCAAGGCCAA AGAGGTGACC ATAGCAAAAG CAGACACAAC TTCCACAGTG 
ACCCTAGCCC CTGGACAGGA GAAGAGCCTG GCTGCTGAAG GCAGGGCTGA CACGACCACG 
GGCAGCATTG CTGGAGCCCC AGAGGATGAA AGATCGCAGA GCACAGCCCC CCAGGCACCA 
GAGTGCTTCG ACCCTGCCGG ACCGGCTGGG CTCGTGAGGC CGACATCTGG CCTTTCCCAG 
GGCCCAGGAA AGGAAACCTT GGAAAGTGCT CTAATCGCTC TAGACTCTGA AAAACCCAAG 
AAACTTCGCT TCCACCCAAA GCAGCTGTAC TTCTCTGCCA GGCAGGGTGA GCTGCAGAAG 
GTGCTTCTCA TGCTGGTTGA TGGAATTGAT CCCAACTTCA AAATGGAGCA CCAAAGTAAG 
CGTTCCCCAT TACATGCTGC TGCGGAGGCT GGCCACGTGG ACATCTGCCA CATGCTGGTT 
CAGGCGGGTG CCAATATTGA CACTTGCTCA GAGGACCAGC GGACCCCACT GATGGAGGCT 
GCAGAGAACA ACCACTTGGA TGCAGTGAAG TACCTCATCA AGGCTGGAGC ACAGGTGGAT 
CCGAAGGACG CAGAGGGCTC CACATGTTTG CATTTGGCTG CCAAGAAAGG CCACTATGAT 
GTGGTTCAGT ATCTGCTTTC AAATGGACAG ATGGATGTCA ACTGCCAGGA TGACGGTGGA 
TGGACACCTA TGATCTGGGC CACTGAGTAC AAGCACGTGG AGCTGGTGAA GCTGCTGCTG 
TCTAAGGGCT CTGACATCAA CATCCGGGAC AACGAGGAGA ACATTTGTCT GCACTGGGCA 
GCATTTTCAG GCTGTGTGGA CATAGCTGAA ATACTTCTGG CTGCCAAGTG TGACCTGCAT 
GCTGTGAATA TCCATGGAGA CTCACCCCTG CACATCGCAG CCAGGGAGAA TCGCTACGAC 
TGTGTTGTCC TCTTTCTTTC TCGGGATTCA GATGTTACTC TGAAAAACAA GGAAGGAGAG 
ACTCCCTTGC AGTGTGCAAG TCTCAGTTCG CAGGTGTGGA GTGCATTGCA GATGAGCAAA 
GCACTTCGGG ACTCAGCCCC TGACAAGCCC GTTGCTGTTG AGAAGACGGT GAGCAGGGAT 
ATCGCTCGAG GGTATGAGCG CATTCCCATT CCCTGTGTCA ATGCTGTGGA CAGTGAGCTG 
TGTCCTACCA ACTATAAGTA TGTCTCCCAG AACTGTGTGA CATCCCCCAT GAACATTGAC 
AGGAACATCA CTCATTTGCA GTACTGCGTG TGTGTAGATG ACTGCTCCTC TAGCACCTGC 
ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG TATGATAAGG ATGGCCGACT TCTGCCTGAG 
TTTAACATGG CAGAACCACC CTTGATCTTC GAGTGCAATC ATGCCTGCTC ATGCTGGAGG 
AACTGCCGCA ATCGTGTGGT GCAAAATGGT CTCAGGGCAA GGCTGCAGCT TTATCGGACA 
CAGGACATGG GCTGGGGTGT GCGGTCCCTC CAGGATATCC CACTGGGCAC CTTTGTCTGC 
GAATACGTAG GGGAGCTGAT TTCGGACTCT GAAGCTGATG TTCGGGAAGA GGACTCTTAC 
CTCTTTGATC TTGACAATAA GGATGGAGAG GTATACTGCA TTGACGCTCG GTTCTATGGG 
AATGTCAGCC GGTTCATAAA CCACCACTGC GAACCCAACC TTGTGCCTGT GCGAGTGTTC 
ATGTCACACC AGGACCTGCG GTTTCCCAGG ATTGCCTTCT TCAGTACCCG CCTGATTCAG 
GCTGGGGAGC AGCTCGGGTT CGACTACGGG GAGCGCTTTT GGGACGTCAA GGGCAAGCTC 
TTCAGTTGCC GGTGTGGGTC TTCCAAGTGT CGGCACTCAA GCGCAGCCCT GGCCCAGAGG 
CAAGCCAGTG CAGCCCAGGA GCCTCAGGAG AATGGCCTTC CAGATACCAG CTCTGCAGCC 
GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498426.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498489.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu29804
Clone ID Related Accession (Same CDS sequence)	XM_006498426.2
Accession Version	XM_006498426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA ATTGGGCCGG 
TGTACAAACA GCGTGGTCAA GTATGAGCTG ATGCGCCCAT CCAACAAAGC ACCGCTCTTG 
GTGTTATGTG AAGACCATCG GGGTCGCATG GTGAAGCACC AGTGCTGTCC TGGCTGCGGC 
TACTTCTGCA CGGCGGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498489.1
CDS	27..3941
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498426.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498426.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA 
ACCTATAGTT TAGTTCCCAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG 
ATGTTTAAAA GCATAACCCA TTCCACTGTG GGCGCCAAGG GCGAGAAAGC CTTAGATGAT 
AGTGCCCTGC ATGTAAATGG CGAGAGCTTG GAGATGGACT CAGAAGATGA AGACTCCGAT 
GAGCTGGAGG ATGACGAGGA CCATGGAGCT GAGCAAGCGG CTGCATTTCC CACCGAGGAT 
AGTAGGACTT CTAAAGAGAG CATGTCTGAG ACTGACCGGG CTGCAAAGAT GGATGGAGAT 
TCAGAGGAGG AACAGGAGTC TCCCGACACA GGGGAGGATG AAGATGGTGG AGACGAGTCT 
GACCTGAGTT CTGAATCCAG TATCAAGAAG AAATTTCTCA AGAGGAGAGG GAAGACTGAC 
AGCCCCTGGA TCAAACCTGC TCGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCGAGCAGC 
ATGCTTGGTT CCGAGGCTTG TAAGTCATCT CCAGGAAGCA TGGAGCAGGC AGCTCTGGGA 
GACAGCGCTG GCTATATGGA AGTTTCCCTG GATTCCCTGG ATCTCCGTGT CAGAGGAATT 
CTGTCTTCCC AGACAGAAAA TGAAGGGCTG GCCAGTGGTC CGGATGTGCT GGGGACGGAT 
GGCCTCCAGG AAGTGCCTCT CTGCAGCTGC CGAATGGAAA CCCCCAAGAG CCGCGAGATC 
AGCACCCTGG CCAACAACCA GTGCATGGCC ACTGAGAGCG TGGATCACGA ATTGGGCCGG 
TGTACAAACA GCGTGGTCAA GTATGAGCTG ATGCGCCCAT CCAACAAAGC ACCGCTCTTG 
GTGTTATGTG AAGACCATCG GGGTCGCATG GTGAAGCACC AGTGCTGTCC TGGCTGCGGC 
TACTTCTGCA CGGCGGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498427.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498490.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

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CloneID	OMu29805
Clone ID Related Accession (Same CDS sequence)	XM_006498427.2
Accession Version	XM_006498427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGGTAAAGA TACACCTATG GCTGCTGATG AAGGTTCCAC AGAGAAACAA 
GAAGGAGAGA CTCCCATGGC TGCAGATGGA GAAACAAATG GGTCTTGTGA AAAGAGTGGG 
GATCCCAGCC ATCTAAATGC ACCCAAACAC ACTCAGGAGA ACACAAGAGC TAGCCCACAG 
GAAGGCACCA ACAGAGTGTC TCGGGTGGCA GAAAATGGGG TTTCAGAAAG AGACACAGAA 
GTGGGGAAGC AAAACCATGT CACAGCTGAC GACTTCATGC AGACATCTGT CATTGGCAGC 
AATGGATATT TCTTAAATAA ACCAGCCCTG CAGGGGCAGC CGTTGAGGAC TCCCAACATT 
CTAACCTCCT CGCTTCCTGG TCATGCTGCA AAAACTCTTC CTGGAGGAGC CAGTAAATGC 
AGGACTCTGA GTGCACTTCC TCAGACACCA ACCACAGCAC CCACTGTGCC TGGGGAAGGG 
AGTGCAGACA CAGAGGACAG AAAGCCTACA GCCTCGGGCA CTGATGTCAG GGTTCACAGG 
GCACGCAAGA CCATGCCGAA GTCCATCTTG GGCCTGCATG CAGCCAGCAA AGACCATAGA 
GAAGTTCAAG ACCATAAGGA ACCAAAAGAG GATATCAACA GAAACATTTC TGAATGTGGA 
CGACAGCAGC TTTTACCAAC CTTCCCAGCC CTCCACCAGT CGCTACCTCA GAATCAGTGC 
TACATGGCCA CCACAAAGTC CCAGACAGCT TGCTTGCCTT TTGTTTTAGC AGCTGCAGTA 
TCTCGGAAGA AAAAACGAAG AATGGGAACC TATAGTTTAG TTCCCAAGAA AAAGACCAAA 
GTATTAAAAC AGAGGACGGT GATTGAGATG TTTAAAAGCA TAACCCATTC CACTGTGGGC 
GCCAAGGGCG AGAAAGCCTT AGATGATAGT GCCCTGCATG TAAATGGCGA GAGCTTGGAG 
ATGGACTCAG AAGATGAAGA CTCCGATGAG CTGGAGGATG ACGAGGACCA TGGAGCTGAG 
CAAGCGGCTG CATTTCCCAC CGAGGATAGT AGGACTTCTA AAGAGAGCAT GTCTGAGACT 
GACCGGGCTG CAAAGATGGA TGGAGATTCA GAGGAGGAAC AGGAGTCTCC CGACACAGGG 
GAGGATGAAG ATGGTGGAGA CGAGTCTGAC CTGAGTTCTG AATCCAGTAT CAAGAAGAAA 
TTTCTCAAGA GGAGAGGGAA GACTGACAGC CCCTGGATCA AACCTGCTCG GAAAAGGAGG 
CGGAGAAGTA GAAAGAAGCC GAGCAGCATG CTTGGTTCCG AGGCTTGTAA GTCATCTCCA 
GGAAGCATGG AGCAGGCAGC TCTGGGAGAC AGCGCTGGCT ATATGGAAGT TTCCCTGGAT 
TCCCTGGATC TCCGTGTCAG AGGAATTCTG TCTTCCCAGA CAGAAAATGA AGGGCTGGCC 
AGTGGTCCGG ATGTGCTGGG GACGGATGGC CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA 
ATGGAAACCC CCAAGAGCCG CGAGATCAGC ACCCTGGCCA ACAACCAGTG CATGGCCACT 
GAGAGCGTGG ATCACGAATT GGGCCGGTGT ACAAACAGCG TGGTCAAGTA TGAGCTGATG 
CGCCCATCCA ACAAAGCACC GCTCTTGGTG TTATGTGAAG ACCATCGGGG TCGCATGGTG 
AAGCACCAGT GCTGTCCTGG CTGCGGCTAC TTCTGCACGG CGGGTAACTT CATGGAATGC 
CAGCCTGAGA GCAGCATCTC TCATCGTTTC CATAAGGACT GTGCCTCTCG AGTCAACAAT 
GCCAGCTACT GTCCCCATTG TGGGGAAGAA GCTTCCAAGG CCAAAGAGGT GACCATAGCA 
AAAGCAGACA CAACTTCCAC AGTGACCCTA GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT 
GAAGGCAGGG CTGACACGAC CACGGGCAGC ATTGCTGGAG CCCCAGAGGA TGAAAGATCG 
CAGAGCACAG CCCCCCAGGC ACCAGAGTGC TTCGACCCTG CCGGACCGGC TGGGCTCGTG 
AGGCCGACAT CTGGCCTTTC CCAGGGCCCA GGAAAGGAAA CCTTGGAAAG TGCTCTAATC 
GCTCTAGACT CTGAAAAACC CAAGAAACTT CGCTTCCACC CAAAGCAGCT GTACTTCTCT 
GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT CTCATGCTGG TTGATGGAAT TGATCCCAAC 
TTCAAAATGG AGCACCAAAG TAAGCGTTCC CCATTACATG CTGCTGCGGA GGCTGGCCAC 
GTGGACATCT GCCACATGCT GGTTCAGGCG GGTGCCAATA TTGACACTTG CTCAGAGGAC 
CAGCGGACCC CACTGATGGA GGCTGCAGAG AACAACCACT TGGATGCAGT GAAGTACCTC 
ATCAAGGCTG GAGCACAGGT GGATCCGAAG GACGCAGAGG GCTCCACATG TTTGCATTTG 
GCTGCCAAGA AAGGCCACTA TGATGTGGTT CAGTATCTGC TTTCAAATGG ACAGATGGAT 
GTCAACTGCC AGGATGACGG TGGATGGACA CCTATGATCT GGGCCACTGA GTACAAGCAC 
GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG GGCTCTGACA TCAACATCCG GGACAACGAG 
GAGAACATTT GTCTGCACTG GGCAGCATTT TCAGGCTGTG TGGACATAGC TGAAATACTT 
CTGGCTGCCA AGTGTGACCT GCATGCTGTG AATATCCATG GAGACTCACC CCTGCACATC 
GCAGCCAGGG AGAATCGCTA CGACTGTGTT GTCCTCTTTC TTTCTCGGGA TTCAGATGTT 
ACTCTGAAAA ACAAGGAAGG AGAGACTCCC TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG 
TGGAGTGCAT TGCAGATGAG CAAAGCACTT CGGGACTCAG CCCCTGACAA GCCCGTTGCT 
GTTGAGAAGA CGGTGAGCAG GGATATCGCT CGAGGGTATG AGCGCATTCC CATTCCCTGT 
GTCAATGCTG TGGACAGTGA GCTGTGTCCT ACCAACTATA AGTATGTCTC CCAGAACTGT 
GTGACATCCC CCATGAACAT TGACAGGAAC ATCACTCATT TGCAGTACTG CGTGTGTGTA 
GATGACTGCT CCTCTAGCAC CTGCATGTGT GGCCAGCTGA GCATGCGCTG CTGGTATGAT 
AAGGATGGCC GACTTCTGCC TGAGTTTAAC ATGGCAGAAC CACCCTTGAT CTTCGAGTGC 
AATCATGCCT GCTCATGCTG GAGGAACTGC CGCAATCGTG TGGTGCAAAA TGGTCTCAGG 
GCAAGGCTGC AGCTTTATCG GACACAGGAC ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT 
ATCCCACTGG GCACCTTTGT CTGCGAATAC GTAGGGGAGC TGATTTCGGA CTCTGAAGCT 
GATGTTCGGG AAGAGGACTC TTACCTCTTT GATCTTGACA ATAAGGATGG AGAGGTATAC 
TGCATTGACG CTCGGTTCTA TGGGAATGTC AGCCGGTTCA TAAACCACCA CTGCGAACCC 
AACCTTGTGC CTGTGCGAGT GTTCATGTCA CACCAGGACC TGCGGTTTCC CAGGATTGCC 
TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG GAGCAGCTCG GGTTCGACTA CGGGGAGCGC 
TTTTGGGACG TCAAGGGCAA GCTCTTCAGT TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC 
TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC AGTGCAGCCC AGGAGCCTCA GGAGAATGGC 
CTTCCAGATA CCAGCTCTGC AGCCGCTGCT GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498490.1
CDS	16..3897
Translation	MKQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498427.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498427.2

ORF Insert Sequence:

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGGTAAAGA TACACCTATG GCTGCTGATG AAGGTTCCAC AGAGAAACAA 
GAAGGAGAGA CTCCCATGGC TGCAGATGGA GAAACAAATG GGTCTTGTGA AAAGAGTGGG 
GATCCCAGCC ATCTAAATGC ACCCAAACAC ACTCAGGAGA ACACAAGAGC TAGCCCACAG 
GAAGGCACCA ACAGAGTGTC TCGGGTGGCA GAAAATGGGG TTTCAGAAAG AGACACAGAA 
GTGGGGAAGC AAAACCATGT CACAGCTGAC GACTTCATGC AGACATCTGT CATTGGCAGC 
AATGGATATT TCTTAAATAA ACCAGCCCTG CAGGGGCAGC CGTTGAGGAC TCCCAACATT 
CTAACCTCCT CGCTTCCTGG TCATGCTGCA AAAACTCTTC CTGGAGGAGC CAGTAAATGC 
AGGACTCTGA GTGCACTTCC TCAGACACCA ACCACAGCAC CCACTGTGCC TGGGGAAGGG 
AGTGCAGACA CAGAGGACAG AAAGCCTACA GCCTCGGGCA CTGATGTCAG GGTTCACAGG 
GCACGCAAGA CCATGCCGAA GTCCATCTTG GGCCTGCATG CAGCCAGCAA AGACCATAGA 
GAAGTTCAAG ACCATAAGGA ACCAAAAGAG GATATCAACA GAAACATTTC TGAATGTGGA 
CGACAGCAGC TTTTACCAAC CTTCCCAGCC CTCCACCAGT CGCTACCTCA GAATCAGTGC 
TACATGGCCA CCACAAAGTC CCAGACAGCT TGCTTGCCTT TTGTTTTAGC AGCTGCAGTA 
TCTCGGAAGA AAAAACGAAG AATGGGAACC TATAGTTTAG TTCCCAAGAA AAAGACCAAA 
GTATTAAAAC AGAGGACGGT GATTGAGATG TTTAAAAGCA TAACCCATTC CACTGTGGGC 
GCCAAGGGCG AGAAAGCCTT AGATGATAGT GCCCTGCATG TAAATGGCGA GAGCTTGGAG 
ATGGACTCAG AAGATGAAGA CTCCGATGAG CTGGAGGATG ACGAGGACCA TGGAGCTGAG 
CAAGCGGCTG CATTTCCCAC CGAGGATAGT AGGACTTCTA AAGAGAGCAT GTCTGAGACT 
GACCGGGCTG CAAAGATGGA TGGAGATTCA GAGGAGGAAC AGGAGTCTCC CGACACAGGG 
GAGGATGAAG ATGGTGGAGA CGAGTCTGAC CTGAGTTCTG AATCCAGTAT CAAGAAGAAA 
TTTCTCAAGA GGAGAGGGAA GACTGACAGC CCCTGGATCA AACCTGCTCG GAAAAGGAGG 
CGGAGAAGTA GAAAGAAGCC GAGCAGCATG CTTGGTTCCG AGGCTTGTAA GTCATCTCCA 
GGAAGCATGG AGCAGGCAGC TCTGGGAGAC AGCGCTGGCT ATATGGAAGT TTCCCTGGAT 
TCCCTGGATC TCCGTGTCAG AGGAATTCTG TCTTCCCAGA CAGAAAATGA AGGGCTGGCC 
AGTGGTCCGG ATGTGCTGGG GACGGATGGC CTCCAGGAAG TGCCTCTCTG CAGCTGCCGA 
ATGGAAACCC CCAAGAGCCG CGAGATCAGC ACCCTGGCCA ACAACCAGTG CATGGCCACT 
GAGAGCGTGG ATCACGAATT GGGCCGGTGT ACAAACAGCG TGGTCAAGTA TGAGCTGATG 
CGCCCATCCA ACAAAGCACC GCTCTTGGTG TTATGTGAAG ACCATCGGGG TCGCATGGTG 
AAGCACCAGT GCTGTCCTGG CTGCGGCTAC TTCTGCACGG CGGGTAACTT CATGGAATGC 
CAGCCTGAGA GCAGCATCTC TCATCGTTTC CATAAGGACT GTGCCTCTCG AGTCAACAAT 
GCCAGCTACT GTCCCCATTG TGGGGAAGAA GCTTCCAAGG CCAAAGAGGT GACCATAGCA 
AAAGCAGACA CAACTTCCAC AGTGACCCTA GCCCCTGGAC AGGAGAAGAG CCTGGCTGCT 
GAAGGCAGGG CTGACACGAC CACGGGCAGC ATTGCTGGAG CCCCAGAGGA TGAAAGATCG 
CAGAGCACAG CCCCCCAGGC ACCAGAGTGC TTCGACCCTG CCGGACCGGC TGGGCTCGTG 
AGGCCGACAT CTGGCCTTTC CCAGGGCCCA GGAAAGGAAA CCTTGGAAAG TGCTCTAATC 
GCTCTAGACT CTGAAAAACC CAAGAAACTT CGCTTCCACC CAAAGCAGCT GTACTTCTCT 
GCCAGGCAGG GTGAGCTGCA GAAGGTGCTT CTCATGCTGG TTGATGGAAT TGATCCCAAC 
TTCAAAATGG AGCACCAAAG TAAGCGTTCC CCATTACATG CTGCTGCGGA GGCTGGCCAC 
GTGGACATCT GCCACATGCT GGTTCAGGCG GGTGCCAATA TTGACACTTG CTCAGAGGAC 
CAGCGGACCC CACTGATGGA GGCTGCAGAG AACAACCACT TGGATGCAGT GAAGTACCTC 
ATCAAGGCTG GAGCACAGGT GGATCCGAAG GACGCAGAGG GCTCCACATG TTTGCATTTG 
GCTGCCAAGA AAGGCCACTA TGATGTGGTT CAGTATCTGC TTTCAAATGG ACAGATGGAT 
GTCAACTGCC AGGATGACGG TGGATGGACA CCTATGATCT GGGCCACTGA GTACAAGCAC 
GTGGAGCTGG TGAAGCTGCT GCTGTCTAAG GGCTCTGACA TCAACATCCG GGACAACGAG 
GAGAACATTT GTCTGCACTG GGCAGCATTT TCAGGCTGTG TGGACATAGC TGAAATACTT 
CTGGCTGCCA AGTGTGACCT GCATGCTGTG AATATCCATG GAGACTCACC CCTGCACATC 
GCAGCCAGGG AGAATCGCTA CGACTGTGTT GTCCTCTTTC TTTCTCGGGA TTCAGATGTT 
ACTCTGAAAA ACAAGGAAGG AGAGACTCCC TTGCAGTGTG CAAGTCTCAG TTCGCAGGTG 
TGGAGTGCAT TGCAGATGAG CAAAGCACTT CGGGACTCAG CCCCTGACAA GCCCGTTGCT 
GTTGAGAAGA CGGTGAGCAG GGATATCGCT CGAGGGTATG AGCGCATTCC CATTCCCTGT 
GTCAATGCTG TGGACAGTGA GCTGTGTCCT ACCAACTATA AGTATGTCTC CCAGAACTGT 
GTGACATCCC CCATGAACAT TGACAGGAAC ATCACTCATT TGCAGTACTG CGTGTGTGTA 
GATGACTGCT CCTCTAGCAC CTGCATGTGT GGCCAGCTGA GCATGCGCTG CTGGTATGAT 
AAGGATGGCC GACTTCTGCC TGAGTTTAAC ATGGCAGAAC CACCCTTGAT CTTCGAGTGC 
AATCATGCCT GCTCATGCTG GAGGAACTGC CGCAATCGTG TGGTGCAAAA TGGTCTCAGG 
GCAAGGCTGC AGCTTTATCG GACACAGGAC ATGGGCTGGG GTGTGCGGTC CCTCCAGGAT 
ATCCCACTGG GCACCTTTGT CTGCGAATAC GTAGGGGAGC TGATTTCGGA CTCTGAAGCT 
GATGTTCGGG AAGAGGACTC TTACCTCTTT GATCTTGACA ATAAGGATGG AGAGGTATAC 
TGCATTGACG CTCGGTTCTA TGGGAATGTC AGCCGGTTCA TAAACCACCA CTGCGAACCC 
AACCTTGTGC CTGTGCGAGT GTTCATGTCA CACCAGGACC TGCGGTTTCC CAGGATTGCC 
TTCTTCAGTA CCCGCCTGAT TCAGGCTGGG GAGCAGCTCG GGTTCGACTA CGGGGAGCGC 
TTTTGGGACG TCAAGGGCAA GCTCTTCAGT TGCCGGTGTG GGTCTTCCAA GTGTCGGCAC 
TCAAGCGCAG CCCTGGCCCA GAGGCAAGCC AGTGCAGCCC AGGAGCCTCA GGAGAATGGC 
CTTCCAGATA CCAGCTCTGC AGCCGCTGCT GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498429.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498492.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu29807
Clone ID Related Accession (Same CDS sequence)	XM_006498429.2
Accession Version	XM_006498429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTTGCTTG CCTTTTGTTT TAGCAGCTGC AGTATCTCGG 
AAGAAAAAAC GAAGAATGGG AACCTATAGT TTAGTTCCCA AGAAAAAGAC CAAAGTATTA 
AAACAGAGGA CGGTGATTGA GATGTTTAAA AGCATAACCC ATTCCACTGT GGGCGCCAAG 
GGCGAGAAAG CCTTAGATGA TAGTGCCCTG CATGTAAATG GCGAGAGCTT GGAGATGGAC 
TCAGAAGATG AAGACTCCGA TGAGCTGGAG GATGACGAGG ACCATGGAGC TGAGCAAGCG 
GCTGCATTTC CCACCGAGGA TAGTAGGACT TCTAAAGAGA GCATGTCTGA GACTGACCGG 
GCTGCAAAGA TGGATGGAGA TTCAGAGGAG GAACAGGAGT CTCCCGACAC AGGGGAGGAT 
GAAGATGGTG GAGACGAGTC TGACCTGAGT TCTGAATCCA GTATCAAGAA GAAATTTCTC 
AAGAGGAGAG GGAAGACTGA CAGCCCCTGG ATCAAACCTG CTCGGAAAAG GAGGCGGAGA 
AGTAGAAAGA AGCCGAGCAG CATGCTTGGT TCCGAGGCTT GTAAGTCATC TCCAGGAAGC 
ATGGAGCAGG CAGCTCTGGG AGACAGCGCT GGCTATATGG AAGTTTCCCT GGATTCCCTG 
GATCTCCGTG TCAGAGGAAT TCTGTCTTCC CAGACAGAAA ATGAAGGGCT GGCCAGTGGT 
CCGGATGTGC TGGGGACGGA TGGCCTCCAG GAAGTGCCTC TCTGCAGCTG CCGAATGGAA 
ACCCCCAAGA GCCGCGAGAT CAGCACCCTG GCCAACAACC AGTGCATGGC CACTGAGAGC 
GTGGATCACG AATTGGGCCG GTGTACAAAC AGCGTGGTCA AGTATGAGCT GATGCGCCCA 
TCCAACAAAG CACCGCTCTT GGTGTTATGT GAAGACCATC GGGGTCGCAT GGTGAAGCAC 
CAGTGCTGTC CTGGCTGCGG CTACTTCTGC ACGGCGGGTA ACTTCATGGA ATGCCAGCCT 
GAGAGCAGCA TCTCTCATCG TTTCCATAAG GACTGTGCCT CTCGAGTCAA CAATGCCAGC 
TACTGTCCCC ATTGTGGGGA AGAAGCTTCC AAGGCCAAAG AGGTGACCAT AGCAAAAGCA 
GACACAACTT CCACAGTGAC CCTAGCCCCT GGACAGGAGA AGAGCCTGGC TGCTGAAGGC 
AGGGCTGACA CGACCACGGG CAGCATTGCT GGAGCCCCAG AGGATGAAAG ATCGCAGAGC 
ACAGCCCCCC AGGCACCAGA GTGCTTCGAC CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG 
ACATCTGGCC TTTCCCAGGG CCCAGGAAAG GAAACCTTGG AAAGTGCTCT AATCGCTCTA 
GACTCTGAAA AACCCAAGAA ACTTCGCTTC CACCCAAAGC AGCTGTACTT CTCTGCCAGG 
CAGGGTGAGC TGCAGAAGGT GCTTCTCATG CTGGTTGATG GAATTGATCC CAACTTCAAA 
ATGGAGCACC AAAGTAAGCG TTCCCCATTA CATGCTGCTG CGGAGGCTGG CCACGTGGAC 
ATCTGCCACA TGCTGGTTCA GGCGGGTGCC AATATTGACA CTTGCTCAGA GGACCAGCGG 
ACCCCACTGA TGGAGGCTGC AGAGAACAAC CACTTGGATG CAGTGAAGTA CCTCATCAAG 
GCTGGAGCAC AGGTGGATCC GAAGGACGCA GAGGGCTCCA CATGTTTGCA TTTGGCTGCC 
AAGAAAGGCC ACTATGATGT GGTTCAGTAT CTGCTTTCAA ATGGACAGAT GGATGTCAAC 
TGCCAGGATG ACGGTGGATG GACACCTATG ATCTGGGCCA CTGAGTACAA GCACGTGGAG 
CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT GACATCAACA TCCGGGACAA CGAGGAGAAC 
ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC TGTGTGGACA TAGCTGAAAT ACTTCTGGCT 
GCCAAGTGTG ACCTGCATGC TGTGAATATC CATGGAGACT CACCCCTGCA CATCGCAGCC 
AGGGAGAATC GCTACGACTG TGTTGTCCTC TTTCTTTCTC GGGATTCAGA TGTTACTCTG 
AAAAACAAGG AAGGAGAGAC TCCCTTGCAG TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT 
GCATTGCAGA TGAGCAAAGC ACTTCGGGAC TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG 
AAGACGGTGA GCAGGGATAT CGCTCGAGGG TATGAGCGCA TTCCCATTCC CTGTGTCAAT 
GCTGTGGACA GTGAGCTGTG TCCTACCAAC TATAAGTATG TCTCCCAGAA CTGTGTGACA 
TCCCCCATGA ACATTGACAG GAACATCACT CATTTGCAGT ACTGCGTGTG TGTAGATGAC 
TGCTCCTCTA GCACCTGCAT GTGTGGCCAG CTGAGCATGC GCTGCTGGTA TGATAAGGAT 
GGCCGACTTC TGCCTGAGTT TAACATGGCA GAACCACCCT TGATCTTCGA GTGCAATCAT 
GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT CGTGTGGTGC AAAATGGTCT CAGGGCAAGG 
CTGCAGCTTT ATCGGACACA GGACATGGGC TGGGGTGTGC GGTCCCTCCA GGATATCCCA 
CTGGGCACCT TTGTCTGCGA ATACGTAGGG GAGCTGATTT CGGACTCTGA AGCTGATGTT 
CGGGAAGAGG ACTCTTACCT CTTTGATCTT GACAATAAGG ATGGAGAGGT ATACTGCATT 
GACGCTCGGT TCTATGGGAA TGTCAGCCGG TTCATAAACC ACCACTGCGA ACCCAACCTT 
GTGCCTGTGC GAGTGTTCAT GTCACACCAG GACCTGCGGT TTCCCAGGAT TGCCTTCTTC 
AGTACCCGCC TGATTCAGGC TGGGGAGCAG CTCGGGTTCG ACTACGGGGA GCGCTTTTGG 
GACGTCAAGG GCAAGCTCTT CAGTTGCCGG TGTGGGTCTT CCAAGTGTCG GCACTCAAGC 
GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA 
GATACCAGCT CTGCAGCCGC TGCTGACCCC CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498492.1
CDS	16..3891
Translation	MKQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498429.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498429.2

ORF Insert Sequence:

ATGAAGCAGG CAGTTCTGGC CAAGCAAGAG ACCAAGCAGG ATTGCTGCAT GAAAACTGAG 
CTGCTAAGGG AAGATACACC TATGGCTGCT GATGAAGGTT CCACAGAGAA ACAAGAAGGA 
GAGACTCCCA TGGCTGCAGA TGGAGAAACA AATGGGTCTT GTGAAAAGAG TGGGGATCCC 
AGCCATCTAA ATGCACCCAA ACACACTCAG GAGAACACAA GAGCTAGCCC ACAGGAAGGC 
ACCAACAGAG TGTCTCGGGT GGCAGAAAAT GGGGTTTCAG AAAGAGACAC AGAAGTGGGG 
AAGCAAAACC ATGTCACAGC TGACGACTTC ATGCAGACAT CTGTCATTGG CAGCAATGGA 
TATTTCTTAA ATAAACCAGC CCTGCAGGGG CAGCCGTTGA GGACTCCCAA CATTCTAACC 
TCCTCGCTTC CTGGTCATGC TGCAAAAACT CTTCCTGGAG GAGCCAGTAA ATGCAGGACT 
CTGAGTGCAC TTCCTCAGAC ACCAACCACA GCACCCACTG TGCCTGGGGA AGGGAGTGCA 
GACACAGAGG ACAGAAAGCC TACAGCCTCG GGCACTGATG TCAGGGTTCA CAGGGCACGC 
AAGACCATGC CGAAGTCCAT CTTGGGCCTG CATGCAGCCA GCAAAGACCA TAGAGAAGTT 
CAAGACCATA AGGAACCAAA AGAGGATATC AACAGAAACA TTTCTGAATG TGGACGACAG 
CAGCTTTTAC CAACCTTCCC AGCCCTCCAC CAGTCGCTAC CTCAGAATCA GTGCTACATG 
GCCACCACAA AGTCCCAGAC AGCTTGCTTG CCTTTTGTTT TAGCAGCTGC AGTATCTCGG 
AAGAAAAAAC GAAGAATGGG AACCTATAGT TTAGTTCCCA AGAAAAAGAC CAAAGTATTA 
AAACAGAGGA CGGTGATTGA GATGTTTAAA AGCATAACCC ATTCCACTGT GGGCGCCAAG 
GGCGAGAAAG CCTTAGATGA TAGTGCCCTG CATGTAAATG GCGAGAGCTT GGAGATGGAC 
TCAGAAGATG AAGACTCCGA TGAGCTGGAG GATGACGAGG ACCATGGAGC TGAGCAAGCG 
GCTGCATTTC CCACCGAGGA TAGTAGGACT TCTAAAGAGA GCATGTCTGA GACTGACCGG 
GCTGCAAAGA TGGATGGAGA TTCAGAGGAG GAACAGGAGT CTCCCGACAC AGGGGAGGAT 
GAAGATGGTG GAGACGAGTC TGACCTGAGT TCTGAATCCA GTATCAAGAA GAAATTTCTC 
AAGAGGAGAG GGAAGACTGA CAGCCCCTGG ATCAAACCTG CTCGGAAAAG GAGGCGGAGA 
AGTAGAAAGA AGCCGAGCAG CATGCTTGGT TCCGAGGCTT GTAAGTCATC TCCAGGAAGC 
ATGGAGCAGG CAGCTCTGGG AGACAGCGCT GGCTATATGG AAGTTTCCCT GGATTCCCTG 
GATCTCCGTG TCAGAGGAAT TCTGTCTTCC CAGACAGAAA ATGAAGGGCT GGCCAGTGGT 
CCGGATGTGC TGGGGACGGA TGGCCTCCAG GAAGTGCCTC TCTGCAGCTG CCGAATGGAA 
ACCCCCAAGA GCCGCGAGAT CAGCACCCTG GCCAACAACC AGTGCATGGC CACTGAGAGC 
GTGGATCACG AATTGGGCCG GTGTACAAAC AGCGTGGTCA AGTATGAGCT GATGCGCCCA 
TCCAACAAAG CACCGCTCTT GGTGTTATGT GAAGACCATC GGGGTCGCAT GGTGAAGCAC 
CAGTGCTGTC CTGGCTGCGG CTACTTCTGC ACGGCGGGTA ACTTCATGGA ATGCCAGCCT 
GAGAGCAGCA TCTCTCATCG TTTCCATAAG GACTGTGCCT CTCGAGTCAA CAATGCCAGC 
TACTGTCCCC ATTGTGGGGA AGAAGCTTCC AAGGCCAAAG AGGTGACCAT AGCAAAAGCA 
GACACAACTT CCACAGTGAC CCTAGCCCCT GGACAGGAGA AGAGCCTGGC TGCTGAAGGC 
AGGGCTGACA CGACCACGGG CAGCATTGCT GGAGCCCCAG AGGATGAAAG ATCGCAGAGC 
ACAGCCCCCC AGGCACCAGA GTGCTTCGAC CCTGCCGGAC CGGCTGGGCT CGTGAGGCCG 
ACATCTGGCC TTTCCCAGGG CCCAGGAAAG GAAACCTTGG AAAGTGCTCT AATCGCTCTA 
GACTCTGAAA AACCCAAGAA ACTTCGCTTC CACCCAAAGC AGCTGTACTT CTCTGCCAGG 
CAGGGTGAGC TGCAGAAGGT GCTTCTCATG CTGGTTGATG GAATTGATCC CAACTTCAAA 
ATGGAGCACC AAAGTAAGCG TTCCCCATTA CATGCTGCTG CGGAGGCTGG CCACGTGGAC 
ATCTGCCACA TGCTGGTTCA GGCGGGTGCC AATATTGACA CTTGCTCAGA GGACCAGCGG 
ACCCCACTGA TGGAGGCTGC AGAGAACAAC CACTTGGATG CAGTGAAGTA CCTCATCAAG 
GCTGGAGCAC AGGTGGATCC GAAGGACGCA GAGGGCTCCA CATGTTTGCA TTTGGCTGCC 
AAGAAAGGCC ACTATGATGT GGTTCAGTAT CTGCTTTCAA ATGGACAGAT GGATGTCAAC 
TGCCAGGATG ACGGTGGATG GACACCTATG ATCTGGGCCA CTGAGTACAA GCACGTGGAG 
CTGGTGAAGC TGCTGCTGTC TAAGGGCTCT GACATCAACA TCCGGGACAA CGAGGAGAAC 
ATTTGTCTGC ACTGGGCAGC ATTTTCAGGC TGTGTGGACA TAGCTGAAAT ACTTCTGGCT 
GCCAAGTGTG ACCTGCATGC TGTGAATATC CATGGAGACT CACCCCTGCA CATCGCAGCC 
AGGGAGAATC GCTACGACTG TGTTGTCCTC TTTCTTTCTC GGGATTCAGA TGTTACTCTG 
AAAAACAAGG AAGGAGAGAC TCCCTTGCAG TGTGCAAGTC TCAGTTCGCA GGTGTGGAGT 
GCATTGCAGA TGAGCAAAGC ACTTCGGGAC TCAGCCCCTG ACAAGCCCGT TGCTGTTGAG 
AAGACGGTGA GCAGGGATAT CGCTCGAGGG TATGAGCGCA TTCCCATTCC CTGTGTCAAT 
GCTGTGGACA GTGAGCTGTG TCCTACCAAC TATAAGTATG TCTCCCAGAA CTGTGTGACA 
TCCCCCATGA ACATTGACAG GAACATCACT CATTTGCAGT ACTGCGTGTG TGTAGATGAC 
TGCTCCTCTA GCACCTGCAT GTGTGGCCAG CTGAGCATGC GCTGCTGGTA TGATAAGGAT 
GGCCGACTTC TGCCTGAGTT TAACATGGCA GAACCACCCT TGATCTTCGA GTGCAATCAT 
GCCTGCTCAT GCTGGAGGAA CTGCCGCAAT CGTGTGGTGC AAAATGGTCT CAGGGCAAGG 
CTGCAGCTTT ATCGGACACA GGACATGGGC TGGGGTGTGC GGTCCCTCCA GGATATCCCA 
CTGGGCACCT TTGTCTGCGA ATACGTAGGG GAGCTGATTT CGGACTCTGA AGCTGATGTT 
CGGGAAGAGG ACTCTTACCT CTTTGATCTT GACAATAAGG ATGGAGAGGT ATACTGCATT 
GACGCTCGGT TCTATGGGAA TGTCAGCCGG TTCATAAACC ACCACTGCGA ACCCAACCTT 
GTGCCTGTGC GAGTGTTCAT GTCACACCAG GACCTGCGGT TTCCCAGGAT TGCCTTCTTC 
AGTACCCGCC TGATTCAGGC TGGGGAGCAG CTCGGGTTCG ACTACGGGGA GCGCTTTTGG 
GACGTCAAGG GCAAGCTCTT CAGTTGCCGG TGTGGGTCTT CCAAGTGTCG GCACTCAAGC 
GCAGCCCTGG CCCAGAGGCA AGCCAGTGCA GCCCAGGAGC CTCAGGAGAA TGGCCTTCCA 
GATACCAGCT CTGCAGCCGC TGCTGACCCC CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498430.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498493.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu29808
Clone ID Related Accession (Same CDS sequence)	XM_006498430.2
Accession Version	XM_006498430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAATTG 
GGCCGGTGTA CAAACAGCGT GGTCAAGTAT GAGCTGATGC GCCCATCCAA CAAAGCACCG 
CTCTTGGTGT TATGTGAAGA CCATCGGGGT CGCATGGTGA AGCACCAGTG CTGTCCTGGC 
TGCGGCTACT TCTGCACGGC GGGTAACTTC ATGGAATGCC AGCCTGAGAG CAGCATCTCT 
CATCGTTTCC ATAAGGACTG TGCCTCTCGA GTCAACAATG CCAGCTACTG TCCCCATTGT 
GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG ACCATAGCAA AAGCAGACAC AACTTCCACA 
GTGACCCTAG CCCCTGGACA GGAGAAGAGC CTGGCTGCTG AAGGCAGGGC TGACACGACC 
ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT GAAAGATCGC AGAGCACAGC CCCCCAGGCA 
CCAGAGTGCT TCGACCCTGC CGGACCGGCT GGGCTCGTGA GGCCGACATC TGGCCTTTCC 
CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT GCTCTAATCG CTCTAGACTC TGAAAAACCC 
AAGAAACTTC GCTTCCACCC AAAGCAGCTG TACTTCTCTG CCAGGCAGGG TGAGCTGCAG 
AAGGTGCTTC TCATGCTGGT TGATGGAATT GATCCCAACT TCAAAATGGA GCACCAAAGT 
AAGCGTTCCC CATTACATGC TGCTGCGGAG GCTGGCCACG TGGACATCTG CCACATGCTG 
GTTCAGGCGG GTGCCAATAT TGACACTTGC TCAGAGGACC AGCGGACCCC ACTGATGGAG 
GCTGCAGAGA ACAACCACTT GGATGCAGTG AAGTACCTCA TCAAGGCTGG AGCACAGGTG 
GATCCGAAGG ACGCAGAGGG CTCCACATGT TTGCATTTGG CTGCCAAGAA AGGCCACTAT 
GATGTGGTTC AGTATCTGCT TTCAAATGGA CAGATGGATG TCAACTGCCA GGATGACGGT 
GGATGGACAC CTATGATCTG GGCCACTGAG TACAAGCACG TGGAGCTGGT GAAGCTGCTG 
CTGTCTAAGG GCTCTGACAT CAACATCCGG GACAACGAGG AGAACATTTG TCTGCACTGG 
GCAGCATTTT CAGGCTGTGT GGACATAGCT GAAATACTTC TGGCTGCCAA GTGTGACCTG 
CATGCTGTGA ATATCCATGG AGACTCACCC CTGCACATCG CAGCCAGGGA GAATCGCTAC 
GACTGTGTTG TCCTCTTTCT TTCTCGGGAT TCAGATGTTA CTCTGAAAAA CAAGGAAGGA 
GAGACTCCCT TGCAGTGTGC AAGTCTCAGT TCGCAGGTGT GGAGTGCATT GCAGATGAGC 
AAAGCACTTC GGGACTCAGC CCCTGACAAG CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG 
GATATCGCTC GAGGGTATGA GCGCATTCCC ATTCCCTGTG TCAATGCTGT GGACAGTGAG 
CTGTGTCCTA CCAACTATAA GTATGTCTCC CAGAACTGTG TGACATCCCC CATGAACATT 
GACAGGAACA TCACTCATTT GCAGTACTGC GTGTGTGTAG ATGACTGCTC CTCTAGCACC 
TGCATGTGTG GCCAGCTGAG CATGCGCTGC TGGTATGATA AGGATGGCCG ACTTCTGCCT 
GAGTTTAACA TGGCAGAACC ACCCTTGATC TTCGAGTGCA ATCATGCCTG CTCATGCTGG 
AGGAACTGCC GCAATCGTGT GGTGCAAAAT GGTCTCAGGG CAAGGCTGCA GCTTTATCGG 
ACACAGGACA TGGGCTGGGG TGTGCGGTCC CTCCAGGATA TCCCACTGGG CACCTTTGTC 
TGCGAATACG TAGGGGAGCT GATTTCGGAC TCTGAAGCTG ATGTTCGGGA AGAGGACTCT 
TACCTCTTTG ATCTTGACAA TAAGGATGGA GAGGTATACT GCATTGACGC TCGGTTCTAT 
GGGAATGTCA GCCGGTTCAT AAACCACCAC TGCGAACCCA ACCTTGTGCC TGTGCGAGTG 
TTCATGTCAC ACCAGGACCT GCGGTTTCCC AGGATTGCCT TCTTCAGTAC CCGCCTGATT 
CAGGCTGGGG AGCAGCTCGG GTTCGACTAC GGGGAGCGCT TTTGGGACGT CAAGGGCAAG 
CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG TGTCGGCACT CAAGCGCAGC CCTGGCCCAG 
AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG GAGAATGGCC TTCCAGATAC CAGCTCTGCA 
GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498493.1
CDS	27..3827
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498430.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498430.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGGCTGGCCA GTGGTCCGGA TGTGCTGGGG 
ACGGATGGCC TCCAGGAAGT GCCTCTCTGC AGCTGCCGAA TGGAAACCCC CAAGAGCCGC 
GAGATCAGCA CCCTGGCCAA CAACCAGTGC ATGGCCACTG AGAGCGTGGA TCACGAATTG 
GGCCGGTGTA CAAACAGCGT GGTCAAGTAT GAGCTGATGC GCCCATCCAA CAAAGCACCG 
CTCTTGGTGT TATGTGAAGA CCATCGGGGT CGCATGGTGA AGCACCAGTG CTGTCCTGGC 
TGCGGCTACT TCTGCACGGC GGGTAACTTC ATGGAATGCC AGCCTGAGAG CAGCATCTCT 
CATCGTTTCC ATAAGGACTG TGCCTCTCGA GTCAACAATG CCAGCTACTG TCCCCATTGT 
GGGGAAGAAG CTTCCAAGGC CAAAGAGGTG ACCATAGCAA AAGCAGACAC AACTTCCACA 
GTGACCCTAG CCCCTGGACA GGAGAAGAGC CTGGCTGCTG AAGGCAGGGC TGACACGACC 
ACGGGCAGCA TTGCTGGAGC CCCAGAGGAT GAAAGATCGC AGAGCACAGC CCCCCAGGCA 
CCAGAGTGCT TCGACCCTGC CGGACCGGCT GGGCTCGTGA GGCCGACATC TGGCCTTTCC 
CAGGGCCCAG GAAAGGAAAC CTTGGAAAGT GCTCTAATCG CTCTAGACTC TGAAAAACCC 
AAGAAACTTC GCTTCCACCC AAAGCAGCTG TACTTCTCTG CCAGGCAGGG TGAGCTGCAG 
AAGGTGCTTC TCATGCTGGT TGATGGAATT GATCCCAACT TCAAAATGGA GCACCAAAGT 
AAGCGTTCCC CATTACATGC TGCTGCGGAG GCTGGCCACG TGGACATCTG CCACATGCTG 
GTTCAGGCGG GTGCCAATAT TGACACTTGC TCAGAGGACC AGCGGACCCC ACTGATGGAG 
GCTGCAGAGA ACAACCACTT GGATGCAGTG AAGTACCTCA TCAAGGCTGG AGCACAGGTG 
GATCCGAAGG ACGCAGAGGG CTCCACATGT TTGCATTTGG CTGCCAAGAA AGGCCACTAT 
GATGTGGTTC AGTATCTGCT TTCAAATGGA CAGATGGATG TCAACTGCCA GGATGACGGT 
GGATGGACAC CTATGATCTG GGCCACTGAG TACAAGCACG TGGAGCTGGT GAAGCTGCTG 
CTGTCTAAGG GCTCTGACAT CAACATCCGG GACAACGAGG AGAACATTTG TCTGCACTGG 
GCAGCATTTT CAGGCTGTGT GGACATAGCT GAAATACTTC TGGCTGCCAA GTGTGACCTG 
CATGCTGTGA ATATCCATGG AGACTCACCC CTGCACATCG CAGCCAGGGA GAATCGCTAC 
GACTGTGTTG TCCTCTTTCT TTCTCGGGAT TCAGATGTTA CTCTGAAAAA CAAGGAAGGA 
GAGACTCCCT TGCAGTGTGC AAGTCTCAGT TCGCAGGTGT GGAGTGCATT GCAGATGAGC 
AAAGCACTTC GGGACTCAGC CCCTGACAAG CCCGTTGCTG TTGAGAAGAC GGTGAGCAGG 
GATATCGCTC GAGGGTATGA GCGCATTCCC ATTCCCTGTG TCAATGCTGT GGACAGTGAG 
CTGTGTCCTA CCAACTATAA GTATGTCTCC CAGAACTGTG TGACATCCCC CATGAACATT 
GACAGGAACA TCACTCATTT GCAGTACTGC GTGTGTGTAG ATGACTGCTC CTCTAGCACC 
TGCATGTGTG GCCAGCTGAG CATGCGCTGC TGGTATGATA AGGATGGCCG ACTTCTGCCT 
GAGTTTAACA TGGCAGAACC ACCCTTGATC TTCGAGTGCA ATCATGCCTG CTCATGCTGG 
AGGAACTGCC GCAATCGTGT GGTGCAAAAT GGTCTCAGGG CAAGGCTGCA GCTTTATCGG 
ACACAGGACA TGGGCTGGGG TGTGCGGTCC CTCCAGGATA TCCCACTGGG CACCTTTGTC 
TGCGAATACG TAGGGGAGCT GATTTCGGAC TCTGAAGCTG ATGTTCGGGA AGAGGACTCT 
TACCTCTTTG ATCTTGACAA TAAGGATGGA GAGGTATACT GCATTGACGC TCGGTTCTAT 
GGGAATGTCA GCCGGTTCAT AAACCACCAC TGCGAACCCA ACCTTGTGCC TGTGCGAGTG 
TTCATGTCAC ACCAGGACCT GCGGTTTCCC AGGATTGCCT TCTTCAGTAC CCGCCTGATT 
CAGGCTGGGG AGCAGCTCGG GTTCGACTAC GGGGAGCGCT TTTGGGACGT CAAGGGCAAG 
CTCTTCAGTT GCCGGTGTGG GTCTTCCAAG TGTCGGCACT CAAGCGCAGC CCTGGCCCAG 
AGGCAAGCCA GTGCAGCCCA GGAGCCTCAG GAGAATGGCC TTCCAGATAC CAGCTCTGCA 
GCCGCTGCTG ACCCCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498431.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498494.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X15, mRNA.

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CloneID	OMu29809
Clone ID Related Accession (Same CDS sequence)	XM_006498431.2
Accession Version	XM_006498431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA 
AGCATGGAGC AGGCAGCTCT GGGAGACAGC GCTGGCTATA TGGAAGTTTC CCTGGATTCC 
CTGGATCTCC GTGTCAGAGG AATTCTGTCT TCCCAGACAG AAAATGAAGG GCTGGCCAGT 
GGTCCGGATG TGCTGGGGAC GGATGGCCTC CAGGAAGTGC CTCTCTGCAG CTGCCGAATG 
GAAACCCCCA AGAGCCGCGA GATCAGCACC CTGGCCAACA ACCAGTGCAT GGCCACTGAG 
AGCGTGGATC ACGAAGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498494.1
CDS	27..3821
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498431.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498431.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG TTTTAGCAGC TGCAGTATCT 
CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC CCAAGAAAAA GACCAAAGTA 
TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA CCCATTCCAC TGTGGGCGCC 
AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA ATGGCGAGAG CTTGGAGATG 
GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG AGGACCATGG AGCTGAGCAA 
GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG AGAGCATGTC TGAGACTGAC 
CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG AGTCTCCCGA CACAGGGGAG 
GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT CCAGTATCAA GAAGAAATTT 
CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC CTGCTCGGAA AAGGAGGCGG 
AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGTTCCGAGG CTTGTAAGTC ATCTCCAGGA 
AGCATGGAGC AGGCAGCTCT GGGAGACAGC GCTGGCTATA TGGAAGTTTC CCTGGATTCC 
CTGGATCTCC GTGTCAGAGG AATTCTGTCT TCCCAGACAG AAAATGAAGG GCTGGCCAGT 
GGTCCGGATG TGCTGGGGAC GGATGGCCTC CAGGAAGTGC CTCTCTGCAG CTGCCGAATG 
GAAACCCCCA AGAGCCGCGA GATCAGCACC CTGGCCAACA ACCAGTGCAT GGCCACTGAG 
AGCGTGGATC ACGAAGGTAA CTTCATGGAA TGCCAGCCTG AGAGCAGCAT CTCTCATCGT 
TTCCATAAGG ACTGTGCCTC TCGAGTCAAC AATGCCAGCT ACTGTCCCCA TTGTGGGGAA 
GAAGCTTCCA AGGCCAAAGA GGTGACCATA GCAAAAGCAG ACACAACTTC CACAGTGACC 
CTAGCCCCTG GACAGGAGAA GAGCCTGGCT GCTGAAGGCA GGGCTGACAC GACCACGGGC 
AGCATTGCTG GAGCCCCAGA GGATGAAAGA TCGCAGAGCA CAGCCCCCCA GGCACCAGAG 
TGCTTCGACC CTGCCGGACC GGCTGGGCTC GTGAGGCCGA CATCTGGCCT TTCCCAGGGC 
CCAGGAAAGG AAACCTTGGA AAGTGCTCTA ATCGCTCTAG ACTCTGAAAA ACCCAAGAAA 
CTTCGCTTCC ACCCAAAGCA GCTGTACTTC TCTGCCAGGC AGGGTGAGCT GCAGAAGGTG 
CTTCTCATGC TGGTTGATGG AATTGATCCC AACTTCAAAA TGGAGCACCA AAGTAAGCGT 
TCCCCATTAC ATGCTGCTGC GGAGGCTGGC CACGTGGACA TCTGCCACAT GCTGGTTCAG 
GCGGGTGCCA ATATTGACAC TTGCTCAGAG GACCAGCGGA CCCCACTGAT GGAGGCTGCA 
GAGAACAACC ACTTGGATGC AGTGAAGTAC CTCATCAAGG CTGGAGCACA GGTGGATCCG 
AAGGACGCAG AGGGCTCCAC ATGTTTGCAT TTGGCTGCCA AGAAAGGCCA CTATGATGTG 
GTTCAGTATC TGCTTTCAAA TGGACAGATG GATGTCAACT GCCAGGATGA CGGTGGATGG 
ACACCTATGA TCTGGGCCAC TGAGTACAAG CACGTGGAGC TGGTGAAGCT GCTGCTGTCT 
AAGGGCTCTG ACATCAACAT CCGGGACAAC GAGGAGAACA TTTGTCTGCA CTGGGCAGCA 
TTTTCAGGCT GTGTGGACAT AGCTGAAATA CTTCTGGCTG CCAAGTGTGA CCTGCATGCT 
GTGAATATCC ATGGAGACTC ACCCCTGCAC ATCGCAGCCA GGGAGAATCG CTACGACTGT 
GTTGTCCTCT TTCTTTCTCG GGATTCAGAT GTTACTCTGA AAAACAAGGA AGGAGAGACT 
CCCTTGCAGT GTGCAAGTCT CAGTTCGCAG GTGTGGAGTG CATTGCAGAT GAGCAAAGCA 
CTTCGGGACT CAGCCCCTGA CAAGCCCGTT GCTGTTGAGA AGACGGTGAG CAGGGATATC 
GCTCGAGGGT ATGAGCGCAT TCCCATTCCC TGTGTCAATG CTGTGGACAG TGAGCTGTGT 
CCTACCAACT ATAAGTATGT CTCCCAGAAC TGTGTGACAT CCCCCATGAA CATTGACAGG 
AACATCACTC ATTTGCAGTA CTGCGTGTGT GTAGATGACT GCTCCTCTAG CACCTGCATG 
TGTGGCCAGC TGAGCATGCG CTGCTGGTAT GATAAGGATG GCCGACTTCT GCCTGAGTTT 
AACATGGCAG AACCACCCTT GATCTTCGAG TGCAATCATG CCTGCTCATG CTGGAGGAAC 
TGCCGCAATC GTGTGGTGCA AAATGGTCTC AGGGCAAGGC TGCAGCTTTA TCGGACACAG 
GACATGGGCT GGGGTGTGCG GTCCCTCCAG GATATCCCAC TGGGCACCTT TGTCTGCGAA 
TACGTAGGGG AGCTGATTTC GGACTCTGAA GCTGATGTTC GGGAAGAGGA CTCTTACCTC 
TTTGATCTTG ACAATAAGGA TGGAGAGGTA TACTGCATTG ACGCTCGGTT CTATGGGAAT 
GTCAGCCGGT TCATAAACCA CCACTGCGAA CCCAACCTTG TGCCTGTGCG AGTGTTCATG 
TCACACCAGG ACCTGCGGTT TCCCAGGATT GCCTTCTTCA GTACCCGCCT GATTCAGGCT 
GGGGAGCAGC TCGGGTTCGA CTACGGGGAG CGCTTTTGGG ACGTCAAGGG CAAGCTCTTC 
AGTTGCCGGT GTGGGTCTTC CAAGTGTCGG CACTCAAGCG CAGCCCTGGC CCAGAGGCAA 
GCCAGTGCAG CCCAGGAGCC TCAGGAGAAT GGCCTTCCAG ATACCAGCTC TGCAGCCGCT 
GCTGACCCCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006498432.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498495.1 Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X18, mRNA.

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CloneID	OMu29810
Clone ID Related Accession (Same CDS sequence)	XM_006498432.2
Accession Version	XM_006498432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase EHMT1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GGCTGGCCAG TGGTCCGGAT GTGCTGGGGA CGGATGGCCT CCAGGAAGTG 
CCTCTCTGCA GCTGCCGAAT GGAAACCCCC AAGAGCCGCG AGATCAGCAC CCTGGCCAAC 
AACCAGTGCA TGGCCACTGA GAGCGTGGAT CACGAATTGG GCCGGTGTAC AAACAGCGTG 
GTCAAGTATG AGCTGATGCG CCCATCCAAC AAAGCACCGC TCTTGGTGTT ATGTGAAGAC 
CATCGGGGTC GCATGGTGAA GCACCAGTGC TGTCCTGGCT GCGGCTACTT CTGCACGGCG 
GGTAACTTCA TGGAATGCCA GCCTGAGAGC AGCATCTCTC ATCGTTTCCA TAAGGACTGT 
GCCTCTCGAG TCAACAATGC CAGCTACTGT CCCCATTGTG GGGAAGAAGC TTCCAAGGCC 
AAAGAGGTGA CCATAGCAAA AGCAGACACA ACTTCCACAG TGACCCTAGC CCCTGGACAG 
GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT GACACGACCA CGGGCAGCAT TGCTGGAGCC 
CCAGAGGATG AAAGATCGCA GAGCACAGCC CCCCAGGCAC CAGAGTGCTT CGACCCTGCC 
GGACCGGCTG GGCTCGTGAG GCCGACATCT GGCCTTTCCC AGGGCCCAGG AAAGGAAACC 
TTGGAAAGTG CTCTAATCGC TCTAGACTCT GAAAAACCCA AGAAACTTCG CTTCCACCCA 
AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT GAGCTGCAGA AGGTGCTTCT CATGCTGGTT 
GATGGAATTG ATCCCAACTT CAAAATGGAG CACCAAAGTA AGCGTTCCCC ATTACATGCT 
GCTGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGG TTCAGGCGGG TGCCAATATT 
GACACTTGCT CAGAGGACCA GCGGACCCCA CTGATGGAGG CTGCAGAGAA CAACCACTTG 
GATGCAGTGA AGTACCTCAT CAAGGCTGGA GCACAGGTGG ATCCGAAGGA CGCAGAGGGC 
TCCACATGTT TGCATTTGGC TGCCAAGAAA GGCCACTATG ATGTGGTTCA GTATCTGCTT 
TCAAATGGAC AGATGGATGT CAACTGCCAG GATGACGGTG GATGGACACC TATGATCTGG 
GCCACTGAGT ACAAGCACGT GGAGCTGGTG AAGCTGCTGC TGTCTAAGGG CTCTGACATC 
AACATCCGGG ACAACGAGGA GAACATTTGT CTGCACTGGG CAGCATTTTC AGGCTGTGTG 
GACATAGCTG AAATACTTCT GGCTGCCAAG TGTGACCTGC ATGCTGTGAA TATCCATGGA 
GACTCACCCC TGCACATCGC AGCCAGGGAG AATCGCTACG ACTGTGTTGT CCTCTTTCTT 
TCTCGGGATT CAGATGTTAC TCTGAAAAAC AAGGAAGGAG AGACTCCCTT GCAGTGTGCA 
AGTCTCAGTT CGCAGGTGTG GAGTGCATTG CAGATGAGCA AAGCACTTCG GGACTCAGCC 
CCTGACAAGC CCGTTGCTGT TGAGAAGACG GTGAGCAGGG ATATCGCTCG AGGGTATGAG 
CGCATTCCCA TTCCCTGTGT CAATGCTGTG GACAGTGAGC TGTGTCCTAC CAACTATAAG 
TATGTCTCCC AGAACTGTGT GACATCCCCC ATGAACATTG ACAGGAACAT CACTCATTTG 
CAGTACTGCG TGTGTGTAGA TGACTGCTCC TCTAGCACCT GCATGTGTGG CCAGCTGAGC 
ATGCGCTGCT GGTATGATAA GGATGGCCGA CTTCTGCCTG AGTTTAACAT GGCAGAACCA 
CCCTTGATCT TCGAGTGCAA TCATGCCTGC TCATGCTGGA GGAACTGCCG CAATCGTGTG 
GTGCAAAATG GTCTCAGGGC AAGGCTGCAG CTTTATCGGA CACAGGACAT GGGCTGGGGT 
GTGCGGTCCC TCCAGGATAT CCCACTGGGC ACCTTTGTCT GCGAATACGT AGGGGAGCTG 
ATTTCGGACT CTGAAGCTGA TGTTCGGGAA GAGGACTCTT ACCTCTTTGA TCTTGACAAT 
AAGGATGGAG AGGTATACTG CATTGACGCT CGGTTCTATG GGAATGTCAG CCGGTTCATA 
AACCACCACT GCGAACCCAA CCTTGTGCCT GTGCGAGTGT TCATGTCACA CCAGGACCTG 
CGGTTTCCCA GGATTGCCTT CTTCAGTACC CGCCTGATTC AGGCTGGGGA GCAGCTCGGG 
TTCGACTACG GGGAGCGCTT TTGGGACGTC AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG 
TCTTCCAAGT GTCGGCACTC AAGCGCAGCC CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG 
GAGCCTCAGG AGAATGGCCT TCCAGATACC AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498495.1
CDS	27..3806
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREGKDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006498432.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus euchromatic histone methyltransferase 1 (Ehmt1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498432.2

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCTGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAGCTG 
CTAAGGGAAG GTAAAGATAC ACCTATGGCT GCTGATGAAG GTTCCACAGA GAAACAAGAA 
GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT CTTGTGAAAA GAGTGGGGAT 
CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA CAAGAGCTAG CCCACAGGAA 
GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT CAGAAAGAGA CACAGAAGTG 
GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA CATCTGTCAT TGGCAGCAAT 
GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT TGAGGACTCC CAACATTCTA 
ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG GAGGAGCCAG TAAATGCAGG 
ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA CTGTGCCTGG GGAAGGGAGT 
GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG ATGTCAGGGT TCACAGGGCA 
CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG CCAGCAAAGA CCATAGAGAA 
GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA ACATTTCTGA ATGTGGACGA 
CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC TACCTCAGAA TCAGTGCTAC 
ATGGCCACCA CAAAGTCCCA GACAGCAGCT GCAGTATCTC GGAAGAAAAA ACGAAGAATG 
GGAACCTATA GTTTAGTTCC CAAGAAAAAG ACCAAAGTAT TAAAACAGAG GACGGTGATT 
GAGATGTTTA AAAGCATAAC CCATTCCACT GTGGGCGCCA AGGGCGAGAA AGCCTTAGAT 
GATAGTGCCC TGCATGTAAA TGGCGAGAGC TTGGAGATGG ACTCAGAAGA TGAAGACTCC 
GATGAGCTGG AGGATGACGA GGACCATGGA GCTGAGCAAG CGGCTGCATT TCCCACCGAG 
GATAGTAGGA CTTCTAAAGA GAGCATGTCT GAGACTGACC GGGCTGCAAA GATGGATGGA 
GATTCAGAGG AGGAACAGGA GTCTCCCGAC ACAGGGGAGG ATGAAGATGG TGGAGACGAG 
TCTGACCTGA GTTCTGAATC CAGTATCAAG AAGAAATTTC TCAAGAGGAG AGGGAAGACT 
GACAGCCCCT GGATCAAACC TGCTCGGAAA AGGAGGCGGA GAAGTAGAAA GAAGCCGAGC 
AGCATGCTTG GGCTGGCCAG TGGTCCGGAT GTGCTGGGGA CGGATGGCCT CCAGGAAGTG 
CCTCTCTGCA GCTGCCGAAT GGAAACCCCC AAGAGCCGCG AGATCAGCAC CCTGGCCAAC 
AACCAGTGCA TGGCCACTGA GAGCGTGGAT CACGAATTGG GCCGGTGTAC AAACAGCGTG 
GTCAAGTATG AGCTGATGCG CCCATCCAAC AAAGCACCGC TCTTGGTGTT ATGTGAAGAC 
CATCGGGGTC GCATGGTGAA GCACCAGTGC TGTCCTGGCT GCGGCTACTT CTGCACGGCG 
GGTAACTTCA TGGAATGCCA GCCTGAGAGC AGCATCTCTC ATCGTTTCCA TAAGGACTGT 
GCCTCTCGAG TCAACAATGC CAGCTACTGT CCCCATTGTG GGGAAGAAGC TTCCAAGGCC 
AAAGAGGTGA CCATAGCAAA AGCAGACACA ACTTCCACAG TGACCCTAGC CCCTGGACAG 
GAGAAGAGCC TGGCTGCTGA AGGCAGGGCT GACACGACCA CGGGCAGCAT TGCTGGAGCC 
CCAGAGGATG AAAGATCGCA GAGCACAGCC CCCCAGGCAC CAGAGTGCTT CGACCCTGCC 
GGACCGGCTG GGCTCGTGAG GCCGACATCT GGCCTTTCCC AGGGCCCAGG AAAGGAAACC 
TTGGAAAGTG CTCTAATCGC TCTAGACTCT GAAAAACCCA AGAAACTTCG CTTCCACCCA 
AAGCAGCTGT ACTTCTCTGC CAGGCAGGGT GAGCTGCAGA AGGTGCTTCT CATGCTGGTT 
GATGGAATTG ATCCCAACTT CAAAATGGAG CACCAAAGTA AGCGTTCCCC ATTACATGCT 
GCTGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGG TTCAGGCGGG TGCCAATATT 
GACACTTGCT CAGAGGACCA GCGGACCCCA CTGATGGAGG CTGCAGAGAA CAACCACTTG 
GATGCAGTGA AGTACCTCAT CAAGGCTGGA GCACAGGTGG ATCCGAAGGA CGCAGAGGGC 
TCCACATGTT TGCATTTGGC TGCCAAGAAA GGCCACTATG ATGTGGTTCA GTATCTGCTT 
TCAAATGGAC AGATGGATGT CAACTGCCAG GATGACGGTG GATGGACACC TATGATCTGG 
GCCACTGAGT ACAAGCACGT GGAGCTGGTG AAGCTGCTGC TGTCTAAGGG CTCTGACATC 
AACATCCGGG ACAACGAGGA GAACATTTGT CTGCACTGGG CAGCATTTTC AGGCTGTGTG 
GACATAGCTG AAATACTTCT GGCTGCCAAG TGTGACCTGC ATGCTGTGAA TATCCATGGA 
GACTCACCCC TGCACATCGC AGCCAGGGAG AATCGCTACG ACTGTGTTGT CCTCTTTCTT 
TCTCGGGATT CAGATGTTAC TCTGAAAAAC AAGGAAGGAG AGACTCCCTT GCAGTGTGCA 
AGTCTCAGTT CGCAGGTGTG GAGTGCATTG CAGATGAGCA AAGCACTTCG GGACTCAGCC 
CCTGACAAGC CCGTTGCTGT TGAGAAGACG GTGAGCAGGG ATATCGCTCG AGGGTATGAG 
CGCATTCCCA TTCCCTGTGT CAATGCTGTG GACAGTGAGC TGTGTCCTAC CAACTATAAG 
TATGTCTCCC AGAACTGTGT GACATCCCCC ATGAACATTG ACAGGAACAT CACTCATTTG 
CAGTACTGCG TGTGTGTAGA TGACTGCTCC TCTAGCACCT GCATGTGTGG CCAGCTGAGC 
ATGCGCTGCT GGTATGATAA GGATGGCCGA CTTCTGCCTG AGTTTAACAT GGCAGAACCA 
CCCTTGATCT TCGAGTGCAA TCATGCCTGC TCATGCTGGA GGAACTGCCG CAATCGTGTG 
GTGCAAAATG GTCTCAGGGC AAGGCTGCAG CTTTATCGGA CACAGGACAT GGGCTGGGGT 
GTGCGGTCCC TCCAGGATAT CCCACTGGGC ACCTTTGTCT GCGAATACGT AGGGGAGCTG 
ATTTCGGACT CTGAAGCTGA TGTTCGGGAA GAGGACTCTT ACCTCTTTGA TCTTGACAAT 
AAGGATGGAG AGGTATACTG CATTGACGCT CGGTTCTATG GGAATGTCAG CCGGTTCATA 
AACCACCACT GCGAACCCAA CCTTGTGCCT GTGCGAGTGT TCATGTCACA CCAGGACCTG 
CGGTTTCCCA GGATTGCCTT CTTCAGTACC CGCCTGATTC AGGCTGGGGA GCAGCTCGGG 
TTCGACTACG GGGAGCGCTT TTGGGACGTC AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG 
TCTTCCAAGT GTCGGCACTC AAGCGCAGCC CTGGCCCAGA GGCAAGCCAG TGCAGCCCAG 
GAGCCTCAGG AGAATGGCCT TCCAGATACC AGCTCTGCAG CCGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Repression of Adipose Tissue Fibrosis through a PRDM16-GTF2IRD1 Complex Improves Systemic Glucose Homeostasis.
Cell metabolism27(1)180-194.e6(2018 Jan)
Hasegawa Y,Ikeda K,Chen Y,Alba DL,Stifler D,Shinoda K,Hosono T,Maretich P,Yang Y,Ishigaki Y,Chi J,Cohen P,Koliwad SK,Kajimura S

Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)
Hatazawa Y,Ono Y,Hirose Y,Kanai S,Fujii NL,Machida S,Nishino I,Shimizu T,Okano M,Kamei Y,Ogawa Y

GLP inhibits heterochromatin clustering and myogenic differentiation by repressing MeCP2.
Journal of molecular cell biology(2017 Aug)
Choi MH,Palanichamy Kala M,Ow JR,Rao VK,Suriyamurthy S,Taneja R

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Ehmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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