ehmt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ehmt1
Entrez Gene ID	100124713
Full Name	euchromatic histone-lysine N-methyltransferase 1
General protein information	Preferred Names euchromatic histone-lysine N-methyltransferase 1 Names histone-lysine N-methyltransferase EHMT1 histone-lysine N-methyltransferase, H3 lysine-9 specific 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102698.1	NP_001096168.1	histone-lysine N-methyltransferase EHMT1
XM_031906833.1	XP_031762693.1	histone-lysine N-methyltransferase EHMT1 isoform X2
XM_031906834.1	XP_031762694.1	histone-lysine N-methyltransferase EHMT1 isoform X3
XM_031906837.1	XP_031762697.1	histone-lysine N-methyltransferase EHMT1 isoform X4
XM_031906832.1	XP_031762692.1	histone-lysine N-methyltransferase EHMT1 isoform X1
XM_031906838.1	XP_031762698.1	histone-lysine N-methyltransferase EHMT1 isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	EHMT1	NP_079033.4
Pan troglodytes (chimpanzee)	EHMT1	XP_520395.3
Bos taurus (cattle)	EHMT1	NP_001092511.1
Mus musculus (house mouse)	Ehmt1	NP_001012536.2
Rattus norvegicus (Norway rat)	Ehmt1	NP_001102042.1
Gallus gallus (chicken)	EHMT1	NP_001012550.1
Xenopus tropicalis (tropical clawed frog)	ehmt1	NP_001096168.1
Danio rerio (zebrafish)	ehmt1b	NP_001166035.1
Canis lupus familiaris (dog)	EHMT1	XP_005625091.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ehmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ehmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63378	XM_031906832.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63379	XM_031906833.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63380	XM_031906834.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63381	XM_031906837.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63382	XM_031906838.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa01844	NM_001102698.1 Latest version!	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ehmt1 ( XM_031906832.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762692.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63378
Clone ID Related Accession (Same CDS sequence)	XM_031906832.1
Accession Version	XM_031906832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC TGGATGCTGA GCGTGTCGAT ACAAGCCAGA CGGAGTCCAG GAACGACTTA 
TTCCTGAAGA CGGACTTGTT GAAGGCGATA GCCAACAGCC AAGAGGAAGT ATCTGAAGCC 
ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCA GCTGGGTCGC TGTACCAACA GGGTGGCAAA GTACGAGCTA 
ATGAGGCCTT CTAACAAGGT GCGTCATCTG GTGCTGTGTG AGGATCACCG GGGCAAGATG 
GTCAAACACC AGTGTTGTCC TGGCTGCGGT TACTTCTGCA CAGCGGGGAC CTTCATGGAA 
TGCCAGCCGG ACAGCACCAT CTCCCACCGA TTCCACAAGG ACTGCGCCAC CCGGGTCAAC 
AACTCGAGCT ACTGCCCACA CTGCGGGGAG GAGGCCTCCA AAGCGAAGGA GGTGACCATC 
GCCAAGGCCG ACACCACGTC CTCCCTGGCC CCAGTGACCG GCGAATGGGA GAAAGTCAGT 
GCGGCAGAGG GACAGGCGGA CACTACAACC GAGAGCGAGG CCATGGCTCT AAAGGCGCCA 
GAGGAGAATA AGCAAGAAGC AACATCTTCC CTCCCTGGAG GAACCCCTGA GCCTATAGAG 
ACCCCAAGTT TGGGCAAACC ACCCTCTCTG TCTCAGGGGC CCGTCAAGGA GACCTTGGAA 
AGCGCTCTAC TCGCTTTGGA CTCTGAAAAA CCCAAGAAAT TGCGGTTTCA CCCAAAGCAG 
CTGTATTTTT CTGCCCGGCA AGGAGAGCTG CAGAAAGTCC TGATGATGCT GGTGGACGGT 
ATTGACCCCA ACTACAAAAT GGAGCAGCAC AGTCGGAGAA CACCACTTCA CGCAGCGGCA 
GAGATGGGTT ACACGGACAT CTGCCACATG CTTATACAGG CCGGAGCCAA CCTAGACAGC 
TGCTCGGAGG ATCAACGCAC CCCCCTGATG GAGGCAGCTG AAAATAATCA TCTTGATACT 
GTGAAATACC TGGTGAAGGG CGGAGCTCTA ATTGACCCTA AGGACTCCGA GGGCTCCACT 
TGTCTTCATC TGGCGGCCAA GAAGGGTCAC TACGAAGTGG TGAAATATCT CCTCTCCTCC 
GAGAGAACCG ACGTCAACTG CCAGGACGAC GGGGGCTGGA CCCCGATGAT CTGGGCTACC 
GAGTACAAGC ACGTGGAGAT AGTCAAACTG TTACATTCCT CCCGCGCGGA CGTCAATATC 
CGCGATAATG AGGAGAACAT CTGCCTGCAC TGGGCCGCCT TCGCCGGTTC CGTCGAGATA 
GCGGAAATCC TCCTGGCTTC CAAATGTGAC CTCCGAGCTG TGAATATCCA CGGGGACTCC 
CCGCTCCATA TTGCCGCCCG CGAGAACCGC CACGAGTGCG TCATGCTGTT CCTGGGTCAC 
GGCTCGGAAG TGGGACTGAA GAACAAAGAG GGCGAGACGC CGCTGGAGTG CTCGGCGCCC 
AACTCCGACG TTTGGGCGGC CCTTAGCAAG TGCCAGGCCT TGCCCGAGAA GCCCACGCTG 
CAGGAAAAGG TGGTGGACAG GGATATATCC CGGGGGTACG AGAATATCCC CATCCCTTGC 
GTCAACGCCG AAGACTCCGA GCTGTGTCCC GTCAACTACA AATACGTCTC TCAGAACTGC 
GTGACCTCCC CGCTGAACAT CGACAGAAAC ATTACCCATC TGCAGTACTG TGTGTGCATC 
GATGACTGTT CCTCTAGTAA CTGTATGTGC GGCCAGCTGA GTATGAGGTG CTGGTACGAC 
AAGAACGGGC GCTTGTTACC AGAGTTCAAC ATGTTGGAGC CCCCACTGAT CTTCGAATGC 
AACCACGCCT GCTCCTGCTG GCGCAACTGC CGCAACCGTG TGGTTCAAAA TGGCTTGAAG 
ATTAGATTGC AACTGTTCCG CACCAAAAGC AAAGGCTGGG GGGTGCGATC CCTGCAAGAC 
ATTCCCCAGG GGACCTTCGT ATGCGAGTAT GTGGGGGAGC TGATTTCGGA CGCAGAGGCT 
GACGTTCGAG AGGATGACAC CTACCTCTTT GATCTTGATA ACAAGGACCG AGAGGTTTAC 
TGCATCGACG CCCGTTTCTA CGGCAACATC AGTCGCTTTA TCAACCACCT CTGCGAGCCG 
AACCTGCTCC CCGTGCGAGT CTTTATGTCC CACCAGGATC TGCGCTTTCC CCGCATCGGC 
TTCTTCAGCA GCCGGCACAT CGGCGCGGGG GAGGAGATCG GGTTTGATTA CGGCGACCGA 
TTCTGGGACG TCAAGGGGAA GCTCTTCAGC TGCCAGTGCG GTTCCCCCAA GTGCAAGCAC 
CCAATCAAAG TCCTTTCCCC GAGCCAGTCA TTCACCCCCG CAGAAACTCC GTCGGAGATT 
GGCTTACCCG ACACCAGCTC GGCCACCCCC CCGAGCCCCT CGCAGCCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762692.1
CDS	80..3913
Translation	MATLDAERVDTSQTESRNDLFLKTDLLKAIANSQEEVSEAMAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPFKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQEQLGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFTPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	XM_031906832.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906832.1

ORF Insert Sequence:

ATGGCCACAC TGGATGCTGA GCGTGTCGAT ACAAGCCAGA CGGAGTCCAG GAACGACTTA 
TTCCTGAAGA CGGACTTGTT GAAGGCGATA GCCAACAGCC AAGAGGAAGT ATCTGAAGCC 
ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCA GCTGGGTCGC TGTACCAACA GGGTGGCAAA GTACGAGCTA 
ATGAGGCCTT CTAACAAGGT GCGTCATCTG GTGCTGTGTG AGGATCACCG GGGCAAGATG 
GTCAAACACC AGTGTTGTCC TGGCTGCGGT TACTTCTGCA CAGCGGGGAC CTTCATGGAA 
TGCCAGCCGG ACAGCACCAT CTCCCACCGA TTCCACAAGG ACTGCGCCAC CCGGGTCAAC 
AACTCGAGCT ACTGCCCACA CTGCGGGGAG GAGGCCTCCA AAGCGAAGGA GGTGACCATC 
GCCAAGGCCG ACACCACGTC CTCCCTGGCC CCAGTGACCG GCGAATGGGA GAAAGTCAGT 
GCGGCAGAGG GACAGGCGGA CACTACAACC GAGAGCGAGG CCATGGCTCT AAAGGCGCCA 
GAGGAGAATA AGCAAGAAGC AACATCTTCC CTCCCTGGAG GAACCCCTGA GCCTATAGAG 
ACCCCAAGTT TGGGCAAACC ACCCTCTCTG TCTCAGGGGC CCGTCAAGGA GACCTTGGAA 
AGCGCTCTAC TCGCTTTGGA CTCTGAAAAA CCCAAGAAAT TGCGGTTTCA CCCAAAGCAG 
CTGTATTTTT CTGCCCGGCA AGGAGAGCTG CAGAAAGTCC TGATGATGCT GGTGGACGGT 
ATTGACCCCA ACTACAAAAT GGAGCAGCAC AGTCGGAGAA CACCACTTCA CGCAGCGGCA 
GAGATGGGTT ACACGGACAT CTGCCACATG CTTATACAGG CCGGAGCCAA CCTAGACAGC 
TGCTCGGAGG ATCAACGCAC CCCCCTGATG GAGGCAGCTG AAAATAATCA TCTTGATACT 
GTGAAATACC TGGTGAAGGG CGGAGCTCTA ATTGACCCTA AGGACTCCGA GGGCTCCACT 
TGTCTTCATC TGGCGGCCAA GAAGGGTCAC TACGAAGTGG TGAAATATCT CCTCTCCTCC 
GAGAGAACCG ACGTCAACTG CCAGGACGAC GGGGGCTGGA CCCCGATGAT CTGGGCTACC 
GAGTACAAGC ACGTGGAGAT AGTCAAACTG TTACATTCCT CCCGCGCGGA CGTCAATATC 
CGCGATAATG AGGAGAACAT CTGCCTGCAC TGGGCCGCCT TCGCCGGTTC CGTCGAGATA 
GCGGAAATCC TCCTGGCTTC CAAATGTGAC CTCCGAGCTG TGAATATCCA CGGGGACTCC 
CCGCTCCATA TTGCCGCCCG CGAGAACCGC CACGAGTGCG TCATGCTGTT CCTGGGTCAC 
GGCTCGGAAG TGGGACTGAA GAACAAAGAG GGCGAGACGC CGCTGGAGTG CTCGGCGCCC 
AACTCCGACG TTTGGGCGGC CCTTAGCAAG TGCCAGGCCT TGCCCGAGAA GCCCACGCTG 
CAGGAAAAGG TGGTGGACAG GGATATATCC CGGGGGTACG AGAATATCCC CATCCCTTGC 
GTCAACGCCG AAGACTCCGA GCTGTGTCCC GTCAACTACA AATACGTCTC TCAGAACTGC 
GTGACCTCCC CGCTGAACAT CGACAGAAAC ATTACCCATC TGCAGTACTG TGTGTGCATC 
GATGACTGTT CCTCTAGTAA CTGTATGTGC GGCCAGCTGA GTATGAGGTG CTGGTACGAC 
AAGAACGGGC GCTTGTTACC AGAGTTCAAC ATGTTGGAGC CCCCACTGAT CTTCGAATGC 
AACCACGCCT GCTCCTGCTG GCGCAACTGC CGCAACCGTG TGGTTCAAAA TGGCTTGAAG 
ATTAGATTGC AACTGTTCCG CACCAAAAGC AAAGGCTGGG GGGTGCGATC CCTGCAAGAC 
ATTCCCCAGG GGACCTTCGT ATGCGAGTAT GTGGGGGAGC TGATTTCGGA CGCAGAGGCT 
GACGTTCGAG AGGATGACAC CTACCTCTTT GATCTTGATA ACAAGGACCG AGAGGTTTAC 
TGCATCGACG CCCGTTTCTA CGGCAACATC AGTCGCTTTA TCAACCACCT CTGCGAGCCG 
AACCTGCTCC CCGTGCGAGT CTTTATGTCC CACCAGGATC TGCGCTTTCC CCGCATCGGC 
TTCTTCAGCA GCCGGCACAT CGGCGCGGGG GAGGAGATCG GGTTTGATTA CGGCGACCGA 
TTCTGGGACG TCAAGGGGAA GCTCTTCAGC TGCCAGTGCG GTTCCCCCAA GTGCAAGCAC 
CCAATCAAAG TCCTTTCCCC GAGCCAGTCA TTCACCCCCG CAGAAACTCC GTCGGAGATT 
GGCTTACCCG ACACCAGCTC GGCCACCCCC CCGAGCCCCT CGCAGCCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1 ( XM_031906833.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762693.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63379
Clone ID Related Accession (Same CDS sequence)	XM_031906833.1
Accession Version	XM_031906833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC TGGATGCTGA GCGTGTCGAT ACAAGCCAGA CGGAGTCCAG GAACGACTTA 
TTCCTGAAGA CGGACTTGTT GAAGGCGATA GCCAACAGCC AAGAGGAAGT ATCTGAAGCC 
ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCT GGGTCGCTGT ACCAACAGGG TGGCAAAGTA CGAGCTAATG 
AGGCCTTCTA ACAAGGTGCG TCATCTGGTG CTGTGTGAGG ATCACCGGGG CAAGATGGTC 
AAACACCAGT GTTGTCCTGG CTGCGGTTAC TTCTGCACAG CGGGGACCTT CATGGAATGC 
CAGCCGGACA GCACCATCTC CCACCGATTC CACAAGGACT GCGCCACCCG GGTCAACAAC 
TCGAGCTACT GCCCACACTG CGGGGAGGAG GCCTCCAAAG CGAAGGAGGT GACCATCGCC 
AAGGCCGACA CCACGTCCTC CCTGGCCCCA GTGACCGGCG AATGGGAGAA AGTCAGTGCG 
GCAGAGGGAC AGGCGGACAC TACAACCGAG AGCGAGGCCA TGGCTCTAAA GGCGCCAGAG 
GAGAATAAGC AAGAAGCAAC ATCTTCCCTC CCTGGAGGAA CCCCTGAGCC TATAGAGACC 
CCAAGTTTGG GCAAACCACC CTCTCTGTCT CAGGGGCCCG TCAAGGAGAC CTTGGAAAGC 
GCTCTACTCG CTTTGGACTC TGAAAAACCC AAGAAATTGC GGTTTCACCC AAAGCAGCTG 
TATTTTTCTG CCCGGCAAGG AGAGCTGCAG AAAGTCCTGA TGATGCTGGT GGACGGTATT 
GACCCCAACT ACAAAATGGA GCAGCACAGT CGGAGAACAC CACTTCACGC AGCGGCAGAG 
ATGGGTTACA CGGACATCTG CCACATGCTT ATACAGGCCG GAGCCAACCT AGACAGCTGC 
TCGGAGGATC AACGCACCCC CCTGATGGAG GCAGCTGAAA ATAATCATCT TGATACTGTG 
AAATACCTGG TGAAGGGCGG AGCTCTAATT GACCCTAAGG ACTCCGAGGG CTCCACTTGT 
CTTCATCTGG CGGCCAAGAA GGGTCACTAC GAAGTGGTGA AATATCTCCT CTCCTCCGAG 
AGAACCGACG TCAACTGCCA GGACGACGGG GGCTGGACCC CGATGATCTG GGCTACCGAG 
TACAAGCACG TGGAGATAGT CAAACTGTTA CATTCCTCCC GCGCGGACGT CAATATCCGC 
GATAATGAGG AGAACATCTG CCTGCACTGG GCCGCCTTCG CCGGTTCCGT CGAGATAGCG 
GAAATCCTCC TGGCTTCCAA ATGTGACCTC CGAGCTGTGA ATATCCACGG GGACTCCCCG 
CTCCATATTG CCGCCCGCGA GAACCGCCAC GAGTGCGTCA TGCTGTTCCT GGGTCACGGC 
TCGGAAGTGG GACTGAAGAA CAAAGAGGGC GAGACGCCGC TGGAGTGCTC GGCGCCCAAC 
TCCGACGTTT GGGCGGCCCT TAGCAAGTGC CAGGCCTTGC CCGAGAAGCC CACGCTGCAG 
GAAAAGGTGG TGGACAGGGA TATATCCCGG GGGTACGAGA ATATCCCCAT CCCTTGCGTC 
AACGCCGAAG ACTCCGAGCT GTGTCCCGTC AACTACAAAT ACGTCTCTCA GAACTGCGTG 
ACCTCCCCGC TGAACATCGA CAGAAACATT ACCCATCTGC AGTACTGTGT GTGCATCGAT 
GACTGTTCCT CTAGTAACTG TATGTGCGGC CAGCTGAGTA TGAGGTGCTG GTACGACAAG 
AACGGGCGCT TGTTACCAGA GTTCAACATG TTGGAGCCCC CACTGATCTT CGAATGCAAC 
CACGCCTGCT CCTGCTGGCG CAACTGCCGC AACCGTGTGG TTCAAAATGG CTTGAAGATT 
AGATTGCAAC TGTTCCGCAC CAAAAGCAAA GGCTGGGGGG TGCGATCCCT GCAAGACATT 
CCCCAGGGGA CCTTCGTATG CGAGTATGTG GGGGAGCTGA TTTCGGACGC AGAGGCTGAC 
GTTCGAGAGG ATGACACCTA CCTCTTTGAT CTTGATAACA AGGACCGAGA GGTTTACTGC 
ATCGACGCCC GTTTCTACGG CAACATCAGT CGCTTTATCA ACCACCTCTG CGAGCCGAAC 
CTGCTCCCCG TGCGAGTCTT TATGTCCCAC CAGGATCTGC GCTTTCCCCG CATCGGCTTC 
TTCAGCAGCC GGCACATCGG CGCGGGGGAG GAGATCGGGT TTGATTACGG CGACCGATTC 
TGGGACGTCA AGGGGAAGCT CTTCAGCTGC CAGTGCGGTT CCCCCAAGTG CAAGCACCCA 
ATCAAAGTCC TTTCCCCGAG CCAGTCATTC ACCCCCGCAG AAACTCCGTC GGAGATTGGC 
TTACCCGACA CCAGCTCGGC CACCCCCCCG AGCCCCTCGC AGCCGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762693.1
CDS	80..3910
Translation	MATLDAERVDTSQTESRNDLFLKTDLLKAIANSQEEVSEAMAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPFKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQELGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFTPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	XM_031906833.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906833.1

ORF Insert Sequence:

ATGGCCACAC TGGATGCTGA GCGTGTCGAT ACAAGCCAGA CGGAGTCCAG GAACGACTTA 
TTCCTGAAGA CGGACTTGTT GAAGGCGATA GCCAACAGCC AAGAGGAAGT ATCTGAAGCC 
ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCT GGGTCGCTGT ACCAACAGGG TGGCAAAGTA CGAGCTAATG 
AGGCCTTCTA ACAAGGTGCG TCATCTGGTG CTGTGTGAGG ATCACCGGGG CAAGATGGTC 
AAACACCAGT GTTGTCCTGG CTGCGGTTAC TTCTGCACAG CGGGGACCTT CATGGAATGC 
CAGCCGGACA GCACCATCTC CCACCGATTC CACAAGGACT GCGCCACCCG GGTCAACAAC 
TCGAGCTACT GCCCACACTG CGGGGAGGAG GCCTCCAAAG CGAAGGAGGT GACCATCGCC 
AAGGCCGACA CCACGTCCTC CCTGGCCCCA GTGACCGGCG AATGGGAGAA AGTCAGTGCG 
GCAGAGGGAC AGGCGGACAC TACAACCGAG AGCGAGGCCA TGGCTCTAAA GGCGCCAGAG 
GAGAATAAGC AAGAAGCAAC ATCTTCCCTC CCTGGAGGAA CCCCTGAGCC TATAGAGACC 
CCAAGTTTGG GCAAACCACC CTCTCTGTCT CAGGGGCCCG TCAAGGAGAC CTTGGAAAGC 
GCTCTACTCG CTTTGGACTC TGAAAAACCC AAGAAATTGC GGTTTCACCC AAAGCAGCTG 
TATTTTTCTG CCCGGCAAGG AGAGCTGCAG AAAGTCCTGA TGATGCTGGT GGACGGTATT 
GACCCCAACT ACAAAATGGA GCAGCACAGT CGGAGAACAC CACTTCACGC AGCGGCAGAG 
ATGGGTTACA CGGACATCTG CCACATGCTT ATACAGGCCG GAGCCAACCT AGACAGCTGC 
TCGGAGGATC AACGCACCCC CCTGATGGAG GCAGCTGAAA ATAATCATCT TGATACTGTG 
AAATACCTGG TGAAGGGCGG AGCTCTAATT GACCCTAAGG ACTCCGAGGG CTCCACTTGT 
CTTCATCTGG CGGCCAAGAA GGGTCACTAC GAAGTGGTGA AATATCTCCT CTCCTCCGAG 
AGAACCGACG TCAACTGCCA GGACGACGGG GGCTGGACCC CGATGATCTG GGCTACCGAG 
TACAAGCACG TGGAGATAGT CAAACTGTTA CATTCCTCCC GCGCGGACGT CAATATCCGC 
GATAATGAGG AGAACATCTG CCTGCACTGG GCCGCCTTCG CCGGTTCCGT CGAGATAGCG 
GAAATCCTCC TGGCTTCCAA ATGTGACCTC CGAGCTGTGA ATATCCACGG GGACTCCCCG 
CTCCATATTG CCGCCCGCGA GAACCGCCAC GAGTGCGTCA TGCTGTTCCT GGGTCACGGC 
TCGGAAGTGG GACTGAAGAA CAAAGAGGGC GAGACGCCGC TGGAGTGCTC GGCGCCCAAC 
TCCGACGTTT GGGCGGCCCT TAGCAAGTGC CAGGCCTTGC CCGAGAAGCC CACGCTGCAG 
GAAAAGGTGG TGGACAGGGA TATATCCCGG GGGTACGAGA ATATCCCCAT CCCTTGCGTC 
AACGCCGAAG ACTCCGAGCT GTGTCCCGTC AACTACAAAT ACGTCTCTCA GAACTGCGTG 
ACCTCCCCGC TGAACATCGA CAGAAACATT ACCCATCTGC AGTACTGTGT GTGCATCGAT 
GACTGTTCCT CTAGTAACTG TATGTGCGGC CAGCTGAGTA TGAGGTGCTG GTACGACAAG 
AACGGGCGCT TGTTACCAGA GTTCAACATG TTGGAGCCCC CACTGATCTT CGAATGCAAC 
CACGCCTGCT CCTGCTGGCG CAACTGCCGC AACCGTGTGG TTCAAAATGG CTTGAAGATT 
AGATTGCAAC TGTTCCGCAC CAAAAGCAAA GGCTGGGGGG TGCGATCCCT GCAAGACATT 
CCCCAGGGGA CCTTCGTATG CGAGTATGTG GGGGAGCTGA TTTCGGACGC AGAGGCTGAC 
GTTCGAGAGG ATGACACCTA CCTCTTTGAT CTTGATAACA AGGACCGAGA GGTTTACTGC 
ATCGACGCCC GTTTCTACGG CAACATCAGT CGCTTTATCA ACCACCTCTG CGAGCCGAAC 
CTGCTCCCCG TGCGAGTCTT TATGTCCCAC CAGGATCTGC GCTTTCCCCG CATCGGCTTC 
TTCAGCAGCC GGCACATCGG CGCGGGGGAG GAGATCGGGT TTGATTACGG CGACCGATTC 
TGGGACGTCA AGGGGAAGCT CTTCAGCTGC CAGTGCGGTT CCCCCAAGTG CAAGCACCCA 
ATCAAAGTCC TTTCCCCGAG CCAGTCATTC ACCCCCGCAG AAACTCCGTC GGAGATTGGC 
TTACCCGACA CCAGCTCGGC CACCCCCCCG AGCCCCTCGC AGCCGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1 ( XM_031906834.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762694.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X3, mRNA.

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CloneID	OXa63380
Clone ID Related Accession (Same CDS sequence)	XM_031906834.1
Accession Version	XM_031906834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGC GTGTCGATAC AAGCCAGACG GAGTCCAGGA ACGACTTATT CCTGAAGACG 
GACTTGTTGA AGGCGATAGC CAACAGCCAA GAGGAAGTAT CTGAAGCCAT GGCCGGCGAC 
GAAAACTTGT CGGACAAGCA GGTCGGTGAA ACGAATGGAT CTTGTGAAAA GAACGAGTCC 
AAGGAGCTCG GTTTATTGAC GCTTGCCACC CCAGACAGCA CAAAACTCCC TCACCAGGAC 
TGTGTTTTTA AAACTTTTCG TGTGGCAGAG AACGGGGTGT ACGACCGAGA CACGGAGGGG 
CAGTACCCCT TTAAAGCGAG CAACCCCACA CAGACATCCG TAACGGGAAG TAATGGGTAC 
ATTTTAAACA AACCTGCCAT TTTGGAGCAG CCATTAAGGA CTTCAAGCTT GTCACCTCTG 
AGTGGCCATG CTGCTAAAAC TCTACCGGTT GCTGCCAGCA AACCCAAAGC AGCTTCTAAT 
GTCTACGTCC CTCCTGCCCC TTCTCCTGTC CCTCCGAACC CTGGGGATCC GGGGCGGGTT 
GAGAACACAA AGCAACAGGA CAGCGCTTTA CCTGATCTGA AAGTTCACCG TGCAAGGAAA 
ACTCTACCGA AGACAAGCGG CAGTTCGCCC ATCCATAAAG AGCCCAAAGA ACCATCCGAG 
CAGGACGAAA CCAAGGACGA GAGTGCACCC AAAAATGGTT TAGATGACGA GAAAGAGGAA 
CCCCAGTCTC CCTCCTCTGC CCCTCAACAG CTGCTACCTC AGGGTCAAAA CTCTGCCAAG 
TCGCTCTCAG CTGCACTGGT GTGTCGGAAG AAGAAACGGC GGATGGGCAT GTACAGCCTC 
GTACCCAAGA AGAAGACCAA AGTGCCAAAG CAAAGAAGCG TGCTGGAGAT GTTCCGGAAC 
ATCACCTACA CCTCTTCCAG TCCCAAGAGC GAGAAAGAGT CCGATGTTCC TCATGTTAAC 
GGGGAAAGCA TGGAGATGGA TTCCGAAGAC GAGGATTCGG AAGACATGGA AGAAGAGGAC 
TACCACAGTT CGGAGACGCC AGCCTCGGAG AGTCAGGAAG AGCCCGGTGT TCCTAAAGAG 
GCCACCTCAG AAGCCGACAG TTCCAGAAAG GTCGATGAGG TTGATTCTGA AGGAGATGAA 
GACGTGGAGT CTGGGGATTC TGTAGAGGAG GAAGATGACG GAGCAGAGTC TGATTTAAGC 
TCAGAGTCCA GTTTAAGGAA GAAGCAAGAG AAGAAGAAGA CCCTGGCCGA AAGCCCCTGG 
ATGAAGGGTT CTAGGCGACG GAAGAGAAAG AACAAGGAAA ATCCCAATCC GCTTCTAGCT 
TCATCGGGAG AGGCTCGGGA AGCCAGCATG GAATACACAG AGGTCTCATT AGACTCCTTG 
GATCTGCGCG TCAAAGAGAT GTTATCTCCG CAGTCTAACG ATCTCTCTAA TGGCTCGGAG 
GCCATGGAAC TGGACGGCCT TCAGGGGATC CCCCTCTGCA GCTGCCGGAT GGAGACCCCC 
AAAGAAGAGG AGGTGTCGGC AAAGGCTGGA GAGCATTGCA TGGCCATTGA GAGCACTGGC 
CAAGAGCAGC TGGGTCGCTG TACCAACAGG GTGGCAAAGT ACGAGCTAAT GAGGCCTTCT 
AACAAGGTGC GTCATCTGGT GCTGTGTGAG GATCACCGGG GCAAGATGGT CAAACACCAG 
TGTTGTCCTG GCTGCGGTTA CTTCTGCACA GCGGGGACCT TCATGGAATG CCAGCCGGAC 
AGCACCATCT CCCACCGATT CCACAAGGAC TGCGCCACCC GGGTCAACAA CTCGAGCTAC 
TGCCCACACT GCGGGGAGGA GGCCTCCAAA GCGAAGGAGG TGACCATCGC CAAGGCCGAC 
ACCACGTCCT CCCTGGCCCC AGTGACCGGC GAATGGGAGA AAGTCAGTGC GGCAGAGGGA 
CAGGCGGACA CTACAACCGA GAGCGAGGCC ATGGCTCTAA AGGCGCCAGA GGAGAATAAG 
CAAGAAGCAA CATCTTCCCT CCCTGGAGGA ACCCCTGAGC CTATAGAGAC CCCAAGTTTG 
GGCAAACCAC CCTCTCTGTC TCAGGGGCCC GTCAAGGAGA CCTTGGAAAG CGCTCTACTC 
GCTTTGGACT CTGAAAAACC CAAGAAATTG CGGTTTCACC CAAAGCAGCT GTATTTTTCT 
GCCCGGCAAG GAGAGCTGCA GAAAGTCCTG ATGATGCTGG TGGACGGTAT TGACCCCAAC 
TACAAAATGG AGCAGCACAG TCGGAGAACA CCACTTCACG CAGCGGCAGA GATGGGTTAC 
ACGGACATCT GCCACATGCT TATACAGGCC GGAGCCAACC TAGACAGCTG CTCGGAGGAT 
CAACGCACCC CCCTGATGGA GGCAGCTGAA AATAATCATC TTGATACTGT GAAATACCTG 
GTGAAGGGCG GAGCTCTAAT TGACCCTAAG GACTCCGAGG GCTCCACTTG TCTTCATCTG 
GCGGCCAAGA AGGGTCACTA CGAAGTGGTG AAATATCTCC TCTCCTCCGA GAGAACCGAC 
GTCAACTGCC AGGACGACGG GGGCTGGACC CCGATGATCT GGGCTACCGA GTACAAGCAC 
GTGGAGATAG TCAAACTGTT ACATTCCTCC CGCGCGGACG TCAATATCCG CGATAATGAG 
GAGAACATCT GCCTGCACTG GGCCGCCTTC GCCGGTTCCG TCGAGATAGC GGAAATCCTC 
CTGGCTTCCA AATGTGACCT CCGAGCTGTG AATATCCACG GGGACTCCCC GCTCCATATT 
GCCGCCCGCG AGAACCGCCA CGAGTGCGTC ATGCTGTTCC TGGGTCACGG CTCGGAAGTG 
GGACTGAAGA ACAAAGAGGG CGAGACGCCG CTGGAGTGCT CGGCGCCCAA CTCCGACGTT 
TGGGCGGCCC TTAGCAAGTG CCAGGCCTTG CCCGAGAAGC CCACGCTGCA GGAAAAGGTG 
GTGGACAGGG ATATATCCCG GGGGTACGAG AATATCCCCA TCCCTTGCGT CAACGCCGAA 
GACTCCGAGC TGTGTCCCGT CAACTACAAA TACGTCTCTC AGAACTGCGT GACCTCCCCG 
CTGAACATCG ACAGAAACAT TACCCATCTG CAGTACTGTG TGTGCATCGA TGACTGTTCC 
TCTAGTAACT GTATGTGCGG CCAGCTGAGT ATGAGGTGCT GGTACGACAA GAACGGGCGC 
TTGTTACCAG AGTTCAACAT GTTGGAGCCC CCACTGATCT TCGAATGCAA CCACGCCTGC 
TCCTGCTGGC GCAACTGCCG CAACCGTGTG GTTCAAAATG GCTTGAAGAT TAGATTGCAA 
CTGTTCCGCA CCAAAAGCAA AGGCTGGGGG GTGCGATCCC TGCAAGACAT TCCCCAGGGG 
ACCTTCGTAT GCGAGTATGT GGGGGAGCTG ATTTCGGACG CAGAGGCTGA CGTTCGAGAG 
GATGACACCT ACCTCTTTGA TCTTGATAAC AAGGACCGAG AGGTTTACTG CATCGACGCC 
CGTTTCTACG GCAACATCAG TCGCTTTATC AACCACCTCT GCGAGCCGAA CCTGCTCCCC 
GTGCGAGTCT TTATGTCCCA CCAGGATCTG CGCTTTCCCC GCATCGGCTT CTTCAGCAGC 
CGGCACATCG GCGCGGGGGA GGAGATCGGG TTTGATTACG GCGACCGATT CTGGGACGTC 
AAGGGGAAGC TCTTCAGCTG CCAGTGCGGT TCCCCCAAGT GCAAGCACCC AATCAAAGTC 
CTTTCCCCGA GCCAGTCATT CACCCCCGCA GAAACTCCGT CGGAGATTGG CTTACCCGAC 
ACCAGCTCGG CCACCCCCCC GAGCCCCTCG CAGCCGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762694.1
CDS	16..3837
Translation	MEMRVDTSQTESRNDLFLKTDLLKAIANSQEEVSEAMAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPFKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQEQLGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFTPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	XM_031906834.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906834.1

ORF Insert Sequence:

ATGGAAATGC GTGTCGATAC AAGCCAGACG GAGTCCAGGA ACGACTTATT CCTGAAGACG 
GACTTGTTGA AGGCGATAGC CAACAGCCAA GAGGAAGTAT CTGAAGCCAT GGCCGGCGAC 
GAAAACTTGT CGGACAAGCA GGTCGGTGAA ACGAATGGAT CTTGTGAAAA GAACGAGTCC 
AAGGAGCTCG GTTTATTGAC GCTTGCCACC CCAGACAGCA CAAAACTCCC TCACCAGGAC 
TGTGTTTTTA AAACTTTTCG TGTGGCAGAG AACGGGGTGT ACGACCGAGA CACGGAGGGG 
CAGTACCCCT TTAAAGCGAG CAACCCCACA CAGACATCCG TAACGGGAAG TAATGGGTAC 
ATTTTAAACA AACCTGCCAT TTTGGAGCAG CCATTAAGGA CTTCAAGCTT GTCACCTCTG 
AGTGGCCATG CTGCTAAAAC TCTACCGGTT GCTGCCAGCA AACCCAAAGC AGCTTCTAAT 
GTCTACGTCC CTCCTGCCCC TTCTCCTGTC CCTCCGAACC CTGGGGATCC GGGGCGGGTT 
GAGAACACAA AGCAACAGGA CAGCGCTTTA CCTGATCTGA AAGTTCACCG TGCAAGGAAA 
ACTCTACCGA AGACAAGCGG CAGTTCGCCC ATCCATAAAG AGCCCAAAGA ACCATCCGAG 
CAGGACGAAA CCAAGGACGA GAGTGCACCC AAAAATGGTT TAGATGACGA GAAAGAGGAA 
CCCCAGTCTC CCTCCTCTGC CCCTCAACAG CTGCTACCTC AGGGTCAAAA CTCTGCCAAG 
TCGCTCTCAG CTGCACTGGT GTGTCGGAAG AAGAAACGGC GGATGGGCAT GTACAGCCTC 
GTACCCAAGA AGAAGACCAA AGTGCCAAAG CAAAGAAGCG TGCTGGAGAT GTTCCGGAAC 
ATCACCTACA CCTCTTCCAG TCCCAAGAGC GAGAAAGAGT CCGATGTTCC TCATGTTAAC 
GGGGAAAGCA TGGAGATGGA TTCCGAAGAC GAGGATTCGG AAGACATGGA AGAAGAGGAC 
TACCACAGTT CGGAGACGCC AGCCTCGGAG AGTCAGGAAG AGCCCGGTGT TCCTAAAGAG 
GCCACCTCAG AAGCCGACAG TTCCAGAAAG GTCGATGAGG TTGATTCTGA AGGAGATGAA 
GACGTGGAGT CTGGGGATTC TGTAGAGGAG GAAGATGACG GAGCAGAGTC TGATTTAAGC 
TCAGAGTCCA GTTTAAGGAA GAAGCAAGAG AAGAAGAAGA CCCTGGCCGA AAGCCCCTGG 
ATGAAGGGTT CTAGGCGACG GAAGAGAAAG AACAAGGAAA ATCCCAATCC GCTTCTAGCT 
TCATCGGGAG AGGCTCGGGA AGCCAGCATG GAATACACAG AGGTCTCATT AGACTCCTTG 
GATCTGCGCG TCAAAGAGAT GTTATCTCCG CAGTCTAACG ATCTCTCTAA TGGCTCGGAG 
GCCATGGAAC TGGACGGCCT TCAGGGGATC CCCCTCTGCA GCTGCCGGAT GGAGACCCCC 
AAAGAAGAGG AGGTGTCGGC AAAGGCTGGA GAGCATTGCA TGGCCATTGA GAGCACTGGC 
CAAGAGCAGC TGGGTCGCTG TACCAACAGG GTGGCAAAGT ACGAGCTAAT GAGGCCTTCT 
AACAAGGTGC GTCATCTGGT GCTGTGTGAG GATCACCGGG GCAAGATGGT CAAACACCAG 
TGTTGTCCTG GCTGCGGTTA CTTCTGCACA GCGGGGACCT TCATGGAATG CCAGCCGGAC 
AGCACCATCT CCCACCGATT CCACAAGGAC TGCGCCACCC GGGTCAACAA CTCGAGCTAC 
TGCCCACACT GCGGGGAGGA GGCCTCCAAA GCGAAGGAGG TGACCATCGC CAAGGCCGAC 
ACCACGTCCT CCCTGGCCCC AGTGACCGGC GAATGGGAGA AAGTCAGTGC GGCAGAGGGA 
CAGGCGGACA CTACAACCGA GAGCGAGGCC ATGGCTCTAA AGGCGCCAGA GGAGAATAAG 
CAAGAAGCAA CATCTTCCCT CCCTGGAGGA ACCCCTGAGC CTATAGAGAC CCCAAGTTTG 
GGCAAACCAC CCTCTCTGTC TCAGGGGCCC GTCAAGGAGA CCTTGGAAAG CGCTCTACTC 
GCTTTGGACT CTGAAAAACC CAAGAAATTG CGGTTTCACC CAAAGCAGCT GTATTTTTCT 
GCCCGGCAAG GAGAGCTGCA GAAAGTCCTG ATGATGCTGG TGGACGGTAT TGACCCCAAC 
TACAAAATGG AGCAGCACAG TCGGAGAACA CCACTTCACG CAGCGGCAGA GATGGGTTAC 
ACGGACATCT GCCACATGCT TATACAGGCC GGAGCCAACC TAGACAGCTG CTCGGAGGAT 
CAACGCACCC CCCTGATGGA GGCAGCTGAA AATAATCATC TTGATACTGT GAAATACCTG 
GTGAAGGGCG GAGCTCTAAT TGACCCTAAG GACTCCGAGG GCTCCACTTG TCTTCATCTG 
GCGGCCAAGA AGGGTCACTA CGAAGTGGTG AAATATCTCC TCTCCTCCGA GAGAACCGAC 
GTCAACTGCC AGGACGACGG GGGCTGGACC CCGATGATCT GGGCTACCGA GTACAAGCAC 
GTGGAGATAG TCAAACTGTT ACATTCCTCC CGCGCGGACG TCAATATCCG CGATAATGAG 
GAGAACATCT GCCTGCACTG GGCCGCCTTC GCCGGTTCCG TCGAGATAGC GGAAATCCTC 
CTGGCTTCCA AATGTGACCT CCGAGCTGTG AATATCCACG GGGACTCCCC GCTCCATATT 
GCCGCCCGCG AGAACCGCCA CGAGTGCGTC ATGCTGTTCC TGGGTCACGG CTCGGAAGTG 
GGACTGAAGA ACAAAGAGGG CGAGACGCCG CTGGAGTGCT CGGCGCCCAA CTCCGACGTT 
TGGGCGGCCC TTAGCAAGTG CCAGGCCTTG CCCGAGAAGC CCACGCTGCA GGAAAAGGTG 
GTGGACAGGG ATATATCCCG GGGGTACGAG AATATCCCCA TCCCTTGCGT CAACGCCGAA 
GACTCCGAGC TGTGTCCCGT CAACTACAAA TACGTCTCTC AGAACTGCGT GACCTCCCCG 
CTGAACATCG ACAGAAACAT TACCCATCTG CAGTACTGTG TGTGCATCGA TGACTGTTCC 
TCTAGTAACT GTATGTGCGG CCAGCTGAGT ATGAGGTGCT GGTACGACAA GAACGGGCGC 
TTGTTACCAG AGTTCAACAT GTTGGAGCCC CCACTGATCT TCGAATGCAA CCACGCCTGC 
TCCTGCTGGC GCAACTGCCG CAACCGTGTG GTTCAAAATG GCTTGAAGAT TAGATTGCAA 
CTGTTCCGCA CCAAAAGCAA AGGCTGGGGG GTGCGATCCC TGCAAGACAT TCCCCAGGGG 
ACCTTCGTAT GCGAGTATGT GGGGGAGCTG ATTTCGGACG CAGAGGCTGA CGTTCGAGAG 
GATGACACCT ACCTCTTTGA TCTTGATAAC AAGGACCGAG AGGTTTACTG CATCGACGCC 
CGTTTCTACG GCAACATCAG TCGCTTTATC AACCACCTCT GCGAGCCGAA CCTGCTCCCC 
GTGCGAGTCT TTATGTCCCA CCAGGATCTG CGCTTTCCCC GCATCGGCTT CTTCAGCAGC 
CGGCACATCG GCGCGGGGGA GGAGATCGGG TTTGATTACG GCGACCGATT CTGGGACGTC 
AAGGGGAAGC TCTTCAGCTG CCAGTGCGGT TCCCCCAAGT GCAAGCACCC AATCAAAGTC 
CTTTCCCCGA GCCAGTCATT CACCCCCGCA GAAACTCCGT CGGAGATTGG CTTACCCGAC 
ACCAGCTCGG CCACCCCCCC GAGCCCCTCG CAGCCGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1 ( XM_031906837.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762697.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa63381
Clone ID Related Accession (Same CDS sequence)	XM_031906837.1
Accession Version	XM_031906837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTCG ATACAAGCCA GACGGAGTCC AGGAACGACT TATTCCTGAA GACGGACTTG 
TTGAAGGCGA TAGCCAACAG CCAAGAGGAA GTATCTGAAG CCATGGCCGG CGACGAAAAC 
TTGTCGGACA AGCAGGTCGG TGAAACGAAT GGATCTTGTG AAAAGAACGA GTCCAAGGAG 
CTCGGTTTAT TGACGCTTGC CACCCCAGAC AGCACAAAAC TCCCTCACCA GGACTGTGTT 
TTTAAAACTT TTCGTGTGGC AGAGAACGGG GTGTACGACC GAGACACGGA GGGGCAGTAC 
CCCTTTAAAG CGAGCAACCC CACACAGACA TCCGTAACGG GAAGTAATGG GTACATTTTA 
AACAAACCTG CCATTTTGGA GCAGCCATTA AGGACTTCAA GCTTGTCACC TCTGAGTGGC 
CATGCTGCTA AAACTCTACC GGTTGCTGCC AGCAAACCCA AAGCAGCTTC TAATGTCTAC 
GTCCCTCCTG CCCCTTCTCC TGTCCCTCCG AACCCTGGGG ATCCGGGGCG GGTTGAGAAC 
ACAAAGCAAC AGGACAGCGC TTTACCTGAT CTGAAAGTTC ACCGTGCAAG GAAAACTCTA 
CCGAAGACAA GCGGCAGTTC GCCCATCCAT AAAGAGCCCA AAGAACCATC CGAGCAGGAC 
GAAACCAAGG ACGAGAGTGC ACCCAAAAAT GGTTTAGATG ACGAGAAAGA GGAACCCCAG 
TCTCCCTCCT CTGCCCCTCA ACAGCTGCTA CCTCAGGGTC AAAACTCTGC CAAGTCGCTC 
TCAGCTGCAC TGGTGTGTCG GAAGAAGAAA CGGCGGATGG GCATGTACAG CCTCGTACCC 
AAGAAGAAGA CCAAAGTGCC AAAGCAAAGA AGCGTGCTGG AGATGTTCCG GAACATCACC 
TACACCTCTT CCAGTCCCAA GAGCGAGAAA GAGTCCGATG TTCCTCATGT TAACGGGGAA 
AGCATGGAGA TGGATTCCGA AGACGAGGAT TCGGAAGACA TGGAAGAAGA GGACTACCAC 
AGTTCGGAGA CGCCAGCCTC GGAGAGTCAG GAAGAGCCCG GTGTTCCTAA AGAGGCCACC 
TCAGAAGCCG ACAGTTCCAG AAAGGTCGAT GAGGTTGATT CTGAAGGAGA TGAAGACGTG 
GAGTCTGGGG ATTCTGTAGA GGAGGAAGAT GACGGAGCAG AGTCTGATTT AAGCTCAGAG 
TCCAGTTTAA GGAAGAAGCA AGAGAAGAAG AAGACCCTGG CCGAAAGCCC CTGGATGAAG 
GGTTCTAGGC GACGGAAGAG AAAGAACAAG GAAAATCCCA ATCCGCTTCT AGCTTCATCG 
GGAGAGGCTC GGGAAGCCAG CATGGAATAC ACAGAGGTCT CATTAGACTC CTTGGATCTG 
CGCGTCAAAG AGATGTTATC TCCGCAGTCT AACGATCTCT CTAATGGCTC GGAGGCCATG 
GAACTGGACG GCCTTCAGGG GATCCCCCTC TGCAGCTGCC GGATGGAGAC CCCCAAAGAA 
GAGGAGGTGT CGGCAAAGGC TGGAGAGCAT TGCATGGCCA TTGAGAGCAC TGGCCAAGAG 
CAGCTGGGTC GCTGTACCAA CAGGGTGGCA AAGTACGAGC TAATGAGGCC TTCTAACAAG 
GTGCGTCATC TGGTGCTGTG TGAGGATCAC CGGGGCAAGA TGGTCAAACA CCAGTGTTGT 
CCTGGCTGCG GTTACTTCTG CACAGCGGGG ACCTTCATGG AATGCCAGCC GGACAGCACC 
ATCTCCCACC GATTCCACAA GGACTGCGCC ACCCGGGTCA ACAACTCGAG CTACTGCCCA 
CACTGCGGGG AGGAGGCCTC CAAAGCGAAG GAGGTGACCA TCGCCAAGGC CGACACCACG 
TCCTCCCTGG CCCCAGTGAC CGGCGAATGG GAGAAAGTCA GTGCGGCAGA GGGACAGGCG 
GACACTACAA CCGAGAGCGA GGCCATGGCT CTAAAGGCGC CAGAGGAGAA TAAGCAAGAA 
GCAACATCTT CCCTCCCTGG AGGAACCCCT GAGCCTATAG AGACCCCAAG TTTGGGCAAA 
CCACCCTCTC TGTCTCAGGG GCCCGTCAAG GAGACCTTGG AAAGCGCTCT ACTCGCTTTG 
GACTCTGAAA AACCCAAGAA ATTGCGGTTT CACCCAAAGC AGCTGTATTT TTCTGCCCGG 
CAAGGAGAGC TGCAGAAAGT CCTGATGATG CTGGTGGACG GTATTGACCC CAACTACAAA 
ATGGAGCAGC ACAGTCGGAG AACACCACTT CACGCAGCGG CAGAGATGGG TTACACGGAC 
ATCTGCCACA TGCTTATACA GGCCGGAGCC AACCTAGACA GCTGCTCGGA GGATCAACGC 
ACCCCCCTGA TGGAGGCAGC TGAAAATAAT CATCTTGATA CTGTGAAATA CCTGGTGAAG 
GGCGGAGCTC TAATTGACCC TAAGGACTCC GAGGGCTCCA CTTGTCTTCA TCTGGCGGCC 
AAGAAGGGTC ACTACGAAGT GGTGAAATAT CTCCTCTCCT CCGAGAGAAC CGACGTCAAC 
TGCCAGGACG ACGGGGGCTG GACCCCGATG ATCTGGGCTA CCGAGTACAA GCACGTGGAG 
ATAGTCAAAC TGTTACATTC CTCCCGCGCG GACGTCAATA TCCGCGATAA TGAGGAGAAC 
ATCTGCCTGC ACTGGGCCGC CTTCGCCGGT TCCGTCGAGA TAGCGGAAAT CCTCCTGGCT 
TCCAAATGTG ACCTCCGAGC TGTGAATATC CACGGGGACT CCCCGCTCCA TATTGCCGCC 
CGCGAGAACC GCCACGAGTG CGTCATGCTG TTCCTGGGTC ACGGCTCGGA AGTGGGACTG 
AAGAACAAAG AGGGCGAGAC GCCGCTGGAG TGCTCGGCGC CCAACTCCGA CGTTTGGGCG 
GCCCTTAGCA AGTGCCAGGC CTTGCCCGAG AAGCCCACGC TGCAGGAAAA GGTGGTGGAC 
AGGGATATAT CCCGGGGGTA CGAGAATATC CCCATCCCTT GCGTCAACGC CGAAGACTCC 
GAGCTGTGTC CCGTCAACTA CAAATACGTC TCTCAGAACT GCGTGACCTC CCCGCTGAAC 
ATCGACAGAA ACATTACCCA TCTGCAGTAC TGTGTGTGCA TCGATGACTG TTCCTCTAGT 
AACTGTATGT GCGGCCAGCT GAGTATGAGG TGCTGGTACG ACAAGAACGG GCGCTTGTTA 
CCAGAGTTCA ACATGTTGGA GCCCCCACTG ATCTTCGAAT GCAACCACGC CTGCTCCTGC 
TGGCGCAACT GCCGCAACCG TGTGGTTCAA AATGGCTTGA AGATTAGATT GCAACTGTTC 
CGCACCAAAA GCAAAGGCTG GGGGGTGCGA TCCCTGCAAG ACATTCCCCA GGGGACCTTC 
GTATGCGAGT ATGTGGGGGA GCTGATTTCG GACGCAGAGG CTGACGTTCG AGAGGATGAC 
ACCTACCTCT TTGATCTTGA TAACAAGGAC CGAGAGGTTT ACTGCATCGA CGCCCGTTTC 
TACGGCAACA TCAGTCGCTT TATCAACCAC CTCTGCGAGC CGAACCTGCT CCCCGTGCGA 
GTCTTTATGT CCCACCAGGA TCTGCGCTTT CCCCGCATCG GCTTCTTCAG CAGCCGGCAC 
ATCGGCGCGG GGGAGGAGAT CGGGTTTGAT TACGGCGACC GATTCTGGGA CGTCAAGGGG 
AAGCTCTTCA GCTGCCAGTG CGGTTCCCCC AAGTGCAAGC ACCCAATCAA AGTCCTTTCC 
CCGAGCCAGT CATTCACCCC CGCAGAAACT CCGTCGGAGA TTGGCTTACC CGACACCAGC 
TCGGCCACCC CCCCGAGCCC CTCGCAGCCG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762697.1
CDS	88..3903
Translation	MRVDTSQTESRNDLFLKTDLLKAIANSQEEVSEAMAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPFKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQEQLGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFTPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	XM_031906837.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906837.1

ORF Insert Sequence:

ATGCGTGTCG ATACAAGCCA GACGGAGTCC AGGAACGACT TATTCCTGAA GACGGACTTG 
TTGAAGGCGA TAGCCAACAG CCAAGAGGAA GTATCTGAAG CCATGGCCGG CGACGAAAAC 
TTGTCGGACA AGCAGGTCGG TGAAACGAAT GGATCTTGTG AAAAGAACGA GTCCAAGGAG 
CTCGGTTTAT TGACGCTTGC CACCCCAGAC AGCACAAAAC TCCCTCACCA GGACTGTGTT 
TTTAAAACTT TTCGTGTGGC AGAGAACGGG GTGTACGACC GAGACACGGA GGGGCAGTAC 
CCCTTTAAAG CGAGCAACCC CACACAGACA TCCGTAACGG GAAGTAATGG GTACATTTTA 
AACAAACCTG CCATTTTGGA GCAGCCATTA AGGACTTCAA GCTTGTCACC TCTGAGTGGC 
CATGCTGCTA AAACTCTACC GGTTGCTGCC AGCAAACCCA AAGCAGCTTC TAATGTCTAC 
GTCCCTCCTG CCCCTTCTCC TGTCCCTCCG AACCCTGGGG ATCCGGGGCG GGTTGAGAAC 
ACAAAGCAAC AGGACAGCGC TTTACCTGAT CTGAAAGTTC ACCGTGCAAG GAAAACTCTA 
CCGAAGACAA GCGGCAGTTC GCCCATCCAT AAAGAGCCCA AAGAACCATC CGAGCAGGAC 
GAAACCAAGG ACGAGAGTGC ACCCAAAAAT GGTTTAGATG ACGAGAAAGA GGAACCCCAG 
TCTCCCTCCT CTGCCCCTCA ACAGCTGCTA CCTCAGGGTC AAAACTCTGC CAAGTCGCTC 
TCAGCTGCAC TGGTGTGTCG GAAGAAGAAA CGGCGGATGG GCATGTACAG CCTCGTACCC 
AAGAAGAAGA CCAAAGTGCC AAAGCAAAGA AGCGTGCTGG AGATGTTCCG GAACATCACC 
TACACCTCTT CCAGTCCCAA GAGCGAGAAA GAGTCCGATG TTCCTCATGT TAACGGGGAA 
AGCATGGAGA TGGATTCCGA AGACGAGGAT TCGGAAGACA TGGAAGAAGA GGACTACCAC 
AGTTCGGAGA CGCCAGCCTC GGAGAGTCAG GAAGAGCCCG GTGTTCCTAA AGAGGCCACC 
TCAGAAGCCG ACAGTTCCAG AAAGGTCGAT GAGGTTGATT CTGAAGGAGA TGAAGACGTG 
GAGTCTGGGG ATTCTGTAGA GGAGGAAGAT GACGGAGCAG AGTCTGATTT AAGCTCAGAG 
TCCAGTTTAA GGAAGAAGCA AGAGAAGAAG AAGACCCTGG CCGAAAGCCC CTGGATGAAG 
GGTTCTAGGC GACGGAAGAG AAAGAACAAG GAAAATCCCA ATCCGCTTCT AGCTTCATCG 
GGAGAGGCTC GGGAAGCCAG CATGGAATAC ACAGAGGTCT CATTAGACTC CTTGGATCTG 
CGCGTCAAAG AGATGTTATC TCCGCAGTCT AACGATCTCT CTAATGGCTC GGAGGCCATG 
GAACTGGACG GCCTTCAGGG GATCCCCCTC TGCAGCTGCC GGATGGAGAC CCCCAAAGAA 
GAGGAGGTGT CGGCAAAGGC TGGAGAGCAT TGCATGGCCA TTGAGAGCAC TGGCCAAGAG 
CAGCTGGGTC GCTGTACCAA CAGGGTGGCA AAGTACGAGC TAATGAGGCC TTCTAACAAG 
GTGCGTCATC TGGTGCTGTG TGAGGATCAC CGGGGCAAGA TGGTCAAACA CCAGTGTTGT 
CCTGGCTGCG GTTACTTCTG CACAGCGGGG ACCTTCATGG AATGCCAGCC GGACAGCACC 
ATCTCCCACC GATTCCACAA GGACTGCGCC ACCCGGGTCA ACAACTCGAG CTACTGCCCA 
CACTGCGGGG AGGAGGCCTC CAAAGCGAAG GAGGTGACCA TCGCCAAGGC CGACACCACG 
TCCTCCCTGG CCCCAGTGAC CGGCGAATGG GAGAAAGTCA GTGCGGCAGA GGGACAGGCG 
GACACTACAA CCGAGAGCGA GGCCATGGCT CTAAAGGCGC CAGAGGAGAA TAAGCAAGAA 
GCAACATCTT CCCTCCCTGG AGGAACCCCT GAGCCTATAG AGACCCCAAG TTTGGGCAAA 
CCACCCTCTC TGTCTCAGGG GCCCGTCAAG GAGACCTTGG AAAGCGCTCT ACTCGCTTTG 
GACTCTGAAA AACCCAAGAA ATTGCGGTTT CACCCAAAGC AGCTGTATTT TTCTGCCCGG 
CAAGGAGAGC TGCAGAAAGT CCTGATGATG CTGGTGGACG GTATTGACCC CAACTACAAA 
ATGGAGCAGC ACAGTCGGAG AACACCACTT CACGCAGCGG CAGAGATGGG TTACACGGAC 
ATCTGCCACA TGCTTATACA GGCCGGAGCC AACCTAGACA GCTGCTCGGA GGATCAACGC 
ACCCCCCTGA TGGAGGCAGC TGAAAATAAT CATCTTGATA CTGTGAAATA CCTGGTGAAG 
GGCGGAGCTC TAATTGACCC TAAGGACTCC GAGGGCTCCA CTTGTCTTCA TCTGGCGGCC 
AAGAAGGGTC ACTACGAAGT GGTGAAATAT CTCCTCTCCT CCGAGAGAAC CGACGTCAAC 
TGCCAGGACG ACGGGGGCTG GACCCCGATG ATCTGGGCTA CCGAGTACAA GCACGTGGAG 
ATAGTCAAAC TGTTACATTC CTCCCGCGCG GACGTCAATA TCCGCGATAA TGAGGAGAAC 
ATCTGCCTGC ACTGGGCCGC CTTCGCCGGT TCCGTCGAGA TAGCGGAAAT CCTCCTGGCT 
TCCAAATGTG ACCTCCGAGC TGTGAATATC CACGGGGACT CCCCGCTCCA TATTGCCGCC 
CGCGAGAACC GCCACGAGTG CGTCATGCTG TTCCTGGGTC ACGGCTCGGA AGTGGGACTG 
AAGAACAAAG AGGGCGAGAC GCCGCTGGAG TGCTCGGCGC CCAACTCCGA CGTTTGGGCG 
GCCCTTAGCA AGTGCCAGGC CTTGCCCGAG AAGCCCACGC TGCAGGAAAA GGTGGTGGAC 
AGGGATATAT CCCGGGGGTA CGAGAATATC CCCATCCCTT GCGTCAACGC CGAAGACTCC 
GAGCTGTGTC CCGTCAACTA CAAATACGTC TCTCAGAACT GCGTGACCTC CCCGCTGAAC 
ATCGACAGAA ACATTACCCA TCTGCAGTAC TGTGTGTGCA TCGATGACTG TTCCTCTAGT 
AACTGTATGT GCGGCCAGCT GAGTATGAGG TGCTGGTACG ACAAGAACGG GCGCTTGTTA 
CCAGAGTTCA ACATGTTGGA GCCCCCACTG ATCTTCGAAT GCAACCACGC CTGCTCCTGC 
TGGCGCAACT GCCGCAACCG TGTGGTTCAA AATGGCTTGA AGATTAGATT GCAACTGTTC 
CGCACCAAAA GCAAAGGCTG GGGGGTGCGA TCCCTGCAAG ACATTCCCCA GGGGACCTTC 
GTATGCGAGT ATGTGGGGGA GCTGATTTCG GACGCAGAGG CTGACGTTCG AGAGGATGAC 
ACCTACCTCT TTGATCTTGA TAACAAGGAC CGAGAGGTTT ACTGCATCGA CGCCCGTTTC 
TACGGCAACA TCAGTCGCTT TATCAACCAC CTCTGCGAGC CGAACCTGCT CCCCGTGCGA 
GTCTTTATGT CCCACCAGGA TCTGCGCTTT CCCCGCATCG GCTTCTTCAG CAGCCGGCAC 
ATCGGCGCGG GGGAGGAGAT CGGGTTTGAT TACGGCGACC GATTCTGGGA CGTCAAGGGG 
AAGCTCTTCA GCTGCCAGTG CGGTTCCCCC AAGTGCAAGC ACCCAATCAA AGTCCTTTCC 
CCGAGCCAGT CATTCACCCC CGCAGAAACT CCGTCGGAGA TTGGCTTACC CGACACCAGC 
TCGGCCACCC CCCCGAGCCC CTCGCAGCCG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1 ( XM_031906838.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762698.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa63382
Clone ID Related Accession (Same CDS sequence)	XM_031906838.1
Accession Version	XM_031906838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCA GCTGGGTCGC TGTACCAACA GGGTGGCAAA GTACGAGCTA 
ATGAGGCCTT CTAACAAGGT GCGTCATCTG GTGCTGTGTG AGGATCACCG GGGCAAGATG 
GTCAAACACC AGTGTTGTCC TGGCTGCGGT TACTTCTGCA CAGCGGGGAC CTTCATGGAA 
TGCCAGCCGG ACAGCACCAT CTCCCACCGA TTCCACAAGG ACTGCGCCAC CCGGGTCAAC 
AACTCGAGCT ACTGCCCACA CTGCGGGGAG GAGGCCTCCA AAGCGAAGGA GGTGACCATC 
GCCAAGGCCG ACACCACGTC CTCCCTGGCC CCAGTGACCG GCGAATGGGA GAAAGTCAGT 
GCGGCAGAGG GACAGGCGGA CACTACAACC GAGAGCGAGG CCATGGCTCT AAAGGCGCCA 
GAGGAGAATA AGCAAGAAGC AACATCTTCC CTCCCTGGAG GAACCCCTGA GCCTATAGAG 
ACCCCAAGTT TGGGCAAACC ACCCTCTCTG TCTCAGGGGC CCGTCAAGGA GACCTTGGAA 
AGCGCTCTAC TCGCTTTGGA CTCTGAAAAA CCCAAGAAAT TGCGGTTTCA CCCAAAGCAG 
CTGTATTTTT CTGCCCGGCA AGGAGAGCTG CAGAAAGTCC TGATGATGCT GGTGGACGGT 
ATTGACCCCA ACTACAAAAT GGAGCAGCAC AGTCGGAGAA CACCACTTCA CGCAGCGGCA 
GAGATGGGTT ACACGGACAT CTGCCACATG CTTATACAGG CCGGAGCCAA CCTAGACAGC 
TGCTCGGAGG ATCAACGCAC CCCCCTGATG GAGGCAGCTG AAAATAATCA TCTTGATACT 
GTGAAATACC TGGTGAAGGG CGGAGCTCTA ATTGACCCTA AGGACTCCGA GGGCTCCACT 
TGTCTTCATC TGGCGGCCAA GAAGGGTCAC TACGAAGTGG TGAAATATCT CCTCTCCTCC 
GAGAGAACCG ACGTCAACTG CCAGGACGAC GGGGGCTGGA CCCCGATGAT CTGGGCTACC 
GAGTACAAGC ACGTGGAGAT AGTCAAACTG TTACATTCCT CCCGCGCGGA CGTCAATATC 
CGCGATAATG AGGAGAACAT CTGCCTGCAC TGGGCCGCCT TCGCCGGTTC CGTCGAGATA 
GCGGAAATCC TCCTGGCTTC CAAATGTGAC CTCCGAGCTG TGAATATCCA CGGGGACTCC 
CCGCTCCATA TTGCCGCCCG CGAGAACCGC CACGAGTGCG TCATGCTGTT CCTGGGTCAC 
GGCTCGGAAG TGGGACTGAA GAACAAAGAG GGCGAGACGC CGCTGGAGTG CTCGGCGCCC 
AACTCCGACG TTTGGGCGGC CCTTAGCAAG TGCCAGGCCT TGCCCGAGAA GCCCACGCTG 
CAGGAAAAGG TGGTGGACAG GGATATATCC CGGGGGTACG AGAATATCCC CATCCCTTGC 
GTCAACGCCG AAGACTCCGA GCTGTGTCCC GTCAACTACA AATACGTCTC TCAGAACTGC 
GTGACCTCCC CGCTGAACAT CGACAGAAAC ATTACCCATC TGCAGTACTG TGTGTGCATC 
GATGACTGTT CCTCTAGTAA CTGTATGTGC GGCCAGCTGA GTATGAGGTG CTGGTACGAC 
AAGAACGGGC GCTTGTTACC AGAGTTCAAC ATGTTGGAGC CCCCACTGAT CTTCGAATGC 
AACCACGCCT GCTCCTGCTG GCGCAACTGC CGCAACCGTG TGGTTCAAAA TGGCTTGAAG 
ATTAGATTGC AACTGTTCCG CACCAAAAGC AAAGGCTGGG GGGTGCGATC CCTGCAAGAC 
ATTCCCCAGG GGACCTTCGT ATGCGAGTAT GTGGGGGAGC TGATTTCGGA CGCAGAGGCT 
GACGTTCGAG AGGATGACAC CTACCTCTTT GATCTTGATA ACAAGGACCG AGAGGTTTAC 
TGCATCGACG CCCGTTTCTA CGGCAACATC AGTCGCTTTA TCAACCACCT CTGCGAGCCG 
AACCTGCTCC CCGTGCGAGT CTTTATGTCC CACCAGGATC TGCGCTTTCC CCGCATCGGC 
TTCTTCAGCA GCCGGCACAT CGGCGCGGGG GAGGAGATCG GGTTTGATTA CGGCGACCGA 
TTCTGGGACG TCAAGGGGAA GCTCTTCAGC TGCCAGTGCG GTTCCCCCAA GTGCAAGCAC 
CCAATCAAAG TCCTTTCCCC GAGCCAGTCA TTCACCCCCG CAGAAACTCC GTCGGAGATT 
GGCTTACCCG ACACCAGCTC GGCCACCCCC CCGAGCCCCT CGCAGCCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762698.1
CDS	329..4042
Translation	MAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPFKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQEQLGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFTPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	XM_031906838.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906838.1

ORF Insert Sequence:

ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTTAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGATGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCTG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCA GCTGGGTCGC TGTACCAACA GGGTGGCAAA GTACGAGCTA 
ATGAGGCCTT CTAACAAGGT GCGTCATCTG GTGCTGTGTG AGGATCACCG GGGCAAGATG 
GTCAAACACC AGTGTTGTCC TGGCTGCGGT TACTTCTGCA CAGCGGGGAC CTTCATGGAA 
TGCCAGCCGG ACAGCACCAT CTCCCACCGA TTCCACAAGG ACTGCGCCAC CCGGGTCAAC 
AACTCGAGCT ACTGCCCACA CTGCGGGGAG GAGGCCTCCA AAGCGAAGGA GGTGACCATC 
GCCAAGGCCG ACACCACGTC CTCCCTGGCC CCAGTGACCG GCGAATGGGA GAAAGTCAGT 
GCGGCAGAGG GACAGGCGGA CACTACAACC GAGAGCGAGG CCATGGCTCT AAAGGCGCCA 
GAGGAGAATA AGCAAGAAGC AACATCTTCC CTCCCTGGAG GAACCCCTGA GCCTATAGAG 
ACCCCAAGTT TGGGCAAACC ACCCTCTCTG TCTCAGGGGC CCGTCAAGGA GACCTTGGAA 
AGCGCTCTAC TCGCTTTGGA CTCTGAAAAA CCCAAGAAAT TGCGGTTTCA CCCAAAGCAG 
CTGTATTTTT CTGCCCGGCA AGGAGAGCTG CAGAAAGTCC TGATGATGCT GGTGGACGGT 
ATTGACCCCA ACTACAAAAT GGAGCAGCAC AGTCGGAGAA CACCACTTCA CGCAGCGGCA 
GAGATGGGTT ACACGGACAT CTGCCACATG CTTATACAGG CCGGAGCCAA CCTAGACAGC 
TGCTCGGAGG ATCAACGCAC CCCCCTGATG GAGGCAGCTG AAAATAATCA TCTTGATACT 
GTGAAATACC TGGTGAAGGG CGGAGCTCTA ATTGACCCTA AGGACTCCGA GGGCTCCACT 
TGTCTTCATC TGGCGGCCAA GAAGGGTCAC TACGAAGTGG TGAAATATCT CCTCTCCTCC 
GAGAGAACCG ACGTCAACTG CCAGGACGAC GGGGGCTGGA CCCCGATGAT CTGGGCTACC 
GAGTACAAGC ACGTGGAGAT AGTCAAACTG TTACATTCCT CCCGCGCGGA CGTCAATATC 
CGCGATAATG AGGAGAACAT CTGCCTGCAC TGGGCCGCCT TCGCCGGTTC CGTCGAGATA 
GCGGAAATCC TCCTGGCTTC CAAATGTGAC CTCCGAGCTG TGAATATCCA CGGGGACTCC 
CCGCTCCATA TTGCCGCCCG CGAGAACCGC CACGAGTGCG TCATGCTGTT CCTGGGTCAC 
GGCTCGGAAG TGGGACTGAA GAACAAAGAG GGCGAGACGC CGCTGGAGTG CTCGGCGCCC 
AACTCCGACG TTTGGGCGGC CCTTAGCAAG TGCCAGGCCT TGCCCGAGAA GCCCACGCTG 
CAGGAAAAGG TGGTGGACAG GGATATATCC CGGGGGTACG AGAATATCCC CATCCCTTGC 
GTCAACGCCG AAGACTCCGA GCTGTGTCCC GTCAACTACA AATACGTCTC TCAGAACTGC 
GTGACCTCCC CGCTGAACAT CGACAGAAAC ATTACCCATC TGCAGTACTG TGTGTGCATC 
GATGACTGTT CCTCTAGTAA CTGTATGTGC GGCCAGCTGA GTATGAGGTG CTGGTACGAC 
AAGAACGGGC GCTTGTTACC AGAGTTCAAC ATGTTGGAGC CCCCACTGAT CTTCGAATGC 
AACCACGCCT GCTCCTGCTG GCGCAACTGC CGCAACCGTG TGGTTCAAAA TGGCTTGAAG 
ATTAGATTGC AACTGTTCCG CACCAAAAGC AAAGGCTGGG GGGTGCGATC CCTGCAAGAC 
ATTCCCCAGG GGACCTTCGT ATGCGAGTAT GTGGGGGAGC TGATTTCGGA CGCAGAGGCT 
GACGTTCGAG AGGATGACAC CTACCTCTTT GATCTTGATA ACAAGGACCG AGAGGTTTAC 
TGCATCGACG CCCGTTTCTA CGGCAACATC AGTCGCTTTA TCAACCACCT CTGCGAGCCG 
AACCTGCTCC CCGTGCGAGT CTTTATGTCC CACCAGGATC TGCGCTTTCC CCGCATCGGC 
TTCTTCAGCA GCCGGCACAT CGGCGCGGGG GAGGAGATCG GGTTTGATTA CGGCGACCGA 
TTCTGGGACG TCAAGGGGAA GCTCTTCAGC TGCCAGTGCG GTTCCCCCAA GTGCAAGCAC 
CCAATCAAAG TCCTTTCCCC GAGCCAGTCA TTCACCCCCG CAGAAACTCC GTCGGAGATT 
GGCTTACCCG ACACCAGCTC GGCCACCCCC CCGAGCCCCT CGCAGCCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1 ( NM_001102698.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096168.1 Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), mRNA.

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CloneID	OXa01844
Clone ID Related Accession (Same CDS sequence)	NM_001102698.1
Accession Version	NM_001102698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase EHMT1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC135982.1. ##Evidence-Data-START## Transcript exon combination :: BC135982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028302 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTAAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGACGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCCG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCT GGGTCGCTGT ACCAACAGGG TGGCAAAGTA CGAGCTAATG 
AGGCCTTCTA ACAAGGTGCG TCATCTGGTG CTGTGTGAGG ATCACCGGGG CAAGATGGTC 
AAACACCAGT GTTGTCCTGG CTGCGGTTAC TTCTGCACAG CGGGGACCTT CATGGAATGC 
CAGCCGGACA GCACCATCTC CCACCGATTC CACAAGGACT GCGCCACCCG GGTCAACAAC 
TCGAGCTACT GCCCACACTG CGGGGAGGAG GCCTCCAAAG CGAAGGAGGT GACCATCGCC 
AAGGCCGACA CCACGTCCTC CCTGGCCCCA GTGACCGGCG AATGGGAGAA AGTCAGTGCG 
GCAGAGGGAC AGGCGGACAC TACAACCGAG AGCGAGGCCA TGGCTCTAAA GGCGCCAGAG 
GAGAATAAGC AAGAAGCAAC ATCTTCCCTC CCTGGAGGAA CCCCTGAGCC TATAGAGACC 
CCAAGTTTGG GCAAACCACC CTCTCTGTCT CAGGGGCCCG TCAAGGAGAC CTTGGAAAGC 
GCTCTACTCG CTTTGGACTC TGAAAAACCC AAGAAATTGC GGTTTCACCC AAAGCAGCTG 
TATTTTTCTG CCCGGCAAGG AGAGCTGCAG AAAGTCCTGA TGATGCTGGT GGACGGTATT 
GACCCCAACT ACAAAATGGA GCAGCACAGT CGGAGAACAC CACTTCACGC AGCGGCAGAG 
ATGGGTTACA CGGACATCTG CCACATGCTT ATACAGGCCG GAGCCAACCT AGACAGCTGC 
TCGGAGGATC AACGCACCCC CCTGATGGAG GCAGCTGAAA ATAATCATCT CGATACTGTG 
AAATACCTGG TGAAGGGCGG AGCTCTAATT GACCCTAAGG ACTCCGAGGG CTCCACTTGT 
CTTCATCTGG CGGCCAAGAA GGGTCACTAC GAAGTGGTGA AATATCTCCT CTCCTCCGAG 
AGAACCGACG TCAACTGCCA GGACGACGGG GGCTGGACCC CGATGATCTG GGCTACCGAG 
TACAAGCACG TGGAGATAGT CAAACTGTTA CATTCCTCCC GCGCGGACGT CAATATCCGC 
GATAATGAGG AGAACATCTG CCTGCACTGG GCCGCCTTCG CCGGTTCCGT CGAGATAGCG 
GAAATCCTCC TGGCTTCCAA ATGTGACCTC CGAGCTGTGA ATATCCACGG GGACTCCCCG 
CTCCATATTG CCGCCCGCGA GAACCGCCAC GAGTGCGTCA TGCTGTTCCT GGGTCACGGC 
TCGGAAGTGG GACTGAAGAA CAAAGAGGGC GAGACGCCGC TGGAGTGCTC GGCGCCCAAC 
TCCGACGTTT GGGCGGCCCT TAGCAAGTGC CAGGCCTTGC CCGAGAAGCC CACGCTGCAG 
GAAAAGGTGG TGGACAGGGA TATATCCCGG GGGTACGAGA ATATCCCCAT CCCTTGCGTC 
AACGCCGAAG ACTCCGAGCT GTGTCCCGTC AACTACAAAT ACGTCTCTCA GAACTGCGTG 
ACCTCCCCGC TGAACATCGA CAGAAACATT ACCCATCTGC AGTACTGTGT GTGCATCGAT 
GACTGTTCCT CTAGTAACTG TATGTGTGGC CAGCTGAGTA TGAGGTGCTG GTATGACAAG 
AACGGGCGCT TGTTACCGGA GTTCAACATG TTGGAGCCCC CGCTGATCTT CGAATGCAAC 
CACGCCTGCT CCTGCTGGCG CAACTGCCGC AACCGTGTGG TTCAAAATGG CTTGAAGATT 
AGATTGCAAC TGTTCCGCAC CAAAAGCAAA GGCTGGGGGG TGCGATCCCT GCAAGACATT 
CCCCAGGGGA CCTTCGTATG CGAGTATGTG GGGGAGCTGA TTTCGGACGC AGAGGCTGAC 
GTTCGAGAGG ATGACACCTA CCTCTTTGAT CTTGATAACA AGGACCGAGA GGTTTACTGC 
ATCGACGCCC GTTTCTACGG CAACATCAGT CGCTTTATCA ACCACCTCTG CGAGCCGAAC 
CTGCTCCCCG TGCGAGTCTT TATGTCCCAC CAGGATCTGC GCTTTCCCCG CATCGGCTTC 
TTCAGCAGCC GGCACATCGG CGCGGGGGAG GAGATCGGGT TTGATTACGG CGACCGATTC 
TGGGACGTCA AGGGGAAGCT CTTCAGCTGC CAGTGCGGTT CCCCCAAGTG CAAGCACCCA 
ATCAAAGTCC TTTCCCCGAG CCAGTCATTC GCCCCCGCAG AAACTCCGTC GGAGATTGGC 
TTACCCGACA CCAGCTCAGC CACCCCCCCG AGCCCCTCGC AGCCGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096168.1
CDS	334..4044
Translation	MAGDENLSDKQVGETNGSCEKNESKELGLLTLATPDSTKLPHQDCVFKTFRVAENGVYDRDTEGQYPLKASNPTQTSVTGSNGYILNKPAILEQPLRTSSLSPLSGHAAKTLPVAASKPKAASNVYVPPAPSPVPPNPGDPGRVENTKQQDSALPDLKVHRARKTLPKTSGSSPIHKEPKEPSEQDETKDESAPKNGLDDEKEEPQSPSSAPQQLLPQGQNSAKSLSAALVCRKKKRRMGMYSLVPKKKTKVPKQRSVLEMFRNITYTSSSPKSEKESDVPHVNGESMEMDSEDEDSEDMEEEDYHSSETPASESQEEPGVPKEATSEADSSRKVDEVDSEGDEDVESGDSVEEEDDGAESDLSSESSLRKKQEKKKTLAESPWMKGSRRRKRKNKENPNPLLASSGEAREASMEYTEVSLDSLDLRVKEMLSPQSNDLSNGSEAMELDGLQGIPLCSCRMETPKEEEVSAKAGEHCMAIESTGQELGRCTNRVAKYELMRPSNKVRHLVLCEDHRGKMVKHQCCPGCGYFCTAGTFMECQPDSTISHRFHKDCATRVNNSSYCPHCGEEASKAKEVTIAKADTTSSLAPVTGEWEKVSAAEGQADTTTESEAMALKAPEENKQEATSSLPGGTPEPIETPSLGKPPSLSQGPVKETLESALLALDSEKPKKLRFHPKQLYFSARQGELQKVLMMLVDGIDPNYKMEQHSRRTPLHAAAEMGYTDICHMLIQAGANLDSCSEDQRTPLMEAAENNHLDTVKYLVKGGALIDPKDSEGSTCLHLAAKKGHYEVVKYLLSSERTDVNCQDDGGWTPMIWATEYKHVEIVKLLHSSRADVNIRDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSEVGLKNKEGETPLECSAPNSDVWAALSKCQALPEKPTLQEKVVDRDISRGYENIPIPCVNAEDSELCPVNYKYVSQNCVTSPLNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKNGRLLPEFNMLEPPLIFECNHACSCWRNCRNRVVQNGLKIRLQLFRTKSKGWGVRSLQDIPQGTFVCEYVGELISDAEADVREDDTYLFDLDNKDREVYCIDARFYGNISRFINHLCEPNLLPVRVFMSHQDLRFPRIGFFSSRHIGAGEEIGFDYGDRFWDVKGKLFSCQCGSPKCKHPIKVLSPSQSFAPAETPSEIGLPDTSSATPPSPSQPQ

Target ORF information:

RefSeq Version	NM_001102698.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis euchromatic histone-lysine N-methyltransferase 1 (ehmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102698.1

ORF Insert Sequence:

ATGGCCGGCG ACGAAAACTT GTCGGACAAG CAGGTCGGTG AAACGAATGG ATCTTGTGAA 
AAGAACGAGT CCAAGGAGCT CGGTTTATTG ACGCTTGCCA CCCCAGACAG CACAAAACTC 
CCTCACCAGG ACTGTGTTTT TAAAACTTTT CGTGTGGCAG AGAACGGGGT GTACGACCGA 
GACACGGAGG GGCAGTACCC CTTAAAAGCG AGCAACCCCA CACAGACATC CGTAACGGGA 
AGTAATGGGT ACATTTTAAA CAAACCTGCC ATTTTGGAGC AGCCATTAAG GACTTCAAGC 
TTGTCACCTC TGAGTGGCCA TGCTGCTAAA ACTCTACCGG TTGCTGCCAG CAAACCCAAA 
GCAGCTTCTA ATGTCTACGT CCCTCCTGCC CCTTCTCCTG TCCCTCCGAA CCCTGGGGAT 
CCGGGGCGGG TTGAGAACAC AAAGCAACAG GACAGCGCTT TACCTGATCT GAAAGTTCAC 
CGTGCAAGGA AAACTCTACC GAAGACAAGC GGCAGTTCGC CCATCCATAA AGAGCCCAAA 
GAACCATCCG AGCAGGACGA AACCAAGGAC GAGAGTGCAC CCAAAAATGG TTTAGACGAC 
GAGAAAGAGG AACCCCAGTC TCCCTCCTCT GCCCCTCAAC AGCTGCTACC TCAGGGTCAA 
AACTCTGCCA AGTCGCTCTC AGCTGCACTG GTGTGTCGGA AGAAGAAACG GCGGATGGGC 
ATGTACAGCC TCGTACCCAA GAAGAAGACC AAAGTGCCAA AGCAAAGAAG CGTGCTGGAG 
ATGTTCCGGA ACATCACCTA CACCTCTTCC AGTCCCAAGA GCGAGAAAGA GTCCGATGTT 
CCTCATGTTA ACGGGGAAAG CATGGAGATG GATTCCGAAG ACGAGGATTC GGAAGACATG 
GAAGAAGAGG ACTACCACAG TTCGGAGACG CCAGCCTCGG AGAGTCAGGA AGAGCCCGGT 
GTTCCTAAAG AGGCCACCTC AGAAGCCGAC AGTTCCAGAA AGGTCGATGA GGTTGATTCT 
GAAGGAGATG AAGACGTGGA GTCTGGGGAT TCTGTAGAGG AGGAAGATGA CGGAGCAGAG 
TCTGATTTAA GCTCAGAGTC CAGTTTAAGG AAGAAGCAAG AGAAGAAGAA GACCCTGGCC 
GAAAGCCCCT GGATGAAGGG TTCTAGGCGA CGGAAGAGAA AGAACAAGGA AAATCCCAAT 
CCGCTTCTAG CTTCATCGGG AGAGGCTCGG GAAGCCAGCA TGGAATACAC AGAGGTCTCA 
TTAGACTCCT TGGATCTGCG CGTCAAAGAG ATGTTATCTC CGCAGTCTAA CGATCTCTCT 
AATGGCTCGG AGGCCATGGA ACTGGACGGC CTTCAGGGGA TCCCCCTCTG CAGCTGCCGG 
ATGGAGACCC CCAAAGAAGA GGAGGTGTCG GCAAAGGCCG GAGAGCATTG CATGGCCATT 
GAGAGCACTG GCCAAGAGCT GGGTCGCTGT ACCAACAGGG TGGCAAAGTA CGAGCTAATG 
AGGCCTTCTA ACAAGGTGCG TCATCTGGTG CTGTGTGAGG ATCACCGGGG CAAGATGGTC 
AAACACCAGT GTTGTCCTGG CTGCGGTTAC TTCTGCACAG CGGGGACCTT CATGGAATGC 
CAGCCGGACA GCACCATCTC CCACCGATTC CACAAGGACT GCGCCACCCG GGTCAACAAC 
TCGAGCTACT GCCCACACTG CGGGGAGGAG GCCTCCAAAG CGAAGGAGGT GACCATCGCC 
AAGGCCGACA CCACGTCCTC CCTGGCCCCA GTGACCGGCG AATGGGAGAA AGTCAGTGCG 
GCAGAGGGAC AGGCGGACAC TACAACCGAG AGCGAGGCCA TGGCTCTAAA GGCGCCAGAG 
GAGAATAAGC AAGAAGCAAC ATCTTCCCTC CCTGGAGGAA CCCCTGAGCC TATAGAGACC 
CCAAGTTTGG GCAAACCACC CTCTCTGTCT CAGGGGCCCG TCAAGGAGAC CTTGGAAAGC 
GCTCTACTCG CTTTGGACTC TGAAAAACCC AAGAAATTGC GGTTTCACCC AAAGCAGCTG 
TATTTTTCTG CCCGGCAAGG AGAGCTGCAG AAAGTCCTGA TGATGCTGGT GGACGGTATT 
GACCCCAACT ACAAAATGGA GCAGCACAGT CGGAGAACAC CACTTCACGC AGCGGCAGAG 
ATGGGTTACA CGGACATCTG CCACATGCTT ATACAGGCCG GAGCCAACCT AGACAGCTGC 
TCGGAGGATC AACGCACCCC CCTGATGGAG GCAGCTGAAA ATAATCATCT CGATACTGTG 
AAATACCTGG TGAAGGGCGG AGCTCTAATT GACCCTAAGG ACTCCGAGGG CTCCACTTGT 
CTTCATCTGG CGGCCAAGAA GGGTCACTAC GAAGTGGTGA AATATCTCCT CTCCTCCGAG 
AGAACCGACG TCAACTGCCA GGACGACGGG GGCTGGACCC CGATGATCTG GGCTACCGAG 
TACAAGCACG TGGAGATAGT CAAACTGTTA CATTCCTCCC GCGCGGACGT CAATATCCGC 
GATAATGAGG AGAACATCTG CCTGCACTGG GCCGCCTTCG CCGGTTCCGT CGAGATAGCG 
GAAATCCTCC TGGCTTCCAA ATGTGACCTC CGAGCTGTGA ATATCCACGG GGACTCCCCG 
CTCCATATTG CCGCCCGCGA GAACCGCCAC GAGTGCGTCA TGCTGTTCCT GGGTCACGGC 
TCGGAAGTGG GACTGAAGAA CAAAGAGGGC GAGACGCCGC TGGAGTGCTC GGCGCCCAAC 
TCCGACGTTT GGGCGGCCCT TAGCAAGTGC CAGGCCTTGC CCGAGAAGCC CACGCTGCAG 
GAAAAGGTGG TGGACAGGGA TATATCCCGG GGGTACGAGA ATATCCCCAT CCCTTGCGTC 
AACGCCGAAG ACTCCGAGCT GTGTCCCGTC AACTACAAAT ACGTCTCTCA GAACTGCGTG 
ACCTCCCCGC TGAACATCGA CAGAAACATT ACCCATCTGC AGTACTGTGT GTGCATCGAT 
GACTGTTCCT CTAGTAACTG TATGTGTGGC CAGCTGAGTA TGAGGTGCTG GTATGACAAG 
AACGGGCGCT TGTTACCGGA GTTCAACATG TTGGAGCCCC CGCTGATCTT CGAATGCAAC 
CACGCCTGCT CCTGCTGGCG CAACTGCCGC AACCGTGTGG TTCAAAATGG CTTGAAGATT 
AGATTGCAAC TGTTCCGCAC CAAAAGCAAA GGCTGGGGGG TGCGATCCCT GCAAGACATT 
CCCCAGGGGA CCTTCGTATG CGAGTATGTG GGGGAGCTGA TTTCGGACGC AGAGGCTGAC 
GTTCGAGAGG ATGACACCTA CCTCTTTGAT CTTGATAACA AGGACCGAGA GGTTTACTGC 
ATCGACGCCC GTTTCTACGG CAACATCAGT CGCTTTATCA ACCACCTCTG CGAGCCGAAC 
CTGCTCCCCG TGCGAGTCTT TATGTCCCAC CAGGATCTGC GCTTTCCCCG CATCGGCTTC 
TTCAGCAGCC GGCACATCGG CGCGGGGGAG GAGATCGGGT TTGATTACGG CGACCGATTC 
TGGGACGTCA AGGGGAAGCT CTTCAGCTGC CAGTGCGGTT CCCCCAAGTG CAAGCACCCA 
ATCAAAGTCC TTTCCCCGAG CCAGTCATTC GCCCCCGCAG AAACTCCGTC GGAGATTGGC 
TTACCCGACA CCAGCTCAGC CACCCCCCCG AGCCCCTCGC AGCCGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ehmt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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