LOC561423 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC561423
Entrez Gene ID	561423
Full Name	cingulin-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169753.3	XP_005169810.1	cingulin-like isoform X2
XM_009292291.3	XP_009290566.1	cingulin-like isoform X1
XM_009292291.2	XP_009290566.1	cingulin-like isoform X1
XM_021472986.1	XP_021328661.1	cingulin-like isoform X2
XM_005169753.4	XP_005169810.1	cingulin-like isoform X2
XM_021472985.1	XP_021328660.1	cingulin-like isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cgn	NP_001032800.2
Gallus gallus (chicken)	CGN	XP_004948438.1
Danio rerio (zebrafish)	LOC561423	XP_689920.3
Pan troglodytes (chimpanzee)	CGN	XP_513800.2
Danio rerio (zebrafish)	cgnb	NP_001038227.1
Bos taurus (cattle)	CGN	NP_001179715.1
	LOC710830	XP_001108356.1
Xenopus tropicalis (tropical clawed frog)	cgn	NP_001120598.1
Homo sapiens (human)	CGN	NP_065821.1
Canis lupus familiaris (dog)	CGN	NP_001096687.1
Rattus norvegicus (Norway rat)	Cgn	XP_006232990.1

Gene Ontology

The following LOC561423 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC561423 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21389	XM_005169753.4 Latest version!	Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa21389	XM_021472986.1 Latest version!	Danio rerio cingulin-like (LOC561423), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43504	XM_009292291.3 Latest version!	Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43504	XM_021472985.1 Latest version!	Danio rerio cingulin-like (LOC561423), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa43504	XM_009292291.2	Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa21389	XM_005169753.3	Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC561423 ( XM_005169753.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169810.1 Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.

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CloneID	ODa21389
Clone ID Related Accession (Same CDS sequence)	XM_005169753.3 , XM_021472986.1 , XM_005169753.4
Accession Version	XM_005169753.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cingulin-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169810.1
CDS	163..3711
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_005169753.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169753.3

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC561423 ( XM_021472986.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328661.1 Danio rerio cingulin-like (LOC561423), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa21389
Clone ID Related Accession (Same CDS sequence)	XM_005169753.3 , XM_021472986.1 , XM_005169753.4
Accession Version	XM_021472986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328661.1
CDS	20..3568
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_021472986.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472986.1

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC561423 ( XM_005169753.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169810.1 Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.

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CloneID	ODa21389
Clone ID Related Accession (Same CDS sequence)	XM_005169753.3 , XM_021472986.1 , XM_005169753.4
Accession Version	XM_005169753.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169753.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169810.1
CDS	163..3711
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_005169753.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169753.4

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGAATTACTC CAGTCACGTC TGGAGAAAGA AGAGTTTGAG 
GAGGAGCTGA ATGATCTGCA GGAGAAAGTC AACACAATGA AACAGCAGAT GCCAGATCCT 
AAACAGACAC AGGCGGTCAG CCAGGAACTT GAACGATGTC GTGCTGACCT GCAGAAGACC 
CAGGCAGATA TGGATAAATT AAGGGTTGAT CTTGACAAGA AGACAATGGA AATTGTGTTA 
TTAAAGAAGA GCAAACAAGA GCTTGAAGCA GAGCAGAAAT ATGAGATTGA CCGATTGAAG 
GGTCAGTCAC GAAGAGATAA GGAAGAGCTC ACAAAGGCTC ATGAGAGGGC AAAGCAGCTT 
GCTGAACCCT CGTTGGTGGA GGCCCTGCGG AAGGAGTTGA GTGACATGCA AGAGGAGGCA 
GGTCGACTGC GAGGTCAGCT GATCTCCACT GAAGAAATGC TGCAGGCAGA GAAAGACAAA 
CTCAGCTCTG CTCAAATTCA AGTCAACAAT CTGAAGCTGG AGCATACGGA ACTGGAGGAA 
ACCAACATGC GTATGAAGGA GAGAATCACA CGCCTAGAGT CTCAGCTGCA GGAACGCATG 
TCTCAGAGTT TTGAGGCTGA GCAGGAGCAG CAGGAGGAGG CCAGGAAACT GAGGCAGCAG 
CTGGAGGAGG CCAGGCGAGA GAGCTCCAGG CTGAGCCTGG AGAGAGATGA GCTGGCCCGT 
AATCTGGAAG AGAAGGAGAA GGACAGAGAC ACTGTCCGTA AGGAGAACAC GCAGCTCGAT 
GACCAGAGGA GACAGCAGGA GAGAGCGCTA GACAAACTTA ATAAAGAGAT GGAGCGCTTG 
TCTGCTACAC ATCGTGAGGA AATGCGGCTC CTGCAAGCTC AGCTAGACGA GCAGAGAGAC 
AAGTGGAGGA AAGAGCAGCA GGACTCACAG AAAATCACCA AAGAGAAGCT CAGTGAGCTG 
GATAAAGCCC AGAGCACCAT CCACTCCCTG CAGGAGGAGC TGGGCCGTGT AAAGAAAGAG 
CTGTTCTCCA GTTATGAGGA AAGAGACAAC GCTTTATTAG ACAAAGAGAT GCTCACCAAC 
CGCCTCAAAC ATCTGGAGAG TGAGATGGAG ACTCAGCGCA ACACACAAAA CGACCGCTCA 
AGAGAGATCC GCAGTTTAGA GGACAAAATC AAGCATTTGG AGCTCGAGTT GGATGAGGAG 
AAGAACAGTG CAGAGATGCT GACAGAAAGA ATCACCAGGA GCCGAGATCA GATTGAACAG 
CTGCGTGCAG AACTCATGCA AGAGAGATCC TCCAAACAAG ACCTGGAGCT AGACAAAAAC 
GCTCTGGAGA GACAGATAAA GGAGTATAAG ACTCGTGTAG CTGAGATGGA AGGCCAGTCA 
CGTTCCTCCA CTGGTGTCTC ACAGCTGGAG AGCAAAATCC AGGAACTCGA GGAACGACTG 
CGCACAGAAG AGAGAGAGAA GAACTCTGTG GTTTCCTCTC AGCGCCGGAT AGAGAGGAAG 
CTAAAGGAAC TGAACATCAC TCTGGATGAG GAGAGACAGC AGCACACAGA GCAGAGAGAC 
CAGCTGACTC TGCGTGTTAA GGCTCTGAAA CGGCAGGTGG ATGAAGGAGA GGCAGAGGCG 
GAGCGTCTGG AGGGCTTGAG GAGGAAGGCC ATCAGAGAGA TGGAGGAGCA GCAGGAGCAG 
AAGGAGGCGC TGCAGTCCAG AGTCACAGCG CTAGAGAATG AACTCAAGAG GAAGATTCAG 
CAGGCCCGAC AGTCAGTGCT GGAATCTTCA GTGCTCAGCT CTGACGATGA CGATGATGAT 
GGATTGTACG ATCACTCCAG CATAACCTCC ATCCTCACCG AGAGCAATTT ACAGACCAGT 
TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC561423 ( XM_009292291.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290566.1 Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.

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CloneID	ODa43504
Clone ID Related Accession (Same CDS sequence)	XM_009292291.3 , XM_009292291.2 , XM_021472985.1
Accession Version	XM_009292291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290566.1
CDS	144..3719
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQDGQLVQPKAELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_009292291.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292291.3

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC561423 ( XM_009292291.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290566.1 Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43504
Clone ID Related Accession (Same CDS sequence)	XM_009292291.3 , XM_009292291.2 , XM_021472985.1
Accession Version	XM_009292291.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cingulin-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290566.1
CDS	132..3707
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQDGQLVQPKAELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_009292291.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292291.2

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC561423 ( XM_021472985.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328660.1 Danio rerio cingulin-like (LOC561423), transcript variant X3, mRNA.

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CloneID	ODa43504
Clone ID Related Accession (Same CDS sequence)	XM_009292291.3 , XM_009292291.2 , XM_021472985.1
Accession Version	XM_021472985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cingulin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328660.1
CDS	20..3595
Translation	MSAPVSGRKTPVDYGVQIRFINDLQDTGGGVSGQPRKEGPKKQPSRYGVAVRVQGIGGQPYVVLKEGEKGDSYGVQLRTQPPAYSSLPRRREDGVIQGPYFSSGDNIPIRRAQSHGSLLDRENSPEDFTDQLRRPLGDGRSGSYGNLDGGIGMGSERERPRERMERNQWGGSYQTGLNGTLGRGRGGGSHHTSSESIEIALHNQHTQIQSAPSSTRQTPVQRLVNRFDGSSSQVDNSATTTRGRYPVPNADDRSTSTSPLPAPSLIPKTYSSPSSSSPASAYSSLGRGSGSIAKVTAVTPSVSSDGHVTPDLLMDQGQSSGSEFSPEDQIQQIIYDALRQGSTEGDVAIKRKARVICNKIQGLKASRSDDSLKHELEKCLDENIQLQEQLGRKNTELHQTHSDLTQLRMDRENAESHVRELEDQLAGLQEELRRETENKAQADTMHMELMALRAELDEAAVLRQKQEDIQRQRERELTALKGALKDEVSTHDKEIEALREQYSQDMEQLRTSMAQVSQSQATIEAERHRVNSTLRSLQQQLEESRDEGNHWREQFQSSREELRNTKQDGQLVQPKAELLQSRLEKEEFEEELNDLQEKVNTMKQQMPDPKQTQAVSQELERCRADLQKTQADMDKLRVDLDKKTMEIVLLKKSKQELEAEQKYEIDRLKGQSRRDKEELTKAHERAKQLAEPSLVEALRKELSDMQEEAGRLRGQLISTEEMLQAEKDKLSSAQIQVNNLKLEHTELEETNMRMKERITRLESQLQERMSQSFEAEQEQQEEARKLRQQLEEARRESSRLSLERDELARNLEEKEKDRDTVRKENTQLDDQRRQQERALDKLNKEMERLSATHREEMRLLQAQLDEQRDKWRKEQQDSQKITKEKLSELDKAQSTIHSLQEELGRVKKELFSSYEERDNALLDKEMLTNRLKHLESEMETQRNTQNDRSREIRSLEDKIKHLELELDEEKNSAEMLTERITRSRDQIEQLRAELMQERSSKQDLELDKNALERQIKEYKTRVAEMEGQSRSSTGVSQLESKIQELEERLRTEEREKNSVVSSQRRIERKLKELNITLDEERQQHTEQRDQLTLRVKALKRQVDEGEAEAERLEGLRRKAIREMEEQQEQKEALQSRVTALENELKRKIQQARQSVLESSVLSSDDDDDDGLYDHSSITSILTESNLQTSSC

Target ORF information:

RefSeq Version	XM_021472985.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cingulin-like (LOC561423), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472985.1

ORF Insert Sequence:

ATGTCTGCTC CTGTCTCCGG TAGGAAAACC CCTGTGGACT ATGGTGTACA GATTCGATTT 
ATAAATGACC TGCAAGACAC CGGAGGGGGA GTGAGTGGGC AGCCCCGGAA AGAGGGACCT 
AAAAAGCAGC CGTCTCGCTA TGGAGTGGCA GTGAGAGTGC AGGGCATTGG TGGACAGCCC 
TACGTCGTAC TGAAAGAGGG AGAGAAAGGA GATTCATATG GAGTGCAGTT GAGAACCCAA 
CCACCTGCCT ACAGCAGCCT GCCTAGGAGG AGAGAGGATG GTGTAATCCA GGGGCCATAT 
TTCTCATCTG GAGACAACAT CCCTATCCGT AGGGCTCAGT CTCATGGCTC ATTGTTGGAC 
AGAGAAAACA GTCCTGAAGA CTTCACAGAT CAGCTCCGAC GTCCACTTGG AGATGGACGT 
TCTGGAAGCT ATGGCAACCT GGATGGAGGT ATTGGTATGG GATCTGAAAG GGAGCGACCC 
AGAGAAAGGA TGGAGAGAAA TCAGTGGGGA GGTTCCTACC AGACAGGGCT GAATGGCACT 
TTGGGGAGAG GGAGAGGTGG AGGCAGTCAC CACACCTCAT CCGAATCCAT CGAAATAGCA 
CTGCACAACC AGCACACTCA GATCCAATCA GCCCCCAGCT CCACCCGTCA GACCCCTGTC 
CAAAGACTTG TCAACAGGTT CGACGGGAGC TCTTCACAGG TGGATAACTC TGCAACCACC 
ACCAGGGGGC GCTATCCTGT TCCAAATGCA GACGACAGAT CCACTTCAAC TTCACCTTTA 
CCTGCTCCAT CTCTCATTCC TAAAACATAC TCTTCACCAT CATCTTCGTC ACCTGCTTCT 
GCTTACAGCA GCCTGGGTCG TGGATCCGGT TCTATTGCAA AAGTTACAGC AGTCACTCCC 
TCTGTTTCAT CTGATGGACA TGTCACTCCA GACCTCTTGA TGGACCAGGG TCAAAGTTCA 
GGGTCAGAGT TCTCACCTGA AGACCAGATC CAGCAGATAA TCTATGATGC TCTCCGTCAG 
GGGAGTACAG AGGGAGATGT TGCCATTAAG CGCAAAGCTC GAGTCATCTG TAATAAAATT 
CAAGGACTTA AGGCAAGCAG ATCTGATGAC TCCTTAAAAC ATGAGCTTGA GAAATGTCTG 
GATGAAAATA TCCAGCTGCA GGAACAGCTG GGGAGGAAGA ACACAGAACT GCACCAAACA 
CATTCAGATC TTACTCAGTT ACGTATGGAC AGAGAGAACG CAGAGTCTCA TGTTAGAGAG 
TTGGAGGATC AGCTGGCAGG ACTGCAGGAG GAGCTGAGGA GAGAAACTGA AAACAAAGCT 
CAAGCTGACA CAATGCACAT GGAATTGATG GCATTGAGAG CTGAATTGGA TGAAGCTGCA 
GTGCTGAGAC AAAAGCAGGA GGACATTCAG AGGCAAAGAG AGAGGGAGCT GACGGCCCTG 
AAGGGGGCAT TAAAAGATGA GGTCTCCACC CATGACAAGG AGATAGAGGC TCTCAGAGAG 
CAGTACAGCC AAGACATGGA ACAACTGCGA ACAAGCATGG CTCAGGTGTC ACAGTCTCAG 
GCAACTATTG AAGCCGAGCG CCACCGTGTA AACTCTACGT TAAGGTCTCT TCAGCAGCAA 
CTGGAGGAGA GCAGAGATGA AGGAAACCAC TGGAGAGAGC AGTTCCAGTC CAGCAGAGAA 
GAGCTGCGTA ACACCAAACA AGATGGACAG CTGGTTCAAC CCAAAGCAGA ATTACTCCAG 
TCACGTCTGG AGAAAGAAGA GTTTGAGGAG GAGCTGAATG ATCTGCAGGA GAAAGTCAAC 
ACAATGAAAC AGCAGATGCC AGATCCTAAA CAGACACAGG CGGTCAGCCA GGAACTTGAA 
CGATGTCGTG CTGACCTGCA GAAGACCCAG GCAGATATGG ATAAATTAAG GGTTGATCTT 
GACAAGAAGA CAATGGAAAT TGTGTTATTA AAGAAGAGCA AACAAGAGCT TGAAGCAGAG 
CAGAAATATG AGATTGACCG ATTGAAGGGT CAGTCACGAA GAGATAAGGA AGAGCTCACA 
AAGGCTCATG AGAGGGCAAA GCAGCTTGCT GAACCCTCGT TGGTGGAGGC CCTGCGGAAG 
GAGTTGAGTG ACATGCAAGA GGAGGCAGGT CGACTGCGAG GTCAGCTGAT CTCCACTGAA 
GAAATGCTGC AGGCAGAGAA AGACAAACTC AGCTCTGCTC AAATTCAAGT CAACAATCTG 
AAGCTGGAGC ATACGGAACT GGAGGAAACC AACATGCGTA TGAAGGAGAG AATCACACGC 
CTAGAGTCTC AGCTGCAGGA ACGCATGTCT CAGAGTTTTG AGGCTGAGCA GGAGCAGCAG 
GAGGAGGCCA GGAAACTGAG GCAGCAGCTG GAGGAGGCCA GGCGAGAGAG CTCCAGGCTG 
AGCCTGGAGA GAGATGAGCT GGCCCGTAAT CTGGAAGAGA AGGAGAAGGA CAGAGACACT 
GTCCGTAAGG AGAACACGCA GCTCGATGAC CAGAGGAGAC AGCAGGAGAG AGCGCTAGAC 
AAACTTAATA AAGAGATGGA GCGCTTGTCT GCTACACATC GTGAGGAAAT GCGGCTCCTG 
CAAGCTCAGC TAGACGAGCA GAGAGACAAG TGGAGGAAAG AGCAGCAGGA CTCACAGAAA 
ATCACCAAAG AGAAGCTCAG TGAGCTGGAT AAAGCCCAGA GCACCATCCA CTCCCTGCAG 
GAGGAGCTGG GCCGTGTAAA GAAAGAGCTG TTCTCCAGTT ATGAGGAAAG AGACAACGCT 
TTATTAGACA AAGAGATGCT CACCAACCGC CTCAAACATC TGGAGAGTGA GATGGAGACT 
CAGCGCAACA CACAAAACGA CCGCTCAAGA GAGATCCGCA GTTTAGAGGA CAAAATCAAG 
CATTTGGAGC TCGAGTTGGA TGAGGAGAAG AACAGTGCAG AGATGCTGAC AGAAAGAATC 
ACCAGGAGCC GAGATCAGAT TGAACAGCTG CGTGCAGAAC TCATGCAAGA GAGATCCTCC 
AAACAAGACC TGGAGCTAGA CAAAAACGCT CTGGAGAGAC AGATAAAGGA GTATAAGACT 
CGTGTAGCTG AGATGGAAGG CCAGTCACGT TCCTCCACTG GTGTCTCACA GCTGGAGAGC 
AAAATCCAGG AACTCGAGGA ACGACTGCGC ACAGAAGAGA GAGAGAAGAA CTCTGTGGTT 
TCCTCTCAGC GCCGGATAGA GAGGAAGCTA AAGGAACTGA ACATCACTCT GGATGAGGAG 
AGACAGCAGC ACACAGAGCA GAGAGACCAG CTGACTCTGC GTGTTAAGGC TCTGAAACGG 
CAGGTGGATG AAGGAGAGGC AGAGGCGGAG CGTCTGGAGG GCTTGAGGAG GAAGGCCATC 
AGAGAGATGG AGGAGCAGCA GGAGCAGAAG GAGGCGCTGC AGTCCAGAGT CACAGCGCTA 
GAGAATGAAC TCAAGAGGAA GATTCAGCAG GCCCGACAGT CAGTGCTGGA ATCTTCAGTG 
CTCAGCTCTG ACGATGACGA TGATGATGGA TTGTACGATC ACTCCAGCAT AACCTCCATC 
CTCACCGAGA GCAATTTACA GACCAGTTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC561423 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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