gRNAs
The following CGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OCe00239 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001103217.1 | |
| Accession Version | NM_001103217.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | cingulin | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ910799.1. On or before Sep 13, 2007 this sequence version replaced XM_540316.2, XM_856969.1. ##Evidence-Data-START## Transcript exon combination :: DQ910799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCTGAGC CCCGGGGCCC TGTAGACCAT GGAGTCCAGA TTCGCTTCAT CACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001096687.1 |
| CDS | 29..3601 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001103217.1 |
1 | ATGGCTGAGC CCCGGGGCCC TGTAGACCAT GGAGTCCAGA TTCGCTTCAT CACAGAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630616.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | cingulin isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630673.1 |
| CDS | 129..3719 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630616.2 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_014120562.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | cingulin isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_013976037.1 |
| CDS | 164..3754 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_014120562.1 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630616.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630673.1 |
| CDS | 119..3709 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630616.3 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630618.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630675.1 |
| CDS | 197..3787 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630618.3 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630618.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | cingulin isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630675.1 |
| CDS | 217..3807 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630618.2 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_014120562.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_013976037.1 |
| CDS | 136..3726 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_014120562.2 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630617.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | cingulin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630674.1 |
| CDS | 65..3655 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630617.3 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe19270 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630616.2 , XM_014120562.1 , XM_005630616.3 , XM_005630618.3 , XM_005630618.2 , XM_014120562.2 , XM_005630617.3 , XM_005630617.2 | |
| Accession Version | XM_005630617.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | cingulin isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630674.1 |
| CDS | 58..3648 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630617.2 |
1 | ATGGAGCAGG CATCCACCAT GGCTGAGCCC CGGGGCCCTG TAGACCATGG AGTCCAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells. |
Cingulin and paracingulin show similar dynamic behaviour, but are recruited independently to junctions. |
Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression. |
Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. |
