CGN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CGN
Entrez Gene ID	57530
Full Name	cingulin
General protein information	Preferred Names cingulin Names cingulin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Disorder MIM:	609473

mRNA and Protein(s)

mRNA	Protein	Name
XM_005245365.4	XP_005245422.1	cingulin isoform X1
XM_005245365.5	XP_005245422.1	cingulin isoform X1
NM_020770.2	NP_065821.1	cingulin
NM_020770.3	NP_065821.1	cingulin

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cgn	NP_001032800.2
Gallus gallus (chicken)	CGN	XP_004948438.1
Danio rerio (zebrafish)	LOC561423	XP_689920.3
Pan troglodytes (chimpanzee)	CGN	XP_513800.2
Danio rerio (zebrafish)	cgnb	NP_001038227.1
Bos taurus (cattle)	CGN	NP_001179715.1
	LOC710830	XP_001108356.1
Xenopus tropicalis (tropical clawed frog)	cgn	NP_001120598.1
Homo sapiens (human)	CGN	NP_065821.1
Canis lupus familiaris (dog)	CGN	NP_001096687.1
Rattus norvegicus (Norway rat)	Cgn	XP_006232990.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Role of Cingulin in Agonist-induced Vascular Endothelial Permeability.
Tian Y, Gawlak G, Tian X, Shah AS, Sarich N, Citi S, Birukova AA
The Journal of biological chemistry291(45)23681-23692(2016 Nov)

Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo.
Schossleitner K, Rauscher S, Gr?ger M, Friedl HP, Finsterwalder R, Habertheuer A, Sibilia M, Brostjan C, F?dinger D, Citi S, Petzelbauer P
Arteriosclerosis, thrombosis, and vascular biology36(4)647-54(2016 Apr)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.
Guillemot L, Guerrera D, Spadaro D, Tapia R, Jond L, Citi S
Molecular biology of the cell25(13)1995-2005(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Down-regulation of Cingulin Augments Thrombin-induced Endothelial Barrier Disruption.
Title: Role of Cingulin in Agonist-induced Vascular Endothelial Permeability.

Cingulin modulates barrier function in endothelium.
Title: Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo.

MgcRacGAP colocalizes with CGN and CGNL1 at TJs and forms a complex and interacts directly in vitro with CGN and CGNL1.
Title: MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.

Co-imunoprecipitation and confocal microscopy experiments also revealed the tight junction protein cingulin as a new interacting partner of Ubn-1
Title: Identification of new interacting partners of the shuttling protein ubinuclein (Ubn-1).

Results indicate that cells underwent EMT exhibited overactive TGFbeta signaling and loss of expression of the CDH1, CGN, CLDN4, and KLK10 genes as a result of hypermethylation of their corresponding promoter regions.
Title: Smad signaling is required to maintain epigenetic silencing during breast cancer progression.

The following CGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31771	NM_020770.3 Latest version!	Homo sapiens cingulin (CGN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu31771	XM_005245365.5 Latest version!	Homo sapiens cingulin (CGN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
View Old Accession Versions >>
OHu31771	XM_005245365.4	Homo sapiens cingulin (CGN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu31771	NM_020770.2	Homo sapiens cingulin (CGN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CGN ( NM_020770.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065821.1 Homo sapiens cingulin (CGN), mRNA.

Price & Availability↑

CloneID	OHu31771
Clone ID Related Accession (Same CDS sequence)	XM_005245365.4 , XM_005245365.5 , NM_020770.2 , NM_020770.3
Accession Version	NM_020770.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cingulin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN359389.1, AF263462.1, AL365436.26, BC152445.1, BP331021.1 and BC146657.1. On May 7, 2008 this sequence version replaced NM_020770.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF263462.1, AB037740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065821.1
CDS	145..3756
Translation	MEQAPNMAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGLERQLEEKTEECSRLQELLERRKGEAQQSNKELQNMKRLLDQGEDLRHGLETQVMELQNKLKHVQGPEPAKEVLLKDLLETRELLEEVLEGKQRVEEQLRLRERELTALKGALKEEVASRDQEVEHVRQQYQRDTEQLRRSMQDATQDHAVLEAERQKMSALVRGLQRELEETSEETGHWQSMFQKNKEDLRATKQELLQLRMEKEEMEEELGEKIEVLQRELEQARASAGDTRQVEVLKKELLRTQEELKELQAERQSQEVAGRHRDRELEKQLAVLRVEADRGRELEEQNLQLQKTLQQLRQDCEEASKAKMVAEAEATVLGQRRAAVETTLRETQEENDEFRRRILGLEQQLKETRGLVDGGEAVEARLRDKLQRLEAEKQQLEEALNASQEEEGSLAAAKRALEARLEEAQRGLARLGQEQQTLNRALEEEGKQREVLRRGKAELEEQKRLLDRTVDRLNKELEKIGEDSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDEIQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEEKVSRLETELDEEKNTVELLTDRVNRGRDQVDQLRTELMQERSARQDLECDKISLERQNKDLKTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKTVLQSTNRKLERKVKELSIQIEDERQHVNDQKDQLSLRVKALKRQVDEAEEEIERLDGLRKKAQREVEEQHEVNEQLQARIKSLEKDSWRKASRSAAESALKNEGLSSDEEFDSVYDPSSIASLLTESNLQTSSC

Target ORF information:

RefSeq Version	NM_020770.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cingulin (CGN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020770.2

ORF Insert Sequence:

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGN ( NM_020770.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065821.1 Homo sapiens cingulin (CGN), mRNA.

Price & Availability↑

CloneID	OHu31771
Clone ID Related Accession (Same CDS sequence)	XM_005245365.4 , XM_005245365.5 , NM_020770.2 , NM_020770.3
Accession Version	NM_020770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	cingulin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN359389.1, AF263462.1, AL365436.26, BC152445.1, BP331021.1 and BC146657.1. On Nov 22, 2018 this sequence version replaced NM_020770.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF263462.1, AB037740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000271636.12/ ENSP00000271636.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065821.1
CDS	144..3755
Translation	MEQAPNMAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGLERQLEEKTEECSRLQELLERRKGEAQQSNKELQNMKRLLDQGEDLRHGLETQVMELQNKLKHVQGPEPAKEVLLKDLLETRELLEEVLEGKQRVEEQLRLRERELTALKGALKEEVASRDQEVEHVRQQYQRDTEQLRRSMQDATQDHAVLEAERQKMSALVRGLQRELEETSEETGHWQSMFQKNKEDLRATKQELLQLRMEKEEMEEELGEKIEVLQRELEQARASAGDTRQVEVLKKELLRTQEELKELQAERQSQEVAGRHRDRELEKQLAVLRVEADRGRELEEQNLQLQKTLQQLRQDCEEASKAKMVAEAEATVLGQRRAAVETTLRETQEENDEFRRRILGLEQQLKETRGLVDGGEAVEARLRDKLQRLEAEKQQLEEALNASQEEEGSLAAAKRALEARLEEAQRGLARLGQEQQTLNRALEEEGKQREVLRRGKAELEEQKRLLDRTVDRLNKELEKIGEDSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDEIQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEEKVSRLETELDEEKNTVELLTDRVNRGRDQVDQLRTELMQERSARQDLECDKISLERQNKDLKTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKTVLQSTNRKLERKVKELSIQIEDERQHVNDQKDQLSLRVKALKRQVDEAEEEIERLDGLRKKAQREVEEQHEVNEQLQARIKSLEKDSWRKASRSAAESALKNEGLSSDEEFDSVYDPSSIASLLTESNLQTSSC

Target ORF information:

RefSeq Version	NM_020770.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cingulin (CGN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020770.3

ORF Insert Sequence:

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGN ( XM_005245365.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245422.1 Homo sapiens cingulin (CGN), transcript variant X1, mRNA.

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CloneID	OHu31771
Clone ID Related Accession (Same CDS sequence)	XM_005245365.4 , XM_005245365.5 , NM_020770.2 , NM_020770.3
Accession Version	XM_005245365.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cingulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245365.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245422.1
CDS	157..3768
Translation	MEQAPNMAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGLERQLEEKTEECSRLQELLERRKGEAQQSNKELQNMKRLLDQGEDLRHGLETQVMELQNKLKHVQGPEPAKEVLLKDLLETRELLEEVLEGKQRVEEQLRLRERELTALKGALKEEVASRDQEVEHVRQQYQRDTEQLRRSMQDATQDHAVLEAERQKMSALVRGLQRELEETSEETGHWQSMFQKNKEDLRATKQELLQLRMEKEEMEEELGEKIEVLQRELEQARASAGDTRQVEVLKKELLRTQEELKELQAERQSQEVAGRHRDRELEKQLAVLRVEADRGRELEEQNLQLQKTLQQLRQDCEEASKAKMVAEAEATVLGQRRAAVETTLRETQEENDEFRRRILGLEQQLKETRGLVDGGEAVEARLRDKLQRLEAEKQQLEEALNASQEEEGSLAAAKRALEARLEEAQRGLARLGQEQQTLNRALEEEGKQREVLRRGKAELEEQKRLLDRTVDRLNKELEKIGEDSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDEIQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEEKVSRLETELDEEKNTVELLTDRVNRGRDQVDQLRTELMQERSARQDLECDKISLERQNKDLKTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKTVLQSTNRKLERKVKELSIQIEDERQHVNDQKDQLSLRVKALKRQVDEAEEEIERLDGLRKKAQREVEEQHEVNEQLQARIKSLEKDSWRKASRSAAESALKNEGLSSDEEFDSVYDPSSIASLLTESNLQTSSC

Target ORF information:

RefSeq Version	XM_005245365.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cingulin (CGN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245365.4

ORF Insert Sequence:

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CGN ( XM_005245365.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245422.1 Homo sapiens cingulin (CGN), transcript variant X1, mRNA.

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CloneID	OHu31771
Clone ID Related Accession (Same CDS sequence)	XM_005245365.4 , XM_005245365.5 , NM_020770.2 , NM_020770.3
Accession Version	XM_005245365.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cingulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245365.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245422.1
CDS	158..3769
Translation	MEQAPNMAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGLERQLEEKTEECSRLQELLERRKGEAQQSNKELQNMKRLLDQGEDLRHGLETQVMELQNKLKHVQGPEPAKEVLLKDLLETRELLEEVLEGKQRVEEQLRLRERELTALKGALKEEVASRDQEVEHVRQQYQRDTEQLRRSMQDATQDHAVLEAERQKMSALVRGLQRELEETSEETGHWQSMFQKNKEDLRATKQELLQLRMEKEEMEEELGEKIEVLQRELEQARASAGDTRQVEVLKKELLRTQEELKELQAERQSQEVAGRHRDRELEKQLAVLRVEADRGRELEEQNLQLQKTLQQLRQDCEEASKAKMVAEAEATVLGQRRAAVETTLRETQEENDEFRRRILGLEQQLKETRGLVDGGEAVEARLRDKLQRLEAEKQQLEEALNASQEEEGSLAAAKRALEARLEEAQRGLARLGQEQQTLNRALEEEGKQREVLRRGKAELEEQKRLLDRTVDRLNKELEKIGEDSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDEIQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEEKVSRLETELDEEKNTVELLTDRVNRGRDQVDQLRTELMQERSARQDLECDKISLERQNKDLKTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKTVLQSTNRKLERKVKELSIQIEDERQHVNDQKDQLSLRVKALKRQVDEAEEEIERLDGLRKKAQREVEEQHEVNEQLQARIKSLEKDSWRKASRSAAESALKNEGLSSDEEFDSVYDPSSIASLLTESNLQTSSC

Target ORF information:

RefSeq Version	XM_005245365.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cingulin (CGN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245365.5

ORF Insert Sequence:

ATGGAGCAGG CACCCAACAT GGCTGAGCCC CGGGGCCCCG TAGACCATGG AGTCCAGATT 
CGCTTCATCA CAGAGCCAGT GAGTGGTGCA GAGATGGGCA CTCTACGTCG AGGTGGACGA 
CGCCCAGCTA AGGATGCAAG AGCCAGTACC TACGGGGTTG CTGTGCGTGT GCAGGGAATC 
GCTGGGCAGC CCTTTGTGGT GCTCAACAGT GGGGAGAAAG GCGGTGACTC CTTTGGGGTC 
CAAATCAAGG GGGCCAATGA CCAAGGGGCC TCAGGAGCTC TGAGCTCAGA TTTGGAACTC 
CCTGAGAACC CCTACTCTCA GGTCAAGGGA TTTCCTGCCC CCTCGCAGAG CAGCACATCT 
GATGAGGAGC CTGGGGCCTA CTGGAATGGA AAGCTACTCC GTTCCCACTC CCAGGCCTCA 
CTGGCAGGCC CTGGCCCAGT GGATCCTAGT AACAGAAGCA ACAGCATGCT GGAGCTAGCC 
CCGAAAGTGG CTTCCCCAGG TAGCACCATT GACACTGCTC CCCTGTCTTC AGTGGACTCA 
CTCATCAACA AGTTTGACAG TCAACTTGGA GGCCAGGCCC GGGGTCGGAC TGGCCGCCGA 
ACACGGATGC TACCCCCTGA ACAGCGCAAA CGGAGCAAGA GCCTGGACAG CCGCCTCCCA 
CGGGACACCT TTGAGGAACG GGAGCGCCAG TCCACCAACC ACTGGACCTC TAGCACAAAA 
TATGACAACC ATGTGGGCAC TTCGAAGCAG CCAGCCCAGA GCCAGAACCT GAGTCCTCTC 
AGTGGCTTTA GCCGTTCTCG TCAGACTCAG GACTGGGTCC TTCAGAGTTT TGAGGAGCCG 
CGGAGGAGTG CACAGGACCC CACCATGCTG CAGTTCAAAT CAACTCCAGA CCTCCTTCGA 
GACCAGCAGG AGGCAGCCCC ACCAGGCAGT GTGGACCATA TGAAGGCCAC CATCTATGGC 
ATCCTGAGGG AGGGAAGCTC AGAAAGTGAA ACCTCTGTGA GGAGGAAGGT TAGTTTGGTG 
CTGGAGAAGA TGCAGCCTCT AGTGATGGTT TCTTCTGGTT CTACTAAGGC CGTGGCAGGG 
CAGGGTGAGC TTACCCGAAA AGTGGAGGAG CTACAGCGAA AGCTGGATGA AGAGGTGAAG 
AAGCGGCAGA AGCTAGAGCC ATCCCAAGTT GGGCTGGAGC GGCAGCTGGA GGAGAAAACA 
GAAGAGTGCA GCCGACTGCA GGAGCTGCTG GAGAGGAGGA AGGGGGAGGC CCAGCAGAGC 
AACAAGGAGC TCCAGAACAT GAAGCGCCTC TTGGACCAGG GTGAAGATTT ACGACATGGG 
CTGGAGACCC AGGTGATGGA GCTGCAGAAC AAGCTGAAAC ATGTCCAGGG TCCTGAGCCT 
GCTAAGGAGG TGTTACTGAA GGACCTGTTA GAGACCCGGG AACTTCTGGA AGAGGTCTTG 
GAGGGGAAAC AGCGAGTAGA GGAGCAGCTG AGGCTGCGGG AGCGGGAGTT GACAGCCCTG 
AAGGGGGCCC TGAAAGAGGA GGTAGCCTCC CGTGACCAGG AGGTGGAACA TGTCCGGCAG 
CAGTACCAGC GAGACACAGA GCAGCTCCGC AGGAGCATGC AAGATGCAAC CCAGGACCAT 
GCAGTGCTGG AGGCCGAGAG GCAGAAGATG TCAGCCCTTG TGCGAGGGCT GCAGAGGGAG 
CTGGAGGAGA CTTCAGAGGA GACAGGGCAT TGGCAGAGTA TGTTCCAGAA GAACAAGGAG 
GATCTTAGAG CCACCAAGCA GGAACTCCTG CAGCTGCGAA TGGAGAAGGA GGAGATGGAA 
GAGGAGCTTG GAGAGAAGAT AGAGGTCTTG CAGAGGGAAT TAGAGCAGGC CCGAGCTAGT 
GCTGGAGATA CTCGCCAGGT TGAGGTGCTC AAGAAGGAGC TGCTCCGGAC ACAGGAGGAG 
CTTAAGGAAC TGCAGGCAGA ACGGCAGAGC CAGGAGGTGG CTGGGCGACA CCGGGACCGG 
GAGTTGGAGA AGCAGCTGGC GGTCCTGAGG GTCGAGGCTG ATCGAGGTCG GGAGCTGGAA 
GAACAGAACC TCCAGCTACA AAAGACCCTC CAGCAACTGC GACAGGACTG TGAAGAGGCT 
TCCAAGGCTA AGATGGTGGC CGAGGCAGAG GCAACAGTGC TGGGGCAGCG GCGGGCCGCA 
GTGGAGACGA CGCTTCGGGA GACCCAGGAG GAAAATGACG AATTCCGCCG GCGCATCCTG 
GGTTTGGAGC AGCAGCTGAA GGAGACTCGA GGTCTGGTGG ATGGTGGGGA AGCGGTGGAG 
GCACGACTAC GGGACAAGCT GCAGCGGCTG GAGGCAGAGA AACAGCAGCT GGAGGAGGCC 
CTGAATGCGT CCCAGGAAGA GGAGGGGAGT CTGGCAGCAG CCAAGCGGGC ACTGGAGGCA 
CGCCTAGAGG AGGCTCAGCG GGGGCTGGCC CGCCTGGGGC AGGAGCAGCA GACACTGAAC 
CGGGCCCTGG AGGAGGAAGG GAAGCAGCGG GAGGTGCTCC GGCGAGGCAA GGCTGAGCTG 
GAGGAGCAGA AGCGTTTGCT GGACAGGACT GTGGACCGAC TGAACAAGGA GTTGGAGAAG 
ATCGGGGAGG ACTCTAAGCA AGCCCTGCAG CAGCTCCAGG CCCAGCTGGA GGATTATAAG 
GAAAAGGCCC GGCGGGAGGT GGCAGATGCC CAGCGCCAGG CCAAGGATTG GGCCAGTGAG 
GCTGAGAAGA CCTCTGGAGG ACTGAGCCGA CTTCAGGATG AGATCCAGAG GCTGCGGCAG 
GCCCTGCAGG CATCCCAGGC TGAGCGGGAC ACAGCCCGGC TGGACAAAGA GCTACTGGCC 
CAGCGACTGC AGGGGCTGGA GCAAGAGGCA GAGAACAAGA AGCGTTCCCA GGACGACAGG 
GCCCGGCAGC TGAAGGGTCT CGAGGAAAAA GTCTCACGGC TGGAAACAGA GTTAGATGAG 
GAGAAGAACA CCGTGGAGCT GCTAACAGAT CGGGTGAATC GTGGCCGGGA CCAGGTGGAT 
CAGCTGAGGA CAGAGCTCAT GCAGGAAAGG TCTGCTCGGC AGGACCTGGA GTGTGACAAA 
ATCTCCTTGG AGAGACAGAA CAAGGACCTG AAGACCCGGT TGGCCAGCTC AGAAGGCTTC 
CAGAAGCCTA GTGCCAGCCT CTCTCAGCTT GAGTCCCAGA ATCAGTTGTT GCAGGAGCGG 
CTACAGGCTG AAGAGAGGGA GAAGACAGTT CTGCAGTCTA CCAATCGAAA ACTGGAGCGG 
AAAGTTAAAG AACTATCCAT CCAGATTGAA GACGAGCGGC AGCATGTCAA TGACCAGAAA 
GACCAGCTAA GCCTGAGGGT GAAGGCTTTG AAGCGTCAGG TGGATGAAGC AGAAGAGGAA 
ATTGAGCGAC TGGACGGCCT GAGGAAGAAG GCCCAGCGTG AGGTGGAGGA GCAGCATGAG 
GTCAATGAAC AGCTCCAGGC CCGGATCAAG TCTCTGGAGA AGGACTCCTG GCGCAAAGCT 
TCCCGCTCAG CTGCTGAGTC AGCTCTCAAA AACGAAGGGC TGAGCTCAGA TGAGGAATTC 
GACAGTGTCT ACGATCCCTC GTCCATTGCA TCACTGCTTA CGGAGAGCAA CCTACAGACC 
AGCTCCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Role of Cingulin in Agonist-induced Vascular Endothelial Permeability.
The Journal of biological chemistry291(45)23681-23692(2016 Nov)
Tian Y,Gawlak G,Tian X,Shah AS,Sarich N,Citi S,Birukova AA

Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo.
Arteriosclerosis, thrombosis, and vascular biology36(4)647-54(2016 Apr)
Schossleitner K,Rauscher S,Gr?ger M,Friedl HP,Finsterwalder R,Habertheuer A,Sibilia M,Brostjan C,F?dinger D,Citi S,Petzelbauer P

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.
Molecular biology of the cell25(13)1995-2005(2014 Jul)
Guillemot L,Guerrera D,Spadaro D,Tapia R,Jond L,Citi S

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CGN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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