tsc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tsc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tsc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa64231 XM_031890980.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64231 XM_031890978.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64231 XM_031890979.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64232 XM_031890981.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64233 XM_031890982.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64234 XM_031890983.1
Latest version!
Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa15213 XM_002944635.3
Latest version!
Xenopus tropicalis tuberous sclerosis 1 (tsc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa64231
    Clone ID Related Accession (Same CDS sequence) XM_031890980.1 , XM_031890978.1 , XM_031890979.1
    Accession Version XM_031890980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746840.1
    CDS201..3554
    Translation

    Target ORF information:

    RefSeq Version XM_031890980.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64231
    Clone ID Related Accession (Same CDS sequence) XM_031890980.1 , XM_031890978.1 , XM_031890979.1
    Accession Version XM_031890978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746838.1
    CDS325..3678
    Translation

    Target ORF information:

    RefSeq Version XM_031890978.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64231
    Clone ID Related Accession (Same CDS sequence) XM_031890980.1 , XM_031890978.1 , XM_031890979.1
    Accession Version XM_031890979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746839.1
    CDS189..3542
    Translation

    Target ORF information:

    RefSeq Version XM_031890979.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAACA GGTTGTCTCT ACCCAGCAAA
    GCCGCTGACT GGACTCACTT TGGAGGTTCC CCGCCAACGG ACGAGATCCA CGTACTTCGC
    AACCAGTTGG TTCTGCTTCA CAACCAGCTT TTGTACGAGA GGTTCAAAAG GCAGCAGCAC
    GCCCAGCGCA ACCGGCGCCT CCTGCGCAAA GTGATCAAGG TGACGTCTCT GGAGGAACAG
    AATGCTGCTA TGAAGAACCA GTTAAAGCTG CAGGAGTTGG ATATGCACTC TATCAAACAG
    AGCCTCTTTC AGGAACAGAC ACGGCACCGG CGGCTTCAGG AAGAGCGCGA CGCAGTGGTC
    ACACAGCTGC GCAGCCAGAT CCGCCAGTTG CAGCACGACC GAGATCTGTT TTATAACCAG
    AGCCAGGAGC TGCAGACCAA GCTAGAGGAT TGCCGCACCA CCATTGCAGA TCTACGTCTG
    GAGGTGAAAC AAGCCAATAA TAAAGTGTGC CATACAGAGC TTCGGCTAAG CCAGGTTTCC
    CAGAAGCTCT CCAACAGTGA GTCCGTGCAG CATCAGATGG AGTTTCTGAA CCGTCAGCTG
    CTGGTCCTGG GGGAGGTGAA TGAGTTGTAC GTGGAGCAGT TGCAGCAAAA GCAGACAAAT
    AGCACAAAGG AAGTGGAGAT GTTGCAGACT TCCTTTAAGA AGGACCTGGA GAAGGCCAGA
    CTGAATGCCG TGCAGCTGAG CCAGAGGCTG GAGTGCGCCC AGAAACGCAT AGTGGAGCTG
    GAAGCTCAGC TGGCCAAGAA AGAATCCCTG TTTCAAGAAC AGAAAAAATT CATAGAGCAG
    ATTAAGGACA AGAGCAGAGG GCAACTGCAA GCCGCCGAGA GCCGATACCT CGCTCAGAAG
    AGGATAAGCC AGGTCTTTGA AGTGAAAATC CTCAGTCTGT ACAACAGGTT GGAGAAGGAA
    GGGCTTCTGA AAAGTCAAGG TGAAGAGAAA GCGGAGACGG CAGAAGCTGC AGAAGAAAGG
    TGTGACCTCA GCCTCGCAAG TGTTTACAGC CCTGAAGCTG GCATCATAGA AGAAATGAAC
    GGTAGCAGCG AGAATAATTT CCCCACCAAC CGCACAAGCT CGTCCAGCAG CCCGAGCAGC
    AGCACCCTCT CCACCCCGGA GAAAGGCACA AACTTGCAGC TCAGCCGTCG CCAGTTACTG
    GAGATCCCTC CCAGCGGGCA CTTGACCATC GGCTCCTACC CCAACGCCAA AAGCTTCCTG
    GGCATCCGGG CCCGCGAGCT GTTCCGCAAC AAGAGCGAGA GCCACTGCGA CGAGGAAAGC
    ATCAGCAGCC TCTCGGAGAT CCTGAAAAGC GAACTCTGCC AGGAGCTTAA CCCAGAGACG
    AACCCCCAAA ATCCAGATTG TGTGAGGAAT CTTCAGATCA TGGACTACGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64232
    Clone ID Related Accession (Same CDS sequence) XM_031890981.1
    Accession Version XM_031890981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAAGT TGTCTCTACC CAGCAAAGCC
    GCTGACTGGA CTCACTTTGG AGGTTCCCCG CCAACGGACG AGATCCACGT ACTTCGCAAC
    CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TACGAGAGGT TCAAAAGGCA GCAGCACGCC
    CAGCGCAACC GGCGCCTCCT GCGCAAAGTG ATCAAGGTGA CGTCTCTGGA GGAACAGAAT
    GCTGCTATGA AGAACCAGTT AAAGCTGCAG GAGTTGGATA TGCACTCTAT CAAACAGAGC
    CTCTTTCAGG AACAGACACG GCACCGGCGG CTTCAGGAAG AGCGCGACGC AGTGGTCACA
    CAGCTGCGCA GCCAGATCCG CCAGTTGCAG CACGACCGAG ATCTGTTTTA TAACCAGAGC
    CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATCT ACGTCTGGAG
    GTGAAACAAG CCAATAATAA AGTGTGCCAT ACAGAGCTTC GGCTAAGCCA GGTTTCCCAG
    AAGCTCTCCA ACAGTGAGTC CGTGCAGCAT CAGATGGAGT TTCTGAACCG TCAGCTGCTG
    GTCCTGGGGG AGGTGAATGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAATAGC
    ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAGAAGG ACCTGGAGAA GGCCAGACTG
    AATGCCGTGC AGCTGAGCCA GAGGCTGGAG TGCGCCCAGA AACGCATAGT GGAGCTGGAA
    GCTCAGCTGG CCAAGAAAGA ATCCCTGTTT CAAGAACAGA AAAAATTCAT AGAGCAGATT
    AAGGACAAGA GCAGAGGGCA ACTGCAAGCC GCCGAGAGCC GATACCTCGC TCAGAAGAGG
    ATAAGCCAGG TCTTTGAAGT GAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG
    CTTCTGAAAA GTCAAGGTGA AGAGAAAGCG GAGACGGCAG AAGCTGCAGA AGAAAGGTGT
    GACCTCAGCC TCGCAAGTGT TTACAGCCCT GAAGCTGGCA TCATAGAAGA AATGAACGGT
    AGCAGCGAGA ATAATTTCCC CACCAACCGC ACAAGCTCGT CCAGCAGCCC GAGCAGCAGC
    ACCCTCTCCA CCCCGGAGAA AGGCACAAAC TTGCAGCTCA GCCGTCGCCA GTTACTGGAG
    ATCCCTCCCA GCGGGCACTT GACCATCGGC TCCTACCCCA ACGCCAAAAG CTTCCTGGGC
    ATCCGGGCCC GCGAGCTGTT CCGCAACAAG AGCGAGAGCC ACTGCGACGA GGAAAGCATC
    AGCAGCCTCT CGGAGATCCT GAAAAGCGAA CTCTGCCAGG AGCTTAACCC AGAGACGAAC
    CCCCAAAATC CAGATTGTGT GAGGAATCTT CAGATCATGG ACTACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746841.1
    CDS325..3675
    Translation

    Target ORF information:

    RefSeq Version XM_031890981.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAAGT TGTCTCTACC CAGCAAAGCC
    GCTGACTGGA CTCACTTTGG AGGTTCCCCG CCAACGGACG AGATCCACGT ACTTCGCAAC
    CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TACGAGAGGT TCAAAAGGCA GCAGCACGCC
    CAGCGCAACC GGCGCCTCCT GCGCAAAGTG ATCAAGGTGA CGTCTCTGGA GGAACAGAAT
    GCTGCTATGA AGAACCAGTT AAAGCTGCAG GAGTTGGATA TGCACTCTAT CAAACAGAGC
    CTCTTTCAGG AACAGACACG GCACCGGCGG CTTCAGGAAG AGCGCGACGC AGTGGTCACA
    CAGCTGCGCA GCCAGATCCG CCAGTTGCAG CACGACCGAG ATCTGTTTTA TAACCAGAGC
    CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATCT ACGTCTGGAG
    GTGAAACAAG CCAATAATAA AGTGTGCCAT ACAGAGCTTC GGCTAAGCCA GGTTTCCCAG
    AAGCTCTCCA ACAGTGAGTC CGTGCAGCAT CAGATGGAGT TTCTGAACCG TCAGCTGCTG
    GTCCTGGGGG AGGTGAATGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAATAGC
    ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAGAAGG ACCTGGAGAA GGCCAGACTG
    AATGCCGTGC AGCTGAGCCA GAGGCTGGAG TGCGCCCAGA AACGCATAGT GGAGCTGGAA
    GCTCAGCTGG CCAAGAAAGA ATCCCTGTTT CAAGAACAGA AAAAATTCAT AGAGCAGATT
    AAGGACAAGA GCAGAGGGCA ACTGCAAGCC GCCGAGAGCC GATACCTCGC TCAGAAGAGG
    ATAAGCCAGG TCTTTGAAGT GAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG
    CTTCTGAAAA GTCAAGGTGA AGAGAAAGCG GAGACGGCAG AAGCTGCAGA AGAAAGGTGT
    GACCTCAGCC TCGCAAGTGT TTACAGCCCT GAAGCTGGCA TCATAGAAGA AATGAACGGT
    AGCAGCGAGA ATAATTTCCC CACCAACCGC ACAAGCTCGT CCAGCAGCCC GAGCAGCAGC
    ACCCTCTCCA CCCCGGAGAA AGGCACAAAC TTGCAGCTCA GCCGTCGCCA GTTACTGGAG
    ATCCCTCCCA GCGGGCACTT GACCATCGGC TCCTACCCCA ACGCCAAAAG CTTCCTGGGC
    ATCCGGGCCC GCGAGCTGTT CCGCAACAAG AGCGAGAGCC ACTGCGACGA GGAAAGCATC
    AGCAGCCTCT CGGAGATCCT GAAAAGCGAA CTCTGCCAGG AGCTTAACCC AGAGACGAAC
    CCCCAAAATC CAGATTGTGT GAGGAATCTT CAGATCATGG ACTACGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64233
    Clone ID Related Accession (Same CDS sequence) XM_031890982.1
    Accession Version XM_031890982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG CTGCCATATG CCAGGAGCTG TCTCAGATCA CCACGGCTGA CCCCGAGCCG
    ACCGTGCTAA GAGGTGGCTT TGATTCCCCT TTCTCTCTCC GCTGTGACAG TCAGCCCAGC
    TCTCAGAAGA AGTTTCCCTC CTATGGCGTT GGCCACATCC CTGAACATTC TGCCCCCAAC
    AAGCTCCACT ACAGTGCACC CCATGATAGC TCCAAACAGG CGTTCACTCC CATTGAACCG
    CCCAACGGCA CGGAGAATGC CGCCGACTCA GGGAAGCCAC CTTCTAACAA CTCTTTGCCC
    AGCGCTGTGG ACATCTTCAC ACCTCAGGTT CAAAGAAACG TATCCTGCTT CAGCTTGCCT
    CCCTACGACC ATCTCTTTGA GCTGGCCTTG CCCAAAACCG CCAACATGTT CGTCTGCAGG
    AAGAACGAGG AGCTGTGCAG AAGAGGCAAG GGAAGCCTTG AGGAGGGCGA AGAGGGCGCC
    TTCTCCGTTT CCCCGGCTGA GGTGCTGGAC CGGCTCATTC AGTTGGGAGC AGATGCCCAC
    ACCAAAGAGC TGAGCAACAG GTTGTCTCTA CCCAGCAAAG CCGCTGACTG GACTCACTTT
    GGAGGTTCCC CGCCAACGGA CGAGATCCAC GTACTTCGCA ACCAGTTGGT TCTGCTTCAC
    AACCAGCTTT TGTACGAGAG GTTCAAAAGG CAGCAGCACG CCCAGCGCAA CCGGCGCCTC
    CTGCGCAAAG TGATCAAGGT GACGTCTCTG GAGGAACAGA ATGCTGCTAT GAAGAACCAG
    TTAAAGCTGC AGGAGTTGGA TATGCACTCT ATCAAACAGA GCCTCTTTCA GGAACAGACA
    CGGCACCGGC GGCTTCAGGA AGAGCGCGAC GCAGTGGTCA CACAGCTGCG CAGCCAGATC
    CGCCAGTTGC AGCACGACCG AGATCTGTTT TATAACCAGA GCCAGGAGCT GCAGACCAAG
    CTAGAGGATT GCCGCACCAC CATTGCAGAT CTACGTCTGG AGGTGAAACA AGCCAATAAT
    AAAGTGTGCC ATACAGAGCT TCGGCTAAGC CAGGTTTCCC AGAAGCTCTC CAACAGTGAG
    TCCGTGCAGC ATCAGATGGA GTTTCTGAAC CGTCAGCTGC TGGTCCTGGG GGAGGTGAAT
    GAGTTGTACG TGGAGCAGTT GCAGCAAAAG CAGACAAATA GCACAAAGGA AGTGGAGATG
    TTGCAGACTT CCTTTAAGAA GGACCTGGAG AAGGCCAGAC TGAATGCCGT GCAGCTGAGC
    CAGAGGCTGG AGTGCGCCCA GAAACGCATA GTGGAGCTGG AAGCTCAGCT GGCCAAGAAA
    GAATCCCTGT TTCAAGAACA GAAAAAATTC ATAGAGCAGA TTAAGGACAA GAGCAGAGGG
    CAACTGCAAG CCGCCGAGAG CCGATACCTC GCTCAGAAGA GGATAAGCCA GGTCTTTGAA
    GTGAAAATCC TCAGTCTGTA CAACAGGTTG GAGAAGGAAG GGCTTCTGAA AAGTCAAGGT
    GAAGAGAAAG CGGAGACGGC AGAAGCTGCA GAAGAAAGGT GTGACCTCAG CCTCGCAAGT
    GTTTACAGCC CTGAAGCTGG CATCATAGAA GAAATGAACG GTAGCAGCGA GAATAATTTC
    CCCACCAACC GCACAAGCTC GTCCAGCAGC CCGAGCAGCA GCACCCTCTC CACCCCGGAG
    AAAGGCACAA ACTTGCAGCT CAGCCGTCGC CAGTTACTGG AGATCCCTCC CAGCGGGCAC
    TTGACCATCG GCTCCTACCC CAACGCCAAA AGCTTCCTGG GCATCCGGGC CCGCGAGCTG
    TTCCGCAACA AGAGCGAGAG CCACTGCGAC GAGGAAAGCA TCAGCAGCCT CTCGGAGATC
    CTGAAAAGCG AACTCTGCCA GGAGCTTAAC CCAGAGACGA ACCCCCAAAA TCCAGATTGT
    GTGAGGAATC TTCAGATCAT GGACTACGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746842.1
    CDS325..3657
    Translation

    Target ORF information:

    RefSeq Version XM_031890982.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG CTGCCATATG CCAGGAGCTG TCTCAGATCA CCACGGCTGA CCCCGAGCCG
    ACCGTGCTAA GAGGTGGCTT TGATTCCCCT TTCTCTCTCC GCTGTGACAG TCAGCCCAGC
    TCTCAGAAGA AGTTTCCCTC CTATGGCGTT GGCCACATCC CTGAACATTC TGCCCCCAAC
    AAGCTCCACT ACAGTGCACC CCATGATAGC TCCAAACAGG CGTTCACTCC CATTGAACCG
    CCCAACGGCA CGGAGAATGC CGCCGACTCA GGGAAGCCAC CTTCTAACAA CTCTTTGCCC
    AGCGCTGTGG ACATCTTCAC ACCTCAGGTT CAAAGAAACG TATCCTGCTT CAGCTTGCCT
    CCCTACGACC ATCTCTTTGA GCTGGCCTTG CCCAAAACCG CCAACATGTT CGTCTGCAGG
    AAGAACGAGG AGCTGTGCAG AAGAGGCAAG GGAAGCCTTG AGGAGGGCGA AGAGGGCGCC
    TTCTCCGTTT CCCCGGCTGA GGTGCTGGAC CGGCTCATTC AGTTGGGAGC AGATGCCCAC
    ACCAAAGAGC TGAGCAACAG GTTGTCTCTA CCCAGCAAAG CCGCTGACTG GACTCACTTT
    GGAGGTTCCC CGCCAACGGA CGAGATCCAC GTACTTCGCA ACCAGTTGGT TCTGCTTCAC
    AACCAGCTTT TGTACGAGAG GTTCAAAAGG CAGCAGCACG CCCAGCGCAA CCGGCGCCTC
    CTGCGCAAAG TGATCAAGGT GACGTCTCTG GAGGAACAGA ATGCTGCTAT GAAGAACCAG
    TTAAAGCTGC AGGAGTTGGA TATGCACTCT ATCAAACAGA GCCTCTTTCA GGAACAGACA
    CGGCACCGGC GGCTTCAGGA AGAGCGCGAC GCAGTGGTCA CACAGCTGCG CAGCCAGATC
    CGCCAGTTGC AGCACGACCG AGATCTGTTT TATAACCAGA GCCAGGAGCT GCAGACCAAG
    CTAGAGGATT GCCGCACCAC CATTGCAGAT CTACGTCTGG AGGTGAAACA AGCCAATAAT
    AAAGTGTGCC ATACAGAGCT TCGGCTAAGC CAGGTTTCCC AGAAGCTCTC CAACAGTGAG
    TCCGTGCAGC ATCAGATGGA GTTTCTGAAC CGTCAGCTGC TGGTCCTGGG GGAGGTGAAT
    GAGTTGTACG TGGAGCAGTT GCAGCAAAAG CAGACAAATA GCACAAAGGA AGTGGAGATG
    TTGCAGACTT CCTTTAAGAA GGACCTGGAG AAGGCCAGAC TGAATGCCGT GCAGCTGAGC
    CAGAGGCTGG AGTGCGCCCA GAAACGCATA GTGGAGCTGG AAGCTCAGCT GGCCAAGAAA
    GAATCCCTGT TTCAAGAACA GAAAAAATTC ATAGAGCAGA TTAAGGACAA GAGCAGAGGG
    CAACTGCAAG CCGCCGAGAG CCGATACCTC GCTCAGAAGA GGATAAGCCA GGTCTTTGAA
    GTGAAAATCC TCAGTCTGTA CAACAGGTTG GAGAAGGAAG GGCTTCTGAA AAGTCAAGGT
    GAAGAGAAAG CGGAGACGGC AGAAGCTGCA GAAGAAAGGT GTGACCTCAG CCTCGCAAGT
    GTTTACAGCC CTGAAGCTGG CATCATAGAA GAAATGAACG GTAGCAGCGA GAATAATTTC
    CCCACCAACC GCACAAGCTC GTCCAGCAGC CCGAGCAGCA GCACCCTCTC CACCCCGGAG
    AAAGGCACAA ACTTGCAGCT CAGCCGTCGC CAGTTACTGG AGATCCCTCC CAGCGGGCAC
    TTGACCATCG GCTCCTACCC CAACGCCAAA AGCTTCCTGG GCATCCGGGC CCGCGAGCTG
    TTCCGCAACA AGAGCGAGAG CCACTGCGAC GAGGAAAGCA TCAGCAGCCT CTCGGAGATC
    CTGAAAAGCG AACTCTGCCA GGAGCTTAAC CCAGAGACGA ACCCCCAAAA TCCAGATTGT
    GTGAGGAATC TTCAGATCAT GGACTACGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64234
    Clone ID Related Accession (Same CDS sequence) XM_031890983.1
    Accession Version XM_031890983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG CTGCCATATG CCAGGAGCTG TCTCAGATCA CCACGGCTGA CCCCGAGCCG
    ACCGTGCTAA GAGGTGGCTT TGATTCCCCT TTCTCTCTCC GCTGTGACAG TCAGCCCAGC
    TCTCAGAAGA AGTTTCCCTC CTATGGCGTT GGCCACATCC CTGAACATTC TGCCCCCAAC
    AAGCTCCACT ACAGTGCACC CCATGATAGC TCCAAACAGG CGTTCACTCC CATTGAACCG
    CCCAACGGCA CGGAGAATGC CGCCGACTCA GGGAAGCCAC CTTCTAACAA CTCTTTGCCC
    AGCGCTGTGG ACATCTTCAC ACCTCAGGTT CAAAGAAACG TATCCTGCTT CAGCTTGCCT
    CCCTACGACC ATCTCTTTGA GCTGGCCTTG CCCAAAACCG CCAACATGTT CGTCTGCAGG
    AAGAACGAGG AGCTGTGCAG AAGAGGCAAG GGAAGCCTTG AGGAGGGCGA AGAGGGCGCC
    TTCTCCGTTT CCCCGGCTGA GGTGCTGGAC CGGCTCATTC AGTTGGGAGC AGATGCCCAC
    ACCAAAGAGC TGAGCAAGTT GTCTCTACCC AGCAAAGCCG CTGACTGGAC TCACTTTGGA
    GGTTCCCCGC CAACGGACGA GATCCACGTA CTTCGCAACC AGTTGGTTCT GCTTCACAAC
    CAGCTTTTGT ACGAGAGGTT CAAAAGGCAG CAGCACGCCC AGCGCAACCG GCGCCTCCTG
    CGCAAAGTGA TCAAGGTGAC GTCTCTGGAG GAACAGAATG CTGCTATGAA GAACCAGTTA
    AAGCTGCAGG AGTTGGATAT GCACTCTATC AAACAGAGCC TCTTTCAGGA ACAGACACGG
    CACCGGCGGC TTCAGGAAGA GCGCGACGCA GTGGTCACAC AGCTGCGCAG CCAGATCCGC
    CAGTTGCAGC ACGACCGAGA TCTGTTTTAT AACCAGAGCC AGGAGCTGCA GACCAAGCTA
    GAGGATTGCC GCACCACCAT TGCAGATCTA CGTCTGGAGG TGAAACAAGC CAATAATAAA
    GTGTGCCATA CAGAGCTTCG GCTAAGCCAG GTTTCCCAGA AGCTCTCCAA CAGTGAGTCC
    GTGCAGCATC AGATGGAGTT TCTGAACCGT CAGCTGCTGG TCCTGGGGGA GGTGAATGAG
    TTGTACGTGG AGCAGTTGCA GCAAAAGCAG ACAAATAGCA CAAAGGAAGT GGAGATGTTG
    CAGACTTCCT TTAAGAAGGA CCTGGAGAAG GCCAGACTGA ATGCCGTGCA GCTGAGCCAG
    AGGCTGGAGT GCGCCCAGAA ACGCATAGTG GAGCTGGAAG CTCAGCTGGC CAAGAAAGAA
    TCCCTGTTTC AAGAACAGAA AAAATTCATA GAGCAGATTA AGGACAAGAG CAGAGGGCAA
    CTGCAAGCCG CCGAGAGCCG ATACCTCGCT CAGAAGAGGA TAAGCCAGGT CTTTGAAGTG
    AAAATCCTCA GTCTGTACAA CAGGTTGGAG AAGGAAGGGC TTCTGAAAAG TCAAGGTGAA
    GAGAAAGCGG AGACGGCAGA AGCTGCAGAA GAAAGGTGTG ACCTCAGCCT CGCAAGTGTT
    TACAGCCCTG AAGCTGGCAT CATAGAAGAA ATGAACGGTA GCAGCGAGAA TAATTTCCCC
    ACCAACCGCA CAAGCTCGTC CAGCAGCCCG AGCAGCAGCA CCCTCTCCAC CCCGGAGAAA
    GGCACAAACT TGCAGCTCAG CCGTCGCCAG TTACTGGAGA TCCCTCCCAG CGGGCACTTG
    ACCATCGGCT CCTACCCCAA CGCCAAAAGC TTCCTGGGCA TCCGGGCCCG CGAGCTGTTC
    CGCAACAAGA GCGAGAGCCA CTGCGACGAG GAAAGCATCA GCAGCCTCTC GGAGATCCTG
    AAAAGCGAAC TCTGCCAGGA GCTTAACCCA GAGACGAACC CCCAAAATCC AGATTGTGTG
    AGGAATCTTC AGATCATGGA CTACGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746843.1
    CDS325..3654
    Translation

    Target ORF information:

    RefSeq Version XM_031890983.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TSC complex subunit 1 (tsc1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG CTGCCATATG CCAGGAGCTG TCTCAGATCA CCACGGCTGA CCCCGAGCCG
    ACCGTGCTAA GAGGTGGCTT TGATTCCCCT TTCTCTCTCC GCTGTGACAG TCAGCCCAGC
    TCTCAGAAGA AGTTTCCCTC CTATGGCGTT GGCCACATCC CTGAACATTC TGCCCCCAAC
    AAGCTCCACT ACAGTGCACC CCATGATAGC TCCAAACAGG CGTTCACTCC CATTGAACCG
    CCCAACGGCA CGGAGAATGC CGCCGACTCA GGGAAGCCAC CTTCTAACAA CTCTTTGCCC
    AGCGCTGTGG ACATCTTCAC ACCTCAGGTT CAAAGAAACG TATCCTGCTT CAGCTTGCCT
    CCCTACGACC ATCTCTTTGA GCTGGCCTTG CCCAAAACCG CCAACATGTT CGTCTGCAGG
    AAGAACGAGG AGCTGTGCAG AAGAGGCAAG GGAAGCCTTG AGGAGGGCGA AGAGGGCGCC
    TTCTCCGTTT CCCCGGCTGA GGTGCTGGAC CGGCTCATTC AGTTGGGAGC AGATGCCCAC
    ACCAAAGAGC TGAGCAAGTT GTCTCTACCC AGCAAAGCCG CTGACTGGAC TCACTTTGGA
    GGTTCCCCGC CAACGGACGA GATCCACGTA CTTCGCAACC AGTTGGTTCT GCTTCACAAC
    CAGCTTTTGT ACGAGAGGTT CAAAAGGCAG CAGCACGCCC AGCGCAACCG GCGCCTCCTG
    CGCAAAGTGA TCAAGGTGAC GTCTCTGGAG GAACAGAATG CTGCTATGAA GAACCAGTTA
    AAGCTGCAGG AGTTGGATAT GCACTCTATC AAACAGAGCC TCTTTCAGGA ACAGACACGG
    CACCGGCGGC TTCAGGAAGA GCGCGACGCA GTGGTCACAC AGCTGCGCAG CCAGATCCGC
    CAGTTGCAGC ACGACCGAGA TCTGTTTTAT AACCAGAGCC AGGAGCTGCA GACCAAGCTA
    GAGGATTGCC GCACCACCAT TGCAGATCTA CGTCTGGAGG TGAAACAAGC CAATAATAAA
    GTGTGCCATA CAGAGCTTCG GCTAAGCCAG GTTTCCCAGA AGCTCTCCAA CAGTGAGTCC
    GTGCAGCATC AGATGGAGTT TCTGAACCGT CAGCTGCTGG TCCTGGGGGA GGTGAATGAG
    TTGTACGTGG AGCAGTTGCA GCAAAAGCAG ACAAATAGCA CAAAGGAAGT GGAGATGTTG
    CAGACTTCCT TTAAGAAGGA CCTGGAGAAG GCCAGACTGA ATGCCGTGCA GCTGAGCCAG
    AGGCTGGAGT GCGCCCAGAA ACGCATAGTG GAGCTGGAAG CTCAGCTGGC CAAGAAAGAA
    TCCCTGTTTC AAGAACAGAA AAAATTCATA GAGCAGATTA AGGACAAGAG CAGAGGGCAA
    CTGCAAGCCG CCGAGAGCCG ATACCTCGCT CAGAAGAGGA TAAGCCAGGT CTTTGAAGTG
    AAAATCCTCA GTCTGTACAA CAGGTTGGAG AAGGAAGGGC TTCTGAAAAG TCAAGGTGAA
    GAGAAAGCGG AGACGGCAGA AGCTGCAGAA GAAAGGTGTG ACCTCAGCCT CGCAAGTGTT
    TACAGCCCTG AAGCTGGCAT CATAGAAGAA ATGAACGGTA GCAGCGAGAA TAATTTCCCC
    ACCAACCGCA CAAGCTCGTC CAGCAGCCCG AGCAGCAGCA CCCTCTCCAC CCCGGAGAAA
    GGCACAAACT TGCAGCTCAG CCGTCGCCAG TTACTGGAGA TCCCTCCCAG CGGGCACTTG
    ACCATCGGCT CCTACCCCAA CGCCAAAAGC TTCCTGGGCA TCCGGGCCCG CGAGCTGTTC
    CGCAACAAGA GCGAGAGCCA CTGCGACGAG GAAAGCATCA GCAGCCTCTC GGAGATCCTG
    AAAAGCGAAC TCTGCCAGGA GCTTAACCCA GAGACGAACC CCCAAAATCC AGATTGTGTG
    AGGAATCTTC AGATCATGGA CTACGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa15213
    Clone ID Related Accession (Same CDS sequence) XM_002944635.3
    Accession Version XM_002944635.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product hamartin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002944635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAAGT TGTCTCTACC CAGCAAAGCC
    GCTGACTGGA CTCACTTTGG AGGTTCCCCG CCAACGGACG AGATCCACGT ACTTCGCAAC
    CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TACGAGAGGT TCAAAAGGCA GCAGCACGCC
    CAGCGCAACC GGCGCCTCCT GCGCAAAGTG ATCAAGGTGA CGTCTCTGGA GGAACAGAAT
    GCTGCTATGA AGAACCAGTT AAAGCTGCAG GAGTTGGATA TGCACTCTAT CAAACAGAGC
    CTCTTTCAGG AACAGACACG GCACCGGCGG CTTCAGGAAG AGCGCGACGC AGTGGTCACA
    CAGCTGCGCA GCCAGATCCG CCAGTTGCAG CACGACCGAG ATCTGTTTTA TAACCAGAGC
    CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATCT ACGTCTGGAG
    GTGAAACAAG CCAATAATAA AGTGTGCCAT ACAGAGCTTC GGCTAAGCCA GGTTTCCCAG
    AAGCTCTCCA ACAGTGAGTC CGTGCAGCAT CAGATGGAGT TTCTGAACCG TCAGCTGCTG
    GTCCTGGGGG AGGTGAATGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAATAGC
    ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAGAAGG ACCTGGAGAA GGCCAGACTG
    AATGCCGTGC AGCTGAGCCA GAGGCTGGAG TGCGCCCAGA AACGCATAGT GGAGCTGGAA
    GCTCAGCTGG CCAAGAAAGA ATCCCTGTTT CAAGAACAGA AAAAATTCAT AGAGCAGATT
    AAGGACAAGA GCAGAGGGCA ACTGCAAGCC GCCGAGAGCC GATACCTCGC TCAGAAGAGG
    ATAAGCCAGG TCTTTGAAGT GAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG
    CTTCTGAAAA GTCAAGGTGA AGAGAAAGCG GAGACGGCAG AAGCTGCAGA AGAAAGGTGT
    GACCTCAGCC TCGCAAGTGT TTACAGCCCT GAAGCTGGCA TCATAGAAGA AATGAACGGT
    AGCAGCGAGA ATAATTTCCC CACCAACCGC ACAAGCTCGT CCAGCAGCCC GAGCAGCAGC
    ACCCTCTCCA CCCCGGAGAA AGGCACAAAC TTGCAGCTCA GCCGTCGCCA GTTACTGGAG
    ATCCCTCCCA GCGGGCACTT GACCATCGGC TCCTACCCCA ACGCCAAAAG CTTCCTGGGC
    ATCCGGGCCC GCGAGCTGTT CCGCAACAAG AGCGAGAGCC ACTGCGACGA GGAAAGCATC
    AGCAGCCTCT CGGAGATCCT GAAAAGCGAA CTCTGCCAGG AGCCCATGGG TAGCAAGCAT
    GTAGCTACTG AAATTCCAAA CTGGAGAGCG GCCGAGCAAA AATCGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002944681.2
    CDS337..3687
    Translation

    Target ORF information:

    RefSeq Version XM_002944635.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tuberous sclerosis 1 (tsc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002944635.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAGC AAACCACTGT GTGGGATCAT CTGTATCTGC TGGAATCTTC AGACCTGCAA 
    CAACTGGATC AGATCCATAC GCTTATCCGA GACCATCTAA GTGCAGATCG CTCCCCCATT
    CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAACCGGCT CCGAGCAAGC AATGCTAATC
    CTTTCAATGG TCCAGGAACC TCACGACAAG CACCTTTTGG ATAAGATAAA TGAGTATCTA
    GTTAAAGCTG CCAGCCGGTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG
    CCATCTTGGA AGCACAAACT CTCCCAGGCA CCCGTCCTTC AGTCCTTGCT CAAGTGTCTC
    AAGGTAGACA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG
    CCGATGATTC CACAATCTGG GAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC
    CTGGCAACCT GGTGCTTAAA GAACCCTGGT CGCGTAGCAA AGATATGTCT CGTCCATCTT
    CATGCCAGCG TGTATGCCCT CTTCCACCGG CTTTATGGGA TGTATCCGTG CAATTTTGTG
    TCCTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC
    AAGCCTATGA TGGAGCATGT CCGGATTCAC CCGGAGCTGG TTACTGGCTC GAAAGACCAT
    GAACTGGACC CAAGCAGGTG GAGGAGGCTG GAAACCCATG ACATCGTTGT AGAATGTGCC
    AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT
    CGCCTGCGAC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACCGAAAT CGGTTGTAAT
    TATGGAAGCA ATGCCTCCAC TCCTTTCTCC ACTCCCCGAA TGTGTGCGCA TAGTGCGTCG
    ATTCACTCTG GTGGCATTGA GCTACTGCAG GACAGCATCT GGAGCCCATC CACCATTTGT
    GGAATGACCA CACCACCTAA CTCGCCTGCC AATGTGCTAG CAGATGCGGC TCATTCATCC
    TCTCGTCCCC ATAGCAAAGT CCTCTACACG CCAGGGGGTA AAATAACACC TCTGGGAACA
    CCAGCAACCT CCCCGCCTCC CCCCAGCAAC TCTGAGGAAT GTACGCCAGC CGCCGGCGCT
    TCGCTAACAC TGACACCCCA TAAGAAGAAC GAGTCCTTCG ATCCAGGGAG GTCTTTAGTA
    AACAGACAGC TGCTCAGCAA GGGAGAGAAG AGCGCTGATG AATCACCAAA GACGCAGAAG
    ACCGTGTACC TGAGCGAGCT TCCGGATATC ATTGGGGATT TGGCTGCCGA CCACAATAGC
    ACGGAGGAAG AACTGAAGAG AAACGGTCAT GCTGCCATAT GCCAGGAGCT GTCTCAGATC
    ACCACGGCTG ACCCCGAGCC GACCGTGCTA AGAGGTGGCT TTGATTCCCC TTTCTCTCTC
    CGCTGTGACA GTCAGCCCAG CTCTCAGAAG AAGTTTCCCT CCTATGGCGT TGGCCACATC
    CCTGAACATT CTGCCCCCAA CAAGCTCCAC TACAGTGCAC CCCATGATAG CTCCAAACAG
    GCGTTCACTC CCATTGAACC GCCCAACGGC ACGGAGAATG CCGCCGACTC AGGGAAGCCA
    CCTTCTAACA ACTCTTTGCC CAGCGCTGTG GACATCTTCA CACCTCAGGT TCAAAGAAAC
    GTATCCTGCT TCAGCTTGCC TCCCTACGAC CATCTCTTTG AGCTGGCCTT GCCCAAAACC
    GCCAACATGT TCGTCTGCAG GAAGAACGAG GAGCTGTGCA GAAGAGGCAA GGGAAGCCTT
    GAGGAGGGCG AAGAGGGCGC CTTCTCCGTT TCCCCGGCTG AGGTGCTGGA CCGGCTCATT
    CAGTTGGGAG CAGATGCCCA CACCAAAGAG CTGAGCAAGT TGTCTCTACC CAGCAAAGCC
    GCTGACTGGA CTCACTTTGG AGGTTCCCCG CCAACGGACG AGATCCACGT ACTTCGCAAC
    CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TACGAGAGGT TCAAAAGGCA GCAGCACGCC
    CAGCGCAACC GGCGCCTCCT GCGCAAAGTG ATCAAGGTGA CGTCTCTGGA GGAACAGAAT
    GCTGCTATGA AGAACCAGTT AAAGCTGCAG GAGTTGGATA TGCACTCTAT CAAACAGAGC
    CTCTTTCAGG AACAGACACG GCACCGGCGG CTTCAGGAAG AGCGCGACGC AGTGGTCACA
    CAGCTGCGCA GCCAGATCCG CCAGTTGCAG CACGACCGAG ATCTGTTTTA TAACCAGAGC
    CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATCT ACGTCTGGAG
    GTGAAACAAG CCAATAATAA AGTGTGCCAT ACAGAGCTTC GGCTAAGCCA GGTTTCCCAG
    AAGCTCTCCA ACAGTGAGTC CGTGCAGCAT CAGATGGAGT TTCTGAACCG TCAGCTGCTG
    GTCCTGGGGG AGGTGAATGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAATAGC
    ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAGAAGG ACCTGGAGAA GGCCAGACTG
    AATGCCGTGC AGCTGAGCCA GAGGCTGGAG TGCGCCCAGA AACGCATAGT GGAGCTGGAA
    GCTCAGCTGG CCAAGAAAGA ATCCCTGTTT CAAGAACAGA AAAAATTCAT AGAGCAGATT
    AAGGACAAGA GCAGAGGGCA ACTGCAAGCC GCCGAGAGCC GATACCTCGC TCAGAAGAGG
    ATAAGCCAGG TCTTTGAAGT GAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG
    CTTCTGAAAA GTCAAGGTGA AGAGAAAGCG GAGACGGCAG AAGCTGCAGA AGAAAGGTGT
    GACCTCAGCC TCGCAAGTGT TTACAGCCCT GAAGCTGGCA TCATAGAAGA AATGAACGGT
    AGCAGCGAGA ATAATTTCCC CACCAACCGC ACAAGCTCGT CCAGCAGCCC GAGCAGCAGC
    ACCCTCTCCA CCCCGGAGAA AGGCACAAAC TTGCAGCTCA GCCGTCGCCA GTTACTGGAG
    ATCCCTCCCA GCGGGCACTT GACCATCGGC TCCTACCCCA ACGCCAAAAG CTTCCTGGGC
    ATCCGGGCCC GCGAGCTGTT CCGCAACAAG AGCGAGAGCC ACTGCGACGA GGAAAGCATC
    AGCAGCCTCT CGGAGATCCT GAAAAGCGAA CTCTGCCAGG AGCCCATGGG TAGCAAGCAT
    GTAGCTACTG AAATTCCAAA CTGGAGAGCG GCCGAGCAAA AATCGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.