Tsc1 cDNA ORF clone, Mus musculus(house mouse)

The following Tsc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu23182 XM_030251935.1
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu23182 XM_011239144.2
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu23182 XM_030251936.1
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu29667 XM_006498222.4
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu29667 XM_006498221.4
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu29667 XM_011239143.3
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu29667 XM_011239142.3
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu17731 NM_001289575.1
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu23180 NM_001289576.1
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu23182 NM_022887.4
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu29667 XM_006498220.2
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu29668 XM_006498223.3
Latest version!
Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OMu29667 XM_011239142.2 Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu23182 XM_011239144.1 Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu29667 XM_011239143.2 Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu29667 XM_006498221.3 Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu29667 XM_006498222.3 Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu23182
    Clone ID Related Accession (Same CDS sequence) XM_030251936.1 , XM_011239144.1 , XM_030251935.1 , NM_022887.4 , XM_011239144.2
    Accession Version XM_030251936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107796.1
    CDS508..3990
    Translation

    Target ORF information:

    RefSeq Version XM_030251936.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23182
    Clone ID Related Accession (Same CDS sequence) XM_030251936.1 , XM_011239144.1 , XM_030251935.1 , NM_022887.4 , XM_011239144.2
    Accession Version XM_030251935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107795.1
    CDS477..3959
    Translation

    Target ORF information:

    RefSeq Version XM_030251935.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23182
    Clone ID Related Accession (Same CDS sequence) XM_030251936.1 , XM_011239144.1 , XM_030251935.1 , NM_022887.4 , XM_011239144.2
    Accession Version XM_011239144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237446.1
    CDS471..3953
    Translation

    Target ORF information:

    RefSeq Version XM_011239144.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_006498221.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498284.1
    CDS442..3966
    Translation

    Target ORF information:

    RefSeq Version XM_006498221.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498221.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_011239142.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237444.1
    CDS459..3983
    Translation

    Target ORF information:

    RefSeq Version XM_011239142.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239142.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_011239143.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237445.1
    CDS221..3745
    Translation

    Target ORF information:

    RefSeq Version XM_011239143.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239143.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_006498222.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498222.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498285.1
    CDS477..4001
    Translation

    Target ORF information:

    RefSeq Version XM_006498222.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498222.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17731
    Clone ID Related Accession (Same CDS sequence) NM_001289575.1
    Accession Version NM_001289575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Mus musculus(house mouse)
    Product hamartin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY275738.1, AJ271912.1, AK147428.1 and AI837319.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ271912.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GCAGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCTCC AGCCCCACCT
    TGTCCCCAAG ATGACTGTGT GCATGGTTCA GCAGCCCAGG CCTCAGCCAC AGCCCCCAGG
    AAGGAAGAAA GAGCAGATTC CTCAAGGCCT TACCTTCACA GACAGTCAAA CGACCGAGGA
    TTAGAGGATC CACCTGGAAG CAAAGGTTCC GTTACTCTGA GGAATCTACC TGATTTCCTA
    GGTGATCTGG CTTCTGAGGA AGACAGTATC GAGAAAGATA AGGAAGAAGC TGCAATATCT
    AAAGAGCTTT CTGAGATCAC TACTGCAGAG GCGGATCCTG TAGTTCCTCG AGGGGGCTTT
    GACTCTCCCT TCTACCGAGA CAGTCTCTCT GGCTCTCAGC GGAAGACTCA TTCGGCAGCC
    TCTGGGACTC AGGGCTCCAG CGTGAACCCT GAGCCTTTGC ACTCCTCCCT GGACAAACAT
    GGGCCTGACA CACCAAAGCA AGCCTTTACT CCCATAGACC CACCCTCTGG CAGTGCTGAT
    GTCAGTCCCG CTGGGGACAG GGATCGCCAG ACTTCTCTGG AGACCAGTAT CCTCACTCCC
    AGCCCTTGCA AAATCCCACC TCAGAGGGGA GTGAGCTTTG GAAGTGGGCA GCTTCCCCCA
    TATGATCATC TCTTTGAGGT GGCCTTGCCA AAGACTGCCT GTCACTTTGT CAGCAAGAAG
    ACTGAGGAGC TGTTGAAGAA AGTGAAAGGA AACCCTGAGG AAGACTGTGT GCCCTCTACC
    TCCCCAATGG AAGTACTGGA CAGACTGATA GAGCAGGGAG CAGGTGCGCA CAGCAAGGAG
    CTGAGCAGGT TGTCCCTGCC CAGCAAGTCT GTTGACTGGA CCCACTTTGG AGGCTCTCCT
    CCCTCAGATG AGCTCCGGAC CCTCCGAGAC CAGTTGCTTT TACTGCACAA TCAGCTGCTG
    TACGAGCGCT TCAAGCGGCA GCAGCATGCA CTCAGGAACA GAAGGCTCCT GCGCAAGGTG
    ATCCGAGCAG CGGCTCTGGA GGAACACAAT GCAGCAATGA AAGATCAGTT GAAGTTACAA
    GAGAAGGACA TACAGATGTG GAAGGTGAGT CTGCAGAAAG AACAAGCCCG ATACAGTCAG
    CTTCAGGAAC AACGTGACAC CATGGTGACC CAACTGCATA GCCAGATCAG ACAGCTACAG
    CATGACCGAG AAGAATTCTA CAACCAGAGT CAGGAGTTAC AGACAAAGCT GGAGGACTGC
    AGAAACATGA TTGCGGAGCT TCGGGTGGAG CTGAAGAAGG CTAACAACAA GGTGTGCCAC
    ACTGAGCTGC TGCTCAGCCA GGTCTCTCAG AAGCTCTCCA ACAGTGAGTC AGTGCAGCAG
    CAGATGGAGT TCTTGAATAG GCAGCTCCTG GTGCTCGGGG AGGTCAATGA GCTGTACCTG
    GAGCAGCTGC AGAGCAAGCA TCCTGACACC ACCAAGGAAG TAGAAATGAT GAAAACTGCA
    TATCGGAAAG AGCTAGAGAA AAACAGAAGC CACCTTCTCC AGCAGAACCA AAGGTTGGAC
    GCCTCACAGA GGCGAGTTTT GGAACTGGAG TCTCTTCTGG CCAAGAAAGA CCACCTTCTC
    CTAGAACAGA AGAAATATCT TGAGGATGTC AAGAGCCAGG CGAGTGGACA GCTGCTGGCT
    GCAGAGAGCA GGTATGAGGC TCAGAGAAAG ATCACCCGGG TGTTGGAACT GGAGATCCTA
    GACTTGTATG GCAGGTTGGA AAAAGATGGC CGCCTACGGA AACTAGAAGA GGACAGAGCA
    GAGGCAGCAG AGGCAGCAGA AGAGAGGCTT GACTGTTGTA GTGATGGATG CACAGATTCC
    TTGGTAGGAC ATAATGAAGA GGCTTCTGGT CACAATGGTG AGACCAGGAC CTCCAGACCT
    GGTGGCACCC GGGCCAGCTG TGGAGGTAGA GTCACTGGAG GCAGCAGCAG CAGCAGCAGT
    GAGCTTTCCA CTCCAGAGAA ACCCCCGAGC CAGAGGTTCA GCAGCCGGTG GGAACCTGCC
    CTGGGCGAGC CCTCCAGCAG CATCCCCACC ACTGTTGGCT CACTTCCCAG TTCCAAAAGC
    TTCCTGGGCA TGAAGGCCCG GGAGCTGTTC CGTAATAAGA GCGAGAGCCA GTGTGATGAG
    GACAGCGTGA CCATGAGTAG CAGCAGCCTT TCTGAGACCC TGAAGACAGA ACTGGGCAAG
    GACTCGGGCA CAGAAAACAA GACTTCCCTG AGTCTAGATG CCCCACACCC ATCTTCCCCA
    AACTCAGACA ATGTGGGGCA GCTCCACATC ATGGACTACA ATGAGACTCA TCCTGAACAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276504.1
    CDS231..3716
    Translation

    Target ORF information:

    RefSeq Version NM_001289575.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GCAGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCTCC AGCCCCACCT
    TGTCCCCAAG ATGACTGTGT GCATGGTTCA GCAGCCCAGG CCTCAGCCAC AGCCCCCAGG
    AAGGAAGAAA GAGCAGATTC CTCAAGGCCT TACCTTCACA GACAGTCAAA CGACCGAGGA
    TTAGAGGATC CACCTGGAAG CAAAGGTTCC GTTACTCTGA GGAATCTACC TGATTTCCTA
    GGTGATCTGG CTTCTGAGGA AGACAGTATC GAGAAAGATA AGGAAGAAGC TGCAATATCT
    AAAGAGCTTT CTGAGATCAC TACTGCAGAG GCGGATCCTG TAGTTCCTCG AGGGGGCTTT
    GACTCTCCCT TCTACCGAGA CAGTCTCTCT GGCTCTCAGC GGAAGACTCA TTCGGCAGCC
    TCTGGGACTC AGGGCTCCAG CGTGAACCCT GAGCCTTTGC ACTCCTCCCT GGACAAACAT
    GGGCCTGACA CACCAAAGCA AGCCTTTACT CCCATAGACC CACCCTCTGG CAGTGCTGAT
    GTCAGTCCCG CTGGGGACAG GGATCGCCAG ACTTCTCTGG AGACCAGTAT CCTCACTCCC
    AGCCCTTGCA AAATCCCACC TCAGAGGGGA GTGAGCTTTG GAAGTGGGCA GCTTCCCCCA
    TATGATCATC TCTTTGAGGT GGCCTTGCCA AAGACTGCCT GTCACTTTGT CAGCAAGAAG
    ACTGAGGAGC TGTTGAAGAA AGTGAAAGGA AACCCTGAGG AAGACTGTGT GCCCTCTACC
    TCCCCAATGG AAGTACTGGA CAGACTGATA GAGCAGGGAG CAGGTGCGCA CAGCAAGGAG
    CTGAGCAGGT TGTCCCTGCC CAGCAAGTCT GTTGACTGGA CCCACTTTGG AGGCTCTCCT
    CCCTCAGATG AGCTCCGGAC CCTCCGAGAC CAGTTGCTTT TACTGCACAA TCAGCTGCTG
    TACGAGCGCT TCAAGCGGCA GCAGCATGCA CTCAGGAACA GAAGGCTCCT GCGCAAGGTG
    ATCCGAGCAG CGGCTCTGGA GGAACACAAT GCAGCAATGA AAGATCAGTT GAAGTTACAA
    GAGAAGGACA TACAGATGTG GAAGGTGAGT CTGCAGAAAG AACAAGCCCG ATACAGTCAG
    CTTCAGGAAC AACGTGACAC CATGGTGACC CAACTGCATA GCCAGATCAG ACAGCTACAG
    CATGACCGAG AAGAATTCTA CAACCAGAGT CAGGAGTTAC AGACAAAGCT GGAGGACTGC
    AGAAACATGA TTGCGGAGCT TCGGGTGGAG CTGAAGAAGG CTAACAACAA GGTGTGCCAC
    ACTGAGCTGC TGCTCAGCCA GGTCTCTCAG AAGCTCTCCA ACAGTGAGTC AGTGCAGCAG
    CAGATGGAGT TCTTGAATAG GCAGCTCCTG GTGCTCGGGG AGGTCAATGA GCTGTACCTG
    GAGCAGCTGC AGAGCAAGCA TCCTGACACC ACCAAGGAAG TAGAAATGAT GAAAACTGCA
    TATCGGAAAG AGCTAGAGAA AAACAGAAGC CACCTTCTCC AGCAGAACCA AAGGTTGGAC
    GCCTCACAGA GGCGAGTTTT GGAACTGGAG TCTCTTCTGG CCAAGAAAGA CCACCTTCTC
    CTAGAACAGA AGAAATATCT TGAGGATGTC AAGAGCCAGG CGAGTGGACA GCTGCTGGCT
    GCAGAGAGCA GGTATGAGGC TCAGAGAAAG ATCACCCGGG TGTTGGAACT GGAGATCCTA
    GACTTGTATG GCAGGTTGGA AAAAGATGGC CGCCTACGGA AACTAGAAGA GGACAGAGCA
    GAGGCAGCAG AGGCAGCAGA AGAGAGGCTT GACTGTTGTA GTGATGGATG CACAGATTCC
    TTGGTAGGAC ATAATGAAGA GGCTTCTGGT CACAATGGTG AGACCAGGAC CTCCAGACCT
    GGTGGCACCC GGGCCAGCTG TGGAGGTAGA GTCACTGGAG GCAGCAGCAG CAGCAGCAGT
    GAGCTTTCCA CTCCAGAGAA ACCCCCGAGC CAGAGGTTCA GCAGCCGGTG GGAACCTGCC
    CTGGGCGAGC CCTCCAGCAG CATCCCCACC ACTGTTGGCT CACTTCCCAG TTCCAAAAGC
    TTCCTGGGCA TGAAGGCCCG GGAGCTGTTC CGTAATAAGA GCGAGAGCCA GTGTGATGAG
    GACAGCGTGA CCATGAGTAG CAGCAGCCTT TCTGAGACCC TGAAGACAGA ACTGGGCAAG
    GACTCGGGCA CAGAAAACAA GACTTCCCTG AGTCTAGATG CCCCACACCC ATCTTCCCCA
    AACTCAGACA ATGTGGGGCA GCTCCACATC ATGGACTACA ATGAGACTCA TCCTGAACAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23180
    Clone ID Related Accession (Same CDS sequence) NM_001289576.1
    Accession Version NM_001289576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Mus musculus(house mouse)
    Product hamartin isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY275738.1, AK147428.1 and AI837319.1. On Jan 24, 2014 this sequence version replaced XM_006498225.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites, one each in the 5' and 3' coding regions, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGA TGAGCTCCGG
    ACCCTCCGAG ACCAGTTGCT TTTACTGCAC AATCAGCTGC TGTACGAGCG CTTCAAGCGG
    CAGCAGCATG CACTCAGGAA CAGAAGGCTC CTGCGCAAGG TGATCCGAGC AGCGGCTCTG
    GAGGAACACA ATGCAGCAAT GAAAGATCAG TTGAAGTTAC AAGAGAAGGA CATACAGATG
    TGGAAGGTGA GTCTGCAGAA AGAACAAGCC CGATACAGTC AGCTTCAGGA ACAACGTGAC
    ACCATGGTGA CCCAACTGCA TAGCCAGATC AGACAGCTAC AGCATGACCG AGAAGAATTC
    TACAACCAGA GTCAGGAGTT ACAGACAAAG CTGGAGGACT GCAGAAACAT GATTGCGGAG
    CTTCGGGTGG AGCTGAAGAA GGCTAACAAC AAGGTGTGCC ACACTGAGCT GCTGCTCAGC
    CAGGTCTCTC AGAAGCTCTC CAACAGTGAG TCAGTGCAGC AGCAGATGGA GTTCTTGAAT
    AGGCAGCTCC TGGTGCTCGG GGAGGTCAAT GAGCTGTACC TGGAGCAGCT GCAGAGCAAG
    CATCCTGACA CCACCAAGGA AGTAGAAATG ATGAAAACTG CATATCGGAA AGAGCTAGAG
    AAAAACAGAA GCCACCTTCT CCAGCAGAAC CAAAGGTTGG ACGCCTCACA GAGGCGAGTT
    TTGGAACTGG AGTCTCTTCT GGCCAAGAAA GACCACCTTC TCCTAGAACA GAAGAAATAT
    CTTGAGGATG TCAAGAGCCA GGCGAGTGGA CAGCTGCTGG CTGCAGAGAG CAGGTATGAG
    GCTCAGAGAA AGATCACCCG GGTGTTGGAA CTGGAGATCC TAGACTTGTA TGGCAGGTTG
    GAAAAAGATG GCCGCCTACG GAAACTAGAA GAGGACAGAG CAGAGGCAGC AGAGGCAGCA
    GAAGAGAGGC TTGACTGTTG TAGTGATGGA TGCACAGATT CCTTGGTAGG ACATAATGAA
    GAGGCTTCTG GTCACAATGG TGAGACCAGG ACCTCCAGAC CTGGTGGCAC CCGGGCCAGC
    TGTGGAGGTA GAGTCACTGG AGGCAGCAGC AGCAGCAGCA GTGAGCTTTC CACTCCAGAG
    AAACCCCCGA GCCAGAGGTT CAGCAGCCGG TGGGAACCTG CCCTGGGCGA GCCCTCCAGC
    AGCATCCCCA CCACTGTTGG CTCACTTCCC AGTTCCAAAA GCTTCCTGGG CATGAAGGCC
    CGGGAGCTGT TCCGTAATAA GAGCGAGAGC CAGTGTGATG AGGACAGCGT GACCATGAGT
    AGCAGCAGCC TTTCTGAGAC CCTGAAGACA GAACTGGGCA AGGACTCGGG CACAGAAAAC
    AAGACTTCCC TGAGTCTAGA TGCCCCACAC CCATCTTCCC CAAACTCAGA CAATGTGGGG
    CAGCTCCACA TCATGGACTA CAATGAGACT CATCCTGAAC ACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276505.1
    CDS231..3698
    Translation

    Target ORF information:

    RefSeq Version NM_001289576.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGA TGAGCTCCGG
    ACCCTCCGAG ACCAGTTGCT TTTACTGCAC AATCAGCTGC TGTACGAGCG CTTCAAGCGG
    CAGCAGCATG CACTCAGGAA CAGAAGGCTC CTGCGCAAGG TGATCCGAGC AGCGGCTCTG
    GAGGAACACA ATGCAGCAAT GAAAGATCAG TTGAAGTTAC AAGAGAAGGA CATACAGATG
    TGGAAGGTGA GTCTGCAGAA AGAACAAGCC CGATACAGTC AGCTTCAGGA ACAACGTGAC
    ACCATGGTGA CCCAACTGCA TAGCCAGATC AGACAGCTAC AGCATGACCG AGAAGAATTC
    TACAACCAGA GTCAGGAGTT ACAGACAAAG CTGGAGGACT GCAGAAACAT GATTGCGGAG
    CTTCGGGTGG AGCTGAAGAA GGCTAACAAC AAGGTGTGCC ACACTGAGCT GCTGCTCAGC
    CAGGTCTCTC AGAAGCTCTC CAACAGTGAG TCAGTGCAGC AGCAGATGGA GTTCTTGAAT
    AGGCAGCTCC TGGTGCTCGG GGAGGTCAAT GAGCTGTACC TGGAGCAGCT GCAGAGCAAG
    CATCCTGACA CCACCAAGGA AGTAGAAATG ATGAAAACTG CATATCGGAA AGAGCTAGAG
    AAAAACAGAA GCCACCTTCT CCAGCAGAAC CAAAGGTTGG ACGCCTCACA GAGGCGAGTT
    TTGGAACTGG AGTCTCTTCT GGCCAAGAAA GACCACCTTC TCCTAGAACA GAAGAAATAT
    CTTGAGGATG TCAAGAGCCA GGCGAGTGGA CAGCTGCTGG CTGCAGAGAG CAGGTATGAG
    GCTCAGAGAA AGATCACCCG GGTGTTGGAA CTGGAGATCC TAGACTTGTA TGGCAGGTTG
    GAAAAAGATG GCCGCCTACG GAAACTAGAA GAGGACAGAG CAGAGGCAGC AGAGGCAGCA
    GAAGAGAGGC TTGACTGTTG TAGTGATGGA TGCACAGATT CCTTGGTAGG ACATAATGAA
    GAGGCTTCTG GTCACAATGG TGAGACCAGG ACCTCCAGAC CTGGTGGCAC CCGGGCCAGC
    TGTGGAGGTA GAGTCACTGG AGGCAGCAGC AGCAGCAGCA GTGAGCTTTC CACTCCAGAG
    AAACCCCCGA GCCAGAGGTT CAGCAGCCGG TGGGAACCTG CCCTGGGCGA GCCCTCCAGC
    AGCATCCCCA CCACTGTTGG CTCACTTCCC AGTTCCAAAA GCTTCCTGGG CATGAAGGCC
    CGGGAGCTGT TCCGTAATAA GAGCGAGAGC CAGTGTGATG AGGACAGCGT GACCATGAGT
    AGCAGCAGCC TTTCTGAGAC CCTGAAGACA GAACTGGGCA AGGACTCGGG CACAGAAAAC
    AAGACTTCCC TGAGTCTAGA TGCCCCACAC CCATCTTCCC CAAACTCAGA CAATGTGGGG
    CAGCTCCACA TCATGGACTA CAATGAGACT CATCCTGAAC ACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23182
    Clone ID Related Accession (Same CDS sequence) XM_030251936.1 , XM_011239144.1 , XM_030251935.1 , NM_022887.4 , XM_011239144.2
    Accession Version NM_022887.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Mus musculus(house mouse)
    Product hamartin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY275738.1, AK030318.1, AJ271912.1, AK147428.1 and AI837319.1. On or before Jan 24, 2014 this sequence version replaced XM_006498224.1, NM_022887.3. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter that isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030318.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_075025.2
    CDS202..3684
    Translation

    Target ORF information:

    RefSeq Version NM_022887.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022887.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23182
    Clone ID Related Accession (Same CDS sequence) XM_030251936.1 , XM_011239144.1 , XM_030251935.1 , NM_022887.4 , XM_011239144.2
    Accession Version XM_011239144.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237446.1
    CDS246..3728
    Translation

    Target ORF information:

    RefSeq Version XM_011239144.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239144.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG CTAGTCTCTG GAGCCCATCT GCGGTCTGTG GTATGACCAC TCCTCCTACG
    TCTCCTGGAA ATGTCCCAGC TGATTTGTCA CATCCGTATA GTAAAGCCTT TGGTACCACT
    GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCTCCAGC CCCACCTTGT
    CCCCAAGATG ACTGTGTGCA TGGTTCAGCA GCCCAGGCCT CAGCCACAGC CCCCAGGAAG
    GAAGAAAGAG CAGATTCCTC AAGGCCTTAC CTTCACAGAC AGTCAAACGA CCGAGGATTA
    GAGGATCCAC CTGGAAGCAA AGGTTCCGTT ACTCTGAGGA ATCTACCTGA TTTCCTAGGT
    GATCTGGCTT CTGAGGAAGA CAGTATCGAG AAAGATAAGG AAGAAGCTGC AATATCTAAA
    GAGCTTTCTG AGATCACTAC TGCAGAGGCG GATCCTGTAG TTCCTCGAGG GGGCTTTGAC
    TCTCCCTTCT ACCGAGACAG TCTCTCTGGC TCTCAGCGGA AGACTCATTC GGCAGCCTCT
    GGGACTCAGG GCTCCAGCGT GAACCCTGAG CCTTTGCACT CCTCCCTGGA CAAACATGGG
    CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCCAC CCTCTGGCAG TGCTGATGTC
    AGTCCCGCTG GGGACAGGGA TCGCCAGACT TCTCTGGAGA CCAGTATCCT CACTCCCAGC
    CCTTGCAAAA TCCCACCTCA GAGGGGAGTG AGCTTTGGAA GTGGGCAGCT TCCCCCATAT
    GATCATCTCT TTGAGGTGGC CTTGCCAAAG ACTGCCTGTC ACTTTGTCAG CAAGAAGACT
    GAGGAGCTGT TGAAGAAAGT GAAAGGAAAC CCTGAGGAAG ACTGTGTGCC CTCTACCTCC
    CCAATGGAAG TACTGGACAG ACTGATAGAG CAGGGAGCAG GTGCGCACAG CAAGGAGCTG
    AGCAGGTTGT CCCTGCCCAG CAAGTCTGTT GACTGGACCC ACTTTGGAGG CTCTCCTCCC
    TCAGATGAGC TCCGGACCCT CCGAGACCAG TTGCTTTTAC TGCACAATCA GCTGCTGTAC
    GAGCGCTTCA AGCGGCAGCA GCATGCACTC AGGAACAGAA GGCTCCTGCG CAAGGTGATC
    CGAGCAGCGG CTCTGGAGGA ACACAATGCA GCAATGAAAG ATCAGTTGAA GTTACAAGAG
    AAGGACATAC AGATGTGGAA GGTGAGTCTG CAGAAAGAAC AAGCCCGATA CAGTCAGCTT
    CAGGAACAAC GTGACACCAT GGTGACCCAA CTGCATAGCC AGATCAGACA GCTACAGCAT
    GACCGAGAAG AATTCTACAA CCAGAGTCAG GAGTTACAGA CAAAGCTGGA GGACTGCAGA
    AACATGATTG CGGAGCTTCG GGTGGAGCTG AAGAAGGCTA ACAACAAGGT GTGCCACACT
    GAGCTGCTGC TCAGCCAGGT CTCTCAGAAG CTCTCCAACA GTGAGTCAGT GCAGCAGCAG
    ATGGAGTTCT TGAATAGGCA GCTCCTGGTG CTCGGGGAGG TCAATGAGCT GTACCTGGAG
    CAGCTGCAGA GCAAGCATCC TGACACCACC AAGGAAGTAG AAATGATGAA AACTGCATAT
    CGGAAAGAGC TAGAGAAAAA CAGAAGCCAC CTTCTCCAGC AGAACCAAAG GTTGGACGCC
    TCACAGAGGC GAGTTTTGGA ACTGGAGTCT CTTCTGGCCA AGAAAGACCA CCTTCTCCTA
    GAACAGAAGA AATATCTTGA GGATGTCAAG AGCCAGGCGA GTGGACAGCT GCTGGCTGCA
    GAGAGCAGGT ATGAGGCTCA GAGAAAGATC ACCCGGGTGT TGGAACTGGA GATCCTAGAC
    TTGTATGGCA GGTTGGAAAA AGATGGCCGC CTACGGAAAC TAGAAGAGGA CAGAGCAGAG
    GCAGCAGAGG CAGCAGAAGA GAGGCTTGAC TGTTGTAGTG ATGGATGCAC AGATTCCTTG
    GTAGGACATA ATGAAGAGGC TTCTGGTCAC AATGGTGAGA CCAGGACCTC CAGACCTGGT
    GGCACCCGGG CCAGCTGTGG AGGTAGAGTC ACTGGAGGCA GCAGCAGCAG CAGCAGTGAG
    CTTTCCACTC CAGAGAAACC CCCGAGCCAG AGGTTCAGCA GCCGGTGGGA ACCTGCCCTG
    GGCGAGCCCT CCAGCAGCAT CCCCACCACT GTTGGCTCAC TTCCCAGTTC CAAAAGCTTC
    CTGGGCATGA AGGCCCGGGA GCTGTTCCGT AATAAGAGCG AGAGCCAGTG TGATGAGGAC
    AGCGTGACCA TGAGTAGCAG CAGCCTTTCT GAGACCCTGA AGACAGAACT GGGCAAGGAC
    TCGGGCACAG AAAACAAGAC TTCCCTGAGT CTAGATGCCC CACACCCATC TTCCCCAAAC
    TCAGACAATG TGGGGCAGCT CCACATCATG GACTACAATG AGACTCATCC TGAACACAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_011239142.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237444.1
    CDS467..3991
    Translation

    Target ORF information:

    RefSeq Version XM_011239142.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_006498220.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498283.1
    CDS246..3770
    Translation

    Target ORF information:

    RefSeq Version XM_006498220.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498220.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_011239143.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237445.1
    CDS228..3752
    Translation

    Target ORF information:

    RefSeq Version XM_011239143.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_006498221.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498284.1
    CDS246..3770
    Translation

    Target ORF information:

    RefSeq Version XM_006498221.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498221.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29667
    Clone ID Related Accession (Same CDS sequence) XM_011239142.2 , XM_006498220.2 , XM_006498221.4 , XM_011239142.3 , XM_011239143.2 , XM_011239143.3 , XM_006498221.3 , XM_006498222.3 , XM_006498222.4
    Accession Version XM_006498222.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498285.1
    CDS420..3944
    Translation

    Target ORF information:

    RefSeq Version XM_006498222.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tuberous sclerosis 1 (Tsc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GGCTCTCCTC CCTCAGATGA GCTCCGGACC
    CTCCGAGACC AGTTGCTTTT ACTGCACAAT CAGCTGCTGT ACGAGCGCTT CAAGCGGCAG
    CAGCATGCAC TCAGGAACAG AAGGCTCCTG CGCAAGGTGA TCCGAGCAGC GGCTCTGGAG
    GAACACAATG CAGCAATGAA AGATCAGTTG AAGTTACAAG AGAAGGACAT ACAGATGTGG
    AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA TACAGTCAGC TTCAGGAACA ACGTGACACC
    ATGGTGACCC AACTGCATAG CCAGATCAGA CAGCTACAGC ATGACCGAGA AGAATTCTAC
    AACCAGAGTC AGGAGTTACA GACAAAGCTG GAGGACTGCA GAAACATGAT TGCGGAGCTT
    CGGGTGGAGC TGAAGAAGGC TAACAACAAG GTGTGCCACA CTGAGCTGCT GCTCAGCCAG
    GTCTCTCAGA AGCTCTCCAA CAGTGAGTCA GTGCAGCAGC AGATGGAGTT CTTGAATAGG
    CAGCTCCTGG TGCTCGGGGA GGTCAATGAG CTGTACCTGG AGCAGCTGCA GAGCAAGCAT
    CCTGACACCA CCAAGGAAGT AGAAATGATG AAAACTGCAT ATCGGAAAGA GCTAGAGAAA
    AACAGAAGCC ACCTTCTCCA GCAGAACCAA AGGTTGGACG CCTCACAGAG GCGAGTTTTG
    GAACTGGAGT CTCTTCTGGC CAAGAAAGAC CACCTTCTCC TAGAACAGAA GAAATATCTT
    GAGGATGTCA AGAGCCAGGC GAGTGGACAG CTGCTGGCTG CAGAGAGCAG GTATGAGGCT
    CAGAGAAAGA TCACCCGGGT GTTGGAACTG GAGATCCTAG ACTTGTATGG CAGGTTGGAA
    AAAGATGGCC GCCTACGGAA ACTAGAAGAG GACAGAGCAG AGGCAGCAGA GGCAGCAGAA
    GAGAGGCTTG ACTGTTGTAG TGATGGATGC ACAGATTCCT TGGTAGGACA TAATGAAGAG
    GCTTCTGGTC ACAATGGTGA GACCAGGACC TCCAGACCTG GTGGCACCCG GGCCAGCTGT
    GGAGGTAGAG TCACTGGAGG CAGCAGCAGC AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA
    CCCCCGAGCC AGAGGTTCAG CAGCCGGTGG GAACCTGCCC TGGGCGAGCC CTCCAGCAGC
    ATCCCCACCA CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG
    GAGCTGTTCC GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC
    AGCAGCCTTT CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG
    ACTTCCCTGA GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG
    CTCCACATCA TGGACTACAA TGAGACTCAT CCTGAACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29668
    Clone ID Related Accession (Same CDS sequence) XM_006498223.3
    Accession Version XM_006498223.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product hamartin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GATGAGCTCC GGACCCTCCG AGACCAGTTG
    CTTTTACTGC ACAATCAGCT GCTGTACGAG CGCTTCAAGC GGCAGCAGCA TGCACTCAGG
    AACAGAAGGC TCCTGCGCAA GGTGATCCGA GCAGCGGCTC TGGAGGAACA CAATGCAGCA
    ATGAAAGATC AGTTGAAGTT ACAAGAGAAG GACATACAGA TGTGGAAGGT GAGTCTGCAG
    AAAGAACAAG CCCGATACAG TCAGCTTCAG GAACAACGTG ACACCATGGT GACCCAACTG
    CATAGCCAGA TCAGACAGCT ACAGCATGAC CGAGAAGAAT TCTACAACCA GAGTCAGGAG
    TTACAGACAA AGCTGGAGGA CTGCAGAAAC ATGATTGCGG AGCTTCGGGT GGAGCTGAAG
    AAGGCTAACA ACAAGGTGTG CCACACTGAG CTGCTGCTCA GCCAGGTCTC TCAGAAGCTC
    TCCAACAGTG AGTCAGTGCA GCAGCAGATG GAGTTCTTGA ATAGGCAGCT CCTGGTGCTC
    GGGGAGGTCA ATGAGCTGTA CCTGGAGCAG CTGCAGAGCA AGCATCCTGA CACCACCAAG
    GAAGTAGAAA TGATGAAAAC TGCATATCGG AAAGAGCTAG AGAAAAACAG AAGCCACCTT
    CTCCAGCAGA ACCAAAGGTT GGACGCCTCA CAGAGGCGAG TTTTGGAACT GGAGTCTCTT
    CTGGCCAAGA AAGACCACCT TCTCCTAGAA CAGAAGAAAT ATCTTGAGGA TGTCAAGAGC
    CAGGCGAGTG GACAGCTGCT GGCTGCAGAG AGCAGGTATG AGGCTCAGAG AAAGATCACC
    CGGGTGTTGG AACTGGAGAT CCTAGACTTG TATGGCAGGT TGGAAAAAGA TGGCCGCCTA
    CGGAAACTAG AAGAGGACAG AGCAGAGGCA GCAGAGGCAG CAGAAGAGAG GCTTGACTGT
    TGTAGTGATG GATGCACAGA TTCCTTGGTA GGACATAATG AAGAGGCTTC TGGTCACAAT
    GGTGAGACCA GGACCTCCAG ACCTGGTGGC ACCCGGGCCA GCTGTGGAGG TAGAGTCACT
    GGAGGCAGCA GCAGCAGCAG CAGTGAGCTT TCCACTCCAG AGAAACCCCC GAGCCAGAGG
    TTCAGCAGCC GGTGGGAACC TGCCCTGGGC GAGCCCTCCA GCAGCATCCC CACCACTGTT
    GGCTCACTTC CCAGTTCCAA AAGCTTCCTG GGCATGAAGG CCCGGGAGCT GTTCCGTAAT
    AAGAGCGAGA GCCAGTGTGA TGAGGACAGC GTGACCATGA GTAGCAGCAG CCTTTCTGAG
    ACCCTGAAGA CAGAACTGGG CAAGGACTCG GGCACAGAAA ACAAGACTTC CCTGAGTCTA
    GATGCCCCAC ACCCATCTTC CCCAAACTCA GACAATGTGG GGCAGCTCCA CATCATGGAC
    TACAATGAGA CTCATCCTGA ACACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006498286.1
    CDS246..3755
    Translation

    Target ORF information:

    RefSeq Version XM_006498223.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TSC complex subunit 1 (Tsc1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006498223.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAGT TAGCCAACAT TGGGGAGCTG CTCTCCATGC TGGACTCCTC CACACTGGGT 
    GTGCGGGATG ACGTGACAGC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG
    CTTGTCAACA CGTTGGTTGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTGCACATC
    CTGACCACCT TGCAGGAGCC ACACGATAAG CACCTCTTGG ACAAAATTAA TGAGTATGTA
    GGCAAAGCTG CTACCCGTTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTATT GAAATGTCTC
    AAGATGGACA CTGATGTTGT GGTCCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTC
    CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT
    CTCTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTGTACCT GGTCCATCTT
    CATGCCAGTG TTTATGCCCT CTTTCACCGC CTTTATGGGA TGTACCCATG TAACTTCGTC
    TCCTTCCTGC GCTCTCACTA CAGTATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC
    AAAATCTCTC TGGACCCTAC AGAAGCCTCG TATGAAGATG GCTATTCTGT GTCACACCAG
    CTCTCTGCTT GCTTCCCTTA CCGTTCAGCT GATGTCACCA CCAGCCCTTA TGTGGACACA
    CAGAATAGCT ATGGGGGTTC TACTTCCACC CCTTCCTCCA GCTCTCGGCT GATGTTGTTC
    AGTCCACCTG GGCAGCTACC TCAGAGTTTG AGTTCCCCAT CAACACGGCT GTTACCTGAG
    CCGCTGCAAG TACGGTGCCA CTCAGCATGC CTTCACCTCC TCTACCTTCC GGCTAGTCTC
    TGGAGCCCAT CTGCGGTCTG TGGTATGACC ACTCCTCCTA CGTCTCCTGG AAATGTCCCA
    GCTGATTTGT CACATCCGTA TAGTAAAGCC TTTGGTACCA CTGGTGGAAA AGGAACTCCT
    TCAGGAACCC CAGCGACCTC TCCTCCTCCA GCCCCACCTT GTCCCCAAGA TGACTGTGTG
    CATGGTTCAG CAGCCCAGGC CTCAGCCACA GCCCCCAGGA AGGAAGAAAG AGCAGATTCC
    TCAAGGCCTT ACCTTCACAG ACAGTCAAAC GACCGAGGAT TAGAGGATCC ACCTGGAAGC
    AAAGGTTCCG TTACTCTGAG GAATCTACCT GATTTCCTAG GTGATCTGGC TTCTGAGGAA
    GACAGTATCG AGAAAGATAA GGAAGAAGCT GCAATATCTA AAGAGCTTTC TGAGATCACT
    ACTGCAGAGG CGGATCCTGT AGTTCCTCGA GGGGGCTTTG ACTCTCCCTT CTACCGAGAC
    AGTCTCTCTG GCTCTCAGCG GAAGACTCAT TCGGCAGCCT CTGGGACTCA GGGCTCCAGC
    GTGAACCCTG AGCCTTTGCA CTCCTCCCTG GACAAACATG GGCCTGACAC ACCAAAGCAA
    GCCTTTACTC CCATAGACCC ACCCTCTGGC AGTGCTGATG TCAGTCCCGC TGGGGACAGG
    GATCGCCAGA CTTCTCTGGA GACCAGTATC CTCACTCCCA GCCCTTGCAA AATCCCACCT
    CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG CTTCCCCCAT ATGATCATCT CTTTGAGGTG
    GCCTTGCCAA AGACTGCCTG TCACTTTGTC AGCAAGAAGA CTGAGGAGCT GTTGAAGAAA
    GTGAAAGGAA ACCCTGAGGA AGACTGTGTG CCCTCTACCT CCCCAATGGA AGTACTGGAC
    AGACTGATAG AGCAGGGAGC AGGTGCGCAC AGCAAGGAGC TGAGCAGGTT GTCCCTGCCC
    AGCAAGTCTG TTGACTGGAC CCACTTTGGA GATGAGCTCC GGACCCTCCG AGACCAGTTG
    CTTTTACTGC ACAATCAGCT GCTGTACGAG CGCTTCAAGC GGCAGCAGCA TGCACTCAGG
    AACAGAAGGC TCCTGCGCAA GGTGATCCGA GCAGCGGCTC TGGAGGAACA CAATGCAGCA
    ATGAAAGATC AGTTGAAGTT ACAAGAGAAG GACATACAGA TGTGGAAGGT GAGTCTGCAG
    AAAGAACAAG CCCGATACAG TCAGCTTCAG GAACAACGTG ACACCATGGT GACCCAACTG
    CATAGCCAGA TCAGACAGCT ACAGCATGAC CGAGAAGAAT TCTACAACCA GAGTCAGGAG
    TTACAGACAA AGCTGGAGGA CTGCAGAAAC ATGATTGCGG AGCTTCGGGT GGAGCTGAAG
    AAGGCTAACA ACAAGGTGTG CCACACTGAG CTGCTGCTCA GCCAGGTCTC TCAGAAGCTC
    TCCAACAGTG AGTCAGTGCA GCAGCAGATG GAGTTCTTGA ATAGGCAGCT CCTGGTGCTC
    GGGGAGGTCA ATGAGCTGTA CCTGGAGCAG CTGCAGAGCA AGCATCCTGA CACCACCAAG
    GAAGTAGAAA TGATGAAAAC TGCATATCGG AAAGAGCTAG AGAAAAACAG AAGCCACCTT
    CTCCAGCAGA ACCAAAGGTT GGACGCCTCA CAGAGGCGAG TTTTGGAACT GGAGTCTCTT
    CTGGCCAAGA AAGACCACCT TCTCCTAGAA CAGAAGAAAT ATCTTGAGGA TGTCAAGAGC
    CAGGCGAGTG GACAGCTGCT GGCTGCAGAG AGCAGGTATG AGGCTCAGAG AAAGATCACC
    CGGGTGTTGG AACTGGAGAT CCTAGACTTG TATGGCAGGT TGGAAAAAGA TGGCCGCCTA
    CGGAAACTAG AAGAGGACAG AGCAGAGGCA GCAGAGGCAG CAGAAGAGAG GCTTGACTGT
    TGTAGTGATG GATGCACAGA TTCCTTGGTA GGACATAATG AAGAGGCTTC TGGTCACAAT
    GGTGAGACCA GGACCTCCAG ACCTGGTGGC ACCCGGGCCA GCTGTGGAGG TAGAGTCACT
    GGAGGCAGCA GCAGCAGCAG CAGTGAGCTT TCCACTCCAG AGAAACCCCC GAGCCAGAGG
    TTCAGCAGCC GGTGGGAACC TGCCCTGGGC GAGCCCTCCA GCAGCATCCC CACCACTGTT
    GGCTCACTTC CCAGTTCCAA AAGCTTCCTG GGCATGAAGG CCCGGGAGCT GTTCCGTAAT
    AAGAGCGAGA GCCAGTGTGA TGAGGACAGC GTGACCATGA GTAGCAGCAG CCTTTCTGAG
    ACCCTGAAGA CAGAACTGGG CAAGGACTCG GGCACAGAAA ACAAGACTTC CCTGAGTCTA
    GATGCCCCAC ACCCATCTTC CCCAAACTCA GACAATGTGG GGCAGCTCCA CATCATGGAC
    TACAATGAGA CTCATCCTGA ACACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.