Fis1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fis1
Entrez Gene ID	66437
Full Name	fission, mitochondrial 1
Synonyms	2010003O14Rik,Ttc11
General protein information	Preferred Names fission, mitochondrial 1 Names mitochondrial fission 1 protein FIS1 homolog TPR repeat protein 11 fission 1 (mitochondrial outer membrane) homolog tetratricopeptide repeat domain 11 tetratricopeptide repeat protein 11
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001347504.1	NP_001334433.1	mitochondrial fission 1 protein isoform 3
NM_001163243.1	NP_001156715.1	mitochondrial fission 1 protein isoform 2
NM_025562.3	NP_079838.1	mitochondrial fission 1 protein isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FIS1	XP_001143081.2
Bos taurus (cattle)	FIS1	NP_001029956.1
Danio rerio (zebrafish)	im:6905231	XP_005172294.1
Caenorhabditis elegans (roundworm)	fis-2	NP_001024560.1
Xenopus tropicalis (tropical clawed frog)	fis1	NP_001123678.1
Canis lupus familiaris (dog)	FIS1	XP_536854.1
Homo sapiens (human)	FIS1	NP_057152.2
	FIS1	XP_001108062.1
Mus musculus (house mouse)	Fis1	NP_079838.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009852	XP_318964.4
Drosophila melanogaster (fruit fly)	Fis1	NP_001137607.1
Rattus norvegicus (Norway rat)	Fis1	NP_001099389.1

Gene Ontology
Bibliography

Related articles in PubMed

Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1.
Rojansky R, Cha MY, Chan DC
eLife5(2016 11)

Precise expression of Fis1 is important for glucose responsiveness of beta cells.
Schultz J, Waterstradt R, Kantowski T, Rickmann A, Reinhardt F, Sharoyko V, Mulder H, Tiedge M, Baltrusch S
The Journal of endocrinology230(1)81-91(2016 Jul)

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
G?mez-Del Arco P, Perdiguero E, Yunes-Leites PS, Ac?n-P?rez R, Zeini M, Garcia-Gomez A, Sreenivasan K, Jim?nez-Alc?zar M, Segal?s J, L?pez-Maderuelo D, Orn?s B, Jim?nez-Borreguero LJ, D'Amato G, Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T, de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Mu?oz-C?noves P, Redondo JM
Cell metabolism23(5)881-92(2016 May)

Adult mice expressing a Braf Q241R mutation on an ICR/CD-1 background exhibit a cardio-facio-cutaneous syndrome phenotype.
Moriya M, Inoue S, Miyagawa-Tomita S, Nakashima Y, Oba D, Niihori T, Hashi M, Ohnishi H, Kure S, Matsubara Y, Aoki Y
Human molecular genetics24(25)7349-60(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data confirm that FIS1 is a key regulator in pancreatic beta cells, because both glucose-stimulated insulin secretion and mitochondrial dynamics were clearly adapted to precise expression levels of this fission protein.
Title: Precise expression of Fis1 is important for glucose responsiveness of beta cells.

MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and suggest that they provide specificity to the division of mitochondria.
Title: Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission.

we find that either MiD49 or MiD51 can mediate Drp1 recruitment and mitochondrial fission in the absence of Fis1 and Mff
Title: Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission.

miR-484 attenuate Fis1 and mitochondrial fission by binding to N-terminal of Fis1 and inhibiting its translation.
Title: miR-484 regulates mitochondrial network through targeting Fis1.

study provides the first evidence that homocysteine-induced ganglion cell loss involves the dysregulation of mitochondrial dynamics, both in vivo and in vitro.
Title: Homocysteine-mediated modulation of mitochondrial dynamics in retinal ganglion cells.

The following Fis1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fis1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13241	NM_001163243.1 Latest version!	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu36186	NM_001347504.1 Latest version!	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13176	NM_025562.3 Latest version!	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fis1 ( NM_001163243.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156715.1 Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13241
Clone ID Related Accession (Same CDS sequence)	NM_001163243.1
Accession Version	NM_001163243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Mus musculus(house mouse)
Product	mitochondrial fission 1 protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA322565.1, AK008078.1 and CD774298.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF583067.1, BI412194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGAG ACGAAGCTGC AAGGAATTTT GAAAGGAAAT TTCAGTCTGA GCAGGCAGCT 
GGTTCTGTGT CCAAGAGCAC GCAATTTGAA TATGCCTGGT GCCTGGTTCG AAGCAAATAC 
AATGAGGACA TCCGCAGAGG CATCGTGCTG CTGGAGGAGC TGTTGCCCAA AGGGAGCAAA 
GAGGAACAGC GGGACTATGT CTTCTACCTG GCCGTGGGCA ACTACCGGCT CAAGGAATAT 
GAAAAGGCTC TAAAGTATGT GCGAGGGCTG TTGCAGACTG AGCCCCAGAA CAACCAGGCC 
AAGGAGCTGG AACGCCTGAT TGATAAGGCC ATGAAGAAAG ATGGACTGGT AGGCATGGCC 
ATCGTTGGTG GCATGGCCCT GGGCGTGGCA GGCCTGGCTG GACTCATTGG ACTGGCTGTC 
TCCAAGTCCA AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156715.1
CDS	293..730
Translation	MPRDEAARNFERKFQSEQAAGSVSKSTQFEYAWCLVRSKYNEDIRRGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS

Target ORF information:

RefSeq Version	NM_001163243.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163243.1

ORF Insert Sequence:

ATGCCCAGAG ACGAAGCTGC AAGGAATTTT GAAAGGAAAT TTCAGTCTGA GCAGGCAGCT 
GGTTCTGTGT CCAAGAGCAC GCAATTTGAA TATGCCTGGT GCCTGGTTCG AAGCAAATAC 
AATGAGGACA TCCGCAGAGG CATCGTGCTG CTGGAGGAGC TGTTGCCCAA AGGGAGCAAA 
GAGGAACAGC GGGACTATGT CTTCTACCTG GCCGTGGGCA ACTACCGGCT CAAGGAATAT 
GAAAAGGCTC TAAAGTATGT GCGAGGGCTG TTGCAGACTG AGCCCCAGAA CAACCAGGCC 
AAGGAGCTGG AACGCCTGAT TGATAAGGCC ATGAAGAAAG ATGGACTGGT AGGCATGGCC 
ATCGTTGGTG GCATGGCCCT GGGCGTGGCA GGCCTGGCTG GACTCATTGG ACTGGCTGTC 
TCCAAGTCCA AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_001347504.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334433.1 Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu36186
Clone ID Related Accession (Same CDS sequence)	NM_001347504.1
Accession Version	NM_001347504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Mus musculus(house mouse)
Product	mitochondrial fission 1 protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147987.5. On Nov 30, 2016 this sequence version replaced XM_006504489.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AV505347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATGCCC AGAGACGAAG CTGCAAGGAG CTGTTGCCCA AAGGGAGCAA AGAGGAACAG 
CGGGACTATG TCTTCTACCT GGCCGTGGGC AACTACCGGC TCAAGGAATA TGAAAAGGCT 
CTAAAGTATG TGCGAGGGCT GTTGCAGACT GAGCCCCAGA ACAACCAGGC CAAGGAGCTG 
GAACGCCTGA TTGATAAGGC CATGAAGAAA GATGGACTGG TAGGCATGGC CATCGTTGGT 
GGCATGGCCC TGGGCGTGGC AGGCCTGGCT GGACTCATTG GACTGGCTGT CTCCAAGTCC 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334433.1
CDS	289..597
Translation	MDAQRRSCKELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS

Target ORF information:

RefSeq Version	NM_001347504.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347504.1

ORF Insert Sequence:

ATGGATGCCC AGAGACGAAG CTGCAAGGAG CTGTTGCCCA AAGGGAGCAA AGAGGAACAG 
CGGGACTATG TCTTCTACCT GGCCGTGGGC AACTACCGGC TCAAGGAATA TGAAAAGGCT 
CTAAAGTATG TGCGAGGGCT GTTGCAGACT GAGCCCCAGA ACAACCAGGC CAAGGAGCTG 
GAACGCCTGA TTGATAAGGC CATGAAGAAA GATGGACTGG TAGGCATGGC CATCGTTGGT 
GGCATGGCCC TGGGCGTGGC AGGCCTGGCT GGACTCATTG GACTGGCTGT CTCCAAGTCC 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_025562.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079838.1 Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13176
Clone ID Related Accession (Same CDS sequence)	NM_025562.3
Accession Version	NM_025562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mitochondrial fission 1 protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW988976.1, AK008078.1 and CD774298.1. On Jul 11, 2009 this sequence version replaced NM_025562.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010783.1, AK008078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG TGCTGAACGA GCTGGTGTCT GTGGAGGATC TGAAGAATTT TGAAAGGAAA 
TTTCAGTCTG AGCAGGCAGC TGGTTCTGTG TCCAAGAGCA CGCAATTTGA ATATGCCTGG 
TGCCTGGTTC GAAGCAAATA CAATGAGGAC ATCCGCAGAG GCATCGTGCT GCTGGAGGAG 
CTGTTGCCCA AAGGGAGCAA AGAGGAACAG CGGGACTATG TCTTCTACCT GGCCGTGGGC 
AACTACCGGC TCAAGGAATA TGAAAAGGCT CTAAAGTATG TGCGAGGGCT GTTGCAGACT 
GAGCCCCAGA ACAACCAGGC CAAGGAGCTG GAACGCCTGA TTGATAAGGC CATGAAGAAA 
GATGGACTGG TAGGCATGGC CATCGTTGGT GGCATGGCCC TGGGCGTGGC AGGCCTGGCT 
GGACTCATTG GACTGGCTGT CTCCAAGTCC AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079838.1
CDS	108..566
Translation	MEAVLNELVSVEDLKNFERKFQSEQAAGSVSKSTQFEYAWCLVRSKYNEDIRRGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS

Target ORF information:

RefSeq Version	NM_025562.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus fission, mitochondrial 1 (Fis1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025562.3

ORF Insert Sequence:

ATGGAGGCCG TGCTGAACGA GCTGGTGTCT GTGGAGGATC TGAAGAATTT TGAAAGGAAA 
TTTCAGTCTG AGCAGGCAGC TGGTTCTGTG TCCAAGAGCA CGCAATTTGA ATATGCCTGG 
TGCCTGGTTC GAAGCAAATA CAATGAGGAC ATCCGCAGAG GCATCGTGCT GCTGGAGGAG 
CTGTTGCCCA AAGGGAGCAA AGAGGAACAG CGGGACTATG TCTTCTACCT GGCCGTGGGC 
AACTACCGGC TCAAGGAATA TGAAAAGGCT CTAAAGTATG TGCGAGGGCT GTTGCAGACT 
GAGCCCCAGA ACAACCAGGC CAAGGAGCTG GAACGCCTGA TTGATAAGGC CATGAAGAAA 
GATGGACTGG TAGGCATGGC CATCGTTGGT GGCATGGCCC TGGGCGTGGC AGGCCTGGCT 
GGACTCATTG GACTGGCTGT CTCCAAGTCC AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1.
eLife5(2016 11)
Rojansky R,Cha MY,Chan DC

Precise expression of Fis1 is important for glucose responsiveness of beta cells.
The Journal of endocrinology230(1)81-91(2016 Jul)
Schultz J,Waterstradt R,Kantowski T,Rickmann A,Reinhardt F,Sharoyko V,Mulder H,Tiedge M,Baltrusch S

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
Cell metabolism23(5)881-92(2016 May)
G?mez-Del Arco P,Perdiguero E,Yunes-Leites PS,Ac?n-P?rez R,Zeini M,Garcia-Gomez A,Sreenivasan K,Jim?nez-Alc?zar M,Segal?s J,L?pez-Maderuelo D,Orn?s B,Jim?nez-Borreguero LJ,D'Amato G,Enshell-Seijffers D,Morgan B,Georgopoulos K,Islam AB,Braun T,de la Pompa JL,Kim J,Enriquez JA,Ballestar E,Mu?oz-C?noves P,Redondo JM

Adult mice expressing a Braf Q241R mutation on an ICR/CD-1 background exhibit a cardio-facio-cutaneous syndrome phenotype.
Human molecular genetics24(25)7349-60(2015 Dec)
Moriya M,Inoue S,Miyagawa-Tomita S,Nakashima Y,Oba D,Niihori T,Hashi M,Ohnishi H,Kure S,Matsubara Y,Aoki Y

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Fis1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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