gRNAs
The following Cgn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cgn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu32272 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006502064.1 | |
| Accession Version | XM_006502064.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006502127.1 |
| CDS | 164..2374 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006502064.1 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23510 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006502062.3 , XM_006502061.2 , NM_001037711.2 , XM_006502062.4 | |
| Accession Version | NM_001037711.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-19 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158989.1 and AC150033.2. On Jun 6, 2008 this sequence version replaced NM_001037711.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173148.1, BC042459.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001032800.2 |
| CDS | 170..3748 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001037711.2 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu84943 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001293727.2 | |
| Accession Version | NM_001293727.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2020-01-01 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158989.1 and AC150033.2. On Apr 13, 2019 this sequence version replaced NM_001293727.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064474.1, SRR7345562.1893608.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGATC CCCGGGGCCC CGTAGACCAT GGAGTTCAAA TTCGATTTAT TACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001280656.2 |
| CDS | 140..3691 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001293727.2 |
1 | ATGGCTGATC CCCGGGGCCC CGTAGACCAT GGAGTTCAAA TTCGATTTAT TACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu84946 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001037711.3 | |
| Accession Version | NM_001037711.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2020-01-01 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158989.1 and AC150033.2. On Apr 13, 2019 this sequence version replaced NM_001037711.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173148.1, BC042459.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGATC CCCGGGGCCC CGTAGACCAT GGAGTTCAAA TTCGATTTAT TACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001032800.3 |
| CDS | 140..3715 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001037711.3 |
1 | ATGGCTGATC CCCGGGGCCC CGTAGACCAT GGAGTTCAAA TTCGATTTAT TACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23510 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006502062.3 , XM_006502061.2 , NM_001037711.2 , XM_006502062.4 | |
| Accession Version | XM_006502062.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006502125.1 |
| CDS | 114..3692 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006502062.3 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23510 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006502062.3 , XM_006502061.2 , NM_001037711.2 , XM_006502062.4 | |
| Accession Version | XM_006502061.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006502124.1 |
| CDS | 128..3706 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006502061.2 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23510 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006502062.3 , XM_006502061.2 , NM_001037711.2 , XM_006502062.4 | |
| Accession Version | XM_006502062.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006502125.1 |
| CDS | 92..3670 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006502062.4 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23519 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001293727.1 , XM_030252817.1 | |
| Accession Version | XM_030252817.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030108677.1 |
| CDS | 128..3682 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030252817.1 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23519 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001293727.1 , XM_030252817.1 | |
| Accession Version | NM_001293727.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-23 | |
| Organism | Mus musculus(house mouse) | |
| Product | cingulin isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158989.1 and AC150033.2. On Jun 17, 2014 this sequence version replaced XM_006502063.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064474.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001280656.1 |
| CDS | 170..3724 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001293727.1 |
1 | ATGATGGCTG ATCCCCGGGG CCCCGTAGAC CATGGAGTTC AAATTCGATT TATTACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Pluripotent state transitions coordinate morphogenesis in mouse and human embryos. |
High-throughput discovery of novel developmental phenotypes. |
Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo. |
The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK. |
