Ehmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ehmt1
Entrez Gene ID	362078
Full Name	euchromatic histone lysine methyltransferase 1
General protein information	Preferred Names euchromatic histone lysine methyltransferase 1 Names histone-lysine N-methyltransferase EHMT1 euchromatic histone methyltransferase 1 euchromatic histone-lysine N-methyltransferase 1 histone-lysine N-methyltransferase, H3 lysine-9 specific 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233619.3	XP_006233681.2	histone-lysine N-methyltransferase EHMT1 isoform X2
NM_001108572.1	NP_001102042.1	histone-lysine N-methyltransferase EHMT1
XM_006233618.3	XP_006233680.2	histone-lysine N-methyltransferase EHMT1 isoform X1
XM_017591873.1	XP_017447362.1	histone-lysine N-methyltransferase EHMT1 isoform X5
XM_017591871.1	XP_017447360.1	histone-lysine N-methyltransferase EHMT1 isoform X3
XM_017591872.1	XP_017447361.1	histone-lysine N-methyltransferase EHMT1 isoform X4
XM_017591874.1	XP_017447363.1	histone-lysine N-methyltransferase EHMT1 isoform X6

Pathways from BioSystems

Homologies

Homo sapiens (human)	EHMT1	NP_079033.4
Pan troglodytes (chimpanzee)	EHMT1	XP_520395.3
Bos taurus (cattle)	EHMT1	NP_001092511.1
Mus musculus (house mouse)	Ehmt1	NP_001012536.2
Rattus norvegicus (Norway rat)	Ehmt1	NP_001102042.1
Gallus gallus (chicken)	EHMT1	NP_001012550.1
Xenopus tropicalis (tropical clawed frog)	ehmt1	NP_001096168.1
Danio rerio (zebrafish)	ehmt1b	NP_001166035.1
Canis lupus familiaris (dog)	EHMT1	XP_005625091.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
Sharma M, Dierkes T, Sajikumar S
Aging cell16(5)1062-1072(2017 Oct)

The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.
Thienpont B, Aronsen JM, Robinson EL, Okkenhaug H, Loche E, Ferrini A, Brien P, Alkass K, Tomasso A, Agrawal A, Bergmann O, Sjaastad I, Reik W, Roderick HL
The Journal of clinical investigation127(1)335-348(2017 Jan)

Overexpression of G9a and MCM7 in oesophageal squamous cell carcinoma is associated with poor prognosis.
Zhong X, Chen X, Guan X, Zhang H, Ma Y, Zhang S, Wang E, Zhang L, Han Y
Histopathology66(2)192-200(2015 Jan)

Analysis of EHMT1 expression and its correlations with clinical significance in esophageal squamous cell cancer.
Guan X, Zhong X, Men W, Gong S, Zhang L, Han Y
Molecular and clinical oncology2(1)76-80(2014 Jan)

EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex.
Ohno H, Shinoda K, Ohyama K, Sharp LZ, Kajimura S
Nature504(7478)163-7(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-217-mediated, genetic, or pharmacological inactivation of EHMT1/2 was sufficient to promote pathological hypertrophy
Title: The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.

The following Ehmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ehmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11776	NM_001108572.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$937.30 $1339.00
ORa48123	XM_006233618.3 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa48124	XM_006233619.3 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa60463	XM_017591871.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa60464	XM_017591872.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa60465	XM_017591873.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa60466	XM_017591874.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ehmt1 ( NM_001108572.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102042.1 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), mRNA.

Price & Availability↑

CloneID	ORa11776
Clone ID Related Accession (Same CDS sequence)	NM_001108572.1
Accession Version	NM_001108572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-06
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474001.2. On or before Oct 4, 2007 this sequence version replaced XM_001077166.1, XM_342379.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCCG CGGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAACTGCT AAGGGAAGAT ACACTTATGG CTGCTGATGA AGGTTCCACA 
GAGAAGCAAG CAGGAGAGAC TCCTATGGCT GCAGATGGAG AAACCAATGG GTCTTGTGAA 
AAGAGTGGCG ATATCAGCCA TCCAAATGCA CCCAAACATA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTATCT CGGGTGGCGG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTT ACAGCTGACG ACTTCATGCA GACGTCTGTC 
ATTGGAAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGACAGCC CTTGAGGACT 
CCCAACACTC TAAATTCCTC GCTTCCTGGT CATGCCGCAA AAACGCTTCC TGGAGGAGCC 
AGTAAGTGCA GGACTCCAAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCCTGG GCCTGCACGC AGCCAGTAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACT TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAATGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTGT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGCTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAGCA GAGGACGGTG ATTGAGATGT TTAAAAGCAT CACTCATTCC 
ACTGTGGGTG CCAAGGGTGA GAAAGTCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCCGA GGAGGAAGAC TCTGAGGAGC TGGAGGATGA GGAGGACCGT 
GGCGCTGAGC AGGCTGCTGC ATTTCCCACT GAGGACAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ATCGTGCTGC AAAGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATCCT CCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAC GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTTTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGCCTCA ATTCCCAGGT GTGGAGTGCA 
CTGCAGATGA GCAAGGCACT TCAGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GAGACATTGC CCGAGGATAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGATAGTG AGTTATGTCC CACCAACTAC AAGTACGTCT CCCAGAACTG CGTGACGTCC 
CCAATGAACA TCGACAGGAA CATAACTCAT TTGCAGTACT GCGTTTGTGT AGACGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA CAAGGACGGG 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCCTGCT GGAGGAACTG CCGCAATCGC GTGGTGCAGA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTTCAGGA TATCCCGCTG 
GGCACCTTTG TCTGCGAATA CGTGGGGGAG CTGATTTCAG ACTCCGAGGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTGTA CTGCATTGAT 
GCTCGGTTCT ACGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAGCC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTACGGTTTC CCAGGATTGC CTTCTTCAGC 
ACCCGCCTGA TTCAGGCTGG GGAACAGCTG GGGTTTGACT ATGGGGAACG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTCCCA AGTGTCGGCA CTCAAGTGCA 
GCCCTGGCCC AGCGGCAAGC CAGTGCAGCC CAGGAGGCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCTGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102042.1
CDS	11..3823
Translation	MAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	NM_001108572.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108572.1

ORF Insert Sequence:

ATGGCCGCCG CGGATGCTGA GCAGGCAGTT CTGGCCAAGC AAGAGACCAA GCAGGATTGC 
TGCATGAAAA CTGAACTGCT AAGGGAAGAT ACACTTATGG CTGCTGATGA AGGTTCCACA 
GAGAAGCAAG CAGGAGAGAC TCCTATGGCT GCAGATGGAG AAACCAATGG GTCTTGTGAA 
AAGAGTGGCG ATATCAGCCA TCCAAATGCA CCCAAACATA CTCAGGAGAA CACAAGAGCT 
AGCCCACAGG AAGGCACCAA CAGAGTATCT CGGGTGGCGG AAAATGGGGT TTCAGAAAGA 
GACACAGAAG TGGGGAAGCA AAACCATGTT ACAGCTGACG ACTTCATGCA GACGTCTGTC 
ATTGGAAGCA ATGGATATTT CTTAAATAAA CCAGCCCTGC AGGGACAGCC CTTGAGGACT 
CCCAACACTC TAAATTCCTC GCTTCCTGGT CATGCCGCAA AAACGCTTCC TGGAGGAGCC 
AGTAAGTGCA GGACTCCAAG TGCACTTCCT CAGACACCAA CCACAGCACC CACTGTGCCT 
GGGGAAGGGA GTGCAGACAC AGAGGACAGA AAGCCTACAG CCTCGGGCAC TGATGTCAGG 
GTTCACAGGG CACGCAAGAC CATGCCGAAG TCCATCCTGG GCCTGCACGC AGCCAGTAAA 
GACCATAGAG AAGTTCAAGA CCATAAGGAA CCAAAAGAGG ATATCAACAG AAACATTTCT 
GAATGTGGAC GACAGCAGCT TTTACCAACT TTCCCAGCCC TCCACCAGTC GCTACCTCAG 
AATCAATGCT ACATGGCCAC CACAAAGTCC CAGACAGCTT GCTTGCCTTT TGTTTTAGCA 
GCTGCAGTGT CTCGGAAGAA AAAACGAAGA ATGGGAACCT ATAGCTTAGT TCCCAAGAAA 
AAGACCAAAG TATTAAAGCA GAGGACGGTG ATTGAGATGT TTAAAAGCAT CACTCATTCC 
ACTGTGGGTG CCAAGGGTGA GAAAGTCTTA GATGATAGTG CCCTGCATGT AAATGGCGAG 
AGCTTGGAGA TGGACTCCGA GGAGGAAGAC TCTGAGGAGC TGGAGGATGA GGAGGACCGT 
GGCGCTGAGC AGGCTGCTGC ATTTCCCACT GAGGACAGTA GGACTTCTAA AGAGAGCATG 
TCTGAGACTG ATCGTGCTGC AAAGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATCCT CCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAC GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTTTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGCCTCA ATTCCCAGGT GTGGAGTGCA 
CTGCAGATGA GCAAGGCACT TCAGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GAGACATTGC CCGAGGATAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGATAGTG AGTTATGTCC CACCAACTAC AAGTACGTCT CCCAGAACTG CGTGACGTCC 
CCAATGAACA TCGACAGGAA CATAACTCAT TTGCAGTACT GCGTTTGTGT AGACGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA CAAGGACGGG 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCCTGCT GGAGGAACTG CCGCAATCGC GTGGTGCAGA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTTCAGGA TATCCCGCTG 
GGCACCTTTG TCTGCGAATA CGTGGGGGAG CTGATTTCAG ACTCCGAGGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTGTA CTGCATTGAT 
GCTCGGTTCT ACGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAGCC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTACGGTTTC CCAGGATTGC CTTCTTCAGC 
ACCCGCCTGA TTCAGGCTGG GGAACAGCTG GGGTTTGACT ATGGGGAACG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTCCCA AGTGTCGGCA CTCAAGTGCA 
GCCCTGGCCC AGCGGCAAGC CAGTGCAGCC CAGGAGGCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCTGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006233618.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233680.2 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa48123
Clone ID Related Accession (Same CDS sequence)	XM_006233618.3
Accession Version	XM_006233618.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATCCT CCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAC GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTTTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGCCTCA ATTCCCAGGT GTGGAGTGCA 
CTGCAGATGA GCAAGGCACT TCAGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GAGACATTGC CCGAGGATAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGATAGTG AGTTATGTCC CACCAACTAC AAGTACGTCT CCCAGAACTG CGTGACGTCC 
CCAATGAACA TCGACAGGAA CATAACTCAT TTGCAGTACT GCGTTTGTGT AGACGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA CAAGGACGGG 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCCTGCT GGAGGAACTG CCGCAATCGC GTGGTGCAGA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTTCAGGA TATCCCGCTG 
GGCACCTTTG TCTGCGAATA CGTGGGGGAG CTGATTTCAG ACTCCGAGGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTGTA CTGCATTGAT 
GCTCGGTTCT ACGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAGCC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTACGGTTTC CCAGGATTGC CTTCTTCAGC 
ACCCGCCTGA TTCAGGCTGG GGAACAGCTG GGGTTTGACT ATGGGGAACG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTCCCA AGTGTCGGCA CTCAAGTGCA 
GCCCTGGCCC AGCGGCAAGC CAGTGCAGCC CAGGAGGCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCTGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233680.2
CDS	21..3953
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006233618.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233618.3

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATGA GGAGAACATT 
TGTCTGCACT GGGCAGCATT TTCAGGCTGT GTGGACATAG CTGAAATCCT CCTGGCTGCC 
AAGTGTGACC TGCATGCTGT GAATATCCAC GGAGACTCAC CCCTGCACAT CGCAGCCAGG 
GAGAATCGCT ACGACTGTGT TGTCCTCTTT CTTTCTCGGG ATTCAGATGT TACTTTGAAA 
AACAAGGAAG GAGAGACTCC CTTGCAGTGT GCAAGCCTCA ATTCCCAGGT GTGGAGTGCA 
CTGCAGATGA GCAAGGCACT TCAGGACTCA GCCCCTGACA AGCCCGTTGC TGTTGAGAAG 
ACGGTGAGCA GAGACATTGC CCGAGGATAT GAGCGCATTC CCATTCCCTG TGTCAATGCT 
GTGGATAGTG AGTTATGTCC CACCAACTAC AAGTACGTCT CCCAGAACTG CGTGACGTCC 
CCAATGAACA TCGACAGGAA CATAACTCAT TTGCAGTACT GCGTTTGTGT AGACGACTGC 
TCCTCTAGCA CCTGCATGTG TGGCCAGCTG AGCATGCGCT GCTGGTATGA CAAGGACGGG 
CGACTTCTGC CTGAGTTTAA CATGGCAGAA CCACCCTTGA TCTTCGAGTG CAATCATGCC 
TGCTCCTGCT GGAGGAACTG CCGCAATCGC GTGGTGCAGA ATGGTCTCAG GGCAAGGCTG 
CAGCTTTATC GGACACAGGA CATGGGCTGG GGTGTGCGGT CCCTTCAGGA TATCCCGCTG 
GGCACCTTTG TCTGCGAATA CGTGGGGGAG CTGATTTCAG ACTCCGAGGC TGATGTTCGG 
GAAGAGGACT CTTACCTCTT TGATCTTGAC AATAAGGATG GAGAGGTGTA CTGCATTGAT 
GCTCGGTTCT ACGGGAATGT CAGCCGGTTC ATAAACCACC ACTGCGAGCC CAACCTTGTG 
CCTGTGCGAG TGTTCATGTC ACACCAGGAC CTACGGTTTC CCAGGATTGC CTTCTTCAGC 
ACCCGCCTGA TTCAGGCTGG GGAACAGCTG GGGTTTGACT ATGGGGAACG CTTTTGGGAC 
GTCAAGGGCA AGCTCTTCAG TTGCCGGTGT GGGTCTCCCA AGTGTCGGCA CTCAAGTGCA 
GCCCTGGCCC AGCGGCAAGC CAGTGCAGCC CAGGAGGCTC AGGAGAATGG CCTTCCAGAT 
ACCAGCTCTG CAGCTGCTGC TGACCCCCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_006233619.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233681.2 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.

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CloneID	ORa48124
Clone ID Related Accession (Same CDS sequence)	XM_006233619.3
Accession Version	XM_006233619.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAACTGCTA 
AGGGAAGATA CACTTATGGC TGCTGATGAA GGTTCCACAG AGAAGCAAGC AGGAGAGACT 
CCTATGGCTG CAGATGGAGA AACCAATGGG TCTTGTGAAA AGAGTGGCGA TATCAGCCAT 
CCAAATGCAC CCAAACATAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTATCTC GGGTGGCGGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTTA CAGCTGACGA CTTCATGCAG ACGTCTGTCA TTGGAAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGACAGCCC TTGAGGACTC CCAACACTCT AAATTCCTCG 
CTTCCTGGTC ATGCCGCAAA AACGCTTCCT GGAGGAGCCA GTAAGTGCAG GACTCCAAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCCTGGG CCTGCACGCA GCCAGTAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACTT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAATGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTGTC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGCTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAGCAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATC ACTCATTCCA CTGTGGGTGC CAAGGGTGAG 
AAAGTCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCCGAG 
GAGGAAGACT CTGAGGAGCT GGAGGATGAG GAGGACCGTG GCGCTGAGCA GGCTGCTGCA 
TTTCCCACTG AGGACAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA TCGTGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCTG ACACAGGGGA GGACGAAGAC 
GGTGGAGATG AGTCTGACCT GAGTTCTGAA TCTAGTATCA AGAAGAAATT TCTCAAGAGA 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGCAGA 
AAGAAGCCAA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCCCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGGTAT ATGGAAGTTT CTCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACG GAAAACGAAG GGCTGGCCAA TGGTCCGGAT 
GTGCTGGAGA CGGATGGCCT CCATGAAGTG CCTCTCTGCA GCTGCCGCAT GGAAACCCCC 
AAGAGCCGTG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCTTCCAAC 
AAAGCACCAC TCTTGGTGCT GTGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGTCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAGAC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACG 
ACTTCCACAG TGACCCTGGC CCCTGGACAG GAGAAGAGCT TGGCTGCTGA GGGCAGGGCT 
GACACGACCA CTGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAAATCGCA GAGCACAGTC 
ACTCAGGCAC CAGAGTGCTT CGATCCTGCA GGGCCAGCTG GTTTCGTGAG GCCGACCTCT 
GGCTTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTCATTGC TCTGGACTCT 
GAAAAACCGA AGAAACTTCG CTTCCACCCG AAGCAGCTGT ACTTCTCCGC TAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACACGCT GCTGCAGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GAGGACCCCC 
CTGATGGAGG CCGCAGAGAA CAACCACTTG GACGCAGTGA AGTACCTCAT CAAGGCCGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACGTGTT TGCACTTGGC TGCCAAGAAA 
GGCCACTATG ACGTGGTTCA GTATCTGCTG TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGATGGTG GTTGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGACCTGGTG 
AAGCTGCTGC TCTCTAAAGG CTCTGACATC AATATCCGGG ACAATGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATCCTCCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCACGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TTTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGCCTCAATT CCCAGGTGTG GAGTGCACTG 
CAGATGAGCA AGGCACTTCA GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGAG ACATTGCCCG AGGATATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GATAGTGAGT TATGTCCCAC CAACTACAAG TACGTCTCCC AGAACTGCGT GACGTCCCCA 
ATGAACATCG ACAGGAACAT AACTCATTTG CAGTACTGCG TTTGTGTAGA CGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGACAA GGACGGGCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCCTGCTGGA GGAACTGCCG CAATCGCGTG GTGCAGAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TTCAGGATAT CCCGCTGGGC 
ACCTTTGTCT GCGAATACGT GGGGGAGCTG ATTTCAGACT CCGAGGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTGTACTG CATTGATGCT 
CGGTTCTACG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAGCCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTA CGGTTTCCCA GGATTGCCTT CTTCAGCACC 
CGCCTGATTC AGGCTGGGGA ACAGCTGGGG TTTGACTATG GGGAACGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTCCCAAGT GTCGGCACTC AAGTGCAGCC 
CTGGCCCAGC GGCAAGCCAG TGCAGCCCAG GAGGCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CTGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233681.2
CDS	16..3945
Translation	MARGRGGATLRARAMAAADAEAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_006233619.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233619.3

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGGCAGTTC TGGCCAAGCA AGAGACCAAG CAGGATTGCT GCATGAAAAC TGAACTGCTA 
AGGGAAGATA CACTTATGGC TGCTGATGAA GGTTCCACAG AGAAGCAAGC AGGAGAGACT 
CCTATGGCTG CAGATGGAGA AACCAATGGG TCTTGTGAAA AGAGTGGCGA TATCAGCCAT 
CCAAATGCAC CCAAACATAC TCAGGAGAAC ACAAGAGCTA GCCCACAGGA AGGCACCAAC 
AGAGTATCTC GGGTGGCGGA AAATGGGGTT TCAGAAAGAG ACACAGAAGT GGGGAAGCAA 
AACCATGTTA CAGCTGACGA CTTCATGCAG ACGTCTGTCA TTGGAAGCAA TGGATATTTC 
TTAAATAAAC CAGCCCTGCA GGGACAGCCC TTGAGGACTC CCAACACTCT AAATTCCTCG 
CTTCCTGGTC ATGCCGCAAA AACGCTTCCT GGAGGAGCCA GTAAGTGCAG GACTCCAAGT 
GCACTTCCTC AGACACCAAC CACAGCACCC ACTGTGCCTG GGGAAGGGAG TGCAGACACA 
GAGGACAGAA AGCCTACAGC CTCGGGCACT GATGTCAGGG TTCACAGGGC ACGCAAGACC 
ATGCCGAAGT CCATCCTGGG CCTGCACGCA GCCAGTAAAG ACCATAGAGA AGTTCAAGAC 
CATAAGGAAC CAAAAGAGGA TATCAACAGA AACATTTCTG AATGTGGACG ACAGCAGCTT 
TTACCAACTT TCCCAGCCCT CCACCAGTCG CTACCTCAGA ATCAATGCTA CATGGCCACC 
ACAAAGTCCC AGACAGCTTG CTTGCCTTTT GTTTTAGCAG CTGCAGTGTC TCGGAAGAAA 
AAACGAAGAA TGGGAACCTA TAGCTTAGTT CCCAAGAAAA AGACCAAAGT ATTAAAGCAG 
AGGACGGTGA TTGAGATGTT TAAAAGCATC ACTCATTCCA CTGTGGGTGC CAAGGGTGAG 
AAAGTCTTAG ATGATAGTGC CCTGCATGTA AATGGCGAGA GCTTGGAGAT GGACTCCGAG 
GAGGAAGACT CTGAGGAGCT GGAGGATGAG GAGGACCGTG GCGCTGAGCA GGCTGCTGCA 
TTTCCCACTG AGGACAGTAG GACTTCTAAA GAGAGCATGT CTGAGACTGA TCGTGCTGCA 
AAGATGGATG GAGATTCAGA GGAGGAACAG GAGTCTCCTG ACACAGGGGA GGACGAAGAC 
GGTGGAGATG AGTCTGACCT GAGTTCTGAA TCTAGTATCA AGAAGAAATT TCTCAAGAGA 
AGAGGGAAGA CTGACAGCCC CTGGATCAAA CCTGCTCGGA AAAGGAGGCG GAGAAGCAGA 
AAGAAGCCAA GCAGCATGCT TGGTTCCGAG GCTTGTAAGT CATCCCCAGG AAGCATGGAG 
CAGGCAGCTC TGGGAGACAG CGCTGGGTAT ATGGAAGTTT CTCTGGATTC CCTGGATCTC 
CGTGTCAGAG GAATTCTGTC TTCCCAGACG GAAAACGAAG GGCTGGCCAA TGGTCCGGAT 
GTGCTGGAGA CGGATGGCCT CCATGAAGTG CCTCTCTGCA GCTGCCGCAT GGAAACCCCC 
AAGAGCCGTG AGATCAGCAC CCTGGCCAAC AACCAGTGCA TGGCCACTGA GAGCGTGGAT 
CACGAATTGG GCCGGTGTAC AAACAGCGTG GTCAAGTATG AGCTGATGCG CCCTTCCAAC 
AAAGCACCAC TCTTGGTGCT GTGTGAAGAC CATCGGGGTC GCATGGTGAA GCACCAGTGC 
TGTCCTGGCT GCGGCTACTT CTGCACGGCG GGTAACTTCA TGGAATGTCA GCCTGAGAGC 
AGCATCTCTC ATCGTTTCCA TAAGGACTGT GCCTCTCGAG TCAACAATGC CAGCTACTGT 
CCCCATTGTG GGGAAGAGAC TTCCAAGGCC AAAGAGGTGA CCATAGCAAA AGCAGACACG 
ACTTCCACAG TGACCCTGGC CCCTGGACAG GAGAAGAGCT TGGCTGCTGA GGGCAGGGCT 
GACACGACCA CTGGCAGCAT TGCTGGAGCC CCAGAGGATG AAAAATCGCA GAGCACAGTC 
ACTCAGGCAC CAGAGTGCTT CGATCCTGCA GGGCCAGCTG GTTTCGTGAG GCCGACCTCT 
GGCTTTTCCC AGGGCCCAGG AAAGGAAACC TTGGAAAGTG CTCTCATTGC TCTGGACTCT 
GAAAAACCGA AGAAACTTCG CTTCCACCCG AAGCAGCTGT ACTTCTCCGC TAGGCAGGGT 
GAGCTGCAGA AGGTGCTTCT CATGCTGGTT GATGGAATTG ATCCCAACTT CAAAATGGAG 
CACCAAAGTA AGCGTTCCCC ATTACACGCT GCTGCAGAGG CTGGCCACGT GGACATCTGC 
CACATGCTGG TTCAGGCGGG TGCCAATATT GACACTTGCT CAGAGGACCA GAGGACCCCC 
CTGATGGAGG CCGCAGAGAA CAACCACTTG GACGCAGTGA AGTACCTCAT CAAGGCCGGA 
GCACAGGTGG ATCCGAAGGA CGCAGAGGGC TCCACGTGTT TGCACTTGGC TGCCAAGAAA 
GGCCACTATG ACGTGGTTCA GTATCTGCTG TCAAATGGAC AGATGGATGT CAACTGCCAG 
GATGATGGTG GTTGGACACC TATGATCTGG GCCACTGAGT ACAAGCACGT GGACCTGGTG 
AAGCTGCTGC TCTCTAAAGG CTCTGACATC AATATCCGGG ACAATGAGGA GAACATTTGT 
CTGCACTGGG CAGCATTTTC AGGCTGTGTG GACATAGCTG AAATCCTCCT GGCTGCCAAG 
TGTGACCTGC ATGCTGTGAA TATCCACGGA GACTCACCCC TGCACATCGC AGCCAGGGAG 
AATCGCTACG ACTGTGTTGT CCTCTTTCTT TCTCGGGATT CAGATGTTAC TTTGAAAAAC 
AAGGAAGGAG AGACTCCCTT GCAGTGTGCA AGCCTCAATT CCCAGGTGTG GAGTGCACTG 
CAGATGAGCA AGGCACTTCA GGACTCAGCC CCTGACAAGC CCGTTGCTGT TGAGAAGACG 
GTGAGCAGAG ACATTGCCCG AGGATATGAG CGCATTCCCA TTCCCTGTGT CAATGCTGTG 
GATAGTGAGT TATGTCCCAC CAACTACAAG TACGTCTCCC AGAACTGCGT GACGTCCCCA 
ATGAACATCG ACAGGAACAT AACTCATTTG CAGTACTGCG TTTGTGTAGA CGACTGCTCC 
TCTAGCACCT GCATGTGTGG CCAGCTGAGC ATGCGCTGCT GGTATGACAA GGACGGGCGA 
CTTCTGCCTG AGTTTAACAT GGCAGAACCA CCCTTGATCT TCGAGTGCAA TCATGCCTGC 
TCCTGCTGGA GGAACTGCCG CAATCGCGTG GTGCAGAATG GTCTCAGGGC AAGGCTGCAG 
CTTTATCGGA CACAGGACAT GGGCTGGGGT GTGCGGTCCC TTCAGGATAT CCCGCTGGGC 
ACCTTTGTCT GCGAATACGT GGGGGAGCTG ATTTCAGACT CCGAGGCTGA TGTTCGGGAA 
GAGGACTCTT ACCTCTTTGA TCTTGACAAT AAGGATGGAG AGGTGTACTG CATTGATGCT 
CGGTTCTACG GGAATGTCAG CCGGTTCATA AACCACCACT GCGAGCCCAA CCTTGTGCCT 
GTGCGAGTGT TCATGTCACA CCAGGACCTA CGGTTTCCCA GGATTGCCTT CTTCAGCACC 
CGCCTGATTC AGGCTGGGGA ACAGCTGGGG TTTGACTATG GGGAACGCTT TTGGGACGTC 
AAGGGCAAGC TCTTCAGTTG CCGGTGTGGG TCTCCCAAGT GTCGGCACTC AAGTGCAGCC 
CTGGCCCAGC GGCAAGCCAG TGCAGCCCAG GAGGCTCAGG AGAATGGCCT TCCAGATACC 
AGCTCTGCAG CTGCTGCTGA CCCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_017591871.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447360.1 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa60463
Clone ID Related Accession (Same CDS sequence)	XM_017591871.1
Accession Version	XM_017591871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTG TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGCTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAGC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TCACTCATTC CACTGTGGGT GCCAAGGGTG AGAAAGTCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCCG AGGAGGAAGA CTCTGAGGAG 
CTGGAGGATG AGGAGGACCG TGGCGCTGAG CAGGCTGCTG CATTTCCCAC TGAGGACAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GATCGTGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC TGACACAGGG GAGGACGAAG ACGGTGGAGA TGAGTCTGAC 
CTGAGTTCTG AATCTAGTAT CAAGAAGAAA TTTCTCAAGA GAAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGCA GAAAGAAGCC AAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCCCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGGT ATATGGAAGT TTCTCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CGGAAAACGA AGGGCTGGCC AATGGTCCGG ATGTGCTGGA GACGGATGGC 
CTCCATGAAG TGCCTCTCTG CAGCTGCCGC ATGGAAACCC CCAAGAGCCG TGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCTTCCA ACAAAGCACC ACTCTTGGTG 
CTGTGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGT CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAG 
ACTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CGACTTCCAC AGTGACCCTG 
GCCCCTGGAC AGGAGAAGAG CTTGGCTGCT GAGGGCAGGG CTGACACGAC CACTGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAAATCG CAGAGCACAG TCACTCAGGC ACCAGAGTGC 
TTCGATCCTG CAGGGCCAGC TGGTTTCGTG AGGCCGACCT CTGGCTTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTCATT GCTCTGGACT CTGAAAAACC GAAGAAACTT 
CGCTTCCACC CGAAGCAGCT GTACTTCTCC GCTAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACACG CTGCTGCAGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGAGGACCC CCCTGATGGA GGCCGCAGAG 
AACAACCACT TGGACGCAGT GAAGTACCTC ATCAAGGCCG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACGTG TTTGCACTTG GCTGCCAAGA AAGGCCACTA TGACGTGGTT 
CAGTATCTGC TGTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGATGG TGGTTGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTGG TGAAGCTGCT GCTCTCTAAA 
GGCTCTGACA TCAATATCCG GGACAATGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATCCTC CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCACG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTTTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGCCTCAA TTCCCAGGTG TGGAGTGCAC TGCAGATGAG CAAGGCACTT 
CAGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG AGACATTGCC 
CGAGGATATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGATAGTGA GTTATGTCCC 
ACCAACTACA AGTACGTCTC CCAGAACTGC GTGACGTCCC CAATGAACAT CGACAGGAAC 
ATAACTCATT TGCAGTACTG CGTTTGTGTA GACGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAC AAGGACGGGC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCCTGCTG GAGGAACTGC 
CGCAATCGCG TGGTGCAGAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTTCAGGAT ATCCCGCTGG GCACCTTTGT CTGCGAATAC 
GTGGGGGAGC TGATTTCAGA CTCCGAGGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTGTAC TGCATTGATG CTCGGTTCTA CGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAGCCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TACGGTTTCC CAGGATTGCC TTCTTCAGCA CCCGCCTGAT TCAGGCTGGG 
GAACAGCTGG GGTTTGACTA TGGGGAACGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTCCCAA GTGTCGGCAC TCAAGTGCAG CCCTGGCCCA GCGGCAAGCC 
AGTGCAGCCC AGGAGGCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCTGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447360.1
CDS	21..3932
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_017591871.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591871.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTG TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGCTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAGC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TCACTCATTC CACTGTGGGT GCCAAGGGTG AGAAAGTCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCCG AGGAGGAAGA CTCTGAGGAG 
CTGGAGGATG AGGAGGACCG TGGCGCTGAG CAGGCTGCTG CATTTCCCAC TGAGGACAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GATCGTGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC TGACACAGGG GAGGACGAAG ACGGTGGAGA TGAGTCTGAC 
CTGAGTTCTG AATCTAGTAT CAAGAAGAAA TTTCTCAAGA GAAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGCA GAAAGAAGCC AAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCCCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGGT ATATGGAAGT TTCTCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CGGAAAACGA AGGGCTGGCC AATGGTCCGG ATGTGCTGGA GACGGATGGC 
CTCCATGAAG TGCCTCTCTG CAGCTGCCGC ATGGAAACCC CCAAGAGCCG TGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAATT GGGCCGGTGT 
ACAAACAGCG TGGTCAAGTA TGAGCTGATG CGCCCTTCCA ACAAAGCACC ACTCTTGGTG 
CTGTGTGAAG ACCATCGGGG TCGCATGGTG AAGCACCAGT GCTGTCCTGG CTGCGGCTAC 
TTCTGCACGG CGGGTAACTT CATGGAATGT CAGCCTGAGA GCAGCATCTC TCATCGTTTC 
CATAAGGACT GTGCCTCTCG AGTCAACAAT GCCAGCTACT GTCCCCATTG TGGGGAAGAG 
ACTTCCAAGG CCAAAGAGGT GACCATAGCA AAAGCAGACA CGACTTCCAC AGTGACCCTG 
GCCCCTGGAC AGGAGAAGAG CTTGGCTGCT GAGGGCAGGG CTGACACGAC CACTGGCAGC 
ATTGCTGGAG CCCCAGAGGA TGAAAAATCG CAGAGCACAG TCACTCAGGC ACCAGAGTGC 
TTCGATCCTG CAGGGCCAGC TGGTTTCGTG AGGCCGACCT CTGGCTTTTC CCAGGGCCCA 
GGAAAGGAAA CCTTGGAAAG TGCTCTCATT GCTCTGGACT CTGAAAAACC GAAGAAACTT 
CGCTTCCACC CGAAGCAGCT GTACTTCTCC GCTAGGCAGG GTGAGCTGCA GAAGGTGCTT 
CTCATGCTGG TTGATGGAAT TGATCCCAAC TTCAAAATGG AGCACCAAAG TAAGCGTTCC 
CCATTACACG CTGCTGCAGA GGCTGGCCAC GTGGACATCT GCCACATGCT GGTTCAGGCG 
GGTGCCAATA TTGACACTTG CTCAGAGGAC CAGAGGACCC CCCTGATGGA GGCCGCAGAG 
AACAACCACT TGGACGCAGT GAAGTACCTC ATCAAGGCCG GAGCACAGGT GGATCCGAAG 
GACGCAGAGG GCTCCACGTG TTTGCACTTG GCTGCCAAGA AAGGCCACTA TGACGTGGTT 
CAGTATCTGC TGTCAAATGG ACAGATGGAT GTCAACTGCC AGGATGATGG TGGTTGGACA 
CCTATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTGG TGAAGCTGCT GCTCTCTAAA 
GGCTCTGACA TCAATATCCG GGACAATGAG GAGAACATTT GTCTGCACTG GGCAGCATTT 
TCAGGCTGTG TGGACATAGC TGAAATCCTC CTGGCTGCCA AGTGTGACCT GCATGCTGTG 
AATATCCACG GAGACTCACC CCTGCACATC GCAGCCAGGG AGAATCGCTA CGACTGTGTT 
GTCCTCTTTC TTTCTCGGGA TTCAGATGTT ACTTTGAAAA ACAAGGAAGG AGAGACTCCC 
TTGCAGTGTG CAAGCCTCAA TTCCCAGGTG TGGAGTGCAC TGCAGATGAG CAAGGCACTT 
CAGGACTCAG CCCCTGACAA GCCCGTTGCT GTTGAGAAGA CGGTGAGCAG AGACATTGCC 
CGAGGATATG AGCGCATTCC CATTCCCTGT GTCAATGCTG TGGATAGTGA GTTATGTCCC 
ACCAACTACA AGTACGTCTC CCAGAACTGC GTGACGTCCC CAATGAACAT CGACAGGAAC 
ATAACTCATT TGCAGTACTG CGTTTGTGTA GACGACTGCT CCTCTAGCAC CTGCATGTGT 
GGCCAGCTGA GCATGCGCTG CTGGTATGAC AAGGACGGGC GACTTCTGCC TGAGTTTAAC 
ATGGCAGAAC CACCCTTGAT CTTCGAGTGC AATCATGCCT GCTCCTGCTG GAGGAACTGC 
CGCAATCGCG TGGTGCAGAA TGGTCTCAGG GCAAGGCTGC AGCTTTATCG GACACAGGAC 
ATGGGCTGGG GTGTGCGGTC CCTTCAGGAT ATCCCGCTGG GCACCTTTGT CTGCGAATAC 
GTGGGGGAGC TGATTTCAGA CTCCGAGGCT GATGTTCGGG AAGAGGACTC TTACCTCTTT 
GATCTTGACA ATAAGGATGG AGAGGTGTAC TGCATTGATG CTCGGTTCTA CGGGAATGTC 
AGCCGGTTCA TAAACCACCA CTGCGAGCCC AACCTTGTGC CTGTGCGAGT GTTCATGTCA 
CACCAGGACC TACGGTTTCC CAGGATTGCC TTCTTCAGCA CCCGCCTGAT TCAGGCTGGG 
GAACAGCTGG GGTTTGACTA TGGGGAACGC TTTTGGGACG TCAAGGGCAA GCTCTTCAGT 
TGCCGGTGTG GGTCTCCCAA GTGTCGGCAC TCAAGTGCAG CCCTGGCCCA GCGGCAAGCC 
AGTGCAGCCC AGGAGGCTCA GGAGAATGGC CTTCCAGATA CCAGCTCTGC AGCTGCTGCT 
GACCCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_017591872.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447361.1 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa60464
Clone ID Related Accession (Same CDS sequence)	XM_017591872.1
Accession Version	XM_017591872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAG GTAACTTCAT GGAATGTCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAGACT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACGA CTTCCACAGT GACCCTGGCC 
CCTGGACAGG AGAAGAGCTT GGCTGCTGAG GGCAGGGCTG ACACGACCAC TGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAAATCGCAG AGCACAGTCA CTCAGGCACC AGAGTGCTTC 
GATCCTGCAG GGCCAGCTGG TTTCGTGAGG CCGACCTCTG GCTTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTCATTGCT CTGGACTCTG AAAAACCGAA GAAACTTCGC 
TTCCACCCGA AGCAGCTGTA CTTCTCCGCT AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACACGCTG CTGCAGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG AGGACCCCCC TGATGGAGGC CGCAGAGAAC 
AACCACTTGG ACGCAGTGAA GTACCTCATC AAGGCCGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACGTGTTT GCACTTGGCT GCCAAGAAAG GCCACTATGA CGTGGTTCAG 
TATCTGCTGT CAAATGGACA GATGGATGTC AACTGCCAGG ATGATGGTGG TTGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GACCTGGTGA AGCTGCTGCT CTCTAAAGGC 
TCTGACATCA ATATCCGGGA CAATGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATCCTCCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCACGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT TTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GCCTCAATTC CCAGGTGTGG AGTGCACTGC AGATGAGCAA GGCACTTCAG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGAGA CATTGCCCGA 
GGATATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ATAGTGAGTT ATGTCCCACC 
AACTACAAGT ACGTCTCCCA GAACTGCGTG ACGTCCCCAA TGAACATCGA CAGGAACATA 
ACTCATTTGC AGTACTGCGT TTGTGTAGAC GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGACAAG GACGGGCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CCTGCTGGAG GAACTGCCGC 
AATCGCGTGG TGCAGAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT TCAGGATATC CCGCTGGGCA CCTTTGTCTG CGAATACGTG 
GGGGAGCTGA TTTCAGACTC CGAGGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTGTACTGC ATTGATGCTC GGTTCTACGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAGCCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTAC GGTTTCCCAG GATTGCCTTC TTCAGCACCC GCCTGATTCA GGCTGGGGAA 
CAGCTGGGGT TTGACTATGG GGAACGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTCCCAAGTG TCGGCACTCA AGTGCAGCCC TGGCCCAGCG GCAAGCCAGT 
GCAGCCCAGG AGGCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC TGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447361.1
CDS	21..3809
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_017591872.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591872.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAG GTAACTTCAT GGAATGTCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT 
AAGGACTGTG CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAGACT 
TCCAAGGCCA AAGAGGTGAC CATAGCAAAA GCAGACACGA CTTCCACAGT GACCCTGGCC 
CCTGGACAGG AGAAGAGCTT GGCTGCTGAG GGCAGGGCTG ACACGACCAC TGGCAGCATT 
GCTGGAGCCC CAGAGGATGA AAAATCGCAG AGCACAGTCA CTCAGGCACC AGAGTGCTTC 
GATCCTGCAG GGCCAGCTGG TTTCGTGAGG CCGACCTCTG GCTTTTCCCA GGGCCCAGGA 
AAGGAAACCT TGGAAAGTGC TCTCATTGCT CTGGACTCTG AAAAACCGAA GAAACTTCGC 
TTCCACCCGA AGCAGCTGTA CTTCTCCGCT AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC 
ATGCTGGTTG ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA 
TTACACGCTG CTGCAGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT 
GCCAATATTG ACACTTGCTC AGAGGACCAG AGGACCCCCC TGATGGAGGC CGCAGAGAAC 
AACCACTTGG ACGCAGTGAA GTACCTCATC AAGGCCGGAG CACAGGTGGA TCCGAAGGAC 
GCAGAGGGCT CCACGTGTTT GCACTTGGCT GCCAAGAAAG GCCACTATGA CGTGGTTCAG 
TATCTGCTGT CAAATGGACA GATGGATGTC AACTGCCAGG ATGATGGTGG TTGGACACCT 
ATGATCTGGG CCACTGAGTA CAAGCACGTG GACCTGGTGA AGCTGCTGCT CTCTAAAGGC 
TCTGACATCA ATATCCGGGA CAATGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA 
GGCTGTGTGG ACATAGCTGA AATCCTCCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT 
ATCCACGGAG ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC 
CTCTTTCTTT CTCGGGATTC AGATGTTACT TTGAAAAACA AGGAAGGAGA GACTCCCTTG 
CAGTGTGCAA GCCTCAATTC CCAGGTGTGG AGTGCACTGC AGATGAGCAA GGCACTTCAG 
GACTCAGCCC CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGAGA CATTGCCCGA 
GGATATGAGC GCATTCCCAT TCCCTGTGTC AATGCTGTGG ATAGTGAGTT ATGTCCCACC 
AACTACAAGT ACGTCTCCCA GAACTGCGTG ACGTCCCCAA TGAACATCGA CAGGAACATA 
ACTCATTTGC AGTACTGCGT TTGTGTAGAC GACTGCTCCT CTAGCACCTG CATGTGTGGC 
CAGCTGAGCA TGCGCTGCTG GTATGACAAG GACGGGCGAC TTCTGCCTGA GTTTAACATG 
GCAGAACCAC CCTTGATCTT CGAGTGCAAT CATGCCTGCT CCTGCTGGAG GAACTGCCGC 
AATCGCGTGG TGCAGAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG 
GGCTGGGGTG TGCGGTCCCT TCAGGATATC CCGCTGGGCA CCTTTGTCTG CGAATACGTG 
GGGGAGCTGA TTTCAGACTC CGAGGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT 
CTTGACAATA AGGATGGAGA GGTGTACTGC ATTGATGCTC GGTTCTACGG GAATGTCAGC 
CGGTTCATAA ACCACCACTG CGAGCCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC 
CAGGACCTAC GGTTTCCCAG GATTGCCTTC TTCAGCACCC GCCTGATTCA GGCTGGGGAA 
CAGCTGGGGT TTGACTATGG GGAACGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC 
CGGTGTGGGT CTCCCAAGTG TCGGCACTCA AGTGCAGCCC TGGCCCAGCG GCAAGCCAGT 
GCAGCCCAGG AGGCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC TGCTGCTGAC 
CCCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_017591873.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447362.1 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.

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CloneID	ORa60465
Clone ID Related Accession (Same CDS sequence)	XM_017591873.1
Accession Version	XM_017591873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTG TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGCTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAGC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TCACTCATTC CACTGTGGGT GCCAAGGGTG AGAAAGTCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCCG AGGAGGAAGA CTCTGAGGAG 
CTGGAGGATG AGGAGGACCG TGGCGCTGAG CAGGCTGCTG CATTTCCCAC TGAGGACAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GATCGTGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC TGACACAGGG GAGGACGAAG ACGGTGGAGA TGAGTCTGAC 
CTGAGTTCTG AATCTAGTAT CAAGAAGAAA TTTCTCAAGA GAAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGCA GAAAGAAGCC AAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCCCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGGT ATATGGAAGT TTCTCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CGGAAAACGA AGGGCTGGCC AATGGTCCGG ATGTGCTGGA GACGGATGGC 
CTCCATGAAG TGCCTCTCTG CAGCTGCCGC ATGGAAACCC CCAAGAGCCG TGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGTCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAGACTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACGAC TTCCACAGTG ACCCTGGCCC CTGGACAGGA GAAGAGCTTG 
GCTGCTGAGG GCAGGGCTGA CACGACCACT GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AAATCGCAGA GCACAGTCAC TCAGGCACCA GAGTGCTTCG ATCCTGCAGG GCCAGCTGGT 
TTCGTGAGGC CGACCTCTGG CTTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTCATTGCTC TGGACTCTGA AAAACCGAAG AAACTTCGCT TCCACCCGAA GCAGCTGTAC 
TTCTCCGCTA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACACGCTGC TGCAGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGA GGACCCCCCT GATGGAGGCC GCAGAGAACA ACCACTTGGA CGCAGTGAAG 
TACCTCATCA AGGCCGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACGTGTTTG 
CACTTGGCTG CCAAGAAAGG CCACTATGAC GTGGTTCAGT ATCTGCTGTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGATGGTGGT TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG ACCTGGTGAA GCTGCTGCTC TCTAAAGGCT CTGACATCAA TATCCGGGAC 
AATGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATCCTCCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCACGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTT TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG CCTCAATTCC 
CAGGTGTGGA GTGCACTGCA GATGAGCAAG GCACTTCAGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGAGAC ATTGCCCGAG GATATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA TAGTGAGTTA TGTCCCACCA ACTACAAGTA CGTCTCCCAG 
AACTGCGTGA CGTCCCCAAT GAACATCGAC AGGAACATAA CTCATTTGCA GTACTGCGTT 
TGTGTAGACG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGACAAGG ACGGGCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC CTGCTGGAGG AACTGCCGCA ATCGCGTGGT GCAGAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTT 
CAGGATATCC CGCTGGGCAC CTTTGTCTGC GAATACGTGG GGGAGCTGAT TTCAGACTCC 
GAGGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTGTACTGCA TTGATGCTCG GTTCTACGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAGCCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTACG GTTTCCCAGG 
ATTGCCTTCT TCAGCACCCG CCTGATTCAG GCTGGGGAAC AGCTGGGGTT TGACTATGGG 
GAACGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TCCCAAGTGT 
CGGCACTCAA GTGCAGCCCT GGCCCAGCGG CAAGCCAGTG CAGCCCAGGA GGCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCT GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447362.1
CDS	21..3788
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPDTSSAAAADPL

Target ORF information:

RefSeq Version	XM_017591873.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591873.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC AGCTGCAGTG TCTCGGAAGA AAAAACGAAG AATGGGAACC 
TATAGCTTAG TTCCCAAGAA AAAGACCAAA GTATTAAAGC AGAGGACGGT GATTGAGATG 
TTTAAAAGCA TCACTCATTC CACTGTGGGT GCCAAGGGTG AGAAAGTCTT AGATGATAGT 
GCCCTGCATG TAAATGGCGA GAGCTTGGAG ATGGACTCCG AGGAGGAAGA CTCTGAGGAG 
CTGGAGGATG AGGAGGACCG TGGCGCTGAG CAGGCTGCTG CATTTCCCAC TGAGGACAGT 
AGGACTTCTA AAGAGAGCAT GTCTGAGACT GATCGTGCTG CAAAGATGGA TGGAGATTCA 
GAGGAGGAAC AGGAGTCTCC TGACACAGGG GAGGACGAAG ACGGTGGAGA TGAGTCTGAC 
CTGAGTTCTG AATCTAGTAT CAAGAAGAAA TTTCTCAAGA GAAGAGGGAA GACTGACAGC 
CCCTGGATCA AACCTGCTCG GAAAAGGAGG CGGAGAAGCA GAAAGAAGCC AAGCAGCATG 
CTTGGTTCCG AGGCTTGTAA GTCATCCCCA GGAAGCATGG AGCAGGCAGC TCTGGGAGAC 
AGCGCTGGGT ATATGGAAGT TTCTCTGGAT TCCCTGGATC TCCGTGTCAG AGGAATTCTG 
TCTTCCCAGA CGGAAAACGA AGGGCTGGCC AATGGTCCGG ATGTGCTGGA GACGGATGGC 
CTCCATGAAG TGCCTCTCTG CAGCTGCCGC ATGGAAACCC CCAAGAGCCG TGAGATCAGC 
ACCCTGGCCA ACAACCAGTG CATGGCCACT GAGAGCGTGG ATCACGAAGG TAACTTCATG 
GAATGTCAGC CTGAGAGCAG CATCTCTCAT CGTTTCCATA AGGACTGTGC CTCTCGAGTC 
AACAATGCCA GCTACTGTCC CCATTGTGGG GAAGAGACTT CCAAGGCCAA AGAGGTGACC 
ATAGCAAAAG CAGACACGAC TTCCACAGTG ACCCTGGCCC CTGGACAGGA GAAGAGCTTG 
GCTGCTGAGG GCAGGGCTGA CACGACCACT GGCAGCATTG CTGGAGCCCC AGAGGATGAA 
AAATCGCAGA GCACAGTCAC TCAGGCACCA GAGTGCTTCG ATCCTGCAGG GCCAGCTGGT 
TTCGTGAGGC CGACCTCTGG CTTTTCCCAG GGCCCAGGAA AGGAAACCTT GGAAAGTGCT 
CTCATTGCTC TGGACTCTGA AAAACCGAAG AAACTTCGCT TCCACCCGAA GCAGCTGTAC 
TTCTCCGCTA GGCAGGGTGA GCTGCAGAAG GTGCTTCTCA TGCTGGTTGA TGGAATTGAT 
CCCAACTTCA AAATGGAGCA CCAAAGTAAG CGTTCCCCAT TACACGCTGC TGCAGAGGCT 
GGCCACGTGG ACATCTGCCA CATGCTGGTT CAGGCGGGTG CCAATATTGA CACTTGCTCA 
GAGGACCAGA GGACCCCCCT GATGGAGGCC GCAGAGAACA ACCACTTGGA CGCAGTGAAG 
TACCTCATCA AGGCCGGAGC ACAGGTGGAT CCGAAGGACG CAGAGGGCTC CACGTGTTTG 
CACTTGGCTG CCAAGAAAGG CCACTATGAC GTGGTTCAGT ATCTGCTGTC AAATGGACAG 
ATGGATGTCA ACTGCCAGGA TGATGGTGGT TGGACACCTA TGATCTGGGC CACTGAGTAC 
AAGCACGTGG ACCTGGTGAA GCTGCTGCTC TCTAAAGGCT CTGACATCAA TATCCGGGAC 
AATGAGGAGA ACATTTGTCT GCACTGGGCA GCATTTTCAG GCTGTGTGGA CATAGCTGAA 
ATCCTCCTGG CTGCCAAGTG TGACCTGCAT GCTGTGAATA TCCACGGAGA CTCACCCCTG 
CACATCGCAG CCAGGGAGAA TCGCTACGAC TGTGTTGTCC TCTTTCTTTC TCGGGATTCA 
GATGTTACTT TGAAAAACAA GGAAGGAGAG ACTCCCTTGC AGTGTGCAAG CCTCAATTCC 
CAGGTGTGGA GTGCACTGCA GATGAGCAAG GCACTTCAGG ACTCAGCCCC TGACAAGCCC 
GTTGCTGTTG AGAAGACGGT GAGCAGAGAC ATTGCCCGAG GATATGAGCG CATTCCCATT 
CCCTGTGTCA ATGCTGTGGA TAGTGAGTTA TGTCCCACCA ACTACAAGTA CGTCTCCCAG 
AACTGCGTGA CGTCCCCAAT GAACATCGAC AGGAACATAA CTCATTTGCA GTACTGCGTT 
TGTGTAGACG ACTGCTCCTC TAGCACCTGC ATGTGTGGCC AGCTGAGCAT GCGCTGCTGG 
TATGACAAGG ACGGGCGACT TCTGCCTGAG TTTAACATGG CAGAACCACC CTTGATCTTC 
GAGTGCAATC ATGCCTGCTC CTGCTGGAGG AACTGCCGCA ATCGCGTGGT GCAGAATGGT 
CTCAGGGCAA GGCTGCAGCT TTATCGGACA CAGGACATGG GCTGGGGTGT GCGGTCCCTT 
CAGGATATCC CGCTGGGCAC CTTTGTCTGC GAATACGTGG GGGAGCTGAT TTCAGACTCC 
GAGGCTGATG TTCGGGAAGA GGACTCTTAC CTCTTTGATC TTGACAATAA GGATGGAGAG 
GTGTACTGCA TTGATGCTCG GTTCTACGGG AATGTCAGCC GGTTCATAAA CCACCACTGC 
GAGCCCAACC TTGTGCCTGT GCGAGTGTTC ATGTCACACC AGGACCTACG GTTTCCCAGG 
ATTGCCTTCT TCAGCACCCG CCTGATTCAG GCTGGGGAAC AGCTGGGGTT TGACTATGGG 
GAACGCTTTT GGGACGTCAA GGGCAAGCTC TTCAGTTGCC GGTGTGGGTC TCCCAAGTGT 
CGGCACTCAA GTGCAGCCCT GGCCCAGCGG CAAGCCAGTG CAGCCCAGGA GGCTCAGGAG 
AATGGCCTTC CAGATACCAG CTCTGCAGCT GCTGCTGACC CCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt1 ( XM_017591874.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447363.1 Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

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CloneID	ORa60466
Clone ID Related Accession (Same CDS sequence)	XM_017591874.1
Accession Version	XM_017591874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447363.1
CDS	21..2774
Translation	MARGRGGATLRARAMAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNL

Target ORF information:

RefSeq Version	XM_017591874.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 1 (Ehmt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591874.1

ORF Insert Sequence:

ATGGCGCGCG GGAGGGGCGG GGCCACGCTG CGGGCCCGGG CCATGGCCGC CGCGGATGCT 
GAGCAGGCAG TTCTGGCCAA GCAAGAGACC AAGCAGGATT GCTGCATGAA AACTGAACTG 
CTAAGGGAAG ATACACTTAT GGCTGCTGAT GAAGGTTCCA CAGAGAAGCA AGCAGGAGAG 
ACTCCTATGG CTGCAGATGG AGAAACCAAT GGGTCTTGTG AAAAGAGTGG CGATATCAGC 
CATCCAAATG CACCCAAACA TACTCAGGAG AACACAAGAG CTAGCCCACA GGAAGGCACC 
AACAGAGTAT CTCGGGTGGC GGAAAATGGG GTTTCAGAAA GAGACACAGA AGTGGGGAAG 
CAAAACCATG TTACAGCTGA CGACTTCATG CAGACGTCTG TCATTGGAAG CAATGGATAT 
TTCTTAAATA AACCAGCCCT GCAGGGACAG CCCTTGAGGA CTCCCAACAC TCTAAATTCC 
TCGCTTCCTG GTCATGCCGC AAAAACGCTT CCTGGAGGAG CCAGTAAGTG CAGGACTCCA 
AGTGCACTTC CTCAGACACC AACCACAGCA CCCACTGTGC CTGGGGAAGG GAGTGCAGAC 
ACAGAGGACA GAAAGCCTAC AGCCTCGGGC ACTGATGTCA GGGTTCACAG GGCACGCAAG 
ACCATGCCGA AGTCCATCCT GGGCCTGCAC GCAGCCAGTA AAGACCATAG AGAAGTTCAA 
GACCATAAGG AACCAAAAGA GGATATCAAC AGAAACATTT CTGAATGTGG ACGACAGCAG 
CTTTTACCAA CTTTCCCAGC CCTCCACCAG TCGCTACCTC AGAATCAATG CTACATGGCC 
ACCACAAAGT CCCAGACAGC TTGCTTGCCT TTTGTTTTAG CAGCTGCAGT GTCTCGGAAG 
AAAAAACGAA GAATGGGAAC CTATAGCTTA GTTCCCAAGA AAAAGACCAA AGTATTAAAG 
CAGAGGACGG TGATTGAGAT GTTTAAAAGC ATCACTCATT CCACTGTGGG TGCCAAGGGT 
GAGAAAGTCT TAGATGATAG TGCCCTGCAT GTAAATGGCG AGAGCTTGGA GATGGACTCC 
GAGGAGGAAG ACTCTGAGGA GCTGGAGGAT GAGGAGGACC GTGGCGCTGA GCAGGCTGCT 
GCATTTCCCA CTGAGGACAG TAGGACTTCT AAAGAGAGCA TGTCTGAGAC TGATCGTGCT 
GCAAAGATGG ATGGAGATTC AGAGGAGGAA CAGGAGTCTC CTGACACAGG GGAGGACGAA 
GACGGTGGAG ATGAGTCTGA CCTGAGTTCT GAATCTAGTA TCAAGAAGAA ATTTCTCAAG 
AGAAGAGGGA AGACTGACAG CCCCTGGATC AAACCTGCTC GGAAAAGGAG GCGGAGAAGC 
AGAAAGAAGC CAAGCAGCAT GCTTGGTTCC GAGGCTTGTA AGTCATCCCC AGGAAGCATG 
GAGCAGGCAG CTCTGGGAGA CAGCGCTGGG TATATGGAAG TTTCTCTGGA TTCCCTGGAT 
CTCCGTGTCA GAGGAATTCT GTCTTCCCAG ACGGAAAACG AAGGGCTGGC CAATGGTCCG 
GATGTGCTGG AGACGGATGG CCTCCATGAA GTGCCTCTCT GCAGCTGCCG CATGGAAACC 
CCCAAGAGCC GTGAGATCAG CACCCTGGCC AACAACCAGT GCATGGCCAC TGAGAGCGTG 
GATCACGAAT TGGGCCGGTG TACAAACAGC GTGGTCAAGT ATGAGCTGAT GCGCCCTTCC 
AACAAAGCAC CACTCTTGGT GCTGTGTGAA GACCATCGGG GTCGCATGGT GAAGCACCAG 
TGCTGTCCTG GCTGCGGCTA CTTCTGCACG GCGGGTAACT TCATGGAATG TCAGCCTGAG 
AGCAGCATCT CTCATCGTTT CCATAAGGAC TGTGCCTCTC GAGTCAACAA TGCCAGCTAC 
TGTCCCCATT GTGGGGAAGA GACTTCCAAG GCCAAAGAGG TGACCATAGC AAAAGCAGAC 
ACGACTTCCA CAGTGACCCT GGCCCCTGGA CAGGAGAAGA GCTTGGCTGC TGAGGGCAGG 
GCTGACACGA CCACTGGCAG CATTGCTGGA GCCCCAGAGG ATGAAAAATC GCAGAGCACA 
GTCACTCAGG CACCAGAGTG CTTCGATCCT GCAGGGCCAG CTGGTTTCGT GAGGCCGACC 
TCTGGCTTTT CCCAGGGCCC AGGAAAGGAA ACCTTGGAAA GTGCTCTCAT TGCTCTGGAC 
TCTGAAAAAC CGAAGAAACT TCGCTTCCAC CCGAAGCAGC TGTACTTCTC CGCTAGGCAG 
GGTGAGCTGC AGAAGGTGCT TCTCATGCTG GTTGATGGAA TTGATCCCAA CTTCAAAATG 
GAGCACCAAA GTAAGCGTTC CCCATTACAC GCTGCTGCAG AGGCTGGCCA CGTGGACATC 
TGCCACATGC TGGTTCAGGC GGGTGCCAAT ATTGACACTT GCTCAGAGGA CCAGAGGACC 
CCCCTGATGG AGGCCGCAGA GAACAACCAC TTGGACGCAG TGAAGTACCT CATCAAGGCC 
GGAGCACAGG TGGATCCGAA GGACGCAGAG GGCTCCACGT GTTTGCACTT GGCTGCCAAG 
AAAGGCCACT ATGACGTGGT TCAGTATCTG CTGTCAAATG GACAGATGGA TGTCAACTGC 
CAGGATGATG GTGGTTGGAC ACCTATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTG 
GTGAAGCTGC TGCTCTCTAA AGGCTCTGAC ATCAATATCC GGGACAATTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
Aging cell16(5)1062-1072(2017 Oct)
Sharma M,Dierkes T,Sajikumar S

The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.
The Journal of clinical investigation127(1)335-348(2017 Jan)
Thienpont B,Aronsen JM,Robinson EL,Okkenhaug H,Loche E,Ferrini A,Brien P,Alkass K,Tomasso A,Agrawal A,Bergmann O,Sjaastad I,Reik W,Roderick HL

Overexpression of G9a and MCM7 in oesophageal squamous cell carcinoma is associated with poor prognosis.
Histopathology66(2)192-200(2015 Jan)
Zhong X,Chen X,Guan X,Zhang H,Ma Y,Zhang S,Wang E,Zhang L,Han Y

Analysis of EHMT1 expression and its correlations with clinical significance in esophageal squamous cell cancer.
Molecular and clinical oncology2(1)76-80(2014 Jan)
Guan X,Zhong X,Men W,Gong S,Zhang L,Han Y

EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex.
Nature504(7478)163-7(2013 Dec)
Ohno H,Shinoda K,Ohyama K,Sharp LZ,Kajimura S

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Ehmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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