cgnb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cgnb
Entrez Gene ID	100005752
Full Name	cingulin b
Synonyms	cb117,cgn,im:7137721,sb:cb117,sb:cb117.,si:dkey-204a24.2
General protein information	Preferred Names cingulin b Names cingulin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159385.3	XP_005159442.1	cingulin isoform X1
XM_005159386.3	XP_005159443.1	cingulin isoform X2
XM_005159386.4	XP_005159443.1	cingulin isoform X2
NM_001044762.1	NP_001038227.1	cingulin
XM_005159385.4	XP_005159442.1	cingulin isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cgn	NP_001032800.2
Gallus gallus (chicken)	CGN	XP_004948438.1
Danio rerio (zebrafish)	LOC561423	XP_689920.3
Pan troglodytes (chimpanzee)	CGN	XP_513800.2
Danio rerio (zebrafish)	cgnb	NP_001038227.1
Bos taurus (cattle)	CGN	NP_001179715.1
	LOC710830	XP_001108356.1
Xenopus tropicalis (tropical clawed frog)	cgn	NP_001120598.1
Homo sapiens (human)	CGN	NP_065821.1
Canis lupus familiaris (dog)	CGN	NP_001096687.1
Rattus norvegicus (Norway rat)	Cgn	XP_006232990.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cgnb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cgnb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00003	NM_001044762.1 Latest version!	Danio rerio cingulin b (cgnb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa23843	XM_005159385.4 Latest version!	Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23844	XM_005159386.4 Latest version!	Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa23844	XM_005159386.3	Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa23843	XM_005159385.3	Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cgnb ( NM_001044762.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038227.1 Danio rerio cingulin b (cgnb), mRNA.

Price & Availability↑

CloneID	ODa00003
Clone ID Related Accession (Same CDS sequence)	NM_001044762.1
Accession Version	NM_001044762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	cingulin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR628394.17. On Sep 1, 2006 this sequence version replaced XM_686569.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTGAGGAAG 
ATTCAGCAGT CTCGACGGTC GACTCTGGGA AGCACATTAA GCTCTGACGA GGAGGACAAT 
TATTCAGACA CCAAGAGCAT CACATCTATT CTCACCGATT CACCACTTCA GACCACCAGC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038227.1
CDS	138..3623
Translation	MSSLSADRKPPLDYGVQIRFIKDLDDAGGGFPDKSRLANGVGGVSNGTSSPSKYGVAVRVQGISGQPYVVLQDGEKGDSYGVQLKTQPQIQSAPPVVSQTSPYNTLLLGQREGARTPQGSYNPTDQPSSPDEDFGSPLRRPPGDGQAGTQGEAEPRTTERFTPSALAPKLDKKDNDAFNEAGLRKVKQNGIGSSLNGTGLNGSFPPPSSAQDEDQAPAIDTKSLAPINKLISRFGGGGGGSGDILNSEAQTRPRLDNRVRSQSADALNKPSEEASSTSPTINPYAPNTSATVPKLNSTKPSSTGSLGRDATSVAKVAAIPNFKPLAFNQSPKLFAPKEAPPAVPKKPVTPDLIKSQTTSIENGNGEDDQTKQAIYNILKEGSIEKEEAIKRKASLIHERFCGVKAPQISVTDSNMKTELEQAFGRNTQLQQQLDKSRRELQENQDQMVELRMDREGAESRLRQQEDQLAQLQEELRRTLENSPQSDSMQLDLLTVQAELSESQLLRQKLEDTLRQRERELTALKGALKDEVASHDKEMEALREQFSQDMDALRHSMETVSQSQLEIEEERQKVNASILAMEEELEGYKEQSEQWKKQFSSANQELLKAQQGKRELEEKLLAVVKQTDETDSNSVMKELQQCRDSLKKAQSELEKQKAETLKKQEELKSATRASEKRETELKAEIDRLINQLKKEKEELSKAIEKTQQPLVSDQTKDPESNLELQEANARLRERIARMTRLHSSVPDSSSSDALEEENRSLKTQLEESRRAASRLGVEKEELNRRLEEREREREALRRGKSDLEEQKRLLDRSLDKINKEMESMMGDSRQSVQVLQSQLEEFRDRSRRELQDAQRLSKDRLVELQRAQALLKTTQEEVSRVKKELLSCTEERDSTQLDKELLSSRLKNMETELQTDRSSQTDRSREIRLLEDKVKTLEIELDEEKSGAELLNERITRCREQVDQLRSELMQERSARHDLEMDKSALERQIKELKSRIADMGTQSRPSAGVTMLENKVQELEDRLRSEEREKNTIQAAQRRLDRKLKDVTATLDQERNQHAEQRDQLSLRVKALKRQLDESEGEVERLEGVRRKVLRELEEQRELQAALQAKVNAMDNELRKIQQSRRSTLGSTLSSDEEDNYSDTKSITSILTDSPLQTTSC

Target ORF information:

RefSeq Version	NM_001044762.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin b (cgnb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044762.1

ORF Insert Sequence:

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTGAGGAAG 
ATTCAGCAGT CTCGACGGTC GACTCTGGGA AGCACATTAA GCTCTGACGA GGAGGACAAT 
TATTCAGACA CCAAGAGCAT CACATCTATT CTCACCGATT CACCACTTCA GACCACCAGC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnb ( XM_005159385.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159442.1 Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23843
Clone ID Related Accession (Same CDS sequence)	XM_005159385.3 , XM_005159385.4
Accession Version	XM_005159385.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cingulin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AACGAGGGCA GTACAGCGCG TTTCAAAAGC TTCTGAAGGC TCAGCAGGGC 
AAGAGGGAAC TAGAAGAGAA GCTGCTTGCA GTTGTAAAAC AGACCGATGA AACTGATTCA 
AACTCAGTGA TGAAAGAGTT GCAGCAGTGC CGAGATAGTC TGAAAAAAGC TCAATCAGAA 
CTGGAAAAAC AAAAAGCAGA GACGCTGAAA AAACAGGAGG AGCTTAAATC AGCTACGAGA 
GCCAGTGAGA AGCGGGAAAC TGAGCTGAAG GCAGAAATCG ACAGACTAAT AAATCAGTTA 
AAGAAAGAAA AAGAGGAGCT TTCAAAAGCA ATAGAGAAAA CACAACAGCC TTTAGTATCA 
GATCAAACCA AGGATCCTGA AAGCAATCTA GAACTTCAAG AGGCCAATGC TCGCTTGAGG 
GAGAGAATAG CACGCATGAC GCGGCTGCAC TCGAGTGTTC CTGACAGCAG CTCGTCTGAT 
GCTCTGGAGG AGGAGAACCG CAGCCTGAAG ACTCAGCTGG AGGAGTCGCG GCGCGCAGCC 
TCTCGACTGG GTGTGGAGAA AGAGGAGCTG AACCGACGGC TGGAGGAGCG TGAGAGAGAG 
AGGGAAGCTC TGAGACGCGG CAAGAGCGAC CTGGAGGAGC AGAAGAGACT GCTGGACCGA 
TCCCTGGACA AGATCAACAA GGAGATGGAG TCTATGATGG GAGACTCTCG TCAGTCGGTG 
CAGGTTCTTC AGTCTCAGCT GGAAGAATTC AGGGATCGTT CTCGCAGAGA GCTGCAGGAC 
GCTCAAAGAC TCAGTAAAGA CAGACTGGTT GAGCTGCAGA GAGCGCAGGC CCTCCTCAAA 
ACCACCCAGG AGGAGGTGTC TCGTGTGAAG AAGGAGCTGC TGTCATGCAC AGAGGAGAGA 
GACAGCACCC AGCTGGATAA AGAGCTCCTG AGCAGCCGAC TTAAAAACAT GGAGACAGAG 
CTGCAGACAG ACAGAAGCTC ACAGACGGAC CGCAGCAGAG AGATTCGCCT CTTAGAGGAC 
AAAGTGAAGA CATTAGAGAT CGAGCTGGAC GAGGAGAAGA GCGGAGCGGA GCTACTGAAC 
GAACGCATCA CTCGCTGTCG AGAACAGGTG GATCAGCTTC GATCTGAACT GATGCAGGAG 
CGTTCAGCCA GACATGATCT TGAGATGGAT AAAAGTGCGC TGGAGAGACA GATTAAGGAG 
TTGAAGTCTC GTATTGCTGA TATGGGCACT CAGTCTCGTC CCTCTGCTGG AGTCACAATG 
CTGGAAAACA AGGTTCAGGA GCTGGAGGAC CGTCTGCGCA GTGAAGAAAG AGAGAAAAAC 
ACCATTCAGG CAGCGCAGAG GAGATTGGAC AGGAAACTAA AGGATGTGAC GGCCACCCTT 
GACCAGGAGC GAAATCAACA CGCTGAACAG AGAGACCAGC TGTCTCTGCG AGTGAAGGCT 
CTGAAGCGTC AGCTGGATGA GAGCGAGGGT GAGGTGGAGC GTCTGGAGGG TGTCAGACGG 
AAGGTTCTGC GGGAGCTCGA GGAGCAGCGG GAGCTTCAGG CGGCGCTGCA GGCCAAAGTC 
AACGCAATGG ACAACGAGCT CAAGAGGAAG ATTCAGCAGT CTCGACGGTC GACTCTGGGA 
AGCACATTAA GCTCTGACGA GGAGGACAAT TATTCAGACA CCAAGAGCAT CACATCTATT 
CTCACCGATT CACCACTTCA GACCACCAGC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159442.1
CDS	210..3725
Translation	MSSLSADRKPPLDYGVQIRFIKDLDDAGGGFPDKSRLANGVGGVSNGTSSPSKYGVAVRVQGISGQPYVVLQDGEKGDSYGVQLKTQPQIQSAPPVVSQTSPYNTLLLGQREGARTPQGSYNPTDQPSSPDEDFGSPLRRPPGDGQAGTQGEAEPRTTERFTPSALAPKLDKKDNDAFNEAGLRKVKQNGIGSSLNGTGLNGSFPPPSSAQDEDQAPAIDTKSLAPINKLISRFGGGGGGSGDILNSEAQTRPRLDNRVRSQSADALNKPSEEASSTSPTINPYAPNTSATVPKLNSTKPSSTGSLGRDATSVAKVAAIPNFKPLAFNQSPKLFAPKEAPPAVPKKPVTPDLIKSQTTSIENGNGEDDQTKQAIYNILKEGSIEKEEAIKRKASLIHERFCGVKAPQISVTDSNMKTELEQAFGRNTQLQQQLDKSRRELQENQDQMVELRMDREGAESRLRQQEDQLAQLQEELRRTLENSPQSDSMQLDLLTVQAELSESQLLRQKLEDTLRQRERELTALKGALKDEVASHDKEMEALREQFSQDMDALRHSMETVSQSQLEIEEERQKVNASILAMEEELEGYKEQSEQWKKQFSSANQERGQYSAFQKLLKAQQGKRELEEKLLAVVKQTDETDSNSVMKELQQCRDSLKKAQSELEKQKAETLKKQEELKSATRASEKRETELKAEIDRLINQLKKEKEELSKAIEKTQQPLVSDQTKDPESNLELQEANARLRERIARMTRLHSSVPDSSSSDALEEENRSLKTQLEESRRAASRLGVEKEELNRRLEEREREREALRRGKSDLEEQKRLLDRSLDKINKEMESMMGDSRQSVQVLQSQLEEFRDRSRRELQDAQRLSKDRLVELQRAQALLKTTQEEVSRVKKELLSCTEERDSTQLDKELLSSRLKNMETELQTDRSSQTDRSREIRLLEDKVKTLEIELDEEKSGAELLNERITRCREQVDQLRSELMQERSARHDLEMDKSALERQIKELKSRIADMGTQSRPSAGVTMLENKVQELEDRLRSEEREKNTIQAAQRRLDRKLKDVTATLDQERNQHAEQRDQLSLRVKALKRQLDESEGEVERLEGVRRKVLRELEEQRELQAALQAKVNAMDNELKRKIQQSRRSTLGSTLSSDEEDNYSDTKSITSILTDSPLQTTSC

Target ORF information:

RefSeq Version	XM_005159385.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159385.3

ORF Insert Sequence:

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AACGAGGGCA GTACAGCGCG TTTCAAAAGC TTCTGAAGGC TCAGCAGGGC 
AAGAGGGAAC TAGAAGAGAA GCTGCTTGCA GTTGTAAAAC AGACCGATGA AACTGATTCA 
AACTCAGTGA TGAAAGAGTT GCAGCAGTGC CGAGATAGTC TGAAAAAAGC TCAATCAGAA 
CTGGAAAAAC AAAAAGCAGA GACGCTGAAA AAACAGGAGG AGCTTAAATC AGCTACGAGA 
GCCAGTGAGA AGCGGGAAAC TGAGCTGAAG GCAGAAATCG ACAGACTAAT AAATCAGTTA 
AAGAAAGAAA AAGAGGAGCT TTCAAAAGCA ATAGAGAAAA CACAACAGCC TTTAGTATCA 
GATCAAACCA AGGATCCTGA AAGCAATCTA GAACTTCAAG AGGCCAATGC TCGCTTGAGG 
GAGAGAATAG CACGCATGAC GCGGCTGCAC TCGAGTGTTC CTGACAGCAG CTCGTCTGAT 
GCTCTGGAGG AGGAGAACCG CAGCCTGAAG ACTCAGCTGG AGGAGTCGCG GCGCGCAGCC 
TCTCGACTGG GTGTGGAGAA AGAGGAGCTG AACCGACGGC TGGAGGAGCG TGAGAGAGAG 
AGGGAAGCTC TGAGACGCGG CAAGAGCGAC CTGGAGGAGC AGAAGAGACT GCTGGACCGA 
TCCCTGGACA AGATCAACAA GGAGATGGAG TCTATGATGG GAGACTCTCG TCAGTCGGTG 
CAGGTTCTTC AGTCTCAGCT GGAAGAATTC AGGGATCGTT CTCGCAGAGA GCTGCAGGAC 
GCTCAAAGAC TCAGTAAAGA CAGACTGGTT GAGCTGCAGA GAGCGCAGGC CCTCCTCAAA 
ACCACCCAGG AGGAGGTGTC TCGTGTGAAG AAGGAGCTGC TGTCATGCAC AGAGGAGAGA 
GACAGCACCC AGCTGGATAA AGAGCTCCTG AGCAGCCGAC TTAAAAACAT GGAGACAGAG 
CTGCAGACAG ACAGAAGCTC ACAGACGGAC CGCAGCAGAG AGATTCGCCT CTTAGAGGAC 
AAAGTGAAGA CATTAGAGAT CGAGCTGGAC GAGGAGAAGA GCGGAGCGGA GCTACTGAAC 
GAACGCATCA CTCGCTGTCG AGAACAGGTG GATCAGCTTC GATCTGAACT GATGCAGGAG 
CGTTCAGCCA GACATGATCT TGAGATGGAT AAAAGTGCGC TGGAGAGACA GATTAAGGAG 
TTGAAGTCTC GTATTGCTGA TATGGGCACT CAGTCTCGTC CCTCTGCTGG AGTCACAATG 
CTGGAAAACA AGGTTCAGGA GCTGGAGGAC CGTCTGCGCA GTGAAGAAAG AGAGAAAAAC 
ACCATTCAGG CAGCGCAGAG GAGATTGGAC AGGAAACTAA AGGATGTGAC GGCCACCCTT 
GACCAGGAGC GAAATCAACA CGCTGAACAG AGAGACCAGC TGTCTCTGCG AGTGAAGGCT 
CTGAAGCGTC AGCTGGATGA GAGCGAGGGT GAGGTGGAGC GTCTGGAGGG TGTCAGACGG 
AAGGTTCTGC GGGAGCTCGA GGAGCAGCGG GAGCTTCAGG CGGCGCTGCA GGCCAAAGTC 
AACGCAATGG ACAACGAGCT CAAGAGGAAG ATTCAGCAGT CTCGACGGTC GACTCTGGGA 
AGCACATTAA GCTCTGACGA GGAGGACAAT TATTCAGACA CCAAGAGCAT CACATCTATT 
CTCACCGATT CACCACTTCA GACCACCAGC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnb ( XM_005159385.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159442.1 Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.

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CloneID	ODa23843
Clone ID Related Accession (Same CDS sequence)	XM_005159385.3 , XM_005159385.4
Accession Version	XM_005159385.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159385.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AACGAGGGCA GTACAGCGCG TTTCAAAAGC TTCTGAAGGC TCAGCAGGGC 
AAGAGGGAAC TAGAAGAGAA GCTGCTTGCA GTTGTAAAAC AGACCGATGA AACTGATTCA 
AACTCAGTGA TGAAAGAGTT GCAGCAGTGC CGAGATAGTC TGAAAAAAGC TCAATCAGAA 
CTGGAAAAAC AAAAAGCAGA GACGCTGAAA AAACAGGAGG AGCTTAAATC AGCTACGAGA 
GCCAGTGAGA AGCGGGAAAC TGAGCTGAAG GCAGAAATCG ACAGACTAAT AAATCAGTTA 
AAGAAAGAAA AAGAGGAGCT TTCAAAAGCA ATAGAGAAAA CACAACAGCC TTTAGTATCA 
GATCAAACCA AGGATCCTGA AAGCAATCTA GAACTTCAAG AGGCCAATGC TCGCTTGAGG 
GAGAGAATAG CACGCATGAC GCGGCTGCAC TCGAGTGTTC CTGACAGCAG CTCGTCTGAT 
GCTCTGGAGG AGGAGAACCG CAGCCTGAAG ACTCAGCTGG AGGAGTCGCG GCGCGCAGCC 
TCTCGACTGG GTGTGGAGAA AGAGGAGCTG AACCGACGGC TGGAGGAGCG TGAGAGAGAG 
AGGGAAGCTC TGAGACGCGG CAAGAGCGAC CTGGAGGAGC AGAAGAGACT GCTGGACCGA 
TCCCTGGACA AGATCAACAA GGAGATGGAG TCTATGATGG GAGACTCTCG TCAGTCGGTG 
CAGGTTCTTC AGTCTCAGCT GGAAGAATTC AGGGATCGTT CTCGCAGAGA GCTGCAGGAC 
GCTCAAAGAC TCAGTAAAGA CAGACTGGTT GAGCTGCAGA GAGCGCAGGC CCTCCTCAAA 
ACCACCCAGG AGGAGGTGTC TCGTGTGAAG AAGGAGCTGC TGTCATGCAC AGAGGAGAGA 
GACAGCACCC AGCTGGATAA AGAGCTCCTG AGCAGCCGAC TTAAAAACAT GGAGACAGAG 
CTGCAGACAG ACAGAAGCTC ACAGACGGAC CGCAGCAGAG AGATTCGCCT CTTAGAGGAC 
AAAGTGAAGA CATTAGAGAT CGAGCTGGAC GAGGAGAAGA GCGGAGCGGA GCTACTGAAC 
GAACGCATCA CTCGCTGTCG AGAACAGGTG GATCAGCTTC GATCTGAACT GATGCAGGAG 
CGTTCAGCCA GACATGATCT TGAGATGGAT AAAAGTGCGC TGGAGAGACA GATTAAGGAG 
TTGAAGTCTC GTATTGCTGA TATGGGCACT CAGTCTCGTC CCTCTGCTGG AGTCACAATG 
CTGGAAAACA AGGTTCAGGA GCTGGAGGAC CGTCTGCGCA GTGAAGAAAG AGAGAAAAAC 
ACCATTCAGG CAGCGCAGAG GAGATTGGAC AGGAAACTAA AGGATGTGAC GGCCACCCTT 
GACCAGGAGC GAAATCAACA CGCTGAACAG AGAGACCAGC TGTCTCTGCG AGTGAAGGCT 
CTGAAGCGTC AGCTGGATGA GAGCGAGGGT GAGGTGGAGC GTCTGGAGGG TGTCAGACGG 
AAGGTTCTGC GGGAGCTCGA GGAGCAGCGG GAGCTTCAGG CGGCGCTGCA GGCCAAAGTC 
AACGCAATGG ACAACGAGCT CAAGAGGAAG ATTCAGCAGT CTCGACGGTC GACTCTGGGA 
AGCACATTAA GCTCTGACGA GGAGGACAAT TATTCAGACA CCAAGAGCAT CACATCTATT 
CTCACCGATT CACCACTTCA GACCACCAGC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159442.1
CDS	309..3824
Translation	MSSLSADRKPPLDYGVQIRFIKDLDDAGGGFPDKSRLANGVGGVSNGTSSPSKYGVAVRVQGISGQPYVVLQDGEKGDSYGVQLKTQPQIQSAPPVVSQTSPYNTLLLGQREGARTPQGSYNPTDQPSSPDEDFGSPLRRPPGDGQAGTQGEAEPRTTERFTPSALAPKLDKKDNDAFNEAGLRKVKQNGIGSSLNGTGLNGSFPPPSSAQDEDQAPAIDTKSLAPINKLISRFGGGGGGSGDILNSEAQTRPRLDNRVRSQSADALNKPSEEASSTSPTINPYAPNTSATVPKLNSTKPSSTGSLGRDATSVAKVAAIPNFKPLAFNQSPKLFAPKEAPPAVPKKPVTPDLIKSQTTSIENGNGEDDQTKQAIYNILKEGSIEKEEAIKRKASLIHERFCGVKAPQISVTDSNMKTELEQAFGRNTQLQQQLDKSRRELQENQDQMVELRMDREGAESRLRQQEDQLAQLQEELRRTLENSPQSDSMQLDLLTVQAELSESQLLRQKLEDTLRQRERELTALKGALKDEVASHDKEMEALREQFSQDMDALRHSMETVSQSQLEIEEERQKVNASILAMEEELEGYKEQSEQWKKQFSSANQERGQYSAFQKLLKAQQGKRELEEKLLAVVKQTDETDSNSVMKELQQCRDSLKKAQSELEKQKAETLKKQEELKSATRASEKRETELKAEIDRLINQLKKEKEELSKAIEKTQQPLVSDQTKDPESNLELQEANARLRERIARMTRLHSSVPDSSSSDALEEENRSLKTQLEESRRAASRLGVEKEELNRRLEEREREREALRRGKSDLEEQKRLLDRSLDKINKEMESMMGDSRQSVQVLQSQLEEFRDRSRRELQDAQRLSKDRLVELQRAQALLKTTQEEVSRVKKELLSCTEERDSTQLDKELLSSRLKNMETELQTDRSSQTDRSREIRLLEDKVKTLEIELDEEKSGAELLNERITRCREQVDQLRSELMQERSARHDLEMDKSALERQIKELKSRIADMGTQSRPSAGVTMLENKVQELEDRLRSEEREKNTIQAAQRRLDRKLKDVTATLDQERNQHAEQRDQLSLRVKALKRQLDESEGEVERLEGVRRKVLRELEEQRELQAALQAKVNAMDNELKRKIQQSRRSTLGSTLSSDEEDNYSDTKSITSILTDSPLQTTSC

Target ORF information:

RefSeq Version	XM_005159385.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin b (cgnb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159385.4

ORF Insert Sequence:

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AACGAGGGCA GTACAGCGCG TTTCAAAAGC TTCTGAAGGC TCAGCAGGGC 
AAGAGGGAAC TAGAAGAGAA GCTGCTTGCA GTTGTAAAAC AGACCGATGA AACTGATTCA 
AACTCAGTGA TGAAAGAGTT GCAGCAGTGC CGAGATAGTC TGAAAAAAGC TCAATCAGAA 
CTGGAAAAAC AAAAAGCAGA GACGCTGAAA AAACAGGAGG AGCTTAAATC AGCTACGAGA 
GCCAGTGAGA AGCGGGAAAC TGAGCTGAAG GCAGAAATCG ACAGACTAAT AAATCAGTTA 
AAGAAAGAAA AAGAGGAGCT TTCAAAAGCA ATAGAGAAAA CACAACAGCC TTTAGTATCA 
GATCAAACCA AGGATCCTGA AAGCAATCTA GAACTTCAAG AGGCCAATGC TCGCTTGAGG 
GAGAGAATAG CACGCATGAC GCGGCTGCAC TCGAGTGTTC CTGACAGCAG CTCGTCTGAT 
GCTCTGGAGG AGGAGAACCG CAGCCTGAAG ACTCAGCTGG AGGAGTCGCG GCGCGCAGCC 
TCTCGACTGG GTGTGGAGAA AGAGGAGCTG AACCGACGGC TGGAGGAGCG TGAGAGAGAG 
AGGGAAGCTC TGAGACGCGG CAAGAGCGAC CTGGAGGAGC AGAAGAGACT GCTGGACCGA 
TCCCTGGACA AGATCAACAA GGAGATGGAG TCTATGATGG GAGACTCTCG TCAGTCGGTG 
CAGGTTCTTC AGTCTCAGCT GGAAGAATTC AGGGATCGTT CTCGCAGAGA GCTGCAGGAC 
GCTCAAAGAC TCAGTAAAGA CAGACTGGTT GAGCTGCAGA GAGCGCAGGC CCTCCTCAAA 
ACCACCCAGG AGGAGGTGTC TCGTGTGAAG AAGGAGCTGC TGTCATGCAC AGAGGAGAGA 
GACAGCACCC AGCTGGATAA AGAGCTCCTG AGCAGCCGAC TTAAAAACAT GGAGACAGAG 
CTGCAGACAG ACAGAAGCTC ACAGACGGAC CGCAGCAGAG AGATTCGCCT CTTAGAGGAC 
AAAGTGAAGA CATTAGAGAT CGAGCTGGAC GAGGAGAAGA GCGGAGCGGA GCTACTGAAC 
GAACGCATCA CTCGCTGTCG AGAACAGGTG GATCAGCTTC GATCTGAACT GATGCAGGAG 
CGTTCAGCCA GACATGATCT TGAGATGGAT AAAAGTGCGC TGGAGAGACA GATTAAGGAG 
TTGAAGTCTC GTATTGCTGA TATGGGCACT CAGTCTCGTC CCTCTGCTGG AGTCACAATG 
CTGGAAAACA AGGTTCAGGA GCTGGAGGAC CGTCTGCGCA GTGAAGAAAG AGAGAAAAAC 
ACCATTCAGG CAGCGCAGAG GAGATTGGAC AGGAAACTAA AGGATGTGAC GGCCACCCTT 
GACCAGGAGC GAAATCAACA CGCTGAACAG AGAGACCAGC TGTCTCTGCG AGTGAAGGCT 
CTGAAGCGTC AGCTGGATGA GAGCGAGGGT GAGGTGGAGC GTCTGGAGGG TGTCAGACGG 
AAGGTTCTGC GGGAGCTCGA GGAGCAGCGG GAGCTTCAGG CGGCGCTGCA GGCCAAAGTC 
AACGCAATGG ACAACGAGCT CAAGAGGAAG ATTCAGCAGT CTCGACGGTC GACTCTGGGA 
AGCACATTAA GCTCTGACGA GGAGGACAAT TATTCAGACA CCAAGAGCAT CACATCTATT 
CTCACCGATT CACCACTTCA GACCACCAGC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnb ( XM_005159386.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159443.1 Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.

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CloneID	ODa23844
Clone ID Related Accession (Same CDS sequence)	XM_005159386.3 , XM_005159386.4
Accession Version	XM_005159386.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cingulin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTCAAGAGG 
AAGATTCAGC AGTCTCGACG GTCGACTCTG GGAAGCACAT TAAGCTCTGA CGAGGAGGAC 
AATTATTCAG ACACCAAGAG CATCACATCT ATTCTCACCG ATTCACCACT TCAGACCACC 
AGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159443.1
CDS	208..3696
Translation	MSSLSADRKPPLDYGVQIRFIKDLDDAGGGFPDKSRLANGVGGVSNGTSSPSKYGVAVRVQGISGQPYVVLQDGEKGDSYGVQLKTQPQIQSAPPVVSQTSPYNTLLLGQREGARTPQGSYNPTDQPSSPDEDFGSPLRRPPGDGQAGTQGEAEPRTTERFTPSALAPKLDKKDNDAFNEAGLRKVKQNGIGSSLNGTGLNGSFPPPSSAQDEDQAPAIDTKSLAPINKLISRFGGGGGGSGDILNSEAQTRPRLDNRVRSQSADALNKPSEEASSTSPTINPYAPNTSATVPKLNSTKPSSTGSLGRDATSVAKVAAIPNFKPLAFNQSPKLFAPKEAPPAVPKKPVTPDLIKSQTTSIENGNGEDDQTKQAIYNILKEGSIEKEEAIKRKASLIHERFCGVKAPQISVTDSNMKTELEQAFGRNTQLQQQLDKSRRELQENQDQMVELRMDREGAESRLRQQEDQLAQLQEELRRTLENSPQSDSMQLDLLTVQAELSESQLLRQKLEDTLRQRERELTALKGALKDEVASHDKEMEALREQFSQDMDALRHSMETVSQSQLEIEEERQKVNASILAMEEELEGYKEQSEQWKKQFSSANQELLKAQQGKRELEEKLLAVVKQTDETDSNSVMKELQQCRDSLKKAQSELEKQKAETLKKQEELKSATRASEKRETELKAEIDRLINQLKKEKEELSKAIEKTQQPLVSDQTKDPESNLELQEANARLRERIARMTRLHSSVPDSSSSDALEEENRSLKTQLEESRRAASRLGVEKEELNRRLEEREREREALRRGKSDLEEQKRLLDRSLDKINKEMESMMGDSRQSVQVLQSQLEEFRDRSRRELQDAQRLSKDRLVELQRAQALLKTTQEEVSRVKKELLSCTEERDSTQLDKELLSSRLKNMETELQTDRSSQTDRSREIRLLEDKVKTLEIELDEEKSGAELLNERITRCREQVDQLRSELMQERSARHDLEMDKSALERQIKELKSRIADMGTQSRPSAGVTMLENKVQELEDRLRSEEREKNTIQAAQRRLDRKLKDVTATLDQERNQHAEQRDQLSLRVKALKRQLDESEGEVERLEGVRRKVLRELEEQRELQAALQAKVNAMDNELKRKIQQSRRSTLGSTLSSDEEDNYSDTKSITSILTDSPLQTTSC

Target ORF information:

RefSeq Version	XM_005159386.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159386.3

ORF Insert Sequence:

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTCAAGAGG 
AAGATTCAGC AGTCTCGACG GTCGACTCTG GGAAGCACAT TAAGCTCTGA CGAGGAGGAC 
AATTATTCAG ACACCAAGAG CATCACATCT ATTCTCACCG ATTCACCACT TCAGACCACC 
AGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnb ( XM_005159386.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159443.1 Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23844
Clone ID Related Accession (Same CDS sequence)	XM_005159386.3 , XM_005159386.4
Accession Version	XM_005159386.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159386.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTCAAGAGG 
AAGATTCAGC AGTCTCGACG GTCGACTCTG GGAAGCACAT TAAGCTCTGA CGAGGAGGAC 
AATTATTCAG ACACCAAGAG CATCACATCT ATTCTCACCG ATTCACCACT TCAGACCACC 
AGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159443.1
CDS	308..3796
Translation	MSSLSADRKPPLDYGVQIRFIKDLDDAGGGFPDKSRLANGVGGVSNGTSSPSKYGVAVRVQGISGQPYVVLQDGEKGDSYGVQLKTQPQIQSAPPVVSQTSPYNTLLLGQREGARTPQGSYNPTDQPSSPDEDFGSPLRRPPGDGQAGTQGEAEPRTTERFTPSALAPKLDKKDNDAFNEAGLRKVKQNGIGSSLNGTGLNGSFPPPSSAQDEDQAPAIDTKSLAPINKLISRFGGGGGGSGDILNSEAQTRPRLDNRVRSQSADALNKPSEEASSTSPTINPYAPNTSATVPKLNSTKPSSTGSLGRDATSVAKVAAIPNFKPLAFNQSPKLFAPKEAPPAVPKKPVTPDLIKSQTTSIENGNGEDDQTKQAIYNILKEGSIEKEEAIKRKASLIHERFCGVKAPQISVTDSNMKTELEQAFGRNTQLQQQLDKSRRELQENQDQMVELRMDREGAESRLRQQEDQLAQLQEELRRTLENSPQSDSMQLDLLTVQAELSESQLLRQKLEDTLRQRERELTALKGALKDEVASHDKEMEALREQFSQDMDALRHSMETVSQSQLEIEEERQKVNASILAMEEELEGYKEQSEQWKKQFSSANQELLKAQQGKRELEEKLLAVVKQTDETDSNSVMKELQQCRDSLKKAQSELEKQKAETLKKQEELKSATRASEKRETELKAEIDRLINQLKKEKEELSKAIEKTQQPLVSDQTKDPESNLELQEANARLRERIARMTRLHSSVPDSSSSDALEEENRSLKTQLEESRRAASRLGVEKEELNRRLEEREREREALRRGKSDLEEQKRLLDRSLDKINKEMESMMGDSRQSVQVLQSQLEEFRDRSRRELQDAQRLSKDRLVELQRAQALLKTTQEEVSRVKKELLSCTEERDSTQLDKELLSSRLKNMETELQTDRSSQTDRSREIRLLEDKVKTLEIELDEEKSGAELLNERITRCREQVDQLRSELMQERSARHDLEMDKSALERQIKELKSRIADMGTQSRPSAGVTMLENKVQELEDRLRSEEREKNTIQAAQRRLDRKLKDVTATLDQERNQHAEQRDQLSLRVKALKRQLDESEGEVERLEGVRRKVLRELEEQRELQAALQAKVNAMDNELKRKIQQSRRSTLGSTLSSDEEDNYSDTKSITSILTDSPLQTTSC

Target ORF information:

RefSeq Version	XM_005159386.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin b (cgnb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159386.4

ORF Insert Sequence:

ATGAGTTCCC TCTCTGCGGA CAGGAAGCCC CCTCTAGACT ATGGTGTCCA GATTCGCTTC 
ATCAAAGATT TGGATGATGC TGGCGGAGGG TTTCCAGACA AGAGTCGACT GGCCAATGGA 
GTTGGTGGCG TCAGCAATGG GACTTCTTCG CCATCTAAGT ATGGAGTAGC TGTGAGAGTT 
CAAGGCATTT CAGGACAGCC CTATGTGGTG CTCCAGGATG GAGAAAAGGG GGACTCTTAT 
GGAGTACAAC TGAAGACTCA ACCACAAATT CAGAGCGCAC CCCCAGTTGT CTCTCAGACA 
TCCCCATATA ACACCCTGCT TCTGGGACAA AGGGAAGGGG CTAGGACTCC CCAAGGTTCT 
TATAACCCTA CTGACCAACC TAGTTCTCCA GATGAGGACT TTGGGAGCCC CTTAAGGAGG 
CCACCTGGTG ATGGACAGGC AGGGACTCAA GGTGAAGCCG AGCCCAGGAC TACAGAACGC 
TTCACCCCAT CTGCTCTTGC TCCCAAGTTA GACAAAAAGG ACAATGATGC TTTCAACGAA 
GCAGGCCTTA GGAAGGTCAA ACAGAATGGG ATTGGGAGTA GTCTGAATGG GACAGGGCTA 
AATGGATCTT TCCCTCCTCC CTCTTCTGCA CAGGATGAGG ATCAGGCCCC AGCTATAGAC 
ACCAAATCCT TGGCTCCGAT TAATAAACTC ATTAGCCGCT TTGGTGGTGG CGGTGGTGGT 
TCCGGTGATA TTCTTAACTC GGAAGCCCAA ACTCGGCCAC GCCTTGATAA CAGAGTTCGC 
TCTCAGAGTG CAGATGCTCT CAACAAACCT TCTGAAGAGG CCTCATCAAC CTCGCCAACA 
ATAAACCCCT ATGCCCCCAA TACTTCAGCT ACAGTACCCA AACTCAACTC AACAAAACCA 
TCATCAACTG GTAGTCTGGG ACGAGATGCC ACCTCTGTAG CTAAAGTGGC TGCTATCCCC 
AATTTCAAAC CTCTTGCTTT CAACCAATCA CCAAAGCTGT TTGCACCCAA GGAGGCACCA 
CCTGCTGTAC CAAAGAAACC AGTGACCCCT GACCTCATAA AGAGCCAGAC TACAAGTATA 
GAGAATGGAA ATGGAGAGGA TGACCAGACC AAACAAGCCA TTTACAACAT ACTTAAAGAA 
GGGTCTATTG AGAAGGAGGA AGCCATCAAG CGAAAAGCAA GCCTCATTCA TGAAAGGTTC 
TGTGGGGTGA AGGCTCCTCA GATCTCAGTG ACTGACAGCA ATATGAAGAC TGAACTGGAG 
CAGGCCTTCG GCAGAAACAC ACAGTTACAG CAACAGCTGG ACAAGAGCAG GAGAGAACTG 
CAGGAAAACC AGGACCAAAT GGTGGAGCTG CGCATGGATC GGGAGGGAGC GGAGTCTCGC 
TTGCGGCAGC AGGAAGATCA GCTCGCTCAG CTTCAAGAGG AGCTCAGGAG AACACTGGAG 
AACTCGCCGC AGTCTGACAG CATGCAGCTG GACCTGTTGA CGGTGCAGGC CGAACTGTCG 
GAGTCGCAGT TGCTGAGGCA GAAGCTGGAG GACACTTTGA GACAGAGAGA GCGAGAACTC 
ACCGCGCTAA AAGGAGCCCT GAAGGACGAG GTGGCGTCTC ACGATAAAGA GATGGAGGCC 
CTGAGAGAGC AGTTCAGTCA AGACATGGAT GCCCTGCGTC ACAGCATGGA GACCGTCTCA 
CAGTCTCAGC TGGAAATTGA GGAGGAGCGT CAGAAGGTCA ACGCCTCCAT CTTGGCTATG 
GAGGAAGAGC TTGAGGGCTA CAAAGAGCAG AGCGAGCAAT GGAAGAAGCA GTTTAGTTCC 
GCCAACCAAG AGCTTCTGAA GGCTCAGCAG GGCAAGAGGG AACTAGAAGA GAAGCTGCTT 
GCAGTTGTAA AACAGACCGA TGAAACTGAT TCAAACTCAG TGATGAAAGA GTTGCAGCAG 
TGCCGAGATA GTCTGAAAAA AGCTCAATCA GAACTGGAAA AACAAAAAGC AGAGACGCTG 
AAAAAACAGG AGGAGCTTAA ATCAGCTACG AGAGCCAGTG AGAAGCGGGA AACTGAGCTG 
AAGGCAGAAA TCGACAGACT AATAAATCAG TTAAAGAAAG AAAAAGAGGA GCTTTCAAAA 
GCAATAGAGA AAACACAACA GCCTTTAGTA TCAGATCAAA CCAAGGATCC TGAAAGCAAT 
CTAGAACTTC AAGAGGCCAA TGCTCGCTTG AGGGAGAGAA TAGCACGCAT GACGCGGCTG 
CACTCGAGTG TTCCTGACAG CAGCTCGTCT GATGCTCTGG AGGAGGAGAA CCGCAGCCTG 
AAGACTCAGC TGGAGGAGTC GCGGCGCGCA GCCTCTCGAC TGGGTGTGGA GAAAGAGGAG 
CTGAACCGAC GGCTGGAGGA GCGTGAGAGA GAGAGGGAAG CTCTGAGACG CGGCAAGAGC 
GACCTGGAGG AGCAGAAGAG ACTGCTGGAC CGATCCCTGG ACAAGATCAA CAAGGAGATG 
GAGTCTATGA TGGGAGACTC TCGTCAGTCG GTGCAGGTTC TTCAGTCTCA GCTGGAAGAA 
TTCAGGGATC GTTCTCGCAG AGAGCTGCAG GACGCTCAAA GACTCAGTAA AGACAGACTG 
GTTGAGCTGC AGAGAGCGCA GGCCCTCCTC AAAACCACCC AGGAGGAGGT GTCTCGTGTG 
AAGAAGGAGC TGCTGTCATG CACAGAGGAG AGAGACAGCA CCCAGCTGGA TAAAGAGCTC 
CTGAGCAGCC GACTTAAAAA CATGGAGACA GAGCTGCAGA CAGACAGAAG CTCACAGACG 
GACCGCAGCA GAGAGATTCG CCTCTTAGAG GACAAAGTGA AGACATTAGA GATCGAGCTG 
GACGAGGAGA AGAGCGGAGC GGAGCTACTG AACGAACGCA TCACTCGCTG TCGAGAACAG 
GTGGATCAGC TTCGATCTGA ACTGATGCAG GAGCGTTCAG CCAGACATGA TCTTGAGATG 
GATAAAAGTG CGCTGGAGAG ACAGATTAAG GAGTTGAAGT CTCGTATTGC TGATATGGGC 
ACTCAGTCTC GTCCCTCTGC TGGAGTCACA ATGCTGGAAA ACAAGGTTCA GGAGCTGGAG 
GACCGTCTGC GCAGTGAAGA AAGAGAGAAA AACACCATTC AGGCAGCGCA GAGGAGATTG 
GACAGGAAAC TAAAGGATGT GACGGCCACC CTTGACCAGG AGCGAAATCA ACACGCTGAA 
CAGAGAGACC AGCTGTCTCT GCGAGTGAAG GCTCTGAAGC GTCAGCTGGA TGAGAGCGAG 
GGTGAGGTGG AGCGTCTGGA GGGTGTCAGA CGGAAGGTTC TGCGGGAGCT CGAGGAGCAG 
CGGGAGCTTC AGGCGGCGCT GCAGGCCAAA GTCAACGCAA TGGACAACGA GCTCAAGAGG 
AAGATTCAGC AGTCTCGACG GTCGACTCTG GGAAGCACAT TAAGCTCTGA CGAGGAGGAC 
AATTATTCAG ACACCAAGAG CATCACATCT ATTCTCACCG ATTCACCACT TCAGACCACC 
AGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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cgnb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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